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Lisete is a Researcher at University of Aveiro (UA, Department of Chemistry), Portugal, since April 2019. She is also an Honorary Research Associate in the Glycosciences Laboratory at Imperial College London (ICL). Lisete obtained her BSc degree in Chemistry in 2007 at FCUP (Portugal), and completed her MSc in Chemistry with food specialisation in 2008, at the same University. Her PhD degree in Biochemistry was obtained in 2014 at UA, in collaboration with the GlycoLab: Functional Glycobiology at FCT-NOVA and the Glycosciences Laboratory at ICL. PhD IN BIOCHEMISTRY: Lisete focused on the structural analyses of lipopolysaccharides (LPSs) from clinical isolates of H. pylori by GC-MS and ESI-MSn, and on the development of a bacterial LPS microarray for interaction studies with glycan-binding proteins and human sera. POST-DOCTORAL STUDIES IN GLYCOBIOLOGY AND BIOCHEMISTRY: Her knowledge on the structural (bio)chemistry of complex carbohydrates, combined with her passion for the glycobiology field and expertise on glycan microarrays, have led her to a Research Associate position in the pioneer glycan microarray team (Glycosciences Laboratory) at ICL, in March 2015; and in November 2015, she was named Deputy Microarray Project Leader with responsibility for running, maintaining and troubleshooting the group's microarray equipment and performing glycan array analyses of proteins, received from national and international collaborators. In parallel, Lisete carried out investigations of different liposomal formulations for lipid-linked glycan probes in neoglycolipid (NGL)-based microarrays with respect to the binding signals they elicit in microarray analyses. These studies led to the construction of a new version of a broad-spectrum microarray of ~ 900 natural or synthetic lipid-linked sequence-defined glycan probes (one of the two largest glycan libraries in the world) for the Wellcome-Trust supported Carbohydrate Microarray Facility, suitable for screening analyses of a variety of carbohydrate recognition systems of biological and medical impact and able to serve the scientific community as international resources. During 2015-2019, Lisete actively contributed and provided leadership support to biotechnology and biomedicine R&D projects (translational research); collaborated with 50+ national and international academic and industrial teams that have resulted in > £30k of revenue for facility services. As an Honorary Research Associate in the Glycosciences Laboratory, Lisete is currently providing consultancy advice to the group regarding microarray equipment and training to the new staff members, assisting with microarray experiments, data analysis and preparation of manuscripts. Back to Portugal in 2019, Lisete has been focused on the development of galactomannan-based microparticles, obtained from different natural sources, for pulmonary drug delivery. The structural variability of the different galactomannans is being evaluated using conventional analytical tools and has been complemented with interaction studies with glycan-binding proteins of various recognition systems using glycan microarrays. RESEARCH INTERESTS: 1) glycan structural features and bioactivity relationship; 2) designer and construction of microarrays of natural and/or synthetic glycans; 3) to use glycan microarrays for new ligands discovery and to explore glycan-protein interactions in various biological systems, such as pathogen- and microbiota-host interactions that trigger diseases; 4) development of natural glycan-based products and glycan-based microparticles for drug delivery.
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Identificação

Identificação pessoal

Nome completo
Lisete Machado e Silva
Género
Feminino

Nomes de citação

  • Silva, Lisete M.
  • Silva, Lisete
  • Silva, L
  • Silva, LM

Identificadores de autor

Ciência ID
821E-7470-0086
ORCID iD
0000-0003-4876-5240
Researcher Id
AAD-7415-2020

Endereços de correio eletrónico

  • lisete.silva@ua.pt (Profissional)

Moradas

  • Department of Chemistry, University of Aveiro; Campus Universitário de Santiago, 3810-193, Aveiro, Aveiro, Portugal (Profissional)
  • Glycosciences Laboratory , Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, Burlington Danes Building Hammersmith Campus, Du Cane Rd, W12 0NN, London , London , Reino Unido (Profissional)

