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Mariana Gonçalves graduated in Biology in 2018 at the Trás-os-Montes and Alto Douro University (UTAD). In 2022, she completed her masters in Clinical Laboratory Biology from UTAD. In 2020, for 3 months, she was a research fellow funded by FCT in the Practical Course - Application of biological models to ecotoxicology studies. She was a research fellow of the project Exploring in vivo U1 snRNA splicing modulation as an alternative therapy for Mucopolysaccharidosis IIIC funded by the National MPS Society USA and which is being developed at the National Health Institute Dr. Ricardo Jorge Porto (INSA). Currently, she is on a PhD fellow funded by FCT entitled "Innovative & personalized RNA-based therapies for rare diseases and development of models for their testing: application to Mucolipidosis II in the Lysosomal Storage Disorders group at INSA. She is also part of two FCT Research Units, the first the Center for Animal Science Studies (CECA; ICETA, University of Porto) which now integrates the new associated laboratory AL4animals and the second Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB; University of Trás-os-Montes and Alto Douro). Mariana Gonçalves is co-author of 5 articles published in indexed journals, and 1 article in the Portuguese journal of Zootechnics. She also presented 30 oral communications, 9 as first author, authored or co-authored 51 poster communications. In 2021 she received 1 best oral communication award at the 17 th International Symposium of the Portuguese Society of Metabolic Disorders and in 2023 she received 1 best conference Poster, Dia do Jovem Investigador.
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Identification

Personal identification

Full name
Mariana Mendes Gonçalves
Date of birth
1996/02/26
Gender
Female

Citation names

  • Gonçalves, Mariana

Author identifiers

Ciência ID
BD11-B5FB-4AD7
ORCID iD
0000-0002-3111-4612

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language Speaking Reading Writing Listening Peer-review
English Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2023/10/01 - 2027
Ongoing
 Ciências Químicas e Biológicas (Doutoramento)
Major in Biologia
Universidade de Trás-os-Montes e Alto Douro, Portugal
"Innovative & personalized RNA-based therapies for rare diseases and development of models for their testing: application to Mucolipidosis II" (THESIS/DISSERTATION)
2023/01 - 2023/04
Concluded
 Curso “Ética e Ciência” (Curso de Especialização Tecnológica)
Major in Biologia
Universidade de Trás-os-Montes e Alto Douro, Portugal
 19 valores
2022/10 - 2022/10
Concluded
 Training School - Introduction to Nucleic Acid Therapy, COST Action CA17103 - DARTER, Delivery of RNA Antisense Therapeutics (Curso de Especialização Tecnológica)
Major in Biologia
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2022/03/25
Concluded
 Biologia Clínica Laboratorial (Mestrado)
Universidade de Trás-os-Montes e Alto Douro Escola de Ciências da Vida e do Ambiente, Portugal
"In vivo validation studies of RNA therapeutic approaches for Lysosomal storage diseases" (THESIS/DISSERTATION)
 17
2019/07/08 - 2019/07/12
Concluded
 Animal Science course Category B, accredited by FELASA (mouse, rat, zebrafish and fish species specific) (Outros)
Universidade de Trás-os-Montes e Alto Douro, Portugal
 Aprovado
2018/07/18
Concluded
 Biologia (Licenciatura)
Universidade de Trás-os-Montes e Alto Douro, Portugal
"Evaluation of the effect of feeding frequency in juvenile Tenca (TINCA TINCA)" (THESIS/DISSERTATION)
 12
Affiliation

Others

Category
Host institution 		Employer
2023/01/01 - 2027/01/01 PhD fellow Fundação para a Ciência e a Tecnologia, Portugal
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
(...)
2021/01/20 - 2022/12/31 Research fellow National MPS Society, United States
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2020/08/21 - 2020/10/30 Research fellow Fundação para a Ciência e a Tecnologia, Portugal
Universidade de Trás-os-Montes e Alto Douro, Portugal
Projects

Grant

Designation Funders
2023/01/01 - Current Innovative & personalized RNA-based therapies for rare diseases and development of models for their testing: application to Mucolipidosis II
2022.13676.BD
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/20 - 2022/12/31 Exploring in vivo U1 snRNA splicing modulation as an alternative therapy for MPS IIIC
2019DGH1642
Research Fellow
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
 National MPS Society
Concluded
2020/08/21 - 2020/10/30 Practical Course-Application of biological models to ecotoxicology studies
BI/UTAD/23/2020
Research Fellow
Universidade de Trás-os-Montes e Alto Douro, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2023/05/02 - 2024/04/01 Addressing a challenging enzyme in vitro: proof of principle on the therapeutic potential of an antisense oligonucleotide approach for Mucolipidosis II
AL4A-PROJ-LT3.5
Researcher
Al4animalS - Associate Laboratory for Animal Science and Veterinary, Portugal
 Al4animalS - Associate Laboratory for Animal Science and Veterinary
Ongoing
Outputs

