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Sílvia Filipa Vicente Gregório. Completed the Mestrado in Engenharia Biológica in 2009 by Universidade do Algarve, Licenciatura in Ciências de Engenharia Biológica in 2008/12/09 by Universidade do Algarve Faculdade de Ciências e Tecnologia and Doktor (PhD) in Marine Resources in 2017 by Universidad de Cádiz Facultad de Ciencias del Mar y Ambientales. Is Postdoc in Centro de Ciências do Mar. Published 18 articles in journals. Has 1 section(s) of books. Participated in 7 event(s). Has received 4 awards and/or honors. Participates and/or participated as PhD Student Fellow in 1 project(s), Post-doc in 1 project(s) and Research Fellow in 3 project(s). Works in the area(s) of Natural sciences with emphasis on Biological Sciences with emphasis on Marine Biology. In their professional activities interacted with 64 collaborator(s) co-authorship of scientific papers. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Flow Cytometry ; cell proliferation; bioinformatics; R; molecular biology; PCR; aquaculture; microscopy; fluorescence; confocal; image acquisition; image processing; recombinant protein ; purification; sds-page; Western blotting; immunogenetics; Electrophysiology; pH-stat ; ocean acidification; intestine; cell culture; Immunohistochemistry (IHC) ; real time PCR; dna recombinant technology; physiology; bicarbonate secretion; Epithelial  transport; Marine  Biology ; Animal  Physiology; Endocrinology ; " feed formulation"; "permeability"; "salmon"; .
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Identification

Personal identification

Full name
Sílvia Filipa Vicente Gregório

Citation names

  • SF Gregório
  • Sílvia F. Gregório

Author identifiers

Ciência ID
BF14-020B-4C39
ORCID iD
0000-0002-6648-5122

Email addresses

  • sfgregorio@ualg.pt (Professional)

Addresses

  • Centro de Ciências do Mar - Campus de Gambelas, Universidade do Algarve, 8005-139, Faro, Faro, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Marine Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2)
Education
Degree Classification
2018 - 2018
Concluded
 Advanced  Course  in  Animal  Experimentation (Curso de Especialização Tecnológica)
Universidade do Porto Centro Interdisciplinar de Investigação Marinha e Ambiental, Portugal
2017
Concluded
 Marine Resources (Doktor (PhD))
Universidad de Cádiz Facultad de Ciencias del Mar y Ambientales, Spain
"REGULATION OF BICARBONATE TRANSPORTER CASCADE IN THE INTESTINE OF MARINE FISH" (THESIS/DISSERTATION)
 Sobresaliente cum laude
2009
Concluded
 Engenharia Biológica (Mestrado)
Universidade do Algarve, Portugal
"Molecular and cellular analysis of the regenerative response of skin in a fish model" (THESIS/DISSERTATION)
 13
2002 - 2008/12/09
Concluded
 Ciências de Engenharia Biológica (Licenciatura)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
 12
Affiliation

Science

Category
Host institution 		Employer
2017/02 - Current Postdoc (Research) Centro de Ciências do Mar, Portugal
Centro de Ciências do Mar, Portugal
2015 - 2017 Research Trainee (Research) Fundação para a Ciência e a Tecnologia, Portugal
Centro de Ciências do Mar, Portugal
(...)
2013 - 2015 Research Assistant (Research) Centro de Ciências do Mar, Portugal
Centro de Ciências do Mar, Portugal
2010 - 2012 Research Assistant (Research) Centro de Ciências do Mar, Portugal
Centro de Ciências do Mar, Portugal
2008/01 - 2009/03 Research Trainee (Research) Universidade do Algarve, Portugal
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal

Others

Category
Host institution 		Employer
2009 - 2009/06 Voluntariado Universidade do Algarve, Portugal
Projects

Grant

Designation Funders
2022/10/01 - 2023/12/31 Improve Atlantic salmon robustness in freshwater phase through training and feeding to a successfully timed sea transfer
320566
Post-doc
Universitetet i Bergen, Norway
 Norwegian Research Council
Ongoing
2016/01 - 2019/07 Physiological Response of Fish to Ocean Acidification
PTDC/MAR-BIO/3034/2014
Research Fellow
Centro de Ciências do Mar, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2015 - 2017 Regulation of bicarbonate transporter cascade in the intestine of marine fish
SFRH/BD/113363/2015
PhD Student Fellow
Centro de Ciências do Mar, Portugal

