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Diana Barros concluded her PhD in Molecular and Environmental Biology (specialization in Evolution, Biodiversity and Ecology) in 2019 at the Centre of Molecular and Environmental Biology (CBMA), University of Minho, Braga. Before that she has done a Graduation in Applied Biology (2007) and a Master in Molecular Genetics (2011) at the same University. During the graduation internship she had the opportunity to apply basic microbiology techniques, more specifically the isolation, culture and maintenance of aquatic fungi. The internship also allowed her to gain experience in collecting and processing samples to assess fungal activity in freshwater ecosystems, namely the evaluation of functional parameters such as leaf litter decomposition, reproduction and growth of aquatic fungi. During her master she acquired knowledge on the manipulation of pathogenic fungi in level 2 and 3 biosafety laboratories, molecular and microscopy techniques and some skills in the use of bioinformatic tools with the aim of developing molecular tools for post-transcriptional gene silencing. Her PhD, within the scope of NANOECOTOX (PTDC/AAC-AMB/121650/2010) and EMERGEMIX (PTDC/BIA-BMA/30922/2017) FCT-funded projects, involved assessing the effects of silver nanoparticles (AgNPs) on microbial decomposers (fungi and bacteria) from non-polluted and polluted streams. This was achieved by characterizing key transcript and protein signatures and by examining changes in transcript and protein expression promoted by exposure to AgNPs. Diana has attended several scientific workshops and was awarded with 1 scholarship to attend the XIX National Congress of Biochemistry (SPB2016). She published 6 articles (5 as first author) in international journals of the specialty (in Q1), presented 11 communications at international conferences (5 oral communications and 6 posters) and participated in research projects as a team member (BIOTEST, POCI/MAR/56964/2004 in 2007; Unraveling the specific involvement of the small Rho-like GTPase Cdc42 in the highly polymorphic nature of Paracoccidioides brasiliensis yeast cells, PTDC/BIA-MIC/108309/2008 from 2008-2010). Diana was also a team member of AQUAFIRE (PTDC/CTA-AMB/28936/2017) FCT-funded project which aimed to estimate the risks posed by wildfires to aquatic ecosystems and human health, and a Junior Researcher at CBMA involved in the STREAMECO (PTDC/CTA-AMB/31245/2017) FCT-funded project which goal was to predict how biodiversity, ecosystem functions and services respond to multiple stressors related to climate change (warming, drought and nutrient enrichment) in stream ecosystems. Diana developed research on antioxidant enzymatic activities, microbial decomposers (aquatic fungi and bacteria), leaf litter decomposition on aquatic ecosystems, molecular tools to assess microbial decomposers and impacts of phenanthrene and silver nanoparticles in freshwater ecosystems. Present research interests focus on targeting and assessing the impacts of global change on biodiversity and the functioning of aquatic ecosystems. Diana has also been involved in outreach and science dissemination activities for enhancing scientific literacy among young people (e.g., Ciência Andante and Verão no Campus projects). Parallelly, from 2009 till nowadays, Diana has been collaborating as an invited Assistant Professor at the School of Nursing at the University of Minho and since 2024 at the School of Sciences at the same University.
Identification

Personal identification

Full name
Diana Barros
Gender
Female

Citation names

  • Barros, Diana

Author identifiers

Ciência ID
0E1D-4DDC-083D
ORCID iD
0000-0002-1355-3830

Email addresses

  • dianabarros@bio.uminho.pt (Professional)

Addresses

  • Centro de Biologia Molecular e Ambiental (CBMA), Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Braga, Portugal (Professional)
  • Institute of Science and Innovation for Bio-Sustainability, University of Minho, Campus de Gualtar, 4710-057, Braga, Braga, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Freshwater Biology
  • Natural sciences - Biological Sciences - Ecology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Advanced (C1) Advanced (C1) Upper intermediate (B2) Intermediate (B1)
Spanish; Castilian Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2020/04/26 - 2020/05/06
Concluded
Biodiversity and Ecosystem Functioning and Services under Global Change (Outros)
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Aprovada
2012/04/01 - 2019/11/22
Concluded
Programa Doutoral em Biologia Molecular e Ambiental (Doutoramento)
Major in Evolução, Biodiversidade e Ecologia
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
"Transcriptomic and proteomic responses to silver nanoparticles in aquatic microbes" (THESIS/DISSERTATION)
Aprovada por unanimidade com Muito Bom (Unanimously approved)
2016/11/15 - 2016/12/02
Concluded
Analysis of Data from Molecular Techniques (Outros)
Universidade do Porto Centro Interdisciplinar de Investigação Marinha e Ambiental, Portugal
2015/06/15 - 2015/06/18
Concluded
Proteomics course gel based protein separation by two-dimensional gel electrophoresis and protein characterization by MALDITOFTOF (Outros)
Universidade do Porto Centro Interdisciplinar de Investigação Marinha e Ambiental, Portugal
Aprovada
2014/05/05 - 2014/05/13
Concluded
Ecotoxicology and Environmental Risk Assessment (Outros)
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
18
2012/07/16 - 2012/07/27
Concluded
Microbial Genomics (Outros)
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Aprovada
2011/07/04 - 2011/07/08
Concluded
River Restoration (Outros)
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Aprovada
2007 - 2011
Concluded
Genética Molecular (Mestrado)
Universidade do Minho, Portugal
"Study of the dimorphic fungus Paracoccidioides brasiliensis: development of molecular tools and morphology evaluation. " (THESIS/DISSERTATION)
18
2004 - 2007
Concluded
Biologia Aplicada (Licenciatura)
Universidade do Minho, Portugal
"Efeito da diversidade, do etanol e do fenantreno na actividade decompositora dos fungos aquáticos." (THESIS/DISSERTATION)
15
Affiliation

