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Margarida Espada is currently a member of MED ¿ (Mediterranean Institute for Agriculture, Environment and Development at University of Évora, Évora, Portugal). In 2012, she was awarded with a PhD grant funded by the Portuguese Government (FCT) (2013-2017) and finished her PhD in Biology at University of Évora in 2018. Her PhD thesis was a joint project between Portugal and Scotland (The James Hutton Institute), where she worked for more than 8 months, improving knowledge and skills (e.g.bioinformatics). Her main research interest is in molecular basis of plant-nematode interactions, particularly in the identification and functional characterisation of parasitism proteins (effectors) of plant-parasitic nematodes and understanding their roles, using genomic and transcriptomic approaches. M Espada has been author of 16 peer-reviewed scientific publications, 3 book sub-chapters and attended several national and international conferences presenting her work in oral and panel communications and was invited speaker in 2 seminars (ICAAM and Gent University) and participated in 2 international scientific networks. Supervised/co-supervised one MSc in an EU Master program and several undergraduate students. M Espada is the Principal Investigator of an ongoing project (2022) and participated in 7 scientific projects (scientific team and/or fellowships). M Espada has specialised in nematology, transcriptomics, molecular and cell biology and plant pathology. Recently was awarded with a Junior Researcher contract in 2020.
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Identification

Personal identification

Full name
Margarida Espada

Citation names

  • Espada, Margarida
  • Espada, M

Author identifiers

Ciência ID
671D-7737-92A4
ORCID iD
0000-0002-3826-711X
Scopus Author Id
54385053900

Email addresses

  • mespada@uevora.pt (Professional)

Addresses

  • MED - Mediterranean Institute for Agriculture, Environment and Development. Universidade de Évora, Pólo da Mitra, 7006-554, Évora, Évora, Portugal (Professional)

Websites

Knowledge fields

  • Agrarian Sciences - Agriculture, Forestry and Fisheries - Plant Breeding and Plant Protection
  • Natural sciences - Biological Sciences - Biology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Plant sciences
Education
Degree Classification
2018/03/22
Concluded
 PhD in Biology (European title) (Doutoramento)
Universidade de Évora, Portugal
"Functional characterization of putative effectors in pinewood nematode, Bursaphelenchus xylophilus." (THESIS/DISSERTATION)
 Aprovado com Distinção e Louvor
2010/11/30
Concluded
 Masters in Human Biology and Environment (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Envolvimento da via de sinalização BDNF/TrkB na etiologia do Autismo: análise genética." (THESIS/DISSERTATION)
 18/20
2005/11/28
Concluded
 Biology (Licenciatura)
Universidade de Évora, Portugal
"Estudo da dinâmica populacional do nemátode da madeira do pinheiro, Bursaphelenchus xylophilus, em pinheiro-bravo, Pinus pinaster." (THESIS/DISSERTATION)
 14/20
Affiliation

Science

Category
Host institution 		Employer
2020/08/03 - Current Researcher (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2017/07/01 - 2019/12/31 Postdoc (Research) Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2010/05/01 - 2012/12/31 Research Trainee (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2008/08/01 - 2010/03/31 Research Trainee (Research) Instituto Nacional de Saúde Doutor Ricardo Jorge IP, Portugal
Instituto Nacional de Saúde Doutor Ricardo Jorge IP, Portugal
2006/05/01 - 2006/09/30 Research Trainee (Research) Instituto das Florestas e Conservação da Natureza, Portugal
Brisa, Portugal

Others

Category
Host institution 		Employer
2013/02/01 - 2017/05/31 PhD student fellowship (SFRH/BD/84541/2012) at NemaLab (PT) and The James Hutton Institute (UK) Fundação para a Ciência e a Tecnologia, Portugal
The James Hutton Institute, United Kingdom
(...)
Projects

Grant

Designation Funders
2021/03/01 - Current PratyOmics - Exploring plant metabolome for the control of the root-lesion nematode Pratylenchus penetrans
PTDC/ASP-PLA/0197/2020
Researcher
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/01 - Current D4Ss - Food-web approaches to assess the functional benthic ecosystem interactions for Marine and Coastal management under the Marine Strategy Framework Directive
ALT20-03-0145-FEDER-029400
Researcher
Universidade de Évora, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2020 - Current PineENEMY - Exploring the NEmatode-MYcobiota interactions in Pine Wilt Disease
PTDC/ASP-PLA/28724/2017
Researcher
Universidade de Évora, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01 - 2025/01 NemaWAARS - A motif to unveil mechanisms of parasitism gene regulation in the pinewood nematode as a target for disease control and plant resistance.
PTDC/ASP-PLA/1108/2021
Principal investigator
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2013/01 - 2017/05 Functional characterization of putative effectors in pinewood nematode, Bursaphelenchus xylophilus.
SFRH/BD/84541/2012
 Fundação para a Ciência e a Tecnologia
2016 - 2016 Pharyngeal gland cells sequencing – new insights into the effector proteins required for nematode parasitism.
ICAAM - Pharyngeal gland cells sequencing
Researcher
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
 Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas
Concluded
2013 - 2015 MICRONEMA - Análise espacial e temporal das comunidades microbianas na doença do pinheiro/ Spatial and temporal analysis of microbial community in Pine Wilt Disease.
PTDC/BIA-MIC/3768/2012
Researcher
Universidade de Évora, Portugal

