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Leonor Silveira, PhD (Biology – Molecular Genetics; Universidade Nova de Lisboa - Faculdade de Ciências e Tecnologia), is a researcher at the National Reference Laboratory for Gastrointestinal Infections, at the National Health Laboratory Doutor Ricardo Jorge, Lisbon, Portugal. LSilveira has published 21 peer-reviewed papers, 11 papers in a national publication, over 30 congress abstracts, several reports, and a book chapter. LSilveira has collaborated in 12 funded research projects, including 5 One Health European Joint Programmes funded by the European Union’s Horizon 2020 research and innovation programme (grant agreement No 773830©2018). LSilveira is also ECDC’s Operational Focal Point for Salmonella and Vibrio cholerae and ECDC’s Focal Point for Salmonella antimicrobial resistance in Portugal, also participating in several ECDC’s expert groups. Additionally, LSilveira is responsible for the establishment and maintenance of the national laboratory and genomic surveillance for Salmonella, collaborating closely with several laboratories and the National Health Directorate. LSilveira has (co)-supervised over 18 students, including 10 MSc, 3 BSc, 5 Pharmaceutical Residencies, and many internships not conferring academic degree.
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Identification

Personal identification

Full name
Leonor de Sousa Dâmaso da Silveira

Citation names

  • Silveira, Leonor

Author identifiers

Ciência ID
3212-D6D9-D78B
ORCID iD
0000-0001-8485-3008
Google Scholar ID
https://scholar.google.com/citations?view_op=list_works&hl=pt-PT&user=Dt3yT6kAAAAJ
Scopus Author Id
57198777391

Email addresses

  • leonor.silveira@insa.min-saude.pt (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Medical and Health Sciences - Health Sciences - Infectious Diseases
  • Medical and Health Sciences - Health Sciences - Public and Environmental Health
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
French Beginner (A1) Intermediate (B1) Beginner (A1) Beginner (A1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2014/09 - 2019/12/18
Concluded
 Doutoramento em Biologia (Doutoramento)
Major in Genética Molecular
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Phenotypic and genotypic characterization of Salmonella spp. isolates in Portugal" (THESIS/DISSERTATION)
2011/03/21 - 2011/04/01
Concluded
 Creditação em Experimentação em Animais de Laboratório – Categoria C (FELASA) (Outros)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2011
Concluded
 Genética Molecular e Biomedicina (Mestrado)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Genetics and Molecular Mechanisms of Hypertrophic Cardiomyopathy: role of csrp3 and tcap genes" (THESIS/DISSERTATION)
 16
2008
Concluded
 Biologia Celular e Molecular (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"n/a" (THESIS/DISSERTATION)
2006 - 2007
Concluded
 Certificate of Proficiency in English (Outros)
University of Cambridge Faculty of English, United Kingdom
 B
2002 - 2003
Concluded
 Certificate in Advanced English (Outros)
University of Cambridge Faculty of English, United Kingdom
2001 - 2002
Concluded
 First Certificate in English (Outros)
University of Cambridge Faculty of English, United Kingdom
Affiliation

Science

Category
Host institution 		Employer
2012/03 - 2012/12 Research Trainee (Research) Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2009/09 - 2011/06 Research Trainee (Research) Universidade Lusófona de Humanidades e Tecnologias, Portugal

Other Careers

Category
Host institution 		Employer
2013/01/01 - Current Técnico Superior (Técnico Superior) Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Positions / Appointments

Category
Host institution 		Employer
2022/01 - Current Environmental, Health, and Safety Manager Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2014 - Current Responsible for Quality Management System of the National Reference Laboratory of Gastrointestinal Infections Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2013/01 - Current Responsible for Salmonella surveillance at the National Reference Laboratory for Gastrointestinal Infections Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2013/01 - Current Responsible for Vibrio cholerae surveillance at the National Reference Laboratory for Gastrointestinal Infections Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2020 - 2021 Member of the NIH Emergency Response to the Laboratory Diagnosis of COVID-19 in Portugal Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Others

Category
Host institution 		Employer
2015 - 2015 Trainer in Cooperative Mission in Guiné-Bissau Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
Institut National de la Santé, Guinea-Bissau
2010 - 2011 Tutor in Human Genetics Lab Classes Universidade Lusófona de Humanidades e Tecnologias, Portugal
Projects

Grant

Designation Funders
2025/09/01 - Current The Impact of Climate Change in the Transmission and Antimicrobial Resistance of Food- and Waterborne Bacterial Pathogens: An Integrated One Health Study in Lisbon and Tagus Valley
2025.07078.BDANA
Supervisor
Fundação para a Ciência e a Tecnologia
Ongoing

