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Ana Raquel Coelho graduated in Clinical Physiology at Coimbra Health School in 2012. During the curricular internship year, she worked at Coimbra University Hospital and 3 months at Agostino Gemelli University Policlinic (Rome). Afterwards, she obtained her MSc degree in Cellular and Molecular Biology from University of Coimbra, and, in 2020, she completed her PhD in Experimental Biology and Biomedicine at the same University, under a FCT PhD individual fellowship. Her PhD project was developed at the Institute of Biotechnology of the Czech Academy of Sciences, where she spent 3 years, and in the Center of Neuroscience and Cell Biology in Coimbra, under supervision of Prof. Jiri Neuzil and Dr. Paulo Oliveira. During master, she developed a project focused on the molecular and cellular protective mechanisms of Sirtuin 3 against the toxicity induced by the chemotherapeutic doxorubicin in cardiomyocytes. During PhD, Ana Raquel Coelho has made significant contributions to the understanding of the importance of mitochondrial metabolism in cancer. She provided evidence of mitochondrial trafficking between mammalian cells in vivo, and consequently, the essential role of mitochondrial respiration for tumor initiation and progression. In 2018, supported by a FLAD Healthcare 2020 project, she collaborated with Prof. Pinchas Cohen, spending 3 months in his laboratory in the University of Southern California, LA, USA. The scientific objective was to identify the role of the mtDNA-encoded peptide MOTS-c as a critical mediator of the beneficial effects of physical activity in skeletal muscle. She has also been collaborating in a project focus on the development of mitochondrial-targeted antioxidants, both from natural and semi-synthetic origin, as a therapeutic approach for aging-associated diseases. In 2021, she joined Gonçalo Bernardes' Lab, at IMM, emerging in the field of chemical bioconjugation and chemical biology. After working in several projects related with SARS-CoV-2, she is currently working on targeted-anti-cancer therapies, by developing and testing miniprotein-drug conjugates. Briefly, she has published 1 book chapter and 12 publication in peer-reviewed international journals (10 at Q1, and 2 Top-5% journals), 4 as first author, mostly in the field of cancer biology. Overall, she has a total of approximately 500 citations and a 9 h-index. Ana Raquel Coelho participated in 7 international events and was on the organizing committee of 3 scientific meetings. She enthusiastically promotes knowledge transfer by teaching and mentoring students, lecturing on cancer metabolism classes in MSc and PhD degrees at the University of Coimbra and participating in outreach science activities for society.
Identification

Personal identification

Full name
Ana Raquel Ligeiro Coelho

Citation names

  • Coelho, AR
  • Coelho, Ana

Author identifiers

Ciência ID
2314-E49D-3FF9
ORCID iD
0000-0002-0965-7573

Knowledge fields

  • Natural sciences - Biological Sciences - Cell Biology
  • Medical and Health Sciences - Other Medical Sciences
  • Natural sciences - Biological Sciences - Molecular Biology
  • Medical and Health Sciences - Basic Medicine - Medicinal Chemistry
  • Medical and Health Sciences - Basic Medicine - Toxicology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Italian Intermediate (B1) Beginner (A1) Beginner (A1) Intermediate (B1)
English Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2)
Education
Degree Classification
2015 - 2020
Concluded
Experimental Biology and Biomedicine (Doutoramento)
Universidade de Coimbra, Portugal
"Molecular mechanism of mitochondrial transfer from stromal cells to cancer cells with damaged mitochondrial DNA" (THESIS/DISSERTATION)
Honours and Distinction
2012 - 2014
Concluded
Cellular and Molecular Biology (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Role of Sirtuin 3 on Doxorubicin-induced Toxicity on H9c2 Cardiomyoblasts" (THESIS/DISSERTATION)
18
2008 - 2012
Concluded
Clinical Physiology (Licenciatura)
Instituto Politécnico de Coimbra Escola Superior de Tecnologia da Saúde de Coimbra, Portugal
"Influência da Ingestão de Álcool no Sistema Cardiorespiratório" (THESIS/DISSERTATION)
16
Affiliation

Science

Category
Host institution
Employer
2021/03 - Current Contracted Researcher (Research) Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
2020/07 - 2021/02 Contracted Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2014/10 - 2015/04 Visiting Researcher (Research) Biotechnologický ústav Akademie ved Ceské republiky, Czech Republic

