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Mafalda Trovão dos Santos. Concluiu o Mestrado em Biotecnologia em 2019/10 pelo(a) Universidade Nova de Lisboa Departamento de Química e Licenciatura em Bioquímica em 2016/06/24 pelo(a) Universidade de Coimbra Departamento de Ciências da Vida. Publicou 5 artigos em revistas especializadas. Recebeu 1 prémio. Participa e/ou participou como Bolseiro de Mestrado em 1 projeto. Atua na área de Ciências Naturais com ênfase em Ciências Biológicas com ênfase em Bioquímica e Ciências da Engenharia e Tecnologias com ênfase em Biotecnologia Industrial com ênfase em Fermentação. No seu currículo Ciência Vitae os termos mais frequentes na contextualização da produção científica, tecnológica e artístico-cultural são: Thraustochytrids; Aurantiochytrium sp; docosahexaenoic acid (DHA); optimization; growth performance; Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química; microalgae; Tetraselmis sp; downstream; certified organic biomass; Chlorella vulgaris; photobioreactors; scale-up; media optimization; CO2 mitigation; Phaeodactylum tricornutum; biochemical composition; .
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Identification

Personal identification

Full name
Mafalda Trovão dos Santos
Gender
Female

Citation names

  • Trovão, Mafalda

Author identifiers

Ciência ID
D912-559A-7706
ORCID iD
0000-0002-9465-7681

Email addresses

  • mafalda.trovao@allmicroalgae.com (Professional)
  • mtd.santos@campus.fct.unl.pt (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry
  • Engineering and Technology - Industrial Biotechnology
  • Engineering and Technology - Industrial Biotechnology - Fermentation

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2)
French Elementary (A2) Elementary (A2) Elementary (A2) Elementary (A2)
Education
Degree Classification
2020/12/01 - 2024/11/30
Concluded
 Engenharia Biológica (Doutoramento)
Universidade Nova de Lisboa, Portugal
"Development of novel microalgal mutants with improved pigmentation and protein contents for industrial waste-based fermentation" (THESIS/DISSERTATION)
2017/09 - 2019/10
Concluded
 Biotecnologia (Mestrado)
Universidade Nova de Lisboa Departamento de Química, Portugal
"Heterotrophic growth of thraustochytrids strains – screening, optimization and scale-up " (THESIS/DISSERTATION)
 18,05
2013/09/16 - 2016/06/24
Concluded
 Bioquímica (Licenciatura)
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
"Final Project - Development of nanosystems to transport genetic material and drugs to use as antitumoral strategies" (THESIS/DISSERTATION)
 15,200
Affiliation

Science

Category
Host institution 		Employer
2025/01/01 - Current Postdoc (Research) Allmicroalgae, Portugal
2020/12 - 2024/12 Researcher (Research) Associação para a Inovação e Desenvolvimento da FCT, Portugal
Allmicroalgae, Portugal
(...)
2019/11 - 2020/11/01 Researcher (Research) Allmicroalgae, Portugal
2017/02 - 2017/11 Research Trainee (Research) Allmicroalgae, Portugal
2016/09 - 2016/12 Research Trainee (Research) Istituto di Biochimica delle Proteine Consiglio Nazionale delle Ricerche, Italy
Projects

Contract

Designation Funders
2022/01/01 - 2025/12/31 Zero Waste
101036388
10.3030/101036388
PhD Student Fellow
South East Technological University, Ireland

Aves Nobles y Derivados SL, Spain

F6S Network Limited, United Kingdom

Openbare Vlaamse Afvalstoffenmaatschappij, Belgium

Instituto Andaluz de Investigación y Formación Agraria Pesquera Alimentaria y de la Producción Ecológica, Spain

