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After graduating with a degree in Environmental Engineering in 2010, I immediately applied and completed my master's degree in the same field at the Agricultural School of the Polytechnic Institute of Beja. Although my degree has given me a strong background in the field of environmental quality assessment, my master’s degree in Environmental Engineering also allowed me to deepen my knowledge in this area, particularly during my master's trainee, at CEBAL, where I approached the Valorization of lignocellulosic biomass: Studies of enzymatic saccharification of Cardoon and Rockrose. Since then, I have worked as a researcher fellow on the Bioactive Compounds Research Group at CEBAL, under the supervision of Dr. Maria de Fátima Duarte, where I was awarded two fellowships within the projects: ValBioTecCynara - Economic valorization of Cardoon (Cynara cardunculus): study of natural variability and biotechnological applications (ALT20-03-0145-FEDER-000038 ALENTEJO 2020) and MedCynaraBioTec - Selection of Cynara cardunculus Genotypes for New Biotechnological Applications: the value chain improvement of cardoon, a well-adapted Mediterranean crop (ALT20-03-0145-FEDER-039495). During the last few years, I have been acquiring experience in a wide range of chemical and biochemical procedures through the characterization of the biological potential of extracts from different biomasses, and chemical characterization/quantification, but also in cell and molecular biology techniques. Since 2020, I have been a PhD student in the BEAG doctoral program at the University of Lisbon. The research focuses on molecular markers. The project intends to study the development of molecular markers to select the best cardoon genotypes for cynaropicrin production, leading to the basis of a future breeding program. With great biological activity, Cynaropicrin can be valorized within different industries, being the pharmaceutical and biotechnological options being the ones with greater added value. Chemical Cynara carcunculus profile, genetic variability, transcriptomic studies, and identification of potential molecular markers associated with cynaropicrin traits will be assessed.
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Identification

Personal identification

Full name
Ana Filipa Costa Paulino

Citation names

  • Paulino, Ana
  • Paulino, Ana Filipa

Author identifiers

Ciência ID
DA13-B482-D8ED
ORCID iD
0000-0003-0742-9005

Email addresses

  • ana.paulino@cebal.pt (Professional)

Telephones

Telephone
  • 284314399 (Professional)

Addresses

  • Rua Pedro Soares, Apartado 6158, 7801-908, Beja, Beja, Portugal (Professional)

Knowledge fields

  • Agrarian Sciences - Agriculture, Forestry and Fisheries - Plant Breeding and Plant Protection
  • Natural sciences - Biological Sciences
  • Agrarian Sciences - Agrarian and Food Biotechnology - Marker Assisted Selection

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
English Elementary (A2) Intermediate (B1) Elementary (A2) Intermediate (B1)
Education
Degree Classification
2020/01/01 - 2023/12/31
Ongoing
 Biologia e Ecologia das Alterações Globais (Doutoramento)
Major in Biologia do Genoma e Evolução
Universidade de Lisboa Faculdade de Ciências, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal

Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2021/03/01 - 2021/03/05
Concluded
 Strategies for Citizen Engagement in Science Communication (Outros)
Universidade de Lisboa Faculdade de Ciências, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal
2021/01/18 - 2021/01/23
Concluded
 Hands on Functional Diversity: from Ecological Indicators to Ecosystem Services (Outros)
Universidade de Lisboa Faculdade de Ciências, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal
2021/01/06 - 2021/01/08
Concluded
 Reproducibility in Science (Outros)
Universidade de Lisboa Faculdade de Ciências, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal
2020/09/07 - 2020/09/11
Concluded
 Science and the Media – bringing together scientists, journalists and society (Outros)
Universidade de Lisboa Faculdade de Ciências, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal
2020/06/15 - 2020/06/18
Concluded
 Advanced R for Ecology and Evolutionary Biology (Outros)
Universidade de Lisboa Faculdade de Ciências, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal
2020/06/03 - 2020/06/05
Concluded
 Introduction to R for Ecology and Evolutionary Biology (Outros)
Universidade de Lisboa Faculdade de Ciências, Portugal

