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Célia Cristina Fialho Leão. Completed the Doutoramento in Biologia in 2015/12/18 by Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia and Licenciatura in Degree in Microbiology in 2008/07 by Universidade Católica Portuguesa Escola Superior de Biotecnologia. Is Researcher in Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas and Auxiliary Researcher in Instituto Nacional de Investigação Agrária e Veterinária. Published 8 articles in journals. Has 1 section(s) of books. Organized 1 event(s). Has received 4 awards and/or honors. Participates and/or participated as PhD Student Fellow in 1 project(s), Pos-doctoral Fellow in 1 project(s) and Researcher in 1 project(s). Works in the area(s) of Agrarian Sciences with emphasis on Veterinary Sciences, Natural sciences with emphasis on Biological Sciences with emphasis on Molecular Biology, Natural sciences with emphasis on Biological Sciences with emphasis on Microbiology, Exact Sciences with emphasis on Computer and Information Sciences with emphasis on Bioinformatics and Agrarian Sciences with emphasis on Agrarian and Food Biotechnology. In their professional activities interacted with 91 collaborator(s) co-authorship of scientific papers. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Paratuberculosis; Animal disease; PCR; qPCR; WGS; NGS; SNPs; metagenomics; genetic markers; NGS; genetic selection; Microbiology (medical); food security; food-producing animals; genetic determinants; mobile genetic elements; microbiology; E. coli; Salmonella; antimicrobial resistance; Mycobacterium; molecular typing; S. aureus MRSA; Pinus pinea; cork oak; meat quality; Diagnostic; Epidemiology; .
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Identification

Personal identification

Full name
Célia Cristina Fialho Leão

Citation names

  • Leão, Célia
  • Leão, C

Author identifiers

Ciência ID
0C19-EC39-BE7C
ORCID iD
0000-0002-5782-8920

Email addresses

  • celia.leao@iniav.pt (Professional)
  • celiacfleao@gmail.com (Personal)

Telephones

Mobile phone
  • 964733873 (Personal)
Telephone
  • 214403500 Ext.: 62128 (Professional)

Addresses

  • Instituto Nacional de Investigação Agrária e Veterinária, I.P. Laboratório Nacional de Referência de Saúde Animal Av. da República, Quinta do Marquês, Edificio Principal, Piso 1 , 2780 -157, Oeiras, Oeiras, Portugal (Professional)

Websites

Knowledge fields

  • Agrarian Sciences - Veterinary Sciences
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Microbiology
  • Exact Sciences - Computer and Information Sciences - Bioinformatics
  • Agrarian Sciences - Agrarian and Food Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Advanced (C1)
Spanish; Castilian Beginner (A1) Intermediate (B1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2016/04 - 2016/06/21
Concluded
 Certificado de Competências Pedagógicas (Outros)
Instituto do Emprego e Formação Profissional, Portugal
2010/10/01 - 2015/12/18
Concluded
 Biology (Doutoramento)
Major in Specialty: Microbiology
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Molecular tools in the diagnostic and epidemiology of infections caused by members of the Mycobacterium avium Complex" (THESIS/DISSERTATION)
 Unanimously Approved
2008/07
Concluded
 Degree in Microbiology (Licenciatura)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
"." (THESIS/DISSERTATION)
 16 over 20
2004
Concluded
 Curso de Especialização em Microbiologia nivel IV (Curso de Especialização Tecnológica)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
 17 over 20
Affiliation

Science

Category
Host institution 		Employer
2019/06/15 - Current Auxiliary Researcher (Research) Instituto Nacional de Investigação Agrária e Veterinária, Portugal
2017/02/01 - 2019/06/14 Postdoc (Research) Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2013/08/01 - 2014/04/15 Research Trainee (Research) Moredun Research Institute, United Kingdom
2013/02/01 - 2013/03/01 Research Trainee (Research) Neiker Tecnalia, Spain

Others

Category
Host institution 		Employer
2020/01/01 - Current Integrated Researcher MED - Mediterranean Institute for Agriculture, Environment and Development, Portugal
2017/02/01 - 2019/12/31 Integrated Researcher Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2010/10/04 - 2015/12/18 PhD student Instituto Nacional de Investigação Agrária e Veterinária, Portugal
2007/09/17 - 2008/02/29 Development of research work Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2004/05/10 - 2004/10/22 Training period - Microbiology quality control AtralCipan, Portugal
Projects

Grant

Designation Funders
2010/10/01 - 2015/04 Molecular tools in the diagnostic and epidemiology of infections caused by members of Mycobacterium avium Complex
SFRH/BD/62469/2009
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
2011/03 - 2014/02 zoonTB - Improved NAT-based approaches to detect and discriminate animal-associated Mycobacterium tuberculosis Complex members and evaluation of the zoonotic potential of these species in Portugal
Provided by PTCRIS: 111634
Other
Fundação para a Ciência e a Tecnologia, I.P.

