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11 years’ experience in Health-related Research & Development. Knowledge of biophysical approach to study antimicrobial proteins and blood clot function. Experience in student leadership in laboratory environment. Writing and reviewing competence of scientific papers. (co)Author of 23 scientific research papers and 3 book chapters. Presentation skills confirmed by the oral and poster presentations in international and national conferences.
Identificação

Identificação pessoal

Nome completo
Marco Domingues

Nomes de citação

  • Domingues, Marco

Identificadores de autor

Ciência ID
E317-92F8-84C6
ORCID iD
0000-0003-1502-1421

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Biofísica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Espanhol; Castelhano Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1)
Formação
Grau Classificação
2008/03/01 - 2012/12/19
Concluído
 PhD (Doutoramento)
Universidade de Lisboa Faculdade de Medicina, Portugal
"Study of the mechanism of action of the antimicrobial peptide rBPI21 in Gram-negative bacteria" (TESE/DISSERTAÇÃO)
 Highest Grade
2003/09/01 - 2007/07/01
Concluído
 Biochemistry (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
 17 (out of 20)
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2019/03/01 - 2025/03/01 Assistente de Investigação (carreira) (Investigação) Instituto de Medicina Molecular, Portugal
Instituto de Medicina Molecular, Portugal
2016/11/01 - 2019/02/28 Pós-doutorado (Investigação) Fundação para a Ciência e a Tecnologia, Portugal
Instituto de Medicina Molecular, Portugal
2016/02/01 - 2016/10/01 Pós-doutorado (Investigação) Fundação para a Ciência e a Tecnologia, Portugal
Instituto de Medicina Molecular, Portugal
2013/10/01 - 2016/02/01 Pós-doutorado (Investigação) University of Leeds Faculty of Medicine and Health, Reino Unido
University of Leeds Faculty of Medicine and Health, Reino Unido

Outros

Categoria Profissional
Instituição de acolhimento 		Empregador
2013/02/01 - 2013/08/01 Organization Secretariat of Biophysical Congress, EBSA2013 Portuguese Biophysical Society, Portugal
Projetos

Bolsa

Designação Financiadores
2017/05/01 - 2019/02/28 Delivery and targeting fibrinolytic agents to blood clots
SFRH/BPD/122779/2016
Bolseiro de Pós-Doutoramento
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2015/01/01 - 2018/01/01 Role of extended knob-hole interaction sites in fibrin polymerisation and clot stability
FS/15/37/31513
Bolseiro de Doutoramento
University of Leeds Faculty of Medicine and Health, Reino Unido
 British Heart Foundation
Concluído
2009/09/01 - 2010/09/01 Evaluation of the interaction of antimicrobial peptides with LPS and biomembranes model systems by light scattering methodologies
NA
Bolseiro de Iniciação Científica
Instituto de Medicina Molecular, Portugal
 Universidade de Lisboa Faculdade de Medicina
Concluído

Projeto

Designação Financiadores
2019/03/01 - 2025/03/01 Delivery and targeting of fibrinolytic agents to blood clots
CEECIND/02961/2017
Investigador Pós-doutorado
Instituto de Medicina Molecular, Portugal
Em curso
Produções

