???global.info.a_carregar???
Isaura Simões graduated in Biochemistry (BSc.1996) at the University of Coimbra, Portugal. She got her MSc in Cell Biology at the University of Coimbra in 1998, and her Ph.D. in 2004 in Biochemistry - Enzymology at the University of Coimbra. She pursued post-doctorate studies at the Center for Neuroscience and Cell Biology of Coimbra (CNC) developing work on structure-function relationships of plant atypical aspartic proteases. In 2006 she joined Biocant - Biotechnology Innovation Center and was involved in the implementation of the Molecular Biotechnology Unit. In 2008 and 2009, Isaura Simões was an Invited Professor at the Department of Biochemistry of the University of Coimbra, responsible for two core disciplines (Molecular Biology and Laboratories of Molecular Biology). In 2010, she joined CNC as an Auxiliary Researcher. All her research activities have been developed at the Molecular Biotechnology Unit in Biocant, and are currently based (since October 2013) at the new UC-Biotech Building in Cantanhede. She was also a Visiting Researcher at the University of Massachusetts (2007), and she was co-founder and manager of Cynara Lda., a spin-off company from Biocant (2011-2014). Recently, she had an appointment as a Visiting Assistant Professor at the Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, USA (Oct2015-Dec2016). Isaura Simões is currently the leader of the Microbial and Molecular Biotechnology Group at CNC. Her research activities as a PI and co-PI have been extramurally funded by governmental agencies and venture capital funds. She established over 15 scientific collaborations with national and international researchers, as well as companies. Scholarly activity associated with her research and mentoring has resulted in 27 peer-reviewed publications (17 as senior corresponding author), 4 granted patents, co-founding of a spin-off company, and numerous presentations. Isaura Simões trained 1 Post-doc, 3 Ph.D. students, 11 MSc students, and 10 research fellows. She has participated in the organization of seminars and international courses as well as in numerous outreach activities. She served in the Scientific Committees of the 30th & 31st Meetings of the American Society for Rickettsiology (2019/2022), as Co-Editor of Research Topic “Obligate Intracellular Bacteria:Evasion & Adaptative Tactics Shaping the Host-Pathogen Interface”, in Front Cell Infect Microbiol (2021), as Review Editor (Molecular Bacterial Pathogenesis section) in the same Journal (2021-), and in peer-review process in many journals. Her group has made significant contributions both in fundamental aspects of structure-function relationships of aspartic proteases as well as in exploring the biotechnological potential of these enzymes. Isaura Simões' group pioneer work on a novel retropepsin-type protease (APRc) from Rickettsia, revealed a new and highly conserved putative virulence factor, whose function and potential targetability are currently being explored in her lab. Her group's studies on the interaction between Spotted fever group (SFG) Rickettsia and macrophages revealed macrophage colonization as a new virulence trait, opening several exciting hypotheses that they are currently validating. Therefore, Isaura Simões' current research program aims at exploring two understudied aspects of rickettsial biology: 1) How proteolytic pathways modulate Rickettsia-host cell interactions; and 2) How pathogenic Rickettsia hijack macrophage function. This will illuminate new factors or molecular pathways critical to developing new therapeutic interventions.
Identification

Personal identification

Full name
Isaura Simões

Citation names

  • Simões, Isaura

Author identifiers

Ciência ID
221E-6FCC-8C0A
ORCID iD
0000-0002-9331-6340

Knowledge fields

  • Medical and Health Sciences - Health Sciences - Infectious Diseases
  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Natural sciences - Biological Sciences - Biochemistry
  • Natural sciences - Biological Sciences - Cell Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2004
Concluded
Ph.D. Biochemistry (Doutoramento)
Major in Especialidade: Enzimologia
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Pldalpha Is a Cardosin a Binding Protein. Identification and Characterization of the Interaction Reveals Kge as a Novel Protein Recognition Sequence" (THESIS/DISSERTATION)
Approved with Distinction and Honours
1998
Concluded
M.Sc. Cell Biology, University of Coimbra, Portugal (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Molecular Characterization of the action of Cardosins A and B on Bovine k- and b-caseins" (THESIS/DISSERTATION)
Very Good
1996
Concluded
B.Sc. in Biochemistry (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
15 (scale 0-20)
Affiliation

Science

Category
Host institution
Employer
2010/10/01 - Current Auxiliary Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2006/01/01 - 2010/09/30 Auxiliary Researcher (Research) Biocant Associação de Transferência de Tecnologia, Portugal
2007/09/01 - 2007/12/22 Visiting Researcher (Research) University of Massachusetts Amherst, United States
2004/09/01 - 2005/12/31 Postdoc (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
1997/01/01 - 1998/12/31 Research Assistant (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Teaching in Higher Education

Category
Host institution
Employer
2008 - 2009 Invited Assistant Professor (University Teacher) Universidade de Coimbra, Portugal
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal

Positions / Appointments

Category
Host institution
Employer
2017 - Current Group Leader, Microbial and Molecular Biotechnology Group Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2015/10/27 - 2016/12/22 Visiting Assistant Professor, Department of Pathobiological Sciences, Vector-borne Disease Laboratories Louisiana State University School of Veterinary Medicine, United States

Others

Category
Host institution
Employer
1998/01/01 - 2002/09/30 PhD student (BD fellowship PRAXIS XXI/FCT) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2001/04/01 - 2001/07/31 Visiting student (PhD project), Professor Alice Cheung Lab University of Massachusetts Amherst, United States
Projects

Grant

Designation Funders
2020 - Current Deciphering the Rickettsia toolbox to hijack the host
ESCMID Research Grant 2020
Other
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
European Society of Clinical Microbiology and Infectious Diseases
Ongoing
2018 - Current Hijack the host or get killed: delineating Rickettsia survival strategies for development of targeted therapeutic strategies
POCI-01-0145-FEDER-029592
PTDC/SAU-INF/29592/2017
Principal investigator
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2020 - 2022 A SAÚDE NO SABER /Health in Knowledge
A SAÚDE NO SABER
Team Member
Concluded
2014 - 2017/04 The retropepsin-like enzyme RC1339/APRick from Rickettsiae as a potential therapeutic target for rickettsial diseases
1R21-AI111086
Research Fellow
Louisiana State University School of Veterinary Medicine, United States
National Institutes of Health Office of Extramural Research
Concluded
2015 - 2017 Plant Aspartic Protease Substrate Identification
Project 6818
Principal investigator
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia

Deutscher Akademischer Austauschdienst
Concluded
2015 - 2015 Structural characterization of the soluble domain of APRc, a novel membrane embedded retropepsin-like aspartic protease from the pathogenic bacteria Rickettsia conorii.
PID: 1310
Principal investigator
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Instruct-ERIC
Concluded
2015 - 2015 Structural characterization of AtLURE1.2: an attraction molecule responsible for guiding pollen tubes to the ovular micropyle in Arabidopsis thaliana.
10029
Principal investigator
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Instruct-ERIC
Concluded
2010 - 2013/07/31 Retroviral-like membrane-associated aspartic proteinases from Rickettsiae: potential new therapeutic targets for rickettsioses.
Principal investigator
Biocant Associação de Transferência de Tecnologia, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2010 - 2013/06 RECOMBINANT PLANT-DERIVED RENNETS FOR CHEESE INDUSTRY
Supervisor
Biocant Associação de Transferência de Tecnologia, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2003 - 2006 Aspartic proteinase content of Arabidopsis genome: identification, expression and characterization
POCTI/BCI/48400/2002
Researcher
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia

Biocant Ventures
Concluded

Contract

Designation Funders
2007 - 2009 Generating a rennet substitute of plant origin based on the natural diversity of cardosin-like proteases.
Biocant/Rennet/2007
Principal investigator
Biocant Associação de Transferência de Tecnologia, Portugal
Biocant Ventures
Concluded
Outputs