Universidade de Aveiro, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2014 - 2014 Characterization of candidate parasitism genes in the pinewood nematode, Bursaphelenchus xylophilus.
ICAAM - Characterization of candidate parasitism genes in the pinewood nematode, Bursaphelenchus xylophilus.
Researcher
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
 Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas
Concluded

Contract

Designation Funders
2020/08/02 - 2026/08/01 A biotechnology approach to develop nematode resistance in forestry species.
CEECIND/00066/2018
Principal investigator
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018/05/01 - 2019/09/30 PratyTech - Biotechnology approaches towards the control of the root lesion nematode Pratylenchus penetrans
PTDC/AGR-PRO/2589/2014
Post-doc Fellow
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2013 - 2017 Functional characterization of putative effectors in pinewood nematode, Bursaphelenchus xylophilus.
SFRH/BD/84541/2012
PhD Student Fellow
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal

The James Hutton Institute, United Kingdom
Concluded
2011 - 2014 REPHRAME - Seventh Framework Programme “Development of improved methods for detection, control and eradication of pinewood nematode in support of EU Plant Health policy.
FP7-KBBE-2010-4.09
Research Fellow
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
Concluded
2009 - 2010 AFN - O nemátode da madeira do pinheiro (Autoridade Florestal Nacional/FFP)
AFN
Research Fellow
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
Concluded

Other

Designation Funders
2017/09 - 2017/09 Participation in SPIT (Secretions Produced In Tylenchida) - scientific network
SPIT Meeting - scientific network meeting
Other
2017 - 2017 Transcriptomic and genomic approaches towards the control of the virus vector nematode Xiphinema index.
NON-FUNDED
Researcher
2014 - 2014 Unraveling pinewood nematode pathogenicity: An integrative and comparative approach
NON-FUNDED
Researcher
2008 - 2009 The Autism Genome Project - Portugal
The Autism Genome Project - Autism Speaks
Master Student Fellow
Concluded
Outputs

