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Ana Filipa Fernandes da Cruz has a Master degree in Pharmaceutical Sciences (University of Coimbra) and currently she is enrolled in the PhD program in Pharmaceutical Sciences, specialization in Pharmaceutical Technology, at the Center for Neuroscience and Cell Biology (University of Coimbra). Throughout the PhD, she obtained the experience at molecular and cellular level that includes, cell culture (cancer cell lines of diverse histological origin and cancer stem cells), cytotoxicity assays (resazurin reduction assay and LIVE/DEAD cell viability assay), Western Blotting, Flow Cytometry, quantitative RT-PCR and cellular migration. Moreover, Ana Cruz has experience in liposome production (functionalized and/or fluorescent and/or loaded with drugs). Also, she has a significant level of autonomy, at the various levels of the scientific activity and know to carefully plan the experiments. Ana Cruz has already several poster presentations, scientific peer-reviewed papers and oral communications in her curriculum vitae.
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Identification

Personal identification

Full name
Ana Filipa Fernandes da Cruz

Citation names

  • Cruz, Ana F

Author identifiers

Ciência ID
321D-3A30-6F31
ORCID iD
0000-0002-3404-5755

Email addresses

  • ana.filipa.cruz@cnc.uc.pt (Professional)

Addresses

  • Vectors and Gene Therapy Lab, Center for Neuroscience and Cell Biology (CNC). Rua Larga, Faculdade de Medicina, Pólo I, 2º andar, 3004-504, Coimbra, Coimbra, Portugal (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
English Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2013/02/01 - 2022/07/28
Concluded
 Doutoramento em Ciências Farmacêuticas (Doutoramento)
Universidade de Coimbra Faculdade de Farmácia, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2014/01/23
Concluded
 Curso de Ciências de Animais de Laboratório, categoria B* (Outros)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2012
Concluded
 Mestrado Integrado em Ciências Farmacêuticas (Mestrado)
Universidade de Coimbra Faculdade de Farmácia, Portugal
"n/a" (THESIS/DISSERTATION)
 17 valores
Affiliation

Science

Category
Host institution 		Employer
2013/02/01 - Current Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Others

Category
Host institution 		Employer
2013/02/01 - Current PhD student Universidade de Coimbra Faculdade de Farmácia, Portugal
Projects

Grant

Designation Funders
2017 - Current HealthyAging 2020
CENTRO-01-0145-FEDER-000012
Research Fellow
CNC.IBILI, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Ongoing
2017/07/01 - 2021/03/31 CANCEL_STEM_Estaminalidade das células do cancro: um desafio e uma oportunidade para avançar no tratamento em Oncologia¿
SAICTPAC/0022/2015 (POCI-01-0145-FEDER-016390)
Research Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Ongoing
2016 - 2020 Towards a single therapy against triple negative breast cancer and neuroblastoma by nucleolin-mediated multicellular targeting with a synergistic drug combination.
EN/Med/0005/2015
Research Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Concluded
2013/02/01 - 2017/01/31 Insights into the mechanisms of dual targeted-triggered release lipid-based nanoparticles.
SFRH/BD/85554/2012
PhD Student Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Universidade de Coimbra Faculdade de Farmácia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2020/04 - 2020/06 COVID-19 Lab @ CNC / ViraVector
NA
Research Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Ongoing
Outputs