Websites

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Bioquímica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2)
Formação
Grau Classificação
2018/11
Frequentou
 Annual Immunology Short Course for Clinicians and Scientists (Outros)
Especialização em Course
Imperial College London, Reino Unido
2018
Frequentou
 Mental Health First Aid course (Outros)
Especialização em Course
Imperial College London, Reino Unido
2018
Frequentou
 How Intellectual Property Rights Can Impact Your Research - Course (Outros)
Especialização em Course
Imperial College London, Reino Unido
2014
Concluído
 Biochemistry (Doutoramento)
Especialização em Biochemistry (clinical branch)
Universidade de Aveiro, Portugal
"Structural analysis of glycans and development of microarrays from Helicobacter pylori cell surface glycome." (TESE/DISSERTAÇÃO)
 Approved by unanimity
2008
Concluído
 Chemistry (Mestrado)
Especialização em Food branch
Universidade do Porto Faculdade de Ciências, Portugal
"Evaluation of the oxidative stability of vegetable oils and olive oil compounds with anti-atherosclerotic activity during food processing. " (TESE/DISSERTAÇÃO)
 18/20
2007
Concluído
 Chemistry (Licenciatura)
Universidade do Porto Faculdade de Ciências, Portugal
"Isolation of olive oil compounds with anti-atherosclerotic activity during roasted food processing." (TESE/DISSERTAÇÃO)
 14/20
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2019/04 - Atual Investigador (Investigação) Universidade de Aveiro, Portugal
2019 - Atual Investigador visitante (Investigação) Imperial College London Glycosciences Laboratory, Reino Unido
Imperial College London Glycosciences Laboratory, Reino Unido
2015/03 - 2019/04 Investigador (Investigação) Imperial College London Glycosciences Laboratory, Reino Unido
2009/05 - 2010/08 Estagiário de Investigação (Investigação) Universidade de Aveiro, Portugal

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento 		Empregador
2015 - 2019 Leitor (Docente Universitário) Imperial College London, Reino Unido
Projetos

Bolsa

Designação Financiadores
2018/09 - 2021/08 GLYCOINTERACT: Glycan-human microbiome interaction: glycan diversity and recognition in the human gut with impact on health and nutrition
PTDC/BIA-MIB/31730/2017
 Fundação para a Ciência e a Tecnologia
Em curso
2016/06 - 2017 Chikungunya Virus Replication and Pathogenesis
R01AI123348
Investigador
National Institute of Allergy and Infectious Diseases
2013/07 - 2014/12 Pylori GlycoArrays: Development of microarrays from Helicobacter pylori cell surface glycome toward immunological and serological studies
EXPL/BBB-BQB/0750/2012
 Fundação para a Ciência e a Tecnologia

Projeto

Designação Financiadores
2019/04/01 - 2022/06/30 PulManCar: Microparticles design for active compounds pulmonary delivery using insulin as model
POCI-01-0145-FEDER-029560
Investigador
Universidade de Aveiro, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2018/04 - 2019/03 Host-microbiota interactions in the pathology of preterm birth
22-FY18-82
Investigador
Imperial College London - Hammersmith Campus, Reino Unido
Em curso
2015/08 - 2018/08 Carbohydrate Microarray Facility for Biomedical Exploitation
WT108430
Investigador
Wellcome Trust
2017/05 - 2018/04 Guillain Barre syndrome antibodies during Zika and Chikungunya vaccine trials
87242-544164
Investigador
Imperial College London - Hammersmith Campus, Reino Unido
 Innovate UK
Concluído
2015/10 - 2016/10 Chemo-enzymatic production of specialty glycans
BB/M028941/1
Investigador
Biotechnology and Biological Sciences Research Council
Produções