Novamont SpA, Italy

Instituto Tecnológico de Aragón ITAINNOVA, Spain

Instituto Tecnológico del Embalaje Transporte y Logistica, Spain

Univerza v Mariboru, Slovenia

Wageningen University, Netherlands

Universidade do Minho, Portugal
European Commission
Ongoing
2022 - 2024 MICROBOOST
PT-INNOVATION-0102
PhD Student Fellow
EEA Grants
2019/10/01 - 2023/09/30 ProFuture
862980
10.3030/862980
PhD Student Fellow
Allmicroalgae, Portugal
Concluded
2021 - 2023 Performalgae
ALG-01-0247-FEDER-069961
PhD Student Fellow
Concluded
2021 - 2023 Simba
210489259
PhD Student Fellow
EU Framework Programme for Research and Innovation Spreading Excellence and Widening Participation
2019/11 - 2020 AlgaValor
35234
Master Student Fellow
Concluded
Outputs

Publications

Conference abstract
  1. Trovão, Mafalda; Ana Barros; Adriana Machado; Joana Ribeiro; Bernardo Carvalho; Correia, Nádia; Humberto Pedroso; et al. "A non-GMO approach to isolate novel Chlorella vulgaris mutants with higher biomass productivities for food and feed applications". Paper presented in Algaeurope, 2021.
  2. Trovão, Mafalda; Ana Barros; Adriana Machado; Joana Ribeiro; Bernardo Carvalho; Correia, Nádia; Humberto Pedroso; et al. "Enhancement of Chlorella vulgaris biomass productivity through a non-GMO strain improvement approach for food and feed applications". Paper presented in Mikrobiokosmos, 2021.
  3. Correia, Nádia; Pereira, Hugo; Guerra, Inês; Cunha, Pedro; Joana Fonseca; Trovão, Mafalda; Adriana Machado; et al. "Bioprospecting, growth optimization and industrial cultivation of novel microalgae strains obtained from local ponds". Paper presented in Algaeurope, 2021.
Conference poster
  1. Correia, Nádia; Pereira, Hugo; Costa, Monya M; Guerra, Inês; Trovão, Mafalda; Ana Barros; Maria Soares; et al. "High-value biomass composition from heterotrophic and autotrophic cultivation of novel Chlorococcum amblystomatis". Paper presented in Algae Biomass Summit, 2022.
  2. Trovão, Mafalda; Schueler, Lisa; Ana Barros; Adriana Machado; Correia, Nádia; Humberto Pedroso; Gonçalo Espírito Santo; et al. "Isolation of novel Chlorella vulgaris mutants with improved organoleptic features for food applications: a non-GMO approach". Paper presented in Encontro Ciência 2022, 2022.
  3. Correia, Nádia; Pereira, Hugo; Guerra, Inês; Cunha, Pedro; Trovão, Mafalda; Adriana Machado; Ana Barros; et al. "Novel microalga Chlorococcum amblystomatis as an alternative resource of nutrients". Paper presented in Encontro Ciência 2022, 2022.
  4. Trovão, Mafalda; Ana Barros; Adriana Machado; Joana Ribeiro; Bernardo Carvalho; Correia, Nádia; Humberto Pedroso; et al. "Isolation of novel Chlorella vulgaris mutants with higher biomass productivities for food and feed applications: a non-GMO strain improvement approach". 2021.
  5. Correia, Nádia; Pereira, Hugo; Guerra, Inês; Cunha, Pedro; Joana Fonseca; Trovão, Mafalda; Adriana Machado; et al. "Microalgae: sustainable sources of nutrients". Paper presented in Microbiotec, 2021.
  6. Correia, Nádia; Pereira, Hugo; Guerra, Inês; Cunha, Pedro; Joana Fonseca; Trovão, Mafalda; Adriana Machado; et al. "Large scale production and biomass characterization of a novel Chlorococcum amblystomatis (Chlorophyta) strain". Paper presented in Algae Biomass Summit, 2021.
  7. Trovão, Mafalda. "In the path of DHA producers: lab-scale heterotrophic growth of Aurantiochytrium sp. 0043". Paper presented in Microbiotec'19, 2019.