Centro de Ecologia Evolução e Alterações Ambientais, Portugal
2011/10 - 2013/09/27
Concluded
 Mestrado em Engenharia do Ambiente (Mestrado)
Instituto Politécnico de Beja Escola Superior Agrária, Portugal
"Valorization of lignocellulosic biomass: Studies of enzymatic saccharification of Cardoon and Rock rose" (THESIS/DISSERTATION)
 17
2007 - 2010/10/07
Concluded
 Engenharia do Ambiente (Licenciatura)
Instituto Politécnico de Beja Escola Superior Agrária, Portugal
"Gestão de Resíduos de Construção e Demolição no Município de Beja" (THESIS/DISSERTATION)
 13
Affiliation

Others

Category
Host institution 		Employer
2020/01/01 - 2024/12/25 Estudante de Doutoramento Associação para a Inovação e Desenvolvimento da FCT, Portugal
Universidade de Lisboa Faculdade de Ciências, Portugal
(...)
2018/10/01 - 2019/08/31 Scientific Research - Inov@sfileiras, PDR2020, tem como âmbito de intervenção a observação de um conjunto de fileiras emergentes e inovadoras Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2016/09/01 - 2018/09/30 Scientific Research Fellow - ValBioTecCynara - economic valorization of Cardoon: study of natural variability and biotechnological applications. ALT20-03- 0145-FEDER- 000038 ALENTEJO 2020. Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2015/11/01 - 2015/12/31 Scientific Research Fellow - In vitro culture of animal cells (breast); Antiproliferative activity; Metabolomics Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2015/07/06 - 2015/09/30 Scientific Research Fellow - “NeucBark - New Valorization Strategies for Eucalyptus spp. Bark Extracts” financiado pela FCT (Refª. PTDC/AGR-FOR/3187/2012) In vitro culture of animal cells (breast); An Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2015/04/22 - 2015/06/04 Employment of Insertion - In vitro culture of animal cells (breast); Anti-proliferative activity; Metabolomics and Western Blot. Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2014/01/02 - 2015/01/01 trainee recruitment - Research on the utilization potential of opuntia ficus indica pulp production's resultant waste and its seeds oil production. Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Projects

Grant

Designation Funders
2020/01/01 - 2024/12/25 Molecular Markers associated to the cynaropicrin trait in Portuguese cardoon population
SFRH/BD/145383/2019
10.54499/SFRH/BD/145383/2019
Universidade de Lisboa Faculdade de Ciências, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/09/01 - 2018/09/30 ValBioTecCynara - economic valorization of Cardoon (Cynara cardunculus): study of natural variability and biotechnological applications
ALT20-03- 0145-FEDER- 000038
Research Fellow
Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Concluded

Contract

Designation Funders
2019 - Current Inov@sFileiras
PDR2020-2024-032732
Researcher
European Agricultural Fund for Rural Development
2019/04/01 - 2022/03/31 MedCynaraBioTec - Seleção de Genótipos de Cynara cardunculus Para Novas Aplicações Biotecnológicas: potenciar a cadeia de valor do cardo, uma cultura mediterrânica bem adaptada
SAICT-ALT/39495/2018
Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal

Instituto Politécnico de Beja, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Cristina Conceição; Pedro Martins; Nuno Alvarenga; João Dias; Elsa Lamy; Lúcia Garrido; Sandra Gomes; et al. "Cynara cardunculus: Use in Cheesemaking and Pharmaceutical Applications". In Technological Approaches for Novel Applications in Dairy Processing, 73-107. intechopen, 2018.
    Published
Conference abstract
  1. Paulino, Ana; Castro, MM; Rosa, Daniela; Ferro, AM; Faustino, Ana; Bras, Teresa; Machado, E; et al. "Molecular studies in genetic characterization of cardoon germplasm". Paper presented in II Simpósio Nacional de Valorização do Cardo, Beja, 2023.
  2. Paulino, Ana; Fernandes, I; Pires, Rita; Faustino, Ana; Santos, J; Bras, Teresa; Rosa, Daniela; et al. "Differential expression analysis of genes involved in cynaropicrin production in Cynara cardunculus". Paper presented in II Simpósio Nacional de Valorização do Cardo, Beja, 2023.
  3. 2818-622D-0190; DA13-B482-D8ED; E917-85D6-212E; D11F-2A18-B1D3; DE1F-F67B-B2B7. "Cynara cardunculus sesquiterpene lactone cynaropicrin variation– from earlier stages till flowering". Paper presented in XI International Symposium on artichoke, cardoon and their wild relatives, Molfetta, 2023.
  4. DA13-B482-D8ED; Pires, Rita Costa; Isabel Fernandes; 8F1B-34B2-BB60; E917-85D6-212E; 2818-622D-0190; 011A-339A-350C; et al. "Comparative RNA-Seq analysis of distinct cynaropicrin productions on different Cynara cardunculus L. populations". Paper presented in XI International Symposium on artichoke, cardoon and their wild relatives, Molfetta, 2023.
  5. Santos, JO; Paulino, Ana; Bras, Teresa; Rosa, Daniela; Maria F. Duarte. "Relationship between inflorescence properties and seed production in Cynara cardunculus L.". Paper presented in III Colóquio Nacional de PlantasAromáticas e Medicinais (CNPAM2022), Castelo Branco, 2022.
    Accepted
  6. Rosa, Daniela; Bras, Teresa; Paulino, Ana; Macias, Francisco A; Maçãs, Benvindo; Maria F. Duarte. "Cynara cardunculus leaves extract fractioning and phytotoxic evaluation". Paper presented in VI Encontro de Estudantes de Doutoramento em Ambiente e Agricultura, Évora, 2021.
    Published
  7. Paulino, Ana; Santos, Jacqueline; Pereira, Margarida; Bras, Teresa; Rosa, Daniela; Paulo, Octávio Salgueiro; Marum, Liliana; Fátima Duarte. "Morphological characterization of Cynara cardunculus L. (cardoon) in southern Portugal". Paper presented in VI PhD Students Meeting in Environmental and Agriculture, Évora, 2021.
    Published
  8. Rosa, Daniela; Bras, Teresa; Paulino, Ana; Macías, Francisco A; Maçãs, Benvindo; Duarte, MF. "Cynara cardunculus leaves extract fractionation - an enriched source of allelochemicals". Paper presented in V Encontro de Estudantes de Doutoramento em Ambiente e Agricultura, Évora, 2020.
    Published
  9. Bras, Teresa; Paulino, Ana; Rosa, Daniela; Gomes, AC; Neves, LA; Crespo, J; Maria F. Duarte. "Cynaropicrin from Cynara cardunculus anti-inflammatory potential: chitosan wound dressing application". Paper presented in Phytochemical Society of Europe eCongress 2020, 2020.
  10. Paulino, Ana; Bras, Teresa; Rosa, Daniela; Belo, AF; Carla Pinto-Cruz; Guerreiro, I; Regato, M; et al. "Molecular Markers associated to the cynaropicrin trait in portuguese cardoon population". Paper presented in IV Encontro de Estudantes de Doutoramento em Ambiente e Agricultura, Évora, 2019.
    Published
  11. Castro, MM; Guerra, A; Paulino, Ana; Bras, Teresa; Portugal, J; Ramõa, S; Nozes, P; et al. "Bioactivity of Cynara cardunculus leaves extracts, as a source of cynaropicrin, in a breast cancer triple negative cell model". Paper presented in 3º International PSE Symposium on Natural Products in Cancer Prevention and therapy, Nápoles, 2018.
    Published
  12. Paulino, Ana; Bras, Teresa; Machado, E; Rosa, Daniela; Castro, MM; Ferro, AM; Marum, Liliana; et al. "Cynara cardunculus experimental field installation: a source of natural variability". Paper presented in 7th International Conference on Engineering for Waste and Biomass Valorization, Praga, 2018.
