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||AIM: The aim of Miguel Castanho Lab is to unravel the physical principles that determine peptide/protein-lipid interactions, with implications in viral fusion (eg HIV, Dengue, Zika, Measles), antimicrobial action, including biofilms, and CNS-targeting (translocation of the blood-brain barrier, BBB, in analgesia and neuroprotection). ||MAIN PARADIGM-SHIFT ACHIEVEMENTS AND DISRUPTIVE LINES OF WORK: > Viral fusion – We established that viral fusion inhibitors for HIV and Measles interact with membranes as part of their mode of action and self-association events are key for their efficacy (eg JACS 2004, 126, 14758-14763; JACS 2008, 130, 6215-6223; J Virol 2017, 91, e01554-16; ACS Nano 2018, 12, 9855-9865). | > Translocation over the BBB – Cell penetrating peptides from viral protein domain templates that use receptor-free routes are now used as efficient carriers to shuttle drugs and antibodies to the brain (eg Biochemistry 2005, 44, 10189-10198; Bioinformatics 2015, 31, 2252-2256; ACS Chemical Biology 2017, 12, 1257-1268). | > Antibacterial action – We bridged molecular biophysics of antimicrobial peptides studies in artificial systems to microbiology as we found how to quantitatively estimate biological activity from biophysical parameters (eg Biochimica et Biophysica Acta -Biomembranes 2007, 1768, 1277-1290; Nature Reviews Microbiology 2009, 7, 245-250; J Biol Chem 2010, 285, 27536-27544; J Antimicrobial Chemotherapy 2019, dkz223). | > Analgesia and neuroprotection – Endogenous neuropeptides were modified so they could traverse the BBB and be used in analgesia and neuroprotection therapies (eg British J Pharmacol 2011, 163, 964-973; Molecular pharmaceutics 2011, 1929-1940; Frontiers in aging neuroscience 2013, 5, 68; ACS Chemical Neuroscience 2017, 8, 1663-1667). | > Quantifying and imaging drug-lipid interactions – We have developed tailored methodologies based on spectroscopic techniques, such as fluorescence emission, SPR, and light scattering with zeta-potential, and AFM to quantify retention of drugs in lipid structures and unravel the structures emerging therefrom (eg Analytical Biochemistry 2002,307, 1-12; BBA-Biomembranes 2003; 1612; 123-135; Trends Pharm Sciences 2010, 31, 449-454; Eur Biophys J 2011, 40, 481-487; BBA-Biomembranes 2015, 1848, 554-560; Frontiers in microbiology 2017, 8, 775). ||TRANSLATION TO INDUSTRY: Encompassing the achievements in academic research, Castanho’s lab has engaged with industry for drug development in the fields of Pain (2 patents), neuroprotection (2 patents), and viral infection (1 patent). 3 out of the 5 patents were filed under agreement with industrial partners. | M Castanho coordinated the consortia of 2 major academia-industry projects funded by the EU. Currently, he coordinates an EIC (European Innovation Council) FET-OPEN academia-industry consortium for antiviral drug development. ||SERVICE TO SOCIETY M Castanho, among other appointments, has been the president of the Portuguese Biochemical Society, and member of working groups in FEBS and PABMB. He has also contributed to the organization of major international meetings (eg FEBS2012, 14th IUBMB Conference, Chairman of the IUBMB-FEBS-PABMB 2021 congress). In addition, he contributes regularly to the general press and mass media with opinion articles as advocate for Science. | Chief Editor for Pharmaceutical Innovations/Frontiers in Medical Technologies, and editorial board member of J. Peptide Sci, and Peptide Sci. || INVITATIONS TO MAJOR INTERNATIONAL MEETINGS: Invited plenary/keynote speaker for major international meetings in Biophysics and Peptide Science, such as: 33rd European Peptide Symposium, 22nd American Peptide Symposium, 9th European Biophysics Congress. ||BIBLIOMETRIC DATA March 2020: 178 papers in international prestige journals, H= 40-46, 5 books as main co-editor. Remark: poster and short oral communications not listed totally below to avoid the burden of information overload
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Identificação

Identificação pessoal

Nome completo
Miguel Castanho

Nomes de citação

  • Castanho, Miguel

Identificadores de autor

Ciência ID
0C12-9932-77FB
ORCID iD
0000-0001-7891-7562

Moradas

  • Faculty of Medicine - Institute of Molecular Medicine and Biochemistry Department, University of Lisbon. , School of Medicine, Edifício Egas Moniz, 1649-028, Lisboa, Lisboa, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Médicas e da Saúde - Ciências da Saúde
  • Ciências Naturais - Ciências Biológicas - Biofísica
  • Ciências Sociais - Ciências da Comunicação
  • Ciências Médicas e da Saúde - Ciências da Saúde
  • Ciências Médicas e da Saúde - Ciências da Saúde
  • Ciências Médicas e da Saúde - Ciências da Saúde
Formação
Grau Classificação
1999
Concluído
 Título de Agregado (Título de Agregado)
Universidade de Lisboa Faculdade de Ciências, Portugal
 Cum laude
1993
Concluído
 Bioquímica Física / Biofísica Molecular / Química Biofísica (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"A model for the biochemical action of the antibiotic Filipin" (TESE/DISSERTAÇÃO)
 Cum laude
1990
Concluído
 Biochemistry (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Interacção polienos-colesterol em bicamadas lipídicas" (TESE/DISSERTAÇÃO)
 18/20
Percurso profissional

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento 		Empregador
2007/09/01 - Atual Professor Catedrático (Docente Universitário) Universidade de Lisboa Faculdade de Medicina, Portugal
2002/05/15 - 2007/08/31 Professor Associado (Docente Universitário) Universidade de Lisboa Faculdade de Ciências, Portugal
1994/11/15 - 2002/05/15 Professor Auxiliar (Docente Universitário) Universidade de Lisboa Faculdade de Ciências, Portugal

