Bárbara Gonçalves (151C-B38D-FDA2)

Curriculum Vitae

Bárbara Gonçalves

Scientific activity area i) Microbiology and Molecular Biology of Gram-positive pathogens. (ii) Antibiotic Resistance mechanisms in Gram-positive Bacteria. (iii) Bacterial interaction with the host. (iv) Characterization of the cell wall biosynthesis pathway. (v) identification of new targets for the design of antimicrobial drugs. (vi ) Bacterial identification and microplastic identification in aquaculture water Experience in: 1) Microbiology • Water analysis: Microbiology analysis of aquaculture water (CFUs, bacteria isolation and identification); • Antibiotic and Copper susceptibility assay: disk diffusion method and Agar and Broth dilution methods for Minimum Inhibitory Concentration determination- CLSI guidance; • Escherichia coli, Enterococcus and Staphycoloccus aureus manipulation: Growth monitoring in aerobic, microaerobic and anaerobic conditions. Bacterial viability analysis; • Biofilm formation assays with E. coli and S. aureus strains; • Extraction, purification and analysis of several cell wall S. aureus components: peptidoglycan (PG), Lipid II, PG O-acetylation and teichoic acids. 2) Molecular Biology • DNA cloning methods: classic cloning and site-directed mutagenesis to construct several E. coli and S. aureus mutants; • DNA transfer methods: E. coli transformation (thermal shock) and S. aureus transformation (electroporation and transduction with several phages); • RNA extraction and RT-PCR; • 16s ribosomal DNA amplification for bacteria identification. 3) Biochemistry Protein analysis: • Expression and purification of recombinant proteins by affinity, anionic and exclusion chromatography (manual or automatic columns (AKTA); • Analysis of protein expression by Western blotting; • Protein structure and protein-protein interactions by nuclear magnetic resonance (NMR); • Assessment of protein stability by Thermofluor assay; • Kinetic activity using a metalloprotein from S. aureus and Enterococcus faecium. Other analysis: • Analysis of peptidoglycan using High Performance Liquid Chromatography (HPLC); • Analysis of the presence of microplastic and macroplastic in aquaculture water by Fourier Transform Infrared Spectroscopy (FTIR). 4) Cell line manipulation • In vitro infection assays using human and mouse macrophages cell lines (THP-1 and RAW264.7) and Staphylococcus aureus wild-type strains and mutants (oatA and murT-gatD mutant); • Quantifying cellular cytotoxicity assay using LDH Cytotoxicity assay kit 5) Biofinformatic . Phylogenetic tree based on 16s rRNA sequence . Statistic

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Identification

Personal identification

Full name: Bárbara Gonçalves

Citation names

Gonçalves, Bárbara
Gonçalves, B.V.
Gonçalves, Bárbara V.

Author identifiers

Ciência ID: 151C-B38D-FDA2
ORCID iD: 0000-0001-8391-4825

Knowledge fields

Natural sciences - Biological Sciences - Microbiology

Languages

Language	Speaking	Reading	Writing	Listening
Portuguese	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)
English	Intermediate (B1)	Advanced (C1)	Intermediate (B1)	Advanced (C1)
Italian	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)

Education

	Degree	Classification
2018/10/01 - 2022/12/20 Concluded	Biologia (Doutoramento) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal "A new target inside an old molecule: glutamate amidation of peptidoglycan" (THESIS/DISSERTATION)
2014/12/15 Concluded	Mestrado em Genética Molecular e Biomedicina (Mestrado) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal "A new antimicrobial target in Staphylococcus aureus- amidation of peptidoglycan" (THESIS/DISSERTATION)	17
2012/06/27 Concluded	Licenciatura em Biologia (Licenciatura) Universidade de Évora, Portugal "n/a" (THESIS/DISSERTATION)	16

Affiliation

Others

	Category Host institution	Employer
2022/07/01 - Current	Research student-BI fellowship from Universidade de Aveiro of the project DIGIAqua, under the suepervicer of Carlos Marques, Susana Gaudêncio, Rita Sobral.	Universidade de Aveiro Departamento de Física, Portugal

2018/01/01 - 2022/01/01	phD student	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/03/01 - 2017/07/31	Research student-BI fellowship from LAQV-UCIBIO, entitled " Copper tolerance in Enterococcus faecium: unveiling the activity of a multicopper oxidase encoded by cueO gene and its gene transfer to Sta	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/07/01 - 2017/02/28	Research student-BI fellowship from the Unidade de Investigação UCIBIO/ NOVA.ID.FCT (UCIBIO/ Ciências Biológicas – Biologia Microbiana/04378)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