Publications

Book
  1. Espada, Margarida; Shynia, Ryoji; Li, Hongmei; Filipiak, Anna; Vicente, Cláudia S L. Corresponding author: Espada, Margarida. Global Occurrence of Pine Wilt Disease: Biological Interactions and Integrated Management. Frontiers Media SA. 2022.
    10.3389/978-2-88976-867-7
  2. Espada, Margarida. Adão, H., Vicente, CSL., Sroczynska, K., Espada, M., Alvim, P., Costa, M., Vieira, S. Book of Abstracts, SeventIMCO - Seventeenth International Meiofauna Conference. Évora, Portugal. 2019.
    Published
Book chapter
  1. "Mota, M., Vicente, C., Espada, M., Vieira, P., Chastagner, G. Pine wilt". In Compendium of conifer diseases. United States: APS Press, 2018.
    Published
  2. Valadas, V.; Espada, M.; Nobre, T.; Mota, M.; Arnholdt-Schmitt, B.. "AOX in parasitic nematodes: A matter of lifestyle?". In Alternative respiratory pathways in higher plants. John Wiley & Sons, Ltd, 2015.
    Published
Conference abstract
  1. "Vicente, C, Espada, M, Mota, M, Vieira, P. Understanding the role and delivery of Pratylenchus penetrans effectors proteins.". Paper presented in International meeting of the Portuguese society of genetics, Faro, 2020.
  2. "M. Mota, C. Vicente, J. Branco, M. Espada, P. Vieira. Plant-nematode interactions: recent advances in the control of a serious pest and pathogen, the root lesion nematode, Pratylenchus penetrans. M. Mota, C. Vicente, J. Branco, M. Espada, P. Vieira.". Paper presented in 2ndAnnual congress on plant science and biosecurity, London, 2019.
  3. "M. Mota, M. Espada, C. Vicente. Pinewood nematode-microbe-plant-insect interactions, at a molecular level.". Paper presented in 1st International Molecular Plant Protection Congress, Adana, 2019.
  4. "Nascimento F, Cláudia SL Vicente, Barbosa P, Espada M, Vieira P, Hasegawa K, Mota M. Ecological role of bacteria associated with the pine wilt disease system. Nascimento F, Cláudia SL Vicente, Barbosa P, Espada M, Vieira P, Hasegawa K, Mota M.". Paper presented in 6th International Congress of Nematology, Cape Town, 2014.
  5. "Cláudia SL Vicente, Nascimento F, Espada M, Barbosa P, Hasegawa K, Mota M, Oliveira S. Natural bacterial communities associated with pine sawyer beetle monochamus galloprovincialis.". Paper presented in IUFRO Pine wilt disease conference, Braunschweig, 2013.
  6. "Mallez S., Castagnone C., Espada M., Mota M., Guillemaud T., Castagnone-Sereno P. Development of Bursaphelenchus xylophilus-specific microsatellite markers to assess the genetic diversity of populations from European forests.". Paper presented in 31st ESN Symposium, Adana, 2012.
  7. "Mallez S., Castagnone C., Espada M., Vieira P., Mota M., Castagnone-Sereno P., Guillemaud T. Invasion du nématode du pin, Bursaphelenchus xylophilus - Développement d¿outils et premières analyses.". Paper presented in 34ème réunion annuelle du Groupe d’Etude de Biologie et Génétique des Populations, Avignon, 2012.
  8. "Mota, M., M. Espada, C. Vicente, P. Vieira, R. Souza. Bursaphelenchus xylophilus and B. cocophilus: Opportunities and perspectives in the study of two nematodes causing two serious forest diseases: Pine wilt disease and red ring disease of coconut.". Paper presented in 30º Congresso Brasileiro de Nematologia, 2012.
  9. "F.X. Nascimento, C. Vicente, M. Espada, M. Mota. Perspectives on the use of plant growth promoting bacteria as biocontrol agents for the pine wilt disease.". Paper presented in Symposium & Inauguration of the Centre for Biological Control (CBC), Uppsala, 2012.
  10. "Cláudia Vicente, Francisco Nascimento, Margarida Espada, Pedro Barbosa, Manuel Mota, and S. Oliveira. Are Bursaphelenchus xylophilus associated bacteria playing a role in pine wilt disease?". Paper presented in JNS Meeting, Tsukuba, 2012.
  11. "V. Valadas, P. Barbosa, M. Espada, S. Oliveira, M. Mota. The pinewood nematode, Bursaphelenchus xylophilus, in Portugal: possible introductions and spread routes of a serious biological invasion revealed by molecular methods.". Paper presented in XLIII ONTA Annual Meeting, Coimbra, 2011.
  12. "C. Vicente, F.X. Nascimento, M. Espada, S. Oliveira, M. Mota. Dual role of bacteria in Pine Wilt Disease.". Paper presented in COST Action 872 e 63th International Symposium on Crop Protection, Ghent, 2011.
  13. "Magalhães, TR, Casey, J., Correia, C., Sequeira, F., Espada, M., Ennis, S., Vicente, AM. Genome-Wide Association Analysis of Susceptibility Genes in Autism Using with a Gene-Centric Approach.". Paper presented in AGProject. International Meeting for Autism Research, Philadelphia, 2010.
Conference poster
  1. "Espada M, Vicente CLS, Branco J, Mota M, Vieira P. corresponding author: M. Espada. The role of Pratylenchus penetrans transthyretin-like protein in parasitism.". Paper presented in Internacional Symposium of crop protection, Ghent, 2021.
  2. "C. Vicente, H. Bragança, L. Bonifácio, E. Sousa, M. Espada, M. Mota, F. Nóbrega, M.L. Inácio. Mycobiota of Pinus pinaster associated with Pine Wilt Disease.". Paper presented in Advances in Nematology Conference, Association of Applied Biologists, London, 2020.