Contract

Designation Funders
2023/01/01 - Current SMARTgNOSTICS - Global Testing & Diagnostics Solutions for antimicrobial resistances
NA
Researcher
Ongoing
2019/06/15 - 2022/09/30 CIAinVET - Food-producing animals as reservoirs of resistance to Critically Important Antibiotics
PTDC/CVT CVT/ 28469/2017
Researcher
Instituto Nacional de Investigação Agrária e Veterinária, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing

Other

Designation Funders
2017/02/01 - 2019/06/14 SelectPinea: Development of genetic markers methodology for selected traits of interest in Pinus pinea
ALT20-03-0145-FEDER-000041
Post-doc Fellow
Ongoing
Outputs

Publications

Book chapter
  1. Botelho, Ana; Canto, Ana; Leão, Célia; Cunha, Mónica V.. "Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) Analysis of Members of the Mycobacterium tuberculosis Complex". In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, 373-389. Springer New York, 2014.
    10.1007/978-1-4939-2004-4_27
  2. Botelho, A.; Canto, A.; Leão, C.; Cunha, M.V.. "Clustered regularly interspaced short palindromic repeats (CRISPRs) analysis of members of the Mycobacterium tuberculosis complex". 373-389. 2014.
Conference paper
  1. Usié A; Mendes B; Antunes M; Leão C; Marum L; Ramos A.M. "Transcriptome Characterization of Different Tissues of Stone Pine (Pinus pinea L.): de novo Assembly.". Paper presented in The 2nd International Electronic Conference on Plant Sciences-10th Anniversary of Journal Plants., 2021.
    Published
Conference poster
  1. Ana Amaro; Célia Leão (0C19-EC39-BE7C); Vanessa Guerra; Teresa Albuquerque; Lurdes Clemente. "Co-occurrence of mcr-1 and mcr-4 genes in Escherichia coli from a healthy pig in Portugal.". Paper presented in 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 2022.
  2. Célia Leão (0C19-EC39-BE7C); Ana Amaro; Patrícia Themudo; Lurdes Clemente. "Insights into pESI-like megaplasmid of Salmonella Infantis ST32 spreading in the poultry industry.". Paper presented in Microbiotec 2021, 2021.
  3. Lurdes Clemente; Célia Leão (0C19-EC39-BE7C); Patrícia Themudo; Ana Amaro. "Overview on antimicrobial resistance of Salmonella enteritidis ST11 in the poultry industry, Portugal 2014-2020". Paper presented in Microbiotec 2021, 2021.
  4. Ana Amaro; Célia Leão (0C19-EC39-BE7C); Teresa Albuquerque; Patricia Themudo; Lurdes Clemente. "Overview on the resistance to critically important antibiotics (CIA) from food-producing animals and food". Paper presented in Microbiotec 2021, 2021.
  5. M. Ferreira; C. Leão; T. Albuquerque; L. Clemente; A. Amaro. "Antibiotic resistance profiles of Escherichia coli isolated from broilers raised under intensive and semi-intensive production systems.". Paper presented in 3rd International Conference of the European College of Veterinary Microbiology (ICECVM), 2021.
  6. Ana Amaro; Célia Leão (0C19-EC39-BE7C); Patricia Themudo; Teresa Albuquerque; Ana Botelho; Lurdes Clemente. "Dissemination of mcr-1 gene among multidrug-resistant Escherichia coli from pigs and pork, Portugal, 2016–2019". Paper presented in 31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 2021.
  7. Joana Gião; Célia Leão (0C19-EC39-BE7C); Lurdes Clemente; Ana Amaro. "Presence of poxtA and optrA genes in linezolid resistant Enterococcus faecium and Enterococcus faecalis from pigs in Portugal". Paper presented in 31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 2021.
  8. Célia Leão (0C19-EC39-BE7C); Ana Amaro; Teresa Albuquerque; Lurdes Clemente. "Linezolid-resistant cfr-positive methicillin-resistant Staphylococcus aureus CC398 from healthy pigs in Portugal.". Paper presented in 31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 2021.
  9. D. Gaspar; A. Usié; C. Leão; C. Matos; L. Padre; C. Dias; C. Ginja; A.M. Ramos. "Unveiling genomic regions that underlie footrot resistance in Portuguese sheep Merino". Paper presented in 38th International Society for Animal Genetics Conference, ISAG 2021, 2021.
  10. Amaro, Ana; Leão, Célia; Patrícia Themudo; Lurdes Clemente. "Emergence of multidrug resistant Salmonella Infantis ST32 in the poultry industry, Portugal 2016-2020.". Paper presented in 3rd Annual Scientific Meeting of the One Health European Joint Programme, 2021.