Science

Category
Host institution
Employer
2021/10/06 - 2022/05/31 Contracted Researcher (Research) Universidade do Minho Escola de Ciências, Portugal
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal

Teaching in Higher Education

Category
Host institution
Employer
2009/09 - Current Invited Assistant Professor (University Teacher) Universidade do Minho, Portugal
Universidade do Minho Escola Superior de Enfermagem, Portugal

Others

Category
Host institution
Employer
2020/07 - 2021/08 Bolseiro de investigação Universidade do Minho Escola de Ciências, Portugal
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
2019/05 - 2020/01 Ciência Andante - Monitor Universidade do Minho Escola de Ciências, Portugal
Universidade do Minho Escola de Ciências, Portugal
Projects

Grant

Designation Funders
2020/07/15 - 2021/08/14 AQUAFIRE: Integrated assessment of wildfires impacts to aquatic ecosystems and human health
POCI-01-0145-FEDER-028936
Research Fellow
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2012/04/01 - 2016/03/31 Transcriptomic and proteomic responses to silver nanoparticles in aquatic microbes
SFRH/BD/80407/2011
PhD Student Fellow
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2018/10/01 - 2022/09/30 EMERGEMIX: Impacts of mixtures of emerging contaminants in aquatic ecosystems across multi-levels of biological organization (Efeitos de mixturas de contaminantes emergentes nos ecossistemas aquáticos: uma abordagem a vários níveis de organização biológica)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal

Universidade do Algarve Centro de Investigação Marinha e Ambiental, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2021/10/06 - 2022/05/31 STREAMECO: Biodiversity and ecosystem functioning under climate change: from the gene to the stream
PTDC/CTA-AMB/31245/2017
Researcher
Fundação para a Ciência e a Tecnologia
2012 - 2015 NANOECOTOX - Impacts of metal nanoparticles to aquatic ecosystems: from community responses to cellular targets
PTDC/AAC-AMB/121650/2010
Membro da equipa
2008 - 2011 Unraveling the specific involvement of the small Rho-like GTPase Cdc42 in the highly polymorphic nature of Paracoccidioides brasiliensis yeast cells
PTDC/BIA-MIC/108309/2008
Membro da equipa
Concluded
2007/01/01 - 2007/12/14 BIOTEST-Marine biotest-systems for real time oil toxicity detection
Other
Concluded
Outputs

