Fátima Alexandra Meira Machado (DA12-F831-7439)

Curriculum Vitae

Fátima Alexandra Meira Machado

Graduated in Applied Biology in 2011, Alexandra specialized in nanosystems for drug delivery in the Master's Degree in Biophysics and Bionanosystems, both in University of Minho. Over the following two and a half years, Alexandra collaborated in two research projects, one at the University of Porto (INEB), and the other at the University of Minho (CEB). With a Ph. D. in Biomedical Engineering (CEB, 2023), Alexandra has published 9 articles, 1 book chapter, and 1 patent in the biomedical field. Alexandra has experience in: 1) production and physicochemical characterization of biomaterials; 2) bionanoconjugates and biosensors; 3) nanoencapsulation of antimicrobial bioactives in micellar, polymeric, liposomal and hydrogel structures; 4) microbiology, with a focus on antimicrobial assays; and 5) comprehensive characterization of biocompatibility, including cytotoxicity, intracellular localization and biodistribution. Alexandra has currently two years of post-doctoral experience (fev 2023 - now) in the field of ¿¿agriculture at InnovPlantProtect, particularly in the development of nano and microencapsulation systems for bioprotectants and/or biostimulants, stabilization and shelf-life extension of agricultural formulations. Alexandra extended her experience in microbiology to growth inhibition tests of pathogenic bacteria and fungi of relevance in agriculture. Alexandra has 1 published patent in this area (WO2024/018434 A1). As a Ph. D. researcher, Alexandra is currently working within the scope of the Blue Bioeconomy Pact Agenda (Vertical Algae, subproject 6).

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Identification

Personal identification

Full name: Fátima Alexandra Meira Machado

Citation names

Machado, Alexandra

Author identifiers

Ciência ID: DA12-F831-7439
ORCID iD: 0000-0002-9347-2407

Addresses

InnovPlantProtect CoLab, Estrada de Gil Vaz, 7350-478, Elvas, Elvas, Portugal (Professional)

Knowledge fields

Engineering and Technology - Nano-technology
Natural sciences - Biological Sciences - Biochemistry
Medical and Health Sciences - Medical Biotechnology - Biomaterials
Engineering and Technology - Medical Engineering

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)

Education

	Degree	Classification
2023/02/24 Concluded	Engenharia Biomédica (Doutoramento) Major in Sem especialidade Universidade do Minho, Portugal "Dextrin: a platform for the development of Drug Delivery Systems." (THESIS/DISSERTATION)
2014 Concluded	Biofísica e Bionanossistemas (Mestrado) Major in Sem especialização Universidade do Minho, Portugal "Development of a Liposomal Formulation for Peptide Delivery to Serve as Vaccine against Chronic Myeloid Leukemia" (THESIS/DISSERTATION)
2007/09/01 - 2011/07/31 Concluded	Biologia Aplicada (Licenciatura) Universidade do Minho, Portugal

Affiliation

Science

	Category Host institution	Employer
2023/03/01 - Current	Researcher (Research)	InnovPlantProtect, Portugal
	InnovPlantProtect, Portugal
2016/10/01 - 2018/01/31	Researcher (Research)	Universidade do Minho Centro de Engenharia Biológica, Portugal
	Universidade do Minho Centro de Engenharia Biológica, Portugal
2014/09/01 - 2015/08/31	Researcher (Research)	Instituto Nacional de Engenharia Biomédica, Portugal
	Instituto Nacional de Engenharia Biomédica, Portugal

Projects

Grant

	Designation	Funders
2018/01 - 2022/06	Dextrin: a Platform for Drug Delivery Systems SFRH/BD132000/2017 PhD Student Fellow Universidade do Minho Centro de Engenharia Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2014/09 - 2015/08	Development of antiretroviral-loaded nanoparticles-in-vaginal film systems for preventing HIV transmission VIH/SAU/0021/2011 Research Fellow Instituto Nacional de Engenharia Biomédica, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2023/03 - 2025/12	Blue Bioeconomy Innovation Pact - Algae Vertical - SP6 C644915664-00000026 Post-doc InnovPlantProtect, Portugal
2016 - 2019	iBONE therapies - Terapias inovadoras para a regeneração óssea 003262 Research Fellow Universidade do Minho Centro de Engenharia Biológica, Portugal	Agência Nacional de Inovação SA Concluded

