Lisete Machado e Silva (821E-7470-0086)

Curriculum Vitae

Lisete Machado e Silva

Lisete is a Researcher at University of Aveiro (UA, Department of Chemistry), Portugal, since April 2019. She is also an Honorary Research Associate in the Glycosciences Laboratory at Imperial College London (ICL). Lisete obtained her BSc degree in Chemistry in 2007 at FCUP (Portugal), and completed her MSc in Chemistry with food specialisation in 2008, at the same University. Her PhD degree in Biochemistry was obtained in 2014 at UA, in collaboration with the GlycoLab: Functional Glycobiology at FCT-NOVA and the Glycosciences Laboratory at ICL. PhD IN BIOCHEMISTRY: Lisete focused on the structural analyses of lipopolysaccharides (LPSs) from clinical isolates of H. pylori by GC-MS and ESI-MSn, and on the development of a bacterial LPS microarray for interaction studies with glycan-binding proteins and human sera. POST-DOCTORAL STUDIES IN GLYCOBIOLOGY AND BIOCHEMISTRY: Her knowledge on the structural (bio)chemistry of complex carbohydrates, combined with her passion for the glycobiology field and expertise on glycan microarrays, have led her to a Research Associate position in the pioneer glycan microarray team (Glycosciences Laboratory) at ICL, in March 2015; and in November 2015, she was named Deputy Microarray Project Leader with responsibility for running, maintaining and troubleshooting the group's microarray equipment and performing glycan array analyses of proteins, received from national and international collaborators. In parallel, Lisete carried out investigations of different liposomal formulations for lipid-linked glycan probes in neoglycolipid (NGL)-based microarrays with respect to the binding signals they elicit in microarray analyses. These studies led to the construction of a new version of a broad-spectrum microarray of ~ 900 natural or synthetic lipid-linked sequence-defined glycan probes (one of the two largest glycan libraries in the world) for the Wellcome-Trust supported Carbohydrate Microarray Facility, suitable for screening analyses of a variety of carbohydrate recognition systems of biological and medical impact and able to serve the scientific community as international resources. During 2015-2019, Lisete actively contributed and provided leadership support to biotechnology and biomedicine R&D projects (translational research); collaborated with 50+ national and international academic and industrial teams that have resulted in > £30k of revenue for facility services. As an Honorary Research Associate in the Glycosciences Laboratory, Lisete is currently providing consultancy advice to the group regarding microarray equipment and training to the new staff members, assisting with microarray experiments, data analysis and preparation of manuscripts. Back to Portugal in 2019, Lisete has been focused on the development of galactomannan-based microparticles, obtained from different natural sources, for pulmonary drug delivery. The structural variability of the different galactomannans is being evaluated using conventional analytical tools that will be complemented with interaction studies with glycan-binding proteins of various recognition systems using glycan microarrays. RESEARCH INTERESTS: 1) glycan structural features and bioactivity relationship; 2) designer and construction of microarrays of natural and/or synthetic glycans; 3) to use glycan microarrays for new ligands discovery and to explore glycan-protein interactions in various biological systems, such as pathogen- and microbiota-host interactions that trigger diseases; 4) development of natural glycan-based products and glycan-based microparticles for drug delivery.

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Identification

Personal identification

Full name: Lisete Machado e Silva
Gender: Female

Citation names

Silva, Lisete M.
Silva, Lisete
Silva, L
Silva, LM

Author identifiers

Ciência ID: 821E-7470-0086
ORCID iD: 0000-0003-4876-5240
Researcher Id: AAD-7415-2020

Email addresses

lisete.silva@ua.pt (Professional)

Addresses

Department of Chemistry, University of Aveiro; Campus Universitário de Santiago, 3810-193, Aveiro, Aveiro, Portugal (Professional)
Glycosciences Laboratory , Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, Burlington Danes Building Hammersmith Campus, Du Cane Rd, W12 0NN, London , London , United Kingdom (Professional)

Websites

http://www.imperial.ac.uk/people/l.machado-e-silva (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Biochemistry

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)

