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Diana Machado is an Auxiliary Researcher at Unidade de Microbiologia Médica, Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL). She has a graduation in Biology (Universidade Lusófona de Humanidades e Tecnologias de Lisboa), a Master in Medical Microbiology (UNL) and a PhD in Biomedical Sciences, specialty Microbiology (IHMT/UNL). The scientific activity developed extents across the field of medical microbiology being transversal to antimicrobial resistance, epidemiology, and laboratory diagnosis of infectious diseases and aims to study the bacterial mechanisms underlying the emergence and evolution of drug resistance with epidemiological and clinical relevance aiming to use this information to develop medicines and technologies to combat bacterial infections. She is author/co-author of 2 book chapters, 60 scientific papers in peer-review national and international journals in myco(bacteriology) on drug resistance, mechanism of action of drugs, molecular epidemiology and TB diagnosis, and more than 120 communications in congress and other scientific meetings (oral and poster). She has participated in 12 R&D projects as team member (4 ongoing), 3 as co-PI (ongoing) and 12 as PI (ongoing); supervised 5 MSc students, 4 graduation students and co-supervised 4 PhD´s and 7 MSc students. Has been participating in advanced training programs in mycobacteriology, and curricular units in NOVA’s MSc and PhD programs contributing with lectures, laboratory teaching and evaluation. She is an Editorial Board Member of Journals BMC Infectious Diseases, Annals of Clinical Microbiology and Antimicrobials and Journal of Global Antimicrobial Resistance, and ad-doc reviewer for several scientific journals (publons.com/a/1176897/). The primary research activities rely on the study drug resistance in active and latent tuberculosis, understanding drug resistance mechanisms, exploring new antituberculosis drugs and targets, and new mechanisms for “killing” M. tuberculosis. Her studies also involve the evaluation of molecular systems for the detection of M. tuberculosis, screening of mutations that confer resistance to antituberculosis drugs and the direct detection of M. tuberculosis from clinical samples, evolutionary mechanisms, molecular epidemiology, comparative genomics of clinical isolates circulating in Portugal. Study of resistance mechanisms and molecular identification of nontuberculous mycobacteria. She has been involved in the laboratory diagnosis of TB and early molecular detection of M/XDR TB in Lisbon since 2006. The work done had and still has a positive impact on the decrease of TB incidence and allowed the control of M/XDRTB in Portugal allowing to establish protocols and multicenter studies in this field with e.g., Becton & Dickinson, EUCAST, ESGMYC and TB Alliance, and pharmaceuticals like Janssen, Otsuka and Tecnimede. Has participated in the validation of the SIRE KIT Nitratase for M. tuberculosis DST with the Brazilian TB lab-network. She is also involved in the development of TB nanodiagnostics in collaboration with the Nanotheranostics Group FCT/UNL. In the between, she is also studying the mechanisms of antimicrobial resistance and virulence of the Gram-negative bacterial species Acinetobacter baumannii and Escherichia coli. The most recent projects include the study of the contribution of oxidative stress to persister formation and survival using Helicobacter pylori and the role efflux inhibitors on viral infections, namely TB/HIV co-infection, focusing on host-pathogen interaction and inhibition of cell energy.
Identification

Personal identification

Full name
Diana Machado

Citation names

  • Diana Machado

Author identifiers

Ciência ID
731A-290A-31FC
ORCID iD
0000-0003-2375-2726
Google Scholar ID
https://scholar.google.pt/citations?user=zQ0nlmoAAAAJ&hl=en
Researcher Id
L-7027-2013
Scopus Author Id
35573903100

Email addresses

  • diana@ihmt.unl.pt (Professional)

Telephones

Telephone
  • 213652600 Ext.: 409 (Professional)

Addresses

  • Instituto de Higiene e Medicina Tropical. Rua da Junqueira, 100, 1349-008, Lisboa, Lisboa, Portugal (Professional)

Websites

  • http://ghtm.ihmt.unl.pt/profiles/diana-machado-2/ (Professional)
  • https://www.researchgate.net/profile/Diana_Machado (Professional)
  • https://research.unl.pt/en/persons/diana-isabel-oliveira-machado(0135e1e3-a3fe-41fe-b17a-96514eb09537).html (Professional)
  • https://publons.com/researcher/1176897/diana-machado/ (Professional)
  • https://loop.frontiersin.org/people/91577/overview (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2014
Concluded
Doutoramento em Ciencias Biomedicas, especialidade Microbiologia (Doutoramento)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
"The dynamics of drug resistance in Mycobacterium tuberculosis: exploring the biological basis of multi- and extensively drug resistant tuberculosis (MDR/XDRTB) as a route for alternative therapeutic strategies" (THESIS/DISSERTATION)
Aproved by unanimity
2009
Concluded
Mestrado em Microbiologia Médica (Mestrado)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
"Dinâmica fisiológica e mutacional da multirresistência em Mycobacterium tuberculosis (Classification 20/20)" (THESIS/DISSERTATION)
18/20
2007
Concluded
Biology (Licenciatura)
Universidade Lusófona de Humanidades e Tecnologias, Portugal
"Aplicação de Checkerboard Hybridization para identificação de Staphylococcus (Classification 19/20)" (THESIS/DISSERTATION)
15/20
Affiliation

Science

2018/12/01 - Current Auxiliary Researcher (Research)
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal

Others

2018 - Current Stimulus of Scientific Employment, Ref. CEECIND/02562/2017
Employer
Fundação para a Ciência e a Tecnologia, Portugal
2015/10/01 - 2018/09/30 Post-Doc - FCT Grant SFRH/BPD/100688/2014
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2015 - 2016 Researcher
Employer
Universidade Nova de Lisboa Saúde Global e Medicina Tropical, Portugal
2014/06/02 - 2015/09/30 Research grantee (BI) in the FCT funded project "Type-II NADH-menaquinone oxidoreductase (NDH-2) and the respiratory chain of M. tuberculosis: new therapeutic targets to fight tuberculosis”, Ref. PTDC
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2010/02/01 - 2014/11/19 PhD student - FCT Grant SFRH/BD/65060/2009
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2009/03/01 - 2010/01/31 Research grantee (PTDC/BIA-MIC/BIC-01/2008) in the FCT funded project "Mutational and physiological dynamics of drug resistance in Mycobacterium tuberculosis: the emergence of Multi-Drug Resistant Tub
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2009 - 2009 Researcher
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2006/10/01 - 2008/12/31 Specialized technician in molecular diagnosis
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2005/10/01 - 2006/10/31 Under graduate student
Employer
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Projects

Grant

Designation Funders
2008 - Current Mutational and physiological dynamics of drug resistance in Mycobacterium tuberculosis: the emergence of Multi-Drug Resistant Tuberculosis.
info:eu-repo/grantAgreement/FCT/3599-PPCDT/71280/PT
PTDC/BIA-MIC/71280/2006
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Universidade do Algarve Faculdade de Ciências e Tecnologia
2018 - 2024 Targeting latent tuberculosis: inhibition of efflux in Mycobacterium tuberculosis persister cells during dormancy as a new strategy for anti-TB drug discovery.
DM04-DRH/201
Principal investigator
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Fundação para a Ciência e a Tecnologia
2018 - 2021 MtbEPIs: Targeting efflux pumps in drug resistant Mycobacterium tuberculosis: an approach to prevent efflux-mediated resistance and boost therapy in active and latent tuberculosis
PTDC/BIA-MIC/30692/2017
Principal investigator
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Fundação para a Ciência e a Tecnologia
2018 - 2020 MagNanoP-TB/HIV: Aplicação de nanopartículas superparamagnéticas no diagnóstico rápido, simultâneo e não invasivo da infeção pelo VIH e da tuberculose.
PGG/012/2017
Principal investigator
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Gilead Sciences Lda
2012 - 2015 Type-II NADH-menaquinone oxidoreductase (NDH-2) and the respiratory chain of M. tuberculosis: new therapeutic targets to fight tuberculosis.
info:eu-repo/grantAgreement/FCT/3599-PPCDT/121859/PT
PTDC/BIA-MIC/121859/2010
Researcher
Universidade do Algarve Faculdade de Ciências e Tecnologia
2012 - 2015 Type-II NADH-menaquinone oxidoreductase (NDH-2) and the respiratory chain of M. tuberculosis: new therapeutic targets to fight tuberculosis.
info:eu-repo/grantAgreement/FCT/3599-PPCDT/121859/PT
Universidade do Algarve Faculdade de Ciências e Tecnologia

Other

Designation Funders
2011 - Current zoonTB - Improved NAT-based approaches to detect and discriminate animal-associated Mycobacterium tuberculosis Complex members and evaluation of the zoonotic potential of these species in Portugal
info:eu-repo/grantAgreement/FCT/3599-PPCDT/111634/PT
PTDC/CVT/111634/2009
Researcher
Universidade do Algarve Faculdade de Ciências e Tecnologia
2010 - Current Enhancing the killing of intracellular multi-drug resistant tuberculosis (MDRTB) by human macrophages: a new chemotherapeutic strategy to fight MDRTB.
info:eu-repo/grantAgreement/FCT/3599-PPCDT/102807/PT
PTDC/SAU-FCF/102807/2008
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Universidade do Algarve Faculdade de Ciências e Tecnologia
2006 - Current Diagnóstico e epidemiologia molecular de M/XDR-TB na Grande Lisboa: a detecção precoce na prevenção da emergência e disseminação de estirpes de M. tuberculosis extensivamente resistentes
SDH49, P-99934
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Fundação Calouste Gulbenkian
2006 - Current Desenvolvimento de sondas moleculares para o rastreio de Staphylococcus em amostras clínicas.
61056
Researcher
2018 - 2021 BYDRUG: Immunotargeting efflux systems for therapeutic modulation of multidrug resistant bacteria.
PTDC/BTMSAL/30550/2017
Researcher
Fundação para a Ciência e a Tecnologia
2018 - 2021 IonPharma4TB: Active pharmaceutical ionic liquids as new platform for effective treatment of tuberculosis (TB-ILs).
PTDC/QUIQOR/32406/2017.
Researcher
Fundação para a Ciência e a Tecnologia
2018 - 2021 TARGTUB - Targeting multi-resistant tuberculosis with new potent isoniazid derivatives: an integrated medicinal chemistry approach.
PTDC/MEDQUI/29036/2017
Researcher
Fundação para a Ciência e a Tecnologia
2018 - 2021 BIOSAFE - Preventing antimicrobial resistance in the community - the safe use of biocides.
02/SAICT/2017: C493201324-00089525
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Fundação para a Ciência e a Tecnologia
2017 - 2018 Determination of the minimum inhibitory concentration of pretomanid against M. tuberculosis using the BACTEC MGIT 960 instrument
TB Alliance Pretomanid_NCLN_Micro_002 Study
Principal investigator
2017 - 2018 Characterization of drug-resistant TB and HIV and associated socio-behavioral factors among migrants in Lisbon, Portugal.
UID/MULTI/04413/2013
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical

Universidade Nova de Lisboa Saúde Global e Medicina Tropical
2017 - 2018 Development of a reference method for MIC testing of M. tuberculosis as a basis for evidence based clinical breakpoints – ESGMYC and the EUCAST subcommittee for antimycobacterial AST.
Pretomanid_NCLN_Micro_002
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
European Society of Clinical Microbiology and Infectious Diseases
2017 - 2018 Development of a protocol for the determination of the minimum inhibitory concentration of pretomanid against M. tuberculosis using the BACTEC MGIT 960 instrument - TB Alliance for drug development.
Pretomanid_NCLN_Micro_002
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
TB Alliance
2015 - 2017 Evaluation of the Kit SIRE Nitratase assay - Brazilian TB lab-network and the company PlastLabor.
310174/2014-7
446796/2014-0
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Conselho Nacional de Desenvolvimento Científico e Tecnológico
2010 - 2014 Development of a protocol for semiquantitative drug susceptibility testing of M. tuberculosis using the MGIT 960 / TB eXiST – ESGMYC and BD TBeXIST - quantitative drug susceptibility testing (qDST) European validation study
TB eXIST_EU-QDST_2010
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2010 - 2013/08 Helper compounds against multidrug resistant bacteria: revealing their mechanism of action
Provided by PTCRIS: 105509
PTDC/BIA-MIC/105509/2008
Researcher
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Outputs