Contract

Designation Funders
2023 - Current Resistência aos antimicrobianos em isolados de Salmonella spp.– Importância em Saúde Pública
INSA
Principal investigator
Instituto Nacional de Saúde Doutor Ricardo Jorge
Ongoing
2023 - Current Avaliação da estrutura genómica de isolados clínicos de Salmonella Typhimurium e da sua variante monofásica
INSA
Principal investigator
Ongoing
2022 - Current A One Health study to understand the sources, transmission and persistence of foodborne zoonotic diseases.
FMVULusófona_FBZoonOH
Researcher
Universidade Lusófona de Humanidades e Tecnologias Faculdade de Medicina Veterinária, Portugal
Ongoing
2023 - 2025 Food safety risks from backyard animals raised for non-commercial purpose - a pilot study
FMVULusófona_Backyard
Researcher
Cooperativa de Formação e Animação Cultural CRL, Portugal
 Universidade Lusófona de Humanidades e Tecnologias Faculdade de Medicina Veterinária
Ongoing
2022/01 - 2024/12 Características genotípicas e fenotípicas que contribuem para a persistência de Listeria monocytogenes em ambiente de processamento alimentar
Provided by PTCRIS: PTDC/BAA-AGR/4194/2021
 Fundação para a Ciência e a Tecnologia
2021/03 - 2024/03 Profagos de Helicobacter: diversidade, evolução e papel na doença
Provided by PTCRIS: PTDC/BTM-TEC/3238/2020
 Fundação para a Ciência e a Tecnologia
2021 - 2024 Food- and Waterborne Diseases Antimicrobial Resistance - Reference Laboratory Capacity
FWD AMR-RefLabCap
Participante
Ongoing
2020/01/01 - 2022/06/30 OH-HARMONY-CAP: One Health Harmonisation of Protocols for the Detection of Foodborne Pathogens and AMR Determinants
JIP03-R2-IA2-OH-HARMONY CAP
Researcher
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
 European Commission
Concluded
2020/01/01 - 2022/06/30 MATRIX: Connecting dimensions in One-Health surveillance
JIP04-R2-IA2-MATRIX
Researcher
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
 European Commission
Concluded
2020/01/01 - 2022/06/30 DiSCoVer: Discovering the sources of Salmonella, Campylobacter, VTEC and antimicrobial resistance
JRP24-R2-FBZ2-DISCoVeR
Researcher
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
 European Commission
Concluded
2020/01/01 - 2022/06/30 ADONIS: Assessing Determinants of the Non-Decreasing Incidence of Salmonella
JRP26-R2-FBZ2-ADONIS
Researcher
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
 European Commission
Concluded
2020/01/01 - 2021/06/30 FED-AMR – The role of free extracellular DNA in dissemination of antimicrobial resistance over ecosystem boundaries along the food/feed chain
773830
Researcher
European Commission
Concluded
Outputs