Publications

Book chapter
  1. Cruz, Ana Filipa; Fonseca, Nuno A.; Gregório, Ana C.; Moura, Vera; Simões, Sérgio; Moreira, João Nuno. "Moving Liposome Technology from the Bench to the Oncological Patient: Towards Performance-by-Design". In Particles and Nanoparticles in Pharmaceutical Products, 171-211. Springer International Publishing, 2018.
    10.1007/978-3-319-94174-5_4
  2. Fonseca, Nuno A.; Lopes, Rui M.; Cruz, Ana F.; Gregório, Ana C.; Valério-Fernandes, Ângela; Moura, Vera; Simões, Sérgio; Moreira, João Nuno. "Lipid-Based Nanosystems for the Delivery of siRNA: Challenges and Trends". In Handbook of Nanomaterials for Cancer Theranostics, 495-515. Elsevier, 2018.
    10.1016/b978-0-12-813339-2.00016-5
  3. Lopes, R.M.; Fonseca, N.A.; Cruz, A.F.; Gregório, A.C.; Valério-Fernandes, Â.; Moura, V.; Simões, S.; Moreira, J.N.. "Advances on nucleic acid delivery with nonviral vectors". In Bioinspired Materials for Medical Applications, 403-426. Elsevier, 2017.
    10.1016/b978-0-08-100741-9.00014-0
Conference abstract
  1. Chiara, Brignole; Bensa, Veronica; Zotto, Genny Del; Bruno, Silvia; Fonseca, Nuno A; Cruz, Ana F; Paolo, Daniela Di; et al. "Abstract A101: Nucleolin: A novel cell surface protein for neuroblastoma targeted therapy". Paper presented in AACR-NCI-EORTC International conference on Molecular Targets and Cancer Therapeutics, 2019.
    10.1158/1535-7163.targ-19-a101
  2. Cruz, Ana F.; Fonseca, Nuno A.; Gonçalves, Nélio; Moura, Vera; Simões, Sérgio; Moreira, João Nuno. "Abstract 3622: Differential effect of liposomal C6-ceramide/doxorubicin targeted to nucleolin and conventional combinations against triple negative breast cancer stem cells". Paper presented in American Association for Cancer Research Annual Meeting 2019, 2019.
    10.1158/1538-7445.am2019-3622
  3. Cruz, Ana Filipa; Fonseca, Nuno André; Sampaio, Susana F.; Moura, Vera; Almeida, Ramiro D.; Simões, Sérgio; Moreira, João Nuno. "Abstract 2194: Synergistic cytotoxicity of targeted liposomes containing doxorubicin and C6-ceramide against nucleolin-overexpressing ovarian cancer cells is supported by the downregulation of the Akt pathway". Paper presented in American Association for Cancer Research Annual Meeting 2017, 2017.
    10.1158/1538-7445.am2017-2194
Conference poster
  1. Cruz, Ana F; Fonseca, Nuno A; Moura, Vera; Simões, Sérgio; Moreira, JN. "P3.4: The potential of F3 peptide-targeted liposomes containing doxorubicin and C6-ceramide to imbalenced cancer stem cell niche in nucleolin-overexpressing cell lines". Paper presented in 3rd SPLC-CRS Young Scientists Virtual Meeting, 2021.
  2. Cruz, Ana Filipa; Gonçalves, Nélio; Moura, Vera; Simões, Sérgio; Moreira, João Nuno. "The potential of liposomal C6-ceramide/doxorubicin targeted to nucleolin to decrease triple-negative breast cancer stem cell population, relative to a standard of treatment.". Paper presented in 46th Controlled Release Society Annual Meeting & Exposition, 2019.