Publicações

Artigo em conferência
  1. Akune, Yukie; Aspinar, Sena; Silva, Lisete; Stoll, Mark; Palma, Angelina S.; Liu, Yan; Ranzinger, Rene; Feizi, Ten. "CarbArrayART: Carbohydrate Array Analysis and Reporting Tool New software for glycan array for data processing, storage and presentation". Trabalho apresentado em Annual Meeting of the Society for Glycobiology, New Orleans, LA, 2018.
    Publicado
  2. Akune, Yukie; Arpinar, Sena; Stoll, Mark; Silva, Lisete; Palma, Angelina S.; Liu, Yan; Ranzinger, Rene; Feizi, Ten. "New software for glycan array for data processing, storage and presentation". Trabalho apresentado em Annual Meeting of the Society for Glycobiology, Portland, OR, 2017.
    Publicado
  3. Correia, Viviana G.; Brás, Joana Luís Armada; Liu, Yan; Silva, Lisete; Zhang, Yibing; Pinheiro, Benedita; Romão, Maria João C.; et al. "An integrative strategy to decipher glycan recognition in the human gut microbiome.". Trabalho apresentado em Annual Meeting of the Society for Glycobiology, New Orleans, LA, 2016.
    Publicado
  4. Silva, Lisete M.; Childs, R.A.; Palma, Angelina S.; Chai, Wengang; Feizi, Ten; Liu, Yan. "Influence of carrier lipid composition on glycan recognition in NGL-based microarrays.". Trabalho apresentado em Annual Meeting of the Society for Glycobiology, San Francisco, CA, 2015.
    Publicado
Artigo em revista
  1. Valente, Sara A.; Silva, Lisete M.; Lopes, Guido R.; Sarmento, Bruno; Coimbra, Manuel A.; Passos, Cláudia P.. "Polysaccharide-based formulations as potential carriers for pulmonary delivery - A review of their properties and fates". Carbohydrate Polymers 277 (2022): 118784. http://dx.doi.org/10.1016/j.carbpol.2021.118784.
    Publicado • 10.1016/j.carbpol.2021.118784
  2. Viviana G. Correia; Filipa Trovão; Benedita A. Pinheiro; Joana L. A. Brás; Lisete M. Silva; Cláudia Nunes; Manuel A. Coimbra; et al. "Mapping Molecular Recognition of ß1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus". Microbiology Spectrum 9 3 (2021): https://doi.org/10.1128/Spectrum.01826-21.
    Publicado • 10.1128/Spectrum.01826-21
  3. Li, Chunxia; Palma, Angelina S; Zhang, Pengtao; Zhang, Yibing; Gao, Chao; Silva, Lisete M; Li, Zhen; et al. "Noncovalent microarrays from synthetic amino-terminating glycans: Implications in expanding glycan microarray diversity and platform comparison". Glycobiology 31 8 (2021): 931-946. http://dx.doi.org/10.1093/glycob/cwab037.
    Acesso aberto • Publicado • 10.1093/glycob/cwab037
  4. Lisete M. Silva; Viviana G. Correia; Ana S.P. Moreira; Maria Rosário M. Domingues; Rui M. Ferreira; Céu Figueiredo; Nuno F. Azevedo; et al. Autor correspondente: Lisete M. Silva. "Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays". Carbohydrate Polymers (2020): 117350-117350. https://doi.org/10.1016/j.carbpol.2020.117350.
    Acesso aberto • Publicado • 10.1016/j.carbpol.2020.117350
  5. Nicole McAllister; Yan Liu; Lisete M. Silva; Anthony J. Lentscher; Wengang Chai; Nian Wu; Kira A. Griswold; et al. "Chikungunya virus strains from each genetic clade bind sulfated glycosaminoglycans as attachment factors". Journal of Virology (2020): https://doi.org/10.1128/JVI.01500-20.
    Publicado • 10.1128/JVI.01500-20
  6. Vendele, Ingrida; Willment, Janet A.; Silva, Lisete M.; Palma, Angelina S.; Chai, Wengang; Liu, Yan; Feizi, Ten; et al. "Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls". PLOS Pathogens 16 1 (2020): e1007927. http://dx.doi.org/10.1371/journal.ppat.1007927.
