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I hold a Biology degree from the University of Coimbra and an MSc and PhD in Biotechnology from Instituto Superior Técnico, UL. During my postdoc at ITQB NOVA, I studied the archaeon Pyrococcus furiosus, uncovering its accumulation of organic solutes in response to extreme heat and challenging long-standing concepts of microbial physiology. This led to their application as stabilizers (5 papers, 120+ citations, 1 patent). As a senior researcher in Adriano Henriques' lab, I investigated Bacillus subtilis spore coat enzymes, particularly a laccase now widely recognized (2 papers, 500+ citations). In 2004, I founded the Microbial and Enzyme Technology Lab at ITQB NOVA, focusing on bacterial enzymes for lignocellulose decomposition, particularly lignin. Unlike fungi, bacterial systems remain underexplored but offer advantages in gene cloning, protein production, and enzyme engineering. I became an Assistant Professor in 2014, the same year I was awarded an FCT Investigator Consolidator Grant (scored 9/9). I was promoted to Associate Professor in 2022 and defended my Agregação in Molecular Biosciences in 2024. My research in Molecular Biotechnology aims to develop novel enzymes for biomass valorization—an EU priority for sustainable chemicals, energy, and fuels. I have published 100+ papers, with six journal cover highlights, three book chapters, and 80+ invited talks. I have led six national projects, contributed to ten more, and secured EC funding for SOPHIED, BIORENEW, and SMARTBOX. I coordinated the B-LigZymes H2020 project (2019-2024) and now the MSCA Doctoral Network COMENZE (2025-2029). I have supervised 9 postdocs, 15 PhDs, 20 MScs, 25+ graduate students, and 50+ undergraduates. Since 2013, I have served on the Scientific Committee of the PhD Program in Sustainable Chemistry and contributed to ITQB NOVA’s Pedagogical Council and Infrastructures Committee. Beyond ITQB NOVA, I was part of the Bio-Based Industries Joint Undertaking (2014-2020), and I currently serve on the Executive Board of the Portuguese Society of Biotechnology, of the European Federation of Biotechnology, where I am also co-chair of the Biocatalysis Division.
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Identification

Personal identification

Full name
Lígia Maria Oliveira Martins

Citation names

  • Martins, Lígia
  • Martins, Lígia O.

Author identifiers

Ciência ID
731B-B843-C1DF
ORCID iD
0000-0003-0082-9591
Google Scholar ID
https://scholar.google.pt/citations?user=OYWueH0AAAAJ&hl=en
Researcher Id
C-2513-2009
Scopus Author Id
7102109314

Email addresses

  • lmartins@itqb.unl.pt (Professional)

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology - Biocatalysis
  • Engineering and Technology - Environmental Biotechnology - Environmental Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Intermediate (B1) Intermediate (B1) Beginner (A1) Intermediate (B1)
Portuguese (Mother tongue)
Education
Degree Classification
2024/01/18
Concluded
 Biociências Moleculares (Título de Agregado)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1994
Concluded
 PhD in Biotechnology (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Via Biossintética dos Nucleótidos de Açúcar Envolvidos na Produção do Exopolissacárido Gelano por Sphingomonas paucimobilis" (THESIS/DISSERTATION)
 Distinção e louvor
1990
Concluded
 MSc in Biological Engineering/Biotechnology (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Fisiologia da Biossíntese de Alginato por Pseudomonas aeruginosa: Enzimas Biossintéticas em Variantes Mucosas e Não Mucosas e Efeitos dos Iões Mn2+, Mg2+ e Ca2+" (THESIS/DISSERTATION)
 Very Good
1987
Concluded
 Degree (“Licenciatura”) in Biology (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Efeito da Temperatura e do Cálcio na Composição em Ácidos Gordos de Bacillus stearothermophilus" (THESIS/DISSERTATION)
 16/20
Affiliation

Science

Category
Host institution 		Employer
2000/09/01 - 2003/12/31 Auxiliary Researcher (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1990 - 1991 Visiting Researcher (Research) The University of Edinburgh Institute of Cell Biology, United Kingdom

Teaching in Higher Education

Category
Host institution 		Employer
2022/01/26 - Current Associate Professor (University Teacher) Universidade Nova de Lisboa, Portugal
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014/09/01 - Current Assistant Professor (University Teacher) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017/06/01 - 2017/06/30 Visiting Professor (University Teacher) Université Claude Bernard Lyon 1, France
2004/01/01 - 2014/08/31 Invited Assistant Professor (University Teacher) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1998/03/01 - 2005/12/31 Assistant Professor (University Teacher) Universidade Lusófona de Humanidades e Tecnologias, Portugal

Others

Category
Host institution 		Employer
2004/10/01 - Current Principal Investigator of the Microbial and Enzyme Technology Laboratory Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014/07/01 - 2017/06/30 Special Visiting Researcher Fellow Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Brazil
1994/05/01 - 2000/03/31 Post-doc Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Projects

