Hugo Miguel Raposo Correia Botelho (E218-E579-24C1)

Curriculum Vitae

Hugo Miguel Raposo Correia Botelho

Hugo M. Botelho is a Junior Researcher at the Biosystems & Integrative Sciences Institute (BioISI) of the Faculty of Sciences University of Lisbon (FCUL) and Manager of BioISI's High-Throughput Screening (HTS) Facility. Scientist with a strong expertise in microscopy, cell biology, bioimage analysis, drug discovery and protein folding in vivo and in vitro. Obtained his PhD in Biochemistry (specialty: biophysics) in 2010 under the supervision of Cláudio M. Gomes (ITQB/UNL) for studies on protein folding and aggregation. In 2012 joined Margarida Amaral's lab at FCUL to research novel therapeutics for Cystic Fibrosis (CF). For 1.5 years was trained on high-throughput microscopy, RNA interference screening and membrane trafficking at EMBL Heidelberg. Since 2014 he has been employing siRNA-based screens to characterize novel therapeutic CF target genes. As principal investigator he has coordinated research projects focusing on the identification and characterization of novel molecules with therapeutic relevance for CF using natural compound sources and HTS methodologies. Since 2014 he has established a HTS Facility at BioISI and now serves as its manager, which includes supervision of 1 research technician. Each year the facility provides 5000 hours of equipment access time to about 40 users regarding sample preparation, imaging, data analysis and screening assay services. He is also actively engaged in developing and deploying automated image analysis algorithms for high content microscopy, software development and data science approaches to biological data. Has authored 29 original peer reviewed publications, 4 book chapters, supervised students and post-docs and been involved in several scientific research projects, both as team member and principal investigator. As manager of the HTS Facility, represents BioISI and FCUL at the Portuguese Platform of BioImaging (PPBI), PT-OPENSCREEN, Euro-BioImaging ERIC, EU-OPENSCREEN, the Lisbon Area BioImaging Group, among others. He is a regular trainer and speaker at microscopy and CF meetings and courses. h-index 14 (Scopus, May 2023)

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Identificação

Identificação pessoal

Nome completo: Hugo Miguel Raposo Correia Botelho
Género: Masculino

Nomes de citação

Botelho, Hugo M

Identificadores de autor

Ciência ID: E218-E579-24C1
ORCID iD: 0000-0002-4208-1086
Google Scholar ID: nXftyYwAAAAJ&hl
Researcher Id: B-3092-2008
Scopus Author Id: 15030102200

Endereços de correio eletrónico

hmbotelho@ciencias.ulisboa.pt (Profissional)

Telefones

Telefone

217500659 (Profissional)

Moradas

BioISI - Biosystems & Integrative Sciences Institute. Campo Grande, C8, 1749-016, Lisboa, Lisboa, Portugal (Profissional)

Websites

http://webpages.fc.ul.pt/~hmbotelho (Académico)
http://bioisi.pt/hts (Académico)
https://www.researchgate.net/profile/Hugo_Botelho (Rede social)
https://github.com/hmbotelho (Rede social)
https://www.linkedin.com/in/hugo-botelho-b605b927 (Rede social)

Domínios de atuação

Ciências Naturais - Ciências Biológicas - Biologia Celular
Ciências Exatas - Ciências da Computação e da Informação
Ciências Médicas e da Saúde - Biotecnologia Médica
Ciências Naturais - Ciências Biológicas - Biofísica

Idiomas

Idioma	Conversação	Leitura	Escrita	Compreensão	Peer-review
Inglês	Utilizador proficiente (C1)	Utilizador proficiente (C1)	Utilizador proficiente (C1)	Utilizador proficiente (C1)	Utilizador proficiente (C1)
Francês	Utilizador elementar (A1)	Utilizador elementar (A1)	Utilizador elementar (A1)	Utilizador elementar (A1)	Utilizador elementar (A1)
Espanhol; Castelhano	Utilizador elementar (A1)	Utilizador elementar (A1)	Utilizador elementar (A1)	Utilizador elementar (A1)	Utilizador elementar (A1)
Português (Idioma materno)