Publications

Conference poster
  1. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. An Antisense Oligonucleotide based therapy for a rare disease: in vitro and in vivo studies. Final Meeting of the COST Action “Delivery of Antisense RNA therapies” (DARTER) – CA17103, Bilbao - Spain, February 2023. §These authors contributed equally to the work. *Presenting author.". 2023.
  2. Gonçalves, Mariana. "Moreira L*, Coutinho MF, Carvalho S, Duarte AJ, Ribeiro D, Santos JI, Gonçalves M, Gaspar P, Amaral O, Encarnação M, Matos L, Neuparth T, Soares J, Ribeiro M, Santos M, Alves S. Pluripotent cells and zebrafish: two complementary platforms for modelling Lysosomal Storage Disorders. Final meeting of the COST Action “Delivery of Antisense RNA Therapeutics” (DARTER) CA17103, Bilbao-*Presenting author". 2023.
  3. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Developing models and an RNA-based therapy for a rare disease. 2nd Meeting of the Associate Laboratory for Animal and Veterinary Sciences (AL4animalS), Vila Real - Portugal, May 2023. §These authors contributed equally to the work. *Presenting author.". 2023.
  4. Moreira, L; Maria Francisca Coutinho; Carvalho, Sofia; Duarte, Ana Joana; Ribeiro, Diogo; Santos, Juliana Inês; Gonçalves, Mariana; et al. Corresponding author: Moreira, L. "Pluripotent cells and zebrafish: two complementary platforms for modelling Lysosomal Storage Disorders". Paper presented in CIISA Congress 2022, 2022.
  5. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. A Personalized RNA-based therapy for Mucolipidosis II: in vitro and in vivo studies. III International Meeting of the Portuguese Society of Genetics (SPG), Évora – Portugal, June 2022. §These authors contributed equally to the work. *Presenting author.". 2022.
  6. Gonçalves, Mariana. "Matos L§*, Gonçalves M§, Santos JI§, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Exploring U1 snRNA splicing modulation as an alternative therapy for MPS IIIC: in vitro and in vivo studies. III International Meeting of the Portuguese Society of Genetics (SPG), Évora – Portugal, June 2022. §These authors contributed equally to the work. *Presenting author.". 2022.
  7. Gonçalves, Mariana. "Moreira L*, Santos JI, Gonçalves M, Carvalho S, Encarnação M, Coutinho MF, Matos L, Alves S. Urine Stem Cells as a potential in vitro model for Mucolipidosis type II. III International Meeting of the Portuguese Society of Genetics (SPG), Évora – Portugal, June 2022. *Presenting author.". 2022.
  8. Gonçalves, Mariana. "Matos L§*, Gonçalves M§, Santos JI, Coutinho MF, Prata MJ, Omidi M, Pohl S, Alves S. A Personalized antisense oligonucleotide exon-skipping therapeutic approach for Mucolipidosis II. 24th ESGLD (European Study Group on Lysosomal Diseases) Workshop, Lancaster – United Kingdom, September 2022. §These authors contributed equally to the work. *Presenting author.". 2022.
  9. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI§, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Innovative methodologies to generate mice expressing in the liver human splicing mutations: good model to test in vivo the efficacy of modified U1 snRNAs. 24th ESGLD (European Study Group on Lysosomal Diseases) Workshop, Lancaster – United Kingdom, September 2022. §These authors contributed equally to the wo". 2022.
  10. Gonçalves, Mariana. "Santos JI*, Gonçalves M, Matos L, Gaspar P, Pires MJ, Oliveira P, Prata MJ, Coutinho MF, Alves S. RNA as a therapeutic tool in Mucopolysaccharidoses. 24th ESGLD (European Study Group on Lysosomal Diseases) Workshop, Lancaster – United Kingdom, September 2022. *Presenting author.". 2022.
  11. Gonçalves, Mariana. "Moreira L*, Santos JI, Gonçalves M, Carvalho S, Encarnação M, Coutinho MF, Matos L, Alves S. Urine Stem Cells as a potential in vitro model for Mucolipidosis type II. 24th ESGLD (European Study Group on Lysosomal Diseases) Workshop, Lancaster – United Kingdom, September 2022. *Presenting author.". 2022.
  12. Gonçalves, Mariana. "Carvalho S*, Santos JI, Moreira L, Gaspar P, Gonçalves M, Matos L, Encarnação M, Ribeiro D, Duarte AJ, Prata MJ, Coutinho MF, Alves S. Baby teeth patient-derived stem cells: an innovative model to SHED light over MPS pathology. 24th ESGLD (European Study Group on Lysosomal Diseases) Workshop, Lancaster – United Kingdom, September 2022. *Presenting author.". 2022.
  13. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. An Antisense Oligonucletide based therapy for a rare disease: in vitro and in vivo studies. 3rd day of young researcher of National Health Institute Dr. Ricardo Jorge, Lisbon - Portugal, November 2022. §These authors contributed equally to the work. *Presenting author.". 2022.
  14. Gonçalves, Mariana. "Carvalho S*, Santos JI, Moreira L, Gaspar P, Gonçalves M, Matos L, Encarnação M, Ribeiro D, Duarte AJ, Prata MJ, Coutinho MF, Alves S. The disease modelling value of baby teeth: A new way to unlock knowledge about a special group of genetic disorders. 3rd day of young researcher of National Health Institute Dr. Ricardo Jorge, Lisbon - Portugal, November 2022. *Presenting author.". 2022.
