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Hugo M. Botelho is a Junior Researcher at the Biosystems & Integrative Sciences Institute (BioISI) of the Faculty of Sciences University of Lisbon (FCUL) and Manager of BioISI's High-Throughput Screening (HTS) Facility. Scientist with a strong expertise in microscopy, cell biology, bioimage analysis, drug discovery and protein folding in vivo and in vitro. Obtained his PhD in Biochemistry (specialty: biophysics) in 2010 under the supervision of Cláudio M. Gomes (ITQB/UNL) for studies on protein folding and aggregation. In 2012 joined Margarida Amaral's lab at FCUL to research novel therapeutics for Cystic Fibrosis (CF). For 1.5 years was trained on high-throughput microscopy, RNA interference screening and membrane trafficking at EMBL Heidelberg. Since 2014 he has been employing siRNA-based screens to characterize novel therapeutic CF target genes. As principal investigator he has coordinated research projects focusing on the identification and characterization of novel molecules with therapeutic relevance for CF using natural compound sources and HTS methodologies. Since 2014 he has established a HTS Facility at BioISI and now serves as its manager, which includes supervision of 1 research technician. Each year the facility provides 5000 hours of equipment access time to about 40 users regarding sample preparation, imaging, data analysis and screening assay services. He is also actively engaged in developing and deploying automated image analysis algorithms for high content microscopy, software development and data science approaches to biological data. Has authored 29 original peer reviewed publications, 4 book chapters, supervised students and post-docs and been involved in several scientific research projects, both as team member and principal investigator. As manager of the HTS Facility, represents BioISI and FCUL at the Portuguese Platform of BioImaging (PPBI), PT-OPENSCREEN, Euro-BioImaging ERIC, EU-OPENSCREEN, the Lisbon Area BioImaging Group, among others. He is a regular trainer and speaker at microscopy and CF meetings and courses. h-index 14 (Scopus, May 2023)
Identificação

Identificação pessoal

Nome completo
Hugo Miguel Raposo Correia Botelho
Género
Masculino

Nomes de citação

  • Botelho, Hugo M

Identificadores de autor

Ciência ID
E218-E579-24C1
ORCID iD
0000-0002-4208-1086
Google Scholar ID
nXftyYwAAAAJ&hl
Researcher Id
B-3092-2008
Scopus Author Id
15030102200

Endereços de correio eletrónico

  • hmbotelho@ciencias.ulisboa.pt (Profissional)

Telefones

Telefone
  • 217500659 (Profissional)

Moradas

  • BioISI - Biosystems & Integrative Sciences Institute. Campo Grande, C8, 1749-016, Lisboa, Lisboa, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências Exatas - Ciências da Computação e da Informação
  • Ciências Médicas e da Saúde - Biotecnologia Médica
  • Ciências Naturais - Ciências Biológicas - Biofísica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Francês Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1)
Espanhol; Castelhano Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1)
Português (Idioma materno)
Formação
Grau Classificação
2010/11/22
Concluído
Biochemistry (Doutoramento)
Especialização em Biophysics
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
"Metal Ions and Protein Folding - Conformational and Functional Interplay" (TESE/DISSERTAÇÃO)
Approved by unanimity
2006/07/14
Concluído
Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Pesquisa, identificação e caracterização de proteínas hiperestáveis no proteoma solúvel da Archaea hipertermofílica Sulfurisphaera sp." (TESE/DISSERTAÇÃO)
18
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2019/07/01 - Atual Investigador Contratado (Investigação) Universidade de Lisboa Faculdade de Ciências, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2015/05/01 - 2019/06/30 Pós-doutorado (Investigação) Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2012/04/15 - 2019/06/30 Pós-doutorado (Investigação) Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2012/07/16 - 2013/12/31 Investigador visitante (Investigação) European Molecular Biology Laboratory, Alemanha
2012 - 2013 Investigador (Investigação) Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2011/09/01 - 2012/04/14 Pós-doutorado (Investigação) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2011/07/03 - 2011/10/03 Pós-doutorado (Investigação) Universitätsklinikum Freiburg Neurologische Universitätsklinik, Alemanha
2010/11/23 - 2011/06/30 Pós-doutorado (Investigação) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2011 - 2011 Investigador (Investigação) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/01/25 - 2010/02/07 Investigador visitante (Investigação) Umea Universitet, Suécia
2005/09/05 - 2006/07/14 Estagiário de Investigação (Investigação) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2006/10/01 - 2010/11/22 PhD Student Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Projetos

Bolsa

Designação Financiadores
2016/05/01 - 2018/01/01 The identification of new natural compounds of high therapeutic potential for Cystic Fibrosis by high-throughput microscopy screens
BioISI Post-Doc
Orientador
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas
Concluído

Projeto

Designação Financiadores
2024/01/01 - Atual ORGESTRA - Organoid technologies for disease modeling, drug discovery and development for rare diseases
Horizon Europe, Bélgica
Em curso
2022/02/24 - Atual ISIDORe - Integrated Services for Infectious Diseases Outbreak Research
Investigador
Universidade de Lisboa Faculdade de Ciências, Portugal
European Union
Em curso
2024/02 - 2024/09 Biocompatible Coatings with Natural-Based Nano-Agents for Biomedical Applications
BioISI Projects 2024
Co-Investigador Responsável (Co-IR)
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2023/01/01 - 2024/06/30 NewKinCF - Unraveling the mechanism of action of a novel kinase regulator of F508del-CFTR traffic and activity
2022.03453.PTDC
Investigador responsável
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2022/05 - 2023/04 Mitochondrial network in Multiple Acyl-CoA Dehydrogenase Deficiency: construction of a high-content bioimage analysis workflow
BioISI Projects 2022
Investigador responsável
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2017/06/01 - 2021/10/31 PPBI - Portuguese Platform of BioImage
Investigador
Universidade de Lisboa Faculdade de Ciências, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2018/10/01 - 2021/09/30 Identificação de Novos Fármacos para Fibrose Quística
PTDC/MED-QUI/28800/2017
Fundação para a Ciência e a Tecnologia
Em curso
2020/02/04 - 2021/03/31 VALHealth - Valorisation of Algae for Health: Bioactive Compounds from Marine Bioresources by Membrane Technology
BioISI Projects 2020
Investigador responsável
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas
Concluído
2018 - 2021 AbrogateBM: Detect, prevent, erradicate: a multi-step strategy to abrogate brain metastasization of breast cancer
Consultant
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Associação para a Inovação e Desenvolvimento da FCT
Concluído
2015/09/01 - 2020/05/31 CORBEL - Coordinated Research Infrastructures Building Enduring Life-science Services
VIP User
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
European Union
Concluído
2019/01/01 - 2019/12/31 Instituto de Biosistemas & Ciências Integrativas
UID/Multi/04046/2019
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Universidade do Minho, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal

FCiênciasID Associação para a Investigação e Desenvolvimento de Ciências, Portugal

Universidade de Lisboa Faculdade de Ciências, Portugal

Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2016 - 2019 Novel CFTR Traffic Factors Related with Epithelial Cell Differentiation: Potential Therapeutic Targets for Cystic Fibrosis
Investigador
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2018/02/01 - 2018/12/31 Deconvolution of dual CFTR/ANO1 Modulators from Portuguese natural products - A new class of drugs for CF therapy
BioISI Projects 2018
Investigador responsável
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas
Concluído
2016 - 2018 MIMED - Mining the molecular metric space for drug design
Investigador
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2017/01/07 - 2017/12/30 A new class of drugs for CF therapy - Dual CFTR/ANO1 Modulators from Portuguese natural products
BioISI Projects 2017
Investigador responsável
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas
Concluído
2014 - 2017 Innovative non-CFTR approaches to cystic fibrosis therapy
Strategic Research Centre Grant
Investigador
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Cystic Fibrosis Trust
Concluído
2016/01/05 - 2016/12/16 Natural compounds as a source of novel drug leads for Cystic Fibrosis
BioISI Projects 2016
Investigador responsável
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas
Concluído
2011 - 2013 Identification of novel targets rescuing of F508del-CFTR traffic: Mechanism of action
Investigador
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Cystic Fibrosis Foundation
Concluído
2011/01/01 - 2012/12/31 Strategic Project - LA 4 - 2011-2012
PEst-OE/EQB/LA0004/2011
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal

Fundação Calouste Gulbenkian, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

