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Josué Leandro de Oliveira e Carvalho. Completed the Doutoramento in Biomedicina in 2020/12/10 by Universidade da Beira Interior Faculdade de Ciências da Saúde, Mestrado in Biotecnologia in 2015/07/13 by Universidade da Beira Interior Faculdade de Ciências and Licenciatura in Biotecnology in 2013/07 by Universidade da Beira Interior Faculdade de Ciências. Is R&D Scientist in ALS Life Sciences Portugal, S.A. and General Manager in Invitek Diagnostics. Published 28 articles in journals. Organized 1 event(s). Participated in 1 event(s). Co-supervised 1 PhD thesis(es). Supervised 4 MSc dissertation(s) e co-supervised 2. Supervised 4 work(s) of course completion of LSc/BSc e co-supervised 1. Has received 11 awards and/or honors. Participates and/or participated as Principal investigator in 1 project(s) and Researcher in 8 project(s). Works in the area(s) of Medical and Health Sciences with emphasis on Medical Biotechnology with emphasis on Health-Related Biotechnology and Engineering and Technology with emphasis on Industrial Biotechnology with emphasis on Industrial Biotechnology. In their professional activities interacted with 203 collaborator(s) co-authorship of scientific papers. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: G-quadruplex; Medicinal Chemistry; Biophysics; Nuclear Magnetic Resonance; Cell studies; Cancer research; Viral research; Nanotechnology; Drug delivery; Human Papillomavirus; solid-phase synthesis; oligonucleotides; PCR kits; LAMP kits; oligonucleotide production; product development; therapeutic oligonucleotides; hormones; cellular signalling; health biotechnology; biomolecular sciences; treatment; vaginal infections; .
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Identification

Personal identification

Full name
Josué Leandro de Oliveira e Carvalho
Date of birth
1989/04/02
Gender
Male

Citation names

  • Carvalho, Josué
  • Carvalho, J.
  • J. Carvalho

Author identifiers

Ciência ID
6E1B-8065-97D1
ORCID iD
0000-0003-3035-4881
Google Scholar ID
g_jJsRYAAAAJ
Scopus Author Id
56946235700

Email addresses

  • josue.carvalho@alsglobal.com (Professional)

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Engineering and Technology - Industrial Biotechnology - Industrial Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Proficiency (C2) Proficiency (C2) Advanced (C1) Advanced (C1)
French Beginner (A1) Elementary (A2) Beginner (A1) Elementary (A2)
Spanish; Castilian Advanced (C1) Advanced (C1) Upper intermediate (B2) Advanced (C1)
Education
Degree Classification
2022/11/14 - 2022/12/13
Concluded
 Formação professional em NP 4457:2021 - Gestão da Inovação (Outros)
ALS Controlvet - Segurança Alimentar SA, Portugal
2021/01/19 - 2021/01/19
Concluded
 Formação Profissional em Requisitos gerais para a competência de laboratórios de ensaio e calibração - ISO 17025 (Outros)
ALS Controlvet - Segurança Alimentar SA, Portugal
2020/12/15 - 2020/12/16
Concluded
 Formação Profissional de Dispositivos Médicos IVD - ISO 13485 (Outros)
ALS Controlvet - Segurança Alimentar SA, Portugal
2015 - 2020/12/10
Concluded
 Biomedicina (Doutoramento)
Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
"G-quadruplex aptamers for cervical cancer therapy" (THESIS/DISSERTATION)
 Approved with Distinction
2019/09/16 - 2019/09/20
Concluded
 Course in Laboratory Animal Science - FELASA Cat. B (Outros)
Universidade de Lisboa Faculdade de Farmácia, Portugal

Sociedade Portuguesa de Ciências em Animais de Laboratório, Portugal
17 (out of 20)
2013/01/09 - 2015/07/13
Concluded
 Biotecnologia (Mestrado)
Major in Molecular Biology
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Biosynthesis of a G-Quadruplex—forming sequence and its stabilization by ligands" (THESIS/DISSERTATION)
 19 (out of 20)
2010/09/01 - 2013/07
Concluded
 Biotecnology (Licenciatura)
Universidade da Beira Interior Faculdade de Ciências, Portugal
"Inibidores de Quorum Sensing: Estudo da actividade inibitória do TEP e da cafeína em Staphylococcus aureus" (THESIS/DISSERTATION)
 16 (out of 20)
Affiliation

Science

Category
Host institution 		Employer
2019/05/13 - 2019/05/17 Visiting Researcher (Research) Centro de Ciências e Tecnologias Nucleares, Portugal
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
2018/08 - 2018/08 Visiting Researcher (Research) Penn State College of Medicine Department of Microbiology and Immunology, United States
2016/02/01 - 2016/04/01 Research Trainee (Research) Institut Européen de Chimie et Biologie, France
2014/09/01 - 2015/07/31 Researcher (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2014/07/07 - 2014/08/29 Research Trainee (Research) Hochschule Emden Leer, Germany
2013/07/15 - 2013/08/15 Research Trainee (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2012/07/15 - 2012/08/30 Research Trainee (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Others

Category
Host institution 		Employer
2025/04/01 - Current General Manager Invitek Diagnostics, Portugal
2020/01/09 - Current R&D Scientist ALS Life Sciences Portugal, S.A., Portugal
2017/02/01 - 2020/09/01 PhD Fellowship Fundação para a Ciência e a Tecnologia, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2020/04/15 - 2020/06/01 Volunteer at COVID-19 Diagnostic Testing Lab Centro Hospitalar Cova da Beira EPE, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2015/11/02 - 2016/08/31 Doctoral Incentive Fellowship (ICI-CICS-BST-UBI) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Projects