Publications

Book chapter
  1. Castanheira, Pedro; Almeida, Carla; Dias-Pedroso, Daniela; Simões, Isaura. "Expression in Escherichia coli, Refolding, and Purification of Plant Aspartic Proteases". In Plant Proteases and Plant Cell Death, 21-33. Springer US, 2022.
    Published • 10.1007/978-1-0716-2079-3_3
Conference abstract
  1. Simões, Isaura; Curto, Pedro; Santa, Cátia; Manadas, Bruno; Martinez, Juan J.. "A pathogen and a non-pathogen SFG Rickettsia trigger differential metabolic signatures in macrophage-like cells". Paper presented in EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Coimbra, 2019.
  2. Li, Mi; Gustchina, Alla; Simões, Marisa; Cruz, Rui Gonçalo Batista Mamede da; Curto, Pedro; Martinez, Juan J.; Simões, Isaura. "Crystal structure of the soluble domain of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease". Paper presented in Foundations of Crystallography, 2017.
  3. Gustchina, Alla; Li, Mi; Cruz, Rui Gonçalo Batista Mamede da; Simões, Marisa; Curto, Pedro; Martinez, Juan J.; Faro, Carlos; Simões, Isaura; Wlodawer, Alexander. "MS7-P4 Crystal structure of the soluble domain of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease". Paper presented in Foundations of Crystallography, 2016.
  4. Almeida, Carla Malaquias; Antunes, Liliana; Simões, Isaura; Faro, Carlos. "Kluyveromyces lactis as a candidate model system for analysis of secretory pathways of plant proteins". Paper presented in YEAST, 2013.
  5. Curto, Pedro; Lufrano, Daniela; Pinto, Cátia; Custódio, Valéria; Gomes, Ana Catarina; Vairo-Cavalli, Sandra; Faro, Carlos; Simões, Isaura. "Kluyveromyces lactis as an attractive expression platform for production of PSI-like antimicrobial peptides". Paper presented in Yeast, 2013.
  6. Almeida, Carla Malaquias; Faro, Carlos; Simões, Isaura. "Development of a new synthetic plant-derived rennet and its method of production in K. lactis". Paper presented in Yeast, 2013.
  7. Curto, Pedro; Pinto, Cátia; Custódio, Valéria; Lufrano, Daniela; Vairo-Cavalli, Sandra; Gomes, Ana Catarina; Faro, Carlos; Simões, Isaura. "Antifungal activity of the plant specific insert domain against phytopathogenic fungi: P14-40". Paper presented in The Febs Journal, 2012.
  8. Lufrano, Daniela; Curto, Pedro; Simões, Isaura; Trejo, Sebastián A.; Lorenzo, J; Vairo-Cavalli, Sandra; Bakás, Laura; Faro, Carlos. "Analysis of the expression of plant specific insert (PSI) domain in the non conventional system Kluveromyces lactis: P03-59". Paper presented in The Febs Journal, 2012.
  9. Leal, Ana Rita; Faro, Rosário; Faro, Carlos; Simões, Isaura. "Evidence forin vivoexpression of shewasins A and D, two pepsin homologs from bacteria: P20-224". Paper presented in The Febs Journal, 2012.
Conference poster
  1. Curto, Pedro; Santa, Cátia; Manadas, Bruno; Martinez, Juan J.; Simões, Isaura. "A pathogen and a non-pathogen SFG Rickettsia trigger differential metabolic signatures in macrophage-like cells.". Paper presented in 53rd Annual Meeting of the European Society for Clinical Investigation (ESCI), Coimbra, Portugal, 2019.
  2. Curto, Pedro; Ferreira, Andreia; Lopes, Marisa; Cruz, Rui Gonçalo Batista Mamede da; Antunes, Liliana; Simões, Isaura. "Understanding the molecular determinants of autoprocessing of APRc, the retropepsin-like homolog from Rickettsia.". Paper presented in 11th General Meeting of the International Proteolysis Society, 29 Sep - 4 Oct 2019, Mariánské Lázne, Czech Republic, 2019.
  3. Almeida, Carla Malaquias; Grãos, Mário; Faro, Carlos; Simões, Isaura. "VSkin - A synthetic plant-derived skin desquamation agent for cosmetics and therapeutics applications.". Paper presented in MICROBIOTEC19 Congress of Microbiology and Biotechnology 19, 5-7 December, Coimbra, Portugal, 2019.
  4. Curto, Pedro; Santa, Cátia; Manadas, Bruno; Simões, Isaura; Martinez, Juan J.. "Host players involved in the Interaction between Spotted Fever Group Rickettsia and Macrophage-like cells.". Paper presented in EMBO Conference on Hijacking host signalling and epigenetic mimicry during infections, 13-16 June, Paris, France., 2017.
  5. Simões, Isaura; Curto, Pedro; Santa, Cátia; Manadas, Bruno; Martinez, Juan J.. "Interaction between Spotted Fever Group Rickettsia and macrophage-like cells: Glimpse into global host responses triggered by a pathogenic and a non-pathogenic species.". Paper presented in 10th General Meeting of the International Proteolysis Society, 28 October – 1 November, Banff, Canada., 2017.
  6. Cruz, Rui Gonçalo Batista Mamede da; Li, Mi; Gustchina, Alla; Curto, Pedro; Simões, Marisa; Huesgen, Pitter F.; Overall, Christopher M.; et al. "Retropepsin-like enzymes in bacteria: lessons from rickettsiae.". Paper presented in MICROBIOTEC17 Congress of Microbiology and Biotechnology 17, 7-9 December, Porto, Portugal, 2017.
  7. Almeida, Carla Malaquias; Manso-Garcia, J.A.; Figueiredo, A.C.; Antunes, Liliana; Cruz, Rui Gonçalo Batista Mamede da; Manadas, Bruno; Bur, Daniel; et al. "A synthetic cardosin b-derived rennet for sheep and goat cheese manufacture.". Paper presented in MICROBIOTEC17 Congress of Microbiology and Biotechnology 17, 7-9 December, Porto, Portugal, 2017.
  8. Curto, Pedro; Santa, Cátia; Manadas, Bruno; Simões, Isaura; Martinez, Juan J.. "New insights on the routes of entry of two spotted fever group rickettsia in macrophage-like cells.". Paper presented in MICROBIOTEC17 Congress of Microbiology and Biotechnology 17, 7-9 December, Porto, Portugal, 2017.
  9. Lourenço, A.S.T.; Mas, P.J.; Anjo, Sandra Isabel; Euclides, Pires; Manadas, Bruno; Macedo-Ribeiro, Sandra; Simões, Isaura; et al. "Structural and interactomic characterization of SAPAP3 - a postsynaptic density scaffolding protein involved in Obsessive Compulsive Disorder.". Paper presented in XXVI Paulo Foundation Symposium “From Atomic Structures to Disease Mechanisms", 2015.
  10. Soares, André; Faro, Rosário; Loos, Andreas; Steinkellner, Herta; Huesgen, Pitter F.; Faro, Carlos; Cheung, Alice Y.; Simões, Isaura. "RLR1 and RLR2, two novel Arabidopsis thaliana atypical aspartic proteases involved in primary root development and lateral root formation". Paper presented in 11th International Congress of Plant Molecular Biology, Brazil., 2015.
  11. Soares, André; Niedermaier, Stefan; Faro, Rosário; Loos, Andreas; Steinkellner, Herta; Huesgen, Pitter F.; Faro, Carlos; Cheung, Alice Y.; Simões, Isaura. "RLR1 and RLR2, two novel Arabidopsis thaliana atypical aspartic proteases involved in primary root development and lateral root formation". Paper presented in 9th General Meeting of the International Proteolysis Society,4-8 October, Penang, Malaysia., 2015.
  12. Cruz, Rui Gonçalo Batista Mamede da; Simões, Marisa; Huesgen, Pitter F.; Overall, Christopher M.; Faro, Carlos; Martinez, Juan J.; Simões, Isaura. "Biochemical and enzymatic studies on APRick, the retropepsin-like homologue from Rickettsia conorii.". Paper presented in 26th Meeting of the American Society for Rickettsiology, June 15-18, 2013, Portland, Maine, USA., 2013.
  13. Almeida, Carla Malaquias; Simões, Isaura; Faro, Carlos. "Development of a new synthetic plant-derived rennet and its method of production in Kluyveromyces lactis.". Paper presented in 26th International Conference on Yeast Genetics and Molecular Biology, Satellite Symposium 1, Kluyveromyces Satellite Symposium, August 29th to September 3rd, Frankfurt, Germany. Yeast, Volume: 30 Pages: 136., 2013.
  14. Almeida, Carla Malaquias; Simões, Isaura; Faro, Carlos. "Development of a new plant-derived rennet adapted for sheep and goat cheese production.". Paper presented in 8th NIZO Dairy Conference, 11-13 September 2013, Papendal, The Netherlands., 2013.
  15. Almeida, Carla Malaquias; Simões, Isaura; Faro, Carlos. "The path to a new synthetic plant-derived rennet.". Paper presented in 8th General Meeting of the International Proteolysis Society, 20-24 October, Spier Estate, Cape Town, South Africa., 2013.