Universidade NOVA de Lisboa NOVA Medical School, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2012/02/01 - 2012/04/15 Mechanisms of SOD1 toxic aggregation in neurodegenerative processes
Investigador
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2011 - 2012 Amyloidogenesis of the S100A8/A9 cytokine as an anti-proliferative mechanism in prostate cancer
Terry Fox
Investigador
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Liga Portuguesa Contra o Cancro
Concluído
2008/01/02 - 2011/12/31 Towards the understanding of the energetic and structural interplay between metal sites and protein folding
Investigador
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2007/07/01 - 2011/06/30 Functional analysis and origins of the pathophysiology of mutations within genes of the fatty acid oxidation pathway: implications in the multiple acyl-CoA dehydrogenase deficiency disorder
Investigador
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em revista
  1. Mariana Coelho; Ana Patrícia Duarte; Sofia Pinto; Hugo M. Botelho; Catarina Pinto Reis; Maria Luísa Serralheiro; Rita Pacheco. "Edible Seaweeds Extracts: Characterization and Functional Properties for Health Conditions". Antioxidants 12 3 (2023): 684. https://doi.org/10.3390/antiox12030684.
    Publicado • 10.3390/antiox12030684
  2. Margarida C Quaresma; Hugo M Botelho; Ines Pankonien; Cláudia S Rodrigues; Madalena C Pinto; Pau R Costa; Aires Duarte; Margarida D Amaral. "Exploring YAP1-centered networks linking dysfunctional CFTR to epithelial–mesenchymal transition". Life Science Alliance (2022): https://doi.org/10.26508/lsa.202101326.
    10.26508/lsa.202101326
  3. Joana Godinho-Pereira; Margarida Dionísio Lopes; Ana Rita Garcia; Hugo M. Botelho; Rui Malhó; Inês Figueira; Maria Alexandra Brito. "A Drug Screening Reveals Minocycline Hydrochloride as a Therapeutic Option to Prevent Breast Cancer Cells Extravasation across the Blood–Brain Barrier". Biomedicines (2022): https://doi.org/10.3390/biomedicines10081988.
    10.3390/biomedicines10081988
  4. João F. Ferreira; Iris A. L. Silva; Hugo M. Botelho; Margarida D. Amaral; Carlos M. Farinha. "Absence of EPAC1 Signaling to Stabilize CFTR in Intestinal Organoids". Cells (2022): https://doi.org/10.3390/cells11152295.
    10.3390/cells11152295
  5. Célia Fernandes; Elisa Palma; Francisco Silva; Ana Belchior; Catarina I. G. Pinto; Joana F. Guerreiro; Hugo M. Botelho; et al. "Searching for a Paradigm Shift in Auger-Electron Cancer Therapy with Tumor-Specific Radiopeptides Targeting the Mitochondria and/or the Cell Nucleus". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms23137238.
    10.3390/ijms23137238
  6. Pinto, Madalena C.; Botelho, Hugo M; Silva, Iris AL; Violeta Railean; Beate Neumann; Rainer Pepperkok; Rainer Schreiber; Karl Kunzelmann; Amaral, Margarida. "Systems Approaches to Unravel Molecular Function: High-content siRNA Screen Identifies TMEM16A Traffic Regulators as Potential Drug Targets for Cystic Fibrosis". Journal of Molecular Biology 434 5 (2022): 167436-167436. http://dx.doi.org/10.1016/j.jmb.2021.167436.
    Publicado • 10.1016/j.jmb.2021.167436
  7. Sang Hyun Lim; Jamie Snider; Liron Birimberg-Schwartz; Wan Ip; Joana C Serralha; Botelho, Hugo M; Miquéias Lopes-Pacheco; et al. "CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system". Molecular Systems Biology 18 2 (2022): https://doi.org/10.15252/msb.202110629.
    10.15252/msb.202110629
  8. Marne C Hagemeijer; Annelotte M Vonk; Nikhil T Awatade; Iris A L Silva; Christian Tischer; Volker Hilsenstein; Jeffrey M Beekman; et al. Autor correspondente: Hugo M Botelho. "An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay". Bioinformatics (2020): https://doi.org/10.1093/bioinformatics/btaa1073.
    10.1093/bioinformatics/btaa1073
  9. Silva IAL; Doušová T; Ramalho S; Centeio R; Clarke LA; Railean V; Botelho, Hugo M; et al. "Organoids as a personalized medicine tool for ultra-rare mutations in cystic fibrosis: The case of S955P and 1717-2A>G.". Biochimica et biophysica acta. Molecular basis of disease 1866 (2020): 165905. http://europepmc.org/abstract/med/32730979.
    Publicado • 10.1016/j.bbadis.2020.165905
  10. Inna Uliyakina; Botelho, Hugo M; Ana C. da Paula; Sara Afonso; Miguel J. Lobo; Verónica Felício; Carlos M. Farinha; Margarida D. Amaral. "Full Rescue of F508del-CFTR Processing and Function by CFTR Modulators Can Be Achieved by Removal of Two Regulatory Regions". International Journal of Molecular Sciences 21 12 (2020): 4524-4524. https://doi.org/10.3390/ijms21124524.
    Publicado • 10.3390/ijms21124524
  11. Amaral, Margarida D.; Hutt, Darren M; Tomati, Valeria; Botelho, Hugo M; Pedemonte, Nicoletta. "CFTR processing, trafficking and interactions". Journal of Cystic Fibrosis 19 (2020): S33-S36. http://dx.doi.org/10.1016/j.jcf.2019.10.017.
    Publicado • 10.1016/j.jcf.2019.10.017
  12. João D. Santos; Sara Canato; Ana S. Carvalho; Botelho, Hugo M; Kerman Aloria; MD Amaral; Rune Matthiesen; Andre O. Falcao; Carlos Farinha. "Folding Status Is Determinant over Traffic-Competence in Defining CFTR Interactors in the Endoplasmic Reticulum". Cells 8 4 (2019): 353. https://www.mdpi.com/2073-4409/8/4/353.
    Publicado • 10.3390/cells8040353
  13. Awatade, Nikhil T.; Ramalho, Sofia; Silva, Iris A.L.; Felício, Verónica; Botelho, Hugo M; de Poel, Eyleen; Vonk, Annelotte; et al. "R560S: A class II CFTR mutation that is not rescued by current modulators". Journal of Cystic Fibrosis 18 2 (2019): 182-189. http://dx.doi.org/10.1016/j.jcf.2018.07.001.
    Publicado • 10.1016/j.jcf.2018.07.001
  14. Palma, Elisa; Botelho, Hugo M; Morais, Goreti Ribeiro; Rodrigues, Inês; Santos, Isabel Cordeiro; Campello, Maria Paula Cabral; Raposinho, Paula; et al. "Unravelling the antitumoral potential of novel bis(thiosemicarbazonato) Zn(II) complexes: structural and cellular studies". JBIC Journal of Biological Inorganic Chemistry 24 1 (2018): 71-89. http://dx.doi.org/10.1007/s00775-018-1629-6.
    Publicado • 10.1007/s00775-018-1629-6
  15. Joana S. Cristóvão; Vanessa K. Morris; Isabel Cardoso; Sónia S. Leal; Javier Martínez; Botelho, Hugo M; Christoph Göbl; et al. "The neuronal S100B protein is a calcium-tuned suppressor of amyloid-ß aggregation". Science Advances 4 6 (2018): eaaq1702-eaaq1702. https://doi.org/10.1126/sciadv.aaq1702.
    Publicado • 10.1126/sciadv.aaq1702
  16. Lérias, Joana R.; Pinto, Madalena C.; Botelho, Hugo M; Awatade, Nikhil T.; Quaresma, Margarida C.; Silva, Iris A.L.; Wanitchakool, Podchanart; et al. "A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1865 2 (2018): 421-431. http://dx.doi.org/10.1016/j.bbamcr.2017.11.009.
    Publicado • 10.1016/j.bbamcr.2017.11.009
  17. Botelho, Hugo; Amaral, Margarida D.; Farinha, Carlos M.; Matos, Paulo; Botelho, Hugo M.. "Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).". Methods in molecular biology (Clifton, N.J.) (2016): 105-126. http://europepmc.org/abstract/med/27665554.
    Publicado • 10.1007/978-1-4939-3804-9_7
  18. Igreja, Susana; Clarke, Luka A.; Botelho, Hugo M; Marques, Luís; Amaral, Margarida D.. "Correction of a Cystic Fibrosis Splicing Mutation by Antisense Oligonucleotides". Human Mutation 37 2 (2015): 209-215. http://dx.doi.org/10.1002/humu.22931.
    Publicado • 10.1002/humu.22931
  19. Clarke, Luka A.; Botelho, Hugo M; Sousa, Lisete; Falcao, Andre O.; Amaral, Margarida D.. "Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators". Genomics 106 5 (2015): 268-277. http://dx.doi.org/10.1016/j.ygeno.2015.07.005.
    Publicado • 10.1016/j.ygeno.2015.07.005
  20. Botelho, Hugo M; Uliyakina, Inna; Awatade, Nikhil T.; Proença, Maria C.; Tischer, Christian; Sirianant, Lalida; Kunzelmann, Karl; Pepperkok, Rainer; Amaral, Margarida D.. "Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery". Scientific Reports 5 1 (2015): http://dx.doi.org/10.1038/srep09038.
    Publicado • 10.1038/srep09038
  21. Igreja, S.; Clarke, L. A.; Botelho, H. M.; Marques, L.; Amaral, M. D.. "Correction of a cystic fibrosis splicing mutation by antisense oligonucleotides". Human Mutation (2015):
  22. Carvalho, Sofia B.; Botelho, Hugo M; Leal, Sónia S.; Cardoso, Isabel; Fritz, Günter; Gomes, Cláudio M.. "Intrinsically Disordered and Aggregation Prone Regions Underlie ß-Aggregation in S100 Proteins". PLoS ONE 8 10 (2013): e76629. http://dx.doi.org/10.1371/journal.pone.0076629.
    Publicado • 10.1371/journal.pone.0076629
  23. Botelho, Hugo M; Dahimène, S.; Uliyakina, I.; Neumann, B.; Tischer, C.; Pepperkok, R.; Amaral, M.D.. "WS20.6 High throughput screening as a source for novel cystic fibrosis therapeutic targets". Journal of Cystic Fibrosis 12 (2013): S42. http://dx.doi.org/10.1016/s1569-1993(13)60128-9.
    10.1016/s1569-1993(13)60128-9
  24. Dahimène, S.; Romão, A.; Botelho, Hugo M; Palma, M.; Correia, A.R.; Farinha, C.M.; King-Underwood, J.; et al. "WS21.9 Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays". Journal of Cystic Fibrosis 12 (2013): S45. http://dx.doi.org/10.1016/s1569-1993(13)60137-x.
    Publicado • 10.1016/s1569-1993(13)60137-x