Grant

Designation Funders
2023/01/01 - Current SMARTgNOSTICS - Global Testing & Diagnostics Solutions
C644915155-00000024
Principal investigator
ALS Life Sciences Portugal, S.A., Portugal
 European Union
Ongoing
2022/01/17 - 2023/06/30 NAMShield - Controlo de Salmonella spp., na indústria avícola, aplicando aptâmeros de mímicos de ácidos nucleicos nanoensapsulados
POCI-01- 0247-FEDER-069756
Principal investigator
ALS Life Sciences Portugal, S.A., Portugal
Concluded
2018 - 2021 Multifunctional Nanoseeds for Chemoradiotherapy of Glioblastoma
PTDC/MED-QUI/29649/2017
Researcher
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2020/05 - 2020/08 Track and Trace COVID-19
149_596826219
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019 - 2020 KRAS G-quadruplexes as an innovative theragnostic strategy for cervical cancer
5079
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Institut Européen de Chimie et Biologie, France
Fundação para a Ciência e a Tecnologia
Ongoing
2018 - 2020 BIOmedical DEVICE development based on pre-miRNA G-quadruplex
MIT-EXPL/BIO/0008/2017
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Massachusetts Institute of Technology Computer Science and Artificial Intelligence Laboratory, United States
Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2020 DRug dElivery nAnosysteM for HPV infection therapy
UTAP-EXPL/NTec/0015/2017
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

University of Texas at Austin, United States
Fundação para a Ciência e a Tecnologia
Ongoing
2018 - 2019 Development of drug delivery nanocarrier for HPV infection
45/2018
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Penn State College of Medicine Department of Microbiology and Immunology, United States
Fundação Luso-Americana
Concluded
2017 - 2019 G-quadruplex aptamers for cervical cancer therapy
TC-15/17
Researcher
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Institut Européen de Chimie et Biologie, France
Ongoing

Contract

Designation Funders
2019/01/01 - 2019/12/31 Health Sciences Research Centre
UID/Multi/00709/2019
Universidade da Beira Interior, Portugal

Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Conference abstract
  1. Carina Almeida; Faria, Márcia; Carvalho, Josué; Pinho, Eva. Corresponding author: Pinho, Eva. "Nanosystems to successfully delivery aptamers: A disruptive strategy prevent Salmonellosis on poultry industry". Paper presented in 2023 IAFP's European symposium, Aberdeen, Scotland, 2023.
Conference paper
  1. Miranda, André; Cruz, Carla; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Jardim, Andreia; Caneira, Catarina; et al. "Microfluidic assay based on G-quadruplex aptamer for nucleolin detection in prostate cancer". Paper presented in The 1st International Electronic Conference on Biosensors, 2020.
    10.3390/iecb2020-07064
  2. Gilmar F. Salgado; Julien Marquevielle; Carvalho, Josué; Carla Cruz; Coralie Robert; Luigi Xodo; Jean-Louis Mergny. "Understanding the role of proto-oncogene Kras G-quadruplexes: Structural and ligand binding characterization.". Paper presented in JOINT 12th EBSA congress and 10th ICBP – IUPAP congress, 2019.
    10.1007/s00249-019-01373-4
Conference poster
  1. Faria, Márcia; Carina F. Almeida; Filipe B. Sousa; Daniela Silva; Pinho, Eva; Carvalho, Josué. "Targeting Sipa Protein with NAM-Aptamers as an Innovative Approach to Control Salmonellosis in the Poultry Industry". Paper presented in 2023 IAFP's European symposium, 2023.
  2. Carina Almeida; Faria, Márcia; Nogueira, Teresa; Carvalho, Josué; Pinho, Eva. "Controlling Salmonellosis on poultry industry through the use of aptamers: Identification of protein targets". Paper presented in IAFP's European symposium, 2023.
  3. Faria, Márcia; Carina F. Almeida; Filipe B. Sousa; Daniela Silva; Pinho, Eva; Carvalho, Josué. "NAM-Aptamers based approch to control Salmonellosis in the poulty industry". Paper presented in Dare2Change, 2023.
  4. Joana Figueiredo; André Miranda; Jéssica Lopes-Nunes; Carvalho, Josué; Daniela Alexandre; Salete Valente; Carla Cruz. "Estudo da expressão da Nucleolina no cancro de pulmão de não-pequenas células". Paper presented in 3º ENJIO - ENCONTRO NACIONAL DE JOVENS INVESTIGADORES EM ONCOLOGIA, 2021.
  5. Figueiredo, Joana; Lopes-Nunes, Jéssica; Miranda, André; Carvalho, Josué; Alexandre, Daniela; Valente, Salete; Mergny, Jean-Louis; Cruz, Carla. "Estudo da expressão da nucleolina no cancro do pulmão de não-pequenas células". Paper presented in I SEMANA DA INVESTIGAÇÃO DO CHUCB, 2020.
  6. Figueiredo, Joana; Lopes-Nunes, Jéssica; Miranda, André; Carvalho, Josué; Alexandre, Daniela; Valente, Salete; Mergny, Jean-Louis; Cruz, Carla. "Estudo da expressão da nucleolina no cancro do pulmão de não-pequenas células (Co-author; presented by: Joana Figueiredo)". Paper presented in I SEMANA DA INVESTIGAÇÃO DO CHUCB, 2020.
  7. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Vaz, Virgilio; Pereira, Bruno J.; Chu, Virginia; Conde, João P.; Cruz, Carla. "Nucleolina: um biomarcador clinicamente relevante em doentes com cancro da próstata?". Paper presented in I Semana da Investigação do Centro Hospitalar Universitário Cova da Beira, 2020.
  8. Gomes-Maocha, Izamara; Carvalho, Josué; Lopes-Nunes, Jéssica; Tomás, Mariana; Agonia, Ana Sofia; Palmeira-de-Oliveira, R.; Palmeira-de-Oliveira, A.; Martinez-de-Oliveira, José; Cruz, Carla. "Development of drug-loaded gel formulations for intravaginal administration of anti-HPV drugs". Paper presented in XV ANNUAL CICS-UBI SYMPOSIUM 2020, 2020.
  9. Carvalho, J.; Lopes-Nunes, Jéssica; Milici, Janice; Meyers, Craig; Mergny, Jean-Louis; Salgado, Gilmar F.; Cruz, Carla. "Targeting Human Papillomavirus Promoter Regions as a Potential Antiviral Strategy". Paper presented in IPVC2020 - 33rd International Papillomavirus Conference, 2020.
  10. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Jardim, Andreia; Caneira, Catarina R. F.; Vaz, Virgilio; et al. "Detection of Nucleolin Using a Microfluidic Assay Based on G Quadruplex Aptamer". Paper presented in Encontro Ciência 2020, 2020.
  11. Jardim, Andreia; Huck, Michael; Catarina R. F. Caneira; Carvalho, Josué; Santos, Tiago; Chu, Virginia; Cruz, Carla; Conde, João P.. "Detection of the potential cancer biomarker nucleolin in a microfluidic system". Paper presented in III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer, 2019.
  12. Miranda, André; Santos, Tiago; Carvalho, Josué; Chu, Virginia; Conde, João P.; Cruz, Carla. "Molecular Beacon Derived from AS1411 to Nucleolin Detection". Paper presented in III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer, 2019.
  13. Miranda, André; Santos, Tiago; Carvalho, Josué; Chu, Virginia; Conde, João P.; Cruz, Carla. "AS1411 Derived Molecular Beacon to Nucleolin Detection". Paper presented in 2nd Portuguese Symposium on Research and Innovations in Urology, 2019.
  14. Figueiredo, Joana; Lopes-Nunes, Jéssica; Carvalho, Josué; Mergny, Jean-Louis; Cruz, Carla. "Derivados de AS1411 como sistemas de entrega direcionada de ligandos de G-quadruplex para células do cancro cervical". Paper presented in 1º ENCONTRO NACIONAL DE JOVENS INVESTIGADORES EM ONCOLOGIA 2019, 2019.
  15. Lopes-Nunes, Jéssica; Carvalho, Josué; Ferreira L, Catarina; Palmeira-de-Oliveira, Rita; Palmeira-de-Oliveira, Ana; Moutinho, José; Martinez-de-Oliveira, José; Cruz, Carla. "Formulation of a nanosystem-loaded topical gel for cervical cancer therapy". Paper presented in UT Austin Portugal Program 2019 Annual Conference “Create Knowledge, Foster Change: Towards a New Decade of Joint Achievements”, 2019.
  16. Cabral Campello, Maria Paula; Lopes-Nunes, Jéssica; Carvalho, Josué; Gano, Lurdes; Oliveira, Maria Cristina; Peitinho, David; Silva, Francisco; Paulo, António; Cruz, Carla. "Evaluation of the cytotoxic activity of the AS1411-AO-nanosystem for HPV cancer therapy (Co-author; presented by: Maria Paula Cabral Campello)". Paper presented in Nanotechnology in Cancer: Engineering for Oncology, 2019.
  17. Cabral Campello, Maria Paula; Lopes-Nunes, Jéssica; Carvalho, Josué; Gano, Lurdes; Oliveira, Maria Cristina; Peitinho, David; Silva, Francisco; Paulo, António; Cruz, Carla. "Evaluation of the cytotoxic activity of the AS1411-AO-nanosystem for HPV cancer therapy". Paper presented in Nanotechnology in Cancer: Engineering for Oncology, 2019.