Education

	Degree	Classification
2018/11 Attended	Annual Immunology Short Course for Clinicians and Scientists (Outros) Major in Course Imperial College London, United Kingdom
2018 Attended	Mental Health First Aid course (Outros) Major in Course Imperial College London, United Kingdom
2018 Attended	How Intellectual Property Rights Can Impact Your Research - Course (Outros) Major in Course Imperial College London, United Kingdom
2014 Concluded	Biochemistry (Doutoramento) Major in Biochemistry (clinical branch) Universidade de Aveiro, Portugal "Structural analysis of glycans and development of microarrays from Helicobacter pylori cell surface glycome." (THESIS/DISSERTATION)	Approved by unanimity
2008 Concluded	Chemistry (Mestrado) Major in Food branch Universidade do Porto Faculdade de Ciências, Portugal "Evaluation of the oxidative stability of vegetable oils and olive oil compounds with anti-atherosclerotic activity during food processing. " (THESIS/DISSERTATION)	18/20
2007 Concluded	Chemistry (Licenciatura) Universidade do Porto Faculdade de Ciências, Portugal "Isolation of olive oil compounds with anti-atherosclerotic activity during roasted food processing." (THESIS/DISSERTATION)	14/20

Affiliation

Science

2019/04 - Current	Researcher (Research) Universidade de Aveiro, Portugal
2019 - Current	Visiting Researcher (Research) Imperial College London Glycosciences Laboratory, United Kingdom
2015/03 - 2019/04	Researcher (Research) Imperial College London Glycosciences Laboratory, United Kingdom
2009/05 - 2010/08	Research Trainee (Research) Universidade de Aveiro, Portugal

Teaching in Higher Education

2015 - 2019

Lecturer (University Teacher)

Imperial College London, United Kingdom

Projects

Grant

	Designation	Funders
2018 - 2021/08	GLYCOINTERACT: Glycan-human microbiome interaction: glycan diversity and recognition in the human gut with impact on health and nutrition PTDC/BIA-MIB/31730/2017	Fundação para a Ciência e a Tecnologia
2016 - 2017	Chikungunya Virus Replication and Pathogenesis R01AI123348 Researcher	National Institute of Allergy and Infectious Diseases
2013 - 2014/12	Pylori GlycoArrays: Development of microarrays from Helicobacter pylori cell surface glycome toward immunological and serological studies EXPL/BBB-BQB/0750/2012	Fundação para a Ciência e a Tecnologia

Contract

	Designation	Funders
2019 - 2021/06	PulManCar: Microparticles design for active compounds pulmonary delivery using insulin as model POCI-01-0145-FEDER-029560 Researcher Universidade de Aveiro, Portugal	Fundação para a Ciência e a Tecnologia
2018 - 2019/03	Host-microbiota interactions in the pathology of preterm birth 22-FY18-82 Researcher Imperial College London - Hammersmith Campus, United Kingdom
2015 - 2018/08	Carbohydrate Microarray Facility for Biomedical Exploitation WT108430 Researcher	Wellcome Trust
2017 - 2018/04	Guillain Barre syndrome antibodies during Zika and Chikungunya vaccine trials 87242-544164 Researcher Imperial College London - Hammersmith Campus, United Kingdom	Innovate UK
2015 - 2016/10	Chemo-enzymatic production of specialty glycans BB/M028941/1 Researcher	Biotechnology and Biological Sciences Research Council