Publications

Book chapter
  1. Silveira, Leonor; Machado, Jorge. "Salmonella spp. associada a roedores". In Doenças associadas a artrópodes vetores e roedores, 183-188. Portugal, 2019.
    Published
Conference poster
  1. Borges, Mariana; Martins, Margarida; Nunes, Alexandra; Ramos, Sónia; Santos, Isabel; Silva Camoesas, Mariana; Belas, Adriana; et al. "Characterisation Of Resistant Enteric Bacteria Isolated From Poultry Faeces and Meat In Portugal—Is There A Risk To Public Health?". Paper presented in The 4th International Electronic Conference on Antibiotics - Challenges and Strategies for the Antibiotic Resistance Crisis (online), 21–23 May, 2025.
  2. Balbino, Carolina; Pista, Ângela; Silveira, Leonor; Cordas, Matilde; Santos, Isabel; Silva, Mariana C.; Belas, Adriana; et al. Corresponding author: Nunes, Alexandra. "Investigation of multidrug-resistant enteric bacteria in pigs slaughtered for human consumption – Is there a risk for Public Health?". Paper presented in IAFP's European Symposium on Food Safety, Madrid, Spain, 6-8 May, 2025.
  3. Borges, Mariana; Martins, Margarida; Pista, Ângela; Silveira, Leonor; Cordas, Matilde; Santos, Isabel; Silva, Mariana C.; et al. "Assessing multidrug resistant enteric bacteria in poultry intended for human consumption: We must confront the public health risks head on!". Paper presented in IAFP's European Symposium on Food Safety, Madrid, Spain, 6-8 May, 2025.
  4. Smolders, Joaquim; Patrício, Rui; Valença, Andreia; Silveira, Leonor; Pista, Angela; Gomes, João Paulo; Nunes, Alexandra; Belas, Adriana. "ESBL/pAmpC-producing Enterobacteriaceae from healthy bearded dragons (Pogona Vitticepts): Preliminary results". 2025.
  5. Baldino, Carolina; Cordas, Matilde; Rosa, Rute; Santos, Isabel; Silva Camoesas, Mariana; Belas, Adriana; Alves, Margarida; et al. "Investigation of multidrug-resistant enteric bacteria in pigs produced for human consumption – Is there a risk for Public Health?". 2025.
  6. Carvalho, Miguel; Baldino, Carolina; Martins, Margarida; Borges, Mariana; Cordas, Matilde; Silveira, Leonor; Santos, Isabel; et al. "Evaluation of resistant enteric bacteria in raw meat from Portuguese retail stores". 2025.
  7. Roseiro, Isabel; Louro, Mónica; Silveira, Leonor; Oleastro, Mónica; Prof. Adriano O. Henriques; Serrano, M; Louro, Mónica; et al. "Characterization of the Clostridioides difficile biofilm matrix: Understanding the role of Glutamate Dehydrogenase". Paper presented in Evolving Microbes and Global Health Symposium, 2024.
  8. Campana, Sara; Fernandes, Gonçalo; Patrício, Rui; Alves, Margarida; Lecis, Roberta; Ropio, Joana; Valença, Andreia; et al. "Salmonella spp. serovars isolated from healthy Leopard geckos (Eublepharis macularius) in Lisbon, Portugal". 2024.
  9. Fernandes, Gonçalo; Patrício, Rui; Silveira, Leonor; Pista, Ângela; Gomes, João P.; Nunes, Alexandra; Belas, Adriana. "ZOONOTIC GRAM-NEGATIVE BACTERIA FROM THE ORAL CAVITY AND CLOACA OF HEALTHY LEOPARD GECKOS (EUBLEPHARIS MACULARIUS) - accepted and to be presented at the 6th International Conference on Avian, Herpetological, Exotic Mammal, Zoo and Wildlife Medicine, Ghent, Belgium, 20-24 May". 2024.
  10. Smolders, Joaquim; Patrício, Rui; Valença, Andreia; Silveira, Leonor; Pista, Ângela; Gomes, João P.; Nunes, Alexandra; Belas, Adriana. "ESBL/pAmpC-producing Enterobacteriaceae from healthy bearded dragons (Pogona Vitticepts): Preliminary results". Paper presented in FMVet Research Meetings 2024, Lisbon, Portugal, 05 July, 2024.
  11. Carvalho, Miguel; Balbino, Carolina; Martins, Margarida; Borges, Mariana; Cordas, Matilde; Silveira, Leonor; Santos, Isabel; et al. "Evaluation of resistant enteric bacteria in raw meat from Portuguese retail stores". Paper presented in FMVet Research Meetings 2024, Lisbon, Portugal, 05 July, 2024.
  12. Pista, Angela; Silveira, Leonor; C. Gonçalves; S. Costa; C. Carolino; B. Santo; Rute Rosa; et al. Corresponding author: Ramos, Sónia. "Are Food-Producing Animals a Source of Multidrug-resistant E. coli and Salmonella spp.?". 2023.
  13. Corrado Minetti; Silveira, Leonor; Pista Angela. "Antibiotic resistance profile of multidrug-resistant Shigella sonnei and Shigella flexneri in Portugal, 2015-2022". Paper presented in 33rd ECMID, 2023.
  14. Pista, Ângela; Silveira, Leonor; Gonçalves, Carlota; Costa, Sara; Carolino, Catarina; Santo, Bianca; Rosa, Rute; et al. Corresponding author: Nunes, Alexandra. "Are food-producing animals a source of multidrug-resistant E. coli and Salmonella spp.?". Paper presented in 6th International Congress of CiiEM - Immediate and Future Challenges to Foster One Health, Almada, Portugal, 5th-7th July, 2023.
  15. Campana, Sara; Fernandes, Gonçalo; Patrício, Rui; Alves, Margarida; Lecis, Roberta; Ropio, Joana; Valença, Andreia; et al. "Salmonella spp. serovars isolated from healthy Leopard geckos (Eublepharis macularius) in Lisbon, Portugal". Paper presented in FMVet Research Meetings 2023, Lisbon, Portugal, 19th May, 2023.
  16. Pista, Ângela; Silveira, Leonor; Costa, Sara; Rosa, Rute; Gonçalves, Carlota; Alves, Margarida; Belas, Adriana; et al. Corresponding author: Nunes, Alexandra. "Multidrug-resistant Escherichia coli and Salmonella spp. in pigs slaughtered for human consumption, a potential source for Humans? - published by MDPI in Proceedings of The 2nd International Electronic Conference on Microbiology (ECM 2023), 01-15 December". Paper presented in The 2nd International Electronic Conference on Microbiology (ECM 2023), 01-15 December, 2023.
  17. Fernandes, Gonçalo; Campana, Sara; Patrício, Rui; Silveira, Leonor; Pista, Ângela; Gomes, João P.; Nunes, Alexandra; Belas, Adriana. "Zoonotic Salmonella spp. isolated from healthy Leopard Geckos (Eublepharis Macularius): in vivo study". Paper presented in Montenegro International Veterinary Congress (XIX Edition), Santa Maria da Feira, Portugal, 13-14 October, 2023.
  18. Roseiro, Isabel; Louro, Mónica; Silveira, Leonor; Oleastro, Mónica; Adriano O. Henriques; Serrano, M. Corresponding author: Serrano, M. "Characterization of the Clostridioides difficile biofilm matrix". Paper presented in EMBO workshop Bacterial networks (BacNet22), 2022.
  19. Mónica Louro; Silveira, Leonor; Roseiro, Isabel; Adriano O. Henriques; Oleastro, Mónica; Serrano, M. Corresponding author: Serrano, M. "Clostridiodies difficile biofilms". Paper presented in SymbNET PhD Summer School on Host-Microbe Symbioses, 2022.
  20. Sofia Ribeiro; Mariana Fontes; Iúri Lopes; Silveira, Leonor; Ângela Pista. "Colistin resistance genes in Escherichia coli and Salmonella spp. isolates from pets, wild and food-producing animals in Portugal, 2019-2021". Paper presented in 32nd ECCMID, 2022.