  18. Alexandre, Daniela J.; Santos, Tiago; Carvalho, Josué; Sousa, Adriana; Pereira, Bruno J.; Sousa, Fani; Chu, Virginia; Conde, João P.; Cruz, Carla. "Nucleolin detection for diagnosis of prostate cancer". Paper presented in XIV Annual CICS-UBI Symposium, 2019.
  19. Miranda, André; Santos, Tiago; Carvalho, Josué; Queiroz, João A.; Chu, Virginia; Conde, João P.; Cruz, Carla. "G-quadruplex Aptamer Beacons for the Detection of Nucleolin". Paper presented in XIV Annual CICS-UBI Symposium, 2019.
  20. Lopes-Nunes, Jéssica; Carvalho, Josué; Cruz, Carla. "G-quadruplex aptamers-gold nanoparticles as drug delivery systems for cervical cancer". Paper presented in Nano Today Conference, 2019.
  21. J. Lopes Nunes; J. Carvalho; C. L. Ferreira; C.Cruz. "G-quadruplex aptamer-gold nanoparticles as drug delivery systems for cervical cancer". Paper presented in 6th Nano Today Conference, 2019.
  22. Carla Cruz; Carvalho, Josué; Gilmar F. Salgado; Jean-Louis Mergny; Craig Meyers. "Development of G-quadruplex mediated HPV antiviral drugs". Paper presented in EUROGIN 2018, 2018.
  23. Carvalho, Josué; J.-L. Mergny; C. Meyers; G. F. Salgado; J. A. Queiroz; C. Cruz. "Screening of G-quadruplex Ligands for Human Papillomaviruses". Paper presented in Biology of non-canonical nucleic acids: from humans to pathogens - BIONIC 2018, 2018.
  24. Carrilho, Rui; Santos, Tiago; Carvalho, Josué; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Study of the interaction between a RNA G-quadruplex aptamer and ligands". Paper presented in XIII Annual CICS-UBI symposium 2018, 2018.
  25. Carvalho, Josué; J.-L. Mergny; G. F. Salgado; J. A. Queiroz; C. Cruz. "Aptamer-based targeted delivery of G-quadruplex ligands in HeLa cervical cancer cells.". Paper presented in XII Spanish-Portuguese Conference on Controlled Drug Delivery, 2018.
  26. Carvalho, Josué; J.-L. Mergny; G. F. Salgado; J. A. Queiroz; C. Cruz. "Targeting Human Papilloma Virus G-rich Regions by an Aptamer-based G-quadruplex Ligands Delivery System.". Paper presented in Advances in Noncanonical Nucleic Acids ANNA 2017, 2017.
  27. Carvalho, Josué; S. Alves; M. M. C. A. Castro; C. F. G. C. Geraldes; C. P. Fonseca; J. A. Queiroz; C. Cruz. "Development of a NMR bioreactor for the evaluation of 5-Fluorouracil cytotoxicity in living human cells.". Paper presented in XII Annual CICS-UBI Symposium, 2017.
  28. Maria Paula Cabral Campello; Carvalho, Josué; Pererira, E; Marquevielle, J.; Mergny, JL; António Manuel da Rocha Paulo; Gilmar Salgado; Queiroz, JA.; Carla Patrícia Alves Freire Madeira Cruz. "Fluorescent Acridine Orange ligands derivatives bind and stabilize KRAS-22RT G-quadruplex". Paper presented in ”, II International Congress in Health Sciences Research, Towards Innovation and Entrepreneurship, Trends in Biotechnology for Biomedical Applications, Universidade da Beira Interior, 2017.
  29. Carvalho, Josué; E. Pereira; J. Marquevielle; M. P. C. Campello; J.-L. Mergny; A. Paulo; G. F. Salgado; J. A. Queiroz; C. Cruz. "KRAS-22RT G-quadruplex stabilization by Acridine Orange derivatives.". Paper presented in 6th International Meeting on Quadruplex Nucleic Acids, 2017.
  30. J. Marquevielle; Carvalho, Josué; C. Robert; M. Zuffo; A. Paulo; C. Cruz; F. Thomas; et al. "Structure and ligand binding studies of a G-Quadruplex found in the KRAS proto-oncogene promoter sequence.". Paper presented in 6th International Meeting on Quadruplex Nucleic Acids, 2017.
  31. Carvalho, Josué; Pereira, E.; Campelo, M. P. C.; Queiroz, João A.; Paulo, A.; Gilmar F. Salgado; Cruz, Carla. "Intracellular co-localization studies of G-quadruplex ligands by confocal fluorescence microscopy.". Paper presented in Advances in Cell Engineering, Imaging and Screening, 2016.
  32. Carvalho, Josué; S. Alves; C. P. Fonseca; C. Cruz. "Cytotoxicity evaluation of a calix[4]arene derivative in living human cells using 31P NMR spectroscopy.". Paper presented in First All Hands Meeting of iNEXT, 2016.
  33. Carvalho, Josué; J.-L. Mergny; J. A. Queiroz; G. F. Salgado; C. Cruz. "Study of the interaction between a novel indole-based compound and the biologically relevant c-MYC G-quadruplex.". Paper presented in 1st joint French-Portuguese NMR and XXVII GERM Conference, 2016.
  34. J. Ferreira; T. Santos; Carvalho, Josué; P. Pereira; J. A. Queiroz; F. Sousa; C. Cruz. "Purification of plasmid DNA using a chromatographic suport derived from G-quadruplex ligands.". Paper presented in XVI Latin-American Congress on Chromatography and 9th National Meeting on Chromatography, 2016.
  35. Ferreira, João; Santos, Tiago; Carvalho, Josué; Pereira, Patricia; Queiroz, João A.; Sousa, Fani; Cruz, Carla. "Purification of plasmid DNA using a chromatographic support derived from G-quadruplex ligands". Paper presented in XVI Latin-American Congress on Chromatography and 9th National Meeting on Chromatography, 2016.
  36. J. Silva; Carvalho, Josué; E. J. Cabrita; C. Cruz. "G-quadruplex production and stabilization.". Paper presented in 5th International Meeting on Quadruplex Nucleic Acids, 2015.
  37. Carvalho, Josué; J. Ferreira; P. Pereira; E. Coutinho; F. Sousa; E. J. Cabrita; J. A. Queiroz; C. Cruz. "Synthesis and characterization of novel naphthalene and quinoline-based G-quadruplex ligands for cancer therapy.". Paper presented in I International Congress in Health Sciences Research, 2015.
  38. Carvalho, Josué; J. Ferreira; E. Coutinho; Eurico J. Cabrita. "G-quadruplex-forming sequence production and stabilization.". Paper presented in III Jornadas de Química e Bioquímica, 2015.
Journal article
  1. Almeida, Carina F.; Faria, Márcia; Carvalho, Josué; Pinho, Eva. "Contribution of nanotechnology to greater efficiency in animal nutrition and production". Journal of Animal Physiology and Animal Nutrition (2024): http://dx.doi.org/10.1111/jpn.13973.
    Published • 10.1111/jpn.13973