Grant

Designation Funders
2025/03 - 2029/02 COMENZE: Computational and Enzyme Engineering for New Polymers
GA 101169327
Principal investigator
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Ongoing
2024/12 - 2028/12 McGEA: Metalloenzymes and Cells for Green Environmental Alternatives
GA 101183014
Other
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Ongoing
2024/06 - 2026/06 COxEnzymes-Ags: New Carbohydrate Oxidising Enzymes prepared by Directed Evolution and their application in the synthesis of Aminoglycoside derivatives with antibiotic potential and rare sugars.
101152159
Co-Principal Investigator (Co-PI)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Ongoing
2023 - 2026 Biocat4Suschem: Broadening the spectrum of biocatalysts, bioprocesses and bio-products in industrial biotechnology
FCT 2022.02027.PTDC
Principal investigator
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2022 - 2026 COZYME - Pan-European Network on Computational Redesign of Enzymes
CA21162
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Horizon 2020
Ongoing
2019 - 2024 B-LigZymes: Bacterial Enzymes and Bioprocesses for Lignin Valorisation
H2020-MSCA-RISE 2018. Project ID 824017
Principal investigator
EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Ongoing
2022 - 2023 Unravel Structure-Function Relationships for Enhanced Biocatalysts
EXPL/BIA-BQM/0473/2021
Integration into Research Grant Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019 - 2023 SMARTBOX: Selective Modifications of Aromatics through Biocatalytic Oxidations
H2020-BBI-JTO-2018 Project ID 837890
Integration into Research Grant Fellow
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
 European Union´s H2020 Research and Innovation Programme
Ongoing
2018 - 2021 FITzymes: Iterative Laboratory-Computational Evolution of Bacterial Ligninolytic Enzymes
PTDC/BII-BBF/29564/2017
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2021 ORBioelectronics: Oxidoreductase-based electrochemical biosensors and bioreactors: rational design guided by biophysical characterization
PTDC/BIA-BFS/31026/2017
Integration into Research Grant Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 Biocatalysis for tackling Lignin Recalcitrance
PTDC/BBB-EBB/0122/2014
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2014 - 2017 Biocatalysis for Tackling Lignin Recalcitrance and Valorisation
Ciência sem Fronteiras: Bolsa de Pesquisante Visitante Especial
Invited Scientist Fellow
Programa Ciência sem Fronteiras
Concluded
2014 - 2015 Discovery and characterization of ligninolytic bacterial enzymes
EXP/BBB-BIO/1932/2013
Integration into Research Grant Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2010/03/01 - 2013 Valorization of the Mediterranean energy crops giant reed and cardoon by integrated bio-chemical conversion to dissolving grade pulps, fuel ethanol, xylitol and lignin-based products - a complex LCF biorefinery concept.
PTDC/AGR-CFL/103840/2008
Integration into Research Grant Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2007/09/01 - 2011 Enzymatic Degradation and Synthesis of Azo and Antraquinonic Dyes
FCT/3599-PPCDT/72108/PT
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2006 - 2010 White Biotechnology for Added Value Products from Renewable Plant Polymers: Design of Tailor-Made Biocatalysts and New Industrial Processes (BIORENEW)
FP6-2004-NMP-NI-4/026456
Integration into Research Grant Fellow
European Commission Sixth Framework Programme
Concluded
2005 - 2008 NanoEngineering of Bacterial Laccases
POCTI/BIO/57083/2004
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2004 - 2008 Novel Sustainable Processes for the European Colour Industries (SOPHIED)
FP6-NMP2-CT-2004-505899
Integration into Research Grant Fellow
European Commission Sixth Framework Programme
Concluded
2006 - 2007 Expression, Purification, Biochemical and Structural Characterization of a Multicopper Oxidase from Myxococcus xanthus
E-67/2006
Principal investigator
Acção Integrada Luso-Espanhola, CRUP
Concluded
Outputs

