Diana Machado is an Assistant Researcher at Unidade de Microbiologia Médica, Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL). She has a graduation in Biology (Universidade Lusófona de Humanidades e Tecnologias de Lisboa), a Master in Medical Microbiology (UNL) and a PhD in Biomedical Sciences, specialty Microbiology (IHMT/UNL). The scientific activity developed extents across the field of medical microbiology being transversal to antimicrobial resistance, epidemiology, and laboratory diagnosis of infectious diseases and aims to study the bacterial mechanisms underlying the emergence and evolution of drug resistance with epidemiological and clinical relevance aiming to use this information to develop medicines and technologies to combat bacterial infections. She is author/co-author of 64 scientific studies in peer-review national and international journals in myco(bacteriology) on drug resistance, mechanism of action of drugs, molecular epidemiology, and TB diagnosis, and more than 120 communications in congress and other scientific meetings (oral and poster). She has participated in 12 R&D projects as a team member (4 ongoing) and 2 as co-PI (1 ongoing); supervised 1 PhD and co-supervised 3 PhD students; supervised 9 MSc and co-supervised 8 MSc students; supervised 5 graduate students and 6 research internships. Has been participating in advanced training programs in mycobacteriology, and curricular units in NOVAs MSc and PhD programs contributing with lectures, laboratory teaching and evaluation. She is an Editorial Board Member of Journals BMC Infectious Diseases, Annals of Clinical Microbiology and Antimicrobials, Journal of Global Antimicrobial Resistance, and Fronteirs; is an ad-doc reviewer for several scientific journals (publons.com/a/1176897/). The primary research activities rely on the study of drug resistance in active and latent tuberculosis, understanding drug resistance mechanisms, exploring new antituberculosis drugs and targets, and new mechanisms for killing M. tuberculosis. Her studies also involve the evaluation of molecular systems for the detection of M. tuberculosis, screening of mutations that confer resistance to antituberculosis drugs and the direct detection of M. tuberculosis from clinical samples, evolutionary mechanisms, molecular epidemiology, comparative genomics of clinical isolates circulating in Portugal. Study of resistance mechanisms and molecular identification of nontuberculous mycobacteria. She has been involved in the laboratory diagnosis of TB and early molecular detection of M/XDR TB in Lisbon since 2006. The work done had and still has a positive impact on the decrease of TB incidence and allowed the control of M/XDRTB in Portugal allowing to establish protocols and multicenter studies in this field with e.g., Becton & Dickinson, EUCAST, ESGMYC, and TB Alliance, and pharmaceuticals like Janssen, Otsuka, and Tecnimede. Has participated in the validation of the SIRE KIT Nitratase for M. tuberculosis DST with the Brazilian TB lab network. She is also involved in the development of TB nanodiagnostics in collaboration with the Nanotheranostics Group FCT/UNL. In between, she is also studying the mechanisms of antimicrobial resistance and virulence of the Gram-negative bacterial species Acinetobacter baumannii, Escherichia coli, and Helicobacter pylori. The most recent projects include the study of the contribution of oxidative stress to persister formation and survival using Helicobacter pylori and the role of efflux inhibitors on viral infections, namely TB/HIV co-infection, focusing on host-pathogen interaction and inhibition of cell energy.