Universidad de Cádiz Facultad de Ciencias del Mar y Ambientales, Spain
Fundação para a Ciência e a Tecnologia
Concluded
2015 - 2017 Regulation of bicarbonate transporter cascade in the intestine of marine fish
SFRH/BD/113363/2015
 Fundação para a Ciência e a Tecnologia
2013 - 2015 FISH HORMONES AND THE OCEAN CARBON
PTDC/MAR-BIO/3811/2012
Research Fellow
Centro de Ciências do Mar, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010 - 2012 Biomineralisation in seawater fish intestine: endocrine perspective
PTDC/MAR/104008/2008
Research Fellow
Centro de Ciências do Mar, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2024/01/01 - 2024/12/31 Fundo Ambiental
na
Researcher
Outputs

Publications

Book chapter
  1. SF Gregório; Martos-Sitcha JA; Fuentes, J.. "Vasotocinergic system in the gilthead sea bream (Sparus aurata): from molecular to electrophysiological characterization in the gastrointestinal tract". In Avances en Endocrinología Comparada, edited by M.J. Delgado; A.L. Alonso-Gómez,; N. De Pedro; E. Irsona, 167-171. Spain: Complutense University of Madrid, 2012.
    Published
Conference poster
  1. Paula Simó-Mirabet; SF Gregório. Corresponding author: Paula Simó-Mirabet. "IMPROVING GROWTH BY BIOTECHNOLOGICAL TREATMENTS OF VEGETABLE INGREDIENTS IN AQUAFEEDS FOR Seriola dumerili: INSIGHTS ON INTESTINAL FUNCTION, IMMUNE RESPONSE AND MICROBIOTA". Paper presented in Aquaculture Europe 2023, 2023.
  2. SF Gregório. "Regulation of bicarbonate secretion in marine fish intestine via the calcium sensing receptor". Paper presented in Society of Experimental Biology - Annual Meeting, 2017.
  3. SF Gregório; Fuentes, J.; I Ruiz-Jarabo. "The Impact of Ocean Acidification in Sea bream Intestinal Physiology". Paper presented in Annual Meeting of Society Experimental Biology, 2016.
  4. SF Gregório; Carvalho, E. S. M.; Power DM; Canario, A. V. M.; Fuentes, J.. "Regulation of bicarbonate transporters by calcitropic hormones in the intestine of marine fish". Paper presented in 8º CONGRESO ASOCIACIÓN IBÉRICO DE ENDOCRINOLOGÍA COMPARADA, 2011.
  5. SF Gregório; Carvalho, E. S. M.; Power, D. M.; Canario, A. V. M.; Fuentes, J.. "cAMP and PTHrP regulate water absorption and bicarbonate secretion in the sea bream intestine". Paper presented in 8º CONGRESO ASOCIACIÓN IBÉRICO DE ENDOCRINOLOGÍA COMPARADA, 2011.
  6. SF Gregório; Vieira FA; Canario, A. V. M.; Power, D. M.. "Analysis of sea bream skin and scale regeneration process using a microarray approach". Paper presented in 8º CONGRESO ASOCIACIÓN IBÉRICO DE ENDOCRINOLOGÍA COMPARADA., 2011.
Journal article
  1. Fuentes, J.; SF Gregório; Fonseca, F.; Robles-Arozarena, R.; Martos-Sitcha, J. A.; Moyano, F. J.. Corresponding author: Moyano, F. J.. "Effect of bile salts on intestinal epithelial function in gilthead seabream (Sparus aurata)". Fish Physiology and Biochemistry 50 4 (2024): 1777-1790. http://dx.doi.org/10.1007/s10695-024-01369-8.
    10.1007/s10695-024-01369-8
  2. Leal, Esther; Angotzi, Anna Rita; Godino-Gimeno, Alejandra; SF Gregório; Rotllant, Josep; Saera-Vila, Alfonso; Fuentes, Juan; Cerdá-Reverter, José Miguel. "Agouti overexpression in a transgenic model regulates integrity, permeability and electrogenic amino acid transport in zebrafish intestine". Frontiers in Marine Science 9 (2022): http://dx.doi.org/10.3389/fmars.2022.1011702.
    10.3389/fmars.2022.1011702
  3. Leal, E.; Angotzi, A.R.; Gregório, S.F.; Ortiz-Delgado, J.B.; Rotllant, J.; Fuentes, J.; Tafalla, C.; Cerdá-Reverter, J.M.. "Role of the melanocortin system in zebrafish skin physiology". Fish & Shellfish Immunology 130 (2022): 591-601. http://dx.doi.org/10.1016/j.fsi.2022.09.036.
    10.1016/j.fsi.2022.09.036
  4. Ruiz-Jarabo, Ignacio; Gregório, Silvia F.; Fuentes, Juan. "Regulation of Stanniocalcin Secretion by Calcium and PTHrP in Gilthead Seabream (Sparus aurata)". Biology 11 6 (2022): 863. http://dx.doi.org/10.3390/biology11060863.
    10.3390/biology11060863
  5. Barany, Andre; Oliva, Milagrosa; SF Gregório; Martínez-Rodríguez, Gonzalo; Mancera, Juan Miguel; Fuentes, Juan. "Dysregulation of Intestinal Physiology by Aflatoxicosis in the Gilthead Seabream (Sparus aurata)". Frontiers in Physiology 12 (2021): http://dx.doi.org/10.3389/fphys.2021.741192.
    10.3389/fphys.2021.741192
  6. Ruiz-Jarabo, I.; SF Gregório; Alves, A.; Mancera, J.M.; Fuentes, J. "Ocean acidification compromises energy management in Sparus aurata (Pisces: Teleostei)". Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology (2021): 110911. http://dx.doi.org/10.1016/j.cbpa.2021.110911.
    10.1016/j.cbpa.2021.110911
  7. Alexandra Alves; SF Gregório; Ignacio Ruiz-Jarabo; Fuentes, J. "Intestinal response to ocean acidification in the European sea bass (Dicentrarchus labrax).". Comparative biochemistry and physiology. Part A, Molecular & integrative physiology (2020): https://doi.org/10.1016/j.cbpa.2020.110789.
    10.1016/j.cbpa.2020.110789
  8. Alexandra Alves; SF Gregório; Renata C. Egge; Fuentes, J. "Molecular and functional regionalization of bicarbonate secretion cascade in the intestine of the European sea bass (Dicentrarchus labrax)". Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology (2019): http://dx.doi.org/10.1016/j.cbpa.2019.03.017.
    10.1016/j.cbpa.2019.03.017
  9. SF Gregório; Ignacio Ruiz-Jarabo; Edison S.M. Carvalho; Fuentes, J. "Increased intestinal carbonate precipitate abundance in the sea bream (Sparus aurata L.) in response to ocean acidification". PLOS ONE (2019): http://dx.doi.org/10.1371/journal.pone.0218473.
