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João Nuno Dias Gonçalves is a PhD student of the Biomedical Sciences Doctoral Programme (School of Medicine and Biomedical Sciences of the University of Porto — ICBAS-UP) financed through the 2023 Call for PhD Studentships of the Portuguese Foundation for Science and Technology. His thesis, entitled "In the limelight of newly synthesized fluorescent nucleoside derivatives for bioimaging and drug discovery", focuses on the design and development of novel fluorescent nucleoside analogues (FNAs) with improved photophysical and biochemical properties for research, bioimaging, and pharmacological applications. João holds a BSc in Biochemistry and a MSc in Applied Biochemistry from the University of Minho. He recently concluded his MSc at the Centre of Molecular and Environmental Biology (CBMA) of the University of Minho, earning the top grade in his dissertation project. Additionally, he has participated in three International Summer Schools, where he honed his skills in computational biochemistry and key molecular biology techniques. His research investigates newly synthesized FNAs for their bioimaging and pharmacological potential. These compounds can mimic natural nucleobase-containing molecules, thereby interacting with key cellular processes such as cell signalling and metabolism. João has gained extensive experience in microbiology techniques, molecular biology, biochemical assays, fluorescence microscopy, and computational biochemistry. The primary goal of his research is to identify promising FNAs as research, diagnostic, and therapeutic agents. João has co-authored three papers in international peer-reviewed journals and contributed to seven scientific posters presented several scientific events.
Identification

Personal identification

Full name
João Nuno Dias Gonçalves

Citation names

  • Gonçalves, João N. D.
  • João Gonçalves

Author identifiers

Ciência ID
221D-6553-AE92
ORCID iD
0000-0002-8843-3948
Researcher Id
HPC-3031-2023
Scopus Author Id
58169003900

Email addresses

  • joaogoncalves444@gmail.com (Professional)

Telephones

Mobile phone
  • 934010468 (Personal)

Addresses

  • R. Entre-Devesas 191, 4850-542, Braga, Vieira do Minho, Portugal (Personal)

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Biochemistry
  • Exact Sciences - Computer and Information Sciences - Bioinformatics

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
Spanish; Castilian Elementary (A2) Intermediate (B1) Elementary (A2) Intermediate (B1) Elementary (A2)
Education
Degree Classification
2024/01/01 - 2027/12/31
Ongoing
Biomedical Sciences Doctoral Programme (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"In the limelight of newly synthesized fluorescent nucleoside derivatives for bioimaging and drug discovery: high- throughput computational drug design and live-cell pharmacological assessments”" (THESIS/DISSERTATION)
2023/09/25 - 2023/09/29
Concluded
5th International Practical Biofilm Course and Symposium: Biofilms as Promotors of Dormancy and Antimicrobial Tolerance (Outros)
Universidade do Minho Centro de Engenharia Biológica, Portugal
2020/09 - 2023/03
Concluded
Applied Biochemistry (Mestrado)
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
"Evaluation of novel purine and pyrimidine derivatives as fluorescent tools for biological applications" (THESIS/DISSERTATION)
18 (0-20 grading scale)
2022/08 - 2022/08
Concluded
Summer School "Hands-on Advanced Methods and Techniques in Plant Science and Biotechnology" (Outros)
Aarhus Universitet Institut for Agroøkologi Flakkebjerg, Denmark
10 (0-12 grading scale)
2022/07 - 2022/07
Concluded
Summer Course "Computer-Aided Drug Design" (Outros)
Università degli Studi di Pisa Dipartimento di Farmacia, Italy
Pass
2021/01 - 2021/02
Attended
Advanced Course "Statistics in Health Sciences" (Outros)
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2017/09 - 2020/07
Concluded
Biochemistry (Licenciatura)
Universidade do Minho Escola de Ciências, Portugal
"The effects of stress in the brain at the cellular and molecular level: from benefits to neuropathologies" (THESIS/DISSERTATION)
15 (0-20 grading scale)
2016/09 - 2017/06
Concluded
First Year of the Integrated Master of Sciences in Biomedical Engineering (Outros)
Universidade do Minho Escola de Engenharia, Portugal
Affiliation

Others

Category
Host institution
Employer
2024/01/01 - 2027/12/31 PhD student Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Centro de Investigação Farmacológica e Inovação Medicamentosa, Portugal
(...)
Projects

