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Joana Valente started her scientific path working with polymers, 3DP, materials characterization and drug delivery systems (DDS) development. Then, she went to work on a FCT project and gained expertise with affinity chromatography and plasmid DNA (pDNA) purification as well as with techniques of analysis of pDNA purity. During this time she started her PhD with an individual FCT grant to work with commercial and novel chromatographic matrices, to accomplish the recovery of the supercoiled (sc) isoform of different pDNA. She established partnerships with international companies and she did an internship in UCBIO-FCT which lead her to acquire unevaluable knowledge in ligands immobilization field. As a postdoc researcher (2019), she is developing different drug carriers and antimicrobial materials and, she is pursuing the development of more efficient chromatographic supports conventionally produced or using AM methodologies. She was the principal investigator (PI) of the project coPOLY (298.320,48€) and, she is a team member of another 3 projects funded by ANI (ongoing). She is responsible for 2 laboratories at CDRSP, reviewer of different international journals, guest editor on Pharmaceutics, part of the conference organizing committee, part of the CDRSP scientific council and academic jurys. Main scientific achievements: several publications in peer review journals (as first and last author as well as part of the team); a patent related AM with chromatography; promoting the industry (national and international companies) interest in the different works that I am developing; ability to catch funding (PI and part of the team); beginning of her scientific team.
Identification

Personal identification

Full name
Joana Valente

Citation names

  • J.F.A. Valente

Author identifiers

Ciência ID
7515-7468-212D
ORCID iD
0000-0002-2408-9762
Google Scholar ID
VABrK9AAAAAJ

Email addresses

  • joana.valente@ipleiria.pt (Professional)

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Biomaterials
  • Engineering and Technology - Materials Engineering
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
  • Engineering and Technology - Nano-technology - Nano-materials
  • Engineering and Technology - Industrial Biotechnology - Industrial Biotechnology
  • Engineering and Technology - Industrial Biotechnology - Bioproducts Biomaterials

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2013/09 - 2018/11
Concluded
Biochemistry (Doutoramento)
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Affinity purification and cell delivery of a p53-encoding plasmid DNA for gene therapy " (THESIS/DISSERTATION)
Excelent
2018/01/11
Concluded
Good Manufatcuring Practices na Indústria Farmacêutica: como transformar regras em ferramentas de produtividade (Outros)
Universidade da Beira Interior, Portugal
2015 - 2015
Concluded
Auditor training course: Integrated management Systems (Quality, Environment and Safety) (Diploma de especialização)
Academia da SGS, Portugal
2015
Concluded
Post Graduated Course in Integrated Management Systems (Environment, Quality, Safety and Social Responsibility ) (Pós-Graduação)
Instituto Politécnico da Guarda, Portugal
"n/a" (THESIS/DISSERTATION)
15
2013/11/22 - 2013/11/24
Concluded
I Hands-on course on fluorescence microscopy and image analysis (Outros)
Universidade da Beira Interior, Portugal
2011
Concluded
Engineering for product conception and development (Mestrado)
Instituto Politécnico de Leiria Escola Superior de Tecnologia e Gestão, Portugal
"Surface modification of alginate scaffolds" (THESIS/DISSERTATION)
19
2008
Concluded
Biomechanics (Licenciatura)
Instituto Politécnico de Leiria Escola Superior de Tecnologia e Gestão, Portugal
"Palmilhas para diabéticos" (THESIS/DISSERTATION)
14
Affiliation

Science

Category
Host institution
Employer
2022/01/01 - Current Researcher (Research) Instituto Politécnico de Leiria, Portugal
2019/01/28 - Current Postdoc (Research) Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal

Teaching in Higher Education

Category
Host institution
Employer
2014/09 - 2017/06 Assistant (University Teacher) Universidade da Beira Interior, Portugal