Formação

	Grau	Classificação
2010/11/22 Concluído	Biochemistry (Doutoramento) Especialização em Biophysics Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal "Metal Ions and Protein Folding - Conformational and Functional Interplay" (TESE/DISSERTAÇÃO)	Approved by unanimity
2006/07/14 Concluído	Bioquímica (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal "Pesquisa, identificação e caracterização de proteínas hiperestáveis no proteoma solúvel da Archaea hipertermofílica Sulfurisphaera sp." (TESE/DISSERTAÇÃO)	18

Percurso profissional

Ciência

	Categoria Profissional Instituição de acolhimento	Empregador
2019/07/01 - Atual	Investigador Contratado (Investigação)	Universidade de Lisboa Faculdade de Ciências, Portugal
	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2015/05/01 - 2019/06/30	Pós-doutorado (Investigação)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

2012/04/15 - 2019/06/30	Pós-doutorado (Investigação)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

2012/07/16 - 2013/12/31	Investigador visitante (Investigação)	European Molecular Biology Laboratory, Alemanha

2012 - 2013	Investigador (Investigação)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

2011/09/01 - 2012/04/14	Pós-doutorado (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2011/07/03 - 2011/10/03	Pós-doutorado (Investigação)	Universitätsklinikum Freiburg Neurologische Universitätsklinik, Alemanha

2010/11/23 - 2011/06/30	Pós-doutorado (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2011 - 2011	Investigador (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2010/01/25 - 2010/02/07	Investigador visitante (Investigação)	Umea Universitet, Suécia

2005/09/05 - 2006/07/14	Estagiário de Investigação (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Outros

	Categoria Profissional Instituição de acolhimento	Empregador
2006/10/01 - 2010/11/22	PhD Student	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Projetos

Bolsa

	Designação	Financiadores
2016/05/01 - 2018/01/01	The identification of new natural compounds of high therapeutic potential for Cystic Fibrosis by high-throughput microscopy screens BioISI Post-Doc Orientador Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluído

Projeto

	Designação	Financiadores
2024/01/01 - Atual	ORGESTRA - Organoid technologies for disease modeling, drug discovery and development for rare diseases 101120108 Horizon Europe, Bélgica	Em curso
2022/02/24 - Atual	ISIDORe - Integrated Services for Infectious Diseases Outbreak Research 101046133 Investigador Universidade de Lisboa Faculdade de Ciências, Portugal	European Union Em curso
2024/02 - 2024/09	Biocompatible Coatings with Natural-Based Nano-Agents for Biomedical Applications BioISI Projects 2024 Co-Investigador Responsável (Co-IR) Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Em curso
2023/01/01 - 2024/06/30	NewKinCF - Unraveling the mechanism of action of a novel kinase regulator of F508del-CFTR traffic and activity 2022.03453.PTDC Investigador responsável Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Em curso
2022/05 - 2023/04	Mitochondrial network in Multiple Acyl-CoA Dehydrogenase Deficiency: construction of a high-content bioimage analysis workflow BioISI Projects 2022 Investigador responsável Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2017/06/01 - 2021/10/31	PPBI - Portuguese Platform of BioImage PPBI-POCI-01-0145-FEDER-022122 Investigador Universidade de Lisboa Faculdade de Ciências, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2018/10/01 - 2021/09/30	Identificação de Novos Fármacos para Fibrose Quística PTDC/MED-QUI/28800/2017	Fundação para a Ciência e a Tecnologia Em curso
2020/02/04 - 2021/03/31	VALHealth - Valorisation of Algae for Health: Bioactive Compounds from Marine Bioresources by Membrane Technology BioISI Projects 2020 Investigador responsável Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluído
2018 - 2021	AbrogateBM: Detect, prevent, erradicate: a multi-step strategy to abrogate brain metastasization of breast cancer PTDC/MED-ONC/29402/2017 Consultant Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal	Associação para a Inovação e Desenvolvimento da FCT Concluído
2015/09/01 - 2020/05/31	CORBEL - Coordinated Research Infrastructures Building Enduring Life-science Services 654248 VIP User Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	European Union Concluído
2019/01/01 - 2019/12/31	Instituto de Biosistemas & Ciências Integrativas UID/Multi/04046/2019 Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal Universidade do Minho, Portugal Universidade do Porto Faculdade de Ciências, Portugal Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal FCiênciasID Associação para a Investigação e Desenvolvimento de Ciências, Portugal Universidade de Lisboa Faculdade de Ciências, Portugal Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2016 - 2019	Novel CFTR Traffic Factors Related with Epithelial Cell Differentiation: Potential Therapeutic Targets for Cystic Fibrosis PTDC/BIM-MEC/2131/2014 Investigador Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2018/02/01 - 2018/12/31	Deconvolution of dual CFTR/ANO1 Modulators from Portuguese natural products - A new class of drugs for CF therapy BioISI Projects 2018 Investigador responsável Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluído
2016 - 2018	MIMED - Mining the molecular metric space for drug design PTDC/EEI-ESS/4923/2014 Investigador Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2017/01/07 - 2017/12/30	A new class of drugs for CF therapy - Dual CFTR/ANO1 Modulators from Portuguese natural products BioISI Projects 2017 Investigador responsável Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluído
2014 - 2017	Innovative non-CFTR approaches to cystic fibrosis therapy Strategic Research Centre Grant Investigador Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Cystic Fibrosis Trust Concluído
2016/01/05 - 2016/12/16	Natural compounds as a source of novel drug leads for Cystic Fibrosis BioISI Projects 2016 Investigador responsável Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas Concluído
2011 - 2013	Identification of novel targets rescuing of F508del-CFTR traffic: Mechanism of action 7207534 Investigador Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal	Cystic Fibrosis Foundation Concluído
2011/01/01 - 2012/12/31	Strategic Project - LA 4 - 2011-2012 PEst-OE/EQB/LA0004/2011 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal Fundação Calouste Gulbenkian, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal Universidade NOVA de Lisboa NOVA Medical School, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2012/02/01 - 2012/04/15	Mechanisms of SOD1 toxic aggregation in neurodegenerative processes info:eu-repo/grantAgreement/FCT/3599-PPCDT/117789/PT Investigador Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2011 - 2012	Amyloidogenesis of the S100A8/A9 cytokine as an anti-proliferative mechanism in prostate cancer Terry Fox Investigador Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Liga Portuguesa Contra o Cancro Concluído
2008/01/02 - 2011/12/31	Towards the understanding of the energetic and structural interplay between metal sites and protein folding info:eu-repo/grantAgreement/FCT/3599-PPCDT/70101/PT Investigador Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2007/07/01 - 2011/06/30	Functional analysis and origins of the pathophysiology of mutations within genes of the fatty acid oxidation pathway: implications in the multiple acyl-CoA dehydrogenase deficiency disorder info:eu-repo/grantAgreement/FCT/3599-PPCDT/70033/PT Investigador Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluído

Produções

Publicações

Artigo em revista	Mariana Coelho; Ana Patrícia Duarte; Sofia Pinto; Hugo M. Botelho; Catarina Pinto Reis; Maria Luísa Serralheiro; Rita Pacheco. "Edible Seaweeds Extracts: Characterization and Functional Properties for Health Conditions". Antioxidants 12 3 (2023): 684. https://doi.org/10.3390/antiox12030684. Publicado • 10.3390/antiox12030684 Margarida C Quaresma; Hugo M Botelho; Ines Pankonien; Cláudia S Rodrigues; Madalena C Pinto; Pau R Costa; Aires Duarte; Margarida D Amaral. "Exploring YAP1-centered networks linking dysfunctional CFTR to epithelial–mesenchymal transition". Life Science Alliance (2022): https://doi.org/10.26508/lsa.202101326. 10.26508/lsa.202101326 Joana Godinho-Pereira; Margarida Dionísio Lopes; Ana Rita Garcia; Hugo M. Botelho; Rui Malhó; Inês Figueira; Maria Alexandra Brito. "A Drug Screening Reveals Minocycline Hydrochloride as a Therapeutic Option to Prevent Breast Cancer Cells Extravasation across the Blood–Brain Barrier". Biomedicines (2022): https://doi.org/10.3390/biomedicines10081988. 10.3390/biomedicines10081988 João F. Ferreira; Iris A. L. Silva; Hugo M. Botelho; Margarida D. Amaral; Carlos M. Farinha. "Absence of EPAC1 Signaling to Stabilize CFTR in Intestinal Organoids". Cells (2022): https://doi.org/10.3390/cells11152295. 10.3390/cells11152295 Célia Fernandes; Elisa Palma; Francisco Silva; Ana Belchior; Catarina I. G. Pinto; Joana F. Guerreiro; Hugo M. Botelho; et al. "Searching for a Paradigm Shift in Auger-Electron Cancer Therapy with Tumor-Specific Radiopeptides Targeting the Mitochondria and/or the Cell Nucleus". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms23137238. 10.3390/ijms23137238 Pinto, Madalena C.; Botelho, Hugo M; Silva, Iris AL; Violeta Railean; Beate Neumann; Rainer Pepperkok; Rainer Schreiber; Karl Kunzelmann; Amaral, Margarida. "Systems Approaches to Unravel Molecular Function: High-content siRNA Screen Identifies TMEM16A Traffic Regulators as Potential Drug Targets for Cystic Fibrosis". Journal of Molecular Biology 434 5 (2022): 167436-167436. http://dx.doi.org/10.1016/j.jmb.2021.167436. Publicado • 10.1016/j.jmb.2021.167436 Sang Hyun Lim; Jamie Snider; Liron Birimberg-Schwartz; Wan Ip; Joana C Serralha; Botelho, Hugo M; Miquéias Lopes-Pacheco; et al. "CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system". Molecular Systems Biology 18 2 (2022): https://doi.org/10.15252/msb.202110629. 10.15252/msb.202110629 Marne C Hagemeijer; Annelotte M Vonk; Nikhil T Awatade; Iris A L Silva; Christian Tischer; Volker Hilsenstein; Jeffrey M Beekman; et al. Autor correspondente: Hugo M Botelho. "An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay". Bioinformatics (2020): https://doi.org/10.1093/bioinformatics/btaa1073. 10.1093/bioinformatics/btaa1073 Silva IAL; Doušová T; Ramalho S; Centeio R; Clarke LA; Railean V; Botelho, Hugo M; et al. "Organoids as a personalized medicine tool for ultra-rare mutations in cystic fibrosis: The case of S955P and 1717-2A>G.". Biochimica et biophysica acta. Molecular basis of disease 1866 (2020): 165905. http://europepmc.org/abstract/med/32730979. Publicado • 10.1016/j.bbadis.2020.165905 Inna Uliyakina; Botelho, Hugo M; Ana C. da Paula; Sara Afonso; Miguel J. Lobo; Verónica Felício; Carlos M. Farinha; Margarida D. Amaral. "Full Rescue of F508del-CFTR Processing and Function by CFTR Modulators Can Be Achieved by Removal of Two Regulatory Regions". International Journal of Molecular Sciences 21 12 (2020): 4524-4524. https://doi.org/10.3390/ijms21124524. Publicado • 10.3390/ijms21124524 Amaral, Margarida D.; Hutt, Darren M; Tomati, Valeria; Botelho, Hugo M; Pedemonte, Nicoletta. "CFTR processing, trafficking and interactions". Journal of Cystic Fibrosis 19 (2020): S33-S36. http://dx.doi.org/10.1016/j.jcf.2019.10.017. Publicado • 10.1016/j.jcf.2019.10.017 João D. Santos; Sara Canato; Ana S. Carvalho; Botelho, Hugo M; Kerman Aloria; MD Amaral; Rune Matthiesen; Andre O. Falcao; Carlos Farinha. "Folding Status Is Determinant over Traffic-Competence in Defining CFTR Interactors in the Endoplasmic Reticulum". Cells 8 4 (2019): 353. https://www.mdpi.com/2073-4409/8/4/353. Publicado • 10.3390/cells8040353 Awatade, Nikhil T.; Ramalho, Sofia; Silva, Iris A.L.; Felício, Verónica; Botelho, Hugo M; de Poel, Eyleen; Vonk, Annelotte; et al. "R560S: A class II CFTR mutation that is not rescued by current modulators". Journal of Cystic Fibrosis 18 2 (2019): 182-189. http://dx.doi.org/10.1016/j.jcf.2018.07.001. Publicado • 10.1016/j.jcf.2018.07.001 Palma, Elisa; Botelho, Hugo M; Morais, Goreti Ribeiro; Rodrigues, Inês; Santos, Isabel Cordeiro; Campello, Maria Paula Cabral; Raposinho, Paula; et al. "Unravelling the antitumoral potential of novel bis(thiosemicarbazonato) Zn(II) complexes: structural and cellular studies". JBIC Journal of Biological Inorganic Chemistry 24 1 (2018): 71-89. http://dx.doi.org/10.1007/s00775-018-1629-6. Publicado • 10.1007/s00775-018-1629-6 Joana S. Cristóvão; Vanessa K. Morris; Isabel Cardoso; Sónia S. Leal; Javier Martínez; Botelho, Hugo M; Christoph Göbl; et al. "The neuronal S100B protein is a calcium-tuned suppressor of amyloid-ß aggregation". Science Advances 4 6 (2018): eaaq1702-eaaq1702. https://doi.org/10.1126/sciadv.aaq1702. Publicado • 10.1126/sciadv.aaq1702 Lérias, Joana R.; Pinto, Madalena C.; Botelho, Hugo M; Awatade, Nikhil T.; Quaresma, Margarida C.; Silva, Iris A.L.; Wanitchakool, Podchanart; et al. "A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1865 2 (2018): 421-431. http://dx.doi.org/10.1016/j.bbamcr.2017.11.009. Publicado • 10.1016/j.bbamcr.2017.11.009 Botelho, Hugo; Amaral, Margarida D.; Farinha, Carlos M.; Matos, Paulo; Botelho, Hugo M.. "Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).". Methods in molecular biology (Clifton, N.J.) (2016): 105-126. http://europepmc.org/abstract/med/27665554. Publicado • 10.1007/978-1-4939-3804-9_7 Igreja, Susana; Clarke, Luka A.; Botelho, Hugo M; Marques, Luís; Amaral, Margarida D.. "Correction of a Cystic Fibrosis Splicing Mutation by Antisense Oligonucleotides". Human Mutation 37 2 (2015): 209-215. http://dx.doi.org/10.1002/humu.22931. Publicado • 10.1002/humu.22931 Clarke, Luka A.; Botelho, Hugo M; Sousa, Lisete; Falcao, Andre O.; Amaral, Margarida D.. "Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators". Genomics 106 5 (2015): 268-277. http://dx.doi.org/10.1016/j.ygeno.2015.07.005. Publicado • 10.1016/j.ygeno.2015.07.005 Botelho, Hugo M; Uliyakina, Inna; Awatade, Nikhil T.; Proença, Maria C.; Tischer, Christian; Sirianant, Lalida; Kunzelmann, Karl; Pepperkok, Rainer; Amaral, Margarida D.. "Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery". Scientific Reports 5 1 (2015): http://dx.doi.org/10.1038/srep09038. Publicado • 10.1038/srep09038 Igreja, S.; Clarke, L. A.; Botelho, H. M.; Marques, L.; Amaral, M. D.. "Correction of a cystic fibrosis splicing mutation by antisense oligonucleotides". Human Mutation (2015): Carvalho, Sofia B.; Botelho, Hugo M; Leal, Sónia S.; Cardoso, Isabel; Fritz, Günter; Gomes, Cláudio M.. "Intrinsically Disordered and Aggregation Prone Regions Underlie ß-Aggregation in S100 Proteins". PLoS ONE 8 10 (2013): e76629. http://dx.doi.org/10.1371/journal.pone.0076629. Publicado • 10.1371/journal.pone.0076629 Botelho, Hugo M; Dahimène, S.; Uliyakina, I.; Neumann, B.; Tischer, C.; Pepperkok, R.; Amaral, M.D.. "WS20.6 High throughput screening as a source for novel cystic fibrosis therapeutic targets". Journal of Cystic Fibrosis 12 (2013): S42. http://dx.doi.org/10.1016/s1569-1993(13)60128-9. 