Grant

Designation Funders
2024/01/01 - 2027/12/31 In the limelight of newly synthesized fluorescent nucleoside derivatives for bioimaging and drug discovery
2023.01949.BD
PhD Student Fellow
Universidade do Porto Centro de Investigação Farmacológica e Inovação Medicamentosa, Portugal

Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal

Universidade do Minho Centro de Química, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Conference poster
  1. Gonçalves, João N. D.; Gonçalves, Jorge M.; Rodrigues, Ligia Raquel Marona ; Coutinho, Paulo; Correia-de-Sá, Paulo; Castanheira, Elisabete M. S.; Oliveira, Rui; Dias, Alice. "Highly fluorescent 2-amino-6-cyanopurines as a new bioimaging tools for studying yeast vacuoles". Paper presented in XXVIII Encontro Internacional Galego-Português de Quimica, 2024.
  2. Gonçalves, João N. D.; Gonçalves, Jorge M.; Rodrigues, Ligia Raquel Marona ; Coutinho, Paulo; Correia-de-Sá, Paulo; Castanheira, Elisabete M. S.; Dias, Alice M.; Oliveira, Rui. Corresponding author: Gonçalves, João N. D.. "Novel fluorescent nucleobase analogue for live functional imaging of yeast vacuoles". Paper presented in V CBEDS – 5th Chemical and Biological Engineering Doctoral Symposium, 2024.
  3. Gonçalves, Jorge; Gonçalves, João N. D.; Oliveira, Rui; Paulo J. G. Coutinho; Castanheira, Elisabete M. S.; Dias, Alice. Corresponding author: Dias, Alice. "2-Amino-6-Cyanopurines as Promising Fluorescent Nucleobase Analogues: Quantum Chemical Calculations of Photophysical Properties". Paper presented in 6th Symposium on Medicinal Chemistry, 2023.
  4. Gonçalves, João N. D.; Oliveira, Rui; Dias, Alice; Paulo J. G. Coutinho. Corresponding author: Gonçalves, João N. D.. "Elucidating the Binding Interaction of 2-Amino-6-cyanopurines Against the Vacuolar-type ATPase Using Molecular Docking". Paper presented in 5th Symposium on Medicinal Chemistry, 2022.
  5. Santos, A. M. ; Gonçalves, Jorge; Sousa, L. F.; Gonçalves, João N. D.; Oliveira, Rui; Paulo J. G. Coutinho; Castanheira, Elisabete M. S.; Dias, Alice. "Highly Fluorescent Nucleobase Analogues: Synthesis, Characterization and Biological Assays of a New 2-Aminopurine Derivative". Paper presented in 5th Symposium on Medicinal Chemistry, 2022.
  6. Gonçalves, Jorge; Luís F. Sousa; Ana S. Pêra; Gonçalves, João N. D.; Oliveira, Rui; Coutinho, Paulo; Castanheira, Elisabete M. S.; Dias, Alice. Corresponding author: Dias, Alice. "Novas 2-aminopurina-6-carboxiamidas como sondas fluorescentes: síntese, caracterização fotofísica e citotoxicidade". Paper presented in VI Encontro em Técnicas de Caracterização e Análise Química, 2022.
  7. Pêra, S.; Gonçalves, João N. D.; Gonçalves, Jorge; Oliveira, Rui; Paulo J. G. Coutinho; Castanheira, Elisabete M. S.; Dias, Alice. "Evaluation of a new 5,6-diaminopyrimidine as a biomembrane probe: synthesis, photophysical characterization and biological assays". Paper presented in 6th Meeting of Characterization and Chemical Analysis Techniques, 2022.
  8. Gonçalves, Jorge; Sousa, L. F.; Pêra, S; Gonçalves, João N. D.; Oliveira, Rui; Paulo J. G. Coutinho; Castanheira, Elisabete M. S.; Dias, Alice. "New 2-aminopurine-6-carboxamides as fluorescent probes: synthesis, photophysical characterization and cytotoxicity". Paper presented in 6th Meeting of Characterization and Chemical Analysis Techniques, 2022.
  9. Pêra, S; Fitas, M; Gonçalves, João N. D.; Sousa, L. F.; Castanheira, Elisabete M. S.; Paulo J. G. Coutinho; Oliveira, Rui; Dias, Alice. "New Highly Fluorescent Nucleobase Analogues as Biological Tools: Cytotoxicity Studies". Paper presented in 7th National Medicinal Chemistry Meeting / 14th National Organic Chemistry Meeting, 2022.
Journal article
  1. Jorge M. Gonçalves; João N. D. Gonçalves; Luís F. Sousa; Lígia R. Rodrigues; Paulo Correia-de-Sá; Paulo J. G. Coutinho; Elisabete M. S. Castanheira; Rui Oliveira; Alice M. Dias. "2,4,5-Triaminopyrimidines as blue fluorescent probes for cell viability monitoring: synthesis, photophysical properties, and microscopy applications". Organic & Biomolecular Chemistry (2024): https://doi.org/10.1039/D4OB00092G.
    10.1039/D4OB00092G
  2. Gonçalves, Jorge; Gonçalves, João N. D.; Pêra, A. S.; Senhorães, Nádia; Rodrigues, A. R. O.; Oliveira, Rui; Paulo J. G. Coutinho; Castanheira, Elisabete M. S.; Dias, Alice. Corresponding author: Dias, Alice. "Highly Fluorescent 2-Aminopurine Derivatives: Synthesis, Photophysical Characterization, and Preliminary Cytotoxicity Evaluation". | European Journal or Organic Chemistry (2023): https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/ejoc.202300176.
    Published • https://doi.org/10.1002/ejoc.202300176
  3. Laranjeira, Inês Martins; Gonçalves, João N. D.; Gonçalves, Cátia; Silva, Marlene; Mouta, Nuno; Dias, Alberto C.P.; Pinto-Ribeiro, Filipa. Corresponding author: Pinto-Ribeiro, Filipa. "Anti-inflammatory effect of Pterospartum tridentatum leaf extract in acute and chronic inflammation". Applied Sciences 13 7 (2023): https://www.mdpi.com/2076-3417/13/7/4494.
    Open access • Published • 10.3390/app13074494
Activities

