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Biochemist from NOVA University Lisbon, graduated in 2014. MSc in Biotechnology from Técnico Lisboa (Portugal) in 2016. PhD in Molecular Biosciences from ITQB NOVA (Portugal) in 2022, working at iBET. From 2015 to 2022, researcher at the Cell-based Vaccines Development Lab at iBET, focusing on bioprocess intensification and evolutionary engineering strategies to enhance the production of vaccine candidates targeting infectious diseases such as COVID-19, malaria, and influenza. Author of 10 scientific manuscripts in peer-reviewed journals (including a book chapter), supervised 2 Bsc students and 3 MSc students from national and international universities, made 3 oral communications and >10 poster communications. Since 2022, Senior Scientist at the Bayer Satellite Lab at iBET, managing the Protein Production and Purification Team. Current research focuses on biomanufacturing and process development of complex biopharmaceuticals (antibodies, proteins, and viruses).
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Identification

Personal identification

Full name
Bárbara Dias Fernandes

Citation names

  • Fernandes, Bárbara

Author identifiers

Ciência ID
1A16-4C80-B2F2
ORCID iD
0000-0003-1964-1599
Scopus Author Id
57197729778

Email addresses

  • bfernandes@ibet.pt (Professional)
  • barbaradiasfernandes@gmail.com (Personal)

Telephones

Telephone
  • 214469444 (Professional)

Addresses

  • Instituto de Tecnologia Química e Biológica António Xavier (ITQB-NOVA); Instituto de Biologia Experimental e Tecnológica (iBET). Av. República, Qta. do Marquês, 2780-157, Oeiras, Oeiras, Portugal (Professional)

Websites

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology
  • Engineering and Technology - Chemical Engineering
  • Engineering and Technology - Industrial Biotechnology - Bioprocessing Technologies
  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Beginner (A1) Intermediate (B1) Beginner (A1) Beginner (A1)
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2019/01/01 - 2022/04/30
Concluded
 Ciências da Engenharia e Tecnologia (Doutoramento)
Major in Vacinas
Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
"Bioprocess Engineering of Insect Cells for Accelarating Vacines Development" (THESIS/DISSERTATION)
 Approved unanimously
2016
Concluded
 Biotecnologia (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Flexible insect cell platforms for fast production of pseudotyped virus-like particles" (THESIS/DISSERTATION)
 16
2014
Concluded
 Bioquímica (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"n.a." (THESIS/DISSERTATION)
 14
Affiliation

Science

Category
Host institution 		Employer
2017/01/01 - Current Researcher (Research) Instituto de Biologia Experimental e Tecnológica, Portugal
iNOVA4Health, Portugal
2016 - 2019 Researcher (Research) Instituto de Biologia Experimental e Tecnológica, Portugal
Instituto de Biologia Experimental e Tecnológica, Portugal

Others

Category
Host institution 		Employer
2022/01/01 - Current Senior Research Associate Bayer Satellite Lab Instituto de Biologia Experimental e Tecnológica, Portugal
2019/01/01 - 2022/04/29 PhD Student Instituto de Biologia Experimental e Tecnológica, Portugal
Instituto de Biologia Experimental e Tecnológica, Portugal
Projects

Grant

Designation Funders
2019/01 - 2022/12 Bioprocess Engineering of Insect Cells for Accelerating Vaccines Development
SFRH/BD/138937/2018
 Fundação para a Ciência e a Tecnologia
2015 - 2020 Next Generation Seasonal Influenza Vaccines: Towards a VLP-based Production Platform
IF/01704/2014/CP1229/CT0001
Research Fellow
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2017 - 2021 TRANSVAC-DS - Design Study for a European Vaccine Infrastructure
951668
Researcher
2017 - 2021 TRANSVAC2
228403
Researcher
Outputs