Others

Category
Host institution
Employer
2021/06 - Current CO-HEAD OF THE CELL CULTURE LABORATORY Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal
2021/06 - Current CO-HEAD OF THE SYNTHESIS LABORATORY Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal
2014/09/01 - 2018/09/01 FCT Project with reference : SFRH/BD/96809/2013 Thesis project entitled: "Affinity purification and cell-specific delivery of a p53-encoding plasmid DNA for gene therapy" Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
2016 - 2018 CO-HEAD OF THE CENTRIFUGE LABORATORY Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012/09/03 - 2014/02/28 Researcher in the context of the FCT Project with reference "PTDC/EBB-BIO/114320/2009" entitled "Isolation and purification of plasmid DNA for cancer therapy ” Universidade da Beira Interior, Portugal
2011/03/01 - 2012/09/01 Researcher in the context of the FCT Project with reference PTDC/EME-TME/103375/2008 entitled :"Production of scaffolds improved with growth factors for bone tissue regeneration” Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2009/03/23 - 2010/03/23 Researcher in the project: Rapid production of structures for drug delivery Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal
Projects

Grant

Designation Funders
2021 - Current miniGEN4kill - Biotechnological platform based on gene therapy with minicircle DNA and antiviral nanoparticles to treat and fight cervical cancer
2021.09331.BD
Supervisor
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
2019 - 2020/12 MATIS - Materials and Sustainable Industrial Technologies
CENTRO-01-0145-FEDER-000014 - 3362
Horizon 2020 Framework Programme
2014 - 2018/08 Affinity purification and cell delivery of a p53-encoding plasmid DNA for gene therapy
SFRH/BD/96809/2013
Master Student Fellow
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2012 - 2014/08 Isolation and Purification of Plasmid DNA for Cancer Therapy
PTDC/EBB-BIO/114320/2009
Master Student Fellow
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011 - 2012/08 Production of scaffolds improved with growth factors for bone tissue regeneration
PTDC/EME-TME/103375/2008
Master Student Fellow
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Concluded
2009 - 2010 “3D Printing scaffolds with growth factors for bone tissue regeneration”
IT
Scientific Initiation Fellow
Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal

Contract

Designation Funders
2022 - Current Bone2Move
POCI-01-0145-FEDER-031146
Researcher
Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal
Fundação para a Ciência e a Tecnologia

Other

Designation Funders
2021 - Current BioRobotBeads- Robotic system for biomaterials and particles production
Researcher
Agência Nacional de Inovação SA
2022 - 2025/12 BioShoes4All
PRR- Bioeconomia sustentável
Researcher
Agência Nacional de Inovação SA
Ongoing
2022 - 2025 Inovação na Fileira da Resina Natural para Reforço da Bioeconomia Nacional
PRR- Bioeconomia sustentável
Researcher
Agência Nacional de Inovação SA
Ongoing
2020 - 2020/11/30 coPOLY- Polymers reinforced with copper to promote antimicrobial effect
Centro-01-02B7-FEDER-069244
Principal investigator
Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal
Agência Nacional de Inovação SA
Ongoing
2017 - 2017/04/01 A library of amino acids to screen adsorbents suitable for sc p53 encoding plasmid isolation
SFRH/BD/96809/2013
PhD Student Fellow
UCBIO-FCT, Portugal
Concluded
Outputs