Outputs

Publications

Book chapter	Silva, L.; Shahidi, F.; Coimbra, M.A.. "Dried Pears: Phytochemicals and Potential Health Effects". 325-356. 2013. Ferreira, José; Silva, Lisete; Monteiro, Mario; Coimbra, Manuel A.. "Helicobacter pylori cell-surface glycans structural features: Role in gastric colonization, pathogenesis, and carbohydrate-based vaccines". 160-193. 2011. 10.1039/9781849732765-00160 Silva, Lisete M.. "Isolamento e caracterização estrutural de polissacarídeos pécticos da pêra passa de Viseu". In Secagem de Peras. Da tradição à ciência, 205-2014. Viseu, Portugal: CI&DETS, Viseu, 2010. Published
Conference paper	Akune, Yukie; Aspinar, Sena; Silva, Lisete; Stoll, Mark; Palma, Angelina S.; Liu, Yan; Ranzinger, Rene; Feizi, Ten. "CarbArrayART: Carbohydrate Array Analysis and Reporting Tool New software for glycan array for data processing, storage and presentation". Paper presented in Annual Meeting of the Society for Glycobiology, New Orleans, LA, 2018. Published Akune, Yukie; Arpinar, Sena; Stoll, Mark; Silva, Lisete; Palma, Angelina S.; Liu, Yan; Ranzinger, Rene; Feizi, Ten. "New software for glycan array for data processing, storage and presentation". Paper presented in Annual Meeting of the Society for Glycobiology, Portland, OR, 2017. Published Correia, Viviana G.; Brás, Joana Luís Armada; Liu, Yan; Silva, Lisete; Zhang, Yibing; Pinheiro, Benedita; Romão, Maria João C.; et al. "An integrative strategy to decipher glycan recognition in the human gut microbiome.". Paper presented in Annual Meeting of the Society for Glycobiology, New Orleans, LA, 2016. Published Silva, Lisete M.; Childs, R.A.; Palma, Angelina S.; Chai, Wengang; Feizi, Ten; Liu, Yan. "Influence of carrier lipid composition on glycan recognition in NGL-based microarrays.". Paper presented in Annual Meeting of the Society for Glycobiology, San Francisco, CA, 2015. Published
Journal article	Lisete M. Silva; Viviana G. Correia; Ana S.P. Moreira; Maria Rosário M. Domingues; Rui M. Ferreira; Céu Figueiredo; Nuno F. Azevedo; et al. "Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays". Carbohydrate Polymers (2020): 117350-117350. https://doi.org/10.1016/j.carbpol.2020.117350. 10.1016/j.carbpol.2020.117350 Nicole McAllister; Yan Liu; Lisete M. Silva; Anthony J. Lentscher; Wengang Chai; Nian Wu; Kira A. Griswold; et al. "Chikungunya virus strains from each genetic clade bind sulfated glycosaminoglycans as attachment factors". Journal of Virology (2020): https://doi.org/10.1128/JVI.01500-20. 10.1128/JVI.01500-20 Vendele, Ingrida; Willment, Janet A.; Silva, Lisete M.; Palma, Angelina S.; Chai, Wengang; Liu, Yan; Feizi, Ten; et al. "Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls". PLOS Pathogens 16 1 (2020): e1007927. http://dx.doi.org/10.1371/journal.ppat.1007927. 10.1371/journal.ppat.1007927 Wu, N.; Silva, L.M.; Liu, Y.; Zhang, Y.; Gao, C.; Zhang, F.; Fu, L.; et al. "Glycan markers of human stem cells assigned with beam search arrays". Molecular and Cellular Proteomics 18 10 (2019): 1981-2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-85072849227&partnerID=MN8TOARS. 10.1074/mcp.RA119.001309 Chandra, N.; Liu, Y.; Liu, J.-X.; Frängsmyr, L.; Wu, N.; Silva, L.M.; Lindström, M.; et al. "Sulfated glycosaminoglycans as viral decoy receptors for human adenovirus type 37". Viruses 11 3 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85062951333&partnerID=MN8TOARS. 10.3390/v11030247 Rudkin, F.M.; Raziunaite, I.; Workman, H.; Essono, S.; Belmonte, R.; MacCallum, D.M.; Johnson, E.M.; et al. "Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis". Nature Communications 9 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85058242231&partnerID=MN8TOARS. 10.1038/s41467-018-07738-1 Loureiro, L.R.; Sousa, D.P.; Ferreira, D.; Chai, W.; Lima, L.; Pereira, C.; Lopes, C.B.; et al. "Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2–6 sialic acids". Scientific Reports 8 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85051658578&partnerID=MN8TOARS. 