  21. Ângela Pista; Sofia Ribeiro; Mariana Fontes; Iúri Lopes; Silveira, Leonor. "Phenotypic and genotypic characterization of Escherichia coli and Salmonella spp. in pets, Portugal 2019-2020 - A One Health perspective". Paper presented in OHEJP ASM 2022, 2022.
  22. Angela Pista; Sofia Ribeiro; Mariana Fontes; Iuri Lopes; Leonor Silveira. "Colistin resistance genes in Escherichia coli and Salmonella spp. in pets, wild and food-producing animals in Portugal". Paper presented in 1st Egas Moniz One Health Symposium, 2022.
  23. Roseiro, Isabel; Louro, Mónica; Silveira, Leonor; Henriques, Adriano; Oleastro, Mónica; Serrano, M. "Identification of Clostridioides difficile biofilm matrix proteins". Paper presented in MicroBiotec21, 2021.
  24. Castro, Rita; Alves, Frederico; Silveira, Leonor; Nunes, Alexandra; Oleastro, Mónica. "Diversity and dynamics of Clostridioides difficile in a farm environment". Paper presented in One Health EJP Annual Scientific Meeting 2021, 2021.
  25. Silveira, Leonor; Ribeiro, Sofia; Lopes, Iuri; Fontes, Mariana; Castro, Rita; Alves, Frederico; Pista, Ângela. "Prevalence and characterization of salmonella spp. and pathogenic Escherichia coli in food-producing animals in Portugal". Paper presented in One Health EJP Annual Scientific Meeting 2021, 2021.
  26. Ângela Pista; Sofia Ribeiro; Mariana Fontes; Iúri Lopes; Rita Castro; Leonor Silveira. "Detection and Characterization of Enteropathogenic Escherichia coli and Salmonella spp. in Cats and Dogs from a Lisbon Area Kennel - A ONE HEALTH Perspective". 2021.
  27. Angela Pista; Sofia Ribeiro; Mariana Fontes; Rita Batista; Anabela Coelho; Silveira, Leonor; et al. "Prevalence and characterization of pathogenic Escherichia coli and Salmonella spp. in wild animals in mainland Portugal". Paper presented in OHEJP ASM 2021, 2021.
  28. Silveira, Leonor; Pinto, Miguel; Isidro, Joana; Pista, Ângela; Themudo, Patrícia; Vieira, Luís; Machado, Jorge; Gomes, João Paulo. "WGS for cluster detection and characterization of Salmonella Rissen isolates from Portugal". Paper presented in 12th International Meeting on Microbial Epidemiological Markers (IMMEM XII), 2019.
  29. Silveira, Leonor; Miguel Pinto; Joana Isidro; Ângela Pista; Patrícia Themudo; Luís Vieira; Jorge Machado; João Paulo Gomes. "Whole Genome Sequencing for cluster detection and characterization of Salmonella Rissen isolates from Portugal". Paper presented in 12th International Meeting on Microbial Epidemiological Markers (IMMEM XII), 2019.
  30. Silveira, Leonor; Pista, Ângela; Borges, Vítor; Silva, Catarina; Vieira, Luís; Gomes, João Paulo; Machado, Jorge. "The use of molecular typing for characterization of a Salmonella Enteritidis cluster". 2018.
  31. Silveira, Leonor; Pista, Angela; Machado, Jorge. "Salmonella enterica: serotipos e resistência aos antimicrobianos de isolados clínicos entre 2014 e 2017". Paper presented in 11as Jornadas de Atualização em Doenças Infeciosas do Hospital de Curry Cabral, 2018.
  32. Silveira, Leonor; Pista, Angela; Machado, Jorge. "Serotype distribution and antimicrobial resistance of clinical Salmonella enterica isolated in Portugal between 2014 and 2017". Paper presented in 28th ECCMID, 2018.
  33. Campos, Joana; Mourão, Joana; Silveira, Leonor; Saraiva, Margarida; Belo Correia, Cristina; Maçãs, Ana Paula; Peixe, Luísa Vieira; Antunes, Patrícia. "Carne de aves importada como fonte de transmissão de Salmonella Heidelberg e S. Minnesota resistentes a cefalosporinas de espetro alargado". Paper presented in 10th Annual Meeting PortFIR, 2017.
  34. Campos, Joana; Mourão, Joana; Silveira, Leonor; Saraiva, Margarida; Belo Correia, Cristina; Maças, Ana Paula; Peixe, Luísa Vieira; Antunes, Patrícia. "Imported Poutry Meat Products in Portugal as a source of Extended-Spectrum Cephalosporin-Resistant Salmonella Heidelberg CYM-2-Producing". Paper presented in 7th FEMS Congress of European Microbiologists, 2017.
  35. "Extended-Spectrum Cephalosporin-Resistant CMY-2-Producing Salmonella Heidelberg and S. Minnesota in Poultry Meat Imported into the European Union". 2017.
  36. "Carne de aves importada como fonte de transmissão de Salmonella Heidelberg e S. Minnesota resistentes a cefalosporinas de espectro alargado". 2017.
  37. "Caracterização molecular e suscetibilidade aos antimicrobianos de isolados de L. monocytogenes na região de LVT em 2015". 2017.
  38. Campos, Joana; Cristino, Luís; Giro, A.; Cunha, M.; Mourão, Joana; Silveira, Leonor; Machado, Jorge; Peixe, Luísa Vieira; Antunes, Patrícia. "Resistance to clinically relevant antibiotics in emerging Salmonella serotypes from food products in Portugal". Paper presented in Congresso Internacional de Nutrição e Segurança Alimentar - CINSA, 2017.
  39. Campos, Joana; Mourão, Joana; Silveira, Leonor; Saraiva, Margarida; Belo Correia, Cristina; Maçãs, Ana Paula; Peixe, Luísa; Antunes, Patrícia. "Carne de aves importada como fonte de transmissão de Salmonella Heidelberg e S. Minnesota resistentes a cefalosporinas de espectro alargado". 2017.
  40. Antunes, Patrícia; Campos, Joana; Mourão, Joana; Silveira, Leonor; Machado, Jorge; Novais, Carla; Peixe, Luísa Vieira. "Extended-spectrum cephalosporin resistance and plasmid-mediated quinolone resistance in emerging Salmonella serotypes from traded food products in Portugal". Paper presented in 26th ECCMID European Congress of Clinical Microbiology and Infectious Diseases, 2016.
  41. Campos, Joana; Mourão, Joana; Silveira, Leonor; Machado, Jorge; Novais, Carla; Peixe, Luísa Vieira; Antunes, Patrícia. "Clonal dynamics of diverse Salmonella enterica serotypes from traded food products in Portugal". Paper presented in 11th International Meeting on Microbial Epidemiological Markers (IMMEM), 2016.
  42. "Microevolution and persistence of Listeria monocytogenes in the environment and links to a foodborne outbreak". 2016.
  43. Borges, Vítor; Maia, Carla; Barreira, Maria João; Silveira, Leonor; Silva, Catarina; Duarte, Sílvia; Vieira, Luís; et al. "Microevolution and persistence of Listeria monocytogenes in the environment and links to a foodborne outbreak". 2016.
  44. Campos, Joana; Mourão, Joana; Silveira, Leonor; Machado, Jorge; Novais, Carla; Luisa Peixe; Antunes, Patrícia. "Extended-spectrum cephalosporin resistance and plasmid-mediated quinolone resistance in emerging Salmonella serotypes from traded food products in Portugal". 2016.
  45. Campos, Joana; Mourão, Joana; Silveira, Leonor; Machado, Jorge; Novais, Carla; Luisa Peixe; Antunes, Patrícia. "Clonal dynamics of diverse salmonella enterica serotypes from traded food products in Portugal". 2016.