Publications

Book chapter
  1. da Silva, Pedro Eduardo Almeida; Machado, Diana; Ramos, Daniela; Couto, Isabel; Von Groll, Andrea; Viveiros, Miguel. "Efflux Pumps in Mycobacteria: Antimicrobial Resistance, Physiological Functions, and Role in Pathogenicity". In Efflux-Mediated Antimicrobial Resistance in Bacteria, 527-559. Springer International Publishing, 2016.
    10.1007/978-3-319-39658-3_21
  2. Viveiros, M.; Machado, D.; Couto, I.; Amaral, L.. "Improving on the LJ slope - automated liquid culture.". In Tuberculosis: laboratory diagnosis and treatment strategies, 34-45. CABI, 2013.
    10.1079/9781845938079.0034
Conference poster
  1. Diana Machado; Viveiros, Miguel; Pieroni, Marco. "Targeting Mycobacterium tuberculosis membrane energetics with lipophilic efflux inhibitors as adjuvants of tuberculosis treatment". Paper presented in III International Conference NOVAhealth Chronic Disease and Infection "Infection, Cancer and Global Health", 2020.
  2. Diana Machado; Ravichandran, Rushiil; Machado, Diana; Kroger, Carsten; Viveiros, Miguel; Martins, Marta. "Tackling resistance to carbapenem and colistin in clinical isolates of Acinetobacter baumannii.". Paper presented in 30th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2020.
  3. Diana Machado; Viveiros, Miguel. "ECOFFs for non-tuberculous mycobacteria: towards a EUCAST reference method and clinical breakpoints for antimicrobial susceptibility testing". Paper presented in 30th ECCMID, 2020.
  4. Diana Machado; Gothe, Sarah; Martins, Marta; Pacheco, Teresa; Batista, Judite; Toscano, Cristina; Viveiros, Miguel. "Colistin heteroresistance in carbapenemase-producing Acinetobacter baumannii". Paper presented in 30th ECCMID, 2020.
  5. Diana Machado; Viveiros, Miguel. "Tackling resistance to carbapenem and colistin in clinical isolates of Acinetobacter baumannii". Paper presented in 30th ECCMID, 2020.
  6. Pereira, Bruna (5D1E-C298-769E); Machado, Diana; Silva, Carla; Viveiros, Miguel. "Exploring the contribution of efflux on the resistance to antibiotics, biocides and dyes in clinical isolates of Escherichia coli.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  7. Serra, Débora; Machado, Diana; Silva, Carla; Viveiros, Miguel. "Detection and inhibition of ß-lactamase activity in clinical isolates of Escherichia coli combining ß-lactamase and efflux inhibitors using an MTT-based method: phenotypic and genotypic validation.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  8. Machado, Diana; Neves, Iolanda; Silva, Carla; Viveiros, Miguel. "Tackling bacterial infections by eliminating persister cells with efflux inhibitors.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  9. Machado, Diana; Pedrosa, Pedro; Piedade, João; Baptista, Pedro V.; Viveiros, Miguel. "Application of superparamagnetic nanoparticles for the early diagnosis of tuberculosis and HIV co-infection directly from respiratory samples.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  10. Maia, Inês; Machado, Diana; Viveiros, Miguel. "Evaluation of competition between substrates of the efflux pump system AcrAB-TolC of Escherichia coli using Red Nile.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  11. Roseiro, Isabel; Machado, Diana; Viveiros, Miguel. "The dark-side of drug resistance in Escherichia coli: efflux pumps and ß-lactamase producing strains.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  12. Ôchoa-Pires, Tiago; Froufe, Hugo; Barroso, Cristina; Machado, Diana; Rosa, Marguerita; Laranjeira, João; Seabra, Rafaela; et al. "Exploring Japanese quail immune repertoires for antibody discovery.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  13. Ribeiro, José; Machado, Diana; Viveiros, Miguel. "A bioinformatic approach to understand antibiotic resistance due to small multidrug resistant (SMR)-type efflux pumps in Acinetobacter baumannii through whole genome analysis.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  14. Gothe, Sarah; Machado, Diana; Martins, Marta; Pacheco, teresa; Batista, Judite; Toscano, Cristina; Viveiros, Miguel. "Insights on Acinetobacter baumannii heteroresistance to colistin: what is the fate?". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19), 2019.
  15. Machado, Diana; Pedrosa, Pedro; Piedade, João; Baptista, Pedro V.; Viveiros, Miguel. "Application of superparamagnetic nanoparticles for fast, simultaneous and non-invasive diagnosis of tuberculosis and HIV infection.". Paper presented in 40th Annual Congress of the European Society of Mycobacteriology, 2019.
  16. Machado, Diana; Lecorche, Emmanuel; Mougari, Faiza; Cambau, Emmanuelle; Viveiros, Miguel. "Insights on Mycobacterium leprae efflux pumps and their implications in drug resistance and virulence.". Paper presented in 40th Annual Congress of the European Society of Mycobacteriology, 2019.
  17. Machado, Diana; Azzali, Elisa; Couto, Isabel; Constantino, Gabriele; Pieroni, Marco; Viveiros, Miguel. "Adjuvant therapies against tuberculosis: discovery of a 2-aminothiazole targeting Mycobacterium tuberculosis energetics.". Paper presented in Multi-drug efflux systems: Gordon Research Conferences, 2019.
  18. Machado, Diana; Costa, Raquel; Neves, Iolanda; Antunes, Jéssica; Couto, Isabel; Viveiros, Miguel. "Contribution of efflux to the emergence of antibiotic resistance in persister cells.". Paper presented in 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2019.
  19. Perdigão, João; Silva, Carla; Diniz, Jaciara; Pereira, Catarina; Machado, Diana; Ramos, Jorge; Silva, Hugo; et al. "Mycobacterium tuberculosis genetic diversity and drug resistance across Portuguese-speaking countries and CPLP-TB: a novel framework and surveillance tool for the Lusophone community.". Paper presented in 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2019.
  20. Ramos, Jorge; Machado, Diana; Couto, Isabel; Viveiros, Miguel. "Diagnóstico laboratorial de micobactérias no Instituto de Higiene e Medicina Tropical: retrospetiva dos últimos 5 anos.". Paper presented in 4o Congresso Nacional de Medicina Tropical, 2019.
  21. Theron, Maria; Machado, Diana; Couto, Isabel; Viveiros, Miguel. "Determination of efflux activity of Hoechst 33258 in Escherichia coli using a 96-well plate fluorescence assay.". Paper presented in NOVAsaude IV Genetics Workshop, 2019.
  22. Rodrigues, Fátima; Machado, Diana; Antunes, Jéssica; Couto, Isabel; Pacheco, teresa; Batista, Judite; Toscano, Cristina; Viveiros, Miguel. "Colistin resistance in Acinetobacter baumannii: correlation between mutations in target genes and active efflux.". Paper presented in NOVAsaude IV Genetics Workshop, 2019.
  23. Ribeiro, José; Machado, Diana; Antunes, Jéssica; Rodrigues, Fátima; Couto, Isabel; Pacheco, teresa; Batista, Judite; Toscano, Cristina; Viveiros, Miguel. "Prevalence of genes encoding small multidrug resistant (SMR)-type efflux pumps in Acinetobacter baumannii resistant to carbapenems isolated in hospital environment.". Paper presented in NOVAsaude IV Genetics Workshop, 2019.
  24. Neves, Iolanda; Machado, Diana; Costa, Raquel; Antunes, Jéssica; Mendes, Ângela; Castro, Rita; Couto, Isabel; Viveiros, Miguel. "Persister cells survive antibiotic treatment mounting an active response via activation of efflux pumps.". Paper presented in NOVAsaude IV Genetics Workshop., 2019.
  25. Machado, Diana; Perdigão, João; Ramos, Jorge; Tavares, Ana M; Abecasis, Ana; Dias, Sónia; Clark, Taane G.; et al. "Molecular epidemiology of antibiotic resistance genes in multi- and extensively drug-resistant Mycobacterium tuberculosis in Lisbon and its correlation with drug resistance levels.". Paper presented in NOVAsaude IV Genetics Workshop, 2019.
  26. Machado, Diana; Azzali, Elisa; Couto, Isabel; Constantino, Gabriele; Pieroni, Marco; Viveiros, Miguel. "Adjuvant therapies against tuberculosis: discovery of a 2-aminothiazole targeting Mycobacterium tuberculosis energetics.". Paper presented in 1º Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal, 2018.
  27. Machado, Diana; Perdigão, João; Ramos, Jorge; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel. "Exploring the phenotypic and genetic basis of drug-resistant Mycobacterium tuberculosis strains in Lisbon, Portugal.". Paper presented in 1º Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal, 2018.
  28. Perdigão, João; Silva, Carla; Diniz, Jaciara; Pereira, Catarina; Machado, Diana; Ramos, Jorge; Silva, Hugo; et al. "CPLP-TB: a novel framework and surveillance tool for tracing Mycobacterium tuberculosis strains across Lusophone countries.". Paper presented in 1º Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal, 2018.
  29. Costa, Sofia S; Lopes, Elizeth; Pieroni, Marco; Machado, Diana; Constantino, Gabriele; Viveiros, Miguel; Couto, Isabel. "Mycobacterium smegmatis model for the rapid screening of new compounds with efflux inhibitory and antimycobacterial activity.". Paper presented in 1º Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal, 2018.
  30. Machado, Diana; Perdigão, João; Couto, Isabel; Silva, Pedro A.; Viveiros, Miguel. "Efflux activity differentially modulates the levels of isoniazid and rifampicin resistance among multidrug resistant and monoresistant Mycobacterium tuberculosis strains.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec17), 2017.
  31. Brandão, Ângela; Machado, Diana; Pinhata, Juliana; Oliveira, Rosangela; Ferrazoli, Lucilaine; Chimara, Erica; Viveiros, Miguel. "Reliability of a multiplex allele-specific polymerase chain reaction (MAS-PCR) for the detection of multidrug-resistant Mycobacterium tuberculosis clinical isolates from Brazil.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec17), 2017.
  32. Antunes, Jéssica; Machado, Diana; Couto, Isabel; Pacheco, teresa; Batista, Judite; Toscano, Cristina; Viveiros, Miguel. "Correlation between carbapenem resistance, ß-lactamases, porins and efflux pumps in Acinetobacter baumannii clinical isolates.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec17), 2017.
  33. Silva, Mariana; Machado, Diana; Ramos, Jorge; Couto, Isabel; Viveiros, Miguel. "The contribution of efflux to ß-lactams resistance in non-ESBL-producing Escherichia coli clinical isolates.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec17), 2017.
  34. Laranjeira, João; Rosa, Marguerita; Machado, Diana; Francisco, Rafael; Viveiros, Miguel; Vieira-Pires, Ricardo. "Fighting bacterial infections with avian IgY antibodies.". Paper presented in Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec17), 2017.
  35. Silva, Mariana; Machado, Diana; Ramos, Jorge; Couto, Isabel; Viveiros, Miguel. "The interplay between efflux and ß-lactamase activity in ß-lactams resistance in non-ESBL-producing Escherichia coli clinical isolates".". Paper presented in NOVAsaude III Genetics Workshop, 2017.
    10.5220/0007540802490252
  36. Costa, Raquel; Machado, Diana; Couto, Isabel; Viveiros, Miguel. "Contribution of efflux to the emergence of antibiotic resistance in persister cells.". Paper presented in NOVAsaúde III Genetics Workshop, 2017.
  37. Marrinhas, Carla; Santos, Marta; Miranda, Sónia; Fernandes, Laura; Rodrigues, Catarina; Machado, Diana; Miguel, Viveiros; Pomba, Constança; Marcos, Ricardo. "Cytological and molecular diagnosis of infection by Mycobacterium avium: report of a clinical case.". Paper presented in XXII Meeting of the Portuguese Society of Animal Pathology, 2017.
  38. Machado, Diana; Coelho, Tatiane; Perdigão, João; Pereira, Catarina; Couto, Isabel; Portugal, Isabel; Maschmann, Raquel; et al. "Correlação entre a presença de mutações e a actividade de efluxo em isolados clínicos de Mycobacterium tuberculosis resistente aos antibióticos.". Paper presented in 3o Congresso Nacional de Medicina Tropical, 2017.
  39. Silva, Mariana; Machado, Diana; Couto, Isabel; Viveiros, Miguel. "A contribuição do efluxo na resistência aos ß-lactâmicos em Escherichia coli.". Paper presented in 3o Congresso Nacional de Medicina Tropical, 2017.
  40. Antunes, Jéssica; Machado, Diana; Couto, Isabel; Pacheco, teresa; Batista, Judite; Toscano, Cristina; Viveiros, Miguel. "Caracterização fenotípica e relação clonal entre isolados clínicos de Acinetobacter baumannii resistentes aos carbapenemos.". Paper presented in 3o Congresso Nacional de Medicina Tropical. Lisbon, Portugal, 2017.
  41. Costa, Raquel; Machado, Diana; Couto, Isabel; Viveiros, Miguel. "Estudo do efeito de fontes de carbono e inibidores de efluxo na susceptibilidade aos antibióticos em Escherichia coli.". Paper presented in 3º Congresso Nacional de Medicina Tropical, 2017.
  42. Laranjeira, João; Machado, Diana; Francisco, Rafael; Rosa, Marguerita; Viveiros, Miguel; Vieira-Pires, Ricardo. "An avian antibody platform to fight bacterial infections.". Paper presented in 3rd Biologics and Biosimilars Congress, 2017.
  43. Marrinhas, Carla; Santos, Marta; Marcos, Ricardo; Miranda, Sónia; Fernandes, Laura; Rodrigues, Catarina; Machado, Diana; Viveiros, Miguel; Pomba, Constança. "Infecção por Mycobacterium avium num gato em Portugal.". Paper presented in 7º Encontro de Formação da Ordem dos Médicos Veterinários, 2016.
  44. Felicetti, Tommaso; Cannalire, Rolando; Manfroni, Giuseppe; Tabarrini, Oriana; Machado, Diana; Couto, Isabel; Costa, Sofia S; et al. "Natural isoflavone biochanin-A as a template for the design of new and potent 3-phenylquinolone inhibitors of the nontuberculous mycobacteria efflux pumps.". Paper presented in XXIV National Meeting in Medicinal Chemistry and 10th Young Medicinal Chemists´ Symposium, 2016.
  45. Perdigão, João; Maltez, Fernando; Machado, Diana; Silva, Hugo; Silva, Carla; Couto, Isabel; Viveiros, Miguel; Portugal, Isabel. "Extensively drug resistant tuberculosis plus linezolid resistance: the first report of linezolid resistance development in Lisbon, Portugal.". Paper presented in 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2016.
  46. Perdigão, João; Clemente, Sofia; Ramos, Jorge; Masakidi, Pedro; Machado, Diana; Silva, Carla; Couto, Isabel; et al. "First insights on the drug resistance and genetic diversity of Mycobacterium tuberculosis in Luanda, Angola.". Paper presented in 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2016.
  47. Machado, Diana; Perdigão, João; Ramos, Jorge; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel. "Exploring the phenotypic and genetic basis of drug-resistant Mycobacterium tuberculosis strains in Lisbon, Portugal.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec15)., 2015.
  48. Machado, Diana; Couto, Isabel; Viveiros, Miguel. "Drug susceptibility testing for Mycobacterium avium complex strains using the MGIT960 system and the TB-eXIST software.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec15), 2015.
  49. Machado, Diana; Serra, Carlos; Viveiros, Miguel; Couto, Isabel. "Characterization of the mechanism of action of efflux inhibitors on the mycobacterial respiratory chain.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec15), 2015.
  50. Azzali, Elisa; Pieroni, Marco; Machado, Diana; Costa, Sofia S; Couto, Isabel; Viveiros, Miguel; Constantino, Gabriele. "Rational design and synthesis of thioridazine analogues as enhancers of the antituberculosis therapy.". Paper presented in SIMCC2015 - Spanish-Italian Medicinal Chemistry Congress, 2015.
  51. Perdigão, João; Clemente, Sofia; Ramos, Jorge; Masakidi, Pedro; Machado, Diana; Silva, Carla; Couto, Isabel; et al. "Drug resistance and genetic diversity of Mycobacterium tuberculosis in Luanda, Angola: a molecular epidemiological perspective.". Paper presented in 36th Annual Congress of the European Society of Mycobacteriology, 2015.
  52. Rabna, Paulo; Ramos, Jorge; Ponce, Gema; Sanca, Lilica; Mane, Morto; Armada, Ana; Machado, Diana; et al. "Laboratory fast direct detection and characterization of multi and poly drug-resistant tuberculosis in Guinea-Bissau.". Paper presented in 3º Congresso Nacional de Medicina Tropical, 2015.
  53. Coelho, Tatiane; Machado, Diana; Machmann, Raquel; von Groll, Andrea; Rossetti, Maria L; da Silva, Pedro E. Almeida; Viveiros, Miguel. "Efluxo como mecanismo envolvido na resistência antimicrobiana em isolados clínicos de Mycobacterium tuberculosis.". Paper presented in VII meeting of the Latino-American Society of Tuberculosis and other Mycobacteriosis, 2014.
  54. Machado, Diana; Ramos, Jorge; Couto, Isabel; Cadir, Nureisha; Narciso, Inácio; Coelho, Elisabete; Viegas, Sofia; Viveiros, Miguel. "Assessment of the BD MGIT TBc identification test for the detection of Mycobacterium tuberculosis complex in a network of mycobacteriology laboratories.". Paper presented in 35th Annual Congress of the European Society of Mycobacteriology, 2014.
  55. Machado, Diana; Silva, Vânia; Couto, Isabel; Ramos, Jorge; Viveiros, Miguel. "The interplay between mutations and efflux in acquired resistance to clarithromycin in Mycobacterium avium complex.". Paper presented in 24th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2014.
  56. Machado, Diana; Perdigão, João; Ramos, Jorge; Couto, Isabel; Portugal, Isabel; Viveiros, Miguel. "Correlation between phenotypic and genetic resistance to first and second line antituberculosis drugs in a high tuberculosis endemic area.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec13), 2013.