  11. Célia Leão (0C19-EC39-BE7C); Lurdes Clemente; Patrícia Themudo; Ana Botelho; Ana Amaro. "Colistin resistance in Salmonella Enteritidis ST11 in the poultry industry interface, Portugal 2014-2020.". Paper presented in 3rd Annual Scientific Meeting of the One Health European Joint Programme, 2021.
  12. Célia Leão (0C19-EC39-BE7C); Ana Amaro; Teresa Albuquerque; Lurdes Clemente. "Whole genome sequencing of Linezolid-Resistant Staphylococcus aureus ST398 from healthy pigs in Portugal". Paper presented in 3rd Annual Scientific Meeting of the One Health European Joint Programme, 2021.
  13. Gaspar, D; Magalhães, H; Usié, A; Leão, C (0C19-EC39-BE7C); Ginja, C; Matos, C; Ramos, AM. "Genomic characterization of Portuguese native sheep breeds". Paper presented in 71st Annual Meeting of European Federation of Animal Science. Virtual meeting, 2020.
  14. Joana Gião; Lurdes Clemente; Teresa Albuquerque; Célia Leão (0C19-EC39-BE7C); Ana Amaro. "Antibiotic susceptibility of Enterococcus faecium and Enterococcus faecalis strains from bovine and swine gut flora.". Paper presented in 2nd International Conference of the European College of Veterinary Microbiology (ICECVM), online event, 2020.
  15. Célia Leão (0C19-EC39-BE7C); Lurdes Clemente; Vanessa Guerra; Ana Botelho; Ana Amaro. "Genomic characterization of Escherichia coli strain co-producing CTX-M-32 and CTX-M-2.". Paper presented in 2nd International Conference of the European College of Veterinary Microbiology (ICECVM), online event, 2020.
  16. Leão, Célia; Moura, Laura; EURL Team; Ana Rosa Botelho; Clemente Lurdes; Amaro Ana. "Occurrence of blaCTX-M-65 in multidrug resistant Escherichia coli from retail meat.". Paper presented in One Health EJP Annual Scientific Meeting 2020, 2020.
  17. Ana Amaro; Leão, Célia; Vanessa Guerra; EURL-Team; Ana Rosa Botelho; Lurdes Clemente. "Identification of multidrug resistant Escherichia coli ST410 carrying blaCTX-M-15 and aac(6')Ib-cr in food producing animals.". Paper presented in One Health EJP Annual Scientific Meeting 2020, 2020.
  18. Ana Amaro; Leão, Célia; Vanessa Guerra; Laura Moura; Ivone Correia; Teresa Albuquerque; Teresa Nogueira; et al. "Plasmid-mediated resistance to critically important antibiotics in Escherichia coli isolated from food animals and retail meat.". Paper presented in One Health EJP Annual Scientific Meeting 2020, 2020.
  19. Usié, A (0514-DE42-4619); Magalhães, H; Antunes, M; Leão, C (0C19-EC39-BE7C); Gaspar, D; Meireles, B; Barbosa, P; et al. "Whole genome resequencing analysis of Alentejano pigs reveals differences associated with meat quality phenotypes.". Paper presented in 9th Bioinformatics open days, 2020.
  20. Mendes, B; Leão, C (0C19-EC39-BE7C); Antunes, M; Usié, A (0514-DE42-4619); Ramos, AM. "Characterization of the stone pine (Pinus pinea) needle transcriptome: de novo assembly and SNP identification.". Paper presented in 9th Bioinformatics open days, 2020.
  21. Usié, A (0514-DE42-4619); Magalhães, H; Leão, C (0C19-EC39-BE7C); Gaspar, D; Meireles, B; Barbosa, P; Cachucho, L; et al. "Whole Genome Analysis of Alentejano Pigs with Contrasting Meat Quality Phenotypes.". Paper presented in PAG XXVIII, 2020.
  22. Leão, C (0C19-EC39-BE7C); Usié, A (0514-DE42-4619); Antunes, M; Magalhães, H; Ramos, AM. "Identification and characterization of SNPs in the stone pine (Pinus pinea) transcriptome.". Paper presented in 8º Bioinformatics Open Days 2019, 2019.
  23. Clemente, L; Moura, L; Leão, C (0C19-EC39-BE7C); Guerra, V; Correia, I; Albuquerque, T; Themudo, P; et al. "Diversity of plasmid-mediated resistance to critically important antibiotics in Escherichia coli isolated from meat, Portugal, 2016-2017". Paper presented in SAlimentar2019, 2019.
  24. Cachucho, L; Albuquerque, A; Guerreiro, O; Alves, SP; Bessa, RJB; Usié, A (0514-DE42-4619); Gaspar, D; et al. "Fatty acids composition of Longissimus lumborum muscle from Alentejano pigs finished under free-range conditions.". Paper presented in 17th Euro Fed Lipid Congress and Expo Seville, 2019.