Journal article
  1. Gijs Teunis; Timothy J. Dallman; Magdalena Zajac; Magdalena Skarzynska; Liljana Petrovska; Angela Pista; Leonor Silveira; et al. "Attributable sources of the five most prevalent non-typhoidal Salmonella serovars across ten European countries". Journal of Infection (2025): https://doi.org/10.1016/j.jinf.2025.106632.
    10.1016/j.jinf.2025.106632
  2. Inês C. Rodrigues; Marisa Ribeiro-Almeida; Joana Campos; Leonor Silveira; Liliana Leite-Martins; Jorge Ribeiro; Paula Martins da Costa; et al. "Uropathogenic Escherichia coli in a Diabetic Dog with Recurrent UTIs: Genomic Insights and the Impact of Glucose and Antibiotics on Biofilm Formation". Microorganisms (2025): https://doi.org/10.3390/microorganisms13081946.
    10.3390/microorganisms13081946
  3. Camille Jacqueline; Corrado Minetti; Sara Monzon Fernandez; Leonor Silveira; Isabel Cuesta De La Plaza; Ângela Pista; Silvia Herrera-Leon. "Genomic epidemiology of fluoroquinolone-resistant strains of Shigella sonnei and Shigella flexneri in the Iberian Peninsula from 2015 to 2022". The Journal of Infectious Diseases (2024): https://doi.org/10.1093/infdis/jiae596.
    10.1093/infdis/jiae596
  4. Carlota Gonçalves; Leonor Silveira; João Rodrigues; Rosália Furtado; Sónia Ramos; Alexandra Nunes; Ângela Pista; et al. Corresponding author: Nunes, Alexandra. "Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella spp. Isolates from Pigs at Slaughterhouse and from Commercial Pork Meat in Portugal". Antibiotics (2024): https://doi.org/10.3390/antibiotics13100957.
    Submitted • 10.3390/antibiotics13100957
  5. McGeoch, Luke J.; Hoban, Ann; Sawyer, Clare; Rabie, Hussein; Painset, Anaïs; Browning, Lynda; Brown, Derek; et al. "Salmonella Saintpaul outbreak associated with cantaloupe consumption, United Kingdom and Portugal, September 2023 to November 2023". Epidemiology and Infection (2024): 1-15. http://dx.doi.org/10.1017/s0950268824000670.
    10.1017/s0950268824000670
  6. Inês C. Rodrigues; Marisa Ribeiro-Almeida; Leonor Silveira; Joana C. Prata; André Pinto de Carvalho; Carla Roque; João Paulo Gomes; et al. "Unveiling a Listeria monocytogenes Outbreak in a Rabbit Farm: Clinical Manifestation, Antimicrobial Resistance, Genomic Insights and Environmental Investigation". Microorganisms (2024): https://doi.org/10.3390/microorganisms12040785.
    10.3390/microorganisms12040785
  7. Célia Leão; Leonor Silveira; Ana Usié; Joana Gião; Lurdes Clemente; Patricia Themudo; Ana Amaro; Angela Pista. "Genetic Diversity of Salmonella enterica subsp. enterica Serovar Enteritidis from Human and Non-Human Sources in Portugal". Pathogens (2024): https://doi.org/10.3390/pathogens13020112.
    10.3390/pathogens13020112
  8. Camille Jacqueline; Corrado Minetti; Silveira, Leonor; Angela Pista; Silvia Leon. "Genomic epidemiology of fluoroquinolone-resistant strains of Shigella sonnei and Shigella flexneri in the Iberian Peninsula from 2015 to 2022". (2024):
    Submitted
  9. Inês do Couto Rodrigues; Ana Paula Cristal; Marisa Ribeiro-Almeida; Leonor Silveira; Joana C Prata; Roméo Simões; Paulo Vaz-Pires; Angela Pista; Pista Angela; A Pista; Pista A; Â Pista; Pista Â; Ângela Pista; Paulo Martins da Costa. "Gulls in Porto Coastline as Reservoirs for Salmonella spp.: Findings from 2008 and 2023". Microorganisms (2023): http://dx.doi.org/10.3390/microorganisms12010059.
    10.3390/microorganisms12010059
  10. Leonor Silveira; Ângela Pista. "First Report of Salmonella Serovar Typhimurium and Monophasic Typhimurium Clinical Isolates Harboring mcr-9 in Portugal". Acta Médica Portuguesa (2023): https://doi.org/10.20344/amp.20111.
    10.20344/amp.20111
  11. Inês do Couto Rodrigues; Marisa Ribeiro-Almeida; Ribeiro J.; Leonor Silveira; Joana C Prata; Angela Pista; Pista Angela; A Pista; Pista A; Â Pista; Pista Â; Ângela Pista; Paulo Martins da Costa. "Occurrence of Multidrug-Resistant Bacteria Resulting from the Selective Pressure of Antibiotics: A Comprehensive Analysis of ESBL K. pneumoniae and MRSP Isolated in a Dog with Rhinorrhea". Veterinary Sciences 10 5 (2023): 326-326. http://dx.doi.org/10.3390/vetsci10050326.
    10.3390/vetsci10050326
  12. Sara Perestrelo; Ana Amaro; Michael S. M. Brouwer; Maria de Lurdes Tavares Clemente; Ana Sofia Ribeiro Duarte; Annemarie Kaesbohrer; Renata Karpíšková; et al. "Building an International One Health Strain Level Database to Characterise the Epidemiology of AMR Threats: ESBL—AmpC Producing E. coli as An Example—Challenges and Perspectives". Antibiotics 12 3 (2023): 552-552. http://dx.doi.org/10.3390/antibiotics12030552.
    10.3390/antibiotics12030552
  13. Alves, Frederico; Castro, Rita; Pinto, Miguel; Nunes, Alexandra; Pomba, Constança; Oliveira, Manuela; Silveira, Leonor; et al. "Molecular epidemiology of Clostridioides difficile in companion animals: Genetic overlap with human strains and public health concerns". Frontiers in Public Health 10 (2023): http://dx.doi.org/10.3389/fpubh.2022.1070258.
    10.3389/fpubh.2022.1070258
  14. Batista, Rita; Saraiva, Margarida; Lopes, Teresa; Silveira, Leonor; Coelho, Anabela; Furtado, Rosália; Castro, Rita; et al. "Caracterização genotípica e fenotípica de estirpes de Escherichia coli patogénicas, Salmonella spp. e Campylobacter spp. isoladas de aves em liberdade em Portugal continental". (2023): http://hdl.handle.net/10400.18/8886.
  15. Pista, Ângela; Silveira, Leonor; Ribeiro, Sofia; Fontes, Mariana; Castro, Rita; Coelho, Anabela; Furtado, Rosália; et al. "Escherichia coli patogénica, Salmonella spp. e Campylobacter spp. em dois Centros de Conservação da Vida Selvagem em Portugal: caracterização genotípica e fenotípica". (2023): http://hdl.handle.net/10400.18/8885.
  16. "Assessment of the Transmission Dynamics of Clostridioides difficile in a Farm Environment Reveals the Presence of a New Toxigenic Strain Connected to Swine Production". Frontiers in Microbiology 13 (2022): http://dx.doi.org/10.3389/fmicb.2022.858310.
    10.3389/fmicb.2022.858310
  17. Moura-Alves, Márcio; Carvalho, Marta; Baggio Ribeiro, Deise Helena; Barbosa, Joana; Silveira, Leonor; Pista, Ângela; Pinto, Helena Patrícia; et al. "Hygiene indicators and Salmonella sp. on swine carcass surfaces from two slaughterhouses in northern Portugal.". Journal of Food Protection (2022): http://dx.doi.org/10.4315/jfp-21-312.
    10.4315/jfp-21-312
  18. Borges, Vítor; Isidro, Joana; Trovão, Nídia Sequeira; Duarte, Sílvia; Cortes-Martins, Helena; Martiniano, Hugo; Gordo, Isabel; et al. "SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal". Communications Medicine 2 1 (2022): http://dx.doi.org/10.1038/s43856-022-00072-0.
    10.1038/s43856-022-00072-0