  2. Maocha, Izamara Gomes; Carvalho, Josué; Lopes-Nunes, Jéssica; Rosado, Tiago; Gallardo, Eugénia; Tomás, Mariana; Palmeira-de-Oliveira, Ana; et al. "Drug formulations for localized treatment of Human Papillomavirus-induced lesions". Journal of Pharmaceutical Sciences (2022): http://dx.doi.org/10.1016/j.xphs.2022.02.004.
    Published • 10.1016/j.xphs.2022.02.004
  3. Silva, Francisco; D’Onofrio, Alice; Mendes, Carolina; Pinto, Catarina; Marques, Ana; Campello, Maria Paula Cabral; Oliveira, Maria Cristina; et al. "Radiolabeled Gold Nanoseeds Decorated with Substance P Peptides: Synthesis, Characterization and In Vitro Evaluation in Glioblastoma Cellular Models". International Journal of Molecular Sciences 23 2 (2022): 617. http://dx.doi.org/10.3390/ijms23020617.
    10.3390/ijms23020617
  4. Carvalho, Josué; Lopes-Nunes, Jéssica; Figueiredo, Joana; Santos, Tiago; Miranda, André; Riscado, Micaela; Sousa, Fani; et al. Corresponding author: Cruz, Carla. "Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection". Sensors 21 21 (2021): 7015. http://dx.doi.org/10.3390/s21217015.
    10.3390/s21217015
  5. Carvalho, Josué; Lopes-Nunes, Jéssica; Vialet, Brune; Rosado, Tiago; Gallardo, Eugenia; Vale, João; Eloy, Catarina; et al. "Nanoaggregate-forming lipid-conjugated AS1411 aptamer as a promising tumor-targeted delivery system of anticancer agents in vitro". Nanomedicine: Nanotechnology, Biology and Medicine 36 (2021): 102429. http://dx.doi.org/10.1016/j.nano.2021.102429.
    Published • 10.1016/j.nano.2021.102429
  6. Cadoni, Enrico; Magalhães, Pedro R.; Emídio, Rita M.; Mendes, Eduarda; Vítor, Jorge; Carvalho, Josué; Cruz, Carla; Victor, Bruno L.; Paulo, Alexandra. "New (Iso)quinolinyl-pyridine-2,6-dicarboxamide G-Quadruplex Stabilizers. A Structure-Activity Relationship Study". Pharmaceuticals 14 7 (2021): 669. http://dx.doi.org/10.3390/ph14070669.
    10.3390/ph14070669
  7. Palma, Elisa; Carvalho, Josué; Cruz, Carla; Paulo, António. "Metal-Based G-Quadruplex Binders for Cancer Theranostics". Pharmaceuticals 14 7 (2021): 605. http://dx.doi.org/10.3390/ph14070605.
    10.3390/ph14070605
  8. Miranda, André; Santos, Tiago; Carvalho, Josué; Alexandre, Daniela; Jardim, Andreia; Caneira, CatarinaR.F.; Vaz, Vírgilio; et al. "Aptamer-based approaches to detect nucleolin in prostate cancer". Talanta 226 (2021): 122037. http://dx.doi.org/10.1016/j.talanta.2020.122037.
    10.1016/j.talanta.2020.122037
  9. Figueiredo, Joana; Miranda, André; Lopes-Nunes, Jéssica; Carvalho, Josué; Alexandre, Daniela; Valente, Salete; Mergny, Jean-Louis; Cruz, Carla. "Targeting Nucleolin By RNA G-Quadruplex-Forming Motif". Biochemical Pharmacology (2021): 114418. http://dx.doi.org/10.1016/j.bcp.2021.114418.
    10.1016/j.bcp.2021.114418
  10. Carvalho, J.; Jéssica Lopes-Nunes; Maria PC Campello; António Paulo; Janice Milici; Craig Meyers; Jean-Louis Mergny; et al. "Human Papillomavirus G-Rich Regions as Potential Antiviral Drug Targets". Nucleic Acid Therapeutics (2020):
    In press • dx.doi.org/10.1089/nat.2020.0869
  11. Cruz, Carla; Pedro, Augusto Q.; Carvalho, Josué; Santos, Tiago; Talhada, Daniela; Paiva, Artur; Queiroz, João A.; et al. "Nucleolin as a potential biomarker for canine malignant neoplasia". Research in Veterinary Science (2020): http://dx.doi.org/10.1016/j.rvsc.2020.10.004.
    In press • 10.1016/j.rvsc.2020.10.004
  12. Carvalho, Josué; Mergny, Jean-Louis; Salgado, Gilmar F.; Queiroz, João A.; Cruz, Carla. "G-quadruplex, Friend or Foe: The Role of the G-quartet in Anticancer Strategies". Trends in Molecular Medicine (2020): http://dx.doi.org/10.1016/j.molmed.2020.05.002.
    10.1016/j.molmed.2020.05.002
  13. Lopes-Nunes, Jéssica; Carvalho, Josué; Figueiredo, Joana; Ramos, Catarina I.V.; Lourenço, Leandro M.O.; Tomé, João P.C.; G.P.M.S. Neves, Maria; et al. "Phthalocyanines for G-quadruplex aptamers binding". Bioorganic Chemistry 100 (2020): 103920. http://dx.doi.org/10.1016/j.bioorg.2020.103920.
    10.1016/j.bioorg.2020.103920
  14. Carvalho, Josué; Cruz, Carla. "Forster resonance energy transfer for studying nucleic acids denaturation: A chemical and biological sciences laboratory experiment". Biochemistry and Molecular Biology Education (2020): http://dx.doi.org/10.1002/bmb.21353.
    10.1002/bmb.21353
  15. Eddahmi, Mohammed; Moura, Nuno M. M.; Bouissane, Latifa; Faustino, Maria A. F.; Cavaleiro, José A. S.; Paz, Filipe A. A.; Mendes, Ricardo F.; et al. "Synthesis and Biological Evaluation of New Functionalized Nitroindazolylacetonitrile Derivatives". ChemistrySelect 4 48 (2019): 14335-14342. http://dx.doi.org/10.1002/slct.201904344.
    10.1002/slct.201904344
  16. Figueiredo, Joana; Lopes-Nunes, Jéssica; Carvalho, Josué; Antunes, Francisca; Ribeiro, Márcia; Campello, Maria Paula Cabral; Paulo, António; et al. "AS1411 derivatives as carriers of G-quadruplex ligands for cervical cancer cells". International Journal of Pharmaceutics (2019): http://dx.doi.org/10.1016/j.ijpharm.2019.118511.
    10.1016/j.ijpharm.2019.118511
  17. Carvalho, Josué; Santos, Tiago; Carrilho, Rui; Sousa, Fani; Salgado, Gilmar F.; Queiroz, João António; Cruz, Carla. "Ligand Screening to pre-miRNA 149 G-quadruplex investigated by Molecular Dynamics". Journal of Biomolecular Structure and Dynamics (2019): 1-15. http://dx.doi.org/10.1080/07391102.2019.1632743.
    10.1080/07391102.2019.1632743
  18. Carvalho, Josué; Paiva, Artur; Cabral Campello, Maria Paula; Paulo, António; Mergny, Jean-Louis; Salgado, Gilmar F.; Queiroz, João A.; Cruz, Carla. "Aptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells". Scientific Reports 9 1 (2019): http://dx.doi.org/10.1038/s41598-019-44388-9.
    Open access • 10.1038/s41598-019-44388-9
  19. Catarina I. V. Ramos; Susana P. Almeida; Leandro M. O. Lourenço; Patrícia M. R. Pereira; Rosa Fernandes; M. Amparo F. Faustino; João P. C. Tomé; et al. "Multicharged Phthalocyanines as Selective Ligands for G-Quadruplex DNA Structures". Molecules 24 4 (2019): 733.
    Open access • 10.3390/molecules24040733
  20. Carvalho, Josué; Nunes, Jéssica Lopes; Lopes, Ana Catarina; Cabral Campello, Maria Paula; Paulo, António Rocha; Queiroz, João António; Cruz, Carla. "Aptamer-guided acridine derivatives for cervical cancer". Organic & Biomolecular Chemistry (2019): http://dx.doi.org/10.1039/c9ob00318e.