Publications

Book chapter
  1. Martins, Lígia O.; Melo, Eduardo Pinho; Sanchez-Amat, Antonio; Robalo, Maria Paula. "Bacterial Laccases: Some Recent Advances and Applications". In Laccases in Bioremediation and Waste Valorisation, 27-55. Springer International Publishing, 2020.
    10.1007/978-3-030-47906-0_2
  2. Mendes, Sónia; Robalo, Maria Paula; Martins, Lígia O.. "Bacterial enzymes and multi-enzymatic systems for cleaning-up dyes from the environment". Springer International Publishing, 2015.
    10.1007/978-3-319-10942-8_2
  3. Henriques, A.O.; Costa, T.V; Martins, Lígia O.; Zilhão, R.. "Functional architecture and assembly of the spore coat". In "Bacterial Spore Formers: Probiotics and Emerging applications. London, United Kingdom: Horizon Scientific Press, 2004.
    Published
Conference paper
  1. Brissos, V.; Tavares, D.; Duarte, Z; Lígia O. Martins. "Engineering bacterial ligninolytic enzymes for tackling lignin recalcitrance". Paper presented in 15th European Workshop on Lignocellulosics and Pulp, Aveiro, 2018.
  2. "Improving a bacterial pyranose 2-oxidase using a combination of rational design and directed evolution for biosensor applications". Paper presented in 18th European Congress of Biotechnology, Geneva, 2018.
    Published
  3. "Towards a sustainable production of biologically active building blocks from dye-rich wastewaters". Paper presented in 18th European Congress of Biotechnology, Geneva, 2018.
    Published
  4. Sousa, Ana Catarina; Santos, Iolanda; Martins, Lígia O.; Robalo, Maria Paula. "Biocatalytic synthesis of 1,2-naphtoquinones derivatives mediated by CotA-laccase". 2017.
  5. Sousa, AC.; Martins, Lígia O.; ROBALO, MP. "Laccases: biocatalysts towards new heterocyclic cores". 2016.
  6. Sousa, A.C.; Martins, Lígia O.; Robalo, M.P.. "CotA-laccase as biocatalyst in the “green” synthesis of phenazine and acridine cores". 2016.
  7. Fernandes, AT.; Melo, EP.; Martins, Lígia O.. "Stability studies in the CotA-laccase from Bacillus subtilis and in the metallo-oxidase McoA from Aquifex aeolicus". Paper presented in Oxidative Enzymes as Sustainable Industrial Biocatalysts Workshop, 2010.
  8. Durão, P.; Martins, Lígia O.. "Towards the industrial application of biocatalysts: Elucidation of structural/ functional features of CotA-laccase". Paper presented in 6th International Conference Textile and Polymer Biotechnology, Ghent, 2009.
  9. Durão, P.; Chen, Z.; Pereira, M.M.; Todorovic, S.; Bento, I.; Lindley, P.F.; Martins, Lígia O.. "Evidences for the role of glutamate 498 as a proton donor in the reduction of dioxygen by CotA-laccase". Paper presented in 34th Congress of the Federation of European Biochemical Societies, Prague, 2009.
  10. Chen, Z.; Durão, P.; Fernandes, AT.; Hildebrandt, P.; Murgida, D.H.; Todorovic, S.; Pereira, M.M.; Melo, E.P.; Martins, Lígia O.. "Copper incorporation into recombinant CotA-laccase from Bacillus subtilis: Characterization of fully copper loaded enzymes". Paper presented in 13th International Biotechnology Symposium and Exhibition. “Biotechnology for the Sustainability of Human Society”, Dalían, 2008.
  11. Pereira, L.; Viegas, C.; Coelho, A.; Robalo, M.P.; Martins, Lígia O.. "Mechanisms of enzymatic degradation of azo and anthraquinone dyes by bacterial CotA-laccase". Paper presented in 13th European Congress on Biotechnology, Barcelona, 2007.
  12. Fernandes, AT.; Martins, Lígia O.; Melo, EP.. "Insight into stability of multicopper oxidases". Paper presented in 13th European Congress on Biotechnology, Barcelona, 2007.
  13. Durão, P.; Chen, Z.; Fernandes, AT.; Pereira, MM.; Melo, EP.; Martins, Lígia O.. "Production and characterization of a recombinant Cu-full loaded bacterial laccase". Paper presented in 13th European Congress on Biotechnology, Barcelona, 2007.
  14. Melo, EP.; Fernandes, AT.; Durão, P.; Martins, Lígia O.. "Insight into stability of CotA-laccase from the spore coat of Bacillus subtilis". Paper presented in 7th International Conference on Protein Stabilization, Exeter, 2007.
  15. Henriques, AO.; Martins, Lígia O.; Soares, CM.; Costa, T.; Pereira, MM.; Teixeira, M.. "Studies on CotA, a laccase associated with the surface of Bacillus subtilis endospores". Paper presented in 10th Int Conf on Bioinorganic Chemistry, Florence, 2001.
  16. Sá-Correia, I.; Martins, Lígia O.; Leitão, JH.; Fialho, A.M.; Monteiro, GA.; Lemos, PC.; Rodrigues, PL.. "Manipulation of exopolysaccaride synthesis in Pseudomonas aeruginosa and Pseudomonas elodea". Santiago de Compostela, 1992.
Journal article
  1. Hermann, Eniko; Rodrigues, Carolina F.; Martins, Lígia O.; Peterbauer, Clemens; Oostenbrink, Chris. "Engineering A-type Dye-decolorizing Peroxidases by Modification of a Conserved Glutamate Residue". ChemBioChem (2024): http://dx.doi.org/10.1002/cbic.202300872.
    10.1002/cbic.202300872
  2. Anja Kostelac; André Taborda; Lígia O. Martins; Dietmar Haltrich; Marina Lotti. "Evolution and separation of actinobacterial pyranose and C -glycoside-3-oxidases". Applied and Environmental Microbiology (2024): https://doi.org/10.1128/aem.01676-23.
    10.1128/aem.01676-23
  3. Brissos, Vânia; Borges, Patrícia T.; Sancho, Ferran; Lucas, Maria Fátima; Frazão, Carlos; Conzuelo, Felipe; Martins, Lígia O.. "Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases". JBIC Journal of Biological Inorganic Chemistry (2024): http://dx.doi.org/10.1007/s00775-023-02040-y.
    10.1007/s00775-023-02040-y
  4. Paulo Durão; Peter Kis; Ivo M. Chelo; M. Rita Ventura; Lígia O. Martins. "Environmentally Friendly Degradation and Detoxification of Rifampicin by a Bacterial Laccase and Hydrogen Peroxide". ChemBioChem (2024): https://doi.org/10.1002/cbic.202300627.