  25. Cachucho, L; Usié, A (0514-DE42-4619); Alburquerque, A; Leão, C (0C19-EC39-BE7C); Meireles, B; Barbosa, P; Gaspar, D; et al. "Composição da carcaça e caracterização físico-química de músculo de porco alentejano acabado em montanheira – resultados preliminares". Paper presented in XX Congresso de Zootecnia, 2018.
  26. Cachucho, L; Usié, A (0514-DE42-4619); Albuquerque, A; Leão, C (0C19-EC39-BE7C); Meireles, B; Barbosa, P; Gaspar, D; et al. "Characterization of carcass composition and meat quality traits of Alentejano pigs finished under free-range conditions - Preliminary results". Paper presented in 4th Fatty Pig Science & Utilization International Conference, 2017.
  27. Leão, C (0C19-EC39-BE7C); Botelho, A; Martins, E.; Aguiar, C; Nunes, T; Rebelo, I; Bexiga, R. "Presence of Mycobacterium avium subs. paratuberculosis DNA in milk used to feed calves in Portugal.". Paper presented in World Buiatrics Congress 2016, 2016.
  28. Leão, C (0C19-EC39-BE7C); Barbosa, P; Usié, A (0514-DE42-4619); Chaves, I; Amaro, A; Botelho, A; Pinto, C; et al. "Molecular characterization of a rare pigmented Mycobacterium avium subsp. paratuberculosis Type C strain.". Paper presented in 13th International Colloquium on Paratuberculosis, 2016.
  29. Barbosa, P; Leão, C (0C19-EC39-BE7C); Chaves, I; Amaro, A; Botelho, A; Pinto, C; Stevenson, K; Inácio, J; Ramos, AM. "Genome wide comparison between Mycobacterium avium subsp. paratuberculosis non-pigmented and pigmented type C isolates.". Paper presented in 5th Bioinformatics Open Days 2016, 2016.
  30. Reis, A; Leão, C (0C19-EC39-BE7C); Bandeira, V; Fonseca, C; Cunha, MV. "On the trail of Mycobacterium avium subsp. paratuberculosis.". Paper presented in cE3c Annual Meeting 2015, 2015.
  31. Amaro, A; Nunes, M; Gomes, J; Leão, C (0C19-EC39-BE7C); Inácio, J; Vieira, ML. "Molecular investigation of Borrelia burgdorferi sensu lato in serum of several animal species from mainland Portugal.". Paper presented in EurNegVec, 2015.
  32. Leão, C (0C19-EC39-BE7C); Amaro, A; Pinto, C; Botelho, A; Inácio, J. "Semi-nested probe-based real-time PCR for the detection of Mycobacterium avium subsp paratuberculosis in goats". Paper presented in 12th International Colloquium on Paratuberculosis, 2014.
  33. Leão, C (0C19-EC39-BE7C); Amaro, A; Botelho, A; Inácio, J. "Screening of asymptomatic Portuguese cattle for the presence of Mycobacterium avium subsp. paratuberculosis in fecal samples". Paper presented in CCV2014, 2014.
  34. Leão, C (0C19-EC39-BE7C); Amaro, A; Santos-Sanches, I; Botelho, A; Inácio, J. "Assessment of Mycobacterium avium subs. paratuberculosis faecal shedding in asymptomatic Portuguese cattle.". Paper presented in Microbiotec2013, 2013.
  35. Leão, C (0C19-EC39-BE7C); Amaro, A; Santos-Sanches, I; Botelho, A; Inácio, J. "Assessment of Mycobacterium avium subsp. paratuberculosis infection in asymptomatic Portuguese cattle using molecular and culture-based assays.". Paper presented in XVII World Buiatrics Congress 2012, 2012.
  36. Leão, C (0C19-EC39-BE7C); Inácio, J; Santos-Sanches, I; Botelho, A. "Genetic heterogeneity of Mycobacterium avium subspecies hominissuis isolated in Portugal.". Paper presented in Microbiotec 2011, 2011.
  37. Leão, C (0C19-EC39-BE7C); Inácio, J; Santos-Sanches, I; Botelho, A. "Evaluation of Mycobacterium avium tandem repeat (MATR) typing of M. avium subsp. hominissuis strains with a high diversity of IS1245-RFLP profiles". Paper presented in ESM 2011, 2011.
Journal article
  1. Gaspar, Daniel; Ginja, Catarina; Carolino, Nuno; Leão, Célia; Monteiro, Helena; Tábuas, Lino; Branco, Sandra; et al. "Genome-wide association study identifies genetic variants underlying footrot in Portuguese Merino sheep". BMC Genomics 25 1 (2024): http://dx.doi.org/10.1186/s12864-023-09844-x.
    10.1186/s12864-023-09844-x
  2. Célia Leão; Leonor Silveira; Ana Usié Chimenos; Joana Gião; Maria de Lurdes Tavares Clemente; Patricia Themudo; Ana Amaro; Angela Pista; Pista Angela; A Pista; Pista A; Â Pista; Pista Â; Ângela Pista. "Genetic Diversity of Salmonella enterica subsp. enterica Serovar Enteritidis from Human and Non-Human Sources in Portugal". Pathogens (2024): https://www.mdpi.com/2076-0817/13/2/112.
