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Biotechnology Researcher with expertise in metabolic engineering and a passion for synthetic biology. Specialized in manipulating yeast metabolism, particularly in the efficient production of fatty acids and metabolizing xylose. Extensive experience in academic research, complemented by several national and international collaborations with experts in the field. Demonstrated expertise in teaching, mentoring, and scientific writing. Effective communication skills and proactive engagement in outreach activities.
Identification

Personal identification

Full name
Paulo César Silva

Citation names

  • Silva, Paulo César

Author identifiers

Ciência ID
021F-4CDA-2630
ORCID iD
0000-0002-0931-8432

Websites

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Biochemistry
  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2024/02/26
Concluded
 Biologia (Doutoramento)
Major in Sem especialidade
Universidade do Minho, Portugal
"Metabolic engineering of sustainable branched chained unsaturateed fatty acid production in Saccharomyces Cerevisiae" (THESIS/DISSERTATION)
2018
Concluded
 Genética Molecular (Mestrado)
Major in Sem especialização
Universidade do Minho, Portugal
"The yeast DEL assay with dominant genetic markers" (THESIS/DISSERTATION)
2015
Concluded
 Biologia Aplicada (Licenciatura)
Universidade do Minho Escola de Ciências, Portugal
"n/a" (THESIS/DISSERTATION)
 17
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2024/09/01 - Current Invited Assistant Professor (University Teacher) Universidade do Minho, Portugal
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
Projects

Contract

Designation Funders
2018/10/01 - 2022 FatVal: Production of high value fatty acids and derived molecules from inexpensive carbon sources using recombinant robust industrial strain of Saccharomyces cerevisiae.
PTDC/EAM-AMB/32506/2017
PhD Student Fellow
Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Book chapter
  1. Humberto Pereira; Paulo César Silva; Björn Johansson. "Bacteria and Yeast Colony PCR". 2023.
    10.1007/978-1-0716-3358-8_17
Conference abstract
  1. Silva, Paulo César; Pereira, Humberto; Pozdniakova, Tatiana; Johansson, Björn. Corresponding author: Silva, Paulo César. "Bacterial branched-chain a-ketoacid dehydrogenase (BCKDH) expresses efficiently in Saccharomyces cerevisiae". Braga, 2021.
    Published
Conference paper
  1. Silva, Paulo César; Ceja-Navarro, Javier A.; Azevedo, Flávio Humberto Torres Dias Feio; Karaoz, Ulas; Brodie, Eoin L.; Johansson, Björn. Corresponding author: Silva, Paulo César. "A novel D-xylose isomerase: from the gut of a wood feeding beetle for improved conversion in Saccharomyces cerevisiae". Paper presented in Biocatalysis Open Day, 2020.
Conference poster
  1. Pozdniakova, Tatiana; Azevedo, Flávio; Pereira, Humberto; Silva, Paulo César; Johansson, Björn. "Fatty acid biosynthesis by Saccharomyces cerevisiae with a type II fatty acid synthase". Paper presented in 3rd BioIberoAmerica, 2022.
  2. Silva, Paulo César; Pereira, Humberto; Johansson, Björn. Corresponding author: Silva, Paulo César. "Expression of a branched-chain a-ketoacid dehydrogenase (BCKDH) from a non-conventional yeast in Saccharomyces cerevisiae". Paper presented in 15th International Conference on Yeasts, 2021.
  3. Silva, Paulo César; Ceja-Navarro, Javier A.; Azevedo, Flávio Humberto Torres Dias Feio; Karaoz, Ulas; Brodie, Eoin L.; Johansson, Björn. Corresponding author: Silva, Paulo César. "Functional screening for novel D-xylose isomerases from the gut of a wood feeding beetle reveals efficient expression in Saccharomyces cerevisiae". 2020.
  4. Silva, Paulo César; Ceja-Navarro, Javier; Azevedo, Flávio Humberto Torres Dias Feio; Karaoz, Ulas; Brodie, Eoin L.; Johansson, Björn. "A novel D-xylose isomerase from the gut of the wood feeding patent-leather beetle Odontotaenius disjunctus". Paper presented in 7th Conference on Physiology of Yeast and Filamentous Fungi, 2019.
  5. Pereira, Humberto; Silva, Paulo César; Azevedo, Flávio Humberto Torres Dias Feio; Johansson, Björn. "Functional expression of Yarrowia lipolytica acetyl- CoA carboxylase in Saccharomyces cerevisiae". Paper presented in 5th Applied Synthetic Biology in Europe, 2019.
Journal article
  1. Paulo César Silva; Björn Johansson. "Cell factories for sustainable production of valuable chemicals – the pivotal role of Saccharomyces cerevisiae". Journal UMinho Science (2024): http://dx.doi.org/10.21814/jus.5268.
    10.21814/jus.5268
  2. Tatiana A. Pozdniakova; João P. Cruz; Paulo César Silva; Flávio Azevedo; Pier Parpot; Maria Rosario Domingues; Magnus Carlquist; Björn Johansson. "Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae". Metabolic Engineering Communications (2023): http://dx.doi.org/10.1016/j.mec.2023.e00224.
    10.1016/j.mec.2023.e00224
  3. Nina Muratovska; Paulo Silva; Tatiana Pozdniakova; Humberto Pereira; Carl Grey; Björn Johansson; Magnus Carlquist. "Towards engineered yeast as production platform for capsaicinoids". Biotechnology Advances (2022): http://dx.doi.org/10.1016/j.biotechadv.2022.107989.
    10.1016/j.biotechadv.2022.107989
  4. Silva, Paulo César; Ceja-Navarro, Javier A.; Azevedo, Flávio; Karaoz, Ulas; Brodie, Eoin L.; Johansson, Björn. "A novel d-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae". Scientific Reports 11 1 (2021): http://dx.doi.org/10.1038/s41598-021-83937-z.
    Published • 10.1038/s41598-021-83937-z
  5. Silva, Paulo César; Domingues, Lucília; Collins, Tony; Oliveira, Rui; Johansson, Björn. "Quantitative assessment of DNA damage in the industrial ethanol production strain Saccharomyces cerevisiae PE-2". FEMS Yeast Research 18 8 (2018): http://dx.doi.org/10.1093/femsyr/foy101.
    10.1093/femsyr/foy101
Preprint
  1. Humberto Pereira; Paulo César Silva; Mikel Davis; Louis Abraham; Gyorgy Babnigg; Henrik Bengtsson; Björn Johansson. "SEGUID v2: Extending SEGUID checksums for circular, linear, single- and double-stranded biological sequences". 2024. http://dx.doi.org/10.1101/2024.02.28.582384.
    10.1101/2024.02.28.582384
Thesis / Dissertation
  1. Silva, Paulo César. "The Yeast DEL Assay With Dominant Genetic Markers". Master, Universidade do Minho Escola de Ciências, 2018. http://repositorium.sdum.uminho.pt/handle/1822/55873.