    Publicado • 10.1371/journal.ppat.1007927
  7. Wu, N.; Silva, L.M.; Liu, Y.; Zhang, Y.; Gao, C.; Zhang, F.; Fu, L.; et al. "Glycan markers of human stem cells assigned with beam search arrays". Molecular and Cellular Proteomics 18 10 (2019): 1981-2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-85072849227&partnerID=MN8TOARS.
    Publicado • 10.1074/mcp.RA119.001309
  8. Chandra, N.; Liu, Y.; Liu, J.-X.; Frängsmyr, L.; Wu, N.; Silva, L.M.; Lindström, M.; et al. "Sulfated glycosaminoglycans as viral decoy receptors for human adenovirus type 37". Viruses 11 3 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85062951333&partnerID=MN8TOARS.
    Publicado • 10.3390/v11030247
  9. Rudkin, F.M.; Raziunaite, I.; Workman, H.; Essono, S.; Belmonte, R.; MacCallum, D.M.; Johnson, E.M.; et al. "Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis". Nature Communications 9 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85058242231&partnerID=MN8TOARS.
    Publicado • 10.1038/s41467-018-07738-1
  10. Loureiro, L.R.; Sousa, D.P.; Ferreira, D.; Chai, W.; Lima, L.; Pereira, C.; Lopes, C.B.; et al. "Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2–6 sialic acids". Scientific Reports 8 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85051658578&partnerID=MN8TOARS.
    Publicado • 10.1038/s41598-018-30421-w
  11. Li, Z.; Gao, C.; Zhang, Y.; Palma, A.S.; Childs, R.A.; Silva, L.M.; Liu, Y.; et al. "O-glycome beam search arrays for carbohydrate ligand discovery". Molecular and Cellular Proteomics 17 1 (2018): 121-133. http://www.scopus.com/inward/record.url?eid=2-s2.0-85040257214&partnerID=MN8TOARS.
    Publicado • 10.1074/mcp.RA117.000285
  12. Liu, Y.; McBride, R.; Stoll, M.; Palma, A.S.; Silva, L.; Agravat, S.; Aoki-Kinoshita, K.F.; et al. "The minimum information required for a glycomics experiment (MIRAGE) project: Improving the standards for reporting glycan microarray-based data". Glycobiology 27 4 (2017): 280-284. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019624012&partnerID=MN8TOARS.
    Publicado • 10.1093/glycob/cww118
  13. Nunes, C.; Silva, L.; Fernandes, A.P.; Guiné, R.P.F.; Domingues, M.R.M.; Coimbra, M.A.. "Occurrence of cellobiose residues directly linked to galacturonic acid in pectic polysaccharides". Carbohydrate Polymers 87 1 (2012): 620-626. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054737677&partnerID=MN8TOARS.
    Publicado • 10.1016/j.carbpol.2011.08.027
  14. Silva, Lisete M.; Pinto, Joana; Carrola, Joana; Paiva-Martins, Fátima. "Oxidative stability of olive oil after food processing and comparison with other vegetable oils". Food Chemistry 121 4 (2010): 1177-1187. http://dx.doi.org/10.1016/j.foodchem.2010.02.001.
    Publicado • 10.1016/j.foodchem.2010.02.001
  15. Paiva-Martins, F.; Fernandes, J.; Santos, V.; Silva, Lisete; Borges, F.; Rocha, S.; Belo, L.; Santos-Silva, A.. "Powerful Protective Role of 3,4-Dihydroxyphenylethanol-Elenolic Acid Dialdehyde against Erythrocyte Oxidative-Induced Hemolysis". Journal of Agricultural and Food Chemistry 58 1 (2010): 135-140. http://dx.doi.org/10.1021/jf9031052.
    Publicado • 10.1021/jf9031052