    10.3390/pathogens13020112
  3. Usié, Ana; Serra, Octávio; Barros, Pedro M.; Barbosa, Pedro; Leão, Célia; Capote, Tiago; Almeida, Tânia; et al. "An improved reference genome and first organelle genomes of Quercus suber". Tree Genetics & Genomes 19 6 (2023): http://dx.doi.org/10.1007/s11295-023-01624-8.
    10.1007/s11295-023-01624-8
  4. Usié, Ana; Leão, Célia; Gaspar, Daniel; Monteiro, Helena; Tábuas, Lino; Bettencourt, Elisa; Caetano, Pedro; et al. "A metagenomics approach to characterize the footrot microbiome in Merino sheep". Veterinary Microbiology 281 (2023): 109745. http://dx.doi.org/10.1016/j.vetmic.2023.109745.
    10.1016/j.vetmic.2023.109745
  5. Amaro, Ana; Leão, Célia; Guerra, Vanessa; Albuquerque, Teresa; Clemente, Lurdes. "Plasmid-Mediated Colistin Resistance Genes mcr-1 and mcr-4 in Multidrug-Resistant Escherichia coli Strains Isolated from a Healthy Pig in Portugal". Microbial Drug Resistance 29 3 (2023): 78-84. http://dx.doi.org/10.1089/mdr.2022.0228.
    10.1089/mdr.2022.0228
  6. Gaspar, Daniel; Usié, Ana; Leão, Célia; Guimarães, Sílvia; Pires, Ana Elisabete; Matos, Claudino; Ramos, António Marcos; Ginja, Catarina. "Genome-wide assessment of the population structure and genetic diversity of four Portuguese native sheep breeds". Frontiers in Genetics 14 (2023): http://dx.doi.org/10.3389/fgene.2023.1109490.
    Published • 10.3389/fgene.2023.1109490
  7. Leão, Célia; Clemente, Lurdes; Cara d’Anjo, Maria; Albuquerque, Teresa; Amaro, Ana. "Emergence of Cfr-Mediated Linezolid Resistance among Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) from Healthy Pigs in Portugal". Antibiotics 11 10 (2022): 1439. http://dx.doi.org/10.3390/antibiotics11101439.
    10.3390/antibiotics11101439
  8. Ferreira, Mariana; Leão, Célia; Clemente, Lurdes; Albuquerque, Teresa; Amaro, Ana. "Antibiotic Susceptibility Profiles and Resistance Mechanisms to ß-Lactams and Polymyxins of Escherichia coli from Broilers Raised under Intensive and Extensive Production Systems". Microorganisms 10 10 (2022): 2044. http://dx.doi.org/10.3390/microorganisms10102044.
    Published • 10.3390/microorganisms10102044
  9. Gião, Joana; Leão, Célia; Albuquerque, Teresa; Clemente, Lurdes; Amaro, Ana. "Antimicrobial Susceptibility of Enterococcus Isolates from Cattle and Pigs in Portugal: Linezolid Resistance Genes optrA and poxtA". Antibiotics 11 5 (2022): 615. http://dx.doi.org/10.3390/antibiotics11050615.
    Published • 10.3390/antibiotics11050615
  10. Albuquerque, Catarina; Cavaco, Sandra; Caetano, Pedro; Branco, Sandra; Monteiro, Helena; Ramos, Marcos; Usié Chimenos, Anabel; Leão, Célia; Botelho, Ana. "Ovine footrot in Southern Portugal: Detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores". Veterinary Microbiology 266 (2022): 109339. http://dx.doi.org/10.1016/j.vetmic.2022.109339.
    Published • 10.1016/j.vetmic.2022.109339
  11. Lurdes Clemente; Célia Leão; Laura Moura; Teresa Albuquerque; Ana Amaro. "Prevalence and Characterization of ESBL/AmpC Producing Escherichia coli from Fresh Meat in Portugal". Antibiotics (2021): https://www.mdpi.com/2079-6382/10/11/1333.
    10.3390/antibiotics10111333
  12. Leão, Célia; Clemente, Lurdes; Guerra, Vanessa; Botelho, Ana; Amaro, Ana. "Occurrence of a Rare Multidrug Resistant Escherichia coli Coharboring blaCTX-M-32 and blaCTX-M-2 Genes in a Bovine". Microbial Drug Resistance (2021): http://dx.doi.org/10.1089/mdr.2020.0520.
    10.1089/mdr.2020.0520
  13. Célia Leão (0C19-EC39-BE7C); Ana J. Oliveira; Catarina Cruz; Ana Amaro; Carlos Pinto; João Inácio; Ana Botelho. "Evidences of Mycobacterium avium subsp. paratuberculosis infection in ruminants from an intensive dairy production region". Revista Portuguesa de Ciências Veterinárias 116 617 (2021):
    Published
  14. Leão, Célia; Clemente, Maria; Moura, Laura; Seyfarth, Anne Mette; Hansen, Inge M.; Hendriksen, Rene S.; Amaro, Ana. "Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal". (2021): http://hdl.handle.net/10400.5/22504.
    10.3389/fmicb.2021.653595
  15. Cunha, Mónica V.; Rosalino, Luís Miguel; Leão, Célia; Bandeira, Victor; Fonseca, Carlos; Botelho, Ana; Reis, Ana C.. "Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxy". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-57679-3.