2016/02/01 - 2016/04/30	Research student-BI fellowship from the project "Applied Molecular Biosciences Unit” ("UCIBIO/ Ciências Biológicas – Biologia Microbiana/04378).	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

2014/10/01 - 2015/12/31	Research student-BI fellowship from the project "Amidation of the peptidoglycan of Gram-positive bacteria: an unexplored potential target for antibiotics" (PTDC/BIA-MIC/3195/2012).	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

2013/09/02 - 2014/12/15	Master student. Dissertation entitled "A new antimicrobial target in Staphylococcus aureus – amidation of peptidoglycan".	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

2011/02/01 - 2011/07/01	Programa ERASMUS.	Universidade de Évora, Portugal

Projects

Grant

	Designation	Funders
2022/07/01 - Current	Project DIGIAqua - PTDC/EEI-EEE/0415/2021 PTDC/EEI-EEE/0415/2021 Research Fellow Universidade de Aveiro, Portugal	Fundação para a Ciência e a Tecnologia
2017/01/01 - 2017/12/31	Copper tolerance in Enterococcus faecium: unveiling the activity of a multicopper oxidase encoded by cueO gene and its gene transfer to Staphylococcus aureus. PTDC/BIA-PRO/098882/2008 Research Fellow Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2014/10/01 - 2015/12/31	Amidation of the peptidoglycan of Gram-positive bacteria: an unexplored potential target for antibiotics PTDC/BIA- MIC/3195/2012 Research Fellow Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2018/10/01 - 2021/09/30	Amidação do Peptidoglicano - um alvo versátil para o sucesso no combate a Staphylococcus aureus Fighting Staphylococcus aureus - Peptidoglycan amidation as a new target PTDC/BIA-MIC/31645/2017 PhD Student Fellow Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2019/01/01 - 2019/12/31	Unidade de Ciências Biomoleculares Aplicadas UID/Multi/04378/2019 Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2015/11/01 - 2019/06/30	Microfluidics Liquid Crystal Based Bifuncional Bacterial Infeccion Sensor PTDC/FIS-NAN/0117/2014 Instituto de Nanoestruturas Nanomodelação e Nanofabricação, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Other