10.1016/s1569-1993(13)60128-9 Dahimène, S.; Romão, A.; Botelho, Hugo M; Palma, M.; Correia, A.R.; Farinha, C.M.; King-Underwood, J.; et al. "WS21.9 Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays". Journal of Cystic Fibrosis 12 (2013): S45. http://dx.doi.org/10.1016/s1569-1993(13)60137-x. Publicado • 10.1016/s1569-1993(13)60137-x Sá-Moura, Bebiana; Simões, Ana Marisa; Fraga, Joana; Fernandes, Humberto; Abreu, Isabel A.; Botelho, Hugo M; Gomes, Cláudio M.; et al. "Biochemical and biophysical characterization of recombinant yeast proteasome maturation factor Ump1". Computational and Structural Biotechnology Journal 7 8 (2013): e201304006. http://dx.doi.org/10.5936/csbj.201304006. Publicado • 10.5936/csbj.201304006 Veith, A.; Botelho, Hugo M; Kindinger, F.; Gomes, C.M.; Kletzin, A.. "The sulfur oxygenase reductase from the mesophilic bacterium Halothiobacillus neapolitanus is a highly active thermozyme". Journal of Bacteriology 194 3 (2012): 677-685. http://www.scopus.com/inward/record.url?eid=2-s2.0-84855915891&partnerID=MN8TOARS. Publicado • 10.1128/JB.06531-11 Leal, S.S.; Botelho, Hugo M; Gomes, C.M.. "Metal ions as modulators of protein conformation and misfolding in neurodegeneration". Coordination Chemistry Reviews 256 19-20 (2012): 2253-2270. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864548981&partnerID=MN8TOARS. Publicado • 10.1016/j.ccr.2012.04.004 Botelho, Hugo M; Leal, S.S.; Cardoso, I.; Yanamandra, K.; Morozova-Roche, L.A.; Fritz, G.; Gomes, C.M.. "S100A6 amyloid fibril formation is calcium-modulated and enhances superoxide dismutase-1 (SOD1) aggregation". Journal of Biological Chemistry 287 50 (2012): 42233-42242. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871281215&partnerID=MN8TOARS. Publicado • 10.1074/jbc.M112.396416 Botelho, Hugo M; Gomes, C.M.. "Structural reorganization renders enhanced metalloprotein stability". Chemical Communications 47 39 (2011): 11149-11151. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053318341&partnerID=MN8TOARS. Publicado • 10.1039/c1cc13354c Fritz, G.; Botelho, Hugo M; Morozova-Roche, L.A.; Gomes, C.M.. "Natural and amyloid self-assembly of S100 proteins: Structural basis of functional diversity". FEBS Journal 277 22 (2010): 4578-4590. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049277867&partnerID=MN8TOARS. Publicado • 10.1111/j.1742-4658.2010.07887.x Botelho, Hugo M; Leal, S.S.; Veith, A.; Prosinecki, V.; Bauer, C.; Fröhlich, R.; Kletzin, A.; Gomes, C.M.. "Role of a novel disulfide bridge within the all-beta fold of soluble Rieske proteins". Journal of Biological Inorganic Chemistry 15 2 (2010): 271-281. http://www.scopus.com/inward/record.url?eid=2-s2.0-77949270785&partnerID=MN8TOARS. Publicado • 10.1007/s00775-009-0596-3 Botelho, Hugo M; Koch, M.; Fritz, G.; Gomes, C.M.. "Metal ions modulate the folding and stability of the tumor suppressor protein S100A2". FEBS Journal 276 6 (2009): 1776-1786. http://www.scopus.com/inward/record.url?eid=2-s2.0-61349168402&partnerID=MN8TOARS. Publicado • 10.1111/j.1742-4658.2009.06912.x Prosinecki, V.; Botelho, Hugo M; Francese, S.; Mastrobuoni, G.; Moneti, G.; Urich, T.; Kletzin, A.; Gomes, C.M.. "A proteomic approach toward the selection of proteins with enhanced intrinsic conformational stability". Journal of Proteome Research 5 10 (2006): 2720-2726. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750201140&partnerID=MN8TOARS. 10.1021/pr0602491