Publications

Book chapter
  1. Correia, Ricardo; Fernandes, Bárbara; M. Alves, Paula; Roldão, António. "Adaptive Laboratory Evolution to Improve Recombinant Protein Production Using Insect Cells". In Methods in Molecular Biology, 79-90. Springer US, 2024.
    10.1007/978-1-0716-3961-0_6
Conference poster
  1. Correia, Ricardo; Taja Zotler; Graça, Miguel; Gorben Pijlman; Roldao, Antonio. Corresponding author: Correia, Ricardo. "Continuous Production of Influenza VLPs Using IC-BEVS: a Multi-Stage Bioreactor Approach". Paper presented in ESACT Meeting 2024, 2024.
  2. Fernandes, Bárbara; Correia, Ricardo; Inês A Isidro; Alves, Paula; Roldao, Antonio. "Insect cell-based production processes intensified via high cell density perfusion and continuous culture". Paper presented in Cell Culture Engineering XVIII, 2023, 2023.
  3. Fernandes, Bárbara; Correia, Ricardo; Cláudia Paiva; Marco Silvano; Inês A Isidro; António Roldão. Corresponding author: Fernandes, Bárbara. "Continuous high-cell-density insect cell cultures for Gag-HA VLP-based vaccine production". Paper presented in 27th ESACT Meeting 2022, 2022.
  4. Correia, Ricardo; Fernandes, Bárbara; Carrondo, Manuel; Alves, Paula; Roldao, Antonio. Corresponding author: Correia, Ricardo. "Process intensification combined with adaptive laboratory evolution enhance vlp-based vaccine candidates production in insect cells". Paper presented in Continuous Biomanufacturing V, 2022, 2022.
  5. Fernandes, Bárbara; Correia, Ricardo; João Vidigal; Manuel JT Carrondo; António Roldão. "Stable insect cell platforms for production of pseudotyped VLPs". Paper presented in 26th ESACT Meeting- Copenhagen, 2019.
  6. Correia, Ricardo; Fernandes, Bárbara; Carrondo, Manuel; Roldao, Antonio; Alves, Paula. "Adaptive Laboratory Evolution of Insect Cells for Improved Influenza HA VLPs Production". Paper presented in ESACT Meeting 2019, 2019.
  7. Fernandes, Bárbara; João Vidigal; Correia, Ricardo; Cristina Peixoto; Carrondo, Manuel; Ana P Teixeira; Roldao, Antonio; Alves, Paula. "Bioprocess engineering of insect cells for accelerating vaccines development". Paper presented in Vaccine Technology VII, 2018.
  8. Fernandes, Bárbara; Correia, Ricardo; João Vidigal; Manuel JT Carrondo; António Roldão; Paula M Alves. "Bioprocess engineering of stable insect cells for enhanced production of Gag-VLPs". Paper presented in 18th European Congress on Biotechnology- Switzerland, 2018.
Journal article
  1. Ricardo Correia; Taja Zotler; Francisco Ferraz; Bárbara Fernandes; Miguel Graça; Gorben P. Pijlman; Paula M. Alves; António Roldão. "Continuous Production of Influenza VLPs Using IC-BEVS and Multi-Stage Bioreactors". Biotechnology and Bioengineering (2025): https://doi.org/10.1002/bit.28925.
    10.1002/bit.28925
  2. João P. Mendes; Bárbara Fernandes; Earl Pineda; Shashi Kudugunti; Mike Bransby; René Gantier; Cristina Peixoto; et al. "AAV process intensification by perfusion bioreaction and integrated clarification". Frontiers in Bioengineering and Biotechnology (2022): http://dx.doi.org/10.3389/fbioe.2022.1020174.
    10.3389/fbioe.2022.1020174
  3. Ricardo Correia; Bárbara Fernandes; Rute Castro; Hikaru Nagaoka; Eizo Takashima; Takafumi Tsuboi; Akihisa Fukushima; et al. "Asexual Blood-Stage Malaria Vaccine Candidate PfRipr5: Enhanced Production in Insect Cells". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.908509.
    10.3389/fbioe.2022.908509
  4. Bárbara Fernandes; Ricardo Correia; Paula M. Alves; António Roldão. "Intensifying Continuous Production of Gag-HA VLPs at High Cell Density Using Stable Insect Cells Adapted to Low Culture Temperature". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.917746.
    10.3389/fbioe.2022.917746
  5. Bárbara Fernandes; Marcos Sousa; Rute Castro; Anja Schäfer; Julia Hauser; Kai Schulze; Mario Amacker; et al. "Scalable Process for High-Yield Production of PfCyRPA Using Insect Cells for Inclusion in a Malaria Virosome-Based Vaccine Candidate". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.879078.