Publications

Book chapter
  1. A.R. Carapito; J.F.A. Valente; J.A. Queiroz; F. sousa. "Arginine-affinity chromatography for nucleic acids (DNA and RNA) isolation". In Affinity Chromatography. Springer, 2021.
    Accepted
Conference abstract
  1. Ana Catarina Sousa; Biscaia, Sara; Alvites, Rui Damásio; Mariana Branquinho; Lopes, Bruna; Patrícia Sousa; Valente, Joana F.A.; et al. "Production, characterisation, and in vitro evaluation of 3D printed PCL/HANp/PEGDA scaffold for bone regeneration". Paper presented in MATERIAIS2022, 2022.
  2. Pereira, Diana; Susana ferreira; ALVES, NUNO; ângela sousa; Joana F. A. Valente. "Chitosan-Silver Nanoconjugates to Achieve Enhanced Antimicrobial Effect and Fight Cervical Cancer". Paper presented in Materials 2022, Marinha Grande, 2022.
    Accepted
  3. Pereira, Diana; Susana Ferreira; ALVES, NUNO; Ângela Sousa; Valente, Joana F.A.. "Assessment of the Antimicrobial Effect of Dopamine-Coated Silver Nanoparticles for Therapeutic Applications". Paper presented in Materials 2022, Marinha Grande, 2022.
    Accepted
  4. Daniela Carvalho; Pereira, Diana; ALVES, NUNO; Andreas Pickl; Sara Cardoso; Valente, Joana F.A.; Ângela Sousa. "New Combined Approach to Simplify the Minicircular DNA Lysate and Directly Apply in Ion Exchange Membrane Chromatography". Paper presented in Materials 2022, Marinha Grande, 2022.
    Accepted
  5. Valente, Joana F.A.; Juliana R. Dias; Fani Sousa; ALVES, NUNO. "3D printing for affinity chromatographic support production". Paper presented in Materials 2022, Marinha Grande, 2022.
    Accepted
  6. Santos, Cyril; Hugo Marques; Mateus, Artur; ALVES, NUNO; J.F.A. Valente. "Polymeric blends with copper: a powerful weapon against pathogenic spread". Paper presented in Materials 2022, Marinha Grande, 2022.
    Accepted
  7. S.F.C. Guerreiro; C.A.M. Ferreira; J.F.A. Valente; N. Alves; J.R. Dias. "SpinningTNT: development of bioactive TNT by electrospinning". Paper presented in 4th Edition of the International Conference on Direct Digital Manufacturing and Polymers, Marinha Grande, 2021.
  8. S.F.C. Guerreiro; Juliana R. Dias; J.F.A. Valente; N. Alves. "A Design of Experiment methodology to study the influence of production conditions in the behaviour of PCL/gelatin electrospun skin wound dressings". Paper presented in 31st Conference of the European Society for Biomaterials, 2021.
Conference poster
  1. Pereira, Diana; Susana Ferreira; ALVES, NUNO; Ângela Sousa; Valente, Joana F.A.. "Chitosan-Silver Nanoconjugates to Achieve Enhanced Antimicrobial Effect and Fight Cervical Cancer". Paper presented in Materials 2022, 2022.
  2. Pereira, Diana; Susana Ferreira; ALVES, NUNO; Ângela Sousa; Valente, Joana F.A.. "Assessment of the Antimicrobial Effect of Dopamine-Coated Silver Nanoparticles for Therapeutic Applications". Paper presented in Materials 2022, 2022.
  3. Daniela Carvalho; Pereira, Diana; ALVES, NUNO; Andreas Pickl; Sara Cardoso; Valente, Joana F.A.; Ângela Sousa. "New Combined Approach to Simplify the Minicircular DNA Lysate and Directly Apply in Ion Exchange Membrane Chro-matography". Paper presented in Materials 2022, 2022.
  4. Valente, Joana F.A.; Juliana R. Dias; Fani Sousa; ALVES, NUNO. "3D printing for affinity chromatographic support production". Paper presented in Materials 2022, 2022.
  5. Valente, Joana F.A.; Juliana R. Dias; Fani Sousa; ALVES, NUNO. "Polymeric blends with copper: a powerful weapon against pathogenic spread". Paper presented in Materials 2022, 2022.
  6. Santos, Cyril; Hugo Marques; Mateus, Artur; ALVES, NUNO; J.F.A. Valente. "Polymeric blends with copper: a powerful weapon against pathogenic spread". Paper presented in Materials 2022, 2022.
  7. Ana Catarina Sousa; Biscaia, Sara; Rui Alvites; Mariana Branquinho; Bruna Lopes; Patrícia Sousa; Valente, Joana F.A.; et al. "Production, characterisation, and in vitro evaluation of 3D printed PCL/HANp/PEGDA scaffold for bone regeneration.". Paper presented in MATERIAIS2022, 2022.
  8. D. Pereira; N. Alves; A. Sousa; J.F.A. Valente. "Modified Silver Nanoparticles for Cervical Cancer Therapy". Paper presented in XVI Annual CICS-UBI Symposium, 2021.