  3. Cruz, Ana F; Fonseca, Nuno A; Moura, Vera; Simões, Sérgio; Moreira, JN. "Cytotoxicity of targeted liposomal C6-ceramide and doxorubicin against nucleolin-overexpressing cancer cell lines is supported by the downregulation of the Akt pathway and is more effective than conventional drug combinations". Paper presented in XII Spanish-Portuguese Conference on Controlled Drug Delivery, 2018.
  4. Cruz, Ana F; Fonseca, Nuno A; Sampaio, Susana F; Moura, Vera; Almeida, Ramiro D.; Simões, Sérgio; Moreira, JN. "Unraveling the therapeutic potential of targeted synergistic drug combination against nucleolin-overexpressing ovarian cancer cell lines.". Paper presented in XI Spanish-Portuguese Conference on Controlled Drug Delivery, 2016.
  5. Cruz, Ana F; Fonseca, Nuno A; Moura, Vera; Simões, Sérgio; Moreira, JN. "Enhanced efficacy of targeted synergistic drug combinations against nucleolin-overexpressing ovarian cancer cell lines.". Paper presented in XIII CNC Annual Meeting, 2015.
Journal article
  1. Ângela Valério-Fernandes; Nuno A. Fonseca; Nélio Gonçalves; Ana F. Cruz; Marta I. Pereira; Ana C. Gregório; Vera Moura; et al. "Nucleolin Overexpression Predicts Patient Prognosis While Providing a Framework for Targeted Therapeutic Intervention in Lung Cancer". Cancers 14 9 (2022): 2217-2217. https://doi.org/10.3390/cancers14092217.
    10.3390/cancers14092217
  2. Cruz, Ana Filipa; Fonseca, Nuno A.; Malheiro, Ana Rita; B. Melo, Joana; Gaspar, Maria Manuela; Fernandes, Rui; Moura, Vera; Simões, Sérgio; Moreira, João Nuno. "Targeted liposomal doxorubicin/ceramides combinations: The importance to assess the nature of drug interaction beyond bulk tumor cells". European Journal of Pharmaceutics and Biopharmaceutics 172 (2022): 61-77. http://dx.doi.org/10.1016/j.ejpb.2022.01.006.
    10.1016/j.ejpb.2022.01.006
  3. Ana F. Cruz; Mariana B. Caleiras; Nuno A. Fonseca; Nélio Gonçalves; Vera M. Mendes; Susana F. Sampaio; Vera Moura; et al. "The Enhanced Efficacy of Intracellular Delivery of Doxorubicin/C6-Ceramide Combination Mediated by the F3 Peptide/Nucleolin System Is Supported by the Downregulation of the PI3K/Akt Pathway". Cancers 13 12 (2021): 3052-3052. https://doi.org/10.3390/cancers13123052.
    10.3390/cancers13123052
  4. Brignole, Chiara; Bensa, Veronica; Fonseca, Nuno A.; Del Zotto, Genny; Bruno, Silvia; Cruz, Ana F.; Malaguti, Fabiana; et al. "Cell surface Nucleolin represents a novel cellular target for neuroblastoma therapy". Journal of Experimental & Clinical Cancer Research 40 1 (2021): http://dx.doi.org/10.1186/s13046-021-01993-9.
    10.1186/s13046-021-01993-9
  5. Cruz, Ana Filipa; Fonseca, Nuno Andre; Moura, Vera; Simoes, Sergio; Moreira, Joao Nuno. "Targeting Cancer Stem Cells and Non-Stem Cancer Cells: The Potential of Lipid- Based Nanoparticles". Current Pharmaceutical Design 23 43 (2018): 6563-6572. http://dx.doi.org/10.2174/1381612823666171115105252.
    10.2174/1381612823666171115105252
  6. Fonseca, Nuno A.; Cruz, Ana Filipa; Moura, Vera; Simões, Sérgio; Moreira, João Nuno. "The cancer stem cell phenotype as a determinant factor of the heterotypic nature of breast tumors". Critical Reviews in Oncology/Hematology 113 (2017): 111-121. http://dx.doi.org/10.1016/j.critrevonc.2017.03.016.
    Published • 10.1016/j.critrevonc.2017.03.016