  3. "M. Espada, C.Vicente, J. Branco, M. Mota, P. Vieira. corresponding author: M. Espada. Characterisation of transthyretin-like proteins of the plant-parasitic nematode Pratylenchus penetrans suggests involvement in oxidative stress response". Paper presented in IS-Molecular Plant-microbe interaction XVIII Congress 2019, Glasgow, 2019.
  4. "C. Vicente, J. Branco, M. Espada, P. Barbosa, P. Vieira, A. Cristina Figueiredo, M. Mota. Investigação no controlo dos nemátodos das lesões radiculares na cultura de batata.". Paper presented in VI Jornadas Técnicas da batata, Santarém, 2019.
  5. "Silva, A.C, Espada, M., Jones, J.T., Mota, M. Characterisation of the pharyngeal gland cells of Bursaphelenchus xylophilus- from morphometrics to molecular biology". Paper presented in 47th Organization of nematologists for tropical America annual meeting, Varadero, 2015.
  6. "M. Espada, C. Vicente, F. Nascimento, P. Barbosa, M. Mota. corresponding author: M Espada. An integrative approach on Pine wilt disease". Paper presented in 1º Simpósio SCAP- 7º Simpósio SPF, Novos desafios na protecção das plantas, Oeiras, 2014.
  7. "Mallez, S, castagnone, C, Espada, M, Vieira, P, Eisenback, J, Mota, M, Aikawa, T, Akiba, M, Kosaka, H, Castagnone-Sereno, P. First insights into the genetic diversity of the pinewood nematode in its native area and around the world.". Paper presented in XIV Congress of the European society for evolutionary biology, Lisboa, 2013.
  8. "Vieira, P, Castagnone, C., Mallez, S, Espada, M., Navas, A, Mota, M, Castagnone-Sereno, P. Sequence Variability of The MspI Satellite DNA family Of The Pinewood Nematode At Different Geographic Scales.". Paper presented in Society of Nematologists annual meeting 2013, Knoxville, 2013.
  9. "Nascimento F.X., Espada, M., Vicente, C. S. L., Glick, B.R., Mota, M. Pseudomonas putida UW4 producing ACC deaminase is a potential biocontrol agent for pine wilt disease". Paper presented in 31st European Society of Nematologists Symposium, 2012.
  10. "Vicente C, Nascimento F, Espada M, Barbosa P, Mota M, Glick B, Oliveira S. Heterogeneous microbial community associated with Bursaphelenchus xylophilus.". Paper presented in 31st European Society of Nematologists, Adana, 2012.
  11. ". M. Mota, P. Vieira, A.C. Penas, P. Roque, M. Fonseca, V. Valadas, F. Figo, M. Espada, A.R. Santos. Bursaphelenchus xylophilus: uma séria ameaça aos ecossistemas florestais em Portugal". Paper presented in II Congresso Nacional Ordem dos Biólogos, Porto, 2005.
Journal article
  1. Espada, Margarida; Filipiak, Anna; Li, Hongmei; Shinya, Royji; Vicente, Cláudia S L. Corresponding author: Espada, Margarida. "Editorial: Global occurrence of pine wilt disease: Biological interactions and integrated management". Frontiers in Plant Science 13:993482 (2022):
    Open access • Accepted
  2. Vicente, Cláudia S L; Soares, Miguel; Faria, Jorge; Espada, Margarida; Mota, Manuel Galvão de Melo e; Nobrega, Filomena ; Inacio, Maria. "Fungal communities of the Pine Wilt Disease complex – studying the interaction of Ophiostomatales with Bursaphelenchus xylophilus.". Frontiers Plant Science (2022):
    Open access • Accepted
  3. Espada, M.; Eves-van den Akker, S.; Maier, T.; Paramasivan, V.; Baum, T.; Mota, M.; Jones, J.T.. "STATAWAARS: A promoter motif associated with spatial expression in the major effector-producing tissues of the plant-parasitic nematode Bursaphelenchus xylophilus". BMC Genomics 19 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85050598566&partnerID=MN8TOARS.
    10.1186/s12864-018-4908-2
  4. Nascimento, F.X.; Espada, M.; Barbosa, P.; Rossi, M.J.; Vicente, C.S.L.; Mota, M.. "Non-specific transient mutualism between the plant parasitic nematode, Bursaphelenchus xylophilus, and the opportunistic bacterium Serratia quinivorans BXF1, a plant-growth promoting pine endophyte with antagonistic effects". Environmental Microbiology (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-85005922674&partnerID=MN8TOARS.
    10.1111/1462-2920.13568
  5. Espada, M.; Jones, J.T.; Mota, M.. "Characterization of glutathione S-transferases from the pine wood nematode, Bursaphelenchus xylophilus". Nematology 18 6 (2016): 697-709. http://www.scopus.com/inward/record.url?eid=2-s2.0-84982860375&partnerID=MN8TOARS.
    10.1163/15685411-00002985
  6. Tsai, I.J.; Tanaka, R.; Kanzaki, N.; Akiba, M.; Yokoi, T.; Espada, M.; Jones, J.T.; Kikuchi, T.. "Transcriptional and morphological changes in the transition from mycetophagous to phytophagous phase in the plant-parasitic nematode Bursaphelenchus xylophilus". Molecular Plant Pathology 17 1 (2016): 77-83. http://www.scopus.com/inward/record.url?eid=2-s2.0-84957840543&partnerID=MN8TOARS.
    10.1111/mpp.12261
  7. Espada, M.; Silva, A.C.; Eves Van Den Akker, S.; Cock, P.J.A.; Mota, M.; Jones, J.T.. "Identification and characterization of parasitism genes from the pinewood nematode Bursaphelenchus xylophilus reveals a multilayered detoxification strategy". Molecular Plant Pathology 17 2 (2016): 286-295. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956575961&partnerID=MN8TOARS.