Outros

Categoria Profissional
Instituição de acolhimento 		Empregador
2007/09/01 - Atual Director - Institute of Biochemistry, Faculty of Medicine, University of Lisbon. Universidade de Lisboa Faculdade de Medicina, Portugal
2016/02/10 - 2017/09/14 Vice-President Fundação para a Ciência e a Tecnologia, Portugal
2011/08/10 - 2016/02/10 Vice-dean Universidade de Lisboa Faculdade de Medicina, Portugal
2006/01/01 - 2007/08/31 Vice-president - Scientific Council - Faculty of Sciences, Univ. Lisbon Universidade de Lisboa Faculdade de Ciências, Portugal
Projetos

Bolsa

Designação Financiadores
2022/01/01 - 2025/12/31 BREAST-BRAIN-N-BBB: Protecting the brain from metastatic breast cancer.
Grant HR21-00605
Investigador responsável
Instituto de Medicina Molecular, Portugal

CIC biomaGUNE, Espanha

Biodonostia Instituto de Investigación Sanitaria, Espanha

Universidade de Lisboa Faculdade de Farmácia, Portugal
Obra Social La Caixa

Fundação para a Ciência e a Tecnologia
Em curso
2019 - 2024 NOVIRUSES2BRAIN: ''One size fits all'' unique drug to eradicate multiple viral species simultaneously from the central nervous system of co-infected individual
Grant agreement No 828774
Investigador responsável
Instituto de Medicina Molecular, Portugal
 Horizon 2020 European Innovation Council Fast Track to Innovation
Em curso
2014 - 2021 M2b-PhD: A multinstitutional doctoral program on Medical Biochemistry and Biophysics
PD/00473/2012
Investigador responsável
Universidade de Lisboa Faculdade de Medicina, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2017/10/02 - 2019 Cell-cell communication in metastatic breast cancer: a control point that can lead to breast cancer treatment
PTDC/BBB-BQB/1693/2014
Investigador responsável
Fundação para a Ciência e a Tecnologia
2015 - 2019 INPACT: Innovative peptides against cancer and pathogenic bacteria, with advances in science, biopharmaceutical drug development, product market targeting, training , and communication
Proposal number: 644167
Investigador responsável
Instituto de Medicina Molecular, Portugal
 Research Executive Agency
Concluído
2015/02/01 - 2018 Engineering protease-resistant alpha-beta peptides for broad-spectrum antivirals
info:eu-repo/grantAgreement/NIH/NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES/1R01AI114736-01A1/US
Instituto de Medicina Molecular, Portugal
 National Institutes of Health
Concluído
2014/03 - 2017/02 DELIN: An innovative approach to deliver dual-targeting HIV entry inhibitors using cationic liposomes
135844
Provided by PTCRIS: 135844
Investigador responsável
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluído
2011 - 2015 PPS3 - Peptides and antibodies in Alzheimer research
NA
Investigador responsável
2011/03 - 2014/08 Membrane fusion mechanism of Influenza Hemagglutinin: a simulation and biophysical approach.
114774
Provided by PTCRIS: 114774
Investigador
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
2011/03 - 2014/08 LIPIDENGUE: The role of hepatic lipid membranes and lipid droplets in Dengue virus encapsidation. A gateway to new therapies.
112929
Provided by PTCRIS: 112929
Investigador responsável
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluído
2009 - 2013 PEP2BRAIN: Selected peptides as drug candidates directed to pain and neurodegeneration
Grant agreement no.: 230654
Investigador responsável
Research Executive Agency
Concluído
2009/01/01 - 2012 Elucidating the mechanism of action of cyclotides: ultra-stable proteins from plants
info:eu-repo/grantAgreement/EC/FP7/220318/EU
Investigador responsável
European Commission
2009 - 2012 MEMPEPACROSS: Membrane-active peptides across disciplines and continents: An integrated approach to find new strategies to fight bacteria, dengue virus and neurodegeneration
NA
Investigador responsável
2007/09 - 2011/01 Targeting effective kyotorphin derivatives to the brain
69493
Provided by PTCRIS: 69493
 Fundação para a Ciência e a Tecnologia, I.P.
2008 - 2011 Unravelling the mechanism of action of membrane-active peptides combining NMR and optical spectroscopies
NA
Investigador
2008 - 2011 Project 4.1.3/CAPES/CPLP joint project with the Lab. Biochemistry of Virus, Federal University Rio de Janeiro, Brazil
NA
Investigador responsável
2005 - 2008 An atomic force microscopy facility devoted to biomedical and biochemical studies at the molecular-level
Project REEQ/140/BIO/2005
Investigador responsável
1998 - 2001 The interaction of the fusion peptide of the HIV-1 membrane protein, gp41, with biomembranes and its inhibition
PRAXISXXI/P/SAU/14025/1998
Investigador responsável
1998 - 2001 Structural details of the mode of action of the fusion peptide of the HIV-1 membrane protein gp41 and its analogues
PRAXIS/P/BIO/12049/1998
Investigador responsável
1996 - 1999 Structural study of the interaction between Influenza´s Haemagglutinin and a peptide derived from gp41 (HIV-1) with model systems of membranes
PRAXISXXI/PCNA/BIO/56/96
Investigador responsável

Projeto

Designação Financiadores
2019 - 2023 VectorB2B: Drug Developing - new company gathering strengths, building capacity, innovating products, and far fetching in Drug Discovery and Development services
VectorB2B
Investigador responsável
Universidade de Lisboa Faculdade de Medicina, Portugal

TechnoPhage SA, Portugal

Universidade de Coimbra, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
Produções