Journal article
  1. Inês Guerra; Cátia Torres; Helena Cardoso; Hugo Pereira; Mafalda Trovão; Alexandre M.C. Rodrigues; João Varela. "Impact of seasons on industrial cultivation of Limnospira platensis (Spirulina): A year-round case study on biomass, phycocyanin, and protein productivity in Portugal". Algal Research (2025): http://dx.doi.org/10.1016/j.algal.2025.104335.
    10.1016/j.algal.2025.104335
  2. Mafalda Trovão; Miguel Cunha; Gonçalo Espírito Santo; Humberto Pedroso; Ana Reis; Ana Barros; Nádia Correia; et al. "Improving the heterotrophic media of three Chlorella vulgaris mutants toward optimal color, biomass and protein productivity". Scientific Reports (2025): http://dx.doi.org/10.1038/s41598-025-05913-1.
    10.1038/s41598-025-05913-1
  3. Mafalda Trovão; Ana Barros; Adriana Machado; Ana Reis; Humberto Pedroso; Gonçalo Espírito Santo; Nádia Correia; et al. "Heterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: Medium formulation and process scale-up". Journal of Environmental Chemical Engineering (2025): http://dx.doi.org/10.1016/j.jece.2025.115361.
    10.1016/j.jece.2025.115361
  4. Mafalda Trovão; Lisa Schüler; Humberto Pedroso; Ana Reis; Gonçalo Espírito Santo; Ana Barros; Nádia Correia; et al. "Isolation and Selection of Protein-Rich Mutants of Chlorella vulgaris by Fluorescence-Activated Cell Sorting with Enhanced Biostimulant Activity to Germinate Garden Cress Seeds". Plants (2024): https://doi.org/10.3390/plants13172441.
    10.3390/plants13172441
  5. Trovão, Mafalda; Cardoso, Lucas; Schüler, Lisa; Machado, Adriana; Santo, Gonçalo Espírito; Pedroso, Humberto; Reis, Ana; et al. "Oxyfluorfen: a novel metabolic inhibitor to select microalgal chlorophyll-deficient mutant strains for nutritional applications". Algal Research 81 (2024): 103572. http://dx.doi.org/10.1016/j.algal.2024.103572.
    10.1016/j.algal.2024.103572
  6. Gonçalo Espírito Santo; Ana Barros; Margarida Costa; Hugo Pereira; Mafalda Trovão; Helena Cardoso; Bernardo Carvalho; et al. "Scenedesmus rubescens Heterotrophic Production Strategies for Added Value Biomass". Marine Drugs (2023): https://doi.org/10.3390/md21070411.
    10.3390/md21070411
  7. Tatiana Maurício; Daniela Couto; Diana Lopes; Tiago Conde; Rita Pais; Joana Batista; Tânia Melo; et al. "Differences and Similarities in Lipid Composition, Nutritional Value, and Bioactive Potential of Four Edible Chlorella vulgaris Strains". Foods (2023): https://doi.org/10.3390/foods12081625.
    10.3390/foods12081625
  8. Nádia Correia; Hugo Pereira; Peter S. C. Schulze; Monya M. Costa; Gonçalo E. Santo; Inês Guerra; Mafalda Trovão; et al. "Heterotrophic and Photoautotrophic Media Optimization Using Response Surface Methodology for the Novel Microalga Chlorococcum amblystomatis". Applied Sciences (2023): https://doi.org/10.3390/app13042089.
    10.3390/app13042089
  9. Mafalda Trovão; Lisa M. Schüler; Adriana Machado; Gabriel Bombo; Sofia Navalho; Ana Barros; Hugo Pereira; et al. "Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production". Marine Drugs 20 7 (2022): 440-440. https://doi.org/10.3390/md20070440.
    10.3390/md20070440
  10. Mafalda Trovão; Hugo Pereira; Margarida Costa; Adriana Machado; Ana Barros; Maria Soares; Bernardo Carvalho; et al. "Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production". Applied Sciences 10 7 (2020): 2500-2500. https://doi.org/10.3390/app10072500.