    10.1111/mpp.12280
  8. Mallez, S.; Castagnone, C.; Espada, M.; Vieira, P.; Eisenback, J.D.; Harrell, M.; Mota, M.; et al. "Worldwide invasion routes of the pinewood nematode: What can we infer from population genetics analyses?". Biological Invasions 17 4 (2015): 1199-1213. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925467104&partnerID=MN8TOARS.
    10.1007/s10530-014-0788-9
  9. Vieira, P.; Castagnone, C.; Mallez, S.; Espada, M.; Navas, A.; Mota, M.; Castagnone-Sereno, P.. "Sequence variability of the MspI satellite DNA family of the pinewood nematode Bursaphelenchus xylophilus at different geographic scales". Molecular Phylogenetics and Evolution 70 1 (2014): 120-129. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885335280&partnerID=MN8TOARS.
    10.1016/j.ympev.2013.09.017
  10. Mallez, S.; Castagnone, C.; Espada, M.; Vieira, P.; Eisenback, J.D.; Mota, M.; Guillemaud, T.; Castagnone-Sereno, P.. "First Insights into the Genetic Diversity of the Pinewood Nematode in Its Native Area Using New Polymorphic Microsatellite Loci". PLoS ONE 8 3 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84875048424&partnerID=MN8TOARS.
    10.1371/journal.pone.0059165
  11. Nascimento, F.X.; Vicente, C.S.L.; Barbosa, P.; Espada, M.; Glick, B.R.; Mota, M.; Oliveira, S.. "Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus". BioControl 58 3 (2013): 427-433. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878213939&partnerID=MN8TOARS.
    10.1007/s10526-012-9500-0
  12. Vicente, C.S.L.; Nascimento, F.X.; Espada, M.; Barbosa, P.; Hasegawa, K.; Mota, M.; Oliveira, S.. "Characterization of bacterial communities associated with the pine sawyer beetle Monochamus galloprovincialis, the insect vector of the pinewood nematode Bursaphelenchus xylophilus". FEMS Microbiology Letters 347 2 (2013): 130-139. http://www.scopus.com/inward/record.url?eid=2-s2.0-84884715484&partnerID=MN8TOARS.
    10.1111/1574-6968.12232
  13. Vicente, C.; Espada, M.; Vieira, P.; Mota, M.. "Pine Wilt Disease: A threat to European forestry". European Journal of Plant Pathology 133 1 (2012): 89-99. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859108651&partnerID=MN8TOARS.
    10.1007/s10658-011-9924-x
  14. Vicente, C.S.L.; Nascimento, F.; Espada, M.; Barbosa, P.; Mota, M.; Glick, B.R.; Oliveira, S.. "Characterization of Bacteria Associated with Pinewood Nematode Bursaphelenchus xylophilus". PLoS ONE 7 10 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84867593794&partnerID=MN8TOARS.
    10.1371/journal.pone.0046661
  15. Ribeiro, B.; Espada, M.; Vu, T.; Nóbrega, F.; Mota, M.; Carrasquinho, I.. "Pine wilt disease: Detection of the pinewood nematode (Bursaphelenchus xylophilus) as a tool for a pine breeding programme". Forest Pathology 42 6 (2012): 521-525. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870692545&partnerID=MN8TOARS.
    10.1111/efp.12010
  16. Vicente, C.; Espada, M.; Vieira, P.; Mota, M.. "Erratum to Pine Wilt Disease: A threat to European forestry (Eur J Plant Pathol, 10.1007/s10658-011-9924-x)". European Journal of Plant Pathology 133 2 (2012): 497-497. http://www.scopus.com/inward/record.url?eid=2-s2.0-84860167487&partnerID=MN8TOARS.
    10.1007/s10658-012-9979-3
  17. Valadas, V.; Laranjo, M.; Barbosa, P.; Espada, M.; Mota, M.; Oliveira, S.. "The pine wood nematode, Bursaphelenchus xylophilus, in Portugal: Possible introductions and spread routes of a serious biological invasion revealed by molecular methods". Nematology 14 8 (2012): 899-911. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873693260&partnerID=MN8TOARS.
    10.1163/156854112X632673
  18. Vicente, C.S.L.; Nascimento, F.; Espada, M.; Mota, M.; Oliveira, S.. "Erratum: Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal (Antonie van Leeuwenhoek (2011) 100 (477-481) DOI: 10.1007/s10482-011-9602-1)". Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 101 4 (2012): 923-923. http://www.scopus.com/inward/record.url?eid=2-s2.0-84862659254&partnerID=MN8TOARS.
    10.1007/s10482-012-9732-0
  19. Vicente, C.S.L.; Nascimento, F.; Espada, M.; Mota, M.; Oliveira, S.. "Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal". Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 100 3 (2011): 477-481. http://www.scopus.com/inward/record.url?eid=2-s2.0-80054091650&partnerID=MN8TOARS.
    10.1007/s10482-011-9602-1
Magazine article
  1. Espada, Margarida; Mendonça, Madalena; Mota, Manuel Galvão de Melo e; Inacio, Maria. Corresponding author: Espada, Margarida. "Parasitismo no nemátode da madeira do pinheiro – Estudo dos mecanismos de regulação de genes para o controlo da doença.", AgroTec, 2022, http://hdl.handle.net/10174/33636.
Thesis / Dissertation
  1. Espada, Margarida. "Functional characterization of putative effectors in pinewood nematode, Bursaphelenchus xylophilus.". PhD, Universidade de Évora, 2018.
  2. Espada, Margarida. "Envolvimento da via de sinalização BDNF/TrkB na etiologia do autismo: análise genética.". Master, Universidade de Lisboa Faculdade de Ciências, 2010.