  16. Soares, André; Faro, Rosário; Loos, Andreas; Steinkellner, Herta; Cheung, Alice Y.; Simões, Isaura. "Production, purification and biochemical characterization of Q84M99, a novel Arabidopsis thaliana Atypical Aspartic Protease involved in pollen development.". Paper presented in CNC Annual Meeting 18-19 December, Cantanhede, Portugal., 2013.
  17. Lufrano, Daniela; Curto, Pedro; Simões, Isaura; Trejo, Sebastián A.; Lorenzo, J; Vairo-Cavalli, Sandra; Bakás, Laura; Faro, Carlos. "Análisis de la expresión del dominio inserto específico de planta en el sistema no convencional Kluveromyces lactis.". Paper presented in 35º Congreso de la SEBBM, 4-9 September, Seville, Spain. FEBS Journal Volume: 279 Pages: 84-85, 2012.
  18. Curto, Pedro; Pinto, Cátia; Custódio, Valéria; Lufrano, Daniela; Vairo-Cavalli, Sandra; Gomes, Ana Catarina; Faro, Carlos; Simões, Isaura. "Antifungal activity of the plant specific insert domain against phytopathogenic fungi.". Paper presented in 22nd IUBMB & 37th FEBS Congress, 4-9 September, Seville, Spain. FEBS Journal Volume: 279 Pages: 341., 2012.
  19. Leal, Ana Rita; Faro, Rosário; Faro, Carlos; Simões, Isaura. "Evidence for in vivo expression of shewasins A and D, two pepsin homologs from bacteria.". Paper presented in 22nd IUBMB & 37th FEBS Congress, 4-9 September, Seville, Spain. FEBS Journal Volume: 279 Pages: 475-476., 2012.
  20. Simões, Isaura; Cruz, Rui Gonçalo Batista Mamede da; Antunes, Liliana; Faro, Rosário; Faro, Carlos. "APRick, an active retroviral-like aspartic proteinase homologue from Rickettsia conorii.". Paper presented in 6th International Meeting on Rickettsiae and Rickettsial Diseases. 5-7 June 2011, Heraklion, Crete, Greece., 2011.
  21. Cruz, Rui Gonçalo Batista Mamede da; Antunes, Liliana; Faro, Rosário; Faro, Carlos; Simões, Isaura. "An active retroviral-like aspartic proteinase homologue from Rickettsia conorii.". Paper presented in 7th General Meeting of the International Proteolysis Society. 16-20 October, San Diego, California, USA. (Rui Cruz was awarded with a Travel Award from the IPS)., 2011.
  22. Simões, Isaura; Leal, Ana Rita; Faro, Rosário; Bur, Daniel; Kay, John. "Shewasins, active pepsin homologues from the bacteria Shewanella amazonensis and Shewanella denitrificans.". Paper presented in 7th General Meeting of the International Proteolysis Society. 16-20 October, San Diego, California, USA., 2011.
  23. Cruz, Rui Gonçalo Batista Mamede da; Faro, Rosário; Faro, Carlos; Simões, Isaura. "Characterization of recombinant APRick, a novel retroviral-like aspartic proteinase from Rickettsia conori.". Paper presented in XVII Congresso Nacional de Bioquímica. 15-17 December, Porto, Portugal., 2010.
  24. Simões, Isaura; Faro, Rosário; Bur, Daniel; Faro, Carlos. "Characterization of recombinant CDR1, an Arabidopsis aspartic proteinase involved in disease resistance.". Paper presented in 5th General Meeting of the International Proteolysis Society (IPS2007), 20-25 de Outubro, Patras, Greece., 2007.
  25. Simões, Isaura; Faro, Rosário; Bur, Daniel; Faro, Carlos. "Cloning, expression and characterization of CDR1, an atypical Arabidopsis aspartic proteinase involved in defense against pathogens.". Paper presented in 15º Congresso Nacional de Bioquímica, 8-10 de Dezembro, Aveiro, Portugal., 2006.
  26. Simões, Isaura; Faro, Rosário; Bur, Daniel; Faro, Carlos. "Cloning, expression and characterization of recombinant CDR1, an atypical aspartic proteinase from Arabidopisis thaliana.". Paper presented in 4th General Meeting of the International Proteolysis Society – Associated with the International Conference on Protein Inhibitors, 15-19 October, Quebec City, Canada., 2005.
  27. Simões, Isaura; Mueller, Eva-Christina; Faro, Carlos; Pires, Euclides. "Cardosin A associates directly with the C2 domain of phopholipase Da via a KGE sequence motif.". Paper presented in International Conference on Aspartic Proteases and Inhibitors 2003, ICAPI 03, 14-16 November, Kyoto, Japan., 2003.
  28. Simões, Isaura; Mueller, Eva-Christina; Faro, Carlos; Pires, Euclides. "Identification of PLD as a Cardosin A interacting protein.". Paper presented in FEBS Forum for Young Scientists, 18-20 October, Istanbul, Turkey., 2002.
  29. Simões, Isaura; Mueller, Eva-Christina; Faro, Carlos; Pires, Euclides. "Identification of PLD as a Cardosin A interacting protein.". Paper presented in 28th meeting of the Federation of European Biochemical Societies, 20-25 October, Istanbul, Turkey., 2002.
  30. Simões, Isaura; Mueller, Eva-Christina; Faro, Carlos. "PLD as a Cardosin A interacting protein.". Paper presented in XIII Meeting of Portuguese Biochemical Society, 5-7 December, Lisboa, Portugal., 2002.
  31. Simões, Isaura; Mueller, Eva-Christina; Faro, Carlos; Pires, Euclides. "Identification of an RGD binding protein present in the pollen of Cynara cardunculus L.". Paper presented in EMBO Lecture Course in Cellular Signaling in Development and Disease, 21-27 de September 2001, Split, Croatia, 2001.
  32. Faro, Carlos; Veríssimo, Paula; Ramalho-Santos, Miguel; Pissarra, José; Vieira, Margarida; Simões, Isaura; Pires, Euclides. "A role for cardosins in pollen-pistil interaction.". Paper presented in VIIIth International Aspartic Proteinase Conference, 7-12 September, Funchal, Madeira, Portugal., 1999.
  33. Simões, Isaura; Ramalho-Santos, Miguel; Veríssimo, Paula; Faro, Carlos; Pires, Euclides. "Identification of a putative Cardosin A receptor present in the pollen of Cynara cardunculus L.". Paper presented in VIIIth International Aspartic Proteinase Conference, 7-12 September, Funchal, Madeira, Portugal., 1999.
  34. Simões, Isaura; Veríssimo, Paula; Faro, Carlos; Pires, Euclides. "Hydrolysis of beta-casein by cardosins A and B, the aspartic proteinases from Cynara cardunculus L.". Paper presented in BIOTEC’98, The IV Iberian Congress on Biotechnology and the I Ibero-American Meeting on Biotechnology, 12-15 July. Guimarães, Portugal, 1998.
  35. Simões, Isaura; Veríssimo, Paula; Faro, Carlos; Pires, Euclides. "Action on Bovine kappa-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L.". Paper presented in XI Meeting of Portuguese Biochemical Society, 1998.
  36. Simões, Isaura; Veríssimo, Paula; Faro, Carlos; Pires, Euclides. "Action of cardosins A and B, two aspartic proteinases from cardoon, on bovine beta-casein.". Paper presented in XI Meeting of Portuguese Biochemical Society, 28-30 November. Tomar, Portugal., 1996.
Journal article
  1. Martins-Marques, Tania; Costa, Marina C; Catarino, Steve; Simoes, Isaura; Aasen, Trond; Enguita, Francisco J; Girao, Henrique. "Cx43-mediated sorting of miRNAs into extracellular vesicles". EMBO reports (2022): http://dx.doi.org/10.15252/embr.202154312.
    Published • 10.15252/embr.202154312
  2. Matos, Ana Luísa; Curto, Pedro; Simões, Isaura. "Moonlighting in Rickettsiales: Expanding Virulence Landscape". Tropical Medicine and Infectious Disease 7 2 (2022): 32. http://dx.doi.org/10.3390/tropicalmed7020032.
    10.3390/tropicalmed7020032
  3. Curto, Pedro; Santa, Cátia; Cortes, Luísa; Manadas, Bruno; Simões, Isaura. "Spotted Fever Group Rickettsia Trigger Species-Specific Alterations in Macrophage Proteome Signatures with Different Impacts in Host Innate Inflammatory Responses". Microbiology Spectrum 9 3 (2021): http://dx.doi.org/10.1128/spectrum.00814-21.
    10.1128/spectrum.00814-21
  4. Curto, Pedro; Barro, Andreia; Almeida, Carla; Vieira-Pires, Ricardo S.; Simões, Isaura. "The Retropepsin-Type Protease APRc as a Novel Ig-Binding Protein and Moonlighting Immune Evasion Factor of Rickettsia". mBio 12 6 (2021): http://dx.doi.org/10.1128/mbio.03059-21.
    10.1128/mbio.03059-21