    10.1002/cbic.202300627
  5. Kelly Frade; Célia M. Silveira; Bruno A. Salgueiro; Sónia Mendes; Lígia O. Martins; Carlos Frazão; Smilja Todorovic; Elin Moe. "Biochemical, Biophysical, and Structural Analysis of an Unusual DyP from the Extremophile Deinococcus radiodurans". Molecules (2024): https://doi.org/10.3390/molecules29020358.
    10.3390/molecules29020358
  6. Barbosa, Catarina; Rodrigues, Carolina F.; Loncar, Nikola; Martins, Lígia O.; Todorovic, Smilja; Silveira, Célia M.. "Spectroelectrochemistry for determination of the redox potential in heme enzymes: Dye-decolorizing peroxidases". BBA Advances 5 (2024): 100112. http://dx.doi.org/10.1016/j.bbadva.2023.100112.
    10.1016/j.bbadva.2023.100112
  7. N. Delprat; L.O. Martins; L.J. Blum; C.M.G. Aymard; B. Leca-Bouvier; G. Octobre; B. Doumèche. "User-friendly one-step disposable signal-on bioassay for glyphosate detection in water samples". Biosensors and Bioelectronics (2023): https://doi.org/10.1016/j.bios.2023.115689.
    10.1016/j.bios.2023.115689
  8. Lígia O. Martins. "The quest for new robust bacterial monoamine oxidases". The FEBS Journal (2023): https://doi.org/10.1111/febs.17002.
    10.1111/febs.17002
  9. Taborda, André; Frazão, Tomás; Rodrigues, Miguel V.; Fernández-Luengo, Xavier; Sancho, Ferran; Lucas, Maria Fátima; Frazão, Carlos; et al. "Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-42000-3.
    10.1038/s41467-023-42000-3
  10. Giulia Moro; Vânia Brissos; Chiara Zanardi; Lígia O. Martins; Felipe Conzuelo. "Electrochemical investigations of the multicopper oxidase from Aquifex aeolicus under direct electron transfer with carbon electrodes". Electrochimica Acta (2023): https://doi.org/10.1016/j.electacta.2023.143199.
    10.1016/j.electacta.2023.143199
  11. Maric, Ivana; Guo, Yiming; Fürst, Maximilian J. L. J.; Van Aelst, Korneel; Van den Bosch, Sander; De Simone, Mario; Martins, Lígia O.; Sels, Bert F.; Fraaije, Marco W.. "A One-Pot, Whole-Cell Biocatalysis Approach for Vanillin Production using Lignin Oil". Advanced Synthesis & Catalysis 365 22 (2023): 3987-3995. http://dx.doi.org/10.1002/adsc.202300868.
    10.1002/adsc.202300868
  12. Silva, Diogo; Rodrigues, Carolina F.; Lorena, Constança; Borges, Patrícia T.; Martins, Lígia O.. "Biocatalysis for biorefineries: The case of dye-decolorizing peroxidases". Biotechnology Advances 65 (2023): 108153. http://dx.doi.org/10.1016/j.biotechadv.2023.108153.
    10.1016/j.biotechadv.2023.108153
  13. Magalí F. Scocozza; Francisco Vieyra; Fernando Battaglini; Ligia O. Martins; Daniel H. Murgida. "Electrochemical Actuation of a DyP Peroxidase: A Facile Method for Drastic Improvement of the Catalytic Performance". ACS Catalysis (2023): https://doi.org/10.1021/acscatal.3c01530.
    10.1021/acscatal.3c01530
  14. Silva, Diogo; Sousa, Ana Catarina; Robalo, M. Paula; Martins, Lígia O.. "A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase". New Biotechnology (2022): http://dx.doi.org/10.1016/j.nbt.2022.12.003.
    10.1016/j.nbt.2022.12.003
  15. Guerra, Vera; Tiago, Igor; Aires, Aitana; Coelho, Catarina; Nunes, João; Martins, Lígia O.; Veríssimo, António. "The gastrointestinal microbiome of browsing goats (Capra hircus)". PLOS ONE 17 10 (2022): e0276262. http://dx.doi.org/10.1371/journal.pone.0276262.
    10.1371/journal.pone.0276262
  16. Mario De Simone; Laura Alvigini; Lur Alonso-Cotchico; Vânia Brissos; Jonatan Caroli; Maria Fátima Lucas; Emanuele Monza; et al. "Rationally Guided Improvement of NOV1 Dioxygenase for the Conversion of Lignin-Derived Isoeugenol to Vanillin". Biochemistry (2022): https://doi.org/10.1021/acs.biochem.2c00168.
    10.1021/acs.biochem.2c00168
  17. Vânia Brissos; Patrícia T. Borges; Reyes Núñez-Franco; Maria Fátima Lucas; Carlos Frazão; Emanuele Monza; Laura Masgrau; Tiago N. Cordeiro; Lígia O. Martins. "Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase". ACS Catalysis (2022): https://doi.org/10.1021/acscatal.2c00336.
    10.1021/acscatal.2c00336
  18. Borges, P.T.; Silva, D.; Silva, T.F.D.; Brissos, V.; Cañellas, M.; Lucas, M.F.; Masgrau, L.; et al. "Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase". Computational and Structural Biotechnology Journal 20 (2022): 3899-3910. http://www.scopus.com/inward/record.url?eid=2-s2.0-85134879064&partnerID=MN8TOARS.
    10.1016/j.csbj.2022.07.032
  19. Sousa, A.C.; Santos, I.; Piedade, M.F.M.M.; Martins, L.O.; Robalo, M.P.. "Corrigendum to: Synthesis of Substituted 4-Arylamino-1,2-naphthoquinones in One-Pot Reactions Using CotA-Laccase as Biocatalyst (Advanced Synthesis & Catalysis, (2020), 362, 16, (3380-3387), 10.1002/adsc.202000082)". Advanced Synthesis and Catalysis 364 7 (2022): 1346-1346. http://www.scopus.com/inward/record.url?eid=2-s2.0-85127008670&partnerID=MN8TOARS.
    10.1002/adsc.202200255
  20. Magalí F. Scocozza; Lígia O. Martins; Daniel H. Murgida. "Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases". International Journal of Molecular Sciences (2021): https://doi.org/10.3390/ijms222212532.
    10.3390/ijms222212532
  21. Carolina F. Rodrigues; Patrícia T. Borges; Magali F. Scocozza; Diogo Silva; André Taborda; Vânia Brissos; Carlos Frazão; Lígia O. Martins. "Loops around the Heme Pocket Have a Critical Role in the Function and Stability of BsDyP from Bacillus subtilis". International Journal of Molecular Sciences 22 19 (2021): 10862-10862. https://doi.org/10.3390/ijms221910862.
    10.3390/ijms221910862