    Published
  13. Bras, Teresa; Paulino, Ana; Neves, LA; Crespo, JG; Duarte, MF. "Fractionation of ethanolic ultrasound exctracts from Cynara cardunculus by membrane separation processes". Paper presented in 7th International Conference on Engineering for Waste and Biomass Valorisation, Praga, 2018.
    Published
  14. Paulino, Ana; Bras, Teresa; Rosa, Daniela; Castro, MM; Almeida, E; Portugal, J; Ramõa, S; Nozes, P; Duarte, MF. "Natural variability of Cynara cardunculus leaves biomass – a source of cynaropicrin". Paper presented in 14th International Conference on Renewable Resources and Biorefineries, Ghent, 2018.
    Published
Conference paper
  1. Ana Paulino; Teresa Brás; Daniela Rosa; Rita Costa Pires; Jacqueline Santos; Margarida Pereira; Octávio S. Paulo; Liliana Marum; Mª Fátima Duarte. "Chemical and Genetic Relationships of Cynara cardunculus L. (Cardoon) in Southern Portugal". 2021.
    10.3390/IECPS2021-12011
Conference poster
  1. Paulino, Ana Filipa; Fernandes, Isabel; Usié, Ana; Pires, Rita Costa; Santos, Jacqueline; Faustino, Ana; Bras, Teresa; et al. "The molecular regulatory mechanisms behind the vegetative-to-reproductive transition in Cynara cardunculus". Paper presented in Bioinformatics Open Days 2025 - XIV Edition, 2025.
  2. Paulino, Ana; Rodrigues, Cristiano; Faustino, Ana; Peixe, Augusto; Paulo, Octávio Salgueiro; Maria F. Duarte; Marum, Liliana. "In Vitro Propagation, Acclimatization, and transplantation into the field of Cynara cardunculus L. ecotypes". Paper presented in IX PhD Students Meeting in Environment and Agriculture, 2024.
  3. Paulino, Ana; Fernandes, I; Usié, Ana; Pires, Rita; Santos, J; Faustino, Ana; Bras, Teresa; et al. "RNA-Seq analysis at two different developmental stages of Cynara cardunculus". Paper presented in VIII PhD Students Meeting in Environment and Agriculture, 2023.
  4. Faustino, Ana; Paulino, Ana; Pires, Rita; Marinho, Cláudia; Ferro, Ana; Capote, T.; Usié, A.; et al. Corresponding author: Marum, L.. "Advances in genetic and propagation tools to assist the climate change challenges in the Mediterranean region". Paper presented in Science CHANGing Policy, 2023.
  5. DA13-B482-D8ED; Pires, Rita Costa; 0F13-E4FD-F6AF; 8F1B-34B2-BB60; E917-85D6-212E; 2818-622D-0190; 011A-339A-350C; et al. "Genome-wide transcriptomic analysis reveals novel genes involved in cynaropicrin synthesis in Cynara cardunculus". Paper presented in XII Edition Bioinformatics Open Days, 2023.
  6. Paulino, Ana; Pires, Rita; Fernandes, I; Santos, J; Bras, Teresa; Rosa, Daniela; Paulo, Otávio S.; Duarte, MF; Marum, Liliana. "RNA-Seq reveals differential expression profiles and functional annotation of genes involved in cynaropicrin synthesis in cardoon". Paper presented in VII PhD Students Meeting in Environment and Agriculture, 2022.
  7. Santos, Jacqueline; Pereira, A. M.; Paulino, Ana; Bras, Teresa; Rosa, Daniela; Fátima Duarte. "The interplay of cynaropicrin concentration and leaves development in Cynara cardunculus L.". Paper presented in Colóquio Nacional de Plantas Aromáticas e Medicinais, 2022.
  8. Rosa, Daniela; Bras, Teresa; Paulino, Ana; Macias, Francisco A; Maçãs, Benvindo; Duarte, MF. "Bioherbicide Cynara: weed control through natural resources for sustainable agriculture". Paper presented in Natural Products Application: Health, Cosmetics and Food, 2021.
  9. Paulino, Ana; Santos, Jacqueline; Bras, Teresa; Rosa, Daniela; Pires, Rita; Belo, ADF; Carla Pinto-Cruz; et al. "Cynara cardunculus leaves - a natural source of cynaropicrin". Paper presented in V PhD Students Meeting in Environmental and Agriculture, 2020.
  10. Paulino, Ana; Bras, Teresa; Rosa, Daniela; Pires, Rita; Paulo, O.S.; Marum, Liliana; Duarte, M.F.. "Genetic and Chemical relationship of Cynara cardunculus populations - a natural source of cynaropicrin". Paper presented in Ciência 2020 - Encontro com a Ciência e Tecnologia em Portugal, 2020.