Capítulo de livro	Amaral, Margarida; Clarke, Luka A; Carlos M. Farinha; Botelho, Hugo. "Systems Biology and the New Omics". In Hodson and Geddes' Cystic Fibrosis, editado por Andrew Bush; Margarida D. Amaral; Jane C. Davies; Nicholas J. Simmonds; Jennifer L. Taylor-Cousar; Sarath C. Ranganathan, 96-110. Boca Raton, FL, Estados Unidos: Taylor & Francis. CRC Press, 2023. Publicado • 10.1201/9781003262763 Carvalho, Sofia B.; Cardoso, Isabel; Botelho, Hugo M; Yanamandra, Kiran; Fritz, Günter; Gomes, Cláudio M.; Morozova-Roche, Ludmilla A.. "Structural Heterogeneity and Bioimaging of S100 Amyloid Assemblies". In Bio-nanoimaging, 197-212. Elsevier, 2014. Publicado • 10.1016/b978-0-12-394431-3.00018-3 Botelho, Hugo M; Fritz, Günter; Gomes, Cláudio M.. "Analysis of S100 Oligomers and Amyloids". In Methods in Molecular Biology, 373-386. Humana Press, 2012. 10.1007/978-1-61779-551-0_25
Poster em conferência	Daniela Neugebauer; Mariana Caleiro; Vasco Cachatra; Moiteiro, Cristina; Amaral, Margarida; Botelho, Hugo M. "High content screening for the characterization of the GRK5-dependent p.Phe508del-CFTR rescue in cystic fibrosis". Trabalho apresentado em Spanish & Portuguese Advanced Optical Microscopy Meeting – SPAOM2023, 2023. Daniela Neugebauer; Vasco Cachatra; Moiteiro, Cristina; Amaral, Margarida; Botelho, Hugo M. "Identification of genes that restore p.Phe508del-CFTR traffic in cystic fibrosis models through GRK5 inhibition". Trabalho apresentado em Chem&BioChem – Postgraduate Students Meeting, DQB/FCUL, 2023. Marques, Rute; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Cyclin D1-CDK4/6 and PI3K signalling pathways as targets to combat metastatic triple negative and luminal A breast cancer". Trabalho apresentado em Jornadas Científicas 2023 Universidade de Lisboa, 2023. Marques, Rute; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Exploiting cyclin D1/CDK4/6 and/or PI3K pathways to fight metastatic triple negative and luminal A breast cancer". Trabalho apresentado em 14th iMed.ULisboa Postgraduate Students Meeting, 2023. Fernandes AA; Martinho, Paulo N.; Bento, Marcos; Botelho, Hugo. "Thermo- and Photo-responsive Spin Labile Fe(III) complexes". Trabalho apresentado em CQE Days 2023, 2023. Pinto S; DUARTE, Ana Patrícia; Botelho, Hugo; Gaspar, Maria Manuela; Ascenção L; Faísca P; Catarina Pinto Reis; Pacheco, Rita. "Albumin Nanoparticles of Natural Products: In Vitro and In Vivo Evaluation". Trabalho apresentado em 4th SPLC-CRS Young Scientists Meeting, 2023. Coelho M; DUARTE, Ana Patrícia; Botelho, Hugo; Catarina Pinto Reis; Maria Luísa Serralheiro; Pacheco, Rita. "Edible seaweeds extracts characterization and prospective health effects". Trabalho apresentado em 1st International Congress on Food, Nutrition & Public Health, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo; Malhó, Rui; Brito, Alexandra. "Detalhando as alterações fenotípicas e morfológicas subjacentes ao extravasamento das células de cancro da mama através da barreira hematoencefálica". Trabalho apresentado em 4º Encontro Nacional de Jovens Investigadores em Oncologia, 2022. M. A. Bento; Paulo Nuno Martinho; Botelho, Hugo M; Diogo, Hermínio P.; Liliana P. Ferreira; Vítor H. N. Rodrigues. Autor correspondente: M. A. Bento. "Fe(III) complexes as molecular magnetic actuators". Trabalho apresentado em MagnetismIn, 2022. Filipa S. Carvalho; Vukosava M Torres; Joana V. Ribeiro; Botelho, Hugo; Vilarinho, Laura; Cláudio M. Gomes; Henriques, Bárbara J. "Mitochondria phenotype and metabolomic signature in MADD patient-derived fibroblasts". Trabalho apresentado em SSIEM 2022 Annual Symposium, 2022. Marcos A. Bento; Botelho, Hugo; Ferreira, L.P.; Martinho, Paulo N.. "Molecular magnetic actuators based on Fe(III) complexes". Trabalho apresentado em EuChemS Chemistry Congress, 2022. Carvalho FS; Ribeiro JV; Botelho, Hugo; Vilarinho L; Gomes CM; Henriques, BJ. "Cellular studies to evaluate mitochondria function on MADD, a rare metabolic disorder". Trabalho apresentado em The Biochemistry Global Summit (25th IUBMB Congress, 46th FEBS Congress and 15th PABMB Congress), 