  3. Espada, Margarida. "Estudo da dinâmica populacional do nemátode da madeira do pinheiro, Bursaphelenchus xylophilus, em pinheiro-bravo, Pinus pinaster.". Degree, Universidade de Évora, 2005.
Website
  1. Espada, M. Responsible for creating and updating the website of the Laboratory of Nematology (NemaLab), at MED.. 2017. https://www.icaam.uevora.pt/NemaLab.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/06 Transcriptomic approach as a tool for identification of pinewood nematode parasitism-related genes. PWN-Pinaster (Pine Wilt Disease: recent advances in molecular analyses and breeding approaches)
Universidade de Lisboa, Faculdade de ciências (Portugal)
2022/05 NemaWAARS: A motif to unveil mechanisms of parasitism gene regulation in the pinewood nematode as a target for disease control and plant resistance. International Conference Nematologists 22
(Juan-le-Pins, France)
2018/09 STATAWAARS: a new promoter DNA motif associated with pharyngeal gland cell expression in Bursaphelenchus xylophilus. 33rd Symposium European Society of Nematologists
European Society of Nematologists (Ghent, Belgium)
2018/04/18 Interação nemátode-planta: abordagem transcriptómica revela novos genes de parasitismo no nemátode da madeira-do pinheiro, Bursaphelenchus xylophilus. 6º Edição - INVESTIGAR-ICAAM - Um olhar sobre as interações multi-tróficas em ecossistemas do Mediterrâneo
ICAAM (Évora, Portugal)
2017/11 A transcriptomic approach reveals new parasitism genes in the pinewood nematode, Bursaphelenchus xylophilus. II PhD Students meeting in environment agriculture
Universidade de Évora (Évora, Portugal)
2017/06/21 Identification of new parasitism proteins of the pinewood nematode, Bursaphelenchus xylophilus 15th Congress of the Mediterranean Phytopathological Union
Mediterranean Phytopathological Union (Cordoba, Spain)
2016/08/29 Pinewood nematode effectors: different approaches to predict new genes involved in parasitism 32nd Symposium European Society of Nematologists
European Society of Nematologists (Braga, Portugal)
2015/05/20 Using RNAseq to identify effectors from nematodes. Nematology Seminar day - PINC (Master of Science in Nematology)
International Master of Science in Agro- and Environmental Nematology Master programme (Ghent, Belgium)
2015/05/19 Identification of novel effectors from the pinewood nematode, Bursaphelenchus xylophilus. 67th International Symposium of Crop Protection
Ghent University (Ghent, Belgium)
2014/06 Identification of effectors from Bursaphelenchus xylophilus - an RNAseq approach Dundee Effector Consortium Retreat
Dundee Effector Consortium (Birnam, United Kingdom)
2010/07 Análise das vias biológicas do Autismo Seminários de Actualização Cientifica e Metodológica em Promoção da Saúde e Doenças Crónicas
Instituto Nacional de Saúde Dr Ricardo Jorge (Lisboa, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/11 - 2023/07 Avaliação da expressão relativa de enzimas antioxidantes do nemátode fitoparasita Pratylenchus penetrans envolvidas na resposta ao composto orgânico 3-Octanol.
Co-supervisor
 Licenciatura (Degree)
2022/11 - 2023/07 Validação de genes relacionados com o parasitismo do nemátode da madeira do pinheiro, Bursaphelenchus xylophilus, através de hibridação in situ.
Supervisor
 Licenciatura em Bioquímica (Degree)
2021/11 - 2022 Avaliação do potencial nematodicida de bactérias endófitas no nemátode das lesões radiculares Pratylenchus penetrans.
Co-supervisor of Teresa Allen Monteiro
 Licenciatura em Bioquímica (Degree)
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2021/03/01 - 2021/06 Functional characterization of parasitism genes from pinewood nematode in response to oxidative stress.
Supervisor of Catarina Duarte
 Projecto em Ciencias Biológicas II (Scientific initiation)
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2021/01 - 2021/06 Molecular characterization of parasitism genes from pinewood nematode, Bursaphelenchus xylophilus, in interaction with plant host.
Supervisor of Carolina Mendes Freitas
 Biochemistry (Degree)
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2019/10 - 2020/04 Short scientific training in Nematology
Supervisor of Susana Barbosa
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2019/02/01 - 2019/06/23 Functional characterization of parasitism-related genes from pinewood nematode in response to plant defense compounds. ("Caracterização funcional de genes de parasitismo do nemátode da madeira do pinheiro (Bursaphelenchus xylophilus) e o seu envolvimento no metabolismo de compostos de defesa da planta.")
Supervisor of Susana Barbosa
 Projecto em ciências biológicas II - Biologia (Other)
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2014/01 - 2014/09 Identification of novel effectors from the pinewood nematode, Bursaphelenchus xylophilus.
Co-supervisor of Ana Cláudia Silva
 European Master of Science in Nematology (Erasmus Mundus EUMAINE) (Master)
The James Hutton Institute, United Kingdom