  57. Machado, Diana; Pires, David; Perdigão, João; Ramos, Jorge; Couto, Isabel; Portugal, Isabel; ANES, ELSA; Viveiros, Miguel. "Efflux inhibitors against drug resistant Mycobacterium tuberculosis: antimicrobial agents and enhancers of macrophage killing activity.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec13), 2013.
  58. Silva, Vânia; Machado, Diana; Ramos, Jorge; Couto, Isabel; Viveiros, Miguel. "Contribution of efflux systems to clarithromycin resistance in Mycobacterium avium complex.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec13), 2013.
  59. Serra, Carlos; Fernandes, Ana; Machado, Diana; Viveiros, Miguel; Salgueiro, Carlos; Couto, Isabel. "The role of type-II NADH-menaquinone oxidoreductase (NDH-2) on Mycobacterium tuberculosis resistance to drugs.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec13), 2013.
  60. Perdigão, João; Macedo, Rita; Machado, Diana; Silva, Carla; Jordão, Luísa; Couto, Isabel; Viveiros, Miguel; Portugal, Isabel. "Polymorphism in gidB gene as a genetic marker for the Mycobacterium tuberculosis Q1 cluster and implications for the streptomycin resistance level.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec13), 2013.
  61. Perdigão, João; Silva, Hugo; Machado, Diana; Macedo, Rita; Maltez, Fernando; Silva, Carla; Jordão, Luísa; et al. "Genomic diversity of drug-resistant Mycobacterium tuberculosis isolates in Lisbon Portugal: towards tuberculosis genomic epidemiology.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec13), 2013.
  62. Perdigão, João; Macedo, Rita; Machado, Diana; Silva, Carla; Jordão, Luísa; Couto, Isabel; Viveiros, Miguel; Portugal, Isabel. "Correlation between streptomycin intermediate level resistance and gidB mutation in an endemic multidrug-resistant tuberculosis cluster.". Paper presented in 34th Annual Congress of the European Society of Mycobacteriology, 2013.
  63. Machado, Diana; Couto, Isabel; Ramos, Jorge; Viveiros, Miguel. "Contribution of efflux pumps to overall antibiotic resistance in Mycobacterium tuberculosis and the usefulness of efflux inhibitors as “helper compounds” in drug therapy". Paper presented in 23rd European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2013.
  64. Pedrosa, Pedro; Viegas, Bruno; Machado, Diana; Perdigão, João; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro V.. "Gold nanoprobes methodology for diagnosis of multi-drug resistant tuberculosis.". Paper presented in NanoPT, 2013.
  65. Gröblacher, Barbara; Armada, Ana; Machado, Diana; Viveiros, Miguel; Bucar, Franz. "Mycobacterial efflux inhibitors from Aframomum melegueta.". Paper presented in II International Conference on Antimicrobial Research (ICAR), 2012.
  66. Machado, Diana; Couto, Isabel; Ramos, Jorge; Viveiros, Miguel. "Efflux inhibitors as adjuvants in drug resistant tuberculosis therapy". Paper presented in EMBO Conference: Tuberculosis 2012: Biology, pathogenesis, intervention strategies, 2012.
  67. Perdigão, João; Silva, Diogo; Pereira, Vânia; Silva, Carla; Machado, Diana; Couto, Isabel; Viveiros, Miguel; Jordão, Luísa; Portugal, Isabel. "Dynamics and development of extensively drug-resistant tuberculosis, Portugal.". Paper presented in EMBO Conference: Tuberculosis 2012: Biology, pathogenesis, intervention strategies, 2012.
  68. Machado, Diana; Rodrigues, Liliana; Couto, Isabel; Amaral, Leonard; Viveiros, Miguel. "The emergence of clarithromycin resistance in Mycobacterium avium complex.". Paper presented in 22nd European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2012.
  69. Pinto, Antónia; Machado, Diana; Costa, Sofia S; Couto, Isabel; Amaral, Leonard; Viveiros, Miguel. "Multidrug resistance mediated by active efflux in Escherichia coli: new therapeutic strategies using antibiotics and inhibitors of active efflux.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec11), 2011.
  70. Francisco, Samuel; Machado, Diana; Viveiros, Miguel; Ramos, Jorge; Pacheco, teresa; Melo-Cristino, José; Lito, Luís M; et al. "Application of molecular methods for the direct detection of MDR-TB.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec11), 2011.
  71. Cerca, Pedro; Machado, Diana; Viveiros, Miguel; Amaral, Leonard; Couto, Isabel. "Identification of nontuberculous mycobacteria: comparison of three in-house molecular protocols.". Paper presented in 51st Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 2011.
  72. Machado, Diana; Couto, Isabel; Rodrigues, Liliana; Amaral, Leonard; Viveiros, Miguel. "Efflux activity in Mycobacterium tuberculosis as an intrinsic mechanism of resistance to isoniazid.". Paper presented in 32nd Annual Congress of the European Society of Mycobacteriology, 2011.
  73. Machado, Diana; Couto, Isabel; Amaral, Leonard; Portugal, Isabel; Baptista, Pedro V.; Viveiros, Miguel. "Selection for mutator phenotypes in Mycobacterium tuberculosis.". Paper presented in 32nd Annual Congress of the European Society of Mycobacteriology, 2011.
  74. Francisco, Samuel; Machado, Diana; Viveiros, Miguel; Ramos, Jorge; Pacheco, teresa; Melo-Cristino, José; Lito, Luís M; et al. "Application of molecular methods for the direct detection of MDR-TB.". Paper presented in 32nd Annual Congress of the European Society of Mycobacteriology, 2011.
  75. Machado, Diana; Couto, Isabel; Rodrigues, Liliana; Perdigão, João; Portugal, Isabel; Amaral, Leonard; Viveiros, Miguel. "Phenotypic adaptation to isoniazid in Mycobacterium tuberculosis: a pathway to multidrug resistant tuberculosis.". Paper presented in 21st European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2011.
  76. Rodrigues, Liliana; Machado, Diana; Couto, Isabel; Amaral, Leonard; Viveiros, Miguel. "Contribution of efflux activity to isoniazid resistance in Mycobacterium tuberculosis complex.". Paper presented in 21st European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), 2011.
  77. Viegas, Bruno; Machado, Diana; Perdigão, João; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro V.. "A PCR-Au-nanoprobes combined approach for detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis.". Paper presented in 11th Trends in Nanotechnology International Conference (TNT2010), 2010.
  78. Rodrigues, Liliana; Machado, Diana; Couto, Isabel; Amaral, Leonard; Viveiros, Miguel. "Contribution of efflux activity to isoniazid resistance in Mycobacterium tuberculosis complex.". Paper presented in COST ACTION BM0701 MEETING, 2010.
  79. Ramos, Jorge; Machado, Diana; Couto, Isabel; Amaral, Leonard; Viveiros, Miguel. "Evaluation of the BD MGIT™ TBc identification test for the rapid detection of Mycobacterium tuberculosis complex from cultures.". Paper presented in 31th Annual Congress of the European Society of Mycobacteriology, 2010.
  80. Machado, Diana; Couto, Isabel; Amaral, Leonard; Viveiros, Miguel. "Mutational adaptation in Mycobacterium tuberculosis: the emergence of multidrug resistance.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec09), 2009.
  81. Cerca, Pedro; Machado, Diana; Viveiros, Miguel; Amaral, Leonard; Couto, Isabel. "Identification of non-tuberculous mycobacteria by molecular methods: comparison of three in-house protocols.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec09), 2009.
  82. Rodrigues, Liliana; Sampaio, Daniela; Couto, Isabel; Machado, Diana; Kern, Winfried V.; Amaral, Leonard; Viveiros, Miguel. "The role played by efflux pump in macrolide resistance in Mycobacterium avium complex.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec09), 2009.
  83. Falcao, Celeste; Costa, Sofia S; Viveiros, Miguel; Machado, Diana; Martins, Marta; Melo-Cristino, José; Amaral, Leonard; Couto, Isabel. "Importance of efflux systems on the resistance to fluoroquinolones by Staphylococcus aureus.". Paper presented in Congresso de Microbiologia e Biotecnologia (MicroBiotec09), 2009.
  84. Couto, Isabel; Machado, Diana; Viveiros, Miguel; Rodrigues, Liliana; Amaral, Leonard. "Identification of nontuberculous mycobacteria in clinical samples using molecular methods: a three-year study.". Paper presented in 30th Annual Congress of the European Society of Mycobacteriology, 2009.
  85. Machado, Diana; Viveiros, Miguel; Rodrigues, Liliana; Couto, Isabel; Amaral, Leonard. "Early detection of MDRTB by molecular tools in the control of drug resistant tuberculosis in Portugal: a case of success.". Paper presented in 30th Annual Congress of the European Society of Mycobacteriology, 2009.
  86. Couto, Isabel; Viveiros, Miguel; Machado, Diana; Rodrigues, Liliana; Amaral, Leonard. "Identificação de micobactérias atípicas em amostras clínicas utilizando métodos moleculares.". Paper presented in IX Congresso Nacional de Doenças Infecciosas e Microbiologia Clínica, Sida e Parasitologia, 2008.
  87. Rodrigues, Liliana; Viveiros, Miguel; Sampaio, Daniela; Couto, Isabel; Machado, Diana; Vavra, Martina; Wagner, Dirk; Kern, Winfried V.; Amaral, Leonard. "O Papel das bombas de efluxo na resistência aos antibióticos em micobactérias do complexo M. avium.". Paper presented in IX Congresso Nacional de Doenças Infecciosas e Microbiologia Clínica, Sida e Parasitologia, 2008.
  88. Couto, Isabel; Machado, Diana; Viveiros, Miguel; Rodrigues, Liliana; Amaral, Leonard. "Molecular identification of non-tuberculosis mycobacteria from Lisbon hospitals: a two-year study.". Paper presented in MICRO’07 BIOTEC’07-XXXIII JPG, 2007.
  89. Couto, Isabel; Viveiros, Miguel; Machado, Diana; Rodrigues, Liliana; Martins, Marta; Amaral, Leonard. "Identification of non-tuberculosis mycobacteria in clinical samples using molecular methods: a two-year study.". Paper presented in 47th International Congress on Antimicrobial Agents and Chemotherapy, 2007.
  90. Machado, Diana; Costa, Marta; Lencastre, Hermínia; Couto, Isabel. "Evaluation of reverse checkerboard hybridization for staphylococcal identification and screening of antibiotic resistance genes.". Paper presented in 8th European Congress of Chemotherapy and Infection, 2006.
  91. Costa, Marta; Machado, Diana; Lencastre, Hermínia; Couto, Isabel. "Evaluation of checkerboard hybridization for the differentiation of clinically relevant staphylococci.". Paper presented in 12th International Symposium on Staphylococci & Staphylococcal Infections, 2006.
  92. Costa, Marta; Machado, Diana; Lencastre, Hermínia; Couto, Isabel. "Differentiation of staphylococci by checkerboard hybridization.". Paper presented in 12th International Congress on Infectious Diseases, 2006.
Journal article
  1. Schön, Thomas; Werngren, Jim; Machado, Diana; Borroni, Emanuele; Wijkander, Maria; Lina, Gerard; Mouton, Johan; et al. "Multicentre testing of the EUCAST broth microdilution reference method for MIC determination on Mycobacterium tuberculosis". Clinical Microbiology and Infection 27 2 (2021): 288.e1-288.e4. http://dx.doi.org/10.1016/j.cmi.2020.10.019.
    10.1016/j.cmi.2020.10.019
  2. Schön, Thomas; Werngren, Jim; Machado, Diana; Borroni, Emanuele; Wijkander, Maria; Lina, Gerard; Mouton, Johan; et al. "Antimicrobial susceptibility testing of Mycobacterium tuberculosis complex isolates – the EUCAST broth microdilution reference method for MIC determination". Clinical Microbiology and Infection 26 11 (2020): 1488-1492. http://dx.doi.org/10.1016/j.cmi.2020.07.036.
    10.1016/j.cmi.2020.07.036
  3. Borsoi, Ana Flávia; Paz, Josiane Delgado; Abbadi, Bruno Lopes; Macchi, Fernanda Souza; Sperotto, Nathalia; Pissinate, Kenia; Rambo, Raoní S.; et al. "Design, synthesis, and evaluation of new 2-(quinoline-4-yloxy)acetamide-based antituberculosis agents". European Journal of Medicinal Chemistry 192 (2020): 112179. http://dx.doi.org/10.1016/j.ejmech.2020.112179.
    10.1016/j.ejmech.2020.112179
  4. Perdigão, João; Gomes, Pedro; Miranda, Anabela; Maltez, Fernando; Machado, Diana; Silva, Carla; Phelan, Jody E.; et al. "Using genomics to understand the origin and dispersion of multidrug and extensively drug resistant tuberculosis in Portugal". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-59558-3.
    10.1038/s41598-020-59558-3
  5. Machado, Diana. "Detection of drug resistant Mycobacterium tuberculosis strains using Kit SIRE Nitratase: a multicenter study". Brazilian Archives of Biology and Technology (2020):
    In press
  6. Vianna, Júlia S.; Machado, Diana; Ramis, Ivy B.; Silva, Fábia P.; Bierhals, Dienefer V.; Abril, Michael Andrés; von Groll, Andrea; et al. "The Contribution of Efflux Pumps in Mycobacterium abscessus Complex Resistance to Clarithromycin". Antibiotics 8 3 (2019): 153. http://dx.doi.org/10.3390/antibiotics8030153.
    10.3390/antibiotics8030153
  7. Cá, Baltazar; Fonseca, Kaori L.; Sousa, Jeremy; Maceiras, Ana Raquel; Machado, Diana; Sanca, Lilica; Rabna, Paulo; et al. "Experimental Evidence for Limited in vivo Virulence of Mycobacterium africanum". Frontiers in Microbiology 10 (2019): http://dx.doi.org/10.3389/fmicb.2019.02102.
    10.3389/fmicb.2019.02102
  8. Phelan, Jody E.; O’Sullivan, Denise M.; Machado, Diana; Ramos, Jorge; Oppong, Yaa E. A.; Campino, Susana; O’Grady, Justin; et al. "Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs". Genome Medicine 11 1 (2019): http://dx.doi.org/10.1186/s13073-019-0650-x.
    10.1186/s13073-019-0650-x
  9. Rossi, Irene; Buttini, Francesca; Sonvico, Fabio; Affaticati, Filippo; Martinelli, Francesco; Annunziato, Giannamaria; Machado, Diana; et al. "Sodium Hyaluronate Nanocomposite Respirable Microparticles to Tackle Antibiotic Resistance with Potential Application in Treatment of Mycobacterial Pulmonary Infections". Pharmaceutics 11 5 (2019): 203. http://dx.doi.org/10.3390/pharmaceutics11050203.
    10.3390/pharmaceutics11050203
  10. Perdigão, João; Silva, Carla; Machado, Diana; Viveiros, Miguel; Portugal, Isabel. "CPLP-TB: a new tuberculosis transnational surveillance tool for the lusophone community". Anais do Instituto de Higiene e Medicina Tropical (2019): 87-90. https://doi.org/10.25761/anaisihmt.278.
  11. Oppong, Yaa E. A.; Phelan, Jody; Perdigão, João; Machado, Diana; Miranda, Anabela; Portugal, Isabel; Viveiros, Miguel; Clark, Taane G.; Hibberd, Martin L.. "Genome-wide analysis of Mycobacterium tuberculosis polymorphisms reveals lineage-specific associations with drug resistance". BMC Genomics 20 1 (2019): http://dx.doi.org/10.1186/s12864-019-5615-3.
    10.1186/s12864-019-5615-3
  12. Bouceiro-Mendes, R.; Ortins-Pina, A.; Marques, T.; Viveiros, M.; Machado, D.; Lito, L.; Ferreira, J.; et al. "A Case Report of Multibacillary Leprosy Presenting with Multiple Outbreaks of Erythema Nodosum Leprosum". Journal of the Portuguese Society of Dermatology and Venereology 77 1 (2019): 67-72. http://dx.doi.org/10.29021/spdv.77.1.1015.
    10.29021/spdv.77.1.1015
  13. Felicetti, Tommaso; Machado, Diana; Cannalire, Rolando; Astolfi, Andrea; Massari, Serena; Tabarrini, Oriana; Manfroni, Giuseppe; et al. "Modifications on C6 and C7 Positions of 3-Phenylquinolone Efflux Pump Inhibitors Led to Potent and Safe Antimycobacterial Treatment Adjuvants". ACS Infectious Diseases 5 6 (2019): 982-1000. http://dx.doi.org/10.1021/acsinfecdis.9b00041.
    10.1021/acsinfecdis.9b00041
  14. Machado, Diana. "Mycobacterium marinum lymphocutaneous infection.". Dermatol Online J 25 2 (2019): 10-14. https://www.ncbi.nlm.nih.gov/pubmed/30865411.
    Published
  15. Pena, Eduarda; Machado, Diana; Viveiros, Miguel; Jordão, Sofia. "A case report of disseminated Mycobacterium colombiense infection in an HIV patient". International Journal of Mycobacteriology 8 3 (2019): 295. http://dx.doi.org/10.4103/ijmy.ijmy_100_19.
    10.4103/ijmy.ijmy_100_19
  16. Pena, Eduarda; Machado, Diana; Viveiros, Miguel; Jordão, Sofia. "A case report of disseminated Mycobacterium colombiense infection in an HIV patient". International Journal of Mycobacteriology 8 3 (2019): 295. http://dx.doi.org/10.4103/ijmy.ijmy_100_19.
    10.4103/ijmy.ijmy_100_19
  17. Machado D; Couto I; Viveiros M. "Advances in the molecular diagnosis of tuberculosis: From probes to genomes.". Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (2018): http://europepmc.org/abstract/med/30508687.
    10.1016/j.meegid.2018.11.021
  18. Machado D; Azzali E; Couto I; Costantino G; Pieroni M; Viveiros M. "Adjuvant therapies against tuberculosis: discovery of a 2-aminothiazole targeting Mycobacterium tuberculosis energetics.". Future microbiology (2018): http://europepmc.org/abstract/med/30259757.
    10.2217/fmb-2018-0110
  19. Diana Machado; Miriam Girardini; Miguel Viveiros; Marco Pieroni. "Challenging the Drug-Likeness Dogma for New Drug Discovery in Tuberculosis". Frontiers in Microbiology 9 (2018): https://doi.org/10.3389%2Ffmicb.2018.01367.
    10.3389/fmicb.2018.01367
  20. Machado D; Antunes J; Simões A; Perdigão J; Couto I; McCusker M; Martins M; et al. "Contribution of efflux to colistin heteroresistance in a multidrug resistant Acinetobacter baumannii clinical isolate.". Journal of medical microbiology (2018): http://europepmc.org/abstract/med/29717972.
    10.1099/jmm.0.000741
  21. Deveson Lucas D; Crane B; Wright A; Han ML; Moffatt J; Bulach D; Gladman SL; et al. "Emergence of high-level colistin resistance in an Acinetobacter baumannii clinical isolate mediated by inactivation of the global regulator H-NS.". Antimicrobial agents and chemotherapy (2018): http://europepmc.org/abstract/med/29712662.