  22. Ana Catarina Sousa; Lígia O. Martins; M. Paula Robalo. "Laccases: Versatile Biocatalysts for the Synthesis of Heterocyclic Cores". Molecules 26 12 (2021): 3719-3719. https://doi.org/10.3390/molecules26123719.
    10.3390/molecules26123719
  23. Lange, Lene; Connor, Kevin O.; Arason, Sigurjon; Bundgård-Jørgensen, Uffe; Canalis, Antonella; Carrez, Dirk; Gallagher, Joe; et al. "Developing a Sustainable and Circular Bio-Based Economy in EU: By Partnering Across Sectors, Upscaling and Using New Knowledge Faster, and For the Benefit of Climate, Environment & Biodiversity, and People & Business". Frontiers in Bioengineering and Biotechnology 8 (2021): http://dx.doi.org/10.3389/fbioe.2020.619066.
    Published • 10.3389/fbioe.2020.619066
  24. Fernandes, A.; Pinto, B.; Bonardo, L.; Royo, B.; Robalo, M.P.; Martins, L.O.. "Wasteful Azo Dyes as a Source of Biologically Active Building Blocks". Frontiers in Bioengineering and Biotechnology 9 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85109011577&partnerID=MN8TOARS.
    10.3389/fbioe.2021.672436
  25. Patrícia T. Borges; Vânia Brissos; Guillem Hernandez; Laura Masgrau; Maria Fátima Lucas; Emanuele Monza; Carlos Frazão; Tiago N. Cordeiro; Lígia O. Martins. "Methionine-Rich Loop of Multicopper Oxidase McoA Follows Open-to-Close Transitions with a Role in Enzyme Catalysis". ACS Catalysis (2020): 7162-7176. https://doi.org/10.1021/acscatal.0c01623.
    10.1021/acscatal.0c01623
  26. Robalo, Maria Paula; Martins, Lígia O.; Sousa, Ana Catarina; Santos, Iolanda; Piedade, Maria Fátima M.M.. "Synthesis of Substituted 4-Arylamino-1,2-naphthoquinones in One-Pot Reactions Using CotA-Laccase as Biocatalyst". Advanced Synthesis & Catalysis (2020): http://dx.doi.org/10.1002/adsc.202000082.
    10.1002/adsc.202000082
  27. Barbosa, Catarina; Silveira, Célia M.; Silva, Diogo; Brissos, Vânia; Hildebrandt, Peter; Martins, Lígia O.; Todorovic, Smilja. "Immobilized dye-decolorizing peroxidase (DyP) and directed evolution variants for hydrogen peroxide biosensing". Biosensors and Bioelectronics 153 (2020): 112055.
    10.1016/j.bios.2020.112055
  28. Célia M. Silveira; Elin Moe; Marco Fraaije; Lígia O. Martins; Smilja Todorovic. "Resonance Raman view of the active site architecture in bacterial DyP-type peroxidases". RSC Advances 10 (2020): 11095.
    10.1039/D0RA00950D
  29. Alain R. Puente-Santiago; Daily Rodríguez-Padrón; Xuebo Quan; Mario J. Muñoz Batista; Lígia O. Martins; Sanny Verma; Rajender S. Varma; Jian Zhou; Rafael Luque. "Unprecedented Wiring Efficiency of Sulfonated Graphitic Carbon Nitride Materials: Toward High-Performance Amperometric Recombinant CotA Laccase Biosensors". ACS Sustainable Chemistry & Engineering (2019):
    10.1021/acssuschemeng.8b05107
  30. Sousa, A.C.; Baptista, S.R.; Martins, L.O.; Robalo, M.P.. "Synthesis of Azobenzene Dyes Mediated by CotA Laccase". Chemistry - An Asian Journal 14 1 (2019): 187-193.
    10.1002/asia.201801450
  31. Alba-Molina, David; Rodríguez-Padrón, Daily; Puente-Santiago, Alain R.; Giner-Casares, Juan J.; Martín-Romero, María T.; Camacho, Luis; Martins, Lígia O.; et al. "Mimicking the bioelectrocatalytic function of recombinant CotA laccase through electrostatically self-assembled bioconjugates". Nanoscale 11 4 (2019): 1549-1554.
    10.1039/c8nr06001k
  32. Sousa, A.C.; Conceição Oliveira, M.; Martins, L.O.; Robalo, M.P.. "A Sustainable Synthesis of Asymmetric Phenazines and Phenoxazinones Mediated by CotA-Laccase". Advanced Synthesis and Catalysis 360 3 (2018): 575-583.
    10.1002/adsc.201701228
  33. Blánquez, A.; Rodríguez, J.; Brissos, V.; Mendes, S.; Martins, L.O.; Ball, A.S.; Arias, M.E.; Hernández, M.. "Decolorization and detoxification of textile dyes using a versatile Streptomyces laccase-natural mediator system". Saudi Journal of Biological Sciences 26 (2018): 913-920.
    10.1016/j.sjbs.2018.05.020
  34. Brissos, V.; Tavares, D.; Sousa, A.C.; Robalo, M.P.; Martins, L.O.. "Engineering a Bacterial DyP-Type Peroxidase for Enhanced Oxidation of Lignin-Related Phenolics at Alkaline pH". ACS Catalysis 7 5 (2017): 3454-3465.
    10.1021/acscatal.6b03331
  35. Gonçalves, I.; Nunes, C.; Mendes, S.; Martins, L.O.; Ferreira, P.; Coimbra, M.A.. "CotA laccase-ABTS/hydrogen peroxide system: An efficient approach to produce active and decolorized chitosan-genipin films". Carbohydrate Polymers 175 (2017): 628-635.
    10.1016/j.carbpol.2017.08.029
  36. Pistone, L.; Ottolina, G.; De, S.; Romero, A.A.; Martins, L.O.; Luque, R.. "Encapsulated Laccases for the Room-Temperature Oxidation of Aromatics: Towards Synthetic Low-Molecular-Weight Lignins". ChemSusChem 9 7 (2016): 756-762.
    10.1002/cssc.201501427
  37. Sousa, Ana Catarina; Piedade, M. Fatima M. M.; Martins, Ligia O.; Paula Robalo, M.. "Eco-friendly synthesis of indo dyes mediated by a bacterial laccase". Green Chemistry 18 22 (2016): 6063-6070.
    10.1039/c6gc02050j
  38. Mendes, S.; Banha, C.; Madeira, J.; Santos, D.; Miranda, V.; Manzanera, M.; Ventura, M.R.; van Berkel, W.J.H.; Martins, L.O.. "Characterization of a bacterial pyranose 2-oxidase from Arthrobacter siccitolerans". Journal of Molecular Catalysis B: Enzymatic 133 (2016): S34-S43.
    10.1016/j.molcatb.2016.11.005
  39. Brissos, V.; Ferreira, M.; Grass, G.; Martins, L.O.. "Turning a Hyperthermostable Metallo-Oxidase into a Laccase by Directed Evolution". ACS Catalysis 5 8 (2015): 4932-4941.
    10.1021/acscatal.5b00771
  40. Martins, Ligia O.; Durao, Paulo; Brissos, Vania; Lindley, Peter F.. "Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology". Cellular and Molecular Life Sciences 72 5 (2015): 911-922.
    10.1007/s00018-014-1822-x
  41. Sousa, A.C.; Piedade, M.F.M.M.; Martins, L.O.; Robalo, M.P.. "An enzymatic route to a benzocarbazole framework using bacterial CotA laccase". Green Chemistry 17 3 (2015): 1429-1433.
    10.1039/c4gc02511c
  42. Mendes, S.; Brissos, V.; Gabriel, A.; Catarino, T.; Turner, D.L.; Todorovic, S.; Martins, L.O.. "An integrated view of redox and catalytic properties of B-type PpDyP from Pseudomonas putida MET94 and its distal variants". Archives of Biochemistry and Biophysics 574 (2015): 99-107.
    10.1016/j.abb.2015.03.009
  43. Todorovic, S.; Hildebrandt, P.; Martins, L.O.. "Surface enhanced resonance Raman detection of a catalytic intermediate of DyP-type peroxidase". Physical Chemistry Chemical Physics 17 18 (2015): 11954-11957.
    10.1039/c5cp01283j
  44. Mendes, S.; Catarino, T.; Silveira, C.; Todorovic, S.; Martins, L.O.. "The catalytic mechanism of A-type dye-decolourising peroxidase BsDyP: neither aspartate nor arginine is individually essential for peroxidase activity". Catalysis Science and Technology 5 12 (2015): 5196-5207.
    10.1039/c5cy00478k
  45. Santos, Ana; Mendes, Sonia; Brissos, Vania; Martins, Ligia O.. "New dye-decolorizing peroxidases from Bacillus subtilis and Pseudomonas putida MET94: towards biotechnological applications". Applied Microbiology and Biotechnology 98 5 (2014): 2053-2065.
    10.1007/s00253-013-5041-4
  46. Lang, W.; Sirisansaneeyakul, S.; Martins, L.O.; Ngiwsara, L.; Sakairi, N.; Pathom-aree, W.; Okuyama, M.; Mori, H.; Kimura, A.. "Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1 strain from the Mariana Trench". Journal of Environmental Management 132 (2014): 155-164.
    10.1016/j.jenvman.2013.11.002
  47. Brissos, V.; Gonçalves, N.; Melo, E.P.; Martins, L.O.. "Improving kinetic or thermodynamic stability of an azoreductase by directed evolution". PLoS ONE 9 1 (2014):
    10.1371/journal.pone.0087209
  48. Sousa, A.C.; Oliveira, M.C.; Martins, L.O.; Robalo, M.P.. "Towards the rational biosynthesis of substituted phenazines and phenoxazinones by laccases". Green Chemistry 16 9 (2014): 4127-4136.
    10.1039/c4gc00901k
  49. Lang, W.; Sirisansaneeyakul, S.; Ngiwsara, L.; Mendes, S.; Martins, L.O.; Okuyama, M.; Kimura, A.. "Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR1911: Toward dye decolorization using a packed-bed metal affinity reactor". Bioresource Technology 150 (2013): 298-306.
    10.1016/j.biortech.2013.09.124
  50. Sezer, M.; Santos, A.; Kielb, P.; Pinto, T.; Martins, L.O.; Todorovic, S.. "Distinct structural and redox properties of the heme active site in bacterial dye decolorizing peroxidase-type peroxidases from two subfamilies: Resonance raman and electrochemical study". Biochemistry 52 18 (2013): 3074-3084.
    10.1021/bi301630a
  51. Goncalves, Ana Maria D.; Mendes, Sonia; de Sanctis, Daniele; Martins, Ligia O.; Bento, Isabel. "The crystal structure of Pseudomonas putida azoreductase - the active site revisited". Febs Journal 280 24 (2013): 6643-6657.
    10.1111/febs.12568
  52. Sousa, A.C.; Martins, L.O.; Robalo, M.P.. "Laccase-catalysed homocoupling of primary aromatic amines towards the biosynthesis of dyes". Advanced Synthesis and Catalysis 355 14-15 (2013): 2908-2917.
    10.1002/adsc.201300501
  53. Rosado, T.; Bernardo, P.; Koci, K.; Coelho, A.V.; Robalo, M.P.; Martins, L.O.. "Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases". Bioresource Technology 124 (2012): 371-378.
    10.1016/j.biortech.2012.08.023
  54. Fernandes, A.T.; Lopes, C.; Martins, L.O.; Melo, E.P.. "Unfolding pathway of CotA-laccase and the role of copper on the prevention of refolding through aggregation of the unfolded state". Biochemical and Biophysical Research Communications 422 3 (2012): 442-446.
    10.1016/j.bbrc.2012.05.011
  55. Silva, Catarina S.; Durao, Paulo; Fillat, Amanda; Lindley, Peter F.; Martins, Ligia O.; Bento, Isabel. "Crystal structure of the multicopper oxidase from the pathogenic bacterium Campylobacter jejuni CGUG11284: characterization of a metallo-oxidase". Metallomics 4 1 (2012): 37-47.
    10.1039/c1mt00156f
  56. Sezer, Murat; Genebra, Tania; Mendes, Sonia; Martins, Ligia O.; Todorovic, Smilja. "A DyP-type peroxidase at a bio-compatible interface: structural and mechanistic insights". Soft Matter 8 40 (2012): 10314-10321.
    10.1039/c2sm26310f
  57. Silva, Catarina S.; Damas, Joao M.; Chen, Zhenjia; Brissos, Vania; Martins, Ligia O.; Soares, Claudio M.; Lindley, Peter F.; Bento, Isabel. "The role of Asp116 in the reductive cleavage of dioxygen to water in CotA laccase: assistance during the proton-transfer mechanism". Acta Crystallographica Section D-Biological Crystallography 68 (2012): 186-193.
    10.1107/S0907444911054503
  58. Brissos, Vania; Chen, Zhenjia; Martins, Ligia O.. "The kinetic role of carboxylate residues in the proximity of the trinuclear centre in the O-2 reactivity of CotA-laccase". Dalton Transactions 41 20 (2012): 6247-6255.
    10.1039/c2dt12067d
  59. Correia, B.; Chen, Z.; Mendes, S.; Martins, L.O.; Bento, I.. "Crystallization and preliminary X-ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 1 (2011): 121-123.
    10.1107/S1744309110048220
  60. Mendes, S.; Pereira, L.; Batista, C.; Martins, L.O.. "Molecular determinants of azo reduction activity in the strain Pseudomonas putida MET94". Applied Microbiology and Biotechnology 92 2 (2011): 393-405.
    10.1007/s00253-011-3366-4
  61. Mendes, S.; Farinha, A.; Ramos, C.G.; Leitão, J.H.; Viegas, C.A.; Martins, L.O.. "Synergistic action of azoreductase and laccase leads to maximal decolourization and detoxification of model dye-containing wastewaters". Bioresource Technology 102 21 (2011): 9852-9859.