  19. Pista, Angela; Silveira, Leonor; Ribeiro, Sofia; Fontes, Mariana; Castro, Rita; Coelho, Anabela; Furtado, Rosália; et al. "Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization". Microorganisms 10 11 (2022): 2132-2132. http://hdl.handle.net/10400.18/8430.
    10.3390/microorganisms10112132
  20. Batista, Rita; Saraiva, Margarida; Lopes, Teresa; Silveira, Leonor; Coelho, Anabela; Furtado, Rosália; Castro, Rita; et al. "Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal". International Journal of Environmental Research and Public Health 20 1 (2022): 223-223. http://hdl.handle.net/10400.18/8514.
    10.3390/ijerph20010223
  21. Borges, Vítor; Isidro, Joana; Cortes-Martins, Helena; Duarte, Sílvia; Vieira, Luís; Leite, Ricardo; Gordo, Isabel; et al. "Massive dissemination of a SARS-CoV-2 Spike Y839 variant in Portugal". Emerging Microbes & Infections 9 1 (2020): 2488-2496. http://dx.doi.org/10.1080/22221751.2020.1844552.
    10.1080/22221751.2020.1844552
  22. Equipas de resposta à emergência do diagnóstico laboratorial da COVID-19 no INSA. "INSA and the emergency response to the laboratory diagnosis of COVID-19 in Portugal / O INSA e a resposta de emergência ao diagnóstico laboratorial da COVID-19 em Portugal". Boletim Epidemiológico Observações 9 Supl 12 (2020): 9-18.
  23. Silveira, Leonor; Nunes, Alexandra; Pista, Ângela; Isidro, Joana; Belo Correia, Cristina; Saraiva, Margarida; Batista, Rita; et al. "Characterization of multidrug-resistant isolates of Salmonella enterica serovars Heidelberg and Minnesota from fresh poultry meat imported to Portugal". Microbial Drug Resistance (2020):
    Accepted
  24. Campos, Joana; Mourão, Joana; Silveira, Leonor; Saraiva, Margarida; Correia, Cristina Belo; Maçãs, Ana Paula; Peixe, Luísa; Antunes, Patrícia. "Imported poultry meat as a source of extended-spectrum cephalosporin-resistant CMY-2-producing Salmonella Heidelberg and Salmonella Minnesota in the European Union, 2014–2015". International Journal of Antimicrobial Agents 51 1 (2018): 151-154. http://dx.doi.org/10.1016/j.ijantimicag.2017.09.006.
    10.1016/j.ijantimicag.2017.09.006
  25. Silveira, Leonor; Pista, Ângela; Machado, Jorge; Marques, Adelaide; Leonor, Silveira; Nunes, Alexandra; Isidro, Joana; et al. "Caracterização fenotípica de isolados de Shigella spp. entre 2015 e 2017". Microbial Drug Resistance 27 1 (2018): 87-98. http://hdl.handle.net/10400.18/5550.
    10.1089/mdr.2019.0384
  26. Silveira, Leonor; Pista, Ângela; Maia, Carla; Barreira, Maria João; Rodrigues, João; Reis, Lúcia; Machado, Jorge. "Caraterização molecular e suscetibilidade aos antimicrobianos de isolados clínicos de Listeria monocytogenes na região de Lisboa e Vale do Tejo em 2015". Boletim Epidemiológico Observações (2016): http://hdl.handle.net/10400.18/4107.
  27. Viegas, Silvia; Cunha, Isabel Campos; Correia, Cristina Belo; Coelho, Anabela; Maia, Carla; Pena, Cláudia; Bonito, Conceição Costa; et al. "Investigação laboratorial de surtos de toxinfeções alimentares, 2015". Boletim Epidemiológico Observações (2016): http://hdl.handle.net/10400.18/4130.
  28. "Sequenciação de Nova Geração no apoio à decisão de transplante pulmonar num doente com fibrose quística". Boletim Epidemiológico Observações (2015):
  29. "Perfis de resistência de Salmonella Enteritidis, Salmonella Typhimurium e Salmonella 4,5,[12]:i:- responsáveis por infeção humana, 1998-2013". Boletim Epidemiológico Observações (2014):
  30. "Salmonella enterica: serotipos menos frequentes com importância em patologia humana, caraterizados no INSA entre 2002-2013". Boletim Epidemiológico Observações (2014):
  31. Silveira, Leonor; Marques, Adelaide; Machado, Jorge. "Patotipos de Escherichia coli associados a infeções entéricas entre 2002-2012". (2013): http://hdl.handle.net/10400.18/1678.
  32. Santos, Susana; Marques, Vanda; Pires, Marina; Silveira, Leonor; Oliveira, Helena; Lança, Vasco; Brito, Dulce; et al. "High resolution melting: improvements in the genetic diagnosis of hypertrophic cardiomyopathy in a Portuguese cohort". BMC Medical Genetics 13 1 (2012): http://dx.doi.org/10.1186/1471-2350-13-17.
    10.1186/1471-2350-13-17
  33. Silveira, Leonor; Santos, Susana; Lança, Vasco; Oliveira, Helena; Branco, Patrícia; Silveira, Leonor; Marques, Vanda; et al. "Genetic diagnosis of hypertrophic cardiomyopathy using mass spectrometry DNA arrays and high resolution melting.". Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology 30 1 (2011): 7-18. http://hdl.handle.net/10451/34824.
    Published
Report
  1. Sofia Núncio; Filipe Duarte Santos; Luis Dias; Elisabete Valério; Angela Pista; Silveira, Leonor; Rita Sousa; Oleastro, Mónica. 2025. Relatório Saúde e Ambiente 2024. https://cpsa.pt/relatorio-observatorio-2024/.
  2. Declan Bolton; et al. 2022. Data management & harmonised reporting of Shiga toxin-producing Escherichia coli (STEC), Enterotoxigenic Escherichia coli (ETEC), Cryptosporidium and antimicrobial resistance (AMR) in Salmonella and Campylobacter spp. in the European Union. https://zenodo.org/records/6899935.
  3. Flemming Scheutz; et al. 2021. Deliverable D-IA.2.2.OH-Harmony-Cap.2.2 Results and Evaluation of the Pilot Survey.
  4. Declan Bolton; et al. 2020. Deliverable D-IA.2.2.OH-Harmony-Cap.3.1, Technical Report: Sampling and testing of Shiga toxin-producing Escherichia coli (STEC), Enterotoxigenic Escherichia coli (ETEC), Cryptosporidium and antimicrobial resistance (AMR) in Salmonella and Campylobacter spp. in the European Union. https://zenodo.org/records/4381374#.YiPmS6vP2Uk.
Thesis / Dissertation
  1. "O Frango como fonte de bactérias zoonóticas multirresistentes para o Homem". Master, Universidade Nova de Lisboa, 2024.
  2. Lopes, Iúri Miguel Quintanilha Branquinho. "Laboratory surveillance of Salmonella enterica in Portugal, 2020". Master, 2021. http://hdl.handle.net/10451/51212.
  3. Silveira, Leonor de Sousa Dâmaso da; Silveira, Leonor. "Phenotypic and genotypic characterization of Salmonella spp. isolates in Portugal". PhD, 2019. http://hdl.handle.net/10362/91582.
  4. Silveira, Leonor de Sousa Dâmaso da. "Genética e mecanismos moleculares na miocardiopatia hipertrófica: papel dos genes CSRP3 e TCAP". Master, 2011. http://hdl.handle.net/10362/7035.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/11/28 Caracterização molecular de Escherichia coli (E. coli) - Um olhar sobre a diversidade e sobre as implicações para a clínica e para a Saúde Pública I Workshop Diagnóstico in vitro LusoPalex
(Porto, Portugal)
2014/05/21 Perfis de resistência de Salmonella spp. em infeções humanas Antibióticos na cadeia alimentar: um risco para a Saúde humana?
INSA