  5. Figueiredo, Pedro R.; Santos, Sónia F. G.; Almeida, Beatriz C.; Simões, Isaura; Carvalho, Alexandra T. P.. "Introduction of a Glycine Linker Connecting the Heavy and Light Chains in Synthetic Cardosin B-Derived Rennet Changes the Specificity of Subpocket S3'". The Journal of Physical Chemistry B 125 17 (2021): 4368-4374. http://dx.doi.org/10.1021/acs.jpcb.1c01826.
    10.1021/acs.jpcb.1c01826
  6. Barracosa, P.; Simões, I.; Martins, A.P.; Barros, M.; Pires, E.. "Biochemical diversity of cardoon flowers (Cynara cardunculus L.): Predicting PDO Mediterranean cheese textures". Food Bioscience 39 (2021): 100805. http://dx.doi.org/10.1016/j.fbio.2020.100805.
    10.1016/j.fbio.2020.100805
  7. Anjo, Sandra Isabel; Simões, Isaura; Castanheira, Pedro; Grãos, Mário; Manadas, Bruno. "Use of recombinant proteins as a simple and robust normalization method for untargeted proteomics screening: exhaustive performance assessment". Talanta 205 (2019): 120163. http://dx.doi.org/10.1016/j.talanta.2019.120163.
    10.1016/j.talanta.2019.120163
  8. Curto, Pedro; Riley, Sean P.; Simões, Isaura; Martinez, Juan J.. "Macrophages Infected by a Pathogen and a Non-pathogen Spotted Fever Group Rickettsia Reveal Differential Reprogramming Signatures Early in Infection". Frontiers in Cellular and Infection Microbiology 9 (2019): http://dx.doi.org/10.3389/fcimb.2019.00097.
    10.3389/fcimb.2019.00097
  9. Curto, Pedro; Santa, Cátia; Allen, Paige; Manadas, Bruno; Simões, Isaura; Martinez, Juan J.. "A Pathogen and a Non-pathogen Spotted Fever Group Rickettsia Trigger Differential Proteome Signatures in Macrophages". Frontiers in Cellular and Infection Microbiology 9 (2019): http://dx.doi.org/10.3389/fcimb.2019.00043.
    10.3389/fcimb.2019.00043
  10. Soares, André; Niedermaier, Stefan; Faro, Rosário; Loos, Andreas; Manadas, Bruno; Faro, Carlos; Huesgen, Pitter F; Cheung, Alice Y; Simões, Isaura. "An atypical aspartic protease modulates lateral root development in Arabidopsis thaliana". Journal of Experimental Botany 70 7 (2019): 2157-2171. http://dx.doi.org/10.1093/jxb/erz059.
    10.1093/jxb/erz059
  11. Soares, André; Ribeiro Carlton, Sofia M; Simões, Isaura. "Atypical and nucellin-like aspartic proteases: emerging players in plant developmental processes and stress responses". Journal of Experimental Botany 70 7 (2019): 2059-2076. http://dx.doi.org/10.1093/jxb/erz034.
    10.1093/jxb/erz034
  12. Almeida, Carla Malaquias; Simões, Isaura. "Cardoon-based rennets for cheese production". Applied Microbiology and Biotechnology 102 11 (2018): 4675-4686. http://dx.doi.org/10.1007/s00253-018-9032-3.
    10.1007/s00253-018-9032-3
  13. Almeida, Carla Malaquias; Manso, José A.; Figueiredo, Ana C.; Antunes, Liliana; Cruz, Rui; Manadas, Bruno; Bur, Daniel; et al. "Functional and structural characterization of synthetic cardosin B-derived rennet". Applied Microbiology and Biotechnology 101 18 (2017): 6951-6968. http://dx.doi.org/10.1007/s00253-017-8445-8.
    10.1007/s00253-017-8445-8
  14. Curto, Pedro; Simões, Isaura; Riley, Sean P.; Martinez, Juan J.. "Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells". Frontiers in Cellular and Infection Microbiology 6 (2016): http://dx.doi.org/10.3389/fcimb.2016.00080.
    10.3389/fcimb.2016.00080
  15. Leal, Ana Rita; Cruz, Rui; Bur, Daniel; Huesgen, Pitter F.; Faro, Rosário; Manadas, Bruno; Wlodawer, Alexander; Faro, Carlos; Simões, Isaura. "Enzymatic properties, evidence for in vivo expression, and intracellular localization of shewasin D, the pepsin homolog from Shewanella denitrificans". Scientific Reports 6 1 (2016): http://dx.doi.org/10.1038/srep23869.
    10.1038/srep23869
  16. Dias, David M.; Furtado, Joana; Wasielewski, Emeric; Cruz, Rui; Costello, Bernard; Cole, Lindsay; Faria, Tiago Q.; et al. "Biophysical characterization of laforin–carbohydrate interaction". Biochemical Journal 473 3 (2016): 335-345. http://dx.doi.org/10.1042/bj20141555.
    10.1042/bj20141555
  17. Li, Mi; Gustchina, Alla; Cruz, Rui; Simões, Marisa; Curto, Pedro; Martinez, Juan; Faro, Carlos; Simões, Isaura; Wlodawer, Alexander. "Structure of RC1339/APRc fromRickettsia conorii, a retropepsin-like aspartic protease". Acta Crystallographica Section D Biological Crystallography 71 10 (2015): 2109-2118. http://dx.doi.org/10.1107/s1399004715013905.
    10.1107/s1399004715013905
  18. Cruz, Rui; Huesgen, Pitter; Riley, Sean P.; Wlodawer, Alexander; Faro, Carlos; Overall, Christopher M.; Martinez, Juan J.; Simões, Isaura. "RC1339/APRc from Rickettsia conorii Is a Novel Aspartic Protease with Properties of Retropepsin-Like Enzymes". PLoS Pathogens 10 8 (2014): e1004324. http://dx.doi.org/10.1371/journal.ppat.1004324.
    10.1371/journal.ppat.1004324
  19. Almeida, Carla Malaquias; Gomes, David; Faro, Carlos; Simões, Isaura. "Engineering a cardosin B-derived rennet for sheep and goat cheese manufacture". Applied Microbiology and Biotechnology 99 1 (2014): 269-281. http://dx.doi.org/10.1007/s00253-014-5902-5.
    10.1007/s00253-014-5902-5
  20. Curto, Pedro; Lufrano, Daniela; Pinto, Cátia; Custódio, Valéria; Gomes, Ana Catarina; Trejo, Sebastián A.; Bakás, Laura; et al. "Establishing the Yeast Kluyveromyces lactis as an Expression Host for Production of the Saposin-Like Domain of the Aspartic Protease Cirsin". Applied and Environmental Microbiology 80 1 (2014): 86-96. http://dx.doi.org/10.1128/aem.03151-13.
    10.1128/aem.03151-13
  21. Resende, Rosa; Marques, Sueli C. F.; Ferreiro, Elisabete; Simões, Isaura; Oliveira, Catarina R.; Pereira, Cláudia M. F.. "Effect of a-Synuclein on Amyloid ß-Induced Toxicity: Relevance to Lewy Body Variant of Alzheimer Disease". Neurochemical Research 38 4 (2013): 797-806. http://dx.doi.org/10.1007/s11064-013-0982-7.
    10.1007/s11064-013-0982-7
  22. Lufrano, Daniela; Faro, Rosário; Castanheira, Pedro; Parisi, Gustavo; Veríssimo, Paula; Vairo-Cavalli, Sandra; Simões, Isaura; Faro, Carlos. "Molecular cloning and characterization of procirsin, an active aspartic protease precursor from Cirsium vulgare (Asteraceae)". Phytochemistry 81 (2012): 7-18. http://dx.doi.org/10.1016/j.phytochem.2012.05.028.
    10.1016/j.phytochem.2012.05.028
  23. Almeida, Carla Malaquias; Pereira, Cláudia; da Costa, Diana Soares; Pereira, Susana; Pissarra, José; Simões, Isaura; Faro, Carlos. "Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii". Planta 236 1 (2012): 283-296. http://dx.doi.org/10.1007/s00425-012-1605-2.
    10.1007/s00425-012-1605-2
  24. Simões, Isaura; Faro, Rosário; Bur, Daniel; Kay, John; Faro, Carlos. "Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis". FEBS Journal 278 17 (2011): 3177-3186. http://dx.doi.org/10.1111/j.1742-4658.2011.08243.x.
    10.1111/j.1742-4658.2011.08243.x
  25. Simões, Isaura; Faro, Rosário; Bur, Daniel; Faro, Carlos. "Characterization of Recombinant CDR1, an Arabidopsis Aspartic Proteinase Involved in Disease Resistance". Journal of Biological Chemistry 282 43 (2007): 31358-31365. http://dx.doi.org/10.1074/jbc.m702477200.
    10.1074/jbc.m702477200
  26. Simões, Isaura; Mueller, Eva-Christina; Otto, Albrecht; Bur, Daniel; Cheung, Alice Y.; Faro, Carlos; Pires, Euclides. "Molecular analysis of the interaction between cardosin A and phospholipase Da". FEBS Journal 272 22 (2005): 5786-5798. http://dx.doi.org/10.1111/j.1742-4658.2005.04967.x.
    10.1111/j.1742-4658.2005.04967.x
  27. Simões, Isaura; Faro, Carlos. "Structure and function of plant aspartic proteinases". European Journal of Biochemistry 271 11 (2004): 2067-2075. http://dx.doi.org/10.1111/j.1432-1033.2004.04136.x.
    10.1111/j.1432-1033.2004.04136.x
  28. Faro, Carlos; Ramalho-Santos, Miguel; Vieira, Margarida; Mendes, Alexandra; Simões, Isaura; Andrade, Rita; Veri´ssimo, Paula; et al. "Cloning and Characterization of cDNA Encoding Cardosin A, an RGD-containing Plant Aspartic Proteinase". Journal of Biological Chemistry 274 40 (1999): 28724-28729. http://dx.doi.org/10.1074/jbc.274.40.28724.
    10.1074/jbc.274.40.28724