  9. J.F.A. Valente; C. Santos; H. Marques; A. Mateus; N. Alves. "coPOLY - Polymers reinforced with copper to promote antimicrobial effect". Paper presented in 4th Edition of the International Conference on Direct Digital Manufacturing and Polymers, 2021.
  10. S.F.C. Guerreiro; J.R. Dias; J.F.A. Valente; N. Alves. "A Design of Experiment methodology to study the influence of production conditions in the behaviour of PCL/gelatin electrospun skin wound dressings". Paper presented in 31st Conference of the European Society for Biomaterials, 2021.
  11. J.F.A. Valente. "Plasmid DNA nano-complexation with Polyethyleneimine - an alternative to commercial nanovehicles". Paper presented in 11th World Biomaterials Congress, 2020.
  12. J.F.A. Valente; P. Pereira; A. Sousa; J.A. Queiroz; F. Sousa. "Plasmid DNA nano-complexation with Polyethyleneimine - an alternative to commercial nanovehicles". Paper presented in 11th World Biomaterials Congress, 2020.
  13. Pinto, J.M.; Valente, J.F.A.; Pereira, P.; Pina, A.S.; Queiroz, J.A.; Roque, A.C.A.; Sousa, F.. "Could it be possible to quickly determine the best ligands?". Paper presented in II International Congress in Health Sciences Research: Towards Innovation and entrepreneurship, 2017.
  14. Pinto, J.M.; Figueiró, G.A.; Valente, J.F.A.; Pina, A.S.; Pereira, P.; Queiroz, J.A.; Roque, A.C.A.; Sousa, F.. "Recovery of recombinant microRNAs with modified magnetic nanoparticles". Paper presented in XII ANNUAL CICS-UBI SYMPOSIUM, 2017.
  15. Valente, J.F.A.; Sousa, A.; Queiroz, J.A.; Sousa, F.. "Supercoiled p53-encoding plasmid purification by amino acids-based affinity chromatography – A comparison". Paper presented in 9º ENCONTRO NACIONAL DE CROMATOGRAFIA / XVI COLACRO, 2016.
  16. Valente, J.F.A.; Sousa, A.; Cruz, C.; Queiroz, J.A.; Sousa, F.. "Novel superporous arginine matrix for supercoiled p53 encoding plasmid isolation". Paper presented in Microbiotec, 2015.
  17. Valente, J.F.A.; Sousa, A.; Queiroz, J.A.; Sousa, F.. "Purification of the supercoiled p53-encoding plasmid by a L-methionine-agarose matrix". Paper presented in 10th ESBES-IFIBiop, 2014.
  18. Valente, J.F.A.; Correia, I.J.; Sousa, F.. "p53 Supercoiled plasmid purification by l-methionine agarose matrix". Paper presented in 19th International Symposium on Separation Sciences, 2013.
Journal article
  1. Diana Pereira; Tiago Soares Carreira; Nuno Alves; Ângela Sousa; Joana F. A. Valente. Corresponding author: Joana F. A. Valente. "Metallic Structures: Effective Agents to Fight Pathogenic Microorganisms". International Journal of Molecular Sciences 23 3 (2022): 1165-1165. https://doi.org/10.3390/ijms23031165.
    10.3390/ijms23031165
  2. Sousa, Ângela; Almeida, Ana Margarida; Valente, Joana; Queiroz, João; Sousa, Fani. "Hands-On Laboratory Class for Biopharmaceutical pDNA Quality Control". Journal of Chemical Education 99 2 (2022): 975-982. http://dx.doi.org/10.1021/acs.jchemed.1c00695.
  3. J.F.A. Valente; F. Sousa; N. Alves. Corresponding author: J.F.A. Valente. "Additive Manufacturing Tools to Improve the Performance of Chromatographic Approaches". Trends in Biotechnology (2021): https://doi.org/10.1016/j.tibtech.2021.03.008.
    10.1016/j.tibtech.2021.03.008
  4. Valente, J.F.A.; Pereira, P.; Sousa, A.; Queiroz, J.A.; Sousa, F.. Corresponding author: Valente, J.F.A.. "Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation". Polymers 13 5 (2021): 793. http://dx.doi.org/10.3390/polym13050793.
    10.3390/polym13050793
  5. Valente, J.F.A.; Queiroz, J.A.; Sousa, F.. "Dilemma on plasmid DNA purification: binding capacity vs selectivity". Journal of Chromatography A 1637 (2021): 461848. http://dx.doi.org/10.1016/j.chroma.2020.461848.
    Accepted • 10.1016/j.chroma.2020.461848
  6. Sara F. C. Guerreiro; J.F.A. Valente; Juliana R. Dias; NUNO ALVES. "Box-Behnken Design a Key Tool to Achieved Optimized PCL/Gelatin Electrospun Mesh". Macromolecular Materials and Engineering (2021):
    Accepted
  7. J.F.A. Valente; A. Sousa; G.A. Azevedo; J.A. Queiroz; F. Sousa. "Purification of supercoiled p53-encoding plasmid using an arginine-modified macroporous support". Journal of Chromatography A (2020): https://doi.org/10.1016/j.chroma.2020.460890.