  25. Sá-Moura, Bebiana; Simões, Ana Marisa; Fraga, Joana; Fernandes, Humberto; Abreu, Isabel A.; Botelho, Hugo M; Gomes, Cláudio M.; et al. "Biochemical and biophysical characterization of recombinant yeast proteasome maturation factor Ump1". Computational and Structural Biotechnology Journal 7 8 (2013): e201304006. http://dx.doi.org/10.5936/csbj.201304006.
    Publicado • 10.5936/csbj.201304006
  26. Veith, A.; Botelho, Hugo M; Kindinger, F.; Gomes, C.M.; Kletzin, A.. "The sulfur oxygenase reductase from the mesophilic bacterium Halothiobacillus neapolitanus is a highly active thermozyme". Journal of Bacteriology 194 3 (2012): 677-685. http://www.scopus.com/inward/record.url?eid=2-s2.0-84855915891&partnerID=MN8TOARS.
    Publicado • 10.1128/JB.06531-11
  27. Leal, S.S.; Botelho, Hugo M; Gomes, C.M.. "Metal ions as modulators of protein conformation and misfolding in neurodegeneration". Coordination Chemistry Reviews 256 19-20 (2012): 2253-2270. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864548981&partnerID=MN8TOARS.
    Publicado • 10.1016/j.ccr.2012.04.004
  28. Botelho, Hugo M; Leal, S.S.; Cardoso, I.; Yanamandra, K.; Morozova-Roche, L.A.; Fritz, G.; Gomes, C.M.. "S100A6 amyloid fibril formation is calcium-modulated and enhances superoxide dismutase-1 (SOD1) aggregation". Journal of Biological Chemistry 287 50 (2012): 42233-42242. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871281215&partnerID=MN8TOARS.
    Publicado • 10.1074/jbc.M112.396416
  29. Botelho, Hugo M; Gomes, C.M.. "Structural reorganization renders enhanced metalloprotein stability". Chemical Communications 47 39 (2011): 11149-11151. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053318341&partnerID=MN8TOARS.
    Publicado • 10.1039/c1cc13354c
  30. Fritz, G.; Botelho, Hugo M; Morozova-Roche, L.A.; Gomes, C.M.. "Natural and amyloid self-assembly of S100 proteins: Structural basis of functional diversity". FEBS Journal 277 22 (2010): 4578-4590. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049277867&partnerID=MN8TOARS.
    Publicado • 10.1111/j.1742-4658.2010.07887.x
  31. Botelho, Hugo M; Leal, S.S.; Veith, A.; Prosinecki, V.; Bauer, C.; Fröhlich, R.; Kletzin, A.; Gomes, C.M.. "Role of a novel disulfide bridge within the all-beta fold of soluble Rieske proteins". Journal of Biological Inorganic Chemistry 15 2 (2010): 271-281. http://www.scopus.com/inward/record.url?eid=2-s2.0-77949270785&partnerID=MN8TOARS.
    Publicado • 10.1007/s00775-009-0596-3
  32. Botelho, Hugo M; Koch, M.; Fritz, G.; Gomes, C.M.. "Metal ions modulate the folding and stability of the tumor suppressor protein S100A2". FEBS Journal 276 6 (2009): 1776-1786. http://www.scopus.com/inward/record.url?eid=2-s2.0-61349168402&partnerID=MN8TOARS.
    Publicado • 10.1111/j.1742-4658.2009.06912.x
  33. Prosinecki, V.; Botelho, Hugo M; Francese, S.; Mastrobuoni, G.; Moneti, G.; Urich, T.; Kletzin, A.; Gomes, C.M.. "A proteomic approach toward the selection of proteins with enhanced intrinsic conformational stability". Journal of Proteome Research 5 10 (2006): 2720-2726. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750201140&partnerID=MN8TOARS.
    10.1021/pr0602491
Capítulo de livro
  1. Amaral, Margarida; Clarke, Luka A; Carlos M. Farinha; Botelho, Hugo. "Systems Biology and the New Omics". In Hodson and Geddes' Cystic Fibrosis, editado por Andrew Bush; Margarida D. Amaral; Jane C. Davies; Nicholas J. Simmonds; Jennifer L. Taylor-Cousar; Sarath C. Ranganathan, 96-110. Boca Raton, FL, Estados Unidos: Taylor & Francis. CRC Press, 2023.
    Publicado • 10.1201/9781003262763
  2. Carvalho, Sofia B.; Cardoso, Isabel; Botelho, Hugo M; Yanamandra, Kiran; Fritz, Günter; Gomes, Cláudio M.; Morozova-Roche, Ludmilla A.. "Structural Heterogeneity and Bioimaging of S100 Amyloid Assemblies". In Bio-nanoimaging, 197-212. Elsevier, 2014.
    Publicado • 10.1016/b978-0-12-394431-3.00018-3
  3. Botelho, Hugo M; Fritz, Günter; Gomes, Cláudio M.. "Analysis of S100 Oligomers and Amyloids". In Methods in Molecular Biology, 373-386. Humana Press, 2012.
    10.1007/978-1-61779-551-0_25
Poster em conferência
  1. Daniela Neugebauer; Mariana Caleiro; Vasco Cachatra; Moiteiro, Cristina; Amaral, Margarida; Botelho, Hugo M. "High content screening for the characterization of the GRK5-dependent p.Phe508del-CFTR rescue in cystic fibrosis". Trabalho apresentado em Spanish & Portuguese Advanced Optical Microscopy Meeting – SPAOM2023, 2023.
  2. Daniela Neugebauer; Vasco Cachatra; Moiteiro, Cristina; Amaral, Margarida; Botelho, Hugo M. "Identification of genes that restore p.Phe508del-CFTR traffic in cystic fibrosis models through GRK5 inhibition". Trabalho apresentado em Chem&BioChem – Postgraduate Students Meeting, DQB/FCUL, 2023.
  3. Marques, Rute; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Cyclin D1-CDK4/6 and PI3K signalling pathways as targets to combat metastatic triple negative and luminal A breast cancer". Trabalho apresentado em Jornadas Científicas 2023 Universidade de Lisboa, 2023.
  4. Marques, Rute; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Exploiting cyclin D1/CDK4/6 and/or PI3K pathways to fight metastatic triple negative and luminal A breast cancer". Trabalho apresentado em 14th iMed.ULisboa Postgraduate Students Meeting, 2023.
  5. Fernandes AA; Martinho, Paulo N.; Bento, Marcos; Botelho, Hugo. "Thermo- and Photo-responsive Spin Labile Fe(III) complexes". Trabalho apresentado em CQE Days 2023, 2023.
  6. Pinto S; DUARTE, Ana Patrícia; Botelho, Hugo; Gaspar, Maria Manuela; Ascenção L; Faísca P; Catarina Pinto Reis; Pacheco, Rita. "Albumin Nanoparticles of Natural Products: In Vitro and In Vivo Evaluation". Trabalho apresentado em 4th SPLC-CRS Young Scientists Meeting, 2023.
  7. Coelho M; DUARTE, Ana Patrícia; Botelho, Hugo; Catarina Pinto Reis; Maria Luísa Serralheiro; Pacheco, Rita. "Edible seaweeds extracts characterization and prospective health effects". Trabalho apresentado em 1st International Congress on Food, Nutrition & Public Health, 2022.
  8. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo; Malhó, Rui; Brito, Alexandra. "Detalhando as alterações fenotípicas e morfológicas subjacentes ao extravasamento das células de cancro da mama através da barreira hematoencefálica". Trabalho apresentado em 4º Encontro Nacional de Jovens Investigadores em Oncologia, 2022.
  9. M. A. Bento; Paulo Nuno Martinho; Botelho, Hugo M; Diogo, Hermínio P.; Liliana P. Ferreira; Vítor H. N. Rodrigues. Autor correspondente: M. A. Bento. "Fe(III) complexes as molecular magnetic actuators". Trabalho apresentado em MagnetismIn, 2022.
  10. Filipa S. Carvalho; Vukosava M Torres; Joana V. Ribeiro; Botelho, Hugo; Vilarinho, Laura; Cláudio M. Gomes; Henriques, Bárbara J. "Mitochondria phenotype and metabolomic signature in MADD patient-derived fibroblasts". Trabalho apresentado em SSIEM 2022 Annual Symposium, 2022.
  11. Marcos A. Bento; Botelho, Hugo; Ferreira, L.P.; Martinho, Paulo N.. "Molecular magnetic actuators based on Fe(III) complexes". Trabalho apresentado em EuChemS Chemistry Congress, 2022.
  12. Carvalho FS; Ribeiro JV; Botelho, Hugo; Vilarinho L; Gomes CM; Henriques, BJ. "Cellular studies to evaluate mitochondria function on MADD, a rare metabolic disorder". Trabalho apresentado em The Biochemistry Global Summit (25th IUBMB Congress, 46th FEBS Congress and 15th PABMB Congress), 2022.
  13. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Dissecting the extravasation of triple-negative breast cancer cells across the blood-brain barrier: the landscape for brain metastasis prevention". Trabalho apresentado em 13th iMed.ULisboa Postgraduate Students Meeting, 2022.
  14. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Molecular characterization and modulation of blood-brain barrier and breast cancer cells interaction: a step closer to brain metastasis prevention". Trabalho apresentado em EACR 2022 Congress ¿ Innovative Cancer Science: Translating Biology to Medicine, 2022.
  15. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Understanding the blood-brain barrier and breast cancer cells' interaction: a step closer to brain metastasis prevention". Trabalho apresentado em Brain Tumor Meeting 2022, 2022.
  16. Godinho-Pereira, Joana; Lopes, Margarida Dionísio; Ana Rita Garcia; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Tackling breast cancer brain metastasis: a drug screening for a preventive approach to boost blood-brain barrier properties". Trabalho apresentado em Brain Tumor Meeting 2022, 2022.
  17. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Emerging events in breast cancer cells extravasation through blood-brain barrier: the key for brain metastasis prevention". Trabalho apresentado em Encontro Ciência 2022, 2022.
  18. Ferreira, JF; Silva, Iris AL; Botelho, Hugo M; Amaral, Margarida; Farinha, CM. "Exploring the role of EPAC1 to stabilize CFTR in intestinal cells". Trabalho apresentado em 17th ECFS Basic Science Conference, 2022.