Publicações

Artigo em revista
  1. "Nanomechanics of Blood Clot and Thrombus Formation". Annual Review of Biophysics 51 1 (2022): http://dx.doi.org/10.1146/annurev-biophys-111821-072110.
    10.1146/annurev-biophys-111821-072110
  2. "Epigenetic reprogramming by TET enzymes impacts co-transcriptional R-loops". eLife 11 (2022): http://dx.doi.org/10.7554/elife.69476.
    10.7554/elife.69476
  3. "Fibrinogen aC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis". eLife 10 (2021): http://dx.doi.org/10.7554/elife.68761.
    10.7554/elife.68761
  4. "Mechanistic Insights into the Leishmanicidal and Bactericidal Activities of Batroxicidin, a Cathelicidin-Related Peptide from a South American Viper (Bothrops atrox)". Journal of Natural Products 84 6 (2021): 1787-1798. http://dx.doi.org/10.1021/acs.jnatprod.1c00153.
    10.1021/acs.jnatprod.1c00153
  5. Patrícia M. Carvalho; Marcin Makowski; Marco M. Domingues; Ivo C. Martins; Nuno C. Santos. "Lipid membrane-based therapeutics and diagnostics". Archives of Biochemistry and Biophysics 704 (2021): 108858-108858. https://doi.org/10.1016/j.abb.2021.108858.
    10.1016/j.abb.2021.108858
  6. "25-Hydroxycholesterol Effect on Membrane Structure and Mechanical Properties.". International journal of molecular sciences (2021): https://europepmc.org/articles/PMC7961727.
    10.3390/ijms22052574
  7. Figueira, Tiago N.; Domingues, Marco M.; Illien, Françoise; Cadima-Couto, Iris; Todorovski, Toni; Andreu, David; Sagan, Sandrine; et al. "Enfuvirtide-Protoporphyrin IX Dual-Loaded Liposomes: In Vitro Evidence of Synergy against HIV-1 Entry into Cells". ACS Infectious Diseases 6 2 (2019): 224-236. http://dx.doi.org/10.1021/acsinfecdis.9b00285.
    Publicado • 10.1021/acsinfecdis.9b00285
  8. Guedes, Ana Filipa; Carvalho, Filomena A; Domingues, Marco M; Macrae, Fraser L; McPherson, Helen R; Sabban, Aliaa; Martins, Ivo C; et al. "Impact of ¿'¿' fibrinogen interaction with red blood cells on fibrin clots". Nanomedicine 13 19 (2018): 2491-2505. http://dx.doi.org/10.2217/nnm-2018-0136.
    Publicado • 10.2217/nnm-2018-0136
  9. Carvalho, Patrícia M.; Felício, Mário R.; Santos, Nuno C.; Gonçalves, Sónia; Domingues, Marco M.. "Application of Light Scattering Techniques to Nanoparticle Characterization and Development". Frontiers in Chemistry 6 (2018): 237. http://dx.doi.org/10.3389/fchem.2018.00237.
    Publicado • 10.3389/fchem.2018.00237
  10. Guedes AF; Carvalho FA; Domingues MM; Macrae FL; McPherson HR; Santos NC; Ariëns RAS. "Sensing adhesion forces between erythrocytes and gamma' fibrinogen, modulating fibrin clot architecture and function.". Nanomedicine : nanotechnology, biology, and medicine 14 (2018): 909-918. http://europepmc.org/abstract/med/29410160.
    Publicado • 10.1016/j.nano.2018.01.006
  11. Domingues, Marco. "Mechanism of bacterial membrane permeabilization of crotalicidin (Ctn) and its fragment Ctn[15-34], antimicrobial peptides from rattlesnake venom.". Journal of Biological Chemistry 293 (2017): 1536-1549.
    Publicado • 10.1074/jbc.RA117.000125
  12. Dias SA; Freire JM; Pérez-Peinado C; Domingues MM; Gaspar D; Vale N; Gomes P; et al. "New Potent Membrane-Targeting Antibacterial Peptides from Viral Capsid Proteins.". Frontiers in microbiology 8 (2017): 775. http://europepmc.org/abstract/med/28522994.
    Publicado • 10.3389/fmicb.2017.00775
  13. Whyte CS; Chernysh IN; Domingues MM; Connell S; Weisel JW; Ariens RA; Mutch NJ. "Polyphosphate delays fibrin polymerisation and alters the mechanical properties of the fibrin network.". Thrombosis and haemostasis 116 (2016): 897-903. http://europepmc.org/abstract/med/27610454.
    Publicado • 10.1160/th16-01-0062
  14. Macrae FL; Domingues MM; Casini A; Ariëns RA. "The (Patho)physiology of Fibrinogen gamma'.". Seminars in thrombosis and hemostasis 40 (2016): 344-355. http://europepmc.org/abstract/med/27071047.
    Publicado • 10.1055/s-0036-1572353
  15. Domingues, Marco M.; Macrae, Fraser L.; Duval, Cédric; McPherson, Helen R.; Bridge, Katherine I.; Ajjan, Ramzi A.; Ridger, Victoria C.; et al. "Thrombin and fibrinogen gamma' impact clot structure by marked effects on intrafibrillar structure and protofibril packing". Blood 127 4 (2016): 487-495. http://dx.doi.org/10.1182/blood-2015-06-652214.