    10.3389/fbioe.2022.879078
  6. Bárbara Fernandes; Rute Castro; Farien Bhoelan; Denzel Bemelman; Gomes, R.A.; Julia Costa; Patrícia Gomes-Alves; et al. "Insect Cells for High-Yield Production of SARS-CoV-2 Spike Protein: Building a Virosome-Based COVID-19 Vaccine Candidate". Pharmaceutics (2022): https://www.mdpi.com/1999-4923/14/4/854.
    10.3390/pharmaceutics14040854
  7. Bárbara Fernandes; Ricardo Correia; Marcos Sousa; Manuel J. T. Carrondo; Paula M. Alves; António Roldão. "Integrating high cell density cultures with adapted laboratory evolution for improved Gag-HA virus-like particles production in stable insect cell lines". Biotechnology and Bioengineering (2021): https://doi.org/10.1002/bit.27766.
    10.1002/bit.27766
  8. Fernandes, Bárbara. "Improving Influenza HA-Vlps Production in Insect High Five Cells via Adaptive Laboratory Evolution". Vaccines 8 4 (2020): 589-589. http://dx.doi.org/10.3390/vaccines8040589.
    10.3390/vaccines8040589
  9. Fernandes, Bárbara. "Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production". Journal of Biotechnology (2020): http://dx.doi.org/10.1016/j.jbiotec.2019.10.004.
    10.1016/j.jbiotec.2019.10.004
  10. Vidigal, J.; Fernandes, B.; Dias, M.M.; Patrone, M.; Roldão, A.; Carrondo, M.J.T.; Alves, P.M.; Teixeira, A.P.. "RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles". Applied Microbiology and Biotechnology (2017): 1-12. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034256685&partnerID=MN8TOARS.
    10.1007/s00253-017-8628-3
Thesis / Dissertation
  1. "Bioprocess Engineering of Insect Cells for Accelarating Vacines Development". PhD, 2022. http://hdl.handle.net/10362/141656.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/11/13 Improving the production of biologics using insect cells 15th Annual PEGS Europe 2023
(Lisboa, Portugal)
2022/06/16 Production of high-quality SARS-CoV-2 antigens for vaccine development and serological assays implementation Vaccine Technology VIII
(Sitges, Spain)
2018/10/19 Bioprocess engineering of insect cells for enhanced Gag-VLPs production 2nd ESACT Frontiers Retreat
(Zagreb, Croatia)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/03/01 - 2022/01/01 Bioprocess engineering strategies for improve VLP-based vaccines production
Supervisor
Università degli Studi di Padova, Italy
2017/09 - 2018/11 Optimizing the production of zika and influenza virus-like-particles in insect cells
Supervisor
 Biotecnologia (Master)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2018/04 - 2018/08 Bioprocess engineering of insect cells for enhanced production of influenza vlps
Supervisor
 Assistant Engineer in Biology – Biochemistry - Biotechnologies (Master)
School D'ingénieurs En Biotechnologies De L'ucly, France

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2022/06/26 - 2022/06/29 27th ESACT Meeting, 2022, Lisbon, Portugal (2022/06/26 - 2022/06/29)
Conference (Member of the Organising Committee)
2014 - 2014 Bioengineering Week (2014 - 2014)
Meeting (Member of the Organising Committee)
 Universidade de Lisboa Instituto Superior Técnico, Portugal

Association member

Society Organization name Role
2016/01/01 - Current European Society for Animal Cell Technology

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2024/01/31 - 2024/02/01 Leadership Course for Young PIs 2024, LS4Future, Chaperone Leadership for Young Group Leaders
2020/12/17 - 2020/12/22 Science Communication Universidade Nova de Lisboa, Portugal
2017/09 - 2017/09 Cell Culture-Based Viral Vaccines Course, Llafranc, Girona, Spain Cell Culture-Based Viral Vaccines Course European Society of Animal Cell Technology, Germany
Distinctions

Award

2019 PhD grant
Fundação para a Ciência e a Tecnologia, Portugal
2017 Poster Prize at the 25th ESACT Meeting, Lausanne, Switzerland