    10.1016/j.chroma.2020.460890
  8. Azevedo, G.M.; Valente, J.F.A.; Sousa, A.; Pedro, A.Q.; Pereira, P.; Sousa, F.; Queiroz, J.A.. "Effect of Chromatographic Conditions on Supercoiled Plasmid DNA Stability and Bioactivity". Applied Sciences 9 23 (2019): 5170. http://dx.doi.org/10.3390/app9235170.
    10.3390/app9235170
  9. J.F.A. Valente; J.R. Dias; A. Sousa; N. Alves. Corresponding author: J.F.A. Valente. "Composite Central Face Design—An Approach to Achieve Efficient Alginate Microcarriers". Polymers 11 12 (2019): 1949-1949. https://doi.org/10.3390/polym11121949.
    10.3390/polym11121949
  10. Valente, J.F.A.; Sousa, A.; Queiroz, J.A.; Sousa, F.. "DoE to improve supercoiled p53-pDNA purification by O-phospho-l-tyrosine chromatography". Journal of Chromatography B 1105 (2019): 184-192. http://dx.doi.org/10.1016/j.jchromb.2018.12.002.
    Published • 10.1016/j.jchromb.2018.12.002
  11. Valente, J.F.A.; Sousa, A.; Gaspar, V.M.; Queiroz, J.A.; Sousa, F.. "The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines". Process Biochemistry 75 (2018): 240-249. http://dx.doi.org/10.1016/j.procbio.2018.09.014.
    Published • 10.1016/j.procbio.2018.09.014
  12. Valente, Joana F.A.; Queiroz, João A.; Sousa, Fani. "p53 as the Focus of Gene Therapy: Past, Present and Future". Current Drug Targets 19 15 (2018): 1801-1817. http://dx.doi.org/10.2174/1389450119666180115165447.
    Published • 10.2174/1389450119666180115165447
  13. Ferreira, Soraia; Carvalho, Josué; Valente, Joana F.A.; Corvo, Marta C.; Cabrita, Eurico J.; Sousa, Fani; Queiroz, João A.; Cruz, Carla. "Affinity analysis and application of dipeptides derived from l -tyrosine in plasmid purification". Journal of Chromatography B 1006 (2015): 47-58. http://dx.doi.org/10.1016/j.jchromb.2015.10.025.
    10.1016/j.jchromb.2015.10.025
  14. Valente, J.F.A.; Sousa, A.; Queiroz, J.A.; Sousa, F.. "Selective purification of supercoiled p53-encoding pDNA with l-methionine–agarose matrix". Analytical Biochemistry 459 (2014): 61-69. http://dx.doi.org/10.1016/j.ab.2014.05.011.
    10.1016/j.ab.2014.05.011
  15. Fonseca, Ana C.; Coelho, Jorge F.J.; Valente, Joana F.A.; Correia, Tiago R.; Correia, Ilídio J.; Gil, Maria H.; Simões, Pedro N.. "Poly(ester amide)s based on (L)-lactic acid oligomers and a-amino acids: influence of the a-amino acid side chain in the poly(ester amide)s properties". Journal of Biomaterials Science, Polymer Edition 24 12 (2013): 1391-1409. http://dx.doi.org/10.1080/09205063.2012.762293.
    Published • 10.1080/09205063.2012.762293
  16. Valente, J.F.A.; Gaspar, V.M.; Antunes, B.P.; Countinho, P.; Correia, I.J.. "Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration". Polymer 54 1 (2013): 5-15. http://dx.doi.org/10.1016/j.polymer.2012.10.032.
    Published • 10.1016/j.polymer.2012.10.032
  17. Valente, J.F.A.; Valente, T.A.M.; Alves, P.; Ferreira, P.; Silva, A.; Correia, I.J.; Valente, T.. "Alginate based scaffolds for bone tissue engineering". Materials Science and Engineering C 32 8 (2012): 2596-2603. http://www.scopus.com/inward/record.url?eid=2-s2.0-84866034437&partnerID=MN8TOARS.
    Published • 10.1016/j.msec.2012.08.001
Journal issue
  1. J.F.A. Valente; F. sousa. "Progress and Innovation on Nanosystems for gene therapy". Pharmaceutics (2022):
    Accepted • Editor
  2. J.F.A. Valente; ALVES, NUNO. "Additive Manufacturing Approaches to Produce Drug Delivery Systems Volume II". Pharmaceutics (2022):
    Accepted • Editor
  3. J.F.A. Valente; NUNO ALVES. "Additive Manufacturing Approaches to Produce Drug Delivery Systems". Pharmaceutics (2021):
    Published • Editor
Thesis / Dissertation
  1. Valente, Joana. "Affinity purification and delivery of a p53-encoding plasmid DNA for gene mediated cancer therapy". PhD, Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, 2018. http://hdl.handle.net/10400.6/6602.
  2. Valente, Joana. "Surface modification of alginate scaffolds". Master, Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, 2011. http://hdl.handle.net/10400.6/6602.