    10.1016/j.biortech.2011.07.108
  62. Fernandes, A.T.; Pereira, M.M.; Silva, C.S.; Lindley, P.F.; Bento, I.; Melo, E.P.; Martins, L.O.. "The removal of a disulfide bridge in CotA-laccase changes the slower motion dynamics involved in copper binding but has no effect on the thermodynamic stability". Journal of Biological Inorganic Chemistry 16 4 (2011): 641-651.
    10.1007/s00775-011-0768-9
  63. Bento, I.; Silva, C.S.; Chen, Z.; Martins, L.O.; Lindley, P.F.; Soares, C.M.. "Mechanisms underlying dioxygen reduction in laccases. Structural and modelling studies focusing on proton transfer". BMC Structural Biology 10 (2010):
    10.1186/1472-6807-10-28
  64. Fernandes, A.T.; Damas, J.M.; Todorovic, S.; Huber, R.; Baratto, M.C.; Pogni, R.; Soares, C.M.; Martins, L.O.. "The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity". FEBS Journal 277 15 (2010): 3176-3189.
    10.1111/j.1742-4658.2010.07725.x
  65. Chen, Zhenjia; Durão, Paulo; Silva, Catarina S.; Pereira, Manuela M.; Todorovic, Smilja; Hildebrandt, Peter; Bento, Isabel; Lindley, Peter F.; Martins, Lígia O.. "The role of Glu498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis". Dalton Transactions 39 11 (2010): 2875.
    10.1039/b922734b
  66. Fernandes, A.T.; Martins, L.O.; Melo, E.P.. "The hyperthermophilic nature of the metallo-oxidase from Aquifex aeolicus". Biochimica et Biophysica Acta - Proteins and Proteomics 1794 1 (2009): 75-83.
    10.1016/j.bbapap.2008.09.006
  67. Brissos, V.; Pereira, L.; Munteanu, F.-D.; Cavaco-Paulo, A.; Martins, L.O.. "Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes". Biotechnology Journal 4 4 (2009): 558-563.
    10.1002/biot.200800248
  68. Pereira, L.; Coelho, A.V.; Viegas, C.A.; Ganachaud, C.; Iacazio, G.; Tron, T.; Robalo, M.P.; Martins, L.O.. "On the mechanism of biotransformation of the anthraquinonic dye acid blue 62 by lacases". Advanced Synthesis and Catalysis 351 11-12 (2009): 1857-1865.
    10.1002/adsc.200900271
  69. PEREIRA, L; COELHO, AV; VIEGAS, CA; DOS SANTOS, MMC; ROBALO, MP; MARTINS, LO. "Enzymatic biotransformation of the azo dye Sudan Orange G with bacterial CotA-laccase". Journal of Biotechnology 139 1 (2009): 68-77.
    10.1016/j.jbiotec.2008.09.001
  70. Chen, Z.; Durao, P.; Fernandes, A. T.; Martins, L. O.; Melo, E. P.; Pereira, M.. "Production and characterization of a recombinant Cu-full loaded bacterial laccase (vol 131, pg S113, 2007)". Journal of Biotechnology 144 4 (2009): 341-341.
    10.1016/j.jbiotec.2009.09.018
  71. DURAO, P; CHEN, ZJ; SILVA, CS; SOARES, CM; PEREIRA, MM; TODOROVIC, S; HILDEBRANDT, P; et al. "Proximal mutations at the type 1 copper site of CotA laccase: spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants". BIOCHEMICAL JOURNAL 412 (2008): 339-346.
    10.1042/BJ20080166
  72. DURAO, P; CHEN, Z; FERNANDES, AT; HILDEBRANDT, P; MURGIDA, DH; TODOROVIC, S; PEREIRA, MM; MELO, EP; MARTINS, LO. "Copper incorporation into recombinant CotA laccase from Bacillus subtilis: characterization of fully copper loaded enzymes". JBIC Journal of Biological Inorganic Chemistry 13 2 (2008): 183-193.
    10.1007/s00775-007-0312-0
  73. Sánchez-Sutil, M.C.; Gómez-Santos, N.; Moraleda-Muñoz, A.; Martins, L.O.; Pérez, J.; Muñoz-Dorado, J.. "Differential expression of the three multicopper oxidases from Myxococcus xanthus". Journal of Bacteriology 189 13 (2007): 4887-4898.
    10.1128/JB.00309-07
  74. Melo, E.P.; Fernandes, A.T.; Durão, P.; Martins, L.O.. "Insight into stability of CotA laccase from the spore coat of Bacillus subtilis". Biochemical Society Transactions 35 6 (2007): 1579-1582.
    10.1042/BST0351579
  75. Fernandes, A.T.; Soares, C.M.; Pereira, M.M.; Huber, R.; Grass, G.; Martins, L.O.. "A robust metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus". FEBS Journal 274 11 (2007): 2683-2694.
    10.1111/j.1742-4658.2007.05803.x
  76. DURAO, P; BENTO, I; FERNANDES, AT; MELO, EP; LINDLEY, PF; MARTINS, LO. "Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: structural, biochemical, enzymatic and stability studies". JBIC Journal of Biological Inorganic Chemistry 11 4 (2006): 514-526.
    10.1007/s00775-006-0102-0
  77. Lameiro, M.H.; Lopes, A.; Martins, L.O.; Alves, P.M.; Melo, E.. "Incorporation of a model protein into chitosan-bile salt microparticles". International Journal of Pharmaceutics 312 1-2 (2006): 119-130.
    10.1016/j.ijpharm.2006.01.006
  78. Lamosa, P.; Gonçalves, L.G.; Rodrigues, M.V.; Martins, L.O.; Raven, N.D.H.; Santos, H.. "Occurrence of 1-glyceryl-1-myo-inosityl phosphate in hyperthermophiles". Applied and Environmental Microbiology 72 9 (2006): 6169-6173.
    10.1128/AEM.00852-06
  79. Zilhão, R.; Isticato, R.; Martins, L.O.; Steil, L.; Völker, U.; Ricca, E.; Moran Jr., C.P.; Henriques, A.O.. "Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis". Journal of Bacteriology 187 22 (2005): 7753-7764.
    10.1128/JB.187.22.7753-7764.2005
  80. Bento, I.; Martins, L.O.; Lopes, G.G.; Carrondo, M.A.; Lindley, P.F.. "Dioxygen reduction by multi-copper oxidases; a structural perspective". Dalton Transactions 21 (2005): 3507-3513.
    10.1039/b504806k
  81. Costa, T.; Steil, L.; Martins, L.O.; Völker, U.; Henriques, A.O.. "Assembly of An Oxalate Decarboxylase Produced under sK Control into the Bacillus subtilis Spore Coat". Journal of Bacteriology 186 5 (2004): 1462-1474.
    10.1128/JB.186.5.1462-1474.2004
  82. ENGUITA, FJ; MARCAL, D; MARTINS, LO; GRENHA, R; HENRIQUES, AO; LINDLEY, PF; CARRONDO, MA. "Substrate and doxygen binding to the endospore coat laccase from Bacillus subtilis". JOURNAL OF BIOLOGICAL CHEMISTRY 279 22 (2004): 23472-23476.