    10.1038/s41598-020-57679-3
  16. Cunha, Mónica V.; Rosalino, L. M.; Leão, Célia; Bandeira, Victor; Fonseca, Carlos; Botelho, Ana; Reis, Ana C.. "Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxy". (2020): http://hdl.handle.net/10451/46085.
    10.1038/s41598-020-57679-3
  17. Branco, Sandra; Padre, Ludovina; Dias, Clara; Albuquerque, Catarina; Leão, Célia; Cavaco, Sandra; Botelho, Ana. "GEN-RES-ALENTEJO - Caracterização bacteriológica da peeira e do parasitismo gastrointestinal em ovinos na região do Alentejo". (2019): http://hdl.handle.net/10174/27943.
  18. Barbosa, Pedro; Leão, Célia; Usié, Anabel; Amaro, Ana; Botelho, Ana; Pinto, Carlos; Inácio, João; Stevenson, Karen; Ramos, António Marcos. "Draft Genome Sequence of a Rare Pigmented Mycobacterium avium subsp. paratuberculosis Type C Strain". Genome Announcements 5 41 (2017): http://dx.doi.org/10.1128/genomea.01066-17.
    10.1128/genomea.01066-17
  19. Leão, Célia; Botelho, Ana; Martins, Elisabete; Aguiar, Carla; Rebelo, Inês; Nunes, Telmo; Bexiga, Ricardo. "Presence of Mycobacterium avium subs. paratuberculosis DNA in milk used to feed calves in Portugal". Journal of Dairy Research 84 2 (2017): 124-127. http://dx.doi.org/10.1017/s0022029917000164.
    10.1017/s0022029917000164
  20. Leão C; Goldstone RJ; Bryant J; McLuckie J; Inácio J; Smith DG; Stevenson K; et al. "Novel Single Nucleotide Polymorphism-Based Assay for Genotyping Mycobacterium avium subsp. paratuberculosis.". (2016): http://europepmc.org/abstract/med/26677250.
    10.1128/JCM.01958-15
  21. Leão, C (0C19-EC39-BE7C); Amaro, A; Santos-Sanches, I; Inácio, J; Botelho, A. "Paratuberculosis asymptomatic cattle as spillovers of Mycobacterium avium subsp. paratuberculosis: consequences for disease control.". Rev Port Ciênc Vet 110 (2015): 63-73. http://www.fmv.ulisboa.pt/spcv/PDF/pdf6_2015/69-73.pdf.
  22. Leão C; Canto A; Machado D; Sanches IS; Couto I; Viveiros M; Inácio J; Botelho A. "Relatedness of Mycobacterium avium subspecies hominissuis clinical isolates of human and porcine origins assessed by MLVA.". (2014): http://europepmc.org/abstract/med/25085520.
    10.1016/j.vetmic.2014.06.027
  23. Gomes J; Leão C; Ferreira F; Afonso MO; Santos C; Josenando T; Seixas J; Atouguia J; Centeno-Lima S. "Molecular identification of T. brucei s.l. in tsetse flies after long-term permanence in field traps.". (2009): http://europepmc.org/abstract/med/19858577.
  24. Gomes, J.; Leão, C.; Ferreira, F.; Afonso, M.O.; Santos, C.; Josenando, T.; Seixas, J.; Atouguia, J.; Centeno-Lima, S.. "Molecular identification of T. brucei s.l. in tsetse flies after long-term capture in field traps". Journal of Infection in Developing Countries 3 9 (2009): 735-738. http://www.scopus.com/inward/record.url?eid=2-s2.0-74549121001&partnerID=MN8TOARS.
    10.3855/jidc.389
Magazine article
  1. Célia Leão; João Inácio; Ana Botelho. "Highlights on Mycobacterium avium subsp. paratuberculosis: a fastidious yet insidious microorganism.", Portuguese Society for Microbiology Magazine. , 2013, https://spmicrobiologia.files.wordpress.com/2013/12/2013129-12c.pdf.
Newsletter article
  1. Amaro, Ana; Célia Leão (0C19-EC39-BE7C); Joana Gião; Lurdes Clemente. "CIAinVET Newsletters nº3", 2022, https://projects.iniav.pt/ciainvet/images/newsletter/Newsletter_3_CIAinVET.pdf.
  2. Ana Amaro; Célia Leão (0C19-EC39-BE7C); Lurdes Clemente. "Newsletter N.2 CIAinVET", Newsletter N.2 CIAinVET , 2021, https://www.iniav.pt/divulgacao/noticias-iniav/1910-newsletter-2-do-projeto-ciainvet.
  3. Joana Gião; Célia Leão (0C19-EC39-BE7C); Lurdes Clemente; Ana Amaro. "Occurrence of linezolid transferable resistance mechanisms in LA-MRSA, Enterococcus faecalis and Enterococcus faecium from pigs", NEWSLETTER to the National Reference Laboratories for Antimicrobial Resistance, 2021, https://www.eurl-ar.eu/CustomerData/Files/Folders/22-newsletter/623_2021-dec-newsletter-no15-final.pdf.