Intellectual property

Patent
  1. J.F.A. Valente. 2020. "DISPOSITIVO PARA SEPARAÇÃO E QUANTIFICAÇÃO DE DIFERENTES COMPOSTOS". Portugal.
    Granted/Issued
  2. Valente, Joana. 2011. "Dispositivo de auxílio a invisuais".
    Allowed
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/06 Affinity chromatographic strategies to recover the supercoiled p53-encoding plasmid Chemeet- International Chemistry Conference
2021/02/04 coPOLY - Polymers reinforced with copper to promote antimicrobial effect International Manufacturing Forum Series 2021
(Virtual)
2021 Additive manufacturing: a bright future for chromatographic supports production 4th Edition of the International Conference on Direct Digital Manufacturing and Polymers
(Marinha Grande, Portugal)
2017 A library of amino acids to screen adsorbents suitable for sc p53 encoding plasmid isolation II International Congress in Health Sciences Research: Towards Innovation and entrepreneurship
(Co, Portugal)
2017 Purification and delivery of the p53 pDNA genome guardian XII ANNUAL CICS-UBI SYMPOSIUM
(Covilhã, Portugal)
2016 p53 supercoiled encoding plasmid in different cancer cell lines – Transfection and Expression effects Bio.Iberoamerica
(Salamanca, Spain)
2016 Superporous affinity chromatography for the supercoiled p53 encoding plasmid isolation ISPPP
(Salzburg, Austria)
2014 Selective purification of supercoiled p53-encoding pDNA with L-methionine-agarose matrix IX ANNUAL CICS-UBI SYMPOSIUM
(Covilhã, Portugal)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2021/09/01 - Current miniGEN4kill - Biotechnological platform based on gene therapy with minicircle DNA and antiviral nanoparticles to treat and fight cervical cancer
Co-supervisor
Biomedicina (PhD)
Universidade da Beira Interior, Portugal
2021 - Current Application of membranes for clarification and/or purification of a minicircular DNA vector
Co-supervisor
Biotecnologia (Master)
Universidade da Beira Interior, Portugal
2022/03 - 2022/05 Silver nanoparticles: Structures applied to cancer diagnosis and treatment
Co-supervisor
Bioquímica (Degree)
Universidade da Beira Interior, Portugal
2022/03 - 2022/05 Therapeutic strategy against cervical cancer based using metallic particles
Co-supervisor
Biotecnologia (Degree)
Universidade da Beira Interior, Portugal
2022/03 - 2022/05 Targeted delivery of the p53 supercoiled gene using chitosan nanoparticles
Supervisor
Bioquímica (Degree)
Universidade da Beira Interior, Portugal
2021 - 2021 3D printed structures immobilized with different amino acids as plasmid DNA absorbers
Supervisor
Biotecnologia (Degree)
Instituto Politécnico de Leiria, Portugal
2021 - 2021 Adsorption behaviour of arginine 3D printed structures using different nucleic acids
Supervisor
Biotecnologia (Degree)
Instituto Politécnico de Leiria, Portugal
2020 - 2020 Biotechnological platform to produce p53 encoding pDNA
Supervisor
Biotecnologia (Degree)
Instituto Politécnico de Leiria Escola Superior de Turismo e Tecnologia do Mar de Peniche, Portugal