  11. Paulino, Ana; Bras, Teresa; Rosa, Daniela; Gomes, S.; Martins, A.P.L.; Duarte, M.F.. "Evaluation of natural variability of Cynara cardunculus – a comparative study". Paper presented in PSE E-Congress 2020, Plant derived natural products as pharmacological and nutraceutical tools, 2020.
  12. Paulino, Ana; Bras, Teresa; Rosa, Daniela; Pires, Rita; Paulo, O.S.; Marum, Liliana; Duarte, M.F.. "Chemical and genetic relationship of Cynara cardunculus populations-a natural source of cynaropicrin". Paper presented in PSE E-Congress 2020, Plant derived natural products as pharmacological and nutraceutical tools, 2020.
  13. Bras, Teresa; Paulino, Ana; Rosa, Daniela; Neves, L.A.; Crespo, J.P.G.; Duarte, M.F.. "Cynara Cardunculus as a source of cynaropicrin – extraction and purification". Paper presented in IV PhD Students Meeting in Environmental and Agriculture, 2019.
  14. Rosa, Daniela; Bras, Teresa; Paulino, Ana; Macias, Francisco A; Maçãs, Benvindo; Maria F. Duarte. "Bioherbicide Cynara: weed control trough natural resources for sustainable agriculture". Paper presented in IV Encontro de Estudantes de Doutoramento em Ambiente e Agricultura, 2019.
  15. Bras, Teresa; Paulino, Ana; Rosa, Daniela; Neves, LA; Crespo, J; Maria F. Duarte. "Extraction and Purification of Cynaropicrin from Cynara Cardunculus leaves". Paper presented in PSE Meeting - Natural Products in Drug Discovery and Human Health, 2019.
  16. Castro, Maria Miguel ; Rosa, Daniela; Paulino, Ana; Ferro, A.M.; Bras, Teresa; Mendonça, D.; Simões, F.; et al. "Genetic, Morphological and Biochemical Characterization of Cynara cardunculus from Alentejo Region". Paper presented in Jornadas MED, 2019.
  17. Marum, Liliana; Bras, Teresa; Castro, Maria Miguel ; Paulino, Ana; Carla Pinto-Cruz; Belo, ADF; Regato, Mariana; et al. "Medcynarabiotec: a strategy towards selection of Cynara cardunculus genotypes for new biotechnological applications". Paper presented in 5º Simpósio: Produção e Transformação de Alimentos em Ambiente Sustentável, 2019.
  18. Castro, Maria Miguel ; Guerra, Angela (DB1D-6B63-5C2E); Paulino, Ana; Bras, Teresa; Portugal, J.; Ramôa, S.; Nozes, P.; et al. "Bioactivity of Cynara cardunculus leaves extracts, as a source of cynaropicrin, in a breast cancer triple negative cell model". Paper presented in 3º International PSE Symposium on Natural Products in Cancer Prevention and therapy, 2018.
  19. Paulino, Ana; Bras, Teresa; Machado, E.; Rosa, Daniela; Castro, Maria Miguel ; Ferro, A.M.; Marum, Liliana; et al. "Cynara cardunculus experimental field installation: a source of natural variability". Paper presented in 7th International Conference on Engineering for Waste and Biomass Valorisation, 2018.
  20. Guerra, Angela (DB1D-6B63-5C2E); Calçada, C.; Soares, B.I.G.; Paulino, Ana; Oliveira, H.; Freire, C.S.R.; Silvestre, A.J.D.; et al. "Antiproliferative effects of a lipophilic Eucalyptus bark extract on breast cancer cells". Paper presented in 14th International Conference on Renewable Resources and Biorefineries, 2018.
  21. Gomes, S.; Trigo, M.J; Belo, A.T.; Alvarenga, N.B.; Dias, J.; Lage, P.; Carvalho, MJ; et al. "Characterization of Cynara cardunculus L. flower from Alentejo as coagulant agent for cheesemaking". Paper presented in 10th Cheese Symposium, 2018.
  22. Castro, Maria Miguel ; Ramos, P. A. B.; Bras, Teresa; Paulino, Ana; Guerra, Angela (DB1D-6B63-5C2E); Oliveira, H.; Freire, C.S.R.; Silvestre, A.J.D.; Duarte, M.F.. "Bioactivity of Cynara cardunculus leaves lipohilic extracts within a breast cancer triple negative cell model". Paper presented in Trends in Natural Product Research – PSE Young Scientists´Meeting, Natural Products in Health, Agro-Food and Cosmetic, 2017.