Outputs

Publications

Book	Machado, A.; Das Neves, J.. Tissue-based in vitro and ex vivo models for vaginal permeability studies. 2015. 10.1016/B978-0-08-100094-6.00016-X
Book chapter	Machado, Alexandra; das Neves, José. "Tissue-based models for vaginal permeability studies". In Concepts and Models for Drug Permeability Studies: Cell and Tissue Based In Vitro Culture Models. Woodhead Publishing, 2024. In press
Conference poster	Machado, Alexandra; Gama, Miguel; José Alberto Martins. "Therapeutic partnership of cathelicidin-derived LLKKK18 loaded into vitamin d-dextrin micelles tailored for bone infections.". Paper presented in 16th European Symposium on Controlled Drug Delivery, 2022. Machado, Alexandra; José Alberto Martins; Pereira, José Eduardo; Gama, Miguel. "Dextrin: a platform for the development of Drug Delivery Systems.". Paper presented in 13th International Chemical and Biological Engineering Conference 2018 Conference - Aveiro, Portugal., 2018. Rodrigues, Ana Cristina; Cardoso, Beatriz; Machado, Alexandra; Queirós, Eugénia; Lima, Sofia Costa; Reis, Salette; Silva, João Pedro; Professor Doutor Francisco Miguel Portela da Gama. "Development of bacterial cellulose wound dressings with controlled delivery of vitamin D3". Paper presented in CEB Annual Meeting 2017, 2017. Rodrigues, Ana Cristina; Beatriz Cardoso; Machado, Alexandra; Eugénia Queirós; Sofia Costa Lima; Salette Reis; João Pedro Silva; Gama, Miguel. "Development of bacterial cellulose wound dressing with controlled delivery of vitamin D3.". Paper presented in CEB annual meeting - Braga, Portugal., 2017. das Neves, José; Cunha-Reis, Cassilda; Barreiros, Luisa; Machado, Alexandra; Araújo, Francisca; Nunes, Rute; Sarmento, Bruno. "Pharmacokinetics and safety of vaginal tenofovir/efavirenz-loaded nanoparticles-in-film in mice". Paper presented in HIV Research for Prevention 2016: AIDS Vaccine, Microbicide and ARV-based Prevention Science (HIVR4P), Chicago, IL, USA, 2016., 2016. Machado, Alexandra; Cunha-Reis, Cassilda; das Neves, José; Sarmento, Bruno. "Antiretroviral-loaded nanoparticle-in-vaginal film systems for preventing HIV transmission: safety and toxicity.". Paper presented in 51st Congress of the European Societies of Toxicology Bridging Sciences for Safety - Porto, Portugal., 2015.
Journal article	Machado, Alexandra; Gama, Miguel; Martins, José Alberto. "Development of a dextrin–vitamin D3 micelle nanocarrier for the antimicrobial peptide LLKKK18 as a potential therapeutic agent for bone infections". Journal of Materials Chemistry B (2024): http://dx.doi.org/10.1039/d4tb00903g. 10.1039/d4tb00903g Alexandra Machado; Isabel Pereira; José Eduardo Pereira; Luís Maltez; Ana Brandão; Rui Alvites; Ana Catarina Sousa; et al. "Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair". Materialia (2023): https://doi.org/10.1016/j.mtla.2023.101859. 10.1016/j.mtla.2023.101859 Alexandra Machado; Isabel Pereira; Filomena Costa; Ana Brandão; José Eduardo Pereira; Ana Colette Maurício; José Domingos Santos; et al. "Randomized clinical study of injectable dextrin-based hydrogel as a carrier of a synthetic bone substitute". Clinical Oral Investigations (2023): http://dx.doi.org/10.1007/s00784-023-04868-9. 10.1007/s00784-023-04868-9 Alexandra Machado. "Injectable hydrogel as a carrier of vancomycin and a cathelicidin-derived peptide for osteomyelitis treatment". Journal of Biomedical Materials Research Part A (2022): http://dx.doi.org/10.1002/jbm.a.37432. 10.1002/jbm.a.37432 Cristelo, Cecília; Machado, Alexandra; Gama, Miguel; Sarmento, Bruno. "The roles of vitamin D and cathelicidin in type 1 diabetes susceptibility". Endocrine Connections (2021): https://pubmed.ncbi.nlm.nih.gov/33263562/. 10.1530/EC-20-0484 Cunha-Reis, C.; Machado, A.; Barreiros, L.; Araújo, F.; Nunes, R.; Seabra, V.; Ferreira, D.; et al. "Nanoparticles-in-film for the combined vaginal delivery of anti-HIV microbicide drugs". Journal of Controlled Release 243 (2016): 43-53. http://www.scopus.com/inward/record.url?eid=2-s2.0-84990849820&partnerID=MN8TOARS. 10.1016/j.jconrel.2016.09.020 Machado, A.; Cunha-Reis, C.; Araújo, F.; Nunes, R.; Seabra, V.; Ferreira, D.; das Neves, J.; Sarmento, B.. "Development and in vivo safety assessment of tenofovir-loaded nanoparticles-in-film as a novel vaginal microbicide delivery system". Acta Biomaterialia 44 (2016): 332-340. http://www.scopus.com/inward/record.url?eid=2-s2.0-84992121833&partnerID=MN8TOARS. 10.1016/j.actbio.2016.08.018 González-Delgado, J.A.; Castro, P.M.; Machado, A.; Araújo, F.; Rodrigues, F.; Korsak, B.; Ferreira, M.; Tomé, J.P.C.; Sarmento, B.. "Hydrogels containing porphyrin-loaded nanoparticles for topical photodynamic applications". International Journal of Pharmaceutics 510 1 (2016): 221-231. http://www.scopus.com/inward/record.url?eid=2-s2.0-84976426703&partnerID=MN8TOARS. 10.1016/j.ijpharm.2016.06.037 das Neves, J.; Nunes, R.; Machado, A.; Sarmento, B.. "Polymer-based nanocarriers for vaginal drug delivery". Advanced Drug Delivery Reviews 92 (2015): 53-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-84943453074&partnerID=MN8TOARS. 10.1016/j.addr.2014.12.004

Intellectual property

Patent

Cristina Maria Lourenco da Cunha Correia de Azevedo; Margarida Basaloco-Fonseca; Tiago Miguel Marques Monteiro Amaro; Machado, Alexandra; David Alexander Learmonth; Pedro Carranca Nunes Rosa. 2024. "BIOLOGICAL PLANT PROTECTION AGENT, METHODS AND USES THEREOF".
Allowed
Sarmento, Bruno; das Neves, José; Machado, Alexandra; Cunha-Reis, Cassilda. 2017. "Composition for use in a method for prevention or treatment of human immunodeficiency virus infections".

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2019/09	Local delivery of LLKKK18 antimicrobial peptide released from oxidized dextrin hydrogel to treat osteomyelitis.	30th Conference of the European Society for Biomaterials (ESB) European Society for Biomaterials (Dresden, Germany)