  16. Silva, L.; Garcia, B.; Paiva-Martins, F.. "Oxidative stability of olive oil and its polyphenolic compounds after boiling vegetable process". LWT - Food Science and Technology 43 9 (2010): 1336-1344. http://www.scopus.com/inward/record.url?eid=2-s2.0-77954027971&partnerID=MN8TOARS.
    Publicado • 10.1016/j.lwt.2010.05.013
Capítulo de livro
  1. Silva, Lisete; Shahidi, Fereidoon; Coimbra, Manuel A.. "Dried Pears: Phytochemicals and Potential Health Effects". In Dried Fruits, 325-356. Blackwell Publishing Ltd., 2013.
    Publicado • 10.1002/9781118464663.ch18
  2. Ferreira, José; Silva, Lisete; Monteiro, Mario; Coimbra, Manuel A.. "Helicobacter pylori cell-surface glycans structural features: Role in gastric colonization, pathogenesis, and carbohydrate-based vaccines". 160-193. 2011.
    Publicado • 10.1039/9781849732765-00160
  3. Silva, Lisete M.. "Isolamento e caracterização estrutural de polissacarídeos pécticos da pêra passa de Viseu". In Secagem de Peras. Da tradição à ciência, 205-2014. Viseu, Portugal: CI&DETS, Viseu, 2010.
    Publicado
Tese / Dissertação
  1. Silva, Lisete Machado e. "Structural analysis of glycans and development of microarrays from Helcobacter pylori cell surface glycome". Doutoramento, 2014. http://hdl.handle.net/10773/15453.
  2. Silva, Lisete Machado e. "Determinação da estabilidade de óleos e de compostos com actividade anti-aterosclerótica do azeite durante o processamento de alimentos". Mestrado, 2008. http://hdl.handle.net/10216/64173.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2019/07 The biochemical principles and applications of carbohydrate microarrays XXVI Portuguese Chemical Society meeting 2019
(Porto, Portugal)
2017 Glycan microarrays in biological and medical research. 12th Meeting of the Portuguese Carbohydrate Group (Glupor 12)
Portuguese Carbohydrate Group (Aveiro, Portugal)
2016/06/03 Neoglycolipid-based carbohydrate microarray technology: principles and applications
Universidade de Aveiro (Aveiro, Portugal)
2015 Insights into Helicobacter pylori lipopolysaccharide structural domains using a pathogen carbohydrate microarray. 11th International Meeting of the Portuguese Carbohydrate Group – GLUPOR 11 – and 6th Iberian Carbohydrate Meeting
(Viseu, Portugal)
2014 Probing structural domains of H. pylori lipopolysaccharides with glycan binding proteins using microarrays. Glyco-T 2014, 9th International Symposium on Glycosyltransferases
(Porto, Portugal)
2014 Helicobacter pylori cell-surface glycans: Isolation and structural characterization towards development of a novel pathogen microarray. Glyco-T 2014, 9th International Symposium on Glycosyltransferases.
(Porto)
2013 Helicobacter pylori cell surface glycans: Isolation and structural characterization towards development of a novel pathogen microarray. REQUIMTE research group of Chemistry Department of FCT-UNL, Lisbon
2013 Helicobacter pylori cell-surface glycans: Isolation and structural characterization towards development of a novel pathogen microarray. GLUPOR 10 and 1st Workshop of Carbohydrates
(Covilhã, Portugal)
2013 GC-MS as a tool towards identification of typical sugars occurred in Helicobacter pylori lipopolysaccharides. 8º Encontro Nacional de Cromatografia
(Covilhã, Portugal)
2013 Evaluation of the stress-induced growth of the Portuguese clinical isolate Helicobacter pylori CI-5 strain - a structural characterization of cell-surface lipopolysaccharides. MicroBiotec 13
(Aveiro, Portugal)