    10.1128/aac.02442-17
  22. Machado, Diana. "Efflux Activity Differentially Modulates the Levels of Isoniazid and Rifampicin Resistance among Multidrug Resistant and Monoresistant Mycobacterium tuberculosis Strains". Antibiotics (2018): http://www.mdpi.com/2079-6382/7/1/18.
    10.3390/antibiotics7010018
  23. Perdigão, João; Silva, Carla; Diniz J; Pereira C; Machado, Diana; Ramos J; Silva H; et al. "Clonal expansion across the seas as seen through CPLP-TB database: A joint effort in cataloguing Mycobacterium tuberculosis genetic diversity in Portuguese-speaking countries.". Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (2018): http://europepmc.org/abstract/med/29559379.
    10.1016/j.meegid.2018.03.011
  24. Phelan J; de Sessions PF; Tientcheu L; Perdigao J; Machado D; Hasan R; Hasan Z; et al. "Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally.". Scientific reports (2018): http://europepmc.org/abstract/med/29317751.
    10.1038/s41598-017-18188-y
  25. Diana Machado; Emmanuel Lecorche; Faiza Mougari; Emmanuelle Cambau; Miguel Viveiros. "Insights on Mycobacterium leprae Efflux Pumps and Their Implications in Drug Resistance and Virulence". Frontiers in Microbiology 9 (2018): https://doi.org/10.3389%2Ffmicb.2018.03072.
    10.3389/fmicb.2018.03072
  26. Machado, Diana. "Insights on the Mechanism of Action of INH-C10 as an Antitubercular Prodrug.". Molecular pharmaceutics (2017): http://europepmc.org/abstract/med/29091448.
    10.1021/acs.molpharmaceut.7b00719
  27. Machado, Diana. "Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance.". Antimicrobial agents and chemotherapy (2017): http://europepmc.org/abstract/med/28971867.
    10.1128/aac.01575-17
  28. Tavares, Ana Maria; Fronteira, Inês; Couto, Isabel; Machado, Diana; Viveiros, Miguel; Abecasis, Ana B.; Dias, Sónia. "HIV and tuberculosis co-infection among migrants in Europe: A systematic review on the prevalence, incidence and mortality". PLOS ONE 12 9 (2017): e0185526. http://dx.doi.org/10.1371/journal.pone.0185526.
    10.1371/journal.pone.0185526
  29. Machado, Diana. "Natural isoflavone biochanin A as a template for the design of new and potent 3-phenylquinolone efflux inhibitors against Mycobacterium avium.". European journal of medicinal chemistry (2017): http://europepmc.org/abstract/med/28964936.
    10.1016/j.ejmech.2017.09.014
  30. Machado, Diana. "Substituted N-Phenyl-5-(2-(phenylamino)thiazol-4-yl)isoxazole-3-carboxamides Are Valuable Antitubercular Candidates that Evade Innate Efflux Machinery.". Journal of medicinal chemistry (2017): http://europepmc.org/abstract/med/28749666.
    10.1021/acs.jmedchem.7b00793
  31. Machado D; Coelho TS; Perdigão J; Pereira C; Couto I; Portugal I; Maschmann RA; et al. "Interplay between Mutations and Efflux in Drug Resistant Clinical Isolates of Mycobacterium tuberculosis.". (2017): http://europepmc.org/abstract/med/28496433.
    10.3389/fmicb.2017.00711
  32. Machado D; Fernandes L; Costa SS; Cannalire R; Manfroni G; Tabarrini O; Couto I; Sabatini S; Viveiros M. "Mode of action of the 2-phenylquinoline efflux inhibitor PQQ4R against Escherichia coli.". (2017): http://europepmc.org/abstract/med/28516003.
    10.7717/peerj.3168
  33. Perdigão, J.; Clemente, S.; Ramos, J.; Masakidi, P.; Machado, D.; Silva, C.; Couto, I.; et al. "Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola". Scientific Reports 7 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85013662122&partnerID=MN8TOARS.
    10.1038/srep42814
  34. Machado, Diana. "HIV and tuberculosis co-infection among migrants in Europe: A systematic review on the prevalence, incidence and mortality.". PloS one (2017): http://europepmc.org/abstract/med/28957400.
  35. Machado, Diana; Viveiros, Miguel. "Tuberculose extensivamente resistente: uma realidade presente. Revisão da literatura a propósito de um caso clínico". Revista Portuguesa de Doenças Infecciosas 13 (2017): 24-34.
  36. Perdigao, Joao; Clemente, Sofia; Ramos, Jorge; Masakidi, Pedro; Machado, Diana; Silva, Carla; Couto, Isabel; et al. "Genetic diversity, transmission dynamics, and drug resistance of Mycobacterium tuberculosis in Luanda, Angola.". International journal of mycobacteriology 5 Suppl 1 (2016): S38-S39. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:28043599&KeyUID=MEDLINE:28043599.
    10.1016/j.ijmyco.2016.09.050
  37. Perdigao, Joao; Maltez, Fernando; Machado, Diana; Silva, Hugo; Pereira, Catarina; Silva, Carla; Couto, Isabel; Viveiros, Miguel; Portugal, Isabel. "Beyond extensively drug-resistant tuberculosis in Lisbon, Portugal: a case of linezolid resistance acquisition presenting as an iliopsoas abscess". International Journal of Antimicrobial Agents 48 5 (2016): 569-570. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000389519000017&KeyUID=WOS:000389519000017.
    10.1016/j.ijantimicag.2016.07.026
  38. Costa, Sofia Santos; Lopes, Elizeth; Azzali, Elisa; Machado, Diana; Coelho, Tatiane; Almeida da Silva, Pedro Eduardo; Viveiros, Miguel; Pieroni, Marco; Couto, Isabel. "An Experimental Model for the Rapid Screening of Compounds with Potential Use Against Mycobacteria". Assay and Drug Development Technologies 14 9 (2016): 524-534. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000388119600002&KeyUID=WOS:000388119600002.
    10.1089/adt.2016.752
  39. Machado D; Pires D; Perdigão J; Couto I; Portugal I; Martins M; Amaral L; Anes E; Viveiros M. "Ion Channel Blockers as Antimicrobial Agents, Efflux Inhibitors, and Enhancers of Macrophage Killing Activity against Drug Resistant Mycobacterium tuberculosis.". (2016): http://europepmc.org/abstract/med/26919135.
    10.1371/journal.pone.0149326
  40. Phelan, Jody; O'Sullivan, Denise M.; Machado, Diana; Ramos, Jorge; Whale, Alexandra S.; O'Grady, Justin; Dheda, Keertan; et al. "The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs". Genome Medicine 8 (2016): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000390284000001&KeyUID=WOS:000390284000001.
    10.1186/s13073-016-0385-x
  41. Pieroni M; Machado D; Azzali E; Santos Costa S; Couto I; Costantino G; Viveiros M. "Rational Design and Synthesis of Thioridazine Analogues as Enhancers of the Antituberculosis Therapy.". (2015): http://europepmc.org/abstract/med/26197353.
    10.1021/acs.jmedchem.5b00428
  42. Diana Machado; Rolando Cannalire; Sofia Santos Costa; Giuseppe Manfroni; Oriana Tabarrini; Violetta Cecchetti; Isabel Couto; Miguel Viveiros; Stefano Sabatini. "Boosting Effect of 2-Phenylquinoline Efflux Inhibitors in Combination with Macrolides against Mycobacterium smegmatis and Mycobacterium avium". ACS Infect. Dis. (2015): 150723073310003-150723073310003. http://dx.doi.org/10.1021/acsinfecdis.5b00052.
    10.1021/acsinfecdis.5b00052
  43. Cambau E; Viveiros M; Machado D; Raskine L; Ritter C; Tortoli E; Matthys V; et al. "Revisiting susceptibility testing in MDR-TB by a standardized quantitative phenotypic assessment in a European multicentre study.". (2015): http://europepmc.org/abstract/med/25587993.
    10.1093/jac/dku438
  44. João Perdigão; Hugo Silva; Diana Machado; Rita Macedo; Fernando Maltez; Carla Silva; Luisa Jordao; et al. "Genomic diversity of drug-resistant Mycobacterium tuberculosis isolates in Lisbon Portugal: Towards tuberculosis genomic epidemiology". International Journal of Mycobacteriology 4 (2015): 27-28. https://doi.org/10.1016%2Fj.ijmyco.2015.01.001.
    10.1016/j.ijmyco.2015.01.001
  45. Rabna P; Ramos J; Ponce G; Sanca L; Mané M; Armada A; Machado D; et al. "Direct Detection by the Xpert MTB/RIF Assay and Characterization of Multi and Poly Drug-Resistant Tuberculosis in Guinea-Bissau, West Africa.". (2015): http://europepmc.org/abstract/med/26017968.
    10.1371/journal.pone.0127536
  46. Coelho T; Machado D; Couto I; Maschmann R; Ramos D; von Groll A; Rossetti ML; Silva PA; Viveiros M. "Enhancement of antibiotic activity by efflux inhibitors against multidrug resistant Mycobacterium tuberculosis clinical isolates from Brazil.". (2015): http://europepmc.org/abstract/med/25972842.
    10.3389/fmicb.2015.00330
  47. Perdigão J; Silva H; Machado D; Macedo R; Maltez F; Silva C; Jordao L; et al. "Unraveling Mycobacterium tuberculosis genomic diversity and evolution in Lisbon, Portugal, a highly drug resistant setting.". (2014): http://europepmc.org/abstract/med/25407810.
  48. Costa P; Amaro A; Ferreira AS; Machado D; Albuquerque T; Couto I; Botelho A; Viveiros M; Inácio J. "Rapid identification of veterinary-relevant Mycobacterium tuberculosis complex species using 16S rDNA, IS6110 and Regions of Difference-targeted dual-labelled hydrolysis probes.". (2014): http://europepmc.org/abstract/med/25192844.
    10.1016/j.mimet.2014.08.017
  49. Machado, Diana; Ramos, Jorge; Couto, Isabel; Cadir, Nureisha; Narciso, Inacio; Coelho, Elizabeth; Viegas, Sofia; Viveiros, Miguel. "Assessment of the BD MGIT TBc Identification Test for the Detection of Mycobacterium tuberculosis Complex in a Network of Mycobacteriology Laboratories". Biomed Research International (2014):
    10.1155/2014/398108
  50. Leao, Celia; Canto, Ana; Machado, Diana; Sanches, Ilda Santos; Couto, Isabel; Viveiros, Miguel; Inacio, Joao; Botelho, Ana. "Relatedness of Mycobacterium avium subspecies hominissuis clinical isolates of human and porcine origins assessed by MLVA.". Veterinary microbiology 173 1-2 (2014): 92-100.
    10.1016/j.vetmic.2014.06.027
  51. Pedrosa, Pedro; Veigas, Bruno; Machado, Diana; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro V.. "Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis". Tuberculosis 94 3 (2014): 332-337.
    10.1016/j.tube.2013.12.009
  52. Perdigao, J.; Macedo, R.; Machado, D.; Silva, C.; Jordao, L.; Couto, I.; Viveiros, M.; Portugal, I.. "GidB mutation as a phylogenetic marker for Q1 cluster Mycobacterium tuberculosis isolates and intermediate-level streptomycin resistance determinant in Lisbon, Portugal". Clinical Microbiology and Infection 20 5 (2014): O278-O284.
    10.1111/1469-0691.12392
  53. Machado, Diana; Ramos, Jorge; Couto, Isabel; Cadir, Nureisha; Narciso, Inácio; Coelho, Elizabeth; Viegas, Sofia; Viveiros, Miguel. "Assessment of the BD MGIT TBc Identification Test for the Detection of Mycobacterium tuberculosisComplex in a Network of Mycobacteriology Laboratories". BioMed Research International 2014 (2014): 1-6. http://dx.doi.org/10.1155/2014/398108.
    10.1155/2014/398108
  54. Armada, Ana; Alexandru, Tatiana; Machado, Diana; Danko, Balazs; Hunyadi, Attila; Dinache, Andra; Nastasa, Viorel; et al. "The in vitro activity of products formed from exposure of chlorpromazine to a 266 nm laser beam against species of mycobacteria of human interest". In Vivo 27 5 (2013): 605-610.
    Open access • Published
  55. Costa, Sofia Santos; Falcao, Celeste; Viveiros, Miguel; Machado, Diana; Martins, Marta; Melo-Cristino, Jose; Amaral, Leonard; Couto, Isabel. "Exploring the contribution of efflux on the resistance to fluoroquinolones in clinical isolates of Staphylococcus aureus (vol 11, pg 241, 2011)". Bmc Microbiology 13 (2013):
    10.1186/1471-2180-13-127
  56. Machado, Diana; Perdigao, Joao; Ramos, Jorge; Couto, Isabel; Portugal, Isabel; Ritter, Claudia; Boettger, Erik C.; Viveiros, Miguel. "High-level resistance to isoniazid and ethionamide in multidrug-resistant Mycobacterium tuberculosis of the Lisboa family is associated with inhA double mutations". Journal of Antimicrobial Chemotherapy 68 8 (2013): 1728-1732.
    10.1093/jac/dkt090
  57. Perdigao, Joao; Macedo, Rita; Silva, Carla; Machado, Diana; Couto, Isabel; Viveiros, Miguel; Jordao, Luisa; Portugal, Isabel. "From multidrug-resistant to extensively drug-resistant tuberculosis in Lisbon, Portugal: the stepwise mode of resistance acquisition". Journal of Antimicrobial Chemotherapy 68 1 (2013): 27-33.
    10.1093/jac/dks371
  58. Viveiros, Miguel; Martins, Marta; Rodrigues, Liliana; Machado, Diana; Couto, Isabel; Ainsa, Jose; Amaral, Leonard. "Inhibitors of mycobacterial efflux pumps as potential boosters for anti-tubercular drugs". Expert Review of Anti-Infective Therapy 10 9 (2012): 983-998.
    10.1586/ERI.12.89
  59. Machado, Diana; Couto, Isabel; Perdigao, Joao; Rodrigues, Liliana; Portugal, Isabel; Baptista, Pedro; Veigas, Bruno; Amaral, Leonard; Viveiros, Miguel. "Contribution of Efflux to the Emergence of Isoniazid and Multidrug Resistance in Mycobacterium tuberculosis". Plos One 7 4 (2012):
    10.1371/journal.pone.0034538
  60. Rodrigues, Liliana; Machado, Diana; Couto, Isabel; Amaral, Leonard; Viveiros, Miguel. "Contribution of efflux activity to isoniazid resistance in the Mycobacterium tuberculosis complex". Infection Genetics and Evolution 12 4 (2012): 695-700.
    10.1016/j.meegid.2011.08.009
  61. Costa, Sofia Santos; Falcao, Celeste; Viveiros, Miguel; Machado, Diana; Martins, Marta; Melo-Cristino, Jose; Amaral, Leonard; Couto, Isabel. "Exploring the contribution of efflux on the resistance to fluoroquinolones in clinical isolates of Staphylococcus aureus". Bmc Microbiology 11 (2011):
    10.1186/1471-2180-11-241
  62. Veigas, Bruno; Machado, Diana; Perdigao, Joao; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro V.. "Au-nanoprobes for detection of SNPs associated with antibiotic resistance in Mycobacterium tuberculosis". Nanotechnology 21 41 (2010):
    10.1088/0957-4484/21/41/415101
  63. Viveiros, Miguel; Martins, Marta; Couto, Isabel; Rodrigues, Liliana; Machado, Diana; Portugal, Isabel; Amaral, Leonard. "Molecular tools for rapid identification and novel effective therapy against MDRTB/XDRTB infections". Expert Review of Anti-Infective Therapy 8 4 (2010): 465-480.
    10.1586/eri.10.20
  64. Couto, I.; Machado, D.; Viveiros, M.; Rodrigues, L.; Amaral, L.. "Identification of nontuberculous mycobacteria in clinical samples using molecular methods: a 3-year study". Clinical Microbiology and Infection 16 8 (2010): 1162-1164.
    10.1111/j.1469-0691.2009.03076.x
  65. Rodrigues, Liliana; Sampaio, Daniela; Couto, Isabel; Machado, Diana; Kern, Winfried V.; Amaral, Leonard; Viveiros, Miguel. "The role of efflux pumps in macrolide resistance in Mycobacterium avium complex". International Journal of Antimicrobial Agents 34 6 (2009): 529-533.
    10.1016/j.ijantimicag.2009.07.010
Manual
  1. Machado, Diana. Manual de segurança biológica (2014).. Portugal: Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical. 2014.
  2. Machado, Diana. Programa de treino em Micobacteriologia. Portugal: Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical. 2011.
Thesis / Dissertation
  1. Machado, Diana. "The dynamics of drug resistance in Mycobacterium tuberculosis: exploring the biological basis of multi- and extensively drug resistant tuberculosis (MDR/XDRTB) as a route for alternative therapeutic strategies". PhD, 2014.
  2. Machado, Diana. "Dinâmica fisiológica e mutacional da multirresistência em Mycobacterium tuberculosis". Master, Universidade Nova de Lisboa, 2009.
  3. Machado, Diana. "Aplicação de “checkerboard hybridization” para identificação de Staphylococcus". Degree, Universidade Lusófona de Humanidades e Tecnologias, 2006.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2020/04/18 Colistin heteroresistance in carbapenemase-producing Acinetobacter baumannii 30th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)
European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) (Paris, France)
2020/01/31 Development of lipophilic efflux inhibitors as adjuvants of Mycobacterium tuberculosis chemotherapy II Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal
LisbonPH, Faculdade de Farmácia da Universidade de Lisboa (Lisboa, Portugal)
2019/12/05 Insights on Acinetobacter baumannii heteroresistance to colistin: what is the fate? Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19)
Congresso Nacional de Microbiologia e Biotecnologia (MicroBiotec19) (Coimbra, Portugal)
2019/11/21 Colistin heteroresistance in Acinetobacter baumannii: an unexpected cause of treatment failure Third GHTM Antimicrobial Resistance Awareness Day
GHTM, Instituto de Higiene e Medicina Tropical (Lisboa, Portugal)
2019/10/30 Pretomanid susceptibility testing of Mycobacterium tuberculosis complex isolates using the BACTECTM MGITTM 960 system. 50th World Conference on Lung Health of the International Union Against Tuberculosis and Lung Disease (The Union)
Lung Health of the International Union Against Tuberculosis and Lung Disease (Hyderabad, India)
2019/09/30 Instituto de Higiene e Medicina Tropical: a avaliação de risco na nossa instituição. 1º Seminário Lab-PTBioNet
Rede Laboratorial Portuguesa de Biossegurança (Lisboa, Portugal)
2019/06/30 The contribution of membrane transporter proteins to extensively-drug resistant phenotypes in Acinetobacter baumannii Australian Society for Microbiology Annual Scientific Meeting
Australian Society for Microbiology (Adelaide, Australia)
2019/06/30 Targeting Mycobacterium tuberculosis with lipophilic efflux inhibitors exploring their dual activity: dissipation of the proton motive force and efflux inhibition 40th Annual Congress of the European Society of Mycobacteriology
European Society of Mycobacteriology (Valencia, Spain)
2019/04/13 Exploring the phenotypic and genetic basis of drug-resistant Mycobacterium tuberculosis strains in Lisbon, Portugal. 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)