Publicações

Artigo em revista
  1. Mota-Silva, Iara; Castanho, Miguel A. R. B.; Silva-Herdade, Ana Santos. "Towards Non-Invasive Intravital Microscopy: Advantages of Using the Ear Lobe Instead of the Cremaster Muscle". Life 13 4 (2023): 887. http://dx.doi.org/10.3390/life13040887.
    10.3390/life13040887
  2. André, Ana S.; Dias, Joana N. R.; Aguiar, Sandra; Nogueira, Sara; Bule, Pedro; Carvalho, Joana Inês; António, João P. M.; et al. "Rabbit derived VL single-domains as promising scaffolds to generate antibody–drug conjugates". Scientific Reports 13 1 (2023): http://dx.doi.org/10.1038/s41598-023-31568-x.
    10.1038/s41598-023-31568-x
  3. Vieira-da-Silva, Beatriz; Castanho, Miguel A. R. B.. "Resazurin Reduction-Based Assays Revisited: Guidelines for Accurate Reporting of Relative Differences on Metabolic Status". Molecules 28 5 (2023): 2283. http://dx.doi.org/10.3390/molecules28052283.
    10.3390/molecules28052283
  4. Strandberg, Erik; Wadhwani, Parvesh; Bürck, Jochen; Anders, Patrick; Mink, Christian; van den Berg, Jonas; Ciriello, Raffaele A. M.; et al. "Temperature-Dependent Re-alignment of the Short Multifunctional Peptide BP100 in Membranes Revealed by Solid-State NMR Spectroscopy and Molecular Dynamics Simulations". ChemBioChem 24 4 (2023): http://dx.doi.org/10.1002/cbic.202200602.
    10.1002/cbic.202200602
  5. Vieira-da-Silva, Beatriz; Castanho, Miguel A. R. B.. "The structure and matrix dynamics of bacterial biofilms as revealed by antimicrobial peptides' diffusion". Journal of Peptide Science (2023): http://dx.doi.org/10.1002/psc.3470.
    10.1002/psc.3470
  6. Sousa, Bárbara B.; de Almeida, Cátia Rebelo; Barahona, Ana F.; Lopes, Raquel; Martins-Logrado, Ana; Cavaco, Marco; Neves, Vera; et al. "Selective Inhibition of Bruton’s Tyrosine Kinase by a Designed Covalent Ligand Leads to Potent Therapeutic Efficacy in Blood Cancers Relative to Clinically Used Inhibitors". ACS Pharmacology & Translational Science 5 11 (2022): 1156-1168. http://dx.doi.org/10.1021/acsptsci.2c00163.
    10.1021/acsptsci.2c00163
  7. Dias, Susana A; Pinto, Sandra N; Silva-Herdade, Ana S; Cheneval, Olivier; Craik, David J; Coutinho, Ana; Castanho, Miguel A R B; Henriques, Sónia T; Veiga, Ana Salomé. "A designed cyclic analogue of gomesin has potent activity against Staphylococcus aureus biofilms". Journal of Antimicrobial Chemotherapy 77 12 (2022): 3256-3264. http://dx.doi.org/10.1093/jac/dkac309.
    10.1093/jac/dkac309
  8. Valério, Mariana; Mendonça, Diogo A.; Morais, João; Buga, Carolina C.; Cruz, Carlos H.; Castanho, Miguel A.R.B.; Melo, Manuel N.; et al. "Parainfluenza Fusion Peptide Promotes Membrane Fusion by Assembling into Oligomeric Porelike Structures". ACS Chemical Biology 17 7 (2022): 1831-1843. http://dx.doi.org/10.1021/acschembio.2c00208.
    10.1021/acschembio.2c00208
  9. Todorovski, Toni; Mendonça, Diogo A.; Fernandes-Siqueira, Lorena O.; Cruz-Oliveira, Christine; Guida, Giuseppina; Valle, Javier; Cavaco, Marco; et al. "Targeting Zika Virus with New Brain- and Placenta-Crossing Peptide–Porphyrin Conjugates". Pharmaceutics 14 4 (2022): 738. http://dx.doi.org/10.3390/pharmaceutics14040738.
    10.3390/pharmaceutics14040738
  10. Cavaco, Marco; Castanho, Miguel A. R. B.; Neves, Vera. "The Use of Antibody-Antibiotic Conjugates to Fight Bacterial Infections". Frontiers in Microbiology 13 (2022): http://dx.doi.org/10.3389/fmicb.2022.835677.
    10.3389/fmicb.2022.835677
  11. Caccuri, Francesca; Neves, Vera; Gano, Lurdes; Correia, João D. G.; Oliveira, Maria Cristina; Mazzuca, Pietro; Caruso, Arnaldo; Castanho, Miguel. "The HIV-1 Matrix Protein p17 Does Cross the Blood-Brain Barrier". Journal of Virology 96 1 (2022): http://dx.doi.org/10.1128/jvi.01200-21.
    10.1128/jvi.01200-21
  12. Marco Cavaco; Silvia Frutos; Paula Oliete; Javier Valle; David Andreu; Miguel A. R. B. Castanho; Miquel Vila-Perelló; Vera Neves. "Conjugation of a Blood Brain Barrier Peptide Shuttle to an Fc Domain for Brain Delivery of Therapeutic Biomolecules". ACS Medicinal Chemistry Letters (2021): https://doi.org/10.1021/acsmedchemlett.1c00225.
    10.1021/acsmedchemlett.1c00225
  13. Oliveira, Filipa D.; Cavaco, Marco; Figueira, Tiago N.; Valle, Javier; Neves, Vera; Andreu, David; Gaspar, Diana; Castanho, Miguel A. R. B.. "The antimetastatic breast cancer activity of the viral protein-derived peptide vCPP2319 as revealed by cellular biomechanics". The FEBS Journal 289 6 (2021): 1603-1624. http://dx.doi.org/10.1111/febs.16247.
    10.1111/febs.16247
  14. Cavaco, Marco; Fraga, Patrícia; Valle, Javier; Andreu, David; Castanho, Miguel A. R. B.; Neves, Vera. "Development of Breast Cancer Spheroids to Evaluate Cytotoxic Response to an Anticancer Peptide". Pharmaceutics 13 11 (2021): 1863. http://dx.doi.org/10.3390/pharmaceutics13111863.
    10.3390/pharmaceutics13111863