Intellectual property

Patent
  1. Cruz, Ana F; Moura, V; Fonseca N; Simões, S; Moreira, JN. 2020. "Overcoming drug resistance associated with cancer stem cells and methods thereof". Portugal.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/04/13 Differential effect of F3 peptide-targeted liposomes containing doxorubicin and ceramides and non-targeted drug combinations against nucleolin-overexpressing cancer cell lines. Advanced Therapies Course/Doctoral Program in Experimental Biology and Biomedicine (PDBEB)
Center for Neuroscience and Cell Biology/University of Coimbra (Coimbra, Portugal)
2022/01/08 The impact of different liposomal formulations on nucleolin-overexpressing cancer cell lines Módulo III:Nanotecnologias/Mestrado em Biotecnologia Farmacêutica
Faculty of Pharmacy/University of Coimbra (Portugal)
2021/04/28 Differential effect of targeted and non-targeted drug combinations against nucleolin-overexpressing cell lines. Curricular unit: Nanomedicamentos/Mestrado Integrado em Ciências Farmacêuticas
Faculty of Pharmacy, University of Lisbon
2021/04/22 Differential effect of F3 peptide-targeted liposomes containing doxorubicin and C6-ceramide and non-targeted drug combinations against nucleolin-overexpressing cancer cell lines. Advanced Therapies Course/Doctoral Program in Experimental Biology and Biomedicine (PDBEB)
Center for Neuroscience and Cell Biology/University of Coimbra (Coimbra, Portugal)
2020/11/17 Differential effect of targeted and non-targeted drug combinations against nucleolin-overexpressing cell lines Mestrado em Investigação Biomédica (MIB)
Faculty of Medicine, University of Coimbra (Coimbra, Portugal)
2019/11/15 Synergistic cytotoxicity of targeted liposomal C6-ceramide and doxorubicin against nucleolin-overexpressing cancer cells is supported by the downregulation of the PI3K/Akt pathway. 1st Annual Meeting in Pharmaceutical Sciences
Faculty of Pharmacy, University of Coimbra (Coimbra, Portugal)
2019/03/27 A new perspective about anti-cancer therapies. Project "Cientificamente Provável" established between "Centro de Inovação em Biomedicina e Biotecnologia da Universidade de Coimbra" and "Bibliotecas Escolares de Mira"
Escola Secundária com 3.ºCEB Dr. a Maria Cândida (Coimbra, Portugal)
2017/04/23 The enhanced efficacy of targeted liposomes containing doxorubicin and C6-ceramide against nucleolin-overexpressing cancer cell lines is supported by the downregulation of the Akt pathway. 1st SPLC-CRS Young Scientist Meeting
Spanish Portuguese Local Chapter of the Controlled Release Society (Santiago de Compostela, Spain)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2017/11/01 - 2018/01/16 As a member of the Young section of the SPLC-CRS, I contributed in the whole process of organization of the XII Spanish-Portuguese Conference on Controlled Drug Delivery: Tailoring drug delivery systems to the patients' needs. For example, I participated in the selection of venue and catering, organization of the poster area and carring microphones during discussion. (2018/01/14 - 2018/01/16)
Conference (Member of the Organising Committee)
 Universidade de Coimbra, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2021/10/14 - 2021/10/14 O FIC.A foi pensado para inovar, romper estaticismos e fundar uma nova realidade que cativasse quem, por algum motivo, se encontra afastado do mundo da ciência e da tecnologia. Uma verdadeira celebração da ciência em todas as suas vertentes, um festival de conhecimento, uma oportunidade para aprender, trocar experiências, estabelecer laços e inspirar públicos de todas as idades. https://www.fica.pt/
Festival
 Festival Internacional de Ciência, FICA 2021
Camara Municipal Oeiras, Portugal

Senciência, Portugal
2019/09/27 - 2019/09/27 European Researchers' Nights are public events dedicated to bringing researchers closer to the public. They showcase the diversity of research and highlight the impact of research on our daily lives. The aim is also to motivate young people to embark on research careers. The events promote how researchers contribute to our society by displaying their work in an interactive and engaging forum. https://ec.europa.eu/research/mariecurieactions/actions/european-researchers-night_en
Other
 European Researchers' Night
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019/03/01 - 2019/03/29 Brain Awareness Week (BAW) is a global campaign to increase public awareness of the progress and benefits of brain research promoted internationally by Federation of European Neuroscience Societies (FENS) and DANA Foundation and nationally by Ciência Viva and Sociedade Portuguesa de Neurociências. dana.org/BAW
Other
 Brain Awareness Week
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Association member

Society Organization name Role
2015 - Current Young Section of the Spanish-Portuguese Local Chapter of the Controlled Release Society
2012/07 - Current Portuguese Order of Pharmacists (OF)