  4. Leão, Célia. "Occurrence of blaCTX-M-65 in multidrug resistant Escherichia coli from retail meat", NEWSLETTER to the National Reference Laboratories for Antimicrobial Resistance. No. 14 – December 2020, 2020, https://www.eurl-ar.eu/CustomerData/Files/Folders/22-newsletter/574_2020-dec-newsletter-no14-final.pdf.
  5. Leão, Célia. "Newsletter N.1 CIAinVET", 2020, https://projects.iniav.pt/ciainvet/index.php/divulgacao/newsletter.
  6. Cunha, MV; Leão, C (0C19-EC39-BE7C). "Estudo deteta a influência do clima e altitude na circulação do agente da paratuberculose em carnívoros silvestres do Mediterrâneo", CE3C Centre for Ecology, Evolution and Environmental Changes News, 2020, https://ce3c.ciencias.ulisboa.pt//outreach/press&events/ver.php?id=1184.
  7. Leão, Célia. "Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxy", CE3C, 2020, https://ce3c.ciencias.ulisboa.pt/research/publications/ver.php?id=1685.
  8. Leão, C; Inácio J; Albuquerque T; Botelho A. "Paratuberculose.", Boletim Informativo da Lacticoop. Lacticoop 50 anos., 2012
Thesis / Dissertation
  1. Leão, Célia. "Molecular tools in the diagnostic and epidemiology of infections caused by members of Mycobacterium avium Complex". PhD, Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, 2015. http://hdl.handle.net/10362/16513.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/09/28 Do fenótipo ao genótipo: Aplicação da genómica à resistência aos antimicrobianos (RAM) no LNR de Saúde Animal. Conferência CIAinVET
INIAV (Oeiras, Portugal)
2022/09/28 Multirresistência em Staphylococcus aureus resistentes à meticilina (MRSA) em suínos. Conferência CIAinVET
INIAV (Oeiras, Portugal)
2020/02 Characterization of genomic variation in Portuguese sheep breeds using whole genome resequencing 9th Bioinformatics open days
(Braga, Portugal)
2019/11 Whole genome sequencing analysis as a powerful tool to dissect the genomic architecture of indigenous Portuguese pig and sheep breeds. IV Encontro de Estudantes de Doutoramento em Ambiente e Agricultura – EEDAA
(Évora, Portugal)
2014/06 Novel SNP-based analysis for the differentiation of isolates of Mycobacterium avium subsp. paratuberculosis. 12th International Colloquium on Paratuberculosis
(Parma, Italy)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2022/08 - Current Collaboration in the organization of the final event of the project CIAinVET (Conferência CIAinVET) (2022/09/28 - 2022/09/28)
Conference
2017 - 2019 Colaboration in the organization of the institutional stands at OviBeja, RuralBeja, Patrimonios do Sul, Feira Nacional da Água e do Regadio (2017, 2018, 2019) for the science outreaching of CEBAL’s projects and main intervention areas. (2017 - 2019)
Exhibition (Other)
2018/05/14 - 2018/05/18 Commemoration of the 10th anniversary of CEBAL "Semana Aberta do CEBAL – Comemoração do 10º aniversário do CEBAL". Organization of scientific activities for children, visits to experimental fields, talks with researchers, technological demonstrations, technical scientific events. (2018/05/14 - 2018/05/18)
Other (Co-organisor)
 Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2020/12/18 - 2020/12/18 Protocol for whole genome sequencing and bioinformatic analysis of bacterial isolates related to the EU monitoring of antimicrobial resistance. Webinar
Seminar
2020/11/24 - 2020/11/24 Compliance to the new AMR Decision - webinar
Seminar
2020/11/03 - 2020/11/03 Sensititre webinar about the new Sensititre plate production for the EU monitoring of AMR in food
Seminar
2020/09/25 - 2020/09/25 Science meets Policy conference: Modern technologies to enable response to crises: Next Generation Sequencing to tackle food-borne diseases in the EU (Online)
Conference
2020/09/03 - 2020/09/03 Webinar for presentation of "GLASS Whole-genome sequencing for surveillance of antimicrobial resistance and the GLASS Guidance for national reference laboratories," documents
Seminar
2020/06/22 - 2020/06/25 Recomb2020 the 24th International Conference on research in computational Molecular Biology. Online event
Conference
2020/04/29 - 2020/04/29 4th joint meeting on AMR for the FWD-Network and EURL-AR Network (online event)
Seminar
2020/04/29 - 2020/04/29 OHEJP Thematic Integrative Meeting (webinar on the practical use of NGS)
Seminar