Event participation

Activity description
Type of event
Event name
Institution / Organization
2023/12/18 - Current 3D-BIOINFO-PT Annual Meeting of The Portuguese Community of Structural Bioinformatics Researchers
Meeting
3D-BIOINFO-PT Annual Meeting
Universidade de Coimbra, Portugal
2022/11/17 - 2022/11/17 5th Symposium in Medicinal Chemistry
Symposium
5th Symposium in Medicinal Chemistry
Universidade do Minho, Portugal
2022/09/29 - 2022/09/29 Molecular dynamics simulations: applications in biomolecules
Workshop
4th Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2022/09/29 - 2022/09/29 Isolation and prospection of microorganisms from marine environment samples
Workshop
4th Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2022/09/29 - 2022/09/29 4th Symposium in Applied Biochemistry
Symposium
4th Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/10/13 - 2021/10/13 Webinar "From Large Cells to Nuclei: Key Considerations for Everyday Sorting Applications"
Other
Labroots
2021/10/08 - 2021/10/08 An hour of Python
Workshop
3rd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/10/08 - 2021/10/08 The science and not-so-science of drug development and design
Workshop
3rd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/10/08 - 2021/10/08 Scientific illustration - software, databases, and tips
Workshop
3rd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/10/07 - 2021/10/08 3rd Symposium in Applied Biochemistry
Symposium
3rd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/10/07 - 2021/10/07 Animal Experimentation - What can we do? How can we do it? And how do we feel about it?
Workshop
3rd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/10/07 - 2021/10/07 Data Analysis
Workshop
3rd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/10/07 - 2021/10/07 Scientific papers - the do's and don'ts
Workshop
3rd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2021/08/10 - 2021/08/10 Webinar "Drug Stability Testing 101: How to Ensure Your Pharmaceutical Testing Can Handle the Heat"
Other
Labroots
2021/08/09 - 2021/08/09 Webinar "Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles"
Other
Labroots
2021/07/01 - 2021/07/02 2nd International Meeting of the Portuguese Society of Genetics
Meeting
2nd International Meeting of the Portuguese Society of Genetics
2021/01/29 - 2021/01/29 2nd Symposium in Applied Biochemistry
Symposium
2nd Symposium in Applied Biochemistry
Universidade do Minho, Portugal
2020/10/29 - 2020/10/29 Webinar "Cell types to targets: Single cell RNA-sequencing for drug discovery"
Other
Labroots

Committee member

Activity description
Role
Institution / Organization
2021/02 - 2022/02 Member of the organizing committee of the 3rd Symposium in Applied Biochemistry, particularly in the technology department.
Member
Distinctions

Other distinction

2023 Top grade MSc dissertation (grade of 20 in a 0-20 grading scale)
Universidade do Minho, Portugal