    10.1371/journal.pone.0218473
  10. SF Gregório; Fuentes, J. "Regulation of Bicarbonate Secretion in Marine Fish Intestine by the Calcium-Sensing Receptor". International Journal of Molecular Sciences (2018): http://www.mdpi.com/1422-0067/19/4/1072.
    10.3390/ijms19041072
  11. I. Ruiz-Jarabo; SF Gregório; P. Gaetano; F. Trischitta; J. Fuentes. "High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)". Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology (2017):
    doi:10.1016/j.cbpa.2017.02.022.
  12. SF Gregório; Edison S.M. Carvalho; Adelino V.M. Canário; Deborah M. Power; Juan Fuentes. "PTHrP regulates water absorption and aquaporin expression in the intestine of the marine sea bream (Sparus aurata, L.)". General and Comparative Endocrinology 213 (2015): 24-31. http://dx.doi.org/10.1016/j.ygcen.2014.12.011.
    10.1016/j.ygcen.2014.12.011
  13. SF Gregório; Edison S.M. Carvalho; Marco A. Campinho; Deborah M. Power; Canario, A. V. M.; Fuentes, J. "Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP". The Journal of Experimental Biology (2014):
    doi:10.1242/jeb.098517
  14. Martos-Sitcha JA; Gregório SF; Carvalho ES; Canario AV; Power DM; Mancera JM; Martínez-Rodríguez G; Fuentes J. "AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata).". (2013): http://europepmc.org/abstract/med/23973797.
    10.1016/j.ygcen.2013.07.017
  15. SF Gregório; Carvalho ES; Encarnação S; Wilson JM; Power DM; Canário AV; Fuentes J. "Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.).". (2013): http://europepmc.org/abstract/med/23038737.
    10.1242/jeb.073742
  16. Carvalho ES; Sílvia F. Gregório; Power DM; Canário AV; Fuentes J. "Water absorption and bicarbonate secretion in the intestine of the sea bream are regulated by transmembrane and soluble adenylyl cyclase stimulation.". (2012): http://europepmc.org/abstract/med/22752677.
    10.1007/s00360-012-0685-4
  17. Ferlazzo, A.; Carvalho, E. S. M.; SF Gregório; Power, D. M.; Canario, A. V. M.; Trischitta, F.; Fuentes, J.. "Prolactin regulates luminal bicarbonate secretion in the intestine of the sea bream (Sparus aurata L.)". Journal of Experimental Biology 215 21 (2012): 3836-3844. http://dx.doi.org/10.1242/jeb.074906.
    10.1242/jeb.074906
  18. Estêvão MD; Silva N; Redruello B; Costa R; SF Gregório; Canário AV; Power DM. "Cellular morphology and markers of cartilage and bone in the marine teleost Sparus auratus.". (2011): http://europepmc.org/abstract/med/21234603.
    10.1007/s00441-010-1109-y
  19. Vieira FA; SF Gregório; Ferraresso S; Thorne MA; Costa R; Milan M; Bargelloni L; et al. "Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus.". (2011): http://europepmc.org/abstract/med/21981800.
    10.1186/1471-2164-12-490
Thesis / Dissertation
  1. SF Gregório. "Regulation Of Bicarbonate Transporter Cascade In The Intestine Of Marine Fish". PhD, Universidad de Cádiz Facultad de Ciencias del Mar y Ambientales, 2017.
  2. SF Gregório. "Molecular and cellular analysis of the regenerative response of skin in a fish model". Master, Universidade do Algarve Faculdade de Ciências e Tecnologia, 2009.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/11/22 Implicación del sistema de melanocortinas en la fisiología de la piel del pez cebra (Danio rerio) XVIII Congreso Nacional de Acuicultura “Acuicultura: mares y ríos de oportunidades”
2022/11/21 Melanocortinas y su función intestinal en peces XVIII Congreso Nacional de Acuicultura
Sociedad Española de Acuicultura (SEA) (Cdiz, Spain)
2018/06/12 The importance of regulating calcium transport is that fish are able to regulate HCO3- secretion and are able to accommodate this process to their physiological status, potentially increasing their capacity of production of buffering alkaline carbonates. The natural follow up of this study focussed on the putative role of the intestinal calcium sensing receptor on the regulation of apical HCO3- secretion in the marine fish intestine, VII CONGRESS OF THE IBERIAN SOCIETY FOR ICTHYOLOGY
IBERIAN SOCIETY FOR ICTHYOLOGY (Faro, Portugal)
2017/07/03 Regulation of bicarbonate secretion in the marine fish intestine via the calcium sensing receptor Annual Metting 2017 - Sweden
(Sweden)
2016 Vivo-morpholinos against claudin-15 show osmoregulatory functions of tight-junction regulation in killifish 12th International Congress on the Biology of Fish
American Fisheries Society (San Marco, United States)
2015 An approach to Stanniocalcin secretion in gilthead seabream (Sparus aurata) X Congreso Asociación Ibérica de Endocrinología Comparada
Asociación Ibérica de Endocrinología Comparada (AIEC) (Castellón, Spain)
2011/09 Vasotocinergic system in the gilthead sea bream (Sparus aurata): From molecular to electrophysiological characterization in the gastrointestinal tract 8º CONGRESO ASOCIACIÓN IBÉRICO DE ENDOCRINOLOGÍA COMPARADA.
ASOCIACIÓN IBÉRICO DE ENDOCRINOLOGÍA COMPARADA (Madrid, Spain)
2011/04 Biomineralization in marine fish intestine is hormone regulated. II Interdisciplinary Approaches in Fish Skeletal Biology
Fish Skeletal Biology (Tavira, Portugal)
2011 Endocrine control of intestinal mineralization in marine fish 8º CONGRESO ASOCIACIÓN IBÉRICO DE ENDOCRINOLOGÍA COMPARADA
ASOCIACIÓN IBÉRICO DE ENDOCRINOLOGÍA COMPARADA (Madrid, Spain)