	Designation	Funders
2019/09 - Current	StaphOUT - Fighting Staphylococcus aureus - Peptidoglycan amidation as a new target PTDC/BIA-MIC/31645/2017 Researcher Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2023/09/01 - 2025/12/31	Institute for Health and Bioeconomy LA/P/0140/2020 10.54499/LA/P/0140/2020 Post-doc Fellow Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal Universidade NOVA de Lisboa, Portugal INESC Microsistemas e Nanotecnologias, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2023/09/01 - 2024/12/31	Applied Molecular Biosciences Unit UIDB/04378/2020 10.54499/UIDB/04378/2020 Post-doc Fellow Universidade do Porto, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade NOVA de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2023/09/01 - 2024/12/31	Applied Molecular Biosciences Unit UIDP/04378/2020 10.54499/UIDP/04378/2020 Post-doc Fellow Universidade NOVA de Lisboa, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal Universidade do Porto, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2020 - 2021	A novel Fe/S centre biogenesis system dedicated to anaerobic bacteria Pessoa 2020-2021 Researcher Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2016/07/01 - 2019/06/30	Microfluidics Liquid Crystal Based Bifunctional Bacterial Infection Sensor PTDC/FIS/NAN/0117/2014 Researcher Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016/01 - 2016/12/31	A new target inside an old molecule: glutamate amidation of peptidoglycan European Society of Clinical Microbiology and Infectious Diseases - Research Grant 2015 Researcher Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Conference abstract	Gonçalves, B.V.; D. Barreiro; R.G. Sobral; S.R. Pauleta. "The role of the multicopper oxidase in copper homeostasis in gram positive pathogens". Paper presented in Congress of Microbiology and Biotechnology, Coimbra, 2019. Gonçalves, B.V.; D. Barreiro; S.R. Pauleta; R.G. Sobral. "Dealing with copper stress - multicopper oxidases in pathogenic Gram-positive bacteria". Paper presented in XXVI Encontro Nacional da Sociedade Portuguesa de Química, Porto, 2019.
Conference poster	Gonçalves, B.V.; G. Cavaco; H. de Lencastre; L.J. Mota; R.G. Sobral. "The role of peptidoglycan amidation in host immune response.". Paper presented in ECCMID, 2022. Cavaco, Gonçalo; A.C. Fanico; N. Faria; B.V. Goncalves; M. Miragaia; R.G. Sobral. "New factors of lysozyme resistance in Staphylococcus aureus associated with peptidoglycan amidation.". Paper presented in 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 2022. Gonçalves, B.V.; G. Cavaco; H. de Lencastre; L.J. Mota; R.G. Sobral. "Establishment of the MurT-GatD complex as a new virulent factor". Paper presented in Microbiotec 2021, 2021. Cavaco, Gonçalo; B.V. Gonçalves; I.R. Grilo; H. de Lencastre; M. Miragaia; R.G. Sobral. "Peptidoglycan amidation impact on biofilm formation - role of eDNA and ATL.". Paper presented in Microbiotec 2021, 2021. Silva, M; R. Portela; B.V. Gonçalves; J.S. Dias; R.G. Sobral. "Role of ScwdABC system of Staphylococcus aureus in Biofilm formation and infection". Paper presented in Microbiotec 2021, 2021. Portela, Raquel; R. Oliveira; A. Tostão; B.V. Gonçalves; R.G. Sobral; S.R. Pauleta. "Detoxification of toxic intermediates produced by oxygen reduction in human pathogens". Paper presented in MicroBioTec, 2021. Barreiro, Daniela S.; R. Oliveira; B.V. Gonçalves; R.G. Sobral; S.R. Pauleta. "Copper proteins from pathogenic bacteria - metal homeostasis and denitrification.". Paper presented in 3rd International Workshop on Chemistry of Group 11 Elements. Caparica, Portugal. 30-31 January, 2020., 2020. Barreiro, Daniela S.; R. Oliveira; B.V. Gonçalves; R.G. Sobral; S.R. Pauleta. "Copper Proteins form Pathogenic Bacteria - Metal Homeostasis and Denitrification". Paper presented in International Workshop on Chemistry of Group 11 Elements, 2020. Gonçalves, B.V.; R. Portela; R. Lobo; T.A. Figueiredo; I.R. Grilo; A.M. Ludovice; H. de Lencastre ; J.S. Dias; R.G. Sobral. "Zooming in on MurT C-terminal domain - impact of specific residues on peptidoglycan amidation in Staphylococcus aureus.". Paper presented in The Great Wall Symposium, Paris, France, 2019. Gonçalo, Cavaco; B.V. Gonçalves; A.M. Ludovice; H. de Lencastre; R.G. Sobral. "Structural modifications in the peptidoglycan influence the autolytic system of Staphylococcus aureus.". Paper presented in The Great Wall Symposium, Paris, France, 2019. Gonçalves, B.V.; D. Barreiro; R.G. Sobral; S.R. Pauleta. "Dealing with copper stress - multicopper oxidases in pathogenic Gram-positive bacteria.". Paper presented in XXVI Encontro Nacional da SPQ. Porto, Portugal. 24 - 26 July, 2019., 2019. Gonçalves, B.V.; D. Barreiro; R.G. Sobral; S.R. Pauleta. "Dealing with copper stress in pathogenic Gram-positive bacteria.". Paper presented in Gordon Research Conference in Cell Biology of Metals. Castelldefels, Spain. 