  15. Gonçalves, Mariana. "Moreira L*, Coutinho MF, Carvalho S, Duarte AJ, Ribeiro D, Santos JI, Gonçalves M, Gaspar P, Amaral O, Encarnação M, Matos L, Neuparth T, Soares J, Ribeiro M, Santos M, Alves S. Pluripotent cells and zebrafish: two complementary platforms for modelling Lysosomal Storage Disorders. Congress of Center for Interdisciplinary Research in Animal Health (CIISA) – Innovation in Animal, Veterinary and Biom". 2022.
  16. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI§, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Generation of mice expressing in the liver human splicing mutations: a good model to test in vivo the therapeutic efficacy of modified U1 snRNAs for Mucopolysaccharidosis type IIIC. 26th Annual Meeting of the Portuguese Society of Human Genetics (SPGH), Coimbra – Portugal, November 2022. §These authors contr". 2022.
  17. Gonçalves, Mariana. "Carvalho S*, Santos JI, Moreira L, Gaspar P, Gonçalves M, Matos L, Encarnação M, Ribeiro D, Duarte AJ, Prata MJ, Coutinho MF, Alves S. When folklore meets science: the 21st century baby teeth collector that is helping us SHED light over rare genetic disorders. 26th Annual Meeting of the Portuguese Society of Human Genetics (SPGH), Coimbra – Portugal, November 2022. *Presenting author.". 2022.
  18. Gonçalves, Mariana. "Santos JI *, Gonçalves M, Matos L, Gaspar P, Pires MJ, Oliveira P, Prata MJ, Coutinho MF, Alves S. Assessing the potential of RNA-based therapeutics for a group of Lysosomal Storage Diseases with neurological involvement. 25th Annual Meeting of the Portuguese Society of Human Genetics (SPGH), *Presenting author,". 2021.
  19. Gonçalves, Mariana. "Matos L§*, Gonçalves M§ Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Antisense oligonucleotide exon-skipping as a therapeutic approach for Mucolipidosis type II a/ß: in vitro and in vivo studies. 25th Annual Meeting of the Portuguese Society of Human Genetics (SPGH). Online meeting. §These authors contributed equally to the work. *Presenting author". 2021.
  20. Gonçalves, Mariana. "Matos L§*, Gonçalves M§ Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Antisense oligonucleotide exon-skipping as a therapeutic approach for Mucolipidosis type II a/ß: in vitro and in vivo studies. 17th Annual Meeting of the Oligonucleotide Therapeutics Society. Online meeting. §These authors contributed equally to the work. *Presenting author". 2021.
  21. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Antisense oligonucleotide exon-skipping as a therapeutic approach for Mucolipidosis type II a/ß: in vitro and in vivo studies. 1st Symposium on Oligonucleotide Technologies & Therapeutics Portugal OTP 2021. Online meeting. §These authors contributed equally to the work. *Presenting author". 2021.
  22. Gonçalves, Mariana. "Santos JI§*, Matos L§, Gonçalves M§, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. Mutation-adapted U1 snRNA as a therapeutic strategy for Mucopolysaccharidosis IIIC: in vitro and in vivo studies. 1st Symposium on Oligonucleotide Technologies & Therapeutics Portugal OTP 2021. Online meeting. §These authors contributed equally to the work. *Presenting author". 2021.
  23. Gonçalves, Mariana. "Santos JI*, Gonçalves M, Matos L, Gaspar P, Pires MJ, Oliveira PA, Prata MJ, Coutinho MF, Alves S. Targeted RNA-based therapies for Mucopolysaccharidosis. 16th International Symposium on MPS and Related Diseases. Online meeting. *Presenting author". 2021.
  24. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI§, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. Mutation-adapted U1 snRNA as a therapeutic strategy for Mucopolysaccharidosis IIIC: in vitro and in vivo studies. Jornadas da Ciência. University of Trás-os-Montes and Alto Douro. Online meeting. §These authors contributed equally to the work. *Presenting author,". 2021.
  25. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI§, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. Mutation-adapted U1 snRNA as a therapeutic strategy for Mucopolysaccharidosis IIIC: in vitro and in vivo studies. XXIII National meeting of Biology students. Online meeting. §These authors contributed equally to the work. *Presenting author". 2021.
  26. Gonçalves, Mariana. "Silva-Reis R*; Ribeiro C; Gonçalves M; Ferreira T; Martins T; Pires MJ.; Coelho AC.; et al. "A rat model for targeting the gut microbiota dysbiosis in colorectal cancer". Paper presented in 3rd International Conference of the European College of Veterinary Microbiology, *Presenting author ,". 2021.
  27. Gonçalves, Mariana. "Silva-Reis R*; Ribeiro C; Gonçalves M; Ferreira T; Faustino-Rocha AI; Pires MJ; Gama A; Ferreira R; Oliveira P. "Animal model of colorectal cancer: spectrum of dimethylhydrazine induced lesions in the cecum, small intestine and colon of male wistar rats". 2º Expobioterios Virtual, *Presenting author". 2021.
  28. Gonçalves, Mariana. "Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires MJ; et al. "Diet-induced obesity: effects of Ganoderma lucidum supplementation". Paper presented in International Conference on Medicinal and Food Plant Research & 3rd Sino-CPLP Symposium on Natural Products and Biodiversity Resources, , *Presenting author". 2021.