Other Careers

Category
Host institution
Employer
2019/09 - 2020/07 Técnico Superior (Técnico Superior) Portuguese Green-Up, Portugal
2012/05 - 2012/09 Técnico Principal (Técnico de Diagnóstico e Terapêutica - Técnico de Cardiopneumologia) Clinicentro - Policlínica de Coimbra, Portugal

Others

Category
Host institution
Employer
2018/05 - 2018/09 Visiting PhD student University of Southern California Leonard Davis School of Gerontology, United States
2015/09 - 2018/04 PhD student Biotechnologický ústav Akademie ved Ceské republiky, Czech Republic
2011/09 - 2012/06 Curricular Training - Cardiovascular and respiratory study, Sleep study and polysomnography Centro Hospitalar e Universitário de Coimbra EPE, Portugal
2012/01 - 2012/04 Curricular Training under the Erasmus Programme - Hemodynamic, electrophysiology and pacing, cardiovascular perfusion Policlinico Universitario Agostino Gemelli, Italy
Università Cattolica del Sacro Cuore Sede di Roma, Italy
Projects

Grant

Designation Funders
2022/05/01 - Current A Bispecific antibody to block iron-endocytosis-driven metastasis
EXPL/MED-QUI/0232/2021
Researcher
Fundação para a Ciência e a Tecnologia
2021/03/01 - Current Intracellular Drug Delivery using Small Molecule Drug Conjugates for Prostate Cancer Therapy
PTDC/MED-QUI/28764/2017
Researcher
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
Fundação para a Ciência e a Tecnologia
2017 - 2019 The role of mitochondrial respiration in tumour initiation and growth: A mechanistic study
17-01192J
PhD Student Fellow
Grantová agentura Ceské republiky
2015 - 2017 Acquisition of the mitochondrial genome by cells with damaged mitochondrial DNA restores the mitochondrial function and tumorigenic capacity
15-02203S
PhD Student Fellow
Grantová agentura Ceské republiky
2012 - 2014 Protective Activity of SIRT3 Against Drug-induced Mitochondrial Dysfunction
PTDC/SAU-TOX/110952/2009
Other
Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2020/07 - 2021 SkinShield - Redox-active phytochemicals triggering mitochondrial hormesis: developing a new generation of cosmetic active ingredients
POCI-01-0145-FEDER-028607
Researcher
Fundação para a Ciência e a Tecnologia
Outputs