European Society of Clinical Microbiology and Infectious Diseases (ESCMID) (Amesterdão, Netherlands)
2019/04/13 Contribution of ß-lactamases, porins and efflux pumps to carbapenem resistance in Acinetobacter baumannii. 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)
European Society of Clinical Microbiology and Infectious Diseases (ESCMID) (Amestredão, Netherlands)
2019/04/13 Towards a new reference drug susceptibility testing method for Mycobacterium tuberculosis. 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)
European Society of Clinical Microbiology and Infectious Diseases (ESCMID) (Amsterdão, Netherlands)
2019/04/10 Epidemiologia molecular da resistência em Mycobacterium tuberculosis multirresistente em Lisboa. 4o Congresso Nacional de Medicina Tropical
Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa (Lisboa, Portugal)
2018/04/21 Contribution of efflux to colistin heteroresistance in a multidrug resistant Acinetobacter baumannii clinical isolate. 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)
European Society of Clinical Microbiology and Infectious Diseases (ESCMID) (Madrid, Spain)
2018/04/21 Evaluation of the performance of MinION sequencing to identify multi- and extensively drug resistant tuberculosis. 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)
European Society of Clinical Microbiology and Infectious Diseases (ESCMID) (Madrid, Spain)
2017/10/02 The contribution of efflux pumps and porins in Acinetobacter baumannii resistance to carbapenems 3rd NOVAHealth Genetics Workshop
Universidade NOVA de Lisboa (Lisboa, Portugal)
2017/07 Genetic diversity and mapping of M. tuberculosis strains in Portuguese Speaking Countries. VI National Workshop – REDE TB
Rede Brasileira de Pesquisas em Tuberculose (REDE-TB, Brazilian Tuberculosis Research Network) (Rio de Janeiro, Brazil)
2017/06/11 Tracking Mycobacterium tuberculosis across the seas: CPLP-TB and genetic diversity in the Lusophone space. Translational Research and Innovation in Human and Health Sciences – 2nd International Congress of CiiEM
Egas Moniz - Cooperativa de Ensino Superior
2017/04/19 Tuberculosis across the seas: CPLP-TB - a joint effort in cataloguing Mycobacterium tuberculosis genetic diversity in the lusophone space. 3o Congresso Nacional de Medicina Tropical
Instituto de Higiene e Medicina Tropical (Lisboa, Portugal)
2017/02/25 Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Luanda, Angola. 2nd Asian-African Congress of Mycobacteriology
Asian-African Society of Mycobacteriology (AASM) (Isfahan, Iran)
2016/09/18 Genome downsizing and drug resistance in Mycobacterium leprae: are there places for drug efflux pumps? 19th International Leprosy Congress
The China Leprosy Association and the China Association for Science and Technology (Beijing, China)
2016/04/08 Antimycobacterial and efflux inhibitory activity of 2-aminothiazole lead compound against Mycobacterium tuberculosis. 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID)
European Society of Clinical Microbiology and Infectious Diseases (ESCMID) (Amesterdam, Netherlands)
2015/12/10 Mycobacterium smegmatis model for the rapid screening of new compounds with efflux inhibitory and antimycobacterial activity. MicroBiotec15
Sociedade Portuguesa de Microbiologia e Sociedade Portuguesa de Biotecnologia (Évora, Portugal)
2014/06/29 Efflux inhibitors against drug resistant Mycobacterium tuberculosis: antimicrobial agents and enhancers of macrophage killing activity. 35th Annual Congress of the European Society of Mycobacteriology.
European Society of Mycobacteriology (Vienna, Austria)
2013/12/13 Actividade antimicobacteriana de compostos inibidores de efluxo contra Mycobacterium tuberculosis resistente aos antibacilares. Jornadas Científicas do Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa
Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (Lisboa, Portugal)
2013/12/06 Efflux inhibitors against drug resistant Mycobacterium tuberculosis: antimicrobial agents and enhancers of macrophage killing activity. MicroBiotec13
Sociedade Portuguesa de Microbiologia e pela Sociedade Portuguesa de Biotecnologia (Aveiro, Portugal)
2013/11/04 Estética y MNT. Un problema emergente en Portugal. XVII Taller Internacional sobre Tuberculosis
UITB - Universidad de Investigación en Tuberculosis de Barcelona, TB Investigation Unit of Barcelona (Barcelona, Spain)
2013/06/30 Anti-tuberculosis activity of efflux inhibitors against drug resistant Mycobacterium tuberculosis. 34th Annual Congress of the European Society of Mycobacteriology
European Society of Mycobacteriology (Florence, Italy)
2013/04/21 Detecção directa da TB-MR através do teste Xpert MTB/RIF na Guiné-Bissau. 2º Congresso Nacional de Medicina Tropical: Workshop “Multidrug-resistant tuberculosis”
Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa (Lisboa, Portugal)
2013/04/21 Detecção de MDRTB por gold-nanoprobes – uma nova abordagem tecnológica desenvolvida em Portugal. 2º Congresso Nacional de Medicina Tropical: Workshop “Multidrug-resistant tuberculosis”.
Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa (Lisboa, Portugal)
2013/04/21 Detecção directa de MDRTB em Portugal: métodos e aplicações comerciais. A experiência do IHMT. 2º Congresso Nacional de Medicina Tropical: Workshop “Multidrug-resistant tuberculosis”.
Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa (Lisboa, Portugal)
2013/04/20 Inibidores de efluxo como adjuvantes no tratamento da tuberculose resistente aos antibacilares. 2o Congresso Nacional de Medicina Tropical
Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa (Lisboa, Portugal)
2013/03/26 Significance of efflux in multidrug resistance in Mycobacterium tuberculosis. GRConference: multi-drug efflux systems shared molecular mechanisms but diverging roles in physiology and medicine. 2013 Gordon Research Conference on Multi-Drug Efflux Systems, GRG session: significance of efflux pumps in etiology of drug resistance.
Gordon Research Conference (California, United States)
2012/12/12 Resistência de alto nível à isoniazida e etionamida em estirpes de Mycobacterium tuberculosis multirresistentes está associada a mutações duplas no gene inhA. Jornadas Científicas do Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa
Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (Lisboa, Portugal)
2012/10/28 O teste de susceptibilidade semi-quantitativo de 1ª e 2ª linha para Mycobacterium tuberculosis usando o sistema BD EpiCenter™ TB-eXiST: um importante contributo para a Luta Contra a Tuberculose Resistente aos antibióticos. XXI Congresso Latino Americano de Microbiologia (XXI ALAM) and XIV Simpósio Brasileiro de Micobactérias
Sociedade Brasileira de Microbiologia (SBM) e Associação Latinoamericana de Microbiologia (ALAM) (São Paulo, Brazil)
2012/07/17 High level resistance to isoniazid and ethionamide among multidrug resistant Mycobacterium tuberculosis strains from Lisbon, Portugal, is associated with double mutations in inhA gene. Workshop “Mycobacterium tuberculosis”
Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa (Lisboa, Portugal)
2010/07/04 Mutational and physiological dynamic of drug resistance in Mycobacterium tuberculosis. 31th Annual Congress of the European Society of Mycobacteriology
European Society of Mycobacteriology (Bled, Slovenia)
2010/07/04 Importance of efflux systems on the resistance to fluoroquinolones in Staphylococcus aureus. COST ACTION BM0701 Meeting
Cost Action (Bremen, Germany)
2009/07/05 Contribution of efflux pump activity for macrolide resistance in M. avium complex. 30th Annual Congress of the European Society of Mycobacteriology
European Society of Mycobacteriology (Porto, Portugal)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2020 - Current Estudo de competição entre substratos de bombas de efluxo em Escherichia coli por fluorimetria em tempo real usando Vermelho do Nilo
Supervisor of Inês Maia
Biomedical Sciences (Master)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019/08 - Current Insights on Acinetobacter baumannii heteroresistance to colistin: what is the fate?
Supervisor
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019/08 - Current The dark-side of drug resistance in Escherichia coli: efflux pumps and ß-lactamase producing strains.
Supervisor
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019/08 - Current Exploring the contribution of efflux on the resistance to antibiotics, biocides and dyes in clinical isolates of Escherichia coli.
Supervisor
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019/08 - Current Evaluation of competition between substrates of the efflux pump system AcrAB-TolC of Escherichia coli using Red Nile.
Supervisor
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019 - Current Lepra em Angola: caracterização socio-epidemiológica e genotipagem de Mycobacterium leprae
Co-supervisor
Biomedical Sciences (PhD)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019 - Current GeneXpert MTB/RIF no diagnóstico da tuberculose em São Tomé e Príncipe: avaliação da implementação.
Co-supervisor
Public Health and Development (Master)
2018 - Current O papel dos mutantes hip na resistência aos antibióticos mediada por efluxo em bactérias persistentes.
Supervisor
Medical Microbiology (Master)
Universidade Nova de Lisboa, Portugal
2017 - Current Aplicação de uma técnica de MAS-PCR para a detecção de tuberculose multi- e extensivamente resistente no Núcleo de Tuberculose e Micobacterioses do Instituto Adolfo Lutz, Laboratório de Referência de São Paulo para a Tuberculose (São Paulo, Brasil): validação fenotípica e molecular.
Co-supervisor
Estágio de Doutorado CAPES – Programa PVE (PhD)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2018 - 2020 Detecção e inibição da actividade de ß-lactamases em isolados clinicos de Escherichia coli combinando inibidores de ß-lactamases e inibidores de efluxo usando o método MTT: validação fenotípica e genotípica.
Supervisor
Medical Microbiology (Master)
Universidade Nova de Lisboa, Portugal
2018 - 2019 Uma abordagem bioinformática para o estudo da contribuição das bombas de efluxo “Small Multidrug Resistance” na resistência aos antibióticos em Acinetobacter baumannii através de análise de genoma completo.
Co-supervisor
Medical Microbiology (Master)
Universidade Nova de Lisboa, Portugal
2017 - 2019 Caracterização molecular e sequenciamento de isolados de Mycobacterium tuberculosis na fronteira de Roraima, Brasil.
Co-supervisor
Programa de Pós-Graduação em Ciências da Saúde – Programa PVE (PhD)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2017 - 2018 Caracterização da actividade de efluxo de Hoechst 33258 em Escherichia coli.
Supervisor
Biomedical Sciences (Master)
Universidade Nova de Lisboa, Portugal
2017 - 2018 Caracterização dos mecanismos de resistência à colistina em isolados clínicos de Acinetobacter baumannii resistentes aos carbapenemos.
Supervisor
Biomedical Sciences (Master)
Universidade Nova de Lisboa, Portugal
2016 - 2017 A contribuição do efluxo na resistência aos ß-lactâmicos em isolados clínicos de Escherichia coli.
Co-supervisor
Medical Microbiology (Master)
Universidade Nova de Lisboa, Portugal
2016 - 2017 Estudo da contribuição de bombas de efluxo, porinas e ß-lactamases na resistência aos carbapenemos em isolados clínicos de Acinetobacter baumannii.
Co-supervisor
Medical Microbiology (Master)
Universidade Nova de Lisboa, Portugal
2016 - 2017 Contribuição do efluxo para a emergência da resistência em bactérias persistentes.
Co-supervisor
Biomedical Sciences (Master)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2016 - 2017 Aplicação de um método fluorométrico em placa de 96 poços para a detecção de actividade de efluxo em bactérias.
Co-supervisor
Biomedical Sciences (Master)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2014 - 2016 Contribuição do efluxo para a aquisição de resistência aos antibióticos em isolados clínicos de Acinetobacter baumannii.
Co-supervisor
Medical Microbiology (Master)
Universidade Nova de Lisboa, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2020/10/09 - 2020/10/09 Attended the III International Conference, NOVAhealth Chronic Disease and Infection, held at Universidade NOVA de Lisboa, Portugal.
Conference
III International Conference NOVAhealth Chronic Disease and Infection "Infection, Cancer and Global Health"
Universidade Nova de Lisboa, Portugal
2020/02/04 - 2020/02/04 EUCAST Antimicrobial Susceptibility Testing Workshop
Workshop
EUCAST Antimicrobial Susceptibility Testing Workshop
ESCMID Study Group for Mycobacterial Infections - ESGMYC, Switzerland
2020/01/31 - 2020/01/31 II Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal
Symposium
II Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal
2019/11/21 - 2019/11/21 GHTM special session: “Third GHTM Antimicrobial Resistance Awareness Day”
Workshop
GHTM special session: “Third GHTM Antimicrobial Resistance Awareness Day”
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019/11/13 - 2019/11/13 VIII Workshop Biossegurança: "Cenários em situações de emergência de origem biológica"
Workshop
VIII Workshop Biossegurança: "Cenários em situações de emergência de origem biológica"
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
2019/07/08 - 2019/07/10 Encontro Ciência ´19
Conference
Encontro Ciência ´19
Fundação para a Ciência e a Tecnologia, I.P., Portugal
2019/03/25 - 2019/03/25 I Simpósio Dia Mundial da Tuberculose
Symposium
I Simpósio Dia Mundial da Tuberculose
Universidade de Lisboa Faculdade de Farmácia, Portugal
2019/03/21 - 2019/03/21 NOVAsaúde IV Genetics Workshop
Workshop
NOVAsaúde IV Genetics Workshop
Universidade Nova de Lisboa, Portugal
2018/11/13 - 2018/11/13 I International Conference NOVAhealth on Chronic Disease and Infection. Resistance to antibiotics: from prevention to control of infection in “One Health”.
Conference
I International Conference NOVAhealth on Chronic Disease and Infection. Resistance to antibiotics: from prevention to control of infection in “One Health”
Universidade Nova de Lisboa, Portugal
2018/11/13 - 2018/11/13 GHTM special session “Second GHTM Antimicrobial Resistance Awareness Day”
Other
GHTM special session “Second GHTM Antimicrobial Resistance Awareness Day”
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2018/07/02 - 2018/07/04 Encontro Ciência ´18
Meeting
Encontro Ciência ´18
2018/06/18 - 2018/06/18 I Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal. Attendee
Symposium
I Simpósio de Investigação em Tuberculose e Micobactérias Não Tuberculosas em Portugal
Universidade do Porto Instituto de Saúde Pública, Portugal
2017/11/15 - 2017/11/15 GHTM special session “First GHTM Antimicrobial Resistance Awareness Day”
Other
GHTM special session “First GHTM Antimicrobial Resistance Awareness Day”
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2017/10/02 - 2017/10/02 NOVAsaúde III Genetics Workshop. Attendee
Workshop
NOVAsaúde III Genetics Workshop
Universidade Nova de Lisboa, Portugal
2017/07/03 - 2017/07/05 Encontro Ciência ´17 attendee
Meeting
Encontro Ciência ´17
2017/03/24 - 2017/03/24 Simpósio “Tuberculose: a história e o património”. attendee
Symposium
Simpósio “Tuberculose: a história e o património”
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2016/07/04 - 2016/07/06 Encontro Ciência ´16. attendee
Meeting
Encontro Ciência ´16
2014/11/20 - 2014/11/21 Host-pathogen interaction, towards a personalized medicine. attendee
Conference
Cycle of conferences. Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2014/06/18 - 2014/06/18 Lisbon workshop on new strategies to fight tuberculosis.
Workshop
Lisbon workshop on new strategies to fight tuberculosis
Universidade de Lisboa Faculdade de Farmácia, Portugal
2013/04/24 - 2013/04/26 Genomics workshop – Lectured by Thomas Otto, Sanger Centre.
Workshop
Genomics workshop – Lectured by Thomas Otto, Sanger Centre
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2011/06/21 - 2011/06/21 Phage and mycobacterial molecular biology workshop
Workshop
Phage and mycobacterial molecular biology workshop
Universidade de Lisboa Faculdade de Farmácia, Portugal
2010/12/01 - 2010/12/03 ESF EMRC Exploratory Workshop EW09-007: “Multidisciplinary consortium for the development of effective, but non-toxic drugs against MDR-TB and XDR-TB”
Workshop
ESF EMRC Exploratory Workshop EW09-007: “Multidisciplinary consortium for the development of effective, but non-toxic drugs against MDR-TB and XDR-TB”
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2009/04/08 - 2009/04/08 URIA Mycobacterium Workshop
Workshop
URIA Mycobacterium Workshop
Universidade de Lisboa Faculdade de Farmácia, Portugal
2008/05/14 - 2008/05/14 Real Time PCR seminary: New tools for gene expression
Seminar
Real Time PCR seminary: New tools for gene expression
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2008/03/18 - 2008/03/18 2as Jornadas de Actualização de PCR em Tempo Real.
Other
2as Jornadas de Actualização de PCR em Tempo Real.
Instituto de Medicina Molecular João Lobo Antunes, Portugal
2006/06/28 - 2006/06/28 Reunião de actualização em Tuberculose
Meeting
Reunião de actualização em Tuberculose
Becton Dickinson AB, Sweden