Universidade de Évora, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2021/07/19 - 2021/07/23 Summerschool UÉ’21 – “MultiOmics for beginners: explore the big data” (20horas). For undergraduates, PhD students and researchers. (2021/07 - 2021/07)
Workshop (Co-organisor)
 Universidade de Évora, Portugal
2019/11/22 - 2019/11/22 Workshop Nacional “Nemátodes fitoparasitas da batateira: o nematode das lesões radiculares, Pratylenchus penetrans (projecto PratyTech”) INIAV-Oeiras (2019/11/22 - 2019/11/22)
Workshop (Member of the Organising Committee)
 Universidade de Évora, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal
2019/07/07 - 2019/07/12 17th SeventIMCO (International Meiofauna Conference), Évora (Scientific and organizing committee) (2019/07/07 - 2019/07/12)
Congress (Member of the Scientific Committee)
 Universidade de Évora, Portugal
2010/05/24 - 2010/05/27 COST872 NEMAGENICS Lisbon (2010/05/24 - 2010/05/27)
Congress (Member of the Organising Committee)
 Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2020/12/15 - Current Attendee at SON - Society of Nematologists- USA. (webinar)
Conference
 SON - Society of Nematologists- USA
2020/12/15 - Current AAB-Advances in Nematology 2020 (one day webinar)
Conference
Association of Applied Biologists, United Kingdom
2021/12 - 2021/12 Attendee at AAB-Advances in Nematology 2021 (one day webinar)
Meeting
Association of Applied Biologists, United Kingdom
2021/11 - 2021/11 Attendee at IUFRO - Pine wilt disease symposium (online). INRAE, France
Symposium
2021/05/18 - 2021/05/18 Attendee at Internacional Symposium of crop protection - poster communication.
Symposium
 Internacional Symposium of crop protection
Universiteit Gent Universiteitsbibliotheek Gent, Belgium
2019/07/14 - 2019/07/18 2019 IS-MPMI XVIII Congress
Congress
2019/06/27 - 2019/06/28 I Jornadas MED
Conference
 I Jornadas MED
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2019/03/28 - 2019/03/28 Jornadas técnicas da batata
Meeting
 Jornadas técnicas da batata
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2018/09/09 - 2018/09/13 33rd Symposium of European Society of Nematology
Congress
 33rd Symposium of European Society of Nematology
2017/12/19 - 2017/12/19 I Seminário e workshop: A técnica fish (Fluorescence In situ hybridization). Fundamentos, Evolução e Aplicações na análise microbiológica em geral e no Património em particular
Seminar
 A técnica fish (Fluorescence In situ hybridization). Fundamentos, Evolução e Aplicações na análise microbiológica em geral e no Património em particular
Universidade de Évora, Portugal
2013 - 2017 Participation in Dundee Effector consortium (The James Hutton Institute, UK)
Meeting
 Dundee effector retreat
The James Hutton Institute, United Kingdom