Publications

Book chapter
  1. Ferreira, L.L.; Coelho, A.R.; Oliveira, P.J.; Cunha-Oliveira, T.. "Mitochondrial Toxicity Induced by Chemotherapeutic Drugs". 593-612. 2018.
Conference abstract
  1. Marta Leal Bento; Luís carvalho; Zhewei Chen; Coelho, Ana R; Tang, Cong; Gonçalo J. L. Bernardes. "Acute Myeloid and Lymphoblastic Leukemias: A NPM1 Targeting Strategy". Paper presented in ASH, 2023.
  2. "Antiviral NL-CVX1 Efficiently Blocks Infection of SARS-CoV-2 Viral Variants of Concern (VOC)". Paper presented in IDWeek 2021 - Open Forum Infectious Diseases, 2021.
    Published
Conference paper
  1. Coelho, Ana R; J. Kovarova; M. Bajzikova; J. Rohlena; Oliveira, Paulo J.; Jiri Neuzil. "Mitochondrial dihydroorotate dehydrogenase plays an important role in tumor formation". Paper presented in 53rd Annual Scientific Meeting of the European Society for Clinical Investigation, Coimbra, 2019.
  2. "Mitochondrial dihydroorotate dehydrogenase plays an important role in tumor formation". 2019.
    10.1111/eci.13108
  3. J. Rohlena; S. Boukalova; Coelho, Ana R; J. Kovarova; M. Bajzikova; S. Oh; K. Rohlenova; et al. "Reactivation of dihydroorotate dehydrogenase by respiration restores tumor growth of mitochondrial DNA-depleted cancer cells". Paper presented in 52nd Annual Scientific Meeting of the European Society for Clinical Investigation, Barcelona, 2018.
  4. Cunha-Oliveira, T; Coelho, AR; Cláudia M. Deus; Paulo Jorge Gouveia Simões da Silva Oliveira. "Mitochondrial dysfunction and p53 signaling in mouse stem cell-derived cardiomyocytes exposed to doxorubicin". Paper presented in Annual Meeting of the European Society for Clinical Investigation, Génova, 2017.
  5. Neuzil, Jiri; M. Bajzikova; J. Kovarova; Coelho, Ana R; A. Bezawork-Geleta; J. Rohlena; J. Truksa; et al. "Horizontal mitochondrial transfer and tumour initiation". Paper presented in 50th Annual Scientific Meeting of the European Society for Clinical Investigation, Paris, 2016.
  6. J. Kovarova; M. Bajzikova; Coelho, Ana R; Paulo Jorge Gouveia Simões da Silva Oliveira; M. Berridge; L. Dong; Jiri Neuzil. "Tumour initiation is linked to formation of mitochondrial nucleoids". Paper presented in 50th Annual Scientific Meeting of the European Society for Clinical Investigation, Paris, 2016.
Conference poster
  1. Hiroshi Kumagai; Coelho, Ana R; Junxiang Wan; Hemal Mehta; Kelvin Yen; Hirofumi Zempo; Noriyuki Fuku; et al. "MOTS-c inhibits high-fat diet-induced muscle wasting by suppressing myostatin expression via the PTEN/AKT/FOXO1 signaling pathway". Paper presented in Experimental Biology 2020, 2020.
  2. Coelho, Ana R; Su-Jeong Kim; Wan, Junxiang; Oliveira, PJ; Pinchas Cohen. "Myostatin modulation by MOTS-c may lead to beneficial effects in muscle and fat tissue". Paper presented in GSA 2018 Annual Scientific Meeting, 2018.
  3. Coelho, Ana R; Kovarova, Jaromira; Bajzikova, Martina; Rohlena, Jakub; Oliveira, PJ; Berridge, MV; Dong, Lanfeng; Neuzil, Jiri. "The Role of Mitochondrial Transfer from Stroma to mtDNA-devoid Cancer Cells in Tumor Initiation". Paper presented in FEBS - Oncometabolism: from conceptual knowledge to clinical applications., 2017.
  4. Coelho, Ana R; Kovarova, Jaromira; Bajzikova, Martina; Svec, David; Kluckova, Katarina; Chatre, Laurent; Ricchetti, Miria; et al. "Time-resolved analysis of mitochondrial transfer to mtDNA-devoid cancer cells in vivo defines importance of respiration restoration for tumor formation". Paper presented in EMBO Workshop - Mitochondria, apoptosis and cancer, 2017.
  5. Coelho, Ana R; Serafim, Teresa L.; Oliveira PJ. "Role of Sirtuin 3 on Doxorubicin-induced Toxicity on H9c2 Cardiomyoblasts.". Paper presented in 48th Annual Scientific Meeting of the European Society for Clinical Investigation, 2014.
Journal article
  1. Maria Rebelo; Cong Tang; Ana R Coelho; Carlos Labão-Almeida; Matthias M Schneider; Laurie Tatalick; Pedro Ruivo; et al. "De Novo Human Angiotensin-Converting Enzyme 2 Decoy NL-CVX1 Protects Mice From Severe Disease After Severe Acute Respiratory Syndrome Coronavirus 2 Infection". The Journal of Infectious Diseases (2023): http://dx.doi.org/10.1093/infdis/jiad135.
    10.1093/infdis/jiad135
  2. Sigitas Mikutis; Maria Rebelo; Eliza Yankova; Muxin Gu; Cong Tang; Ana R. Coelho; Mo Yang; et al. "Proximity-Induced Nucleic Acid Degrader (PINAD) Approach to Targeted RNA Degradation Using Small Molecules". ACS Central Science 9 5 (2023): 892-904. http://dx.doi.org/10.1021/acscentsci.3c00015.