  15. Oliveira, Filipa D.; Castanho, Miguel A. R. B.; Neves, Vera. "Exosomes and Brain Metastases: A Review on Their Role and Potential Applications". International Journal of Molecular Sciences 22 19 (2021): 10899. http://dx.doi.org/10.3390/ijms221910899.
    10.3390/ijms221910899
  16. Sandra Isabel Aguiar; Joana N. R. Dias; Ana André; Marta Lisete Silva; Diana Martins; Belmira Carrapiço; Miguel Castanho; et al. "Highly Specific Blood-Brain Barrier Transmigrating Single-Domain Antibodies Selected by an In Vivo Phage Display Screening". Pharmaceutics 13 10 (2021): 1598. https://www.mdpi.com/1999-4923/13/10/1598.
    10.3390/pharmaceutics13101598
  17. Mink, Christian; Strandberg, Erik; Wadhwani, Parvesh; Melo, Manuel N.; Reichert, Johannes; Wacker, Irene; Castanho, Miguel A. R. B.; Ulrich, Anne S.. "Overlapping Properties of the Short Membrane-Active Peptide BP100 With (i) Polycationic TAT and (ii) a-helical Magainin Family Peptides". Frontiers in Cellular and Infection Microbiology 11 (2021): http://dx.doi.org/10.3389/fcimb.2021.609542.
    10.3389/fcimb.2021.609542
  18. Gallo, Maria; Moreno, Estefanía; Defaus, Sira; Ortega-Alvaro, Antonio; Gonzalez, Angel; Robledo, Patricia; Cavaco, Marco; et al. "Orally Active Peptide Vector Allows Using Cannabis to Fight Pain While Avoiding Side Effects". Journal of Medicinal Chemistry 64 10 (2021): 6937-6948. http://dx.doi.org/10.1021/acs.jmedchem.1c00484.
    10.1021/acs.jmedchem.1c00484
  19. Cavaco, Marco; Valle, Javier; Flores, Isabel; Andreu, David; A. R. B. Castanho, Miguel. "Estimating peptide half-life in serum from tunable, sequence-related physicochemical properties". Clinical and Translational Science (2021): http://dx.doi.org/10.1111/cts.12985.
    10.1111/cts.12985
  20. Marco Cavaco; David Andreu; Miguel A. R. B. Castanho. "The Challenge of Peptide Proteolytic Stability Studies: Scarce Data, Difficult Readability, and the Need for Harmonization". Angewandte Chemie (2021): https://doi.org/10.1002/ange.202006372.
    10.1002/ange.202006372
  21. Cadima-Couto, Iris; Tauzin, Alexandra; Freire, João M.; Figueira, Tiago N.; Silva, Rúben D. M.; Pérez-Peinado, Clara; Cunha-Santos, Catarina; et al. "Anti-HIV-1 Activity of pepRF1, a Proteolysis-Resistant CXCR4 Antagonist Derived from Dengue Virus Capsid Protein". ACS Infectious Diseases 7 1 (2020): 6-22. http://dx.doi.org/10.1021/acsinfecdis.9b00507.
    10.1021/acsinfecdis.9b00507
  22. Ana Silva Herdade; Iara Mota Silva; Ângelo Calado; Carlota Saldanha; Ngan-Ha Nguyen; Isabella Hou; Miguel Castanho; Sayon Roy. "Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy". Biomolecules (2020): https://doi.org/10.3390/biom10111583.
    10.3390/biom10111583
  23. Lousa, Diana; Pinto, Antónia R. T.; Campos, Sara R. R.; Baptista, António M.; Veiga, Ana S.; Castanho, Miguel A. R. B.; Soares, Cláudio M.. "Effect of pH on the influenza fusion peptide properties unveiled by constant-pH molecular dynamics simulations combined with experiment". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-77040-y.
    10.1038/s41598-020-77040-y
  24. de Almeida Fuzeta, Miguel; Bernardes, Nuno; Oliveira, Filipa D.; Costa, Ana Catarina; Fernandes-Platzgummer, Ana; Farinha, José Paulo; Rodrigues, Carlos A. V.; et al. "Scalable Production of Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Under Serum-/Xeno-Free Conditions in a Microcarrier-Based Bioreactor Culture System". Frontiers in Cell and Developmental Biology 8 (2020): http://dx.doi.org/10.3389/fcell.2020.553444.
    10.3389/fcell.2020.553444
  25. Cavaco, Marco; Pérez-Peinado, Clara; Valle, Javier; Silva, Rúben D. M.; Correia, João D. G.; Andreu, David; Castanho, Miguel A. R. B.; Neves, Vera. "To What Extent Do Fluorophores Bias the Biological Activity of Peptides? A Practical Approach Using Membrane-Active Peptides as Models". Frontiers in Bioengineering and Biotechnology 8 (2020): http://dx.doi.org/10.3389/fbioe.2020.552035.
    10.3389/fbioe.2020.552035
  26. Skalska, Julia; Andrade, Vitor M.; Cena, Gabrielle L.; Harvey, Peta J.; Gaspar, Diana; Mello, Érica O.; Henriques, Sónia T.; et al. "Synthesis, Structure, and Activity of the Antifungal Plant Defensin PvD1". Journal of Medicinal Chemistry 63 17 (2020): 9391-9402. http://dx.doi.org/10.1021/acs.jmedchem.0c00543.
    10.1021/acs.jmedchem.0c00543
  27. Belo, Rita F.; Martins, Margarida L. F.; Shvachiy, Liana; Costa-Coelho, Tiago; de Almeida-Borlido, Carolina; Fonseca-Gomes, João; Neves, Vera; et al. "The Neuroprotective Action of Amidated-Kyotorphin on Amyloid ß Peptide-Induced Alzheimer’s Disease Pathophysiology". Frontiers in Pharmacology 11 (2020): http://dx.doi.org/10.3389/fphar.2020.00985.
    10.3389/fphar.2020.00985
  28. Sousa, David Cordeiro; Leal, Inês; Moreira, Susana; do Vale, Sónia; Silva-Herdade, Ana S.; Aguiar, Patrício; Dionísio, Patrícia; et al. "Retinal Vascular Reactivity in Type 1 Diabetes Patients Without Retinopathy Using Optical Coherence Tomography Angiography". Investigative Opthalmology & Visual Science 61 6 (2020): 49. http://dx.doi.org/10.1167/iovs.61.6.49.