    Published • 10.3390/app10072500
  11. Machado, Adriana; Pereira, Hugo; Costa, Margarida; Santos, Tamára; Carvalho, Bernardo; Soares, Maria; Quelhas, Pedro; et al. "Development of an Organic Culture Medium for Autotrophic Production of Chlorella vulgaris Biomass". Applied Sciences 10 6 (2020): 2156. http://dx.doi.org/10.3390/app10062156.
    Published • 10.3390/app10062156
  12. Lisa Schüler; Mafalda Trovão; Etiele Morais; Adriana Machado; Bernardo Carvalho; Mariana Carneiro; Inês Maia; et al. "Isolation and characterization of novel Chlorella vulgaris mutants with low chlorophyll and improved protein contents for food applications". Front. Bioeng. Biotechnol. (2020): https://www.frontiersin.org/articles/10.3389/fbioe.2020.00469/abstract.
    Accepted • 10.3389/fbioe.2020.00469
  13. Trovão, Mafalda; Pereira, Hugo; Silva, Joana; Páramo, Jaime; Quelhas, Pedro; Santos, Tamára; Silva, Joana T.; et al. "Growth performance, biochemical composition and sedimentation velocity of Tetraselmis sp. CTP4 under different salinities using low-cost lab- and pilot-scale systems". Heliyon 5 5 (2019): e01553. http://dx.doi.org/10.1016/j.heliyon.2019.e01553.
    Published • 10.1016/j.heliyon.2019.e01553
  14. Quelhas, Pedro M.; Trovão, Mafalda; Silva, Joana T.; Machado, Adriana; Santos, Tamára; Pereira, Hugo; Varela, João; Simões, Manuel; Silva, Joana L.. "Industrial production of Phaeodactylum tricornutum for CO2 mitigation: biomass productivity and photosynthetic efficiency using photobioreactors of different volumes". Journal of Applied Phycology 31 4 (2019): 2187-2196. http://dx.doi.org/10.1007/s10811-019-1750-0.
    Published • 10.1007/s10811-019-1750-0
Thesis / Dissertation
  1. Santos, Mafalda Trovão dos. "Heterotrophic growth of thraustochytrids strains: screening, optimization and scale-up". Master, 2019. http://hdl.handle.net/10362/87630.
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022 - 2023 Optimization of a novel microalgal industrial strain’s growth performance and biochemical profile for nutritional applications
Co-supervisor
 Biotecnologia (Master)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022 - 2022 Development of novel Scenedesmus rubescens mutants with improved pigmentation and protein contents
Co-supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2016/03 - 2016/03 IX Encontro Nacional de Estudantes de Bioquímica (ENEBIOQ) (2016/03/18 - 2016/03/21)
Meeting (Co-organisor)
 Universidade de Coimbra, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2016/11/25 - 2016/11/25 Molecular pathways linking inflammation and cancer: from bench to bedside - Prof. Alberto Mantovani
Seminar
 2016 IBP Seminar Series
Istituto di Biochimica delle Proteine Consiglio Nazionale delle Ricerche, Italy

Association member

Society Organization name Role
2014 - 2016 Núcleo de Estudantes de Bioquímica da Universidade de Coimbra Colaboradora do Pelouro de Intervenção cívica e ambiental; Vice-coordenadora do Pelouro de Saídas Profissionais

Journal scientific committee

Journal title (ISSN) Publisher
2025 - 2025 Journal of Applied Phycology (1573-5176) Springer Nature
2025 - 2025 Scientific Reports (2045-2322) Springer Nature
2023 - 2023 Algal Research (2211-9264) Elsevier
Distinctions

Award

2019 Best Poster - Microbiotec'19
Universidade de Coimbra, Portugal