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2019/11/25 - 2019/11/29 Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.
Other
 Flow Cytometry Course
Universidade do Algarve Centro de Investigação em Biomedicina, Portugal
2017/10 - 2017/10 Assess the role of bioinformatics in molecular life-science research. Describe the key features of primary and secondary databases.
Workshop
 EMBL-EBI Bioinformatics
Centro de Ciências do Mar, Portugal
2017/06 - 2017/06 R for absolute Beginners, concepts and practical example.
Workshop
 R for absolute Beginners 5º Edition
Centro de Ciências do Mar, Portugal
2016/02/01 - 2016/02/05 Manuscript Submission and Review.
Workshop
 Mares Conference on Marine Ecosystems Health and Conservation
2014/11 - 2014/11 Course in Molecular Biology Techniques Applied to Aquaculture
Workshop
 Course in Molecular Biology Techniques Applied to Aquaculture
Universidad de Cádiz Departamento de Biología, Spain
2014/01 - 2014/01 Image acquisition and pitfalls; Bright field microscopy; Fluorescence and confocal microscopy and Sample preparation and image processing.
Workshop
 Course: II Introduction Workshop to light microscopy
Universidade do Algarve Centro de Investigação em Biomedicina, Portugal
2012/07 - 2012/07 The aim of the course is to provide an in-depth evaluation of strategies to achieve maximum optimization of recombinant protein production and purification experiments.
Workshop
 Course: On strategies for recombinant protein production
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal

Consulting

Activity description Institution / Organization
2020/06/02 - Current The  gut  is  directly  exposed  to  external  factors  like  feed,  water  and  microbiota,  and   functions  as  a  selective  barrier  allowing  nutrients  in  and  denying  entry  to  pathogens.  As  other   epithelia,  the  gut  displays  two  distinct  features:  polarity  and  tightness.   Epithelial electrophysiology in Ussing chambers: Tissue Resistance as an indicator of intestinal integrity. Permeability measurement using fluorescent methods to characterize dietary modifications of intestine. Testing  Blue  S.L. , Spain
2021/01/18 - 2021/01/22 Epithelial electrophysiology in Ussing chambers: Tissue Resistance as an indicator of intestinal integrity. Permeability measurement using fluorescent methods to characterize dietary modifications of intestinal paracellular function in Argyrosomus regius. IPMA - Estação Piloto de Piscicultura em Olhão (EPPO), Portugal
2019/11/02 - 2019/12/15 Epithelial electrophysiology in Ussing chambers: Tissue Resistance as an indicator of intestinal integrity. Permeability measurement using fluorescent methods to characterize the gut transporte and intestinal paracellular function in Sepia officinalis. Universidade do Algarve, Portugal
2009 - 2009 Volunteer researcher. Comparative and Molecular Endocrinology Group, University of Algarve. Handling and techniques with animals. Practical experience: Industrial Monitoring – Anatomy and morphology: tissue/animal processing; Malformation identification in several species (i.e. Rainbow trout; Sea bream; Sea bass; Cod).

Mentoring / Tutoring

Topic Student name
2019/12 - Current External evaluation commission PhD student. Pasqualina Gaetano
2023/03/01 - 2023/04/16 During internship, I acted as a supervisor to the student. CArolina
2023/03/01 - 2023/04/10 During one-month internship, I acted as a supervisor to the student. Rute
2022/09/01 - 2022/12/31 supervisor student PhD Anyell

Other jury / evaluation

Activity description Institution / Organization
2015/06 - 2015/07 Program Ciência Viva: Within this program, high school students are hosted for 2 weeks in the laboratory to have your first experience in research.

Scientific expedition

Activity description Institution / Organization
2022/06/01 - 2022/07 Epithelial electrophysiology in Ussing chambers: Tissue Resistance as an indicator of intestinal integrity. Permeability measurement using fluorescent methods to characterize dietary modifications of intestinal paracellular function in Salmon salar. Universitetet i Bergen, Norway
Distinctions

Award

2017 Travel Grant
Society for Experimental Biology, United Kingdom
2016 Travel Grant
Society for Experimental Biology, United Kingdom
2015 PhD Research Scholarships
Fundação para a Ciência e a Tecnologia, Portugal
2008 Mobility Grant