10.1038/s41598-018-30421-w Li, Z.; Gao, C.; Zhang, Y.; Palma, A.S.; Childs, R.A.; Silva, L.M.; Liu, Y.; et al. "O-glycome beam search arrays for carbohydrate ligand discovery". Molecular and Cellular Proteomics 17 1 (2018): 121-133. http://www.scopus.com/inward/record.url?eid=2-s2.0-85040257214&partnerID=MN8TOARS. 10.1074/mcp.RA117.000285 Liu, Y.; McBride, R.; Stoll, M.; Palma, A.S.; Silva, L.; Agravat, S.; Aoki-Kinoshita, K.F.; et al. "The minimum information required for a glycomics experiment (MIRAGE) project: Improving the standards for reporting glycan microarray-based data". Glycobiology 27 4 (2017): 280-284. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019624012&partnerID=MN8TOARS. 10.1093/glycob/cww118 Nunes, C.; Silva, L.; Fernandes, A.P.; Guiné, R.P.F.; Domingues, M.R.M.; Coimbra, M.A.. "Occurrence of cellobiose residues directly linked to galacturonic acid in pectic polysaccharides". Carbohydrate Polymers 87 1 (2012): 620-626. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054737677&partnerID=MN8TOARS. 10.1016/j.carbpol.2011.08.027 Silva, Lisete M.; Pinto, Joana; Carrola, Joana; Paiva-Martins, Fátima. "Oxidative stability of olive oil after food processing and comparison with other vegetable oils". Food Chemistry 121 4 (2010): 1177-1187. http://dx.doi.org/10.1016/j.foodchem.2010.02.001. 10.1016/j.foodchem.2010.02.001 Paiva-Martins, F.; Fernandes, J.; Santos, V.; Silva, Lisete; Borges, F.; Rocha, S.; Belo, L.; Santos-Silva, A.. "Powerful Protective Role of 3,4-Dihydroxyphenylethanol-Elenolic Acid Dialdehyde against Erythrocyte Oxidative-Induced Hemolysis". Journal of Agricultural and Food Chemistry 58 1 (2010): 135-140. http://dx.doi.org/10.1021/jf9031052. 10.1021/jf9031052 Silva, L.; Garcia, B.; Paiva-Martins, F.. "Oxidative stability of olive oil and its polyphenolic compounds after boiling vegetable process". LWT - Food Science and Technology 43 9 (2010): 1336-1344. http://www.scopus.com/inward/record.url?eid=2-s2.0-77954027971&partnerID=MN8TOARS. 10.1016/j.lwt.2010.05.013
Thesis / Dissertation	Silva, Lisete Machado e. "Structural analysis of glycans and development of microarrays from Helcobacter pylori cell surface glycome". PhD, 2014. http://hdl.handle.net/10773/15453. Silva, Lisete Machado e. "Determinação da estabilidade de óleos e de compostos com actividade anti-aterosclerótica do azeite durante o processamento de alimentos". Master, 2008. http://hdl.handle.net/10216/64173.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2019/07	The biochemical principles and applications of carbohydrate microarrays	XXVI Portuguese Chemical Society meeting 2019 (Porto, Portugal)
2017	Glycan microarrays in biological and medical research.	12th Meeting of the Portuguese Carbohydrate Group (Glupor 12) Portuguese Carbohydrate Group (Aveiro, Portugal)
2016/06/03	Neoglycolipid-based carbohydrate microarray technology: principles and applications	Universidade de Aveiro (Aveiro, Portugal)
2015	Insights into Helicobacter pylori lipopolysaccharide structural domains using a pathogen carbohydrate microarray.	11th International Meeting of the Portuguese Carbohydrate Group – GLUPOR 11 – and 6th Iberian Carbohydrate Meeting (Viseu, Portugal)
2014	Probing structural domains of H. pylori lipopolysaccharides with glycan binding proteins using microarrays.	Glyco-T 2014, 9th International Symposium on Glycosyltransferases (Porto, Portugal)
2014	Helicobacter pylori cell-surface glycans: Isolation and structural characterization towards development of a novel pathogen microarray.	Glyco-T 2014, 9th International Symposium on Glycosyltransferases. (Porto)
2013	Helicobacter pylori cell surface glycans: Isolation and structural characterization towards development of a novel pathogen microarray.	REQUIMTE research group of Chemistry Department of FCT-UNL, Lisbon
2013	Helicobacter pylori cell-surface glycans: Isolation and structural characterization towards development of a novel pathogen microarray.	GLUPOR 10 and 1st Workshop of Carbohydrates (Covilhã, Portugal)