Intellectual property

Patent
  1. Almeida, Carla Malaquias; Simões, Isaura; Carlos Faro. 2019. "Use of plant aspartic proteases for treating skin conditions. European Patent No. EP 2 976 356 B1, 2019 Sep 25. Biocant-Associação Transferência de Tecnologia, assignee. GRANTED".
    Granted/Issued
  2. Almeida, Carla Malaquias; Simões, Isaura; Carlos Faro. 2019. "Aspartic Proteases. United States Patent No. 10,196,624, 2019 Feb 5. Biocant-Associação Transferência de Tecnologia, assignee. GRANTED". United States.
    Granted/Issued
  3. Almeida, Carla Malaquias; Simões, Isaura; Carlos Faro. 2018. "Use of plant aspartic proteases for treating skin conditions. European Patent No. EP 2 981 278 B1, 2018 Sep 19. Biocant-Associação Transferência de Tecnologia, assignee. GRANTED".
    Granted/Issued
  4. Almeida, Carla Malaquias; Simões, Isaura; Carlos Faro. 2018. "Aspartic proteases. United States Patent No. 9,890,372, 2018 Feb 13. 1. Biocant-Associação Transferência de Tecnologia, assignee. GRANTED". United States.
    Granted/Issued

Other

Spin-off company
  1. 2011. Cynara Lda. (2011-2014). Co-founder/ Manager, spin-off focused on commercializing synthetic rennet for cheese production.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022 How pathogenic SFG Rickettsia modulate macrophages: new insignts into rickettsial pathogenesis Advanced Course on Bioinformatics for Microbiologists
University of Aveiro
2022 Spotted Fever Group Rickettsia Trigger Alterations in Macrophage Proteome Signatures with Different Impacts in Host Inflammatory Responses Department of Parasitology Institute of Biomedical Sciences, University of São Paulo
2021 Rickettsial subversion strategies to escape host immune defenses. Seminar Series - Spotlight on Rickettsia sponsored by the American Society for Rickettsiology
Invited Speaker, Isaura Simões (United States)
2021 Elucidating how pathogenic spotted fever group Rickettsia modulate proteome signatures in host cells: new insights into rickettsial pathogenesis 7th International Caparica Conference on Analytical Proteomics 2021
(Portugal)
2021 Rickettsial subversion tactics to escape host immune defenses XIII ENEBIOQ 21, XIII Encontro Nacional de Estudantes de Bioquímica
2020 Rickettsial subversion tactics to evade host innate immunity. School of Pharmaceutical Sciences - São Paulo State University. UNESP
Invited Speaker, Isaura Simões (Brazil)
2019 Understanding the molecular determinants of autoprocessing of APRc, the retropepsin-type protease from Rickettsiae 30th Meeting of the American Society for Rickettsiology
(Oral Communication, Isaura Simões) (Santa Fe, United States)
2019 An “Omics” approach to understand rickettsial pathogenesis MICROBIOTEC19 Congress of Microbiology and Biotechnology
(Oral Communication, Curto, P.) (Coimbra, Portugal)
2018 Understanding Rickettsia-host interactions: the protease, the Trojan Horse and other stories. CNC.IBILI Seminars
(Invited Speaker, Isaura Simões) (Coimbra, Portugal)
2018 What can we (still) learn from plant aspartic proteases? 4Th Plant Protease and Programmed Cell Death Symposium.
(Invited Speaker, Isaura Simões) (Ghent, Belgium)
2018 A pathogen and a non-pathogen SFG Rickettsia trigger differential metabolic signatures in macrophage-like cells 29th Meeting of the American Society for Rickettsiology
(Oral Communication, Isaura Simões) (Milwaukee, United States)
2018 Global transcriptomic analysis of macrophages infected by a pathogen and a non-pathogen SFG Rickettsia reveals differential reprogramming of host signaling pathways 28th ECCMID
(Mini-oral ePoster session, Curto, P.) (Madrid, Spain)
2018 Global transcriptomic analysis of macrophages infected by a pathogen and a non-pathogen SFG Rickettsia reveals differential reprogramming of host signaling pathways 29th Meeting of the American Society for Rickettsiology
(Oral communication, Curto, P.) (Milwaukee, United States)
2017 Host players involved in the Interaction between Spotted Fever Group Rickettsia and Macrophage-like cells. ESCCAR 2017 - International Congress on Rickettsia and other Intracellular Bacteria
(Oral Communication, Isaura Simões) (Marseille, France)
2017 Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells. MICROBIOTEC17 Congress of Microbiology and Biotechnology
(Oral Communication, Curto, P.) (Porto, Portugal)
2016 The Retropepsin-like Enzyme RC1339/APRc from Rickettsiae as a Potential Therapeutic Target for Rickettsial Diseases. Enzymes in Drug Discovery Summit – 2nd Protease Inhibitors in Drug Discovery.
(Invited Speaker, Isaura Simões) (San Diego, United States)
2016 What Can (Still) Be Learned From Prokaryotic And Plant Aspartic Proteases? Invited Seminar, Isaura Simões
Macromolecular Crystallography Laboratory, National Cancer Institute (Frederick, United States)
2016 The first crystal structure of the soluble domain of RC1339/APRc from R. conorii reveals a striking conservation with the typical fold of retropepsins. 28th Meeting of the American Society for Ricketsiology
(Oral Communication, Isaura Simões) (Big Sky, Montana, United States)
2016 Differences in the Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells 28th Meeting of the American Society for Ricketsiology
(Oral communication, Curto, P.) (Big Sky, Montana, United States)
2015 Plant Aspartic Proteases: Implications in Developmental Processes and Stress Tolerance. Invited Seminar, Isaura Simões
Central Institute for Engineering, Electronics and Analytics (ZEA-3), Forschungszentrum Jülich (Julich, Germany)
2015 Crystal structure of the soluble domain of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease. 9th General Meeting of the International Proteolysis Society
(Oral Communication, Isaura Simões) (Penang, Malaysia)
2015 RLR1 and RLR2, two novel Arabidopsis thaliana atypical aspartic proteases involved in primary root development and lateral root formation XIII CNC annual meeting
(Oral Communication, Soares,A.) (Coimbra, Portugal)
2014 Development of a new synthetic plant-derived rennet for sheep and goat cheese production. ENZITEC 2014, XI Seminário Brasileiro de Tecnologia Enzimática
(Invited Speaker, Isaura Simões) (Rio de Janeiro, Brazil)
2014 Say Cheese. CNC.IBILI Seminars – Fall Series 2014
(Invited Speaker, Isaura Simões) (Coimbra, Portugal)
2014 Structural and interactomic characterization of SAPAP3 - a postsynaptic density scaffolding protein involved in Obsessive Compulsive Disorder XII CNC Annual Meeting
(Oral Communication, Lourenço, AST) (Coimbra, Portugal)
2013 Optimization of Protein Expression in K. lactis: A Case Study of a Difficult Expression Problem Solved Fifth Annual PEGS EUROPE, Protein & Antibody Engineering Summit
(Invited Speaker, Isaura Simões) (Lisbon, Portugal)
2013 The path to new synthetic plant-derived rennet(s): Optimizing heterologous protein secretion in Kluyveromyces lactis by manipulating protein sorting. 4th P4EU Meeting
(Invited Speaker, Isaura Simões) (Porto, Portugal)
2013 Retropepsin-like enzymes in prokaryotes: lessons from Rickettsiae 8th General Meeting of the International Proteolysis Society
(Oral Communication, Isaura Simões) (Cape Town, South Africa)
2013 Kluyveromyces lactis as an attractive expression platform for production of PSI-like antimicrobial peptides 26th International Conference on Yeast Genetics and Molecular Biology, Satellite Symposium 1, Kluyveromyces Satellite Symposium
(Oral communication, Curto P.) (Frankfurt, Germany)
2013 Kluyveromyces lactis as a candidate model system for analysis of secretory pathways of plant proteins 26th International Conference on Yeast Genetics and Molecular Biology, Satellite Symposium 1, Kluyveromyces Satellite Symposium
(Oral Communication, Almeida, CM)
2013 Biochemical and enzymatic studies on APRick, a novel retropepsin-like enzyme from Rickettsia conorii CNC Annual Meeting
(Oral Communication, Cruz,R.) (Cantanhede, Portugal)
2012 VREN: Desenvolvimento de um coalho inovador de origem vegetal Jornadas Práticas de Laticínios Rabaçal
(Invited Speaker, Isaura Simões) (Rabaçal, Portugal)
2012 Changing the paradigm: May “Prokaryopepsins” be the new archetypes of modern aspartic proteinases? Pep-Con2012 Protein and Peptide Conference
(Invited Speaker, Isaura Simões) (Beijing, China)
2012 APRick, a rickettsial retropepsin-like enzyme, as the candidate enzyme for the processing of rOmpB 25th Meeting of the American Society for Rickettsiology
(Oral Communication, Isaura Simões) (Park City, United States)
2011 Creating Value Through Innovation JORTEC – Jornadas Tecnológicas da FCT/UNL, Universidade Nova de Lisboa
(Invited Speaker, Isaura Simões) (Lisbon, Portugal)
2003 Cardosin A associates directly with the C2 domain of phopholipase Da via a KGE sequence motif. International Conference on Aspartic Proteases and Inhibitors 2003, ICAPI 03