  19. Botelho, Hugo M; Lopes-Pacheco, Miquéias; Pinto, Madalena C.; Silva, Iris AL; Clarke, Luka; Cachatra, V; Neumann, B; et al. "New kinase therapeutic targets for Cystic Fibrosis from a global functional genomics screen". Trabalho apresentado em 17th ECFS Basic Science Conference, 2022.
  20. Botelho, Hugo M; Lopes-Pacheco, Miquéias; Pinto, Madalena C.; Silva, Iris AL; Clarke, Luka A; Cachatra, V; Neumann, B; et al. "New kinase therapeutic targets for Cystic Fibrosis identified through high-content functional genomics screens". Trabalho apresentado em 5ª Conferência Anual da redeSAÚDE, 2021.
  21. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Brito, MA. "Spatio-temporal and molecular characterization of breast cancer cells extravasation through blood-brain-barrier". Trabalho apresentado em iMed.ULisboa Meeting 2021, 2021.
  22. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Disclosing molecular alterations in the blood-brain barrier endothelium during interaction with breast cancer cells: a step forward brain metastasis prevention.". Trabalho apresentado em Encontro de Ciência 2021, 2021.
  23. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Brito, MA. "Unravelling the extravasation of breast cancer cells through blood-brain barrier endothelial cells". Trabalho apresentado em 27th Porto Cancer Meeting, 2021.
  24. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Unravelling the extravasation of breast cancer cells though blood-brain barrier endothelial cells.". Trabalho apresentado em Porto Cancer Meeting 2021, 2021.
  25. Dionisio Lopes, Margarida; Godinho-Pereira, Joana; Ana Rita Garcia; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "High-throughput screening of new and old drugs: a modulator to prevent breast cancer brain metastasis". Trabalho apresentado em Aims Meeting, 2021.
  26. Ana Rita Garcia; Joana Godinho-Pereira; Ines Figueira; KW Kim; Botelho, Hugo M; Rui Malhó; Maria Alexandra Brito. "Desvendando a interação entre as células de cancro da mama e as células endoteliais da barreira hematoencefálica ao longo do extravasamento". Trabalho apresentado em 2º Encontro Nacional de Jovens Investigadores em Oncologia, 2020.
  27. Garcia, R; Godinho-Pereira, J; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, R; Brito, MA. "Breast cancer cells extravasation into the brain: a dynamic trafficking across blood-brain barrier endothelium". Trabalho apresentado em 5th Annual Meeting of the Mind-Brain College of the University of Lisbon, 2019.
  28. Ramalho, AS; Vonk, AM; Silva, Iris AL; Botelho, Hugo M; Bor, R; van Mourik, P; Michel, S; et al. "High reproducibility of forskolin-induced swelling of intestinal organoids across three academic laboratories". Trabalho apresentado em 33rd Annual North American Cystic Fibrosis Conference, 2019.
  29. Garcia, R; Godinho-Pereira, J; Figueira, Inês; Cascão, R; Barata, J; Kim, KS; Marques, Luis; et al. "Interplay between breast cancer cells and blood-brain barrier endothelial cells along extravasation". Trabalho apresentado em 11th iMed.ULisboa Postgraduate Students Meeting and 4th i3DU Meeting, 2019.
  30. van Mourik, P; Vonk AM; Ramalho AS; Silva IAL; Botelho, Hugo; Bor R; Aguilera B; et al. "High reproducibility of forskolin-induced swelling of intestinal organoids across three academic laboratories". Trabalho apresentado em 42nd European Cystic Fibrosis Conference, 2019.
  31. Vonk, Annelotte M; Ramalho, Anabela S; Silva, Iris AL; Silva, Iris AL; Botelho, Hugo M; van Mourik, Peter; Michel, S; et al. "High reproducibility of forskolin-induced swelling of organoids across three academic laboratories.". Trabalho apresentado em 16th ECFS Basic Science Conference 2019, Dubrovnik, Croatia, 27-30 March 2019., 2019.
  32. Botelho, Hugo M; Halavatyi, A; Tischer, C. "shinyHTM: an open-source web application for the analysis of high throughput microscopy data". Trabalho apresentado em EMBL Conference: From Images to Knowledge with ImageJ & Friends (I2K), 2018.
  33. Astapenko, D; Botelho, Hugo M; Kolodkin, A; Westerhoff, HV; Amaral, Margarida. "Dynamic modelling of CFTR channel maturation in Cystic Fibrosis: what controls the channel concentration in the plasma membrane?". Trabalho apresentado em The 11th International Conference on Bioinformatics of Genome Regulation and Structure\Systems Biology, 2018.
  34. Quaresma, MC; Botelho, Hugo M; Pankonien, I; Pepperkok, R; Amaral, Margarida. "Role of CFTR in epithelial-mesenchymal transition (EMT) by functional genomics". Trabalho apresentado em Encontro Ciência 2018, 2018.
  35. Lopes-Pacheco, M; Botelho, Hugo M; Amaral, Margarida. "Novel F508del-CFTR traffic correctors among FDA-approved drugs and mechanism of action of RDR01752 corrector". Trabalho apresentado em EMBO Workshop: Proteostasis, 2017.
  36. Botelho, Hugo M; Lim, SH; Tischer, C; Pepperkok, R; Stagljar, I; Amaral, Margarida. "Validation and classification of genes releasing misfolded F508del-CFTR from the endoplasmic reticulum". Trabalho apresentado em EMBO Workshop: Proteostasis, 2017.
  37. Awatade, NT; Felício, V; Ramalho, S; Botelho, Hugo M; Silva, Iris AL; de Poel, E; Vonk, AM; et al. "Functional and biochemical assessment of correctors and potentiators on the rare Cystic Fibrosis-causing R560S mutation". Trabalho apresentado em 31st Annual North American Cystic Fibrosis Conference, 2017.
  38. Pinto, MC; Lérias, JR; Botelho, Hugo M; Awatade, NT; Pepperkok, R; Kunzelmann, K; Amaral, Margarida. "Anoctamin 1 – Identification of novel regulators and their significance to Cystic Fibrosis". Trabalho apresentado em 31st Annual North American Cystic Fibrosis Conference, 2017.
  39. Cristóvão, JS; Morris, V; Cardoso, Isabel; Leal, SS; Botelho, Hugo M; Göbl, C; Kierdorf, K; et al. "The neuronal S100B cytokine targeting amyloid-ß aggregation in Alzheimer’s Disease". Trabalho apresentado em 42nd FEBS Congress, 2017.
  40. Canato, S; Carvalho, AS; Botelho, Hugo M; Aloria, K; Matthiesen, Rune; Amaral, Margarida; Falcão, AO; Farinha, CM. "Modulation of protein traffic networks to rescue F508del-CFTR from the endoplasmic reticulum". Trabalho apresentado em 40th European Cystic Fibrosis Conference, 2017.
  41. Baptista, C; Botelho, Hugo M; Tenreiro, RP; Awatade, NT; Amaral, Margarida; Vieira, H. "From Portuguese natural heritage to Cystic Fibrosis therapies – a story of nature and technology for pharmaceutical applications". Trabalho apresentado em 3rd Annual Conference Rede Mar, 2017.
  42. Cristóvão, J; Morris, V; Cardoso, Isabel; Leal, SS; Botelho, Hugo M; Göbl, C; Kierdorf, K; et al. "Role of the S100B pro-inflammatory cytokine implicated in aging and Alzheimer’s Disease as a calcium-regulated chaperone acting on amyloid-ß aggregation". Trabalho apresentado em 4th Annual Conference Rede Saúde, 2017.
  43. Quaresma, MC; Botelho, Hugo M; Amaral, Margarida. "Role of CFTR in the maintenance of airway epithelial cell differentiation". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  44. Pinto, MC; Lérias, JR; Botelho, Hugo M; Awatade, NT; Pepperkok, P; Kunzelmann, K; Amaral, Margarida. "Anoctamins 1 and 6 – Identification of novel traffic regulators and their significance to Cystic Fibrosis". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  45. Lérias, JR; Pinto, MC; Botelho, Hugo M; Schreiber, R; Amaral, Margarida; Kunzelmann, K. "Anoctamin 1 traffic and function is enhanced by tethering proteins family of extended synaptotagmins". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  46. Igreja, S; Botelho, Hugo M; Felício, V; Pagani, G; Pagani, M; Amaral, Margarida. "Validation of a CFTR alternative splicing reporter system for high-throughput microscopy and identification of novel CFTR regulators of alternative splicing". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  47. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Silva, Iris AL; Marques, Luis; Ramos, M; Amaral, Margarida. "CFTR modulators enhance function R334W-CFTR both in intestinal organoids and conditionally reprogrammed human nasal epithelial cells". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  48. Felício, V; Botelho, Hugo M; Igreja, S; Amaral, Margarida. "A high-throughput screening assay to identify factors correcting CFTR mutations bearing premature termination codons (PTCs)". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  49. Baptista, C; Vieira, H; Tenreiro, R; Awatade, NT; Amaral, Margarida; Botelho, Hugo M. "High throughput microscopy screens identify F508del-CFTR correctors and ANO1 traffic modulators in a unique Portuguese natural compound library". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  50. Botelho, Hugo M; Lim, SH; Tischer, C; Pepperkok, R; Stagljar, I; Amaral, Margarida. "A high content siRNA screen at near genomic scale reveals novel F508del-CFTR therapeutic targets". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017.
  51. Botelho, Hugo M; Awatade, NT; Tischer, C; Amaral, MD. "Modular high content image analysis workflows leveraging biology and biomedicine". Trabalho apresentado em NEUBIAS2020 BioImage Analysis Symposium, 2017.
