Cristiana Pires (CP) completed her graduation in Medicinal Chemistry at University of Coimbra in 2018. In that same year, she has received a PhD grant in Chemistry, with a specialization in Biological Chemistry, also at the University of Coimbra, enabling the initiation of a new research project. Throughout her journey, she has developing skills in cell culture techniques, and the characterization of in vitro cell monolayers designed to mimic biological barriers such as Caco-2 and MDCK cell lines. Additionally, she has gathered experience in the determination of compounds permeability through these cell monolayers. Currently, CP research interest are focused on improving the accuracy and reliability of permeability data obtained from these in vitro models, thereby facilitating their use into in silico methodologies for predicting permeability based on molecular structure. In pursuit of this objective, she has undertaken a systematically review and evaluation of the several sources contributing for the permeability data variability in assays using the cell monolayers. In a way to find strategies, she has proposed the re-utilization of cell monolayers for additional permeability assays as a strategic approach that has the potential to increase the throughput of the permeability assessments. The initial validation studies of this new methodology has resulted in the publication of a scientific paper. Furthermore, CP has actively engaged in collaborative research projects on-going in her research group, particularly focused in the study of the bioavailability of bioactive compounds from natural plant extracts connected with the concept of functional food. Her substantive contributions to these collective projects have been recognized through her co-authorship of five scientific publications, thereby highlighting her multifaceted expertise. Technical skills >> Cell Culture; Permeability assays; Toxicity assays; High-Performance Liquid Chromatography; Confocal Laser Scanning Microscopy