(Oral Communication, Isaura Simões) (Kyoto, Japan)
2003 Cardosin A associates directly with the C2 domain of phopholipase Da via a KGE sequence motif. 3rd General Meeting of the International Proteolysis Society
(Oral Communication, Isaura Simões) (Nagoya, Japan)
1998 Action on Bovine kappa-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L. BIOTEC’98, The IV Iberian Congress on Biotechnology and the I Ibero-American Meeting on Biotechnology
(Oral Communication, Isaura Simões) (Guimarães, Portugal)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2021/02 - Current Production and Purification of Proteases
Co-supervisor
Engenharia Química (Master)
Universidade de Coimbra Departamento de Engenharia Quimica, Portugal
2021 - Current Elucidating the function of the retropepsin APRc as a moonlighting immune evasion factor of Rickettsia
Supervisor
Biologia Celular e Molecular (Master) Universidade de Coimbra, Portugal (Master)
Universidade de Coimbra, Portugal
2019/09 - 2021/02 Characterization of APRc as an immune evasion factor of Rickettsia
Supervisor of Andreia Margarida Silva Barro
Biotecnologia (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/09 - 2020/11 Elucidating autoprocessing activity and function of the rickettsial retropepsin APRc in mammalian cells
Supervisor of Bárbara Daniela Araújo Pinheiro Teixeira
Biologia Celular e Molecular (Master)
Universidade de Coimbra, Portugal
2019/05 - 2019/12 Evaluation of the auto-processing activity of the retropepsin-like enzyme APRc in E. coli extracts
Supervisor of Andreia Filipa Aido Marques Ferreira
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2019/05 - 2019/12 Autoprocessing Activity of APRc in soluble extracts of E. coli
Supervisor of Marisa Mateus Lopes
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2015 - 2018 Rickettsia-macrophage tropism: A link to rickettsial pathogenicity?
Supervisor of Pedro Tiago Cardoso Curto
Universidade de Coimbra, Portugal
2012 - 2017 RLR1 and RLR2, two novel Arabidopsis thaliana atypical aspartic proteases involved in primary root development and lateral root formation
Supervisor of André Soares
Universidade de Coimbra, Portugal
2010 - 2014 New retroviral-like membrane-associated aspartic proteinases from Rickettsiae: Biochemical characterization and specificity profiling
Supervisor of Rui Gonçalo Batista Mamede da Cruz
Universidade de Coimbra, Portugal
2012 - 2013 A Chloroplastidial Atypical Aspartic Protease from Arabidopsis thaliana: Optimization of heterologous expression, Purification and Biochemical characterization
Supervisor of Daniela Patrícia Martins Dias Pedroso
Universidade de Coimbra, Portugal
2012 - 2013 Bacterial Retropepsin-Like Proteases: The Evidence from Legionella pneumophila
Supervisor of Paulo Alexandre Gonçalves Teixeira
Universidade de Coimbra, Portugal
2010 - 2011 Heterologous overexpressionand purification of the membrane retroviral-like aspartic proteinase homologue from Rickettsia conorii
Supervisor of Liliana Freitas Antunes
Universidade de Coimbra, Portugal
2010 - 2011 Production of Phytase(s) in Chlamydomonas reinhardtii: a proof of concept study
Supervisor of Pedro Tiago Cardoso Curto
Universidade de Coimbra, Portugal
2010 - 2011 Production and characterization of recombinant shewasin D, a pepsin homologue from “Shewanella denitrificans
Supervisor of Ana Rita Leal
Universidade de Coimbra, Portugal
2009 - 2010 Insights on the interaction between Constitutive Disease Susceptibility 1A, an atypical plant aspartic proteinase, and its putative partner NADPH-dependent Thioredoxin Reductase A
Supervisor of Sofia Marques Ribeiro
Universidade de Coimbra, Portugal
2009 - 2010 Intracellular localization of Promotion of Cell Survival 1, an Arabidopsis thaliana Aspartic Proteinase. Evidences for its targeting beyond ER
Supervisor of Rodrigo Luiz dos Santos
Universidade de Coimbra, Portugal
2009 - 2010 Optimizing an expression system to study the intracellular targeting of Chlapsin in Chlamydomonas reinhardtii
Supervisor of André Filipe Marques Soares
Universidade de Coimbra, Portugal

Event organisation

Event name
Type of event (Role)
Institution / Organization
2022 - 2022 Advanced Course "Fighting Infection", International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra (2022/03/14 - 2022/03/18) (2022) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2021 - 2021 Advanced Course "Fighting Infection", International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra (2021/03/15 - 2021/03/19)
Other (Co-organisor)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Proteomics Approaches in Life Sciences” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra (2019 - 2019)
Other (Member of the Organising Committee)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Fighting Infection” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra (2019 - 2019)
Other (Co-organisor)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Fighting Infection”, International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra (2019 - 2019)
Other (Co-organisor)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Proteomics Approaches in Life Sciences”, International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra, Cantanhede, Portugal (2019 - 2019)
Other (Co-organisor)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Organizing Committee of CNC/UC-Biotech Seminar Series (2019)
Seminar (Member of the Organising Committee)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018 - 2018 INSTRUCT Supported Training Course: From protein structure to biological function through interactomics – an integrated view, 2nd Edition (2018 - 2018)
Other (Member of the Organising Committee)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2016 - 2016 INSTRUCT Supported Training Course: From protein structure to biological function through interactomics – an integrated view (2016 - 2016)
Other (Member of the Organising Committee)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2012 - 2013 Member of the Organizing Committee of Biocant Seminar Series (2012 - 2013)
Seminar (Member of the Organising Committee)
Biocant Associação de Transferência de Tecnologia, Portugal
2011 - 2011 Advanced workshop “Expression of proteins with biotechnological interest in Pichia pastoris”. University of Azores, São Miguel, Açores (2011 - 2011)
Workshop (Co-organisor)
Universidade dos Açores, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2021 - 2021 Outreach activity: "Um mito animado"/ "An animated myth", under the scope of the Literacy project "A Saúde no Saber" /"The Health in Knowledge" https://www.youtube.com/watch?v=THx_DTXQ0W8&list=PLMYLx4Ywr9u7BmcbGUZfuu_c-D2L3sMAK&index=11
Other
2021 - 2021 Outreach activity: "O Podcast"/ "The Podcast", under the scope of the Literacy project "A Saúde no Saber" /"The Health in Knowledge" https://www.youtube.com/watch?v=t6UXbKkjgHU&list=PLMYLx4Ywr9u681Jpia-4E2lLLMam3VuGu&index=11&t=25s
Other
2021 - 2021 Outreach activity: "Article in national Journal Público", under the scope of the Literacy project "A Saúde no Saber" /"The Health in Knowledge" https://www.publico.pt/2021/12/15/ciencia/noticia/paleta-cores-biotecnologia-1988721
2019 - 2019 Science Outreach: Demonstration of how Rickettsia establishes an infection
Exhibition
European Researchers' Night
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2013 - 2019 Science Outreach: Preparation of High School students for participation in both the International Biology Olympiads (IBO) and the Olympiads Ibero-Americanas of Biology (OIAB) (Collaboration with Centro Ciência Junior- Biocant) Contributions: Mentored approximately 6 students per edition/year
Other
Biocant Associação de Transferência de Tecnologia, Portugal
2015 - 2015 Science Outreach: Science communication activities to high school students in the event Year of Science: Alcobaça 2014/15), Externato Cooperativo da Benedita. Contributions: Lecturer of the workshop “Applications of Genetic Engineering – PCR”
Seminar
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2015 - 2015 Science Outreach: Science communication activities to the general public and high school students in the event: World Biotechnology Tour, Lisbon, Portugal. Contributions: Prepared and presented the activity “Glowing Proteins”
Exhibition
World Biotechnology Tour, Lisbon Portugal
2012 - 2012 Participating member of the Accelerace BIO Program (Accelerace is one of the world’s top 10 seed accelerators), Copenhagen Bio Science Park. Representing Biocant (Cantanhede, Portugal) Projects: New rennet solutions and new dermocosmetic products. Mentor: Dr. Morten Heide.
Other
COPENHAGEN BIO SCIENCE PARK, COBIS A/S, Denmark