  23. Ferro, A.M.; Machado, M.M.; Bras, Teresa; Paulino, Ana; Belo, A.F.; Simões, M.P.; Carla Pinto-Cruz; et al. "Genetic, Morphological and Biochemical Characterization of a Cynara cardunculus Collection from Alentejo". Paper presented in XLI Jornadas Portuguesas de Genética, 2017.
  24. Guerra, Angela (DB1D-6B63-5C2E); Domingues, R.; Paulino, Ana; Guerreiro, Olinda; Oliveira, H.; Freire, C.; Neto, C.; et al. "Anti-tumoral activity of triterpenic-enriched Eucalyptus spp. bark extracts". Paper presented in Ciência 2016 - Encontro com a Ciência e Tecnologia em Portugal, 2016.
  25. Paulino, Ana; Ferro, M.; Chaves, H.T.; Evtuguin, D.V.; Xavier, A.M.R.B.; Bohn, A.; Fernandes, M.C.. "Optimization of Enzymatic Hydrolysis of Dilute-Acid Pretreated Cardoon". Paper presented in Microbiotec´13- Portuguese Congress of Microbiology and Biotechnology, 2013.
  26. Paulino, Ana; Ferro, M.; Fernandes, M.C.; Prozil, S.O.; Mendes, J.A.S.; Chaves, H.T.; Gravitis, Janis; Xavier, A.M.R.B.; Evtuguin, D.V.. "Reaction vessel effect on the enzymatic hydrolysis of pretreated Cistus ladanifer and Cynara cardunculus". Paper presented in 1º Congresso Internacional de Recursos Silvestres, 2013.
  27. Xavier, A.M.R.B.; Fernandes, Maria C; Paulino, Ana; Prozil, S.O.; Mendes, J.A.S.; Bonin, T.; Chaves, H.T.; Gravitis, Janis; Evtuguin, D.V.. "Effect of Dilute Acid Hydrolysis and Steam Explosion Pretreatments on the Enzymatic Saccharification of Cistus ladanifer and Cynara cardunculus". Paper presented in 9th RRB – 9th International Conference on Renewable Resources & Biorefineries, 2013.
  28. Fernandes, M.C.; Paulino, Ana; Gravitis, Janis; Chaves, H.T.; Evtuguin, D.V.; Xavier, A.M.R.B.. "Effect of Dilute Acid Hydrolysis and Steam Explosion Pretreatments on the Enzymatic Saccharification of Cistus ladanifer and Cynara cardunculus". Paper presented in X Jornadas CICECO, 2013.
  29. Paulino, Ana; Ferro, Miguel; Fernandes, Maria C; Prozil, S.O.; Mendes, J.A.S.; Chaves, H.T.; Gravitis, Janis; Xavier, A.M.R.B.; Evtuguin, D.V.. "Reaction vessel effect on the enzymatic hydrolysis of pretreated Cistus ladanifer and Cynara cardunculus". Paper presented in II Congreso Iberoamericano sobre Biorrefinerías (2-CIAB), 2013.
Journal article
  1. Paulino, A.; Pires, R.C.; Fernandes, I.; Faustino, A.; Santos, J.; Usié, A.; Brás, T.; et al. "Comparative RNA-Seq analysis of Cynara cardunculus L. at different developmental stages". Acta Horticulturae 1424 (2025): 145-154. https://doi.org/10.17660/actahortic.2025.1424.18.
    Published • 10.17660/actahortic.2025.1424.18
  2. Paulino, A.; Pires, R.C.; Fernandes, I.; Santos, J.; Brás, T.; Rosa, D.; Paulo, O.S.; Duarte, M.F.; Marum, L.. "Transcriptome analysis of Cynara cardunculus in southern Portugal". Acta Horticulturae 1362 (2023): 351-358. http://dx.doi.org/10.17660/actahortic.2023.1362.47.
    10.17660/actahortic.2023.1362.47
  3. Castro, Maria Miguel; Rosa, Daniela; Ferro, Ana M.; Faustino, Ana; Paulino, Ana; Brás, Teresa; Machado, Eliana; et al. "Genetic diversity and population structure of Cynara cardunculus L. in southern Portugal". PLOS ONE 16 6 (2021): e0252792. http://dx.doi.org/10.1371/journal.pone.0252792.
    10.1371/journal.pone.0252792
  4. Bras, Teresa; Paulino, Ana; Neves, Luísa A.; Crespo, João G.; Duarte, Maria F.. "Ultrasound assisted extraction of cynaropicrin from Cynara cardunculus leaves: Optimization using the response surface methodology and the effect of pulse mode". Industrial Crops and Products 150 (2020): 112395. http://dx.doi.org/10.1016/j.indcrop.2020.112395.
    Published • 10.1016/j.indcrop.2020.112395
  5. Dias, João; Lage, Patricia; Garrido, Ana; Machado, Eliana; Conceição, Cristina; Gomes, Sandra; Martins, António; et al. "Evaluation of gas holes in Queijo de Nisa PDO cheese using computer vision". Journal of Food Science and Technology 58 3 (2020): 1072-1080. http://dx.doi.org/10.1007/s13197-020-04621-0.
    Published • 10.1007/s13197-020-04621-0