  29. Gonçalves, Mariana. "Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires MJ; et al. "The effects of triterpenic acid and phenolic acid extracted from ganoderma lucidum in an animal model of obesity". Digital Colloquium of Chemistry Students, *Presenting author". 2021.
  30. Gonçalves, Mariana. "Silva-Reis R*; Mendes B.; Castro-Ribeiro C; Gonçalves M; Ferreira T; Gama A; Ferreira R; Oliveira P.. "The Role of 1,2- Dimethylhydrazine and Azoxymethane as Chemical Carcinogens". Digital Colloquium of Chemistry Students. *Presenting author 2021.". 2021.
  31. Gonçalves, Mariana. "Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires MJ; et al. "Ganodema lucidum num modelo animal de obesidade - potencial efeito protetor". XXIII National meeting of Biology students *Presenting author". 2021.
  32. Gonçalves, Mariana. "Silva-Reis R*; Nascimento-Gonçalves E; Castro-Ribeiro C; Gonçalves M; Pires MJ; Ferreira T; Gama A; Ferreira R; Oliveira P.. "Dimethylhydrazine induces aberrant crypt foci formation and epithelial cell proliferation in the colon of Wistar rats". XXIII National meeting of Biology students. *Presenting author". 2021.
  33. Gonçalves, Mariana. "Silva-Reis R*; Castro-Ribeiro C; Gonçalves M; Ferreira T; Iglesias-Aguirre C; Cortés-Martín A; Espín CJ; et al. "Changes in the intestinal microbiome associated with early stages of colorectal cancer in a rat model". Jornadas da Ciência,University of Trás-os-Montes and Alto Douro Vila Real - Portugal *Presenting author". 2021.
  34. Gonçalves, Mariana. "Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires MJ; et al "Preliminary results: the effect of Ganoderma lucidum in an animal model of obesity". Jornadas da Ciência, University of Trás-os-Montes and Alto Douro, Vila Real - Portugal *Presenting author". 2021.
  35. Gonçalves, Mariana. "Gonçalves M*; Silva-Reis R; Medeiros-Fonseca B; Peixoto F; Gaivão I; Matos M; Pinto M; et al. "Eisenia Fetida as a biological research model for soy isoflavones". Natural products application: Health, Cosmetic and Food, *Presenting author". 2021.
  36. Gonçalves, Mariana. "Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires M J; et al. Lee Index Variation and Glucose Clearance Rate in an Animal Model Of Obesity with Animals Supplemented with Ganoderma lucidum. Natural products application: Health, Cosmetic and Food (Online Edition), *Presenting author". 2021.
  37. Gonçalves, Mariana. "Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires MJ; et al. "Glucose intolerance test in C57BL/6 mice an animal model of obesity". 2º Expobioterios virtual, *Presenting author". 2021.
  38. Gonçalves, Mariana; Matos, Liliana; Santos, Juliana Inês; Maria Francisca Coutinho; Prata, Maria João; Pires, Maria João; Oliveira, Paula; Alves, Sandra. "Mutation-adapted U1 snRNA as a therapeutic strategy for Mucopolysaccharidosis IIIC: in vitro and in vivo studies". Paper presented in XXIII Encontro Nacional de Estudantes de Biologia, 2021.
  39. Santos, Juliana Inês; Gonçalves, Mariana; Matos, Liliana; Gaspar, Paulo; Pires, Maria João; Oliveira, Paula; Prata, Maria João; Coutinho, Maria Francisca; Alves, Sandra. "Targeted RNA-based therapies for Mucopolysaccharidosis". Paper presented in 16th International Symposium on MPS and Related Diseases, 2021.
  40. Matos, Liliana; Gonçalves, Mariana; Santos, Juliana Inês; Coutinho, Maria Francisca; Prata, Maria João; Pires, Maria João; Oliveira, Paula; Alves, Sandra. "Antisense Oligonucleotide Exon-skipping As A Therapeutic Approach For Mucolipidosis Type II Alpha/beta: In Vitro And In Vivo Studies". Paper presented in 17th Annual Meeting of the Oligonucleotide Therapeutics Society, 2021.
  41. Santos, Juliana Inês; Gonçalves, Mariana; Matos, Liliana; Gaspar, Paulo; Pires, Maria João; Oliveira, Paula; Prata, Maria João; Coutinho, Maria Francisca; Alves, Sandra. "Assessing the potential of RNA-based therapeutics for a group of Lysosomal Storage Diseases with neurological involvement". Paper presented in 25th Annual Meeting SPGH, 2021.
  42. Matos, Liliana; Gonçalves, Mariana; Santos, Juliana Inês; Coutinho, Maria Francisca; Prata, Maria João; Pires, Maria João; Oliveira, Paula; Alves, Sandra. "Antisense oligonucleotide exon-skipping as a therapeutic approach for Mucolipidosis type II a/b: in vitro and in vivo studies". Paper presented in 25th Annual Meeting SPGH, 2021.
  43. Gonçalves, Mariana. "Silva-Ribeiro CM*; Castro-Ribeiro C; Silva-Reis R; Gonçalves M; Bastos M.; Oliveira P. "Toxicological effects of pulegone and eugenol in laboratory animals: a pilot study". XII Jornadas de Genética e Biotecnologia, II Jornadas Ibéricas de Genetica y Biotecnología, University of Trás-os-Montes and Alto Douro *Presenting author". 2020.
  44. Gonçalves, Mariana. "Santos JI§*, Matos L§, Gonçalves M, Oliveira PA, Pires MJ, Coutinho MF, Prata MJ, Alves S. From bench to bioterium and back again: Development of a U1 snRNA-based therapeutic strategy for Mucopolysaccharidosis IIIC. 16th International Symposium of the Portuguese Society of Metabolic Disorders (SPDM). Online meeting. §These authors contributed equally to the work. *Presenting author". 2020.