Intellectual property

Patent
  1. Silva, Paulo César; Ceja-Navarro, Javier A.; Brodie, Eoin L.; Johansson, Björn. 2020. "A novel xylose isomerase gene and polypeptide and uses".
    Pending
  2. Ceja-Navarro, Javier A.; Silva, Paulo César; Johansson, Björn; Brodie, Eoin L.. 2020. "A novel xylose isomerase gene and polypeptide and uses thereof". United States.
    Pending
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2020/11/26 A novel D-xylose isomerase: from the gut of a wood feeding beetle for improved conversion in Saccharomyces cerevisiae Biocatalysis Open Day
European Federation of Biotechnology

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/09/01 - 2022/10/30 Metabolic engineering for the production of branched-chain fatty acids from D-xylose in Saccharomyces cerevisiae
Co-supervisor of Francisco Magalhães
 Genética Molecular (Master)
Universidade do Minho, Portugal
2020/04/01 - 2020/07/31 Development of a sugar quantification method in cultures of Saccharomyces cerevisiae
Co-supervisor of Francisco Magalhães
 Bioquímica (Degree)
Universidade do Minho, Portugal
2020/04/01 - 2020/07/31 Yeast DEL assay with a fluorescent genetic marker
Co-supervisor of Francisca Rodrigues
 Biologia Aplicada (Degree)
Universidade do Minho, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2014/09/01 - 2015/06/30 Applied Biology Days XVI - a student-organized conference featuring 8 plenary talks, 10 workshops, and a science talk show. (2015/02/17 - 2015/02/18)
Conference (President of the Organising Committee)
 Universidade do Minho, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2019 - Current Lectures at high schools regarding my field of research and experience as a scientist.
Seminar
 Researchers go back to school
Universidade do Minho, Portugal
2023/05 - 2023/05 Informal science communication entitled "Cell factories for a sustainable society"
Festival
 Pint of Science
2022/09 - 2022/09 Coordination of the hands-on activity "Yeast biotechnology - diversity and applications".
Exhibition
 European Researchers’ Night
Universidade do Minho, Portugal
2021/11/17 - 2021/11/18 Joint FEBS/SPB/UMinho Symposium on Evidence Based Teaching in Your Biomolecular Classroom: Catalysing Student Engagement
Workshop
Universidade do Minho Centro IDEA, Portugal
2021/10/19 - 2021/10/22 Computational Approaches to Understanding and Engineering Enzyme Catalysis
Workshop
Federation of European Biochemical Societies, United Kingdom
2021/09 - 2021/09 Coordination of the hands-on activity "Microbial cell factories towards a sustainable future".
Exhibition
Universidade do Minho, Portugal
2019/09 - 2019/09 Coordination of the hands-on activity "Sustainable cities and 3rd generation biofuels".
Exhibition
 European Researchers’ Night
Universidade do Minho, Portugal
2018/09 - 2018/09 Coordination of the hands-on activity "Yeast: a biotechnological factory".
Exhibition
 European Researchers’ Night
Universidade do Minho, Portugal
2018/04 - 2018/04 Workshop on “Syllabus and course design”
Workshop
University of California Berkeley, United States
2018/03 - 2018/03 Fulbright Enrichment Seminar – “Building an Innovation Ecosystem in St. Louis”
Seminar
Fulbright Association, United States

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2021/03/05 Discovery of a new Xylose Isomerase with the potential for producing biofuels and pharmaceuticals. Correio do Minho
2021/03/04 Industrial potential of the newly discovered Xylose Isomerase. RUM - Rádio Universitária do Minho

Mentoring / Tutoring

Topic Student name
2022/09/01 - 2023/10/31 Expression of a discrete, mono-functional FAS type II in industrial Saccharomyces cerevisiae strains Júlio Freire
2021/04/01 - 2021/07/31 Eco-efficiency in the Molecular Genetics Lab: optimization of an agarose gel electrophoresis buffer Tomás Neves
Distinctions

Award

2022 FEBS Short-term Fellowship
Federation of European Biochemical Societies, United Kingdom
2018 FCT PhD Studentship
Fundação para a Ciência e a Tecnologia, Portugal
2017 Fulbright Grant Award
Fulbright Portugal, Portugal
2015 Excellence Scholarship
Universidade do Minho, Portugal