Orientação

Título / Tema
Papel desempenhado 		Curso (Tipo)
Instituição / Organização
2020/09 - 2021/12/06 Interaction study between carob galactomannans and proteins using carbohydrate microarray tecnology
Orientador
 Biochemistry (Mestrado)
Universidade de Aveiro, Portugal
2021/02 - 2021/09/17 Água da demolha: o subproduto das leguminosas.
Coorientador
 Biochemistry (Licenciatura/Bacharelato)
Universidade de Aveiro, Portugal
2021/02 - 2021/07/23 Improving aquafaba production: The effect of sodium bicarbonate.
Coorientador
 Biotechnology (Licenciatura/Bacharelato)
Universidade de Aveiro, Portugal
2020/02 - 2020/07 Aquafaba - uma alternativa vegan às claras do ovo: estudo das propriedades físico-químicas visando a formulação e desenvolvimento de novos produtos.
Orientador
 Biochemistry (Licenciatura/Bacharelato)
Universidade de Aveiro, Portugal
2020/02 - 2020/07 Microcápsulas de levedura para aplicações biomédicas.
Coorientador
 Biochemistry (Licenciatura/Bacharelato)
Universidade de Aveiro, Portugal

Participação em evento

Descrição da atividade
Tipo de evento 		Nome do evento
Instituição / Organização
2019/10/10 - 2019/10/12 Demonstration of galactomannan-based microparticles for insulin pulmonary delivery
Exposição
 TechDays 2019
Universidade de Aveiro, Portugal
2019/09/27 - 2019/09/27 Demonstration of pulmonary insulin delivery using polysaccharide-based carriers.
Exposição
 European Researchers’ Night
Universidade de Aveiro, Portugal
2018 - 2018 Glycosciences Laboratory (UK)_ Demonstration on how viruses seek out their sugar chain code. "Sugar maker" interactive activities with the public.
Festival
 White City Mash Up
Imperial College London, Reino Unido
2017 - 2017 Glycosciences Laboratory (UK)_Demonstration entitled "Microchips to tell how viruses seek out their sugar chain code" and consisting of: poster, microarray slides demonstration and interactive activities with the public (age 3+) such as "Sugar maker": build "sugar receptors" of different viruses using foam stickers of different shapes and colours; 2018's update of the "Sugar maker" activity: production of sugar chains bracelets, necklaces or keyrings made out of beads to take home.
Festival
 Imperial College Festival
Imperial College London, Reino Unido
2013 - 2013 Helicobacter pylori polysaccharides: occurrence and structural features.
Oficina (workshop)
 2nd Workshop in Mass Spectrometry and Carbohydrates
Universidade de Aveiro, Portugal

Consultoria / Parecer

Descrição da atividade Instituição / Organização
2019/04 - Atual Providing consultancy advice to the Glycosciences Lab regarding microarray equipment and training to the new staff members, assisting them with glycan microarray experiments and protein binding data analysis. Imperial College London - Hammersmith Campus, Reino Unido
2018 - Atual Peer-reviewing in international journals: Carbohydrate Polymers, Molecular Oncology and Pharmaceutical Biology.

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2021/03 - 2021/07 Practical modules of the Curricular Unit of General Chemistry (Licenciatura) Universidade de Aveiro, Portugal
2021/05/13 - 2021/05/14 Training course on Glycan Microarrays: practical aspects of neoglycolipid (NGL)-based microarrays. Glycosciences Laboratory, Imperial College London
2018/12/11 - 2018/12/11 Invited lecture on Glycobiology (MODULE 4: IMMUNE REGULATION) as part of MSc Immunology Course (Master) Imperial College London - Hammersmith Campus, Reino Unido
2017/12/12 - 2017/12/12 Invited lecture on Glycobiology (MODULE 4: IMMUNE REGULATION) as part of MSc Immunology Course (Master) Imperial College London - Hammersmith Campus, Reino Unido
2015/12/11 - 2015/12/11 Invited lecture on Glycobiology (MODULE 4: IMMUNE REGULATION) as part of MSc Immunology Course (Master) Imperial College London - Hammersmith Campus, Reino Unido

Membro de comissão

Descrição da atividade
Tipo de participação 		Instituição / Organização
2021 - Atual Member of Thesis Advisory Committee of the PhD student Viviana G. Correia.
Consultor
Distinções

Prémio

2015 Travel award ($500)
Society for Glycobiology, Estados Unidos
2010 4-year post-graduate PhD research fellowship funded by FCT (SFRH/BD/71455/2010)
Fundação para a Ciência e a Tecnologia, Portugal