    Publicado • 10.1182/blood-2015-06-652214
  16. Conceição K; Magalhães PR; Campos SR; Domingues MM; Ramu VG; Michalek M; Bertani P; et al. "The anti-inflammatory action of the analgesic kyotorphin neuropeptide derivatives: insights of a lipid-mediated mechanism.". Amino acids 40 (2016): 307-318. http://europepmc.org/abstract/med/26347373.
    Publicado • 10.1007/s00726-015-2088-9
  17. Faustino AF; Guerra GM; Huber RG; Hollmann A; Domingues MM; Barbosa GM; Enguita FJ; et al. "Understanding dengue virus capsid protein disordered N-Terminus and pep14-23-based inhibition.". ACS chemical biology 10 (2015): 517-526. http://europepmc.org/abstract/med/25412346.
    Publicado • 10.1021/cb500640t
  18. Domingues MM; Silva PM; Franquelim HG; Carvalho FA; Castanho MA; Santos NC. "Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria.". Nanomedicine : nanotechnology, biology, and medicine 10 (2014): 543-551. http://europepmc.org/abstract/med/24262996.
    Publicado • 10.1016/j.nano.2013.11.002
  19. Domingues MM; Bianconi ML; Barbosa LR; Santiago PS; Tabak M; Castanho MA; Itri R; Santos NC. "rBPI21 interacts with negative membranes endothermically promoting the formation of rigid multilamellar structures.". Biochimica et Biophysica Acta 1828 (2013): 2419-2427. http://europepmc.org/abstract/med/23792068.
    Publicado • 10.1016/j.bbamem.2013.06.009
  20. Domingues MM; Santos NC; Castanho MA. "Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies.". Current protein & peptide science 13 (2012): 611-619. http://europepmc.org/abstract/med/23116442.
    Publicado • 10.2174/138920312804142101
  21. Domingues MM; Inácio RG; Raimundo JM; Martins M; Castanho MA; Santos NC. "Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems.". Biopolymers 98 (2012): 338-344. http://europepmc.org/abstract/med/23193598.
    Publicado • 10.1002/bip.22095
  22. Ribeiro MM; Domingues MM; Freire JM; Santos NC; Castanho MA. "Translocating the blood-brain barrier using electrostatics.". Frontiers in Cellular Neuroscience 6 (2012): 44. http://europepmc.org/abstract/med/23087614.
    Publicado • 10.3389/fncel.2012.00044
  23. Ribeiro MM; Pinto AR; Domingues MM; Serrano I; Heras M; Bardaji ER; Tavares I; Castanho MA. "Chemical conjugation of the neuropeptide kyotorphin and ibuprofen enhances brain targeting and analgesia.". Molecular pharmaceutics 8 (2011): 1929-1940. http://europepmc.org/abstract/med/21830793.
    Publicado • 10.1021/mp2003016
  24. Freire JM; Domingues MM; Matos J; Melo MN; Veiga AS; Santos NC; Castanho MA. "Using zeta-potential measurements to quantify peptide partition to lipid membranes.". European biophysics journal : EBJ 40 (2011): 481-487. http://europepmc.org/abstract/med/21229352.
    Publicado • 10.1007/s00249-010-0661-4
  25. Santiago PS; Carvalho JW; Domingues MM; Santos NC; Tabak M. "Thermal stability of extracellular hemoglobin of Glossoscolex paulistus: determination of activation parameters by optical spectroscopic and differential scanning calorimetric studies.". Biophysical chemistry 152 (2010): 128-138. http://europepmc.org/abstract/med/20875698.
    Publicado • 10.1016/j.bpc.2010.08.010
  26. Santiago PS; Carvalho FA; Domingues MM; Carvalho JW; Santos NC; Tabak M. "Isoelectric point determination for Glossoscolex paulistus extracellular hemoglobin: oligomeric stability in acidic pH and relevance to protein-surfactant interactions.". Langmuir : the ACS journal of surfaces and colloids 26 (2010): 9794-9801. http://europepmc.org/abstract/med/20423061.
    Publicado • 10.1021/la100060p
  27. Domingues MM; Lopes SC; Santos NC; Quintas A; Castanho MA. "Fold-unfold transitions in the selectivity and mechanism of action of the N-terminal fragment of the bactericidal/permeability-increasing protein (rBPI(21)).". Biophysical journal 96 (2009): 987-996. http://europepmc.org/abstract/med/19186136.
    10.1016/j.bpj.2008.10.044
  28. Domingues MM; Castanho MA; Santos NC. "rBPI(21) promotes lipopolysaccharide aggregation and exerts its antimicrobial effects by (hemi)fusion of PG-containing membranes.". PLoS ONE 4 (2009): e8385. http://europepmc.org/abstract/med/20027298.
    Publicado • 10.1371/journal.pone.0008385
  29. Domingues MM; Santiago PS; Castanho MA; Santos NC. "What can light scattering spectroscopy do for membrane-active peptide studies?". Journal of peptide science : an official publication of the European Peptide Society 14 (2008): 394-400. http://europepmc.org/abstract/med/18189339.
    Publicado • 10.1002/psc.1007
  30. Santiago PS; Moura F; Moreira LM; Domingues MM; Santos NC; Tabak M. "Dynamic light scattering and optical absorption spectroscopy study of pH and temperature stabilities of the extracellular hemoglobin of Glossoscolex paulistus.". Biophysical Journal 94 (2008): 2228-2240. http://europepmc.org/abstract/med/18065453.
    Publicado • 10.1529/biophysj.107.116780
Capítulo de livro
  1. Domingues, Marco M.; Felício, Mário R.; Gonçalves, Sónia. "Antimicrobial Peptides: Effect on Bacterial Cells". In Methods in Molecular Biology, 233-242. Springer New York, 2018.
    Publicado • 10.1007/978-1-4939-8894-5_13
  2. Domingues, Marco; Santos, Nuno C.. "Laser-light scattering approach to peptide–membrane interaction.". In Membrane-active peptides: methods and results on structure and function, 145-168. IUL Biotechnology, 2010.
    Publicado
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2019/10/02 Role of the fibrin aC region in mechanical strength, resistance to fibrinolysis and the formation of a stable whole blood clot. European Congress on Thrombosis and Haemostasis (ECTH)
(Glasgow, Reino Unido)
2019/07/06 The fibrin aC region is critical for mechanical strength, resistance to fibrinolysis and the formation of a stable whole blood clot. International Society on Thrombosis and Haemostasis
(Melbourne, Austrália)
2019/04/17 Quantitative Imaging in Biological Applications Requimte AFM Training Workshop
(Porto, Portugal)
2017/07/20 Liposomes as drug delivery system of fibrinolytic agents to blood clots Colégio de Química
Universidade de Lisboa (Lisboa, Portugal)
2016/06/20 Evidence for a second factor XIII cross-linking site for alpha2-AP in fibrin. XXIVth International Fibrinogen Workshop
(Skukuza, África do Sul)
2016/06/20 Impact of gamma’ fibrinogen on the interaction with erythrocytes. XXIVth International Fibrinogen Workshop
(Skukuza, África do Sul)
2016/02/10 Conformational changes governing dengue virus capsid protein disordered N-terminal region and its inhibition by pep14-23. EPI 2016 – 15th Iberian Peptide Meeting
(Porto, Portugal)
2015/10/06 Conformational changes governing dengue virus capsid protein disordered N-terminal region and its inhibition by pep14-23. 1st NGP-NET Symposium on Non-Globular Proteins
(Porto, Portugal)
2015/06/20 Thrombin and fibrinogen ¿’ regulate protofibril packing within the fibrin fiber leading to changes in clot structure and strength. International Society on Thrombosis and Haemostasis
(Toronto, Canadá)
2014/10/07 Thrombin regulates protofibril packing within fibrin fibers leading to changes in clot stiffness. BSHT/UKHCDO/UK Platelet Group Joint Meeting
(Edinburgh, Reino Unido)
2014/09/10 Protofibril packing within fibrin fibers in thrombin concentraion-dependent manner. Early Career Research Conference in Cardiovascular and Diabetes Research
(Leeds, Reino Unido)
2014/08/18 Protofibril packing within fibrin fibers in thrombin concentraion-dependent manner. 4th Bio-Nano Summer School
(Hirschegg, Áustria)
2008/05/24 rBPI21 aggregates lipopolysaccharide and induces both (hemi)fusion and leakage of negatively charged membranes. XII Encuentro Peptídico Ibérico
(Lisboa, Portugal)
2008/05/24 Mechanism of the binding of rBPI21 with LPS aggregates and membrane model systems. 11th Naples workshop on bioactive peptides – New frontiers in the search of bioactive molecules: from peptide to drugs
(Naples, Itália)
2008/03/06 Elucidating the mechanisms of the interaction of the antimicrobial rBPI21 with lipopolysaccharide aggregates and membrane model systems. XI Encuentro Peptídico Ibérico
(Santiago, Espanha)

Participação em evento

Descrição da atividade
Tipo de evento 		Nome do evento
Instituição / Organização
2019/05/15 - 2019/05/17 Session Chair
Encontro
 XIII CAML (Lisbon Academic Medical Centre) PhD meeting
Instituto de Medicina Molecular, Portugal
2018/12/13 - 2018/12/14 Session Chair
Oficina (workshop)
 Session II- New thechnologies in drug development in Innovative approaches in targeted therapies: Brain, Infections and Cancer
Instituto de Medicina Molecular, Portugal

Arbitragem científica em conferência

Nome da conferência Local da conferência
2018/07/06 - 2018/07/06 Biophysics Party ITQB-NOVA

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2021/10/01 - 2022/01/31 Associate Invited lecturer at Faculty of Medicine of University of Lisbon. Biochemistry Module II.I of 1st year Medical Degree Medicine (Bachelor) Faculdade de Medicina da Universidade de Lisboa, Portugal
2020/10/01 - 2021/01/31 Associate Invited lecturer at Faculty of Medicine of University of Lisbon. Biochemistry Module II.I of 1st year Medical Degree Medicina (Licenciatura) Universidade de Lisboa Faculdade de Medicina, Portugal
2020/03/06 - 2020/05/22 Associate Invited lecturer at Faculty of Medicine of University of Lisbon. Biochemistry of 1st year Nutitional Sciences Degree Ciências da Nutrição (Licenciatura) Universidade de Lisboa Faculdade de Medicina, Portugal
2019/09/30 - 2020/01/30 Associate Invited lecturer at Faculty of Medicine of University of Lisbon. Biochemistry Module II.I of 1st year Medical Degree Medicina (Mestrado integrado) Universidade de Lisboa Faculdade de Medicina, Portugal
2019/01/09 - 2019/01/09 Invited lecturer for the FCT PhD programme in Medicines and Pharmaceutical Innovation (i3DU). (Doutoramento) Universidade de Lisboa Faculdade de Farmácia, Portugal
2018/01/29 - 2018/01/30 Invited lecturer for the Lisbon BioMed 2018 PhD Program (Doutoramento) Instituto de Medicina Molecular, Portugal
2017/01/30 - 2017/01/31 Invited lecturer for the Lisbon BioMed 2017 PhD Program. (Doutoramento) Instituto de Medicina Molecular, Portugal

Membro de associação

Nome da associação Tipo de participação
2017/01/31 - 2018/01/31 iMM PostDoctoral Association Organization of workshops, iMM PostDoc community retreat and iMM PostDoc Day. I organize two workshops, one related to IP and Tech Transfer (by Dr. Marta Ribeiro, UCL, UK) and other focused on scientific Illustration (Dr. Gil Costa, Champalimaud Institute, Portugal). Regarding the retreat, it was organized a job fair focusing on jobs outside academia.

Membro de comissão

Descrição da atividade
Tipo de participação 		Instituição / Organização
2018/03/31 - 2019/03/31 Evaluation of the PhD project progress of iMM PhD students: Susana Dias, Marcin Makowski and Ana Cruz
Membro
 Instituto de Medicina Molecular, Portugal

Outro júri / avaliação

Descrição da atividade Instituição / Organização
2018/05/24 - 2018/05/26 Member of Poster Evaluation in XI CAML (Lisbon Academic Medical Centre) PhD meeting (2017)
2018/05/23 - 2018/05/25 Member of Oral Evaluation Panel - XII CAML (Lisbon Academic Medical Centre) PhD meeting (2018) Instituto de Medicina Molecular, Portugal
2017/11/09 - 2017/11/09 Opponet evaluator of Master Thesis - Dr. Ana Filipa Daniel da Cruz (2017) – “Study of antimicrobial peptides’ activity against bacterial biofilms” Universidade de Lisboa Faculdade de Ciências, Portugal
2017/05/24 - 2017/05/26 Member of Poster Evaluation Panel - XI CAML (Lisbon Academic Medical Centre) PhD meeting (2017) Instituto de Medicina Molecular, Portugal

Tutoria

Tópico Nome do aluno
2020/11/01 - 2021/08/31 Development of a target therapy for fibrin clot breakdown Catarina Andreia Baptista Matos
2020/05/02 - 2020/09/03 Is nanothrombolysis the future? A comprehensive view of Acute Ischemic Stroke: Haemostasis, Thrombolytics and a new approach through nanomedicine. Diogo José Carvalho Vaz Pinto
2020/01/18 - 2020/07/01 Effect of knob B mimetic peptides on fibrin polymerization by light scattering approach Inês da Silva Oliveira
2019/07/01 - 2019/07/31 Summer Internship Ioanna Myrto Andrikaki
2017/10/01 - 2018/03/01 Liposome encapsulation of thrombolitic agents Francisco Correia dos Santos
2017/12/01 - 2018/02/19 Characterization and development of liposome encapsulating thrombolitic agent Mariana Morais Teixeira
2017/01/26 - 2017/06/19 Liposome characterization for further thrombolitic encapsulation in clot structure Diogo André Sequeira
Distinções

Prémio

2015 ISTH Young Investigators Grant
International Society on Thrombosis and Haemostasis Inc, Estados Unidos
2009 Stimulus to Investigation Award
Fundação Calouste Gulbenkian, Portugal