    10.1074/jbc.M314000200
  83. Enguita, F.J.; Martins, L.O.; Henriques, A.O.; Carrondo, M.A.. "Crystal structure of a bacterial endospore coat component: A laccase with enhanced thermostability properties". Journal of Biological Chemistry 278 21 (2003): 19416-19425.
    10.1074/jbc.M301251200
  84. Martins, L.O.; Soares, C.M.; Pereira, M.M.; Teixeira, M.; Costa, T.; Jones, G.H.; Henriques, A.O.. "Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat". Journal of Biological Chemistry 277 21 (2002): 18849-18859.
    10.1074/jbc.M200827200
  85. Enguita, F.J.; Matias, P.M.; Martins, L.O.; Plácido, D.; Henriques, A.O.; Carrondo, M.A.. "Spore-coat laccase CotA from Bacillus subtilis: Crystallization and preliminary X-ray characterization by the MAD method". Acta Crystallographica Section D: Biological Crystallography 58 9 (2002): 1490-1493.
    10.1107/S0907444902011575
  86. Melo, E.P.; Faria, T.Q.; Martins, L.O.; Gonalves, A.M.; Cabral, J.M.S.. "Cutinase unfolding and stabilization by trehalose and mannosylglycerate". Proteins: Structure, Function and Genetics 42 4 (2001): 542-552.
    10.1002/1097-0134(20010301)42:4<542::AID-PROT120>3.0.CO;2-4
  87. Fialho, A.M.; Martins, L.O.; Donval, M.-L.; Leitão, J.H.; Ridout, M.J.; Jay, A.J.; Morris, V.J.; Sá-Correia, I.. "Structures and properties of gellan polymers produced by Sphingomonas paucimobilis ATCC 31461 from lactose compared with those produced from glucose and from cheese whey". Applied and Environmental Microbiology 65 6 (1999): 2485-2491.
  88. Martins, L.O.; Empadinhas, N.; Marugg, J.D.; Miguel, C.; Ferreira, C.; Da Costa, M.S.; Santos, H.. "Biosynthesis of mannosylglycerate in the thermophilic bacterium Rhodothermus marinus. Biochemical and genetic characterization of a mannosylglycerate synthase". Journal of Biological Chemistry 274 50 (1999): 35407-35414.
    10.1074/jbc.274.50.35407
  89. Silva, Z.; Borges, N.; Martins, L.O.; Wait, R.; Da Costa, M.S.; Santos, H.. "Combined effect of the growth temperature and salinity of the medium on the accumulation of compatible solutes by Rhodothermus marinus and Rhodothermus obamensis". Extremophiles 3 2 (1999): 163-172.
    10.1007/s007920050112
  90. Lamosa, P.; Martins, L.O.; Da Costa, M.S.; Santos, H.. "Effects of temperature, salinity, and medium composition on compatible solute accumulation by Thermococcus spp.". Applied and Environmental Microbiology 64 10 (1998): 3591-3598.
  91. Martins, L.O.; Huber, R.; Huber, H.; Stetter, K.O.; Da Costa, M.S.; Santos, H.. "Organic solutes in hyperthermophilic Archaea". Applied and Environmental Microbiology 63 3 (1997): 896-902.
  92. Martins, L.O.; Fialho, A.M.; Rodrigues, P.L.; Sá-Correia, I.. "Gellan gum production and activity of biosynthetic enzymes in Sphingomonas paucimobilis mucoid and non-mucoid variants". Biotechnology and Applied Biochemistry 24 1 (1996): 47-54.
  93. Xavier, K.B.; Martins, L.O.; Peist, R.; Kossmann, M.; Boos, W.; Santos, H.. "High-affinity maltose/trehalose transport system in the hyperthermophilic archaeon Thermococcus litoralis". Journal of Bacteriology 178 16 (1996): 4773-4777.
    10.1128/jb.178.16.4773-4777.1996
  94. Martins, L.O.; Carreto, L.S.; Da Costa, M.S.; Santos, H.. "New compatible solutes related to di-myo-inositol-phosphate in members of the order Thermotogales". Journal of Bacteriology 178 19 (1996): 5644-5651.
    10.1128/jb.178.19.5644-5651.1996
  95. Martins, L.O.; Santos, H.. "Accumulation of mannosylglycerate and Di-myo-inositol-phosphate by Pyrococcus furiosus in response to salinity and temperature". Applied and Environmental Microbiology 61 9 (1995): 3299-3303.
  96. Martins, L.O.; Sá-Correia, I.. "Temperature profiles of gellan gum synthesis and activities of biosynthetic enzymes". Biotechnology and Applied Biochemistry 20 3 (1994): 385-395. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028189126&partnerID=MN8TOARS.
    10.1111/j.1470-8744.1994.tb00324.x
  97. Martins, L.O.; Sá-Correia, I.. "Alginate biosynthesis in mucoid recombinants of Pseudomonas aeruginosa overproducing GDP-mannose dehydrogenase". Enzyme and Microbial Technology 13 5 (1991): 385-389.
    10.1016/0141-0229(91)90199-K
  98. Alves, M.J.; Martins, L.O.; Sá-Correia, I.. "Temperature profiles and alginate synthesis in mucoid and non-mucoid variants of Pseudomonas aeruginosa". Letters in Applied Microbiology 12 6 (1991): 244-248.
    10.1111/j.1472-765X.1991.tb00550.x
  99. Martins, L.O.; Sá-Correia, I.. "Gellan gum biosynthetic enzymes in producing and nonproducing variants of Pseudomonas elodea.". Biotechnology and applied biochemistry 14 3 (1991): 357-364.
  100. Martins, L.O.; Brito, L.C.; Sá-Correia, I.. "Roles of Mn2+, Mg2+ and Ca2+ on alginate biosynthesis by Pseudomonas aeruginosa". Enzyme and Microbial Technology 12 10 (1990): 794-799.
    10.1016/0141-0229(90)90154-I
  101. Martins, Ligia O.; Brito, Luisa C.; Sa-Correia, Isabel. "Roles of Mn2+, Mg2+ and Ca2+ on alginate biosynthesis by Pseudomonas aeruginosa". Integration, the VLSI Journal 9 3 (1990): 794-799.
  102. Martins, L.O.; Jurado, A.S.; Madeira, V.M.C.. "Composition of polar lipid acyl chains of Bacillus stearothermophilus as affected by temperature and calcium". Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 1045 1 (1990): 17-20.
    10.1016/0005-2760(90)90198-7
Preprint
  1. Ligia O Martins. "Mechanistic Insights into Glycoside 3-Oxidases Involved in C-Glycoside Metabolism in Soil Microorganisms". 2023. https://doi.org/10.21203/rs.3.rs-2662172/v1.
    10.21203/rs.3.rs-2662172/v1