  45. Gonçalves, Mariana. "Santos JI§, Matos L§*, Gonçalves M, Oliveira PA, Pires MJ, Coutinho MF, Prata MJ, Alves S. From bench to bioterium and back again: Development of a U1 snRNA-based therapeutic strategy for Mucopolysaccharidosis IIIC. 3rd International Conference on Sanfilippo Syndrome and Related Diseases. Sanfilippo Foundation Switzerland. Online meeting. §Equal contribution to the work. *Presenting author". 2020.
  46. Gonçalves, Mariana. "Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Development of an antisense oligonucleotide based therapy for Mucolipidosis type II a / ß: In vivo studies. 1º Expo Bioterios Virtual. Online Meeting. §These authors contributed equally to the work. *Presenting author". 2020.
  47. Gonçalves, Mariana. "Santos JI§*, Matos L§, Gonçalves M, Oliveira PA, Pires MJ, Coutinho MF, Prata MJ, Alves S. From bench to bioterium and back again: Development of a U1 snRNA-based therapeutic strategy for Mucopolysaccharidosis IIIC. Genetics Virtual Week 2020. Online Meeting. §These authors contributed equally to the work. *Presenting author". 2020.
  48. Gonçalves, Mariana. "Gonçalves M*, Ribeiro C, Reis R, Silva C, Matos L, Alves S, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Martins T, Oliveira P. Hydrodynamic injection in C57BL/6J mice: lateral effects. XII Jornadas de Genética e Biotecnologia. University of Trás-os-Montes and Alto Douro. Vila Real - Portugal. *Presenting author". 2020.
  49. Gonçalves, Mariana. "Matos L§, Santos JI§, Gonçalves M*, Oliveira PA, Pires MJ, Coutinho MF, Prata MJ, Alves S. From bench to bioterium and back again: Development of a U1 snRNA-based therapeutic strategy for Mucopolysaccharidosis IIIC. XII Jornadas de Genética e Biotecnologia. University of Trás-os-Montes and Alto Douro. Vila Real - Portugal. §These authors contributed equally to the work. *Presenting author". 2020.
  50. Gonçalves, Mariana. "Silva-Reis R*; Mendes B.; Castro-Ribeiro C; Gonçalves M; Ferreira T, Ribeiro-Silva CM;. Faustino-Rocha IA; Ginja MA ; Ferreira R; Oliveira P. "Monitoring of humane endpoints in an animal model of colorectal cancer. 1º Expobioterios virtual. *Presenting author,". 2020.
  51. Gonçalves, Mariana. "Silva-Reis R*; Castro-Ribeiro C; Gonçalves M; CM Ribeiro-Silva CM; Ferreira T; Gama A; Ferreira R; Oliveira P. "Cancro colorretal: comparação entre um bioensaio de médio e longo prazo num modelo animal". 2º Encontro Nacional de Jovens Investigadores em Oncologia, *Presenting author". 2020.
Journal article
  1. Gonçalves, Mariana; Santos, Juliana Inês; Coutinho, Maria Francisca; Matos, Liliana; Alves, Sandra. "Development of Engineered-U1 snRNA Therapies: Current Status". International Journal of Molecular Sciences 24 19 (2023): 14617. http://dx.doi.org/10.3390/ijms241914617.
    Published • 10.3390/ijms241914617
  2. Carvalho, Sofia; Santos, Juliana Inês; Moreira, Luciana; Gonçalves, Mariana; David, Hugo; Matos, Liliana; Encarnação, Marisa; Alves, Sandra; Coutinho, Maria Francisca. "Neurological Disease Modeling Using Pluripotent and Multipotent Stem Cells: A Key Step towards Understanding and Treating Mucopolysaccharidoses". Biomedicines 11 4 (2023): 1234. http://dx.doi.org/10.3390/biomedicines11041234.
    Published • 10.3390/biomedicines11041234
  3. Santos, Juliana Inês; Gonçalves, Mariana; Matos, Liliana; Moreira, Luciana; Carvalho, Sofia; Prata, Maria João; Coutinho, Maria Francisca; Alves, Sandra. "Splicing Modulation as a Promising Therapeutic Strategy for Lysosomal Storage Disorders: The Mucopolysaccharidoses Example". Life 12 5 (2022): 608. http://dx.doi.org/10.3390/life12050608.
    Published • 10.3390/life12050608
  4. Rita Silva-Reis; Catarina Castro-Ribeiro; Mariana Mendes Gonçalves; Tiago Ferreira; Maria João Pires; Carlos Eduardo Iglesias Aguirre; Adrián Cortés Martín; et al. "An Integrative Approach to Characterize the Early Phases of Dimethylhydrazine-Induced Colorectal Carcinogenesis in the Rat". Biomedicines (2022): https://www.mdpi.com/2227-9059/10/2/409.
    Published • 10.3390/biomedicines10020409
  5. Rita Silva-Reis; Ana I. Faustino-Rocha; Mariana Mendes Gonçalves; Catarina Castro-Ribeiro; Tiago Ferreira; Carla Ribeiro-Silva; LIO GONÇALVES; et al. "Refinement of Animal Model of Colorectal Carcinogenesis through the Definition of Novel Humane Endpoints". Animals (2021): https://www.mdpi.com/2076-2615/11/4/985.
    Published • 10.3390/ani11040985
  6. Basto, Sara ; Jorge, Sara ; Gonçalves, Mariana; Rema, Paulo. "Avaliação do efeito da frequência alimentar em juvenis de tenca (Tinca tinca) / [Evaluation of feeding frequency effect on tench (Tinca tinca) juveniles]". Revista Portuguesa de Zootecnia V 1 (2020): 54-68. https://www.apez.pt/index.php/rpz/artigos-in-press.
    Published
Thesis / Dissertation
  1. "Estudos de validação in vivo de abordagens terapêuticas de RNA para Doenças Lisossomais de Sobrecarga". Master, Universidade de Trás-os-Montes e Alto Douro, 2022.