Consulting

Activity description Institution / Organization
2015/12 - 2017/01 Hygiene and Food Safety Training and Audits for employes of supermarkets and restaurants industry, with the aim of warning for the biological, chemical and physical hazards in food, a risk to the Public Health. Silliker Portugal, S. A. | Mérieux NutriSciences Portugal, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2024/01/25 - 2024/01/26 Training course "Analysis of Campylobacter Sequence data" Swedish Veterinary Agency, SVA, Sweden
2021/06/01 - 2021/06/02 Trainning course in MiSeq by Illumina (Outros) Quilaban, Portugal
2021/04/26 - 2021/04/29 Training course to ensuring harmonization in relation to the monitoring of antimicrobial resistance (AMR) in zoonotic and commensal bacteria across EU. Web-based tools to generate and use whole genome sequence (WGS) data for the future AMR monitoring and according to the proposed new EU Decision. Danmarks Tekniske Universitet, Denmark
2021/02/19 - 2021/02/19 Illumina: - Genotyping: from microsatellites to SNPs arrays: genetic disorders and disease resistance; - NGS: NGS applications dedicated to the fields of veterinary (pathogen detection) and agronomy.
2020/10/19 - 2020/10/23 Training Course (Online) administrated by the EURL-AR. The aim of this course was to prepare and strengthen the laboratories abilities to work with bacterial sequence data in relation to the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria.
2019/09/24 - 2019/09/27 Training Course in Enhancing WGS capacity for AMR surveillance in the EURL-AR network. The training course aims at ensuring harmonization on the monitoring of antimicrobial resistance (AMR) in zoonotic and commensal bacteria across EU, and in particular will provide participants with knowledge and tools to generate and use whole genome sequence (WGS) data for the future AMR monitoring and according to the proposed new EU Decision Danmarks Tekniske Universitet, Denmark
2019/07/11 - 2019/07/12 Training Course on WGS data use: bioinformatics tools for aiding STEC outbreak investigation. To consolidate the knowledge on the analysis of WGS data and to increase the level of skill and awareness within the NRL network. Istituto Superiore di Sanità, Italy

Mentoring / Tutoring

Topic Student name
2020 - 2022/02/15 Contribution in the supervision of a master student in Veterinary Medicine, in AMR of E. coli from different production systems Mariana Ferreira
2021/09 - 2022/02 Contribution in the supervision of a master student in Veterinary Medicine, in E. coli Escherichia coli from Broilers Raised under Intensive and Extensive Production Systems Mariana Ferreira
2020 - 2021/11/26 Contribution in the supervision of a master student in Veterinary Medicine, in Enterococcus faecium and faecalis AMR Joana Gião
2019 - 2020 Contribution in the supervision of a master student in Food safety and Health. Laura Ferreira Croft de Moura
2019 - 2020 Contribution in the supervision of a master student in Molecular Biology and Genetics. Vanessa Raquel Bento Guerra
2016 - 2016 Contribution in the supervision of a master student in Applied Microbiology. Ana João Azevedo de Oliveira
2015 - 2015 Contribution in the supervision of a master student in Veterinary Medicine. Catarina Lourenço da Cruz
2015 - 2015 Contribution in the supervision of a master student in Molecular Biology and Genetics. Ana Cristina Lopes dos Reis

Other jury / evaluation

Activity description Institution / Organization
2020/11 - Current Invited reviewer of an article, from BMC Microbiology Journal.
2020/02 - 2020/02 Invited reviewer of an article, from Applied Microbiology Journal.
Distinctions

Other distinction

2020 Accreditation by the National Board of Accreditation (IPAC) for Antimicrobial Susceptibility Testing by determination of Minimum Inhibitory Concentration assay (MIC) - NP 17025
2019 Qualification by the National Board of Accreditation (IPAC) for Salmonella differentiation of vaccine and field strains (NP 17025)
2010 PhD grant - SFRH/BD/62469/2009
2007 Merit Scholarship
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2006 Merit Scholarship
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
2005 Merit Scholarship
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal