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I am a molecular microbiologist, specialized in S. aureus cell and molecular biology, cell wall biosynthesis and mechanisms of antimicrobial resistance (PhD in Molecular Biology, FCT-NOVA /2019). My main research interests are in bacterial pathogenesis and molecular bacteriology namely: host-pathogen interaction, intracellular persistence, antimicrobial resistance mechanisms, stringent response, genetic competence and mechanobiology using advanced microscopy approaches such as Super-resolution Structured Illumination Microscopy (SIM) and microphysiological systems as Lung-on-Chip technology. Currently, I am a researcher at the Intracellular Microbial Infection Biology (IMIB) Laboratory (P.I.: Pedro M. Pereira), hosted at Instituto de Tecnologia Química e Biológica (ITQB-NOVA). I have performed postdocs at the Molecular Microbiology of Bacterial Pathogens Lab (P.I.: R. Sobral), at Laboratory of Molecular Genetics (P.I.: H. de Lencastre) and at Microbial Stress Lab (P.I.: S. Pauleta). During these postdocs I was responsible for the introduction on new research lines in the host labs such as the production of bacterial cellulose for wound dressing applications and the development of copper and silver NHC conjugated compounds as antimicrobials against several pathogens. I have authored 11 peer-reviewed journal articles, 7 conference proceedings and 2 book chapters, among which 15 as first-author with a total of 903 citations (Google scholar). I have participated in 15 research grants both from national and international funding agencies, three as a Principal Investigator. My work was presented more than 40 communications selected from abstracts (oral and panel) in national and international conferences and in seminars by invitation. I have extensive experience in supervision of students with a total of 19 supervisions and co-supervisions of national and international students from BSc and MSc degrees, Erasmus program and PhD and MSc laboratory rotations. I am a lecturer at the MolBioS PhD program at ITQB-NOVA. I was assistant in the module "Introduction to Microbiology, Microbial Genetics and Recombinant DNA Technology in Prokaryotes and Eukaryotes" of the Medical Microbiology Master and I was teaching assistant of the disciplines "Biochemistry of Foods" and "Chemistry and Biochemistry of Foods" at NOVA-FCT. I am an ad-hoc reviewer for PeerJ, PLOS ONE and the European Journal of Microbiology and Biotechnology. I am part of the Editorial Board of the International Journal of Microbiology and Biotechnology, and I have participated in 19 jury committees as main opponent in MSc, post-graduations, and BSc examinations. I was also an evaluator of three international grants (Latvia and Australia) and have participated as member of different evaluation panels and committees. I serve as Member of the MPS_NOVA Twinning Research Committee. I have participated in dissemination activities such as the ITQB-open day, Expo-FCT, European Researcher's night and the Feira Internacional de Ciência (FIC.A). I was also member of the Task Force for COVID-19, performing RT-PCR testing during the covid pandemics. I was awarded an ESCMID research grant as PI in 2022. In 2023, I secured the highly competitive FCT/MCTES CEEC Individual Call to Scientific Employment Stimulus for the project PERSISTAUREUS (Ref.: 2023.06402.CEECIND/CP2836/CT0008). In 2025, I was awarded the MPS_NOVA TWINNING (European Commission) grant as PI for the project Modelling Bacterial Pneumonia Infection Dynamics in Lung-on-Chip. Later that year, I received an ECRA (Early Career Researcher Award) grant as PI, in collaboration with M. del Rosario, for the project Monitoring Infection-Induced Cellular Stress Using PhotoFiTT in Staphylococcus aureus Models.
Identification

Personal identification

Full name
Raquel Pereira Portela

Citation names

  • Portela, R.

Author identifiers

Ciência ID
1813-219B-DD6E
ORCID iD
0000-0002-5559-9554
Google Scholar ID
https://scholar.google.com/citations?user=mQs01oUAAAAJ&hl=en
Researcher Id
L-3264-2014
Scopus Author Id
57201234562

Email addresses

  • raquel.portela@itqb.unl.pt (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Microbiology
Education
Degree Classification
2019/12/16
Concluded
Biologia (Doutoramento)
Major in Biologia Molecular
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"New studies in the resistance to ß-lactams and in cell wall damage survival in Staphylococcus aureus: an integrated approach." (THESIS/DISSERTATION)
Affiliation

Science

Category
Host institution
Employer
2025/06 - 2025/07 Visiting Researcher (Research) Universitätsklinikum Jena, Germany

Others

Category
Host institution
Employer
2024/10/01 - Current PhD Researcher under contract ref:2023.06402.CEECIND/CP2836/CT0008 "PERSISTAUREUS - Unravelling the mechanisms of S. aureus antibiotic induced persistence during intracellular host-cell infection" Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Universidade Nova de Lisboa Microbiologia Molecular Estrutural e Celular, Portugal
(...)
Projects

Grant

Designation Funders
2025/06 - Current Monitoring Infection-Induced Cellular Stress Using PhotoFiTT in Staphylococcus aureus Models
ECRA 2025
Co-Principal Investigator (Co-PI)
Ongoing
2025/03 - Current Modelling Bacterial Pneumonia Infection Dynamics in Lung-on-chip: A Comparative Study of Legionella pneumophila and Staphylococcus aureus
MPS_NOVA Exploratory Research Grant
Principal investigator
European Commission
Ongoing
2022/04/01 - 2023/02/28 Characterization and Application of a New BacteriocinSystem of Staphylococcus aureus. (ESCMID Research Grant 2022).
ESCMID Research Grant 2022
Principal investigator
European Society of Clinical Microbiology and Infectious Diseases
Concluded

Contract

Designation Funders
2024/10/01 - Current 2023.06402.CEECIND - PERSISTAUREUS - Unravelling the mechanisms of Staphylococcus aureus antibiotic induced persistence during intracellular host-cell infection.
Principal investigator
Fundação para a Ciência e a Tecnologia
Outputs