  52. Botelho, Hugo M; Quaresma, MC; Amaral, Margarida. "CFTR in the maintenance of epithelial differentiation in the airways". Trabalho apresentado em Chem&BioChem Postgraduate Students Meeting, 2017.
  53. Igreja, S; Botelho, Hugo M; Amaral, Margarida. "Identification of novel regulators of alternative splicing by HT microscopy: implications for Cystic Fibrosis and other human diseases". Trabalho apresentado em EMBO Practical Course: High-Throughput Microscopy for Systems Biology, 2016.
  54. Pankonien, I; Santos-Sousa, L; Botelho, Hugo M; Amaral, Margarida. "Establishment of cell-based assays of airway epithelial differentiation and wound healing for automated microscopy". Trabalho apresentado em 13th ECFS Basic Science Conference, 2016.
  55. Lérias, JR; Afonso, Sara; Quaresma, MC; Pinto, M; Awatade, NT; Botelho, Hugo M; Schreiber, R; et al. "Anoctamin 1 - A member of a novel family of ion channels with extended functions and significance in disease". Trabalho apresentado em 13th ECFS Basic Science Conference, 2016.
  56. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Ramos, M; Amaral, Margarida. "To assess the efficacy of CFTR modulators on rare Cystic Fibrosis mutations using forskolin-induced swelling assay in human intestinal organoids". Trabalho apresentado em 13th ECFS Basic Science Conference, 2016.
  57. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Ramos, M; Amaral, Margarida. "Assessment of CFTR activity ex vivo as a basis for personalised medicine". Trabalho apresentado em 5th Workshop Structure and Function of Ion Channels and Transporters, 2015.
  58. Pankonien, I; Afonso, Sara; Quaresma, MC; Kmit, A; Dahimène, S; Botelho, Hugo M. "The epithelial transcription factor KLF4 affects the traffic of CFTR". Trabalho apresentado em 12th ECFS Basic Science Conference, 2015.
  59. Igreja, S; Botelho, Hugo M; Marques, Luis; Amaral, Margarida. "In vitro correction of the CFTR 2657+5G>A splicing mutation by an antisense oligonucleotide". Trabalho apresentado em 12th ECFS Basic Science Conference, 2015.
  60. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Awatade, NT; Proença, MC; Neumann, B; Tischer, C; et al. "Rescuing F508del-CFTR in airway epithelial cells using a novel traffic-based high content microscopy screening platform". Trabalho apresentado em 12th ECFS Basic Science Conference, 2015.
  61. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "A high content screening approach for the identification of CFTR traffic regulators in airway epithelial cells". Trabalho apresentado em ESF/EMBO Cell Polarity and Membrane Traffickin, 2014.
  62. Carvalho, SB; Botelho, Hugo M; Leal, SS; Cardoso, Isabel; Fritz, G; Gomes, Cláudio M. "Intrinsically disordered and aggregation prone regions underlie ß-aggregation in S100 proteins". Trabalho apresentado em 2nd Iberian Congress on Prions, 2013.
  63. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "High throughput screen reveals genes regulating wt-CFTR and rescuing F508del-CFTR traffic". Trabalho apresentado em 27th Annual North American Cystic Fibrosis Conference, 2013.
  64. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "Identification of CFTR traffic regulators as a source of novel Cystic Fibrosis therapeutic targets". Trabalho apresentado em The 5th EMBO Meeting, 2013.
  65. Sofia B Carvalho; Sónia S. Leal; Botelho, Hugo M; Isabel Cardoso; Günter Fritz; Cláudio M. Gomes. "Cross talks between amyloid-forming proteins in neurodegeneration". Trabalho apresentado em 9th EBSA European Biophysics Congress, 2013.
  66. Botelho, Hugo; Dahimène S; Uliyakina I; Neumann B; Tischer C; Pepperkok R; Amaral MD. "High throughput screening as a source for novel cystic fibrosis therapeutic targets". Trabalho apresentado em 36th European Cystic Fibrosis Conference, 2013.
  67. Dahimène S; Romão A; Botelho, Hugo; Palma M; Correia AR; Farinha CM; King-Underwood J; et al. "WS21.9 Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays". Trabalho apresentado em 36th European Cystic Fibrosis Conference, 2013.
  68. Bandeiras, T; Freitas, MC; Petrasch, D; Botelho, Hugo M; Gomes, Cláudio M; Kletzin, A; Frazão, Carlos. "Purification, crystallization and preliminary crystallographic analysis of Acidianus ambivalens Rieske ferredoxin 2 (RFd2), a new type of Rieske ferredoxins with an additional disulfide". Trabalho apresentado em 1° Encontro Nacional de Utilizadores de Radiação de Sincrotrão, 2012.
  69. Botelho, Hugo M; Christine Betz; Katrin Kierdorf; Marco Prinz; Cláudio M. Gomes; Günter Fritz. "A new emerging role of S100B in amyloid plaque formation". Trabalho apresentado em 56th Annual Meeting, German Society for Neuropathology and Neuroanatomy, 2011.
    10.5414/npp30245
  70. Bandeiras, T; Freitas, MC; Botelho, Hugo M; Gomes, Cláudio M; Carrondo, MA; Kletzin, A; Frazão, Carlos. "Crystallographic structure of Acidianus ambivalens Rieske ferredoxin 2 – The disulfide bridge structural role". Trabalho apresentado em EMBO Practical Course: High-throughput methods for Protein Production and Crystallization, 2011.
  71. Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA; Gomes, Cláudio M. "S100 proteins: modulators of Aß amyloidogenesis". Trabalho apresentado em Interbio Symposium 'Frontiers in Protein Research', 2011.
  72. Gomes, Cláudio M; Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA. "Metal ions and amyloid-beta peptide fibrils as modulators of S100 amyloidogenesis". Trabalho apresentado em FASEB Summer Research Conference - Protein Folding in the Cell, 2010.
  73. Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA; Gomes, Cláudio M. "Metal ions and Aß fibrils modulate S100 amyloidogenesis". Trabalho apresentado em IV Spanish-Portuguese Biophysics Congress, 2010.
  74. Almeida, M; Magalhães, LC; Sabino, FMR; Botelho, Hugo M; Gomes, Cláudio M. "Focused NMR structural proteomics on hyperstable proteome subsets". Trabalho apresentado em EUROMAR 2010, 2010.
  75. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Metal ions as modulators of folding and stability of the S100A2 tumor suppressor". Trabalho apresentado em Gordon Research Seminar & Gordon Research Conference “Protein Folding Dynamics”, 2010.
  76. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Calcium and zinc modulate the folding and stability of the S100A2 tumor suppressor". Trabalho apresentado em VIII European Symposium of The Protein Society, 2009.
  77. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Ca2+ and Zn2+ modulate the conformation and stability of the S100A2 tumor suppressor". Trabalho apresentado em The 9th European Biological Inorganic Chemistry Conference, EUROBIC9, 2008.
  78. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Role of Ca2+ and Zn2+ as modulators of the S100A2 tumor suppressor". Trabalho apresentado em FASEB Summer Research Conference – Protein Folding in the Cell, 2008.
  79. Nascimento, C; Botelho, Hugo M; Tavares de Almeida, I; Gomes, Cláudio M; Leandro P. "Folding rescue of mild PKU mutations by chemical chaperones". Trabalho apresentado em Society for the Study of Inborn Errors of Metabolism Symposium 2007, SSIEM Symposium 2007, 2007.
  80. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Conformational control of S100A2 by metal ions". Trabalho apresentado em 13th International conference on Biological Inorganic Chemistry, ICBIC XIII, 2007.
  81. Prosinecki, V; Botelho, Hugo M; Gomes, Cláudio M. "A proteomic approach to investigate protein stability". Trabalho apresentado em VII European Symposium of the Protein Society – From Proteins to Proteome, 2007.
  82. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Studies on the conformational effects of calcium and zinc binding to S100A2". Trabalho apresentado em VII European Symposium of the Protein Society – From Proteins to Proteome, 2007.
  83. Prosinecki, V; Botelho, Hugo M; Francese, S; Mastrobuoni, G; Moneti, G; Urich, T; Kletzin, A; Gomes, Cláudio M. "Mining for proteins with enhanced intrinsic thermal stability: a proteomic research on hyperthermophile Sulfurisphaera sp.". Trabalho apresentado em International Conference on Proteomics, PROTEOMLUX, 2006.
  84. Francese, S; Prosinecki, V; Botelho, Hugo M; Mastrobuoni, G; Pieraccini, G; Kletzin, A; Gomes, Cláudio M. "Proteomic characterization of hyperstable proteins within the soluble proteome of Sulfurisphaera sp.". Trabalho apresentado em 17th International Mass spectrometry Conference, 2006.
  85. Prosinecki, V; Botelho, Hugo M; Francese, S; Kletzin, A; Gomes, Cláudio M. "Ferredoxin and oxidative stress defence proteins are among the most stable proteins in the thermophile Sulfurisphaera sp.: a proteomic study". Trabalho apresentado em EUROBIC Young Researchers Forum and The 8th European Biological Chemistry Conference, EUROBIC8, 2006.
Resumo em conferência
  1. Dionisio Lopes, Margarida; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Screening de novos e antigos fármacos: um modulador para a prevenção de metástases ccerebrais do cancro da mama.". Trabalho apresentado em 2º Encontro Nacional de Jovens Investigadores em Oncologia, Porto, 2020.