14-19 July, 2019., 2019. Gonçalves, B.V.; R. Portela; R. Lobo; T.A. Figueiredo; I.R. Grilo; A.M. Ludovice; H. de Lencastre; J.S.Dias; R.G. Sobral. "Detailed view of MurT C-terminal domain - consequences on peptidoglycan amidation in Staphylococcus aureus.". Paper presented in NOVAhealth IV Genetics Workshop, Lisboa, Portugal, 2019. Gonçalves, B.V.; C. Carreira; C. Aubert; R.G. Sobral; S.R. Pauleta. "Metalloproteins involved in copper homeostasis of Gram+ bacteria and the ORP System from strict anaerobic bacteria.". Paper presented in 2d FeSBioNet Training School, Grenoble, France, 2018. Gonçalves, B.V.; R. Portela; R. Lobo; T.A. Figueiredo; I.R.Grilo; A.M. Ludovice; H. de Lencastre; J.S. Dias; R.G. Sobral. "Essential core residues of MurT-GatD complex involved in peptidoglycan amidation of Staphylococcus aureus.". Paper presented in 18th International Symposium on Staphylococci and Staphylococcal Infections- ISSSI, Copenhagen, Denmark., 2018. Gonçalves, B.V.; R. Portela; R. Lobo; T.A. Figueiredo; I.R.Grilo; A.M.Ludovice; H. de Lencastre; J.S. Dias; R.G. Sobral. "A new target inside an old molecule: glutamate amidation of peptidoglycan.". Paper presented in Encontro Ciência 2018, Lisboa, Portugal, 2018. Gonçalves, B.V.; R.G. Sobral; S.R. Pauleta. "Unveiling the role of multicopper oxidases in pathogenic Gram positive bacteria.". Paper presented in Microbiotec17, Porto, Portugal, 2017. Gonçalves, B.V.; R.G. Sobral; S.R. Pauleta. "Unveiling the activity of Staphylococcus aureus multicopper oxidases.". Paper presented in III Genetics Workshop, Lisboa, Portugal, 2017. Gonçalves, B.V.; T.A. Figueiredo; A.M. Ludovice; H. de Lencastre; R.G. Sobral. "A new antimicrobial target in Staphylococcus aureus - amidation of peptidoglycan.". Paper presented in 2 nd Genetics Workshop, Caparica, Portugal, 2016. Gonçalves, B.V.; A.M. Ludovice; H. de Lencastre; L.J. Mota; R.G. Sobral. "Impact of peptidoglycan amidation in host evasion. Poster 115. Congresso Nacional de Microbiologia". Paper presented in MicroBiotec ´15, Évora, Portugal, 2015.
Journal article	Oliveira, Sandro C.; Soares, Simone; Rodrigues, Andreia C. M.; Gonçalves, Bárbara V.; Soares, Amadeu M. V. M.; Santos, Nuno; Kumar, Santosh; Almeida, Pedro; Marques, Carlos. "Optical fiber immunosensors based on surface plasmon resonance for the detection of Escherichia coli". Optics Express 32 6 (2024): 10077. http://dx.doi.org/10.1364/oe.518723. Open access • Published • 10.1364/oe.518723 Selvi C Ersoy; Bárbara Gonçalves; Cavaco, Gonçalo; Adhar C Manna; Rita G. Sobral; Cynthia C Nast; Richard A Proctor; et al. "Influence of Sodium Bicarbonate on Wall Teichoic Acid Synthesis and ß-Lactam Sensitization in NaHCO3-Responsive and Nonresponsive Methicillin-Resistant Staphylococcus aureus". Microbiology Spectrum (2022): https://journals.asm.org/doi/10.1128/spectrum.03422-22?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed. Published Gonçalves, B.V.; R. Portela; R. Lobo; T.A. Figueiredo; I.R. Grilo; A.M. Ludovice; H. de Lencastre; J.S. Dias; R. G. Sobral. "Role of MurT C-Terminal Domain in the Amidation of Staphylococcus aureus Peptidoglycan". Antimicrobial Agents and Chemotherapy 63 10 (2019): http://dx.doi.org/10.1128/aac.00957-19. Published • 10.1128/aac.00957-19
Thesis / Dissertation	Gonçalves, Bárbara. "A new antimicrobial target in Staphylococcus aureus - amidation of peptidoglycan". Master, Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, 2014. http://hdl.handle.net/10362/14132.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2022/03/05	Cavaco G., B.V. Gonçalves, I.R. Grilo, H. de Lencastre, M. Miragaia, R.G. Sobral. Impact of peptidoglycan amidation on cell surface properties and biofilm formation in Staphylococcus aureus.	In ECCMID. Portugal
2016/06/02	Gonçalves, B.V., T.A. Figueiredo, A.M. Ludovice, H. De Lencastre, R.G. Sobral.Essential core residues of MurT/GatD complex for peptidoglycan amidation in Staphylococcus aureus.	XL Portuguese Genetics Conference Universidade de Coimbra (Coimbra, Portugal)
2015/05/25	Gonçalves, B.V., T.A. Figueiredo, A.M. Ludovice, H. De Lencastre, L.J. Mota, R.G. Sobral. Establishment of the MurT/GatD complex as a new virulence factor - role in host evasion.	XXXIX Portuguese Genetics Conference Universidade do Minho (Braga, Portugal)
2015/01/07	Gonçalves, B.V., A.M. Ludovice, E.J. Cabrita, R.G. Sobral.A new antimicrobial target in Staphylococcus aureus - amidation of peptidoglycan.	Departamento de Ciências da Vida (DCV) Faculdade de Ciências e Tecnologia (Caparica, Portugal)
2014/03/07	Gonçalves, B.V., A.M. Ludovice, E.J. Cabrita, R.G. Sobral. A new antimicrobial target in Staphylococcus aureus - amidation of peptidoglycan.	III Jornadas Intercalares das Dissertações Anuais dos Mestrados dos Departamentos de Química e Ciências da Vida (DCV), Faculdade de Ciências e Tecnologia (Caparica, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2020/01/04 - 2020/06	Project in Biochemistry entitle "Study of the impact of peptidoglycan amidation on biofilm formation and host interaction" Co-supervisor	Bioquímica (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/01/08 - 2019/06/04	Project in Cell and Molecular Biology entitled " Production of recombinant dispersin (DspB), a PNAG-degrading enzyme from Actinobacillus actinomycetemcomitans that destroys Staphylococcus aureus biofilms" Co-supervisor	Biologia Celular e Molecular (Other) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2015 - 2019	Participation on the Expo FCT Day (2015 - 2019) Exhibition (Member of the Organising Committee)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2023/07/05 - 2023/07/07	Ciência 2023 - Science and Tecnology in Portugal Summit Conference	Universidade de Aveiro, Portugal