Artistic / Performance

Video recording
  1. Medeiros-Fonseca, B; Paula Alexandra Martins de Oliveira; Ana Isabel Faustino-Rocha; Jéssica Silva; Tiago Terra Ferreira; Rita Silva-Reis; Gonçalves, Mariana; Elisabete Nascimento-Gonçalves. 2022. Qual a importância dos animais no progresso científico?.

Other

Other output
  1. Pre-neoplastic lesions associated with liver and colon responses to 1,2-dimethylhydrazine in an animal model of colorectal cancer. Background: The high incidence and mortality of colorectal cancer (CRC) combined with the lack of an effective method for early diagnosis and effective treatments make CRC one of the most relevant cancers to be studied. Thus, our work aims to study the spectrum of liver and colon lesions induced in rats by the 1,2-Dimethylhydrazine (DMH). Materials and methods: Twenty-nine male Wistar rats were ra. 2022. Silva-Reis, Rita; Faustino-Rocha, Ana Isabel; Castro-Ribeiro, Carla; Gonçalves, Mariana; Ferreira, Tiago; Gama, Adelina; Ferreira, Rita; Olveira, Paula Alexandra. http://hdl.handle.net/10174/30896.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/10 Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. RNA-Based therapies and disease models for Mucolipidosis II. Thematic Meeting of the Associated Laboratory AL4AnimalS - Comparative And Translational Medicine And Biotechnology ,Porto, Portugal 2023.*Presenting author.
(Porto, Portugal)
2023/05 Moreira L*, Coutinho MF, Duarte AJ, Gaspar P, Carvalho S, Santos JI, Gonçalves M, Matos L, Amaral O, Alves S. Generating gene-edited models for Lysosomal Storage Disorders. 2nd Meeting of the Associate Laboratory for Animal and Veterinary Sciences (AL4animalS), Vila Real - Portugal, May 2023. *Presenting author.
(Vila Real, Portugal)
2023/04 Carvalho S*, Santos JI, Moreira L, Gaspar P, Gonçalves M, Matos L, Encarnação, Ribeiro D, Duarte AJ, Prata MJ, Coutinho MF, Alves S. The Tooth Fairy that’s SHEDing hope for Rare Lysosomal Disorders. VI Center for the study of animal science (CECA) meeting. Casa de Vilar Porto Hotel, Porto – Portugal, April 2023. *Presenting author.
(Porto, Portugal)
2023/04 Moreira L*, Coutinho MF, Martinez O, Neuparth T, Ribeiro M, Pinho B, Oliveira N, Gaspar P, Carvalho S, Santos JI, Gonçalves M, Matos L, David H, Encarnação M, Oliveira J, Santos M, Alves S. Gene-edited zebrafish: a promising animal model for Lysosomal Storage Disorders and beyond. VI Center for the study of animal science (CECA) meeting. Casa de Vilar Porto Hotel, Porto – Portugal, April 2023. *Presenting author.
(Porto, Portugal)
2023/04 Santos JI*, Gonçalves M, Carvalho S, Matos L, Gaspar P, Prata MJ, Coutinho MF, Alves S. RNA as a promising molecule to treat a rare neurodegenerative lysosomal storage disorder. VI Center for the study of animal science (CECA) meeting. Casa de Vilar Porto Hotel, Porto – Portugal, April 2023. *Presenting author.
(Porto, Portugal)
2023/04 Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. Developing models and an RNA-based therapy for a rare disease. VI Center for the study of animal science (CECA) meeting. Casa de Vilar Porto Hotel, Porto – Portugal, April 2023. *Presenting author.
(Porto, Portugal)
2023/02 Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. Antisense oligonucleotide exon-skipping as a therapeutic approach for a rare disease. 53rd meeting of Portuguese Society of Farmacology (SPF), 41st Meeting of Clinical Pharmacology and 22nd Meeting of Toxicology, Coimbra– Portugal, February 2023. §These authors contributed equally to the work. *Presenting author.
(Coimbra, Portugal)
2022/11 Santos JI*, Gonçalves M, Matos L, Gaspar P, Pires MJ, Oliveira P, Prata MJ, Coutinho MF, Alves S. RNA as a promising molecule to treat a rare neurodegenerative lysosomal storage disorder. 3rd day of young researcher of National Health Institute Dr. Ricardo Jorge, Lisbon - Portugal, November 2022. *Presenting author.
(Lisboa, Portugal)
2022/11 Matos L§*, Gonçalves M§, Santos JI, Coutinho MF, Prata MJ, Omidi M, Pohl S, Alves S. A personalized antisense oligonucleotide exon-skipping therapeutic approach for Mucolipidosis II. Congress of Center for Interdisciplinary Research in Animal Health (CIISA) – Innovation in Animal, Veterinary and Biomedical Research, Lisbon – Portugal, November 2022. §These authors contributed equally to the work. *Presenting author.
(Lisboa, Portugal)
2022/11 Santos JI*, Gonçalves M, Matos L, Gaspar P, Pires MJ, Oliveira P, Prata MJ, Coutinho MF, Alves S. RNA as a promising molecule to treat a rare neurodegenerative lysosomal storage disorder. Congress of Center for Interdisciplinary Research in Animal Health (CIISA) – Innovation in Animal, Veterinary and Biomedical Research, Lisbon – Portugal, November 2022. *Presenting author.
(Lisboa, Portugal)
2022/11 Carvalho S*, Santos JI, Moreira L, Gaspar P, Gonçalves M, Matos L, Encarnação, Ribeiro D, Duarte AJ, Prata MJ, Coutinho MF, Alves S. Modelling genetic disorders with an unexpected co-worker: the Tooth Fairy that gifts hope to both children and scientists. Congress of Center for Interdisciplinary Research in Animal Health (CIISA) – Innovation in Animal, Veterinary and Biomedical Research, Lisbon – Portugal, November 2022. *Presenting author.
(Lisboa, Portugal)
2022/06 Carvalho S*, Santos JI, Ribeiro D, Moreira L, Duarte AJ, Encarnação M, Gaspar P, Gonçalves M, Matos L, Prata MJ, Pereira de Almeida L, Coutinho MF, Alves S. The 2020s Tooth Fairy: from loose tooth to neuronal cell cultures, an innovative method for in vitro genetic disease modeling of a rare neurological disorder. III International Meeting of the Portuguese Society of Genetics (SPG), Évora – Portugal, June 2022. *Presenting author.
(Évora, Portugal)
2022/02 Gonçalves M§*, Matos L§, Santos JI§, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. Mutation-adapted U1 snRNA as a therapeutic strategy for Mucopolysaccharidosis IIIC: in vitro and in vivo studies. 52nd meeting of Portuguese Society of Farmacology (SPF), 39th Meeting of Clinical Pharmacology and 21st Meeting of Toxicology, Porto, Portugal. §These authors contributed equally to the work. *Presenting author.
(Porto, Portugal)
2022/02 Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires MJ; et al. The influence of Ganoderma Lucidum un male mice fed with a western diet 39th Meeting of Clinical Pharmacology and 21st Meeting of Toxicology, Porto *Presenting author.
(Porto, Portugal)