    10.1021/acscentsci.3c00015
  3. Cong Tang; Ana R. Coelho; Maria Rebelo; Hannah Kiely-Collins; Tânia Carvalho; Gonçalo J. L. Bernardes. "A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen Species". ACS Central Science 9 1 (2023): 109-121. http://dx.doi.org/10.1021/acscentsci.2c01243.
    10.1021/acscentsci.2c01243
  4. Bárbara B. Sousa; Cátia Rebelo de Almeida; Ana F. Barahona; Raquel Lopes; Ana Martins-Logrado; Marco Cavaco; Vera Neves; et al. "Selective Inhibition of Bruton’s Tyrosine Kinase by a Designed Covalent Ligand Leads to Potent Therapeutic Efficacy in Blood Cancers Relative to Clinically Used Inhibitors". ACS Pharmacology Translational Science 5 11 (2022): 1156-1168. http://dx.doi.org/10.1021/acsptsci.2c00163.
    10.1021/acsptsci.2c00163
  5. Kumagai, Hiroshi; Coelho, Ana Raquel; Wan, Junxiang; Mehta, Hemal; Yen, Kelvin; Huang, Amy; Zempo, Hirofumi; et al. "MOTS-c reduces myostatin and muscle atrophy signaling". American Journal of Physiology-Endocrinology and Metabolism (2021): http://dx.doi.org/10.1152/ajpendo.00275.2020.
    10.1152/ajpendo.00275.2020
  6. Oliveira, G.L.; Coelho, A.R.; Marques, R.; Oliveira, P.J.. "Cancer cell metabolism: Rewiring the mitochondrial hub". Biochimica et Biophysica Acta - Molecular Basis of Disease 1867 2 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85097519837&partnerID=MN8TOARS.
    10.1016/j.bbadis.2020.166016
  7. Coelho, Ana R.; Oliveira, Paulo J.. "Dihydroorotate Dehydrogenase Inhibitors in SARS-CoV-2 Infection". European Journal of Clinical Investigation (2020): http://dx.doi.org/10.1111/eci.13366.
    10.1111/eci.13366
  8. Hubackova, Sona; Davidova, Eliska; Boukalova, Stepana; Kovarova, Jaromira; Bajzikova, Martina; Coelho, Ana; Terp, Mikkel G.; et al. "Replication and ribosomal stress induced by targeting pyrimidine synthesis and cellular checkpoints suppress p53-deficient tumors". Cell Death & Disease 11 2 (2020): http://dx.doi.org/10.1038/s41419-020-2224-7.
    10.1038/s41419-020-2224-7
  9. Bajzikova, Martina; Kovarova, Jaromira; Coelho, Ana R.; Boukalova, Stepana; Oh, Sehyun; Rohlenova, Katerina; Svec, David; et al. "Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells". Cell Metabolism 29 2 (2019): 399-416.e10. http://dx.doi.org/10.1016/j.cmet.2018.10.014.
    10.1016/j.cmet.2018.10.014
  10. Cunha-Oliveira, Teresa; Ferreira, Luciana L.; Coelho, Ana Raquel; Deus, Cláudia M.; Oliveira, Paulo J.. "Doxorubicin triggers bioenergetic failure and p53 activation in mouse stem cell-derived cardiomyocytes". Toxicology and Applied Pharmacology 348 (2018): 1-13. http://dx.doi.org/10.1016/j.taap.2018.04.009.
    10.1016/j.taap.2018.04.009
  11. Ana R. Coelho; Tatiana R. Martins; Renata Couto; Cláudia Deus; Cláudia V. Pereira; Rui F. Simões; Albert A. Rizvanov; et al. "Berberine-induced Cardioprotection and Sirt3 Modulation in Doxorubicin-treated H9c2 Cardiomyoblasts". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (2017): https://doi.org/10.1016%2Fj.bbadis.2017.07.030.
    10.1016/j.bbadis.2017.07.030
  12. Dong LF; Kovarova J; Bajzikova M; Bezawork-Geleta A; Svec D; Endaya B; Sachaphibulkij K; et al. "Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells.". eLife (2017): http://europepmc.org/abstract/med/28195532.
    Published • 10.7554/elife.22187
  13. Deus CM; Coelho AR; Serafim TL; Oliveira PJ. "Targeting mitochondrial function for the treatment of breast cancer.". (2014): http://europepmc.org/abstract/med/25365234.
    10.4155/fmc.14.100
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/10/13 G4-RNA Degraders - A novel approach to target SARS-CoV-2 infection ACORN summer school - Chemical Biology of Carbon Monoxide Symposium
(Portugal)
2019/12/20 Horizontal Mitochondrial Transfer to mtDNA-devoid Cancer Cells and the Role of Dihydroorotate Dehydrogenase in Tumor Initiation CIBB meeting 2019
(Coimbra, Portugal)
2019/05/23 Dihydroorotate Dehydrogenase plays an important role in tumor formation 53rd Annual Scientific Meeting of the European Society for Clinical Investigation
(Coimbra, Portugal)
2018/10/22 Horizontal Mitochondrial Transfer to mtDNA-devoid Cancer Cells and the Role of Dihydroorotate Dehydrogenase in Tumor Initiation Mitochondrial DNA and Derived Peptides in Health and Disease
(Coimbra, Portugal)