Propriedade Intelectual

Patente
  1. Margarida D. Amaral; Botelho, Hugo M; Lopes-Pacheco, Miquéias. 2023. "Method of identifying agents for the treatment of cystic fibrosis caused by the mutation F508del".
    Concedida/Emitida
Pedido provisório de patente
  1. 2022. "Method of identifying agents for the treatment of cystic fibrosis caused by the mutation F508del".
    Protegido
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2023/10/25 High content screening methods and algorithms for basic and applied research on Cystic Fibrosis SPAOM2023 - Spanish & Portuguese Advanced Optical Microscopy Meeting
PPBI & REMOA (Faro, Portugal)
2023/06/10 Global functional genomics reveals GRK5 as a therapeutic target for cystic fibrosis 46th ECFS Conference
European Cystic Fibrosis Society (Vienna, Áustria)
2023/05/12 High content screening from a core facility perspective: from experiments to discoveries 5th NEUBIAS Symposium
Network of European BioImage Analysts (Porto, Portugal)
2022/04/01 New kinase therapeutic targets for Cystic Fibrosis from a global functional genomics screen 17th ECFS Basic Science Conference
European Cystic Fibrosis Conference (Albufeira, Portugal)
2022 37 lectures and workshops on Cystic Fibrosis, Microscopy and Bioimage Analysis at national and international events.
2021/11/03 Functional genomics reveals global genes rescuing F508del-CFTR traffic and novel kinase therapeutic targets complementing current correctors 35th North American Cystic Fibrosis Conference
Cystic Fibrosis Foundation (Estados Unidos)
2021/02/04 High-throughput microscopy and screening Optical microscopy at COLife and Friends: learning the basics
COLife (Lisboa, Portugal)
2020/11/26 VALHealth – Valorisation of ALgae for Health: Bioactive compounds from marine bioresources by membrane technology BioISI Research Day 2020
BioISI (Lisboa, Portugal)
2020/11/24 High content image analysis with CellProfiler SPAOM2020: Spanish Portuguese Meeting for Advanced Optical Microscopy
REMOA / PPBI (Valencia, Espanha)
2020/09/24 Screening de novos e antigos fármacos: um modulador para a prevenção de metástases cerebrais do cancro da mama 2º Encontro Nacional de Jovens Investigadores em Oncologia
Liga Portuguesa Contra o Cancro (Portugal)
2020/06/30 PPBI - Plataforma Portuguesa de BioImagem 6º Fórum de Gestão de Dados de Investigação
FCT-FCCN – Fundação para a Ciência e Tecnologia (Portugal)
2019/11/13 Breast cancer cells extravasation into the brain: a dynamic trafficking across blood-brain barrier endothelium 5th Annual Meeting of the Mind-Brain College of the University of Lisbon
University of Lisbon (Lisbon, Portugal)
2019/11/06 Analysis of time lapse microscopy data with CellProfiler et al. 3rd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2019
Red Española de Microscopía Óptica Avanzada & Portuguese Platform of BioImaging (Coimbra, Portugal)
2019/10/01 A systems view of Cystic Fibrosis: modeling the folding and maturation pathways of the CFTR protein 2nd CORBEL Service Operator and User Meeting
CORBEL (Heidelberg, Alemanha)
2018/12/06 shinyHTM: an open-source web application for the analysis of high throughput microscopy data EMBL Conference: From Images to Knowledge with ImageJ & Friends (I2K)
European Molecular Biology Laboratory (Heidelberg, Alemanha)
2018/10/26 Innovating the search for novel Cystic Fibrosis therapies using high content microscopy screening SPAOM2018: Spanish Portuguese Meeting for Advanced Optical Microscopy
Red Española de Microscopía Óptica Avanzada & Portuguese Platform of BioImaging (Granada, Espanha)
2018/01/22 A systems view of Cystic Fibrosis: modeling the folding and maturation pathways of the CFTR protein CORBEL Work Package 4 Service Operator meeting
CORBEL (Berlin, Alemanha)
2017/06/09 CFTR traffic – Functional genomics screens to identify novel regulators 40th European Cystic Fibrosis Conference
European Cystic Fibrosis Society (Seville, Espanha)
2017/04/01 A high-content siRNA screen at near genomic scale reveals novel F508del-CFTR therapeutic targets 14th ECFS Basic Science Conference
European Cystic Fibrosis Society (Albufeira, Portugal)
2017/02/15 Modular high content image analysis workflows leveraging biology and biomedicine NEUBIAS2020 BioImage Analysis Symposium
Network of European BioImage Analysts - NEUBIAS (Lisbon, Portugal)
2016/10/13 Innovating the search for novel Cystic Fibrosis therapies using high content microscopy screening BioISI Post Grad Seminar
Biosystems & Integrative Sciences Institute (Lisbon, Portugal)
2015/11/13 Assessment of CFTR activity ex vivo as a basis for personalised medicine 5th Structure and Function of Ionic Channels and Transporters Workshop
Faculty of Pharmacy of the University of Lisbon (Lisbon, Portugal)
2015/06/02 A high throughput fluorescence microscopy pipeline for drug discovery in Cystic Fibrosis 1st Course on High Content Screening and Image Analysis for Biosciences
i3S - Instituto de Investigação e Inovação da Universidade do Porto (Porto, Portugal)
2015/05/10 S100 proteins as novel metal-ion regulated molecular chaperones in neurodegeneration EMBO Conference: Molecular chaperones: From molecules to cells and misfolding diseases
European Molecular Biology Organization (Heraklion, Grécia)
2014/08/15 A high content screening system for the development of CFTR targeting therapies in the genetic disease Cystic Fibrosis International ITU Molecular Biology and Genetics Students Congress ‘14
Istanbul Technical University (Istanbul, Turquia)
2014/03/26 A high-content siRNA screens identify novel CFTR traffic regulators in human airway epithelial cells 11th ECFS Basic Science Conference
European Cystic Fibrosis Society (St. Julians, Malta)
2013/10/17 High throughput screen reveals genes regulating wt-CFTR and rescuing F508del-CFTR traffic 27th Annual North American Cystic Fibrosis Conference
Cystic Fibrosis Foundation (Salt Lake City, Estados Unidos)
2013/06/14 High throughput screening as a source for novel Cystic Fibrosis therapeutic targets 36th European Cystic Fibrosis Society Conference
European Cystic Fibrosis Society (Lisbon, Portugal)
2013/05/17 Cross talk between S100 proteins and amyloid-forming proteins in neurodegeneration EMBO Conference: The biology of molecular chaperones: From molecules, organelles and cells to misfolding diseases
European Molecular Biology Organization (Santa Margherita di Pula, Itália)
2013/05/14 Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays 36th European Cystic Fibrosis Society Conference
European Cystic Fibrosis Society (Lisbon, Portugal)
2013/03/07 High-throughput identification of CFTR traffic regulators BioFIG PhD Student/Post-Doc Seminar
Center for Biodiversity, Functional & Integrative Genomics (Lisbon, Portugal)
2011/01/19 Aß fibrils and metal ions modulate S100 amyloidogenesis: novel players in neurodegeneration? Lisbon Area Neuroscience Meeting
Instituto de Medicina Molecular (Lisbon, Portugal)
2010/07/08 Metal ions and Aß fibrils modulate S100 amyloidogenesis IV Spanish Portuguese Biophysical Congress
Sociedad de Biofísica de España, Portuguese Biophysical Society (Zaragoza, Espanha)
2009/11/04 Metal ions as modulators of folding and amyloidogenic pathways in the S100 protein family ITQB PhD Seminar
Instituto de Tecnologia Química e Biológica (Oeiras, Portugal)
2007/02/02 Modulation of the stability of S100A2 variants by metal ions Workshop Proteins in Health and Disease: from structure to function
Faculty of Pharmacy, University of Lisbon (Lisbon, Portugal)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2023/09/04 - 2024/09/01 Characterizing the regulation of the CFTR protein by the kinase GRK5 in cystic fibrosis models
Orientador
Bioquímica (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
2023/04/01 - 2023/10/01 Regulation of the intracellular traffic of p.Phe508del-CFTR by the kinase GRK5 in Cystic Fibrosis models
Orientador de Daniela Neugebauer
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2022/09 - 2023/07 Cristais de spin lábil termo e foto-sensíveis para músculos artificiais
Coorientador de André Fernandes
Química (Licenciatura/Bacharelato)
Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09/20 - 2022/07/15 Trainee bioimage analyst at BioISI’s High-Throughput Screening Facility
Orientador de Pau Rubio Costa
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2020/09/29 - 2022/01/31 Application of new bioactive compounds extracted from algae: health benefits and effects on Cystic Fibrosis
Coorientador de Ana Patrícia Rodrigues Duarte
Engenharia Química e Biológica (Mestrado)
Instituto Politécnico de Lisboa Instituto Superior de Engenharia de Lisboa, Portugal
2021/07/17 - 2021/12/31 Laboratory rotation, part of PhD course. Time-lapse confocal imaging of mixed 3D cell cultures. Optimization of a cellular extravasation assay
Orientador de Ana Rita da Costa Garcia
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/03/05 - 2021/03/31 Bioimage analyst at BioISI High-Throughput Screening Facility
Orientador de Aires Miguel Gomes Delgado de Almeida Duarte
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2020/12/03 - 2020/12/03 Laboratory rotation, part of PhD course. Confocal imaging of mixed 3D cell cultures; tools for 3D data visualization and cell tracking
Orientador de Joana Godinho Pereira
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/09/06 - 2020/09/17 Effect of Novel CFTR Interactors on Traffic of F508del Mutant: Implications for Cystic Fibrosis
Coorientador de Joana Cartaxo Serralha
Biomedical Science (Licenciatura/Bacharelato)
King's College London Faculty of Life Sciences and Medicine, Reino Unido

Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/02/01 - 2018/12/31 Deconvolution of dual CFTR/ANO1 Modulators from Portuguese natural products - A new class of drugs for CF therapy
Orientador de Joana Godinho-Pereira
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2016/05/01 - 2017/12/31 The identification of new natural compounds of high therapeutic potential for Cystic Fibrosis by high-throughput microscopy screens
Orientador de Catarina Duarte Galhardo Baptista
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2014 - 2015 Effect of TGFß signaling on CFTR protein: consequences for epithelial differentiation in CF
Coorientador de Margarida Cuco Quaresma
Bioquímica (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
2010 - 2011 Molecular and mechanistic aspects of the effect of metal ions in the conformation and amyloidogenesis of the S100B protein
Coorientador de Sofia Carvalho
Bioquímica (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2024/04/15 - 2024/04/19 Advanced Course on the Principles of Light Microscopy (2024/04/15 - 2024/04/19)
Oficina (workshop) (Membro da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2023/12/11 - 2023/12/13 Introduction to Image Analysis (2023/12/11 - 2023/12/13)
Oficina (workshop) (Membro da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2023/03 - 2023/10/27 SPAOM2023 – Spanish-Portuguese Advanced Optical Microscopy 2023 (2023/10/25 - 2023/10/27)
Congresso (Membro da Comissão Científica)
2023/08/28 - 2023/08/30 Training on confocal microscopy and core facility management (2023/08/28 - 2023/08/30)
Oficina (workshop) (Presidente da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2023/03/20 - 2023/03/22 Macro Scripting in ImageJ - COLife & FCUL (2023/03/20 - 2023/03/22)
2022/12/13 - 2022/12/16 Basics in Light Microscopy (2022/12/13 - 2022/12/16)
Oficina (workshop) (Presidente da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2022/09/21 - 2022/09/23 Introduction to Image Analysis - COLife & FCUL Course (2022/09/21 - 2022/09/23)
Oficina (workshop) (Membro da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2022/07/15 - 2022/07/15 Chem&BioChem – Postgraduate Students Meeting, FCUL (2022/07/15 - 2022/07/15)
Encontro (Membro da Comissão Científica)
Universidade de Lisboa Faculdade de Ciências, Portugal
2022/06/22 - 2022/06/22 Hands-on CellProfiler training for Lisbon Area Bioimaging Group members (2022/06/22 - 2022/06/22)
Oficina (workshop) (Presidente da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2022/01/18 - 2022/01/20 Macro Scripting in ImageJ - COLife & FCUL (2022/01/18 - 2022/01/20)
Oficina (workshop) (Coorganizador)
2021/11/23 - 2021/11/25 SPAOM2021 – Spanish-Portuguese Advanced Optical Microscopy 2021 (2021/11/23 - 2021/11/25)
Congresso (Membro da Comissão Científica)
Portuguese Platform of BioImaging, Portugal
2021/10/25 - 2021/10/28 Basics in Light Microscopy (2021/10/25 - 2021/10/28)
Oficina (workshop) (Presidente da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09/21 - 2021/09/23 Introduction to Image Analysis COLife & FCUL (2021/09/21 - 2021/09/23)
Oficina (workshop) (Coorganizador)
2021/02/15 - 2021/02/17 Eutopia 3 - Third Meeting of the European Topology Interdisciplinary Initiative (2021/02/15 - 2021/02/17)
Encontro (Membro da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2019/06/11 - 2019/06/12 Workshop on Integrative Approaches to Protein Folding & Aggregation (2019/06/11 - 2019/06/12)
Oficina (workshop) (Membro da Comissão Organizadora)
Universidade de Lisboa Faculdade de Ciências, Portugal
2018/07/09 - 2018/07/13 Hands-on Workshop on High-Throughput Microscopy (2018/07/09 - 2018/07/13)
Oficina (workshop) (Coorganizador)
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/07/17 - 2017/07/21 Hands-on Workshop on High-Throughput Microscopy (2017/07/17 - 2017/07/21)
Oficina (workshop) (Coorganizador)
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2016/07/11 - 2016/07/15 Hands-on Workshop on High-Throughput Microscopy (2016/07/11 - 2016/07/15)
Oficina (workshop) (Coorganizador)
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2015/03/18 - 2015/03/20 Hands-on Workshop on High-Throughput Microscopy (2015/03/18 - 2015/03/20)
Oficina (workshop) (Coorganizador)
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2022/03/30 - 2022/04/02 17th ECFS Basic Science Conference
Congresso
17th European Cystic Fibrosis Society Basic Science Conference
2021/11/30 - 2021/11/30 5.ª Conferência Anual da redeSAÚDE
Conferência
5.ª Conferência Anual da redeSAÚDE
Universidade de Lisboa, Portugal
2021/11/23 - 2021/11/25 SPAOM2021
Congresso
Spanish & Portuguese Advanced Optical Microscopy Meeting
2021/11/02 - 2021/11/05 35th NACFC
Congresso
35th North American Cystic Fibrosis Conference
2021/02/02 - 2021/02/04 Optical microscopy at COLife & Friends: learning the basics
Oficina (workshop)
Optical microscopy at COLife and Friends: learning the basics
2020/11/24 - 2020/11/27 SPAOM2020
Congresso
Spanish & Portuguese Advanced Optical Microscopy Meeting
2020/09/24 - 2020/09/25 43rd ECFS Conference
Congresso
43rd European Cystic Fibrosis Conference
2020/06/30 - 2020/07/01 6º Fórum GDI
Conferência
6º Fórum de Gestão de Dados de Investigação
Portuguese Platform of BioImaging, Portugal
2019/11/06 - 2019/11/08 SPAOM2019
Encontro
3rd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2019
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/10/01 - 2019/10/02 2nd CORBEL Service Operator and User Meeting
Encontro
2nd CORBEL Service Operator and User Meeting
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/06/19 - 2019/06/19 Preclinical Imaging Lifesciences
Oficina (workshop)
Preclinical Imaging Lifesciences
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/12/05 - 2018/12/08 From images to knowledge with ImageJ & friends (I2K)
Conferência
EMBL Conference: From images to knowledge with ImageJ & friends (I2K) and I2K Developers Satellite Meeting
European Molecular Biology Laboratory, Alemanha
2018/10/23 - 2018/10/26 SPAOM2018
Encontro
2nd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2018
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/02/22 - 2018/02/23 CORBEL WP4 Service Operator Meeting
Encontro
CORBEL WP4 Service Operator Meeting
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/09/17 - 2017/09/21 EMBO Workshop
Oficina (workshop)
EMBO Workshop – Proteostasis
European Molecular Biology Organization, Alemanha
2017/06/07 - 2017/06/10 40th European Cystic Fibrosis Conference
Conferência
40th European Cystic Fibrosis Conference
European Cystic Fibrosis Society, Dinamarca
2017/06/06 - 2017/06/09 3rd Course on High Content Screening and Image Analysis for BioSciences
Outro
3rd Course on High Content Screening and Image Analysis for BioSciences
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017/05/08 - 2017/05/08 3rd Annual Conference Rede Mar
Conferência
3rd Annual Conference Rede Mar
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/03/29 - 2017/04/01 14th ECFS Basic Science Conference
Conferência
14th ECFS Basic Science Conference
European Cystic Fibrosis Society, Dinamarca
2017/02/15 - 2017/02/17 NEUBIAS2020 BioImage Analysis Symposium
Simpósio
NEUBIAS2020 BioImage Analysis Symposium
European Cooperation in Science and Technology, Bélgica
2016/07/04 - 2016/07/06 Ciência 2016
Encontro
Ciência 2016 - Science and Technology in Portugal Meeting
Fundação para a Ciência e a Tecnologia, Portugal
2016/06/14 - 2016/06/17 2nd Course on High Content Screening and Image Analysis for Biosciences
Outro
2nd Course on High Content Screening and Image Analysis for Biosciences
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/06/01 - 2015/06/04 1st Course on High Content Screening and Image Analysis for Biosciences
Outro
1st Course on High Content Screening and Image Analysis for Biosciences
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/03/25 - 2015/03/28 12th ECFS Basic Science Conference
Conferência
12th ECFS Basic Science Conference
European Cystic Fibrosis Society, Dinamarca
2014/08/15 - 2014/08/18 ITU Molecular Biology and Genetics Students Congress
Congresso
International ITU Molecular Biology and Genetics Students Congress ’14
Istanbul Teknik Üniversitesi, Turquia

European Molecular Biology Organization, Alemanha
2014/05/10 - 2014/05/15 ESF/EMBO Cell Polarity and Membrane Trafficking
Conferência
ESF/EMBO Cell Polarity and Membrane Trafficking
European Molecular Biology Organization, Alemanha

European Science Foundation, França
2013/10/17 - 2013/10/19 27th Annual NACFC
Conferência
27th Annual North American Cystic Fibrosis Conference
Cystic Fibrosis Foundation, Estados Unidos

European Molecular Biology Organization, Alemanha
2013/09/21 - 2013/09/24 The 5th EMBO Meeting
Encontro
The 5th EMBO Meeting
European Molecular Biology Organization, Alemanha
2013/07/18 - 2013/07/18 Molecular Biology in Portugal and EMBL
Encontro
Molecular Biology in Portugal and EMBL
Universidade de Lisboa Faculdade de Ciências, Portugal
2013/06/12 - 2013/06/15 36th ECFS Conference
Conferência
36th European Cystic Fibrosis Society Conference
European Cystic Fibrosis Society, Dinamarca

Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Alemanha
2011/10/26 - 2011/10/28 2nd ITQB PhD Students’ Meeting
Encontro
2nd ITQB PhD Students’ Meeting
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Alemanha
2011/09/21 - 2011/09/24 56th Annual Meeting DGNN
Encontro
56th Annual Meeting, German Society for Neuropathology and Neuroanatomy
Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Alemanha
2011/05/05 - 2011/05/07 Interbio Symposium
Simpósio
Interbio Symposium 'Frontiers in Protein Research'
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/10/21 - 2010/10/22 1st ITQB PhD Students' Meeting
Encontro
1st ITQB PhD Students' Meeting
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/07/07 - 2010/07/10 IV Spanish-Portuguese Biophysical Congress
Congresso
IV Spanish-Portuguese Biophysical Congress
Sociedade Portuguesa de Biofísica, Portugal