    10.1167/iovs.61.6.49
  29. Marco Cavaco; Javier Valle; Ruben da Silva; João D.G. Correia; Miguel A. R. B Castanho; David Andreu; Vera Neves. "DPepH3, an Improved Peptide Shuttle for Receptor-independent Transport Across the Blood-Brain Barrier". Current Pharmaceutical Design 26 13 (2020): 1495-1506. https://doi.org/10.2174/1381612826666200213094556.
    10.2174/1381612826666200213094556
  30. Aroeira, Rita I.; A.R.B. Castanho, Miguel. "Can citation metrics predict the true impact of scientific papers?". The FEBS Journal (2020): http://dx.doi.org/10.1111/febs.15255.
    10.1111/febs.15255
  31. Conceição, Katia; de Cena, Gabrielle L.; da Silva, Verônica A.; de Oliveira Neto, Xisto Antonio; de Andrade, Vitor Martins; Tada, Dayane Batista; Richardson, Michael; et al. "Design of bioactive peptides derived from CART sequence isolated from the toadfish Thalassophryne nattereri". 3 Biotech 10 4 (2020): http://dx.doi.org/10.1007/s13205-020-2151-4.
    10.1007/s13205-020-2151-4
  32. Castanho, Miguel. "Antibodies for the Treatment of Brain Metastases, a Dream or a Reality?". Pharmaceutics (2020): http://dx.doi.org/10.3390/pharmaceutics12010062.
    10.3390/pharmaceutics12010062
  33. Aroeira, Rita I.; Castanho, Miguel A. R. B.. "Pharmaceutical Innovations: The Grand Challenges Ahead". Frontiers in Medical Technology 1 (2020): http://dx.doi.org/10.3389/fmedt.2019.00003.
    Acesso aberto • Publicado • 10.3389/fmedt.2019.00003
  34. Tiago N. Figueira; Marco M. Domingues; Françoise Illien; Iris Cadima-Couto; Toni Todorovski; David Andreu; Sandrine Sagan; et al. "Enfuvirtide-Protoporphyrin IX Dual-Loaded Liposomes: In Vitro Evidence of Synergy against HIV-1 Entry into Cells". ACS Infectious Diseases (2019): https://doi.org/10.1021/acsinfecdis.9b00285.
    Acesso aberto • Aceite para publicação • 10.1021/acsinfecdis.9b00285
  35. Martins de Andrade, Vitor; Bardají, Eduard; Heras, Montserrat; Ramu, Vasanthakumar G.; Junqueira, Juliana Campos; Diane dos Santos, Jéssica; Castanho, Miguel A.R.B.; Conceição, Katia. "Antifungal and anti-biofilm activity of designed derivatives from kyotorphin". Fungal Biology (2019): http://dx.doi.org/10.1016/j.funbio.2019.12.002.
    10.1016/j.funbio.2019.12.002
  36. Grégoire J.-B. Philippe; Diana Gaspar; Caibin Sheng; Yen-Hua Huang; Aurélie H. Benfield; Nicholas D. Condon; Joachim Weidmann; et al. "Cell Membrane Composition Drives Selectivity and Toxicity of Designed Cyclic Helix–Loop–Helix Peptides with Cell Penetrating and Tumor Suppressor Properties". ACS Chemical Biology (2019): https://doi.org/10.1021/acschembio.9b00593.
    10.1021/acschembio.9b00593
  37. Santa-Maria, Ana R.; Walter, Fruzsina R.; Valkai, Sándor; Brás, Ana Rita; Mészáros, Mária; Kincses, András; Klepe, Adrián; et al. "Lidocaine turns the surface charge of biological membranes more positive and changes the permeability of blood-brain barrier culture models". Biochimica et Biophysica Acta (BBA) - Biomembranes 1861 9 (2019): 1579-1591. http://dx.doi.org/10.1016/j.bbamem.2019.07.008.
    10.1016/j.bbamem.2019.07.008
  38. Pérez-Peinado, Clara; Dias, Susana A.; Mendonça, Diogo A.; Castanho, Miguel A.R.B.; Veiga, Ana S.; Andreu, David. "Structural determinants conferring unusual long life in human serum to rattlesnake-derived antimicrobial peptide Ctn[15-34]". Journal of Peptide Science 25 8 (2019): http://dx.doi.org/10.1002/psc.3195.
    10.1002/psc.3195
  39. Thais H. Costa Petrin; Valmir Fadel; Danubia B. Martins; Susana A. Dias; Ana Cruz; Luciana Marciano Sergio; Manoel Arcisio-Miranda; Miguel A. R. B. Castanho; Marcia P. dos Santos Cabrera. "Synthesis and Characterization of Peptide–Chitosan Conjugates (PepChis) with Lipid Bilayer Affinity and Antibacterial Activity". Biomacromolecules (2019): https://doi.org/10.1021/acs.biomac.9b00501.
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  43. Julia Skalska; Filipa D. Oliveira; Tiago N. Figueira; Érica O. Mello; Valdirene M. Gomes; Grant McNaughton-Smith; Miguel A. R. B. Castanho; Diana Gaspar. "Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes". Nanoscale (2019): https://doi.org/10.1039/C9NR07843F.
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  44. Tiago N. Figueira; Diogo A. Mendonça; Diana Gaspar; Manuel N. Melo; Anne Moscona; Matteo Porotto; Miguel A. R. B. Castanho; Ana Salomé Veiga. "Structure–Stability–Function Mechanistic Links in the Anti-Measles Virus Action of Tocopherol-Derivatized Peptide Nanoparticles". ACS Nano 12 10 (2018): 9855-9865. https://doi.org/10.1021/acsnano.8b01422.
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  45. Avram, Speranta; Milac, Adina-Luminita; Borcan, Livia-Cristina; Mihailescu, Dan; Borcan, Florin; Castanho, Miguel. "Designing of Artificial Peptides for an Improved Antiviral Activity". Current Proteomics 15 4 (2018): 258-266. http://dx.doi.org/10.2174/1570164615666180409151111.