2022/02 Silva-Reis R,. Faustino-Rocha I A, Castro-Ribeiro C, Gonçalves M, Ferreira T, Pires M J, Iglesias-Aguirre E C, Cortés-Martín A, Selma M V, Espín C J; Nascimento-Gonçalves E, Moreira-Pais A, Neuparth M J, Peixoto F, Rosa E, Gama A, Ferreira R, Oliveira P. The effects of dimethylhydrazine intraperitoneal administration: an integrative approach to understand its effects 52nd meeting of Portuguese Society of Farmacology (SPF), 39th Meeting of Clinical Pharmacology and 21st Meeting of Toxicology, Porto – Portugal *Presenting author
(Porto, Portugal)
2021/10 Gonçalves M*., Santos JI., Matos L., Oliveira PA., Pires MJ., Coutinho MF., Prata MJ., Alves S. In vivo validation studies of RNA therapeutic approaches for Lysosomal storage diseases ". Seminários do departamento de genética humana, Instituto Nacional de Saúde Dr. Ricardo Jorge, *Presenting author
(Porto, Portugal)
2021/10 Matos L*, Coutinho MF, Santos JI, Gonçalves M, Duarte AJ, Ribeiro D, Moreira L, Encarnação M, Gaspar P, Amaral O, Carvalho S, Alves S. RNA como alvo terapêutico: desenvolvimento de abordagens inovadoras utilizando oligonucleótidos sintéticos. V Jornadas Científicas do Centro de Estudos de Ciência Animal (CECA). Instituto de Ciências Biomédicas de Abel Salazar *Presenting author
(Porto, Portugal)
2021/10 Matos L*, Gonçalves M, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. E se a retirada de um exão pudesse ter um efeito terapêutico? Caminhos de uma abordagem terapêutica molecular para uma doença rara através de exon-skipping. V Jornadas Científicas do Centro de Estudos de Ciência Animal (CECA). Instituto de Ciências Biomédicas de Abel Salazar *Presenting author
(Porto, Portugal)
2021/10 Coutinho MF *, Santos JI, Carvalho S, Gaspar P, Gonçalves M, Matos L, Encarnação M, Ribeiro D, Duarte AJ, Amaral O, Rocha H, Prata MJ, Alves S. Uma fada minúscula e um peixe tropical: heróis improváveis na luta contra uma doença rara. V Jornadas Científicas do Centro de Estudos de Ciência Animal (CECA). Instituto de Ciências Biomédicas de Abel Salazar (ICBAS) *Presenting author.
(Porto, Portugal)
2021/09 Santos JI *, Gonçalves M, Matos L, Gaspar P, Pires MJ, Oliveira P, Prata MJ, Coutinho MF, Alves S. Targeted RNA-based therapies for Mucopolysaccharidosis. 17th International Symposium of the Portuguese Society of Metabolic Disorders (SPDM). *Presenting author
(Fátima , Portugal)
2021/04 Gonçalves M§*, Matos L§, Santos JI§, Coutinho MF, Prata MJ, Pires MJ, Oliveira PA, Alves S. Mutation-adapted U1 snRNA as a therapeutic strategy for Mucopolysaccharidosis IIIC: in vitro and in vivo studies. XIII Jornadas de Genética e Biotecnologia / III Jornadas Ibéricas de Genética y Biotecnología. Online meeting. §These authors contributed equally to the work. *Presenting author.
(León, Spain)
2021/03 Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Antisense oligonucleotide exon-skipping as a therapeutic approach for Mucolipidosis type II a/ß: in vitro and in vivo studies. Jornadas de Ciência. University of Trás-os-Montes and Alto Douro. §These authors contributed equally to the work. *Presenting author
(Vila Real , Portugal)
2021/03 Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; Taofiq O; Barros L; Martins T; Pires MJ; et al. Glucose tolerance in C57Bl/6J mice supplemented with Ganoderma lucidum Jornadas de Ciência. University of Trás-os-Montes and Alto Douro. *Presenting author
(Vila Real , Portugal)
2021/02 Gonçalves M§*, Matos L§, Santos JI, Coutinho MF, Prata MJ, Pires MJ, Oliveira P, Alves S. Development of an antisense oligonucleotide based therapy for Mucolipidosis type II a/ß: in vivo studies. 51st meeting of SPF (Portuguese Society of Farmacology), 38th Meeting of Clinical Pharmacology and 20th Meeting of Toxicology Faculty of Pharmacy of the University of Lisbon. §These authors contributed equally to the work. *Presenting author.
(Lisboa , Portugal)
2021/02 Medeiros-Fonseca B*; Gonçalves M; Silva-Reis R; Peixoto F; Gaivão I; Matos M; Pinto M ; Roboredo M; Ricardo J; Coimbra AC; Oliveira P. EISENIA FETIDA: OPTIMIZATION FOR PHARMACOLOGICAL/TOXICOLOGICAL ASSAYS 38th Meeting of Clinical Pharmacology and 20th Meeting of Toxicology Faculty of Pharmacy of the University of Lisbon. *Presenting author
(Lisboa , Portugal)
2021/02 Silva-Reis R*; Ribeiro C; Gonçalves M; Ferreira, T; Pires M J; Gama A; Ferreira R; Oliveira P. A Characterization of colorectal carcinogenesis in a rat animal model 38th Meeting of Clinical Pharmacology and 20th Meeting of Toxicology Faculty of Pharmacy of the University of Lisbon. *Presenting author
(Lisboa , Portugal)
2021/02 Castro-Ribeiro C*; Silva-Reis R; Gonçalves M; O. Taofiq; L. Barros; T. Martins; M.J. Pires; et al. The influence of ganoderma lucidum in the liver of an animal model of obesity 38th Meeting of Clinical Pharmacology and 20th Meeting of Toxicology, Faculty of Pharmacy of the University of Lisbon. *Presenting author
(Lisboa , Portugal)
2020/02 Gonçalves M*, Ferreira T, Rodrigues P, Nascimento-Gonçalves E, Gil-da-Costa M R, Martins T, Neuparhf MJ, Pires MJ, Lanzarina G, Félix L, Venâncio C ,Ferreira I, Bastos M, Medeiros R, Gaivão I, Rosa E , Oliveira P. Diet supplemented with 1.1% chestnut flour reduces abdominal adiposity in mice. 37th Meeting of Clinical Pharmacology and 19 th Meeting of Toxicology *Presenting author
(Coimbra , Portugal)
2020/02 Castro-Ribeiro C *, Silva-Reis R, Gonçalves, Ferreira T, Martins T, Antunes L, Venâncio C, Gonçalves L, Oliveira P Body temperature determination in rats by infrared: using two different equipments 37th Meeting of Clinical Pharmacology and 19 th Meeting of Toxicology *Presenting author
(Coimbra , Portugal)
2020/02 Silva-Reis R*, Castro-Ribeiro C, Ribeiro-Silva CM, Gonçalves M, Ferreira T, Pires MJ, Ferreira R, Gama A, Faustino-Rocha A I, Venâncio C, Oliveira P. Morphometric analysis of rat parameters during an animal model of colorectal cancer. XII Jornadas de Genética e Biotecnologia, II Jornadas Ibéricas de Genetica y Biotecnología, Universidade de Trás-os-Montes e Alto Douro *Presenting author.
(Vila Real , Portugal)
2020 Silva-Reis R, Gonçalves M , Catarina Castro-Ribeiro, Ferreira T , Ginja M, Ferreira R, Oliveira P Establishment of humane endpoints in an animal model of colorectal cancer in male wistar rats. 37th Meeting of Clinical Pharmacology and 19 th Meeting of Toxicology *Presenting author.
(Coimbra , Portugal)