    10.1039/c9ob00318e
  21. Josué Carvalho; Alves, Sara; Castro, M. Margarida C.A.; Geraldes, Carlos F.G.C.; Queiroz, João A.; Fonseca, Carla P.; Cruz, Carla. "Development of a bioreactor system for cytotoxic evaluation of pharmacological compounds in living cells using NMR spectroscopy". Journal of Pharmacological and Toxicological Methods 95 (2019): 70-78. http://dx.doi.org/10.1016/j.vascn.2018.11.004.
    10.1016/j.vascn.2018.11.004
  22. Josué Carvalho; Telma Quintela; Nassima M Gueddouda; Carla Cruz; Anne Bourdoncle; Jean-Louis Mergny; Gilmar Salgado; João António Queiroz. "Phenanthroline polyazamacrocycles as G-quadruplex DNA binders". Organic & Biomolecular Chemistry (2018): https://doi.org/10.1039%2Fc8ob00247a.
    10.1039/c8ob00247a
  23. Carvalho, Josué; E. Pereira; J. Marquevielle; M. P. C. Campello; Jean-Louis Mergny; A. Paulo; G. F. Salgado; J. A. Queiroz; C. Cruz. "Fluorescent light-up acridine orange derivatives bind and stabilize KRAS-22RT G-quadruplex". Biochimie 144 (2017): 144-152. http://www.sciencedirect.com/science/article/pii/S0300908417302845.
    10.1016/j.biochi.2017.11.004
  24. Josué Carvalho; João A. Queiroz; Carla Cruz. "Circular Dichroism of G-Quadruplex: a Laboratory Experiment for the Study of Topology and Ligand Binding". Journal of Chemical Education 94 10 (2017): 1547-1551. https://doi.org/10.1021/acs.jchemed.7b00160.
    10.1021/acs.jchemed.7b00160
  25. Josué Carvalho; Pierre Nottelet; Jean-Louis Mergny; João A. Queiroz; Gilmar F. Salgado; Carla Cruz. "Study of the interaction between indole-based compounds and biologically relevant G-quadruplexes". Biochimie 135 (2017): 186-195. https://doi.org/10.1016/j.biochi.2017.02.005.
    10.1016/j.biochi.2017.02.005
  26. Carvalho, Josué; Tiago Santos; MC Corvo; Eurico J. Cabrita; J. A. Queiroz; C. Cruz. "L-tryptophan and dipeptide derivatives for supercoiled plasmid DNA purification". International Journal of Biological Macromolecules 87 (2016): 385-396. http://www.sciencedirect.com/science/article/pii/S0141813016302215.
    10.1016/j.ijbiomac.2016.02.079
  27. Carvalho, Josué; João Ferreira; P. Pereira; E. Coutinho; Aurore Guédin; Pierre Nottelet; G. F. Salgado; et al. "Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands". Tetrahedron 72 9 (2016): 1229-1237. http://www.sciencedirect.com/science/article/pii/S0040402016300229.
    10.1016/j.tet.2016.01.022
  28. Ferreira, Soraia; Carvalho, Josué; Valente, Joana F.A.; Corvo, Marta C.; Cabrita, Eurico J.; Sousa, Fani; Queiroz, João A.; Cruz, Carla. "Affinity analysis and application of dipeptides derived from l -tyrosine in plasmid purification". Journal of Chromatography B 1006 (2015): 47-58. http://dx.doi.org/10.1016/j.jchromb.2015.10.025.
    10.1016/j.jchromb.2015.10.025
Thesis / Dissertation
  1. Carvalho, J.. "G-quadruplex aptamers for cervical cancer therapy". PhD, Universidade da Beira Interior, 2020.
  2. Carvalho, Josué. "Biosynthesis of a G-Quadruplex—forming sequence and its stabilization by ligands". Master, Universidade da Beira Interior Faculdade de Ciências, 2015. http://hdl.handle.net/10400.6/6472.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/11/14 Human Papillomavirus G-Rich Regions as Potential Targets for Antiviral Drug Development III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship
(Portugal)
2019/07/05 Aptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells XIV Annual CICS-UBI Symposium
(Portugal)
2019/06/20 AS1411 aptamer-conjugated polymeric micelle for the delivery of antiviral and anticancer agents 6th Nano Today Conference
Elsevier (Lisbon, Portugal)
2018/07 Nucleolin-targeted G-quadruplex aptamer as a drug delivery system for cervical cancer cells XIII Annual CICS-UBI Symposium
(Portugal)
2018/03 Nucleolin-targeted G-quadruplex Aptamers as Drug Delivery Systems for Cervical Cancer VI Encontro Nacional de Estudantes de Química
(Portugal)
2017/05 Fluorescent Acridine Orange derivatives bind and stabilize KRAS-22RT G-quadruplex II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship
(Portugal)
2017/04 G-quadruplex: emerging therapeutic targets for anticancer drug development III Ciclo de Conferências em Biotecnologia Medicinal e Farmacêutica
(Portugal)
2017/02 G-quadruplex: a novel anticancer strategy World Cancer Day
(Portugal)
2017/01 Acridine Orange derivatives as G-quadruplex ligands V Ciclo de Conferências da Faculdade de Ciências
(Portugal)
2016/06 Targeting G-quadruplexes: Novel Indole-based Ligands XI Annual CICS-UBI Symposium
(Portugal)
2015/07 Biosynthesis of a G-Quadruplex—forming sequence and its stabilization by naphthalene-based ligands X Annual CICS-UBI Symposium
(Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2024/09/09 - Current Desenvolvimento e validação de um kit liofilizado para serotipagem e STEC para aplicações em segurança alimentar
Supervisor
 Bioquímica (Master)
Universidade da Beira Interior, Portugal
2024/09/09 - Current Desenvolvimento e validação de um método PCR em tempo real para deteção de Mycoplasma spp. no controlo de culturas celulares e vacinas
Supervisor
 Microbiologia (Master)
Universidade de Aveiro, Portugal
2024 - Current Control of salmonellosis, in the poultry industry, employing nanoencapsulated aptamers of nucleic acid mimics.
Co-supervisor
 Bioengenharia (PhD)
Universidade do Minho, Portugal
2022/08 - 2024/04 Implementação do Regulamento UE 2017/746, no âmbito do referencial normativo EN ISO 13485:2016
Co-supervisor
 Ciências Farmacêuticas (Master)
Universidade da Beira Interior, Portugal
2022/10/10 - 2023/12 Implementação e validação do método de deteção de DNA do alergénio de coco por real-time PCR
Supervisor
 Biologia Molecular e Celular (Master)
Universidade de Aveiro, Portugal
2022/08/23 - 2023/07/13 Desenvolvimento e implementação de um método de deteção molecular de clostridia produtores de neurotoxinas botulínicas tipo A, B, E e F por PCR de acordo com a norma ISO 17919
Supervisor
 Biotecnologia (Master)
Universidade da Beira Interior, Portugal