  6. Fernandes, M.C.; Ferro, M.D.; Paulino, A.F.C.; Chaves, H.T.; Evtuguin, D.V.; Xavier, A.M.R.B.. "Comparative study on hydrolysis and bioethanol production from cardoon and rockrose pretreated by dilute acid hydrolysis". Industrial Crops and Products 111 (2018): 633-641. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034829548&partnerID=MN8TOARS.
    10.1016/j.indcrop.2017.11.037
  7. Râmoa, Sofia; Portugal, João; Travlos, Ilias; Vasconcelos, Teresa; Forte, Paulo; Oliveira e Silva, Pedro; Nozes, Paula; et al. "Evaluación de los cambios en la flora espontánea en Portugal para un periodo de 10 años. Proyecto ValBioTecCynara". (2017): http://hdl.handle.net/10174/22532.
  8. Fernandes, M.C.; Ferro, M.D.; Paulino, A.F.C.; Mendes, J.A.S.; Gravitis, J.; Evtuguin, D.V.; Xavier, A.M.R.B.. "Enzymatic saccharification and bioethanol production from Cynara cardunculus pretreated by steam explosion". Bioresource Technology 186 (2015): 309-315. http://www.scopus.com/inward/record.url?eid=2-s2.0-84943589397&partnerID=MN8TOARS.
    10.1016/j.biortech.2015.03.037
  9. Ferro, M.D.; Fernandes, M.C.; Paulino, A.F.C.; Prozil, S.O.; Gravitis, J.; Evtuguin, D.V.; Xavier, A.M.R.B.. "Bioethanol production from steam explosion pretreated and alkali extracted Cistus ladanifer (rockrose)". Biochemical Engineering Journal 104 (2015): 98-105. http://www.scopus.com/inward/record.url?eid=2-s2.0-84947866077&partnerID=MN8TOARS.
    10.1016/j.bej.2015.04.009
Magazine article
  1. Bras, Teresa; Paulino, Ana; Rosa, Daniela; Santos, Jacqueline; Marum, Liliana; Paulo, Otávio S.; Neves, Luísa; et al. "A folha do cardo como fonte de compostos bioativos de valor económico", A Voz do Campo, 2020, https://vozdocampo.pt/2020/11/18/a-folha-do-cardo-como-fonte-de-compostos-bioativos-de-valor-economico/.
  2. 2818-622D-0190; Paulino, Ana; Santos, Jacqueline. "A folha do cardo como fonte de compostos bioativos", Revista voz do campo, 2020, https://vozdocampo.pt/.
  3. Parreira, Paula; Brás, Teresa; Paulino, Ana; Ferro, Ana; Duarte, MF. "Cardo- Um recurso endógeno de elevado valor acrescentado", Voz do Campo , 2016
Thesis / Dissertation
  1. Paulino, Ana. "Valorização da biomassa lenhocelulósica: estudo de sacarificação enzimática do cardo e da esteva". Master, 2013. http://hdl.handle.net/20.500.12207/632.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/04/18 Comparative RNA-Seq analysis of distinct cynaropicrin productions on different Cynara cardunculus L. populations XI International Symposium on artichoke, cardoon and their wild relatives
(Molfetta, Italy)
2023/04/18 Cynara cardunculus sesquiterpene lactone cynaropicrin variation– from earlier stages till flowering XI International Symposium on artichoke, cardoon and their wild relatives
(Molfetta, Italy)
2023/03 Differential expression analysis of genes involved in cynaropicrin production in Cynara cardunculus II Simpósio Nacional de Valorização do Cardo
Centro de Biotecnologia Agrícola e Agroalimentar do Alentejo (Beja, Portugal)
2021/12/09 Morphological characterization of Cynara cardunculus L. (cardoon) in southern Portugal VI PhD Students Meeting in Environmental and Agriculture
Universidade de Évora (Évora, Portugal)
2019 Molecular Markers associated to the cynaropicrin trait in portuguese cardoon population IV Encontro de Estudantes de Doutoramento em Ambiente e Agricultura
Universidade de Évora (Évora, Portugal)
2018/12/05 Cynara cardunculus experimental field: a source of natural variability Painel III - A Planta do Cardo: da Sua Variabilidade Genética e Morfológica ao seu Potencial Bioquímico I Simpósio Nacional de Valorização do Cardo - Seminário de Encerramento do Projeto ValBioTecCynara
CEBAL - Instituto Politécnico de Beja (Beja, Portugal)
2018/07/02 Fractionation of ethanolic ultrasound exctracts from Cynara cardunculus by membrane separation processes 7th International Conference on Engineering for Waste and Biomass Valorisation
(Praga, Czech Republic)