Publications

Journal article
  1. Barros, Diana; Pradhan, Arunava; Pascoal, Cláudia; Cássio, Fernanda. "Transcriptomics reveals the action mechanisms and cellular targets of citrate-coated silver nanoparticles in a ubiquitous aquatic fungus". Environmental Pollution 268 (2021): 115913. http://dx.doi.org/10.1016/j.envpol.2020.115913.
    10.1016/j.envpol.2020.115913
  2. Barros, Diana. "Reply to the "Letter to the editor, Proteomic responses to silver nanoparticles vary with the fungal ecotype" by Huang et al.". The Science of the total environment (2020): https://doi.org/10.1016/j.scitotenv.2020.142402.
    10.1016/j.scitotenv.2020.142402
  3. Barros, Diana. "Proteomic responses to silver nanoparticles vary with the fungal ecotype". Science of The Total Environment (2019): http://dx.doi.org/10.1016/j.scitotenv.2019.135385.
    10.1016/j.scitotenv.2019.135385
  4. Diana Barros; Arunava Pradhan; Vera M. Mendes; Bruno Manadas; Pedro M. Santos; Cláudia Pascoal; Fernanda Cássio. "Proteomics and antioxidant enzymes reveal different mechanisms of toxicity induced by ionic and nanoparticulate silver in bacteria". Environmental Science: Nano (2019): https://doi.org/10.1039/C8EN01067F.
    10.1039/C8EN01067F
  5. Diana Barros; Patrícia Oliveira; Cláudia Pascoal; Fernanda Cássio. "Ethanol and phenanthrene increase the biomass of fungal assemblages and decrease plant litter decomposition in streams". Science of The Total Environment 565 (2016): 489-495. https://doi.org/10.1016/j.scitotenv.2016.04.190.
    10.1016/j.scitotenv.2016.04.190
  6. Menino JF; Osório NS; Sturme MH; Barros D; Gomes-Alves AG; Almeida AJ; Ludovico P; et al. "Morphological heterogeneity of Paracoccidioides brasiliensis: relevance of the Rho-like GTPase PbCDC42.". (2012): http://europepmc.org/abstract/med/22493946.
    10.3109/13693786.2012.665614
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/07/08 Post-fire runoffs affect plant litter decomposition and associated microbial communities in streams 2º Congresso da Sociedade Ibérica de Ecologia (SIBECOL) e o XXI congresso da Associação Ibérica de Limnologia (AIL) em associação com o 21º Encontro nacional de ecologia da Sociedade Portuguesa de Ecologia (SPECO)
University of Aveiro (aveiro, Portugal)
2022/07/07 Effects and mechanisms of emergent contaminants in freshwater biota 2º Congresso da Sociedade Ibérica de Ecologia (SIBECOL) e o XXI congresso da Associação Ibérica de Limnologia (AIL)
University of Aveiro (aveiro, Portugal)
2022/05/17 A systems toxicology approach discloses distinct mechanisms of toxicity of silver nanoparticles in freshwater microbes with different background 9th Plant Litter Processing in Freshwaters Congress (PLPF9)
University of Minho (Braga, Portugal)
2019/07/30 Omics reveal distinct mechanisms of toxicity of nanoparticulate and ionic silver in microbes 15th European Ecological Federation (EEF) Congress
(Lisboa, Portugal)
2018/06 Proteomic approach to assess the impacts of engineered nanoparticles on freshwater fungi XIX Congress of the Iberian Society of Limnology
(Coimbra, Portugal)
2018/05/16 Proteomic responses to nanoparticulate and ionic silver in freshwater microbes with different background SETAC Europe 28th Annual Meeting
(Roma, Italy)
2017/11/22 Proteomic responses to nanoparticulate and ionic silver in fungi from metal-polluted and non-polluted streams EcotoxicoMic: 1st International Conference on Microbial Ecotoxicology
2016/12 Proteomic responses to nanoparticulate and ionic silver in aquatic fungi XIX National Congress of Biochemistry
(Guimarães, Portugal)
2016/07 Proteomic analysis of aquatic microbial responses to nanoparticulate and ionic silver XXXIII Congress of the International Society of Limnology
(Turim, Italy)
2015/05 Responses of antioxidant enzymes to nanoparticulate and ionic silver in aquatic microbes SETAC Europe 25th Annual Meeting
(Barcelona, Spain)
2013 Proteomic and antioxidant enzymatic responses to nanoparticulate and ionic silver in Pseudomonas sp. M1 Advanced Course in Genomics, Metagenomics and Bioinformatics
(Braga, Portugal)
2011/06 Effects of ethanol and phenanthrene on litter decomposition by aquatic fungal assemblages 7th Symposium for European Freshwater Sciences
(Girona, Spain)
2011/03/18 Morphological heterogeneity of Paracoccidioides brasiliensis: characterization and relevance of the Rho-like GTPase Pbcdc42 (Co-author) 26th Fungal Genetics Conference
(California, United States)
2009/11 Paracoccidioides brasiliensis yeast cells do not obey standard rules during cell growth MICROBIOTEC9: National Congress of Microbiology
(Vilamoura, Portugal)

Event organisation

Event name
Type of event (Role)
Institution / Organization
2017/09/27 - 2017/09/28 2º Workshop Conjunto do Programa Doutoral em Microbiologia Aplicada e Ambiental (PDMAA) e em Biologia Molecular e Ambiental (PDBMA) (2017/09/27 - 2017/09/28)
Symposium (Member of the Organising Committee)
Universidade do Minho, Portugal
2015/11/26 - 2015/11/26 III Simpósio do Programa Doutoral em Biologia Molecular e Ambiental (PDBMA) (2015/11/26 - 2015/11/26)
Symposium (Member of the Organising Committee)
Universidade do Minho, Portugal
2012/07/02 - 2012/07/06 XVIth Congress of the Iberian Association of Limnology (2012/07/02 - 2012/07/06)
Congress (Member of the Organising Committee)
Universidade do Minho, Portugal
2006/09/09 - 2006/09/11 VIII Jornadas Biologia Aplicada - Uma nova etapa na evolução (2006/11/09 - 2006/11/11)
Other (Member of the Organising Committee)
Universidade do Minho, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2009/09 - 2020/02 Biologia Celular e Molecular Licenciatura em Enfermagem (Licenciatura) Universidade do Minho Escola Superior de Enfermagem, Portugal
2013/06/17 - 2013/06/21 Oxidative and neurotoxic stress biomarker responses to copper in stream invertebrate shredder Allogamus ligonifer: Experimental design Ecotoxicology & Environmental Risk Assessment (3rd edition) (Pós-Graduação) Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Distinctions

Award

2016 Awarded Scholarship to attend the XIX National Congress of Biochemistry (SPB2016)
Sociedade Portuguesa de Bioquímica, Portugal