ALS Life Sciences Portugal, S.A., Portugal
2022/01 - 2022/07 Desenvolvimento de um aptamero radiomarcado anti-PDL1 para teranóstica do cancro do pulmão
Supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior, Portugal
2020/02 - 2021/03/10 Formulação de um sistema de administração uterino para controlar a replicação do vírus do Papiloma Humano
Co-supervisor
 Mestrado em Ciências Biomédicas (Master)
Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
2019/02 - 2019/06 Estudo do efeito do solvente na formulação de micelas funcionalizadas com aptameros
Co-supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior Faculdade de Ciências, Portugal
2017/02 - 2017/07 Conjugação de aptameros a agentes de escape endossomal para entrega de fármacos
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Faculdade de Ciências, Portugal
2017/02 - 2017/02 Optimização do aptamero AS411 com aumento da afinidade para células cancerígenas
Supervisor
 Biotecnologia (Degree)
Universidade da Beira Interior Faculdade de Ciências, Portugal
2016/02 - 2016/07 Aptameros de G-quadruplex radiomarcados para a deteção de células cancerígenas infetadas por VPH
Supervisor
 Bioquímica (Degree)
Universidade da Beira Interior Faculdade de Ciências, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2019/01/01 - 2019/11/30 III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer (2019/11/14 - 2019/11/16)
Congress (Member of the Organising Committee)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2019/04/06 - 2019/04/06 Participation as FCT funded PhD fellow in representation of the R&D unit CICS-UBI. PhD students presented their projects and papers, and answer the question "what is a PhD" in their field of study.
Exhibition
 Futurália 2019
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Committee member