2018/05/31 Natural variability of Cynara cardunculus leaves biomass – a source of cynaropicrin 14th International Conference on Renewable Resources and Biorefineries
(Ghent, Belgium)
2018/02/05 Cynaropicrin antiproliferative effect on a breast cancer cell model XLVIII Reunião Anual da Sociedade Portguesa de Farmacologia. XXXVI Reunião de Farmacologia Clínica e XVII Reunião de Toxicologia. Session 8 - Cancer Pharmacology II
Faculdade de Medicina da Universidade de Lisboa (Lisboa, Portugal)
2017/10/25 Evaluación de los cambios en la flora espontánea en Portugal para un periodo de 10 años. Proyecto ValBioTecCynara XVI Congreso de la Sociedad Española de Malherbología
Universidad Pública de Navarra (Pamplona-Iruña, Spain)
2017/10/01 Effect of pulsed ultrasound assisted extraction upon cynaropicrin concentration: extraction optimization using response surfasse methodology 10th World Congress Of Chemical Engineering
(Barcelona, Spain)
2017/06/02 Cynara Pasta – Uma abordagem integrada para a valorização da folha de Cynara cardunculus. 3º Simpósio Produção e Transformação de Alimentos: Sustentabilidade, Inovação e Nutrição – Sessão Plenária – Inovação Alimentar e Nutrição
Instituto politécnico de Leiria (Leiria, Portugal)
2016/11/09 Investigação na Valorização da Biomassa Transferência de Tecnologias em Ambiente - 1as Jornadas Técnicas da Escola Superior Agrária
Instituto Politécnico de Beja, Escola Superior Agrária (Beja, Portugal)
2015/06/03 Steam explosion pretreatment of Cynara cardunculus for bioethanol production 11th RRB – 11th International Conference on Renewable Resources & Biorefineries
University of York (United Kingdom)
2015/05/27 “Produção de bioetanol de segunda geração” 0º Seminário de Engenharia das Energias Renováveis e Ambiente
Escola Superior de Tecnologia e Gestão de Portalegre, Instituto Politécnico de Portalegre (Portalegre)
2014/09/10 Bioethanol production from rockrose pretreated by steam explosion 12nd international chemical and biological engineering conference
(Porto, Portugal)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2023/05/30 - 2023/05/31 The Applied Agro-BiotechnologyInternational Meeting (AABIM) is a novel scientific event that aims to approach the most up-to-date scientific developments in the related fields of agro-biotechnology. Being held in Beja (Alentejo, PT), the thematic of this first edition will address the New Approaches for a Qualitative Valorization of the Olive Oil Sector, since the olive oil sector is one of the most relevant and continuously growing industries both in Alentejo and the whole Mediterranean region. (2023/05/30 - 2023/05/31)
Congress (Member of the Organising Committee)
 Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
2023/03/23 - 2023/03/23 II Simpósio Nacional de Valorização do Cardo was an event that represented avery stimulating meeting and an unequalled opportunity for the scientists dealing with cardoon valorization, within different perspectives. The program covered the most appealing and relevant topics of cardoon research, particularly focus within two major panels: I-The cardoon natural diversity including genetic,morphologic, and technological; II-The biotechnological valorization of cardoon and its added-value opportunities with a concept of circular bioeconomy.(2023/03/23) (2023/03/23 - 2023/03/23)
Symposium (Member of the Organising Committee)
2018/12/05 - 2018/12/06 I Simpósio de valorização do cardo, que tem como objetivo a promoção e divulgação do conhecimento científico e tecnológico em torno da valorização económica do Cardo, resultante da cooperação entre instituições de I&D nacionais e internacionais, bem como com o sector produtivo. (2018/12/05 - 2018/12/06)
Symposium (Member of the Organising Committee)
 Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal
Distinctions

Award

2023 BEST POSTER AWARDS - VIII PhD Students Meeting in Environment and Agriculture
Universidade de Évora, Portugal