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Cristiana Pires (CP) completed her graduation in Medicinal Chemistry at University of Coimbra in 2018. In that same year, she has received a PhD grant in Chemistry, with a specialization in Biological Chemistry, also at the University of Coimbra, enabling the initiation of a new research project. Throughout her journey, she has developing skills in cell culture techniques, and the characterization of in vitro cell monolayers designed to mimic biological barriers such as Caco-2 and MDCK cell lines. Additionally, she has gathered experience in the determination of compounds permeability through these cell monolayers. Currently, CP research interest are focused on improving the accuracy and reliability of permeability data obtained from these in vitro models, thereby facilitating their use into in silico methodologies for predicting permeability based on molecular structure. In pursuit of this objective, she has undertaken a systematically review and evaluation of the several sources contributing for the permeability data variability in assays using the cell monolayers. In a way to find strategies, she has proposed the re-utilization of cell monolayers for additional permeability assays as a strategic approach that has the potential to increase the throughput of the permeability assessments. The initial validation studies of this new methodology has resulted in the publication of a scientific paper. Furthermore, CP has actively engaged in collaborative research projects on-going in her research group, particularly focused in the study of the bioavailability of bioactive compounds from natural plant extracts connected with the concept of functional food. Her substantive contributions to these collective projects have been recognized through her co-authorship of five scientific publications, thereby highlighting her multifaceted expertise. Technical skills >> Cell Culture; Permeability assays; Toxicity assays; High-Performance Liquid Chromatography; Confocal Laser Scanning Microscopy
Identificação

Identificação pessoal

Nome completo
Cristiana Lages Pires

Nomes de citação

  • Pires, Cristiana L.

Identificadores de autor

Ciência ID
591E-46F5-47CC
ORCID iD
0000-0002-9098-5848

Domínios de atuação

  • Ciências Naturais
  • Ciências Naturais - Ciências Biológicas - Biofísica
  • Ciências Exatas - Química - Química Analítica
  • Ciências Médicas e da Saúde - Medicina Básica - Química Médica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Inglês Utilizador independente (B2) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador independente (B2)
Português (Idioma materno)
Formação
Grau Classificação
2018/09/01 - 2024
Em curso
PhD student in Chemsitry, specialization in Biological Chemistry (Doutoramento)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"IMPROVING THE ACCURACY OF PERMEABILITY DATA TO GAIN PREDICTIVE POWER: ASSESSING SOURCES OF VARIABILITY IN ASSAYS USING CELL MONOLAYERS" (TESE/DISSERTAÇÃO)
2015 - 2018
Concluído
Master of Science in Medicinal Chemistry (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Permeability through Caco-2 cell monolayers as a model for BBB: implementation and preliminary evaluation using model compounds" (TESE/DISSERTAÇÃO)
18
2015
Concluído
Bachelor in Medicinal Chemistry (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Thermal Denaturation of protein Transthyretin in the presence of Guanidine Thiocyanate" (TESE/DISSERTAÇÃO)
15
Percurso profissional

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2018/09/01 - 2024 PhD Fellow Universidade de Coimbra Centro de Química de Coimbra, Portugal
Universidade de Coimbra Centro de Química de Coimbra, Portugal
2018/04/02 - 2018/08/02 Research Fellow of the FCT-financed project "Unraveling the Rules of Passive Permeation through the Blood-Brain Barrier" Universidade de Coimbra Centro de Química de Coimbra, Portugal
Universidade de Coimbra Centro de Química de Coimbra, Portugal
2017/09/13 - 2018/02/28 Scientific internship in Biological Membranes Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2015/02/15 - 2015/06/08 Scientific Traineeship in Proteins Stability Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
Projetos