Activity description
Role 		Institution / Organization
2018 - Current Doctoral Students Committee of the University of Beira Interior Health Sciences Research Center (CAD-CICS)
Member
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2020/04/15 - 2020/04/15 Estrutura e Função de Proteínas Bioquímica (Mestrado integrado) Universidade da Beira Interior, Portugal
2020/04/15 - 2020/04/15 Estrutura e Função de Proteínas Biotecnologia (Mestrado integrado) Universidade da Beira Interior, Portugal
2019/04/10 - 2019/04/10 Estrutura e Função de Proteínas Bioquímica (Mestrado integrado) Universidade da Beira Interior, Portugal
2018/04/18 - 2018/04/19 Estrutura e Função de Proteínas Biotecnologia (Mestrado integrado) Universidade da Beira Interior, Portugal
2018/04/18 - 2018/04/19 Estrutura e Função de Proteínas Bioquímica (Mestrado integrado) Universidade da Beira Interior, Portugal
2017/02/21 - 2017/06/06 Bioquímica II Biotecnologia (Licenciatura) Universidade da Beira Interior, Portugal
2016/02/10 - 2016/04/06 Bioquímica II Biotecnologia (Licenciatura) Universidade da Beira Interior, Portugal

Mentoring / Tutoring

Topic Student name
2019/07/01 - 2019/07/31 International Federation of Medical Students’ Associations (IFMSA) Exchange Program Vanja Hrešcak
2019/07/01 - 2019/07/31 International Pharmaceutical Students’ Federation (IPSF) Exchange Program Milica Vlatkovic
2019/07/01 - 2019/07/31 International Pharmaceutical Students’ Federation (IPSF) Exchange Program Emilija Krstic
2019/01/21 - 2019/04/30 Avaliação da expressão de nucleolina em tecidos com lesão pré-cancerosa induzida por HPV Maria Luísa Noites Sacramento
2019/01/21 - 2019/04/30 Identificação da nucleolina como um potencial biomarcador tumoral do cancro da próstata Nuno Alexandre França Rua
2017/07/03 - 2017/07/28 International Federation of Medical Students’ Associations (IFMSA) Exchange Program Maha El Barch
2017/07/10 - 2017/07/14 Ciência Viva no Laboratório Ricardo Pedrosa, Beatriz Coelho, Inês Veloso
2016/07/01 - 2016/07/31 International Federation of Medical Students’ Associations (IFMSA) Exchange Program Carmen Vizoso
2016/07/01 - 2016/07/31 International Federation of Medical Students’ Associations (IFMSA) Exchange Program Jin Luo

Other jury / evaluation

Activity description Institution / Organization
2020/06/30 - 2020/06/30 Jury of the final project entitled "MicroRNAs circulantes em biópsias líquidas para diagnóstico do cancro da próstata". Work presented by Joana Polido in the scope of "Projecto em Biotecnologia" course towards the obtaining of Biotecnologia Degree. Universidade da Beira Interior, Portugal
2019/06/25 - 2019/06/25 Jury of the final project entitled "Nanopartículas de ouro guiadas por aptameros de G-quadruplex para entrega de fármacos em células tumorais do colo do útero". Work presented by André Rico in the scope of "Projecto em Bioquímica" course towards the obtaining of Bioquímica Degree. Universidade da Beira Interior Faculdade de Ciências, Portugal
2017/07/27 - 2017/07/27 Jury of the final project entitled "Microagulhas poliméricas para entrega de fármacos a células cancerígenas". Work presented by Daniela Sofia Bento in the scope of "Projecto em Bioquímica" course towards the obtaining of Bioquímica Degree. Universidade da Beira Interior Faculdade de Ciências, Portugal
Distinctions

Award

2020 "Best E-poster communication award" in IPVC2020 - 33rd International Papillomavirus Conference
2020 "Best poster award" in 1st International Electronic Conference on Biosensors
2019 "Best oral communication award" in XIV Annual CICS-UBI Symposium
2017 "Best oral communication award" in II International Congress in Health Sciences Research
2017 "Best poster communication award" in XII Annual CICS-UBI Symposium
2017 SCIENT Business Idea Competition Winner
2016 "Best oral communication award" in XI Annual CICS-UBI Symposium
2015 Merit Scholarship
Universidade da Beira Interior, Portugal
2015 "Best poster communication award" in Jornadas de Química e Bioquímica 2015
2014 School Award for best course classification
Universidade da Beira Interior, Portugal
2013 Merit Scholarship
Universidade da Beira Interior, Portugal