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  48. Neves-Coelho, Sara; Eleutério, Rute; Enguita, Francisco; Neves, Vera; Castanho, Miguel. "A New Noncanonical Anionic Peptide That Translocates a Cellular Blood–Brain Barrier Model". Molecules 22 10 (2017): 1753. http://dx.doi.org/10.3390/molecules22101753.
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  49. Juliana Perazzo; Carla Lima; Montserrat Heras; Eduard Bardají; Mônica Lopes-Ferreira; Miguel Castanho. "Neuropeptide Kyotorphin Impacts on Lipopolysaccharide-Induced Glucocorticoid-Mediated Inflammatory Response. A Molecular Link to Nociception, Neuroprotection, and Anti-Inflammatory Action". ACS Chemical Neuroscience (2017): https://doi.org/10.1021%2Facschemneuro.7b00007.
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  50. Figueira, Tiago N.; Freire, João M.; Cunha-Santos, Catarina; Heras, Montserrat; Gonçalves, João; Moscona, Anne; Porotto, Matteo; Salomé Veiga, Ana; Castanho, Miguel A. R. B.. "Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance". Scientific Reports 7 1 (2017): http://dx.doi.org/10.1038/srep45647.
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  53. Freire, João Miguel; Rego de Figueiredo, Inês; Valle, Javier; Veiga, Ana Salomé; Andreu, David; Enguita, Francisco J.; Castanho, Miguel A.R.B.. "siRNA-cell-penetrating peptides complexes as a combinatorial therapy against chronic myeloid leukemia using BV173 cell line as model". Journal of Controlled Release 245 (2017): 127-136. http://dx.doi.org/10.1016/j.jconrel.2016.11.027.
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  56. Tiago N. Figueira; Filipa D. Oliveira; Inês Almeida; Érica O. Mello; Valdirene M. Gomes; Miguel A. R. B. Castanho; Diana Gaspar; et al. "Challenging metastatic breast cancer with the natural defensin PvD1". Nanoscale 9 43 (2017): 16887-16899. https://doi.org/10.1039/C7NR05872A.
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  57. Santos, Raquel; Gomes, Diana; Macedo, Hermes; Barros, Diogo; Tibério, Catarina; Veiga, Ana Salomé; Tavares, Luís; Castanho, Miguel; Oliveira, Manuela. "Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates". Journal of Medical Microbiology 65 10 (2016): 1092-1099. http://dx.doi.org/10.1099/jmm.0.000329.
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  59. Sá Santos, Sónia; Santos, Sara M.; Pinto, Antónia R. T.; Ramu, Vasanthakumar G.; Heras, Montserrat; Bardaji, Eduard; Tavares, Isaura; Castanho, Miguel A. R. B.. "Amidated and Ibuprofen-Conjugated Kyotorphins Promote Neuronal Rescue and Memory Recovery in Cerebral Hypoperfusion Dementia Model". Frontiers in Aging Neuroscience 8 (2016): http://dx.doi.org/10.3389/fnagi.2016.00001.
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  60. Freire, João Miguel; Almeida Dias, Susana; Flores, Luís; Veiga, Ana Salomé; Castanho, Miguel A.R.B.; Freire, J.M.; Dias, S.A.; et al. "Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides". Bioinformatics 31 14 (2015): 2252-2256. http://dx.doi.org/10.1093/bioinformatics/btv131.
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  63. Serrano, Isa D.; Ramu, Vasanthakumar G.; Pinto, Antónia R. T.; Freire, João M.; Tavares, Isaura; Heras, Montserrat; Bardaji, Eduard R.; Castanho, Miguel A. R. B.. "Correlation between membrane translocation and analgesic efficacy in kyotorphin derivatives". Biopolymers 104 1 (2015): 1-10. http://dx.doi.org/10.1002/bip.22580.
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  102. Castanho, M.A.R.B.; Santos, N.C.; Loura, L.M.S.. "Separating the turbidity spectra of vesicles from the absorption spectra of membrane probes and other chromophores". European Biophysics Journal 26 3 (1997): 253-259. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030823194&partnerID=MN8TOARS.
    10.1007/s002490050078
  103. Castanho, M.A.; Brown, W.; Prieto, M.J.. "Rod-like cholesterol micelles in aqueous solution studied using polarized and depolarized dynamic light scattering". Biophysical Journal 63 6 (1992): 1455-1461. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027078034&partnerID=MN8TOARS.
    10.1016/S0006-3495(92)81733-2
Artigo em revista (magazine)
  1. Castanho, Miguel. "Why we can't resist sugar - what science says?", Visão Saúde, 2019, http://www.pt.cision.com/cp2013/ClippingDetails.aspx?id=8a74f51c-b7f0-4e1a-b37f-417c2ec160f9&analises=1.
Livro
  1. Da Poian, Andrea T.; Castanho, Miguel A. R. B.. Integrative Human Biochemistry. Springer International Publishing. 2021.
    10.1007/978-3-030-48740-9
Poster em conferência
  1. Castanho, Miguel. "“Blood-based biomarker for Alzheimer’s disease.” Santos I., Neves V., Castanho M. iMed conference® 7.0, Lisbon, 17-20th September 2015.". 2015.
Recurso online
  1. Castanho, Miguel. "What's hidden in viral proteins? Lessons from Dengue virus capsid", - CEDOC Seminars. 2019. https://www.youtube.com/watch?v=rqkwU60p2l8.