Bolsa

Designação Financiadores
2018/09/01 - 2024/09/01 IMPROVING THE ACCURACY OF PERMEABILITY DATA TO GAIN PREDICTIVE POWER: ASSESSING SOURCES OF VARIABILITY IN ASSAYS USING CELL MONOLAYERS
SFRH/BD/138873/2018
Bolseiro de Doutoramento
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2018/04/02 - 2018/08/02 Unraveling the Rules of Passive Permeation through the Blood-Brain Barrier
PTDC/DTPFTO/2784/2014
Bolseiro de Investigação
Universidade de Coimbra, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em conferência
  1. Cristiana L. Pires; Susana F. Nascimento; Elsa T. Rodrigues; Lia P. Godinho; Catarina Churro; Miguel A. Pardal; Maria João Moreno. "Bioavailability and Biotransformation of Paralytic Shellfish Toxins Assessed by Permeability Assays Using Caco-2 Monolayers". 2022.
    10.3390/BiTaP-12880
Artigo em revista
  1. Iris Correia; Madalena Antunes; Carla Tecelão; Marta Neves; Cristiana L. Pires; Pedro F. Cruz; Maria Rodrigues; et al. "Nutritive Value and Bioactivities of a Halophyte Edible Plant: Crithmum maritimum L. (Sea Fennel)". Plants (2024): https://doi.org/10.3390/plants13030427.
    10.3390/plants13030427
  2. Cristiana L. Pires; Inês M. V. Silva; Manuel A. Coimbra; Maria João Moreno; Filipe Coreta-Gomes. "Effect of Coffee on the Bioavailability of Sterols". Foods (2022): https://doi.org/10.3390/foods11192935.
    10.3390/foods11192935
  3. Primitivo, Maria João; Neves, Marta; Pires, Cristiana L.; Cruz, Pedro F.; Brito, Catarina; Rodrigues, Ana C.; de Carvalho, Carla C.C.R.; et al. "Edible flowers of Helichrysum italicum: Composition, nutritive value, and bioactivities". Food Research International 157 (2022): 111399. http://dx.doi.org/10.1016/j.foodres.2022.111399.
    10.1016/j.foodres.2022.111399
  4. Cristiana L. Pires; Catarina Praça; Patrícia A. T. Martins; Ana Batista de Carvalho; Lino Ferreira; MPM Marques; Maria João Pedrosa Ferreira Moreno Silvestre. "Re-Use of Caco-2 Monolayers in Permeability Assays-Validation Regarding Cell Monolayer Integrity". Pharmaceutics (2021): https://www.mdpi.com/1999-4923/13/10/1563.
    10.3390/pharmaceutics13101563
  5. Rodrigues, Elsa T.; Nascimento, Susana F.; Pires, Cristiana L.; Godinho, Lia P.; Churro, Catarina; Moreno, Maria João; Pardal, Miguel A.. "Determination of intestinal absorption of the paralytic shellfish toxin GTX-5 using the Caco-2 human cell model". Environmental Science and Pollution Research (2021): http://dx.doi.org/10.1007/s11356-021-15342-y.
    Aceite para publicação • 10.1007/s11356-021-15342-y
  6. Brito, Catarina; Bertotti, Taciana; Primitivo, Maria João; Neves, Marta; Pires, Cristiana L.; Cruz, Pedro F.; Martins, Patrícia A.T.; et al. "Corema album spp: Edible wild crowberries with a high content in minerals and organic acids". Food Chemistry 345 (2021): 128732. http://dx.doi.org/10.1016/j.foodchem.2020.128732.
    Acesso aberto • Aceite para publicação • 10.1016/j.foodchem.2020.128732
  7. Martins, Patrícia A.T.; Domingues, Neuza; Pires, Cristiana; Alves, Ana Maria; Palmeira, Tiago; Samelo, Jaime; Cardoso, Renato; Velazquez-Campoy, Adrian; Moreno, Maria João. "Molecular crowding effects on the distribution of amphiphiles in biological media". Colloids and Surfaces B: Biointerfaces 180 (2019): 319-325. http://dx.doi.org/10.1016/j.colsurfb.2019.04.065.
    Publicado • 10.1016/j.colsurfb.2019.04.065
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2022/09/08 Bioavailability and biotransformation of Paralytic Shellfish Toxins assessed by permeability assays using human intestinal Caco-2 cell monolayers Biosystems in Toxicology and Pharmacology – Current challenges
ESTG-Polytechnic Institute of Leiria (Portugal)

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2023/03/06 - 2023/03/09 Presentation of a poster entitled: Re-use of Caco-2 monolayers in permeability assays: validation regarding cell monolayer integrity, biochemistry, and function
Congresso
ACS publications symposium| Biological and Medicinal chemistry
American Chemical Society, Estados Unidos
2022/06/20 - 2022/06/21 Presentation of a poster entitled: Validation of the re-use of Caco-2 monolayers for multiple permeability assays
Congresso
8th International Iberian Biophysics Congress
Spanish Biophysical Society, Espanha
2021/06/14 - 2021/06/16 Presentation of a poster entitled: Re-use of Caco-2 monolayers in permeability assays – validation regarding cell monolayer integrity
Congresso
7th International Iberian Biophysics Congress
Portuguese Biophysical Society, Portugal
2019/10/11 - 2019/10/13 Workshop on Biological Chemistry 2019 , covering recent scientific developments on Lipid/Protein interactions and their importance for Life
Encontro
WBC 2019 - Lipids & Proteins - Interactions for Life
Universidade de Coimbra, Portugal
2018/02/16 - 2018/02/17 Mini-symposium on P-glycoprotein: the role of P-glycoprotein in cancer drug resistance (ABC transporters)
Simpósio
Mini-symposium on P-glycoprotein
Universidade de Coimbra Departamento de Química, Portugal
2017/04/07 - 2017/04/10 V ENEQUI -Encontro Nacional de Estudantes de Química
Encontro
V ENEQUI -Encontro Nacional de Estudantes de Química
Universidade de Coimbra, Portugal
2014/11/06 - 2014/11/07 National congress covering scientific discussions about brain disorders such as Parkinson, Alzheimer’s and depression
Congresso
VIII Scientific Congress of the NEF/AAC: "Brain and Dysfunctions"
Universidade de Coimbra Faculdade de Farmácia, Portugal

Membro de associação

Nome da associação Tipo de participação
2021 - Atual Portuguese Biophysics Society Member
Distinções

Prémio

2018 PhD Fellowship - SFRH/BD/138873/2018
Fundação para a Ciência e a Tecnologia, Portugal