Course / Discipline taught

	Academic session	Institution / Organization
2019/09/04 - 2019/09/05	Super-Resolution Microscopy Workshop. In Instituto de Medicina Molecular.	Instituto de Medicina Molecular, Portugal
2018/09/10 - 2018/09/14	2nd FeSBioNet Training School. I Hands-on Training School, that aims to provide an overview of the multidisciplinary approach for the understanding of Fe-S protein biogenesis. Fundamental knowledge in genetic manipulation, biophysical characterizations (EXAFS, SAXS), spectroscopic analyses (Mössbauer & Electron Paramagnetic Resonance) and structural studies (X-ray crystallography & Electron Microscopy) - CEA Grenoble, France.	Commissariat à l’énergie atomique et aux énergies alternatives lnstitut de Recherche Interdisciplinaire de Grenoble, France
2016/02/18 - 2016/02/18	MRI Micro-Imaging - Nuclear Magnetic Resonance Laboratory I3N/CENIMAT, FCT/NLU, under the supervision of Professor Pedro Almeida and Professora Marta Corvo.	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Mentoring / Tutoring

	Topic	Student name
2022/01/04 - 2022/05/30	Co-supervision together with Professor Rita Sobral of the project - Licenciatura em Biologia Molecular e Celular. Project entitled: “Expression studies of two Staphylococcus aureus genes involved in the response to cell wall stress	Sara Filipa Tavares Cardoso.
2020/01/04 - 2020/05/30	Co-supervision together with Professor Rita Sobral of the project - Licenciatura em Biologia Molecular e Celular. Project entitled: Estudo do impacto da amidação do peptidoglican na formação de biofilme em Staphylococcus aureus	Rita Carolina Monteiro Luzio
2019/01/03 - 2019/05/28	Co-supervision together with Professor Rita Sobral of the project - Licenciatura em Biologia Molecular e Celular. Universidade Nova de Lisboa", Project entitled: Produção de Dispersina B recombinante em E. coli	Pedro Emanuel Ferreira Peralta
2016/01/03 - 2016/03/30	Co-supervision of Research trainees: Co-supervision together with Professor Rita Sobral of the trainee Beatriz Raposo, undergraduate student of	Beatriz Raposo
2014/09/01 - 2015/09/01	Teaching assistant of Master student: Laboratory teaching assistant of undergraduate student Joana Silva, supervised by Professor Rita Sobral. Thesis entitled: "Characterization of the major autolysin of Staphylococcus aureus".	Joana Filipa Gomes da Silva

Other jury / evaluation

	Activity description	Institution / Organization
2023/06/21 - Current	Jury - Bachelor´s project in Biologia Celular e Molecular (BCM). Universidade Nova de Lisboa, of the student Rafael Ricardo da Graça Gonçalves Ajuda. Thesis entitled: Characterization of two alcohol dehydrogenases from yeasts belonging to a non conventional.	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/06/21 - Current	Jury - Bachelor´s project in Bioquímica. Universidade Nova de Lisboa and Instituto Gulbenkian de Ciência, of the student Gonçalo Rosa Fernandes. Project entitled -Interaction between defective and active prophages in Escherichia coli¿, 2022.	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Distinctions

Other distinction

2011	Bolsa SÓCRATES/ERASMUS Universidade de Évora, Portugal