Sociedad de Biofísica de España, Espanha
2010/01/10 - 2010/01/15 Gordon Research Conference
Conferência
Gordon Research Conference “Protein Folding Dynamics”
2010/01/09 - 2010/01/10 Gordon Research Seminar
Seminário
Gordon Research Seminar “Protein Folding Dynamics”
2009/10/30 - 2009/11/01 8th Short Course of the Portuguese Biophysical Society
Outro
8th Short Course of the Portuguese Biophysical Society “Systems Biology”
Sociedade Portuguesa de Biofísica, Portugal
2009/06/14 - 2009/06/18 VIII European Symposium of The Protein Society
Simpósio
VIII European Symposium of The Protein Society
Protein Society, Estados Unidos
2009/03/19 - 2009/03/19 Protein Folding Diseases: From Metabolic Disorders to Neurodegeneration
Oficina (workshop)
International Workshop “Protein Folding Diseases: From Metabolic Disorders to Neurodegeneration”
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2008/10/02 - 2008/10/03 Workshop Physics of Biological Systems
Oficina (workshop)
Workshop Physics of Biological Systems: From Genes to Societies
Universidade de Lisboa Centro de Física Teórica e Computacional, Portugal
2008/09/02 - 2008/09/06 EUROBIC9
Conferência
The 9th European Biological Inorganic Chemistry Conference, EUROBIC9
Society of Biological Inorganic Chemistry, Estados Unidos
2008/07/27 - 2008/08/01 FASEB Summer Research Conference
Conferência
FASEB Summer Research Conference – Protein Folding in the Cell
Federation of American Societies for Experimental Biology, Estados Unidos
2008/03/07 - 2008/03/07 1st Symposium iMed.UL
Simpósio
1st Symposium iMed.UL
Universidade de Lisboa Faculdade de Farmácia, Portugal
2007/07/15 - 2007/07/20 ICBIC XIII
Conferência
13th International conference on Biological Inorganic Chemistry, ICBIC XIII
Society of Biological Inorganic Chemistry, Estados Unidos
2007/05/12 - 2007/05/16 VII European Symposium of the Protein Society
Simpósio
VII European Symposium of the Protein Society – From Proteins to Proteome
Protein Society, Estados Unidos
2007/02/02 - 2007/02/03 Workshop Proteins in Health and Disease
Oficina (workshop)
Workshop Proteins in Health and Disease: from Structure to Function
Universidade de Lisboa Faculdade de Farmácia, Portugal
2006/11/08 - 2006/11/08 A one day workshop on protein science
Oficina (workshop)
A one day workshop on protein science
Universidade de Lisboa Centro de Física Teórica e Computacional, Portugal
2006/07/01 - 2006/07/06 EUROBIC8
Conferência
EUROBIC8 – 8th European Biological Inorganic Chemistry Conference and Young Researchers Forum
Society of Biological Inorganic Chemistry, Estados Unidos

Júri de grau académico

Tema
Tipo de participação
Nome do candidato (Tipo de grau)
Instituição / Organização
2022/03/21 Amyloids in Nature: structural analysis of sea urchin inspired adhesive protein
Arguente principal
André Gonzaga Ribeiro de Castro Fernandes (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
2022/03/21 Amyloids in Nature: structural analysis of sea urchin inspired adhesive protein
Arguente principal
André Gonzaga Ribeiro de Castro Fernandes (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal

Arbitragem científica em revista

Nome da revista (ISSN) Editora
2023/05/02 - Atual Healthcare (2227-9032) MDPI
2023/03 - Atual Cells (2073-4409) MDPI
2023 - Atual Journal of Microscopy (1365-2818) Wiley
2023 - Atual International Journal of Molecular Sciences (1422-0067) MDPI
2022/11 - Atual Medicina (1648-9144) MDPI
2021 - Atual Molecular Genetics & Genomic Medicine (2324-9269) Wiley Periodicals, LLC
2021 - Atual Journal of Visualized Experiments (1940-087X)
2018/11 - Atual SLAS DISCOVERY Advancing the Science of Drug Discovery (2472-5560) SAGE Publications
2016/11 - Atual Scientific Reports (2045-2322) Springer Science and Business Media LLC
2014/11 - Atual PLoS ONE (1932-6203) Public Library of Science
2013/08 - Atual Biochimie (0300-9084) Elsevier
2013/02 - Atual Metallomics (1756-591X) The Royal Society of Chemistry
2012/12 - Atual FEBS Letters (1873-3468) Wiley (John Wiley & Sons)
2012/12 - Atual Journal of Dermatological Science (0923-1811) Elsevier
2011 - Atual Physical Chemistry Chemical Physics (1463-9076) Royal Society of Chemistry
2011 - Atual Journal of Biotechnology (0168-1656) Elsevier

Comissão de avaliação

Descrição da atividade
Tipo de assessoria
Instituição / Organização Entidade financiadora
2021/07/29 - Atual Maria Joana Valente Ribeiro Main opponent: Seminário de Investigação I (1st year PhD discussion)
Avaliador
Universidade de Lisboa Faculdade de Ciências, Portugal
2021 - Atual Effective jury member for hiring an MSc student for CFF grant “Rep2CFTR: Repurposing FDA-Approved Drugs for Class II Rare CFTR Mutants”. PI: Miquéias Lopes-Pacheco
Avaliador
Universidade de Lisboa Faculdade de Ciências, Portugal
2020/02/21 - Atual Jury member for hiring a PhD researcher for FCT project “ProDysMito” Reference PTDC/BIA-BQM/29963/2017 PI: Bárbara J Henriques
Avaliador
Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal Fundação para a Ciência e a Tecnologia

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2024 - Atual Experimental Biochemistry IV Bioquímica (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal
2022/02 - 2024/07 Fundamentos de Química e Bioquímica [Fundamentals in Chemistry and Biochemistry] Engenharia Biomédica e Biofísica (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal
2023/02 - 2023/07 Introdução à Biologia Molecular [Introduction to Molecular Biology] Bioquímica (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal
2023/02 - 2023/07 Projeto [Project] Química (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal
2020/02 - 2020/06 Biochemistry Biologia (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal

Membro de comissão

Descrição da atividade
Tipo de participação
Instituição / Organização
2023/12 - Atual Chair of Image Analysis Working Group
Coordenador
Portuguese Platform of BioImaging, Portugal
2022 - Atual Member of PhD thesis advisory committee - Maria Joana Valente Ribeiro PhD in Biochemistry
Membro
Universidade de Lisboa Faculdade de Ciências, Portugal
2019/07 - Atual High-Throughput Screening Facility, Manager
Coordenador
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/07 - Atual FCUL Microscopy Facility, Co-Responsible
Coordenador
Universidade de Lisboa Faculdade de Ciências, Portugal

Tutoria

Tópico Nome do aluno
2011/01/01 - 2011/01/31 Conformation and Stability of Phenylalanine Hydroxylase João Leandro
2010/05/01 - 2010/12/31 Molecular basis of the stabilization of the Acidianus ambivalens Rieske Ferredoxin by using rationally designed mutants Francesco Barbero
2010/03 - 2010/03 Elucidation of the molecular basis of the stabilization of the Acidianus ambivalens Rieske Ferredoxin by using rationally designed mutants Dennis Petrasch
2009/10/01 - 2009/10/31 Assembly of wild type and mutant Acidianus ambivalens Sulfur Oxygenase Reductase Andreas Veith
2007/01/01 - 2007/03/31 Conformation and stability of Phenylalanine Hydroxylase Cátia Nascimento
Distinções

Prémio

2019 Travel grant. NEUBIAS Training School #13. BioImage Analysis for Facility Staff
2018 ITC Conference Grant - EMBL Conference "From Images to Knowledge with ImageJ & Friends"
European Cooperation in Science and Technology, Bélgica
2015 Meeting fellowship - 12th ECFS Basic Science Conference
European Cystic Fibrosis Society, Dinamarca
2013 Best Poster Award at the meeting “Molecular Biology in Portugal and EMBL"
2011 FEBS Short Term Grant
Federation of European Biochemical Societies, Reino Unido
2010 Subsidy for participating in International Congresses - IV Spanish-Portuguese Biophysical Congress)
Fundação Calouste Gulbenkian, Portugal
2010 Travel Grant - IV Spanish-Portuguese Biophysical Congress
2010 Travel Grant - IV Spanish-Portuguese Biophysical Congress
2007 Banco Espírito Santo Award – Best Classified Graduates 2005/2006 – Biochemistry Course, Faculty of Sciences, University of Lisbon
Banco Espírito Santo e Comercial de Lisboa, Portugal
2007 Student Travel Grant - 13th International Conference on Biological Inorganic Chemistry
Society of Biological Inorganic Chemistry, Estados Unidos
2004 Fundação da Faculdade de Ciências da Universidade de Lisboa Award – Best Classified 1st year Graduation Students in 2002/2003
Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal

Outra distinção

2014 Post-Doc Fellowship (SFRH/BD/31126/2006)
Fundação para a Ciência e a Tecnologia, Portugal
2012 Post-Doc Fellowship (Reference #7207534)
Cystic Fibrosis Foundation, Estados Unidos
2011 Post-Doc Fellowship (Research Project PTDC/QUI/70101/2006)
Fundação para a Ciência e a Tecnologia, Portugal
2006 PhD Fellowship (SFRH/BD/31126/2006)
Fundação para a Ciência e a Tecnologia, Portugal