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023 - Current Estrutura genómica de isolados clínicos dos serotipos de Salmonella mais frequentes em Portugal
Supervisor
 Mestrado em Biologia Humana e Ambiente (Master)
Universidade de Lisboa, Portugal
2023 - 2024 Resistência aos antimicrobianos em isolados de Salmonella spp.– Importância em Saúde Pública
Supervisor
 Mestrado em Biologia Humana e Ambiente
Universidade de Lisboa, Portugal
2023 - 2024 O frango como fonte de bactérias zoonóticas multirresistentes para o Homem
Supervisor
 Mestrado em Genética Molecular e Biomedicina (Master)
Universidade Nova de Lisboa, Portugal
2023 - 2024 Yersinia enterocolitica e Shigella spp. em animais para consumo humano - Qual o risco para a Saúde Pública?
Co-supervisor
 Mestrado em Microbiologia Aplicada (Master)
Universidade de Lisboa, Portugal
2023 - 2024 Pesquisa de bactérias entéricas multirresistentes em perus produzidos para consumo humano - Existe risco para a Saúde Pública?
Co-supervisor
 Mestrado em Biologia Humana e Ambiente (Master)
Universidade de Lisboa, Portugal
2023 - 2024 Caracterização genotípica e fenotípica de estirpes invasivas de Salmonella spp., E. coli, Shigella spp. e Yersinia enterocolitica
Co-supervisor
 Mestrado em Biologia Humana e Ambiente (Master)
Universidade de Lisboa, Portugal
2023 - 2023 Multidrug resistant Salmonella spp. and E.coli in Portugal: a OneHealth study to understand the sources, transmission and persistence, with a focus on turkeys
Supervisor
 Licenciatura em Bioquímica (Degree)
Universidade Lusófona de Humanidades e Tecnologias, Portugal
2020 - 2021 Laboratory surveillance of Salmonella enterica in Portugal, 2020
Supervisor
 Mestrado em Microbiologia Aplicada (Master)
Universidade de Lisboa, Portugal
2015 - 2016 Salmonella Typhimurium and monophasic serovar 4,[5],12:i:-
Supervisor
 Mestrado de Microbiologia Aplicada (Other)
Universidade de Lisboa, Portugal
2014 - 2014 Tipagem de Salmonella
Supervisor