2013	GC-MS as a tool towards identification of typical sugars occurred in Helicobacter pylori lipopolysaccharides.	8º Encontro Nacional de Cromatografia (Covilhã, Portugal)
2013	Evaluation of the stress-induced growth of the Portuguese clinical isolate Helicobacter pylori CI-5 strain - a structural characterization of cell-surface lipopolysaccharides.	MicroBiotec 13 (Aveiro, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2021/02 - Current	Exploratory assessment of pulse by-products as a source of health promoting ingredients. Co-supervisor	Biochemistry (Degree) Universidade de Aveiro, Portugal
2021/02 - Current	Pomegranate by-products valuation as a source of antioxidants and dietary fibre. Co-supervisor	Biochemistry (Degree) Universidade de Aveiro, Portugal
2021/02 - Current	Exploratory assessment of pulse by-products as a source of health promoting ingredients. Co-supervisor	Biotechnology (Degree) Universidade de Aveiro, Portugal
2020/09 - Current	Study of galactomannan-protein interactions using carbohydrate microarrays Supervisor	Biochemistry (Master) Universidade de Aveiro, Portugal
2020/02 - 2020/07	Aquafaba - uma alternativa vegan às claras do ovo: estudo das propriedades físico-químicas visando a formulação e desenvolvimento de novos produtos. Supervisor	Biochemistry (Degree) Universidade de Aveiro, Portugal
2020/02 - 2020/07	Microcápsulas de levedura para aplicações biomédicas. Co-supervisor	Biochemistry (Degree) Universidade de Aveiro, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2019/10/10 - 2019/10/12	Demonstration of galactomannan-based microparticles for insulin pulmonary delivery Exhibition	TechDays 2019 Universidade de Aveiro, Portugal
2019/09/27 - 2019/09/27	Demonstration of pulmonary insulin delivery using polysaccharide-based carriers. Exhibition	European Researchers’ Night Universidade de Aveiro, Portugal
2018 - 2018	Glycosciences Laboratory (UK)_ Demonstration on how viruses seek out their sugar chain code. "Sugar maker" interactive activities with the public. Festival	White City Mash Up Imperial College London, United Kingdom
2017 - 2017	Glycosciences Laboratory (UK)_Demonstration entitled "Microchips to tell how viruses seek out their sugar chain code" and consisting of: poster, microarray slides demonstration and interactive activities with the public (age 3+) such as "Sugar maker": build "sugar receptors" of different viruses using foam stickers of different shapes and colours; 2018's update of the "Sugar maker" activity: production of sugar chains bracelets, necklaces or keyrings made out of beads to take home. Festival	Imperial College Festival Imperial College London, United Kingdom
2013 - 2013	Helicobacter pylori polysaccharides: occurrence and structural features. Workshop	2nd Workshop in Mass Spectrometry and Carbohydrates Universidade de Aveiro, Portugal

Consulting

	Activity description	Institution / Organization
2021/04 - Current	Providing consultancy advice to the Glycosciences Lab regarding microarray equipment and training to the new staff members, assisting them with glycan microarray experiments and protein binding data analysis.	Imperial College London - Hammersmith Campus, United Kingdom

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2018/12/11 - 2018/12/11	Invited lecture on Glycobiology (MODULE 4: IMMUNE REGULATION) as part of MSc Immunology Course	(Master)	Imperial College London - Hammersmith Campus, United Kingdom
2017/12/12 - 2017/12/12	Invited lecture on Glycobiology (MODULE 4: IMMUNE REGULATION) as part of MSc Immunology Course	(Master)	Imperial College London - Hammersmith Campus, United Kingdom
2015/12/11 - 2015/12/11	Invited lecture on Glycobiology (MODULE 4: IMMUNE REGULATION) as part of MSc Immunology Course	(Master)	Imperial College London - Hammersmith Campus, United Kingdom

Distinctions

Award

2015	Travel award ($500) Society for Glycobiology, United States
2010	4-year post-graduate PhD research fellowship funded by FCT (SFRH/BD/71455/2010) Fundação para a Ciência e a Tecnologia, Portugal