Propriedade Intelectual

Patente
  1. 2022. "ANTI-INFECTIVE CONJUGATE FOR USE IN THE TREATMENT OF ZIKA VIRUS INFECTION". Alemanha.
    Pendente
  2. A.R.B. Castanho, Miguel. 2021. "Antibody Molecules and Peptide Delivery Systems for Use in Alzheimer's Disease and Related Disorders". Estados Unidos.
    Concedida/Emitida
  3. Castanho, Miguel. 2020. "Antibody molecules and peptide delivery systems for use in Alzheimer's disease or related disorders". Estados Unidos.
  4. Castanho, Miguel. 2020. "C-X-C motif chemokine receptor 4 antagonist".
    Pendente
  5. Castanho, Miguel. 2019. "Peptide compounds for reducing side effects of CB1 receptor agonists".
  6. Castanho, Miguel. 2016. "Antibody Molecules And Peptide Delivery Systems For Use In Alzheimer’s Disease And Related Disorders".

Outros

Outra produção
  1. "Targeting brain metastases with a peptide conjugate". Cavaco M, Pérez-Peinado C, Gaspar D, Andreu D, Castanho MARB, Neves V. FEBS OPEN BIO 9: 372.. 2019. Castanho, Miguel.
  2. "Linking Structure, Stability and Function in the Anti-Measles Virus Action of Tocopherol-Derivatized Peptide Nanoparticles". Figueira TN, Mendonça DA, Gaspar D, Melo MN, Moscona A, Porotto M, Castanho MARB, Veiga AS. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 48: S158-S158.. 2019. Castanho, Miguel.
  3. "Effect of pH on the influenza fusion peptide structure and activity: A constant-pH molecular dynamics approach". Lousa D, Pinto ART, Campos SRR, Baptista AM, Veiga AS, Castanho MARB, Soares CM. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 48: S47-S47.. 2019. Castanho, Miguel.
  4. "The efficacy of designed anti-measles virus peptides depends on the stability of self-assembled clusters". Mendonça DA, Figueira TF, Melo M, Harder O, Niewiesk S, Moscona A, Porotto M, Castanho MARB, Veiga AS. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 48: S81-S81.. 2019. Castanho, Miguel.
  5. "Anticancer activity of a novel cell -penetrating peptide, vCPP2319, and its interaction with breast cancer cells' derived exosomes". Oliveira FD, Figueira TN, Napoleao P, Andreu D, Castanho MARB, Gaspar D. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 48: S116-S116.. 2019. Castanho, Miguel.
  6. "Insights into the mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms". Pinto SN, Dias SA, Cruz AF, Mil-Homens D, Fernandes F, Valle J, Andreu D, Prieto M, Castanho MARB, Coutinho A, Veiga AS. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 48: S117-S117.. 2019. Castanho, Miguel.
  7. "Effects of Diabetes on Microcirculation in Non-Ocular Tissues: Insights into Hemodynamic Alterations in Diabetic Retinopathy". Silva-Herdade AS, Saldanha C, Ha N, Hou I, Castanho MARB, Roy S. Journal of Vascular Research 56, 101.. 2019. Castanho, Miguel.
  8. "Novel mechanism of action of natural plant defensin PvD1 involves a modulatory effect of CD63 and CD9 expression level in breast cancer cells derived exosomes". Skalska J, Oliveira FD, Figueira TN, Mello EO, Gomes VM, Castanho MARB, Gaspar D. FEBS OPEN BIO 9: 325.. 2019. Castanho, Miguel.
  9. "Dengue Virus Capsid Protein Interacts Specifically with Very Low-Density Lipoproteins". Faustino, André F.; Carvalho, Filomena A.; Martins, Ivo C.; Castanho, Miguel A.R. B.; Mohana-Borges, Ronaldo; Almeida, Fabio C.L.; Da Poian, Andrea T.; Santos, Nuno C.. Biophysical Journal 106 (2): 388a. http://dx.doi.org/10.1016/j.bpj.2013.11.2194.. 2014. Castanho, Miguel.
  10. "The Dengue Virus Capsid Protein Inhibitor Peptide Pep14-23 becomes Alpha-Helical upon Binding to Negative Lipids". Faustino, André F.; Artilheiro, Vanessa; Castanho, Miguel A.R.B.; Almeida, Fábio;Da Poian, Andrea T.; Santos, Nuno C.; Martins, Ivo C.. Biophysical Journal 104 (2): 536a. http://dx.doi.org/10.1016/j.bpj.2012.11.2970.. 2013. Castanho, Miguel.
  11. "Dengue Virus Capsid Protein Binding to Lipid Droplets and its Inhibition.towards a New Drug Target". Carvalho, Filomena A.; Martins, Ivo C.; Carneiro, Fabiana A.; Assunção-Miranda,Iranaia; Faustino, André F.; Castanho, Miguel; Mohana-Borges, Ronaldo; et al. Biophysical Journal 104 (2): 415a. http://dx.doi.org/10.1016/j.bpj.2012.11.2313.. 2013. Castanho, Miguel.
  12. "Decoding Distinct Membrane Interactions of HIV-1 Inhibitors Enfuvirtideand T-1249 using Atomic Force Microscopy Combined with FluorescenceMethodologies". Franquelim, Henri G.; Veiga, A. Salomé; Santos, Nuno C.; Castanho, Miguel A.. Biophysical Journal 102 (3): 490a. http://dx.doi.org/10.1016/j.bpj.2011.11.2685.. 2012. Castanho, Miguel.
  13. "Short Stories - onAntimicrobial Peptides that are Too Short to Span the Membrane". Ulrich, Anne S.; Heidenreich, Nico; Wadhwani, Parvesh; Reichert, Johannes;Strandberg, Erik; Misievicz, Julia; Afonin, Sergii; et al. Biophysical Journal 102 (3): 616a. http://dx.doi.org/10.1016/j.bpj.2011.11.3358.. 2012. Castanho, Miguel.
  14. "Relating Molecular-Level Events with Bacterial Killing by AntimicrobialPeptides". Melo, Manuel N.; Ferre, Rafael; Alves, Carla; Castanho, Miguel A.R.B.. Biophysical Journal 102 (3): 91a-91a. http://dx.doi.org/10.1016/j.bpj.2011.11.518.. 2012. Castanho, Miguel.
  15. "Conjugation of Cholesterol to HIV-1 Fusion Inhibitor C34 SynergisticallyPotentiates Peptide-Membrane Interaction". Matos, Pedro M.; Hollmann, Axel; Castanho, Miguel A.; Santos, Nuno C. Biophysical Journal 102 (3): 490a-490a. http://dx.doi.org/10.1016/j.bpj.2011.11.2684.. 2012. Castanho, Miguel.
  16. "rBPI21 Causes(HEMI)Fusion and Leakage of Membranes and Promotes Massive Lipopolysaccharide Aggregation". Domingues, Marco; Castanho, Miguel; Santos, Nuno C. Biophysical Journal 100 (3): 495a-495a. http://dx.doi.org/10.1016/j.bpj.2010.12.2901.. 2011. Castanho, Miguel.
  17. "Characterization of the Interaction of the Dengue Virus Capsid Protein withLipid Droplets". Martins, Ivo C.; Carneiro, Fabiana A.; Mohana-Borges, Ronaldo; Faustino, André;Pereira, Renata M.; Carvalho, Filomena A.; Castanho, Miguel A.; et al.. Biophysical Journal 100 (3): 403a-404a. http://dx.doi.org/10.1016/j.bpj.2010.12.2396.. 2011. Castanho, Miguel.
  18. "Human Erythrocytes And Mononuclear Leukocytes Are Capable Of Concentrating HIV-1 Fusion Inhibitor Peptides In Their Membranes". Matos, Pedro M.; Castanho, Miguel A.R.B.; Santos, Nuno C.. Biophysical Journal 96 (3):157a-157a. http://dx.doi.org/10.1016/j.bpj.2008.12.715.. 2009. Castanho, Miguel.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2021/06/14 'Pursuing Innovative Peptide Drugs with Biophysical Approaches: Why, What, What for?' - Opening Plenary Lecture. 7th International Iberian Biophysics Congress
Iberian Biophysical Societies (Coimbra, Portugal)
2021/04/09 “Diffusion-lysis coupling of antimicrobial peptides in biofilms of S. aureus.” - Keynote speaker M Castanho, keynote speaker.
University Graz (Graz, Áustria)