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2024/12 O frango como fonte de bactérias zoonóticas multirresistentes para o homem.
Supervisor
 Mariana Mixão Borges (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2021 Laboratory surveillance of Salmonella enterica in Portugal, 2020
Supervisor
 Iúri Miguel Branquinho (Master)
Universidade de Lisboa, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2025/01 - Current International Journal of Food Microbiology
2024 - 2024 Scientific Reports Nature
2023 - 2023 International journal of food microbiology Elsevier

Consulting

Activity description Institution / Organization
2025/10 - Current ECDC's Vibriosis surveillance working group
2025/08/22 - Current ESCMID Study Group for Epidemiological Markers (ESGEM-AMR)- E. coli/Shigella, Salmonella subgroup
2014 - Current Portuguese expert in Salmonella antimicrobial resistance in the European Food- and Waterborne Diseases and Zoonoses Network (FWD-NET) European Centre for Disease Prevention and Control, Sweden
2015 - 2016 Development of operational procedures for FWD molecular surveillance and cluster response (FWD CRESP) European Centre for Disease Prevention and Control, Sweden

Mentoring / Tutoring

Topic Student name
2024/05/01 - Current Formação de alunos de Residência Farmacêutica Alunos de Residência Farmacêutica
2016 - Current Vigilância de bactérias entéricas Estagiários de internato em Infecciologia

Other jury / evaluation

Activity description Institution / Organization
2025 - Current Júri do Período Experimental da Técnica Superior Cristina Maria de Oliveira Matias Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2024 - Current Caracterização fenotípica e genotípica de estirpes de Clostridioides difficile de origem clínica Universidade Lusófona de Humanidades e Tecnologias Escola de Psicologia e Ciências da Vida, Portugal