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2023 - Current VI CECA Meeting (2023/03/31 - 2023/04/01)
Congress (Member of the Organising Committee)
 Universidade do Porto, Portugal
2017 - 2017 X JORNADAS DE BIOLOGIA (2017 - 2017)
Congress (Member of the Organising Committee)
 Universidade de Trás-os-Montes e Alto Douro, Portugal
2017 - 2017 CAMPUS DO MAR, INTERNATIONAL SCIENCE CONFERENCE (2017 - 2017)
Congress (Member of the Organising Committee)
 Universidade de Aveiro, Portugal

Universidade de Trás-os-Montes e Alto Douro, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2019 - 2019 Seminar in the context of the Master in Clinical and Laboratory Biology
Seminar
 3º Seminar in Immunology: "QUANDO A DEFESA É O ATAQUE"
Universidade de Trás-os-Montes e Alto Douro, Portugal
2019 - 2019 Scientific and dynamic approach to share knowledge, debate and reflect on the challenges of Experimental Pharmacology
Other
 Pharmacology research forum: "UMA ÚNICA SAÚDE"
Universidade de Trás-os-Montes e Alto Douro, Portugal
2017 - 2017 Annual scientific event that aims to update knowledge in the area of Biology
Meeting
 National meeting of Biology students
Universidade de Aveiro, Portugal
2017 - 2017 This workshop aims to perform the baptism of diving offering the opportunity to see various aquatic organisms
Workshop
 "INICIAÇÃO AO MERGULHO"
Universidade de Aveiro, Portugal
2017 - 2017 Scientific and dynamic approach to share knowledge, debate and reflect on the area of biology VIII JORNADAS DE BIOLOGIA
Universidade de Trás-os-Montes e Alto Douro, Portugal
2016 - 2016 Annual scientific event that aims to update knowledge in the area of Biology
Meeting
 National meeting of Biology students
Universidade de Coimbra, Portugal
2016 - 2016 This workshop aims to explore the area of Forensic Science in which insect information is used in criminal investigation.
Workshop
 "ENTOMOLOGIA FORENSE"
Universidade de Coimbra, Portugal
2016 - 2016 Scientific event aimed at better knowledge of wild and exotic animals
Symposium
 V SYMPOSIUM OF WILD AND EXOTIC
Universidade de Trás-os-Montes e Alto Douro, Portugal
2016 - 2016 Annual scientific event that aims to share knowledge, debate and reflect on the different branches of bioethics
Meeting
 II ENCONTRO DE BIOÉTICA
Universidade de Trás-os-Montes e Alto Douro, Portugal
2015 - 2015 Annual scientific event that aims to update knowledge in the area of Biology
Meeting
 National meeting of Biology students
Universidade do Minho Departamento de Biologia, Portugal
2015 - 2015 This workshop aims to explore the role of mycorrhizal roots in soil
Workshop
 "À PROCURA DE RAÍZES MICORRIZADAS”
Universidade do Minho Departamento de Biologia, Portugal
Distinctions

Award

2022 Best conference Poster, Dia do Jovem Investigador
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2021 Best Oral Communication, 17th Portuguese Society of Metabolic Disorders (SPDM)