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2019 Role of Host MicroRNAs in Staphylococcus aureus Infection
(Thesis) Main arguer
Inês Isabel Pires Serrenho (Master)
Universidade de Coimbra, Portugal
2019 Estudos através de métodos de dinâmica molecular da cardosina B
(Thesis) Main arguer
Osvaldo Silva (Other)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2018 Tailor-made plant cell cultures for the improved production of recombinant proteins
(Thesis) Main arguer
Ana Rita Basílio Santos (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 Identification and characterization of CteG, a novel Chlamydia trachomatis type III secretion effector protein
(Thesis) Main arguer
Sara Raquel Vilela Pais (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017 Efeito de antirretrovirais na replicação e mutagénese do Vírus da Anemia Infeciosa Equina (VAIE)
(Thesis) Main arguer
Andreia Filipa de Jesus Oliveira (Master)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2015 Molecular and biochemical characterization of a rare glucokinase with a cryptic function in environmental mycobacteria
(Thesis) Main arguer
Daniela Cristina Nunes Costa (Master)
Universidade de Coimbra, Portugal
2014 Interactores de DJ-1 mutadas – Elucidações sobre a doença de Parkinson
(Thesis) Main arguer
Liliana Raquel Rodrigues Loureiro (Master)
Universidade de Aveiro, Portugal
2014 Intelligent design of color-tunes ChR2 variants for optogenetics applications
(Thesis) Main arguer
Bruno Filipe Pereira da Cruz (Master)
Universidade de Coimbra, Portugal
2013 The Plant Specific Insert (PSI) and its Molecular Role in Protein Sorting
(Thesis) Main arguer
Bruno Alexandre Teixeira Peixoto (Master)
Universidade de Coimbra, Portugal
2013 Biosynthesis of rare methylglucose lipopolysaccharides in rapidly- growing mycobacteria: characterization of a key hydrolase
(Thesis) Main arguer
Maria Mafalda Santos Costa (Master)
Universidade de Coimbra, Portugal
2013 Role of local protein synthesis in presynaptogenesis
(Thesis) Main arguer
Luís Filipe Maximino Martins (Master)
Universidade de Coimbra, Portugal
2013 Inibidores de Proteinases Aspárticas com Actividade Antimicrobiana
(Thesis) Main arguer
Sara de Almeida Handem (Master)
Universidade de Coimbra, Portugal
2012 Pollen Proteases and Allergic Disorders
(Thesis) Main arguer
Ricardo Lourenço Gaspar (Master)
Universidade de Coimbra, Portugal
2012 Design of synthetic oxygen consuming devices (OCDs) and their characterization in the cyanobacterium Synechocystis sp. PCC 6803: The F1F0 ATP synthase
(Thesis) Main arguer
Sérgio Gandra Ferreira (Master)
Universidade de Coimbra, Portugal
2012 Analysis of the antimicrobial content of amphibian skin secreetions and structural and functional characterization of a novel member of the temporin family (temporin-SHe)
(Thesis) Main arguer
Sonia Maria Costa André (Master)
Universidade de Coimbra, Portugal
2012 The role of exogenous DJ-1 in neuroprotection
(Thesis) Main arguer
Matilde Pereira Neves Melo (Master)
Universidade de Aveiro, Portugal
2012 Early molecular events in the pathology of Machado-Joseph’s disease or Spinocerebellar ataxia type 3
(Thesis) Arguer
Ricardo Jorge Ladeiro Tomé (PhD)
Universidade de Coimbra, Portugal
2012 Cardosin A Molecular Determinants and Byosynthetic Pathways
(Thesis) Main arguer
Cláudia Sofia Pereira (PhD)
Universidade do Porto, Portugal
2011 The peroxisome-mitochondira connection. Identification and characterization of novel membrane proteins shares by both organelles
(Thesis) Main arguer
Inês Gomes de Oliveira e Castro (Master)
Universidade de Coimbra, Portugal
2011 The GluN2B subunit of NMDARs and its role in glutamatergic synapses: Towards the characterization of the proteome of postsynaptic densities from wild-type and GluN2B-null cultured neurons
(Thesis) Main arguer
Pedro Daniel Rocha Rio (Master)
Universidade de Coimbra, Portugal
2010 Recombinant expression of the ECM enzymes heparanase-1 (HPSE-1) and heparanase-2 (HPSE-2) for X-ray crystallographic studies
(Thesis) Main arguer
Mário Rui Pimentel Pereira (Master)
Universidade de Coimbra, Portugal
2010 Molecular mechanisms underlying the regulation of Brain-Derived Neurotrophic Factor (BDNF) translation in dendrites
(Thesis) Main arguer
Vera Lúcia Margarido Pinheiro (Master)
Universidade de Coimbra, Portugal
2010 Characterization of trypsin inhibition by boophilin
(Thesis) Main arguer
Tatiana Barros Reis Cereja (Master)
Universidade de Aveiro, Portugal
2009 Dissecting the role of human CLASPs in the establishment and maintenance of mitotic spindle bipolarity
(Thesis) Main arguer
Andrea Margarita Afonso Serra Marques (Master)
Universidade de Coimbra, Portugal
2009 Post-translational modifications of ataxin-3, the protein involved in Machado-Joseph disease - Evidences for phosphorylation and sumoylation
(Thesis) Main arguer
Carlos Adriano Albuquerque Andrade de Matos (Master)
Universidade de Coimbra, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2008 - 2022 Nature Communications; Frontiers in Cellular and Infection Microbiology; Applied Biochemistry and Biotechnology; Bioprocess and Biosystems Engineering; BMC Microbiology; Microbiology; Tropical Medicine and Infectious Disease; Frontiers in Plant Science; International Journal of Dairy Technology;
2008 - 2022 Journal of Food Chemistry; Journal of Agricultural and Food Chemistry; Molecules; Plant and Cell Physiology; Plant Physiology; Scientific Reports; Plant Biotechnology; Microbiology

Conference scientific committee

Conference name Conference host
2022 - Current 31st Meeting of the American Society for Rickettsiology American Society for Rickettsiology
2019 - 2019 30th Meeting of the American Society for Rickettsiology