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2020 - Current Curricular Unit “Impact of the Disease and its Determinants”, PhD in Tropical Diseases and Global Health (IHMT/UNL) Tropical Diseases and Global Health (Doutoramento) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2020 - Current Curricular Unit “Rotation Theme I”, PhD in Tropical Diseases and Global Health (IHMT/UNL) Tropical Diseases and Global Health (Doutoramento) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2020 - Current Curricular Unit “Rotation Theme II”, PhD in Tropical Diseases and Global Health (IHMT/UNL) Tropical Diseases and Global Health (Doutoramento) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019/06 - Current Advanced training on the laboratory diagnosis of tuberculosis of clinical pathologists from the Laboratório de Microbiologia do Serviço de Patologia Clínica, Hospital Nossa Senhora do Rosário, Centro Hospitalar Barreiro/Montijo. Coordination: Miguel Viveiros | Diana Machado. (Outros)
2019 - Current Curricular Unit “Challenges and Perspectives in Biology of Microorganisms”, PhD in Biomedical Sciences (IHMT/UNL) Biomedical Sciences (Doutoramento) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2017 - Current Curricular Unit “Tuberculosis”, PhD in Biomedical Sciences (IHMT/UNL) Biomedical Sciences (Doutoramento) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2017 - Current Curricular Unit “Medical Bacteriology”, PhD in Biomedical Sciences (IHMT/UNL) Biomedical Sciences (Doutoramento) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2017 - Current Curricular Unit “Molecular Epidemiology of Infectious and Parasitic Diseases”, PhD in Biomedical Sciences (IHMT/UNL) Biomedical Sciences (Doutoramento) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2014/04 - Current Advanced training on the laboratory diagnosis of tuberculosis of clinical pathologists from the Laboratório de Microbiologia Clínica e Biologia Molecular do Serviço de Patologia Clínica, Hospital de Egas Moniz, Centro Hospitalar de Lisboa Ocidental. Coordination: Miguel Viveiros. (Outros) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2010 - Current Curricular Unit of Tuberculosis and other Mycobacterioses, Master in Medical Microbiology Medical Microbiology (Mestrado) Universidade Nova de Lisboa, Portugal
2010 - Current Curricular Unit of Medical Bacteriology, Master in Medical Microbiology (UNL) Medical Microbiology (Mestrado) Universidade Nova de Lisboa, Portugal
2008/04 - Current Strategic plan for the control of tuberculosis in Mozambique, Angola, Guiné-Bissau and S. Tomé e Principe. Training program in Mycobacteriology. Coordination: Miguel Viveiros. Instituto de Higiene e Medicina Tropical. Supported by Fundação Calouste Gulbenkian until 2012. (Outros) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2007 - Current Curricular Unit of Tuberculosis and other Mycobacterioses, Master in Biomedical Sciences (IHMT/UNL) Biomedical Sciences (Mestrado) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2014/04 - 2019/12 Advanced training on the laboratory diagnosis of tuberculosis of clinical pathologists from the Laboratório de Microbiologia do Serviço de Patologia Clínica, IPO Lisboa Francisco Gentil. Coordination: Miguel Viveiros. (Outros) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2010 - 2012 Curricular Unit of Molecular Methods of Diagnosis in Bacteriology and Mycology, • Master in Medical Microbiology (UNL) Medical Microbiology (Mestrado) Universidade Nova de Lisboa, Portugal
2010 - 2012 Curricular Unit of Clinical Microbiology, Master in Molecular Genetics and Biomedicine (FCT/UNL) Molecular Genetics and Biomedicine (Mestrado) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2011/07/01 - 2011/07/31 Training program in Mycobacteriology. Video-conference. Lisboa – Luanda. “Tuberculose: prevenção e tratamento”. Universidade Aberta/IHMT/Fundação Calouste Gulbenkian. Coordination: Miguel Viveiros | Jorge Atouguia. (Outros) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2006/12 - 2007/02 Advanced training in molecular biology for the diagnosis of tuberculosis - Laboratory technicians from Laboratório de Micobactérias, Hospital Pulido Valente, Lisboa. Coordination: Miguel Viveiros. (Outros) Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal

Mentoring / Tutoring

Topic Student name
2014 - 2015 Research grantee in the FCT funded project “Type-II NADH-menaquinone oxidoreductase (NDH-2) and the respiratory chain of M. tuberculosis. Laura Fernandes
2013 - 2014 PhD thesis in Cellular and Molecular Biology. “Relação entre o mecanismo de efluxo e a resistência aos antimicobacterianos em isolados clínicos de Mycobacterium tuberculosis. Universidade Federal do Rio Grande do Sul – UFRGS. Tatiane Silveira
2012 - 2013 Contribuição dos sistemas de efluxo na resistência à claritromicina no complexo Mycobacterium avium. Vânia Silva
2012 - 2013 MSc Thesis in Medical Microbiology (UNL). “Novas estratégias no combate à tuberculose: a NADH-menaquinona desidrogenase do tipo II de Mycobacterium tuberculosis". Carlos Serra
2010 - 2011 MSc Thesis in Biomedical Sciences (IHMT/UNL). “Resistência mediada por efluxo em Escherichia coli: desenvolvimento de novas estratégias terapêuticas”. Antónia Pinto
2009 - 2010 MSc Thesis in Biomedical Sciences (IHMT/UNL). “Aplicação de métodos moleculares na identificação rápida de M/XDR-TB”. Samuel Francisco
2008 - 2009 MSc Thesis in Biomedical Sciences (IHMT/UNL). “Identificação molecular de micobactérias”. Pedro Cerca
Distinctions

Award

2020 Prize Pfizer/NOVAHealth para Melhor Poster na III Conferência Internacional NOVAhealth Chronic Disease and Infection – Infection, Cancer and Global Health
2018 Prize Gilead Sciences
Gilead Sciences Lda, Portugal
2017 Best Panel Communication - MICRO2017
Sociedade Portuguesa de Microbiologia, Portugal
2015 Prize Professor Doutor Manuel Pinto - Best PhD 2014 in Biomedical Sciences, speciality Microbiology
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal

Other distinction

2013 ESM travel grant and free registation for participation on the 34th Annual Congress of the European Society of Mycobacteriology
European Society of Mycobacteriology, Germany
2011 Free registration on 21st European Congress of Clinical Microbiology and Infectious Diseases
European Society of Clinical Microbiology and Infectious Diseases, Switzerland