Event organisation

Event name
Type of event (Role)
Institution / Organization
2023/05/01 - Current iMM Postdoc Retreat (2023/10/19 - 2023/10/20)
Meeting (Member of the Organising Committee)
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
2019/05 - 2019/05 53rd Annual Scientific Meeting of the European Society for Clinical Investigation (2019/05/22)
Congress (Other)
2018/10 - 2018/10 Mitochondrial DNA and Derived Peptides in Health and Disease Meeting (2018/10/22)
Meeting (Member of the Organising Committee)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Fundação Luso-Americana, Portugal
2016/09 - 2016/09 3rd BEB Symposium (2016/09)
Symposium (Member of the Organising Committee)
2012/02 - 2012/02 VII Jornadas de Cardiopneumologia (2012/02)
Conference (Member of the Organising Committee)
Instituto Politécnico de Coimbra Escola Superior de Tecnologia da Saúde de Coimbra, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2022/04 - 2022/04 Leadership and Management Skills Course
Workshop
HPF Consulting, Germany
2019/12 - 2019/12 Training Course in Aromatic Plants and Essential Oils
Workshop
Universidade de Coimbra Faculdade de Farmácia, Portugal
2017/03 - 2017/03 Laboratory Animal Science Course -
Workshop
FELASA Category B Course
Humboldt-Universität zu Berlin, Germany
2015 - 2015 Single-Cell Gene Expression Profiling Course
Workshop
TATTA Biocenter, Czech Republic
2012 - 2012 5th Mayo Munster Coimbra Cardiovascular Meeting
Meeting
Centro Hospitalar e Universitario de Coimbra EPE Campus dos Hospitais da Universidade de Coimbra, Portugal
2009 - 2012 Semana das ciências aplicadas na saúde - Health promotion and disease prevention and screenings
Other
Instituto Politécnico de Coimbra Escola Superior de Tecnologia da Saúde de Coimbra, Portugal
2009 - 2009 Basic Life Support Workshop
Workshop
Seminário de Cardiologia
Instituto Politécnico de Coimbra Escola Superior de Tecnologia da Saúde de Coimbra, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2021/02 - 2021/02 Molecular Biology of Cancer Universidade de Coimbra, Portugal
2019/03/11 - 2019/03/15 Advanced Courses - Oncometabolism: Principles and Applications (Curso de doutoramento (conclusão de unidades curriculares)) Universidade de Coimbra, Portugal

Mentoring / Tutoring

Topic Student name
2021/09 - Current New Therapeutic Options for AML and ALL - BCL-2 RNA degraders Marta Bento
2021 - Current Exercise as an anti-aging strategy to prevent female reproductive decline Maria Moreira Soares
2021/01 - 2021/02 Skin-aging and Mitochondrial-targeted antioxidants Catarina Mendes
2020/07 - 2020/08 Aging-associated diseases and mitochondria Joana Cunha
2019/01 - 2019/02 Mitochondrial Transfer and Mitophagy Francisco de Carvalho
Distinctions

Award

2015 PhD Individual Fellowship
Fundação para a Ciência e a Tecnologia, Portugal
2008 Finalist Best Student of Clinical Physiology
Instituto Politécnico de Coimbra Escola Superior de Tecnologia da Saúde de Coimbra, Portugal

Other distinction

2017 Travel Award for MAC Conference 2017
Gesellschaft zur Förderung der Lebenswissenschaften Heidelberg GmbH, Germany