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2022 - 2022 Module "Recombinant protein expression and purification in the Advanced Course" Core Technologies @ CNC" International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Organizer and Lecturer of the module (theoretical and laboratory classes) (total contact time 8h) PhD Course Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2021 - 2021 Module "Recombinant protein expression and purification¿ in the Advanced Course ¿Core Technologies @ CNC" International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Organizer and Lecturer of the module (theoretical and laboratory classes) (total contact time 8h) PhD Course (Doutoramento) (Curso de doutoramento (conclusão de unidades curriculares)) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2020 - 2020 Module “Recombinant protein expression and purification” in the Advanced Course “Core Technologies @ CNC” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Organizer and Lecturer of the module (theoretical and laboratory classes) (total contact time 10h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Module “Recombinant protein expression and purification” in the Advanced Course “Core Technologies @ CNC” International Doctoral Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Organizer and Lecturer of the module (theoretical and laboratory classes) (total contact time 10h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Computational Biology” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Lecturer of the module “The path to new synthetic protease(s) for food and cosmetics applications: Optimizing heterologous protein secretion in Kluyveromyces lactis by manipulating protein sorting” (total contact time 1h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Fighting Infection” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Co-organizer and Lecturer (total contact time 9h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Advanced Therapies” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Lecturer of the module “Rickettsial subversion tactics to evade host innate immunity” (total contact time 1h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019 - 2019 Advanced Course “Proteomics Approaches in Life Sciences” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Co-organizer and Lecturer PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018 - 2018 INSTRUCT Supported Training Course: From protein structure to biological function through interactomics – an integrated view, 2nd Edition Contributions: Co-organizer, member of the Scientific Commission. Lecturer (total contact time: 7h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018 - 2018 Advanced Course “Fighting Infection” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra Contributions: Lecturer of the module “Rickettsiae: old vector-borne pathogens with a lot to reveal (still) on obligate intracellular lifestyle” (total contact time 1h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018 - 2018 Module Cell Regulation, M.Sc. in Cellular and Molecular Biology Department of Life Sciences, Faculty of Sciences and Technology and M.Sc. in Biomedical Research, Faculty of Medicine. Contributions: Lecturer in “Advances in Protein Expression Methods” (total contact time 2h) MSc Course (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2017 - 2017 Module Cell Regulation, M.Sc. in Cellular and Molecular Biology Department of Life Sciences, Faculty of Sciences and Technology and M.Sc. in Biomedical Research, Faculty of Medicine. Contributions: Lecturer in “Advances in Protein Expression Methods” (total contact time 2h) MSc Course (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2016 - 2016 Module “Production and purification of recombinant proteins” in the Advanced Course “CNC Core Facilities” International Doctoral Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra. Contributions: Organizer and Lecturer of the module (theoretical and laboratory classes) (total contact time 24h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2016 - 2016 INSTRUCT Supported Training Course: From protein structure to biological function through interactomics – an integrated view. Contributions: Organizer and member of the Scientific Commission PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2012 - 2012 Advanced Course “Trends in Molecular Biotechnology” International Ph.D. Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra. Contributions: Organizer and lecturer (including teaching laboratory classes) (total contact time 32h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2011 - 2011 Advanced workshop “Expression of proteins with biotechnological interest in Pichia pastoris”. University of Azores, São Miguel, Açores. Contributions: Organizer and Lecturer (theoretical and laboratory classes) (total contact time 32h) PhD Course (Doutoramento) Universidade dos Açores, Portugal
2009 - 2009 Core curricular unit: Laboratories of Molecular Biology, BSc in Biochemistry, Faculty of Sciences and Technology Contributions: Prepared and taught all classes (laboratory course) (total contact time 70h, 48 students). Course coordinator: Prof. Carlos Faro BSc Course (Licenciatura) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2008 - 2008 Tutorial pratices integrated in the M.Sc. in Biochemistry, Faculty of Sciences and Technology Contributions: Tutor of 10 students, helping them preparing and participating in the teaching process (laboratory classes; discipline Laboratories of Molecular Biology) (total contact time 8h per student) MSc Course (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2008 - 2008 Core Curricular Unit: Molecular Biology, BSc in Biochemistry, Faculty of Sciences and Technology Contributions: Course coordinator, prepared and taught all classes (total contact time 40h, 50 students) BSc Course (Licenciatura) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2008 - 2008 Core curricular unit: Laboratories of Molecular Biology, BSc in Biochemistry, Faculty of Sciences and Technology Contributions: Course coordinator, prepared and taught all classes (laboratory course) (total contact time 70h, 79 students) BSc Course (Licenciatura) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2003 - 2005 Advanced Course “From protein to gene, from gene to protein” International Doctoral Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology of Coimbra. Contributions: Organizer and lecturer (including laboratory classes) (total contact time 20h) PhD Course (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2003 - 2003 Advanced Course “From Protein to Gene. From Gene to Protein” Post-Graduated training programme of CB-University of Minho, Braga. Contributions: Teaching laboratory classes (total contact time 20h) PhD Course (Doutoramento) Universidade do Minho, Portugal
2002 - 2002 Advanced Course “From Protein to Gene. From Gene to Protein” University of Azores, Ponta Delgada, Azores. Contributions: Teaching laboratory classes (total contact time 32h) PhD Course (Doutoramento) Universidade dos Açores, Portugal

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2021 Dissemination of our scientific advances to society, under the scope of our publication: Curto P, Barro A, Almeida C, Vieira-Pires RS, Simões I. The Retropepsin-Type Protease APRc as a Novel Ig-Binding Protein and Moonlighting Immune Evasion Factor of Rickettsia. mBio. 2021 Dec 21;12(6):e0305921. doi: 10.1128/mBio.03059-21. Epub 2021 Dec 7. PMID: 34872352; PMCID: PMC8649778. Total 40 clips in national outlet newspapers, equivalent to a circulation among 879 550 persons, and with an estimated monetary value of 58 315,8 €

Interview (tv / radio show)

Program Topic
2021 - Current Under the scope of the CNC Science Outreach Project: "The Health in Knowledge”, participated in a Radio Program in Radio Universidade Coimbra (RUC)
2021 - 2021 Under the scope of the CNC Science Outreach Project: "The Health in Knowledge", participated in the Program Podcast The colors of Biotechnology

Journal scientific committee

Journal title (ISSN) Publisher
2021 - Current (Review Editor) Molecular Bacterial Pathogenesis section, Frontiers in Cellular and Infection Microbiology
2021 - 2022 Co-Editor Research Topic "Obligate Intracellular Bacteria: Evasion and Adaptative Tactics Shaping the Host-Pathogen Interface", in Frontiers in Cellular and Infection Microbiology

Mentoring / Tutoring

Topic Student name
2019 - 2021 Post-doctoral Fellow Pedro Curto
2007 - 2008 Advanced training of Laboratorial Analysis Technical Degree students from the Cantanhede High School, Portugal (Technical Scientific cooperation between BIOCANT and the referred High School). Contributions: Supervised and mentored 2 students Andreia Oliveira; Cátia Costa

Other jury / evaluation

Activity description Institution / Organization
2021 - Current Ph.D. Project Committees (Chair) Susana Costa Universidade de Coimbra, Portugal
2021 - Current Ph.D. Project Committees (Chair) Ricardo Cunha Universidade de Coimbra, Portugal
2021 - Current Ph.D. Project Committees (Chair) Beatriz Almeida Universidade de Coimbra, Portugal
2021 - Current Ph.D. Project Committees (Chair) Tiago Ôchoa Pires Universidade de Coimbra, Portugal
2021 - Current Ph.D. Project Committees (Chair) Ricardo Gonzaléz Universidade de Coimbra, Portugal
2021 - Current Ph.D. Project Committees (Examiner) Miguel Rosado Universidade de Coimbra, Portugal
2020 - 2020 Ph.D. Project Committees (Examiner) Jane Pires Dias Universidade de Coimbra, Portugal
2019 - 2019 Ph.D. Project Committees (Examiner) Inês Lopes Universidade de Coimbra, Portugal
2015 - 2015 Ph.D. Project Committees (Examiner) Mafalda Costa Universidade de Coimbra, Portugal
2012 - 2012 Ph.D. Project Committees (Examiner) Dominique Fernandes Universidade de Coimbra, Portugal
Distinctions

Award

2017 Professional Society Travel Award, American Society for Rickettsiology, "ESCCAR 2017 - International Congress on Rickettsia and other intracellular bacteria", Marseille, France
2017 Bio Enterpreneur Talent Award – Microbiotec17, Congress of Microbiology and Biotechnology
2005 Professional Society Travel Award, 4th General Meeting of the International Proteolysis Society, Quebec, Canada
2004 European Regional Innovation Awards (X-PROT Project) – 2nd Place (Team Member), European Union - Committee of the Regions
2004 Post-doctoral Fellowship
Fundação para a Ciência e a Tecnologia, Portugal
2003 Professional Society Travel Award, 3rd General Meeting of the International Proteolysis Society, Nagoya, Japan
1998 Ph.D. Fellowship
Fundação para a Ciência e a Tecnologia, Portugal

Other distinction

2004 Honorary Mention - Young Entrepreneur Award, 5th Edition, Academia dos Empreendedores - Associação Nacional de Jovens Empresários (ANJE), Portugal