Publications

Book chapter
  1. Portela, R.; Borovnik, K.; Costa, C.; Grilo, I. R.; Pereira, P. M.. "High priority pathogens. Where do we stand?". In Nanotherapeutics for Infectious Diseases. Jenny Stanford Publishing Pte Ltd. ISBN-9789815129366, 2024.
    Published
  2. Portela, R.; Almeida, P.L.; Sobral, R. G.; Leal, C. R.. Corresponding author: Leal, C. R.. "S. aureus and E. coli Co-culture Growth Under Shear". In Springer Proceedings in Materials, 108-112. Springer 10.1007/978-3-030-27701-7_23, 2019.
    Published
Journal article
  1. Del Rosario, Mario; Gómez-de-Mariscal, Estibaliz; Morgado, Leonor; Portela, Raquel; Jacquemet, Guillaume; Pereira, Pedro M.; Henriques, Ricardo. "PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments". Nature Communications 16 1 (2025): https://doi.org/10.1038/s41467-025-66209-6.
    10.1038/s41467-025-66209-6
  2. Saraiva, Bruno M.; Cunha, Inês; Brito, António D.; Follain, Gautier; Portela, Raquel; Haase, Robert; Pereira, Pedro M.; Jacquemet, Guillaume; Henriques, Ricardo. "Efficiently accelerated bioimage analysis with NanoPyx, a Liquid Engine-powered Python framework". Nature Methods 22 2 (2025): 283-286. https://doi.org/10.1038/s41592-024-02562-6.
    Published • 10.1038/s41592-024-02562-6
  3. Portela, R.; N. Faria; M. Miragaia; M. Mwangi; H. de Lencastre; A. Tomasz; R. Sobral. "Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus.". Microbiology Spectrum (2022): https://doi.org/10.1128/spectrum.02483-21.
    Published • https://doi.org/10.1128/spectrum.02483-21
  4. Almeida, Ana P.C.; Saraiva, João N.; Cavaco, Gonçalo; Portela, R.; Leal, Catarina R.; Sobral, Rita G.; Almeida, Pedro L.. "Crosslinked bacterial cellulose hydrogels for biomedical applications". European Polymer Journal (2022): 111438. http://dx.doi.org/10.1016/j.eurpolymj.2022.111438.
    Published • 10.1016/j.eurpolymj.2022.111438
  5. Portela, R.; F. Valcovo,; P. L. Almeida, ; R. G. Sobral, ; C. R. Leal.. "Antibiotic Activity Screened by the Rheology of S. aureus Cultures". Fluids 5 2 (2020): 76. http://dx.doi.org/10.3390/fluids5020076.
    Published • 10.3390/fluids5020076
  6. Gonçalves, B. V. *; Portela, R. *; R. Lobo ; T. A. Figueiredo; I. R. Grilo; A. M. Ludovice; H. de Lencastre; J. S. Dias; R. G. Sobral. "Role of MurT C-Terminal Domain in the Amidation of Staphylococcus aureus Peptidoglycan". Antimicrobial Agents and Chemotherapy 63 10 (2019): http://dx.doi.org/10.1128/aac.00957-19 *co-first author.
    Published • 10.1128/aac.00957-19
  7. Portela, R.; C. R. Leal; P. L. Almeida; R. G. Sobral. "Bacterial cellulose: a versatile biopolymer for wound dressing applications". Microbial Biotechnology 12 4 (2019): 586-610. http://dx.doi.org/10.1111/1751-7915.13392.
    Published • 10.1111/1751-7915.13392
  8. Portela, R.; P. L. Almeida; R. G. Sobral; C. R. Leal. "Motility and cell shape roles in the rheology of growing bacteria cultures". The European Physical Journal E 42 3 (2019): http://dx.doi.org/10.1140/epje/i2019-11787-9.
    Published • 10.1140/epje/i2019-11787-9
  9. Portela, R.; Patrício, P.; Almeida, P. L.; Sobral, R. G.; Franco, J. M.; Leal, C. R.. Corresponding author: Leal, C. R.. "Rotational tumbling of Escherichia coli aggregates under shear". Physical Review E 94 6 (2016): http://dx.doi.org/10.1103/physreve.94.062402.
    Published • 10.1103/physreve.94.062402
  10. Patrício, P.; Almeida, P. L.; Portela, R.; Sobral, R. G.; Grilo, I. R.; Cidade, T.; Leal, C. R.. "Living bacteria rheology: Population growth, aggregation patterns, and collective behavior under different shear flows". Physical Review E 90 2 (2014): http://dx.doi.org/10.1103/physreve.90.022720.
    Published • 10.1103/physreve.90.022720
  11. Portela, R.; Almeida, P. L.; Patrício, P.; Cidade, T.; Sobral, R. G.; Leal, C. R.. "Real-time rheology of actively growing bacteria". Physical Review E 87 3 (2013): http://dx.doi.org/10.1103/physreve.87.030701.
    Published • 10.1103/physreve.87.030701
Thesis / Dissertation
  1. Portela, Raquel. "New studies in the resistance to ß-lactams and in cell wall damage survival in Staphylococcus aureus: an integrated approach". PhD, 2019.
Activities

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2025/05 Transform the microscope into an intelligent sensor. HEALTHNEWS journal
Distinctions

Award

2025 MPS_NOVA Exploratory Research Grant
MPS_NOVA TWINNING, Portugal
2025 Early Career Research Award (ECRA)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal
2023 Individual Research Contract - 2023.06402.CEECIND
Fundação para a Ciência e a Tecnologia, Portugal
2022 ESCMID RESEARCH GRANT
European Society of Clinical Microbiology and Infectious Diseases, Switzerland