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/06 Obtenção e caraterização de biblioteca de mutantes de rizóbio por mutagénese aleatória
(Thesis) Main arguer
 Rute Pereira (Other)
Universidade de Évora Departamento de Química, Portugal
2020/11/02 Epigenetic changes in galls induced by the nematode Meloidogyne javanica in Arabidopsis.
(Thesis) Arguer
 Ana Cláudia Silva (PhD)
Universidad de Castilla-La Mancha, Spain

Association member

Society Organization name Role
2021 - Current IUFRO¿ - International union of forest research organizations Representing MED- University of Évora at IUFRO, Division 7 (Forest health 7.02.00 Pathology; 7.02.10 Pine wilt disease)
2011 - Current European Society of Nematologists

Committee member

Activity description
Role 		Institution / Organization
2020/01 - Current Member of scientific commitee at MED
Member
 Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2018/03 - 2019/12 Member of scientific committee at ICAAM
Member
 Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal

Conference scientific committee

Conference name Conference host
2019/06/28 - 2019/06/28 I JORNADAS MED ¿ Horticulture session (chair) MEDITERRANEAN INSTITUTE FOR AGRICULTURE, ENVIRONMENT AND DEVELOPMENT

Consulting

Activity description Institution / Organization
2020/08 - Current Identification of plant-parasitic pinewood nematode from National forest surveys (ICNF) Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2011 - Current Molecular diagnostic/identification of plant-parasitic nematodes (crops and forestry systems) Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2021/10 - Current Cell biology (Licenciatura) Universidade de Évora Departamento de Biologia, Portugal
2021 - Current Cell biology and hereditary Psicologia Universidade de Évora Departamento de Biologia, Portugal
2022/03 - 2023/03 Fundamentos Preparatórios de Biologia. Curso Preparatório para a Ingressão no Ensino Superior (Outros) Universidade de Évora, Portugal
2021/07/22 - 2021/07/22 "Transcriptomics". Summer school course (SS UÉ'21) MultiOmics analysis in Big data. (Outros) Universidade de Évora, Portugal
2021/01/25 - 2021/01/26 Molecular and morphological diagnostic methods for the identification of the pinewood nematode. Training course for the Phytosanitary authorities of Morocco. Training in molecular diagnostic of the pinewood nematode Universidade de Évora, Portugal
2011 - 2012 Phytonematology , experimental classes. European Master of Science in Nematology European Masters in Nematology (Master) Universidade de Évora, Portugal

Universiteit Gent, Belgium

Universität Bielefeld, Germany

Universidad de Jaén, Spain
2010/06/22 - 2010/06/30 Curso “Caracterização molecular de nemátodes fitoparasitas de Portugal”, realizado no Laboratório de Nematologia, Universidade de Évora Caracterização molecular de nemátodes fitoparasitas de Portugal (Outros) Universidade de Évora, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2023 - Current New Phytologist (Reviewer) (1469-8137) Wiley-Blackwell
2022 - Current BMC Plant Biology (Reviewer) (1471-2229) Springer Nature
2021 - Current Plos Pathogens (Reviewer) (1553-7366) Plos
2021 - Current International Journal of Molecular Sciences (Reviewer) (1422-0067) MDPI
2020/09 - Current Frontiers in Plant Science (Review Editor) (1664-462X) Frontiers Media SA
2020/09 - Current Frontiers in Microbiology (Reviewer) (1664-302X) Frontiers
2019/12 - Current PeerJ (Reviewer) (2167-8359) PeerJ
2018 - Current Forest Pathology (Reviewer) (1439-0329) Wiley (Blackwell Publishing)
2021/05/31 - 2022/02/17 Frontiers in Plant Science, Plant-pathogens interactions (Guest Associate Editor in Research Topic) (1664-462X) Frontiers
2021/03 - 2021/12 Plants - Plant molecular biology section (Special issue editor) (2223-7747) MDPI
2017 - 2017 Journal of Nematology (Reviewer) (0022-300X) Exeley Inc.

Mentoring / Tutoring

Topic Student name
2021/11 - 2022 Molecular biology Marina Costa

Other jury / evaluation

Activity description Institution / Organization
2022/02 - 2022/03 Jury of Master's fellowship (NemaWAARS - A motif to unveil mechanisms of parasitism gene regulation in the pinewood nematode as a target for disease control and plant resistance - PTDC/ASP-PLA/1108/2021). Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
Distinctions

Award

2011 Ideias de Negócio Atrevo.me'10 - Innovative enterprise award
Universidade de Évora, Portugal

Other distinction

2019 Junior Researcher contract- Individual call for scientific employment stimulus 2018
Fundação para a Ciência e a Tecnologia, Portugal
2015 Funding for training at The Baum Lab, Iowa State University, USA | Apoio financeiro para ação de formação e deslocação à Iowa State University (Thomas Baum Lab), EUA, no âmbito do trabalho de doutoramento.
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2013 Funding for Bioinformatic trainee at The James Hutton Institute, Dundee, Scotland | Apoio financeiro para ação de formação/treino em bioinformática e deslocação ao The James Hutton Institute, Escócia, no âmbito do trabalho de doutoramento
Universidade de Évora Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Portugal
2012 PhD fellowship (SFRH/BD/84541/2012)
Fundação para a Ciência e a Tecnologia, Portugal