Event organisation

Event name
Type of event (Role)
Institution / Organization
2022/04/10 - 2022/04/13 Materiais 2022 (2022/04/10 - 2022/04/13)
Conference (Member of the Organising Committee)
Sociedade Portuguesa de Materiais, Portugal

Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal
2013/12/02 - 2013/12/04 8º Encontro Nacional de Cromatografia (2013/12/02 - 2013/12/04)
Congress (Co-organisor)
Universidade da Beira Interior, Portugal

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2022 Optimization of chitosan-based nanosystems to deliver plasmid DNA vaccines
(Thesis) Main arguer
Carlos Afonso Rodolfo (Master)
Universidade da Beira Interior, Portugal
2021 Adaptação de uma vacina de DNA contra as variantes do SARSCoV2 Adaptation of a DNA vaccine against SARSCoV2 variants
(Thesis) Main arguer
Vasco Miguel Leite Ferreira Margarido (Other)
Universidade da Beira Interior, Portugal
2020/01/06 Evaluation of the multigenic minicircular DNA vector action on cervical cancer cells
(Thesis) Main arguer
Diana Carvalho Pereira (Master)
Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
2016 Binding mechanism of monoclonal antibodies to protein A
(Thesis) Main arguer
Ana Sofia Ferreira Alves Marques da Silva (Other)
Universidade da Beira Interior, Portugal
2016 Ionic liquids in chromatography: application in plasmid DNA purification
(Thesis) Main arguer
Kamila Joanna Daniluk (Other)
Universidade da Beira Interior, Portugal

Committee member

Activity description
Role
Institution / Organization
2020/12 - Current Integrated member of CDRSP
Member
Instituto Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2015/01/01 - 2018/06/01 Pharmaceutical Biotechnology Pharmaceutical Biotechnology Universidade da Beira Interior, Portugal
2014 - 2017 Microbiology Microbiology (Licenciatura) Universidade da Beira Interior, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2021 - Current Frontiers in Bioengineering and Biotechnology Frontiers
2020/01/01 - Current Journal of Clinical Medicine (2077-0383) MDPI AG
2019/10/01 - Current Materials (1996-1944) MDPI
2019/10/01 - Current Molecules (1420-3049) MDPI AG
2019/10/01 - Current Bioengineering (2306-5354) MDPI AG
2019/10/01 - Current International Journal of Molecular Sciences (1422-0067) MDPI AG
2019/09 - Current Pharmaceutics (1999-4923) MDPI AG
2020 - 2022 Pharmaceutics MDPI

Mentoring / Tutoring

Topic Student name
2014/01/01 - 2018/08/31 Evaluation of the purification conditions of plasmid DNA regarding its biological activity for cancer therapy Gilson Martins de Azevedo Junior
2015/09/01 - 2016/09/01 Construction of an expression vector of Transtirretin Vera Mónica de Almeida Xistra
2015/07/01 - 2015/07/31 Biotechnological process for the sc p53 encoding plasmid recovery and delivery Nour Mziou
2014/07/01 - 2014/07/31 Biotechnological process for the sc p53 encoding plasmid recovery and delivery Gi Soo Kang
Distinctions

Award

2021 Best presentation award
International Manufacturing Forum Series , Portugal
2013 Individual doctoral reserach grant
Fundação para a Ciência e a Tecnologia, Portugal

Other distinction

2021 Topical Advisory Panel Member of Pharmaceutics
MDPI AG, Switzerland