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação) 		Instituição / Organização
2020/09/05 - Atual “Nanomedicine – tiny tools to beat the giant cancer challenge.” Session of ESOF2020 – Euroscience Open Forum (European Commission), Speaker and session chairman. Turin, Italy. (2020/09/05)
Congresso (Coorganizador)
 Commissione Europea Rappresentanza in Italia, Itália

Arbitragem científica em conferência

Nome da conferência Local da conferência
2022/07/09 - Atual “The Biochemistry Global Summit 2022” FEBS, IUBMB, and PABMB joint congress - Chairman of the FEBS, IUBMB, and PABMB.

Entrevista (jornal / revista)

Descrição da atividade Jornal / Forum
2019 Interview in Canal Saúde+: Raio X.

Entrevista / Programa (rádio / tv)

Programa Tema
2020/02/10 - Atual "A synthesis of what we know about viruses". - "Antena 2 Ciência"
2019/12/03 - Atual INVESTIGAÇÃO ESTUDA MÉTODOS PARA COMBATER VÍRUS DA ZICA NO CÉREBRO
2019/10/14 - Atual What we already know about viruses and what we don't know yet
2019/10/07 - Atual Fighting viruses that attack the brain, a project by biochemist Miguel Castanho that received a European fund of 4.2 million euros
2019/03/05 - Atual HIV. Advances are positive but it is "premature" to speak in healing
Distinções

Prémio

2023 Biophysical Society, USA; Ambassador
2022 Biochemical Society UK; Ambassador
2022 EIC; Ambassador
2019 Scientific Award Universidade de Lisboa / Caixa Geral de Depósitos 2018 in the scientific area of Biomedical Sciences.
2019 Pedagogical Merit Award of Prof. Doutor J. Gomes-Pedro
Universidade de Lisboa Faculdade de Medicina, Portugal

Outra distinção

2020 Personality of the year (for outreach and science communication)
2020 Ranked among the top 1.1% of most cited authors in Biophysics according to a science-wide author database of standardized citation indicators (PLoS Biol 18: e3000918; 2020. doi.org/10.1371/journal.pbio.3000918)
2019 Appointed Chief Editor of Pharmaceutical Innovation, a speciality journal of Frontiers in Medical Technology, Springer Nature Publishing Group. (https://www.frontiersin.org/journals/medical-technology/sections/pharmaceutical-innovation)
2015 Grünenthal Dor Award, Clinical Research
Fundação Grünenthal, Portugal
2014 Leonidas Zervas award
European Peptide Society, Espanha
2012 International Local Embassador
Biochemical Society UK, Reino Unido
2012 Specially invited researcher on Medical Biochemistry (Postgraduation and Outreach Programs)
Universidade Federal do Rio de Janeiro, Brasil
2011 Biophytsicist in profile - Miguel Castanho
2010 I2P - Ideas to Products, European competition (2nd Best Technology)
2009 Grünenthal Dor Award
2005 Prof José Luis Champalimaud award
2004 Vicente de Seabra Medal
2004 Dr. José Luis Champalimaud Award
Direcção-Geral da Saúde, Portugal
1996 Programa Gulbenkian de Estímulo à Investigação, Área de Fotofísica e Fotoquímica.
Fundação Calouste Gulbenkian, Portugal