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Dissecting the extravasation of triple-negative breast cancer cells across the blood-brain barrier: the landscape for brain metastasis prevention". Trabalho apresentado em 13th iMed.ULisboa Postgraduate Students Meeting, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Molecular characterization and modulation of blood-brain barrier and breast cancer cells interaction: a step closer to brain metastasis prevention". Trabalho apresentado em EACR 2022 Congress ¿ Innovative Cancer Science: Translating Biology to Medicine, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Understanding the blood-brain barrier and breast cancer cells' interaction: a step closer to brain metastasis prevention". Trabalho apresentado em Brain Tumor Meeting 2022, 2022. Godinho-Pereira, Joana; Lopes, Margarida Dionísio; Ana Rita Garcia; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Tackling breast cancer brain metastasis: a drug screening for a preventive approach to boost blood-brain barrier properties". Trabalho apresentado em Brain Tumor Meeting 2022, 2022. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Brito, Maria Alexandra. "Emerging events in breast cancer cells extravasation through blood-brain barrier: the key for brain metastasis prevention". Trabalho apresentado em Encontro Ciência 2022, 2022. Ferreira, JF; Silva, Iris AL; Botelho, Hugo M; Amaral, Margarida; Farinha, CM. "Exploring the role of EPAC1 to stabilize CFTR in intestinal cells". Trabalho apresentado em 17th ECFS Basic Science Conference, 2022. Botelho, Hugo M; Lopes-Pacheco, Miquéias; Pinto, Madalena C.; Silva, Iris AL; Clarke, Luka; Cachatra, V; Neumann, B; et al. "New kinase therapeutic targets for Cystic Fibrosis from a global functional genomics screen". Trabalho apresentado em 17th ECFS Basic Science Conference, 2022. Botelho, Hugo M; Lopes-Pacheco, Miquéias; Pinto, Madalena C.; Silva, Iris AL; Clarke, Luka A; Cachatra, V; Neumann, B; et al. "New kinase therapeutic targets for Cystic Fibrosis identified through high-content functional genomics screens". Trabalho apresentado em 5ª Conferência Anual da redeSAÚDE, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Brito, MA. "Spatio-temporal and molecular characterization of breast cancer cells extravasation through blood-brain-barrier". Trabalho apresentado em iMed.ULisboa Meeting 2021, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Disclosing molecular alterations in the blood-brain barrier endothelium during interaction with breast cancer cells: a step forward brain metastasis prevention.". Trabalho apresentado em Encontro de Ciência 2021, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Brito, MA. "Unravelling the extravasation of breast cancer cells through blood-brain barrier endothelial cells". Trabalho apresentado em 27th Porto Cancer Meeting, 2021. Ana Rita Garcia; Godinho-Pereira, Joana; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Unravelling the extravasation of breast cancer cells though blood-brain barrier endothelial cells.". Trabalho apresentado em Porto Cancer Meeting 2021, 2021. Dionisio Lopes, Margarida; Godinho-Pereira, Joana; Ana Rita Garcia; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "High-throughput screening of new and old drugs: a modulator to prevent breast cancer brain metastasis". Trabalho apresentado em Aims Meeting, 2021. Ana Rita Garcia; Joana Godinho-Pereira; Ines Figueira; KW Kim; Botelho, Hugo M; Rui Malhó; Maria Alexandra Brito. "Desvendando a interação entre as células de cancro da mama e as células endoteliais da barreira hematoencefálica ao longo do extravasamento". Trabalho apresentado em 2º Encontro Nacional de Jovens Investigadores em Oncologia, 2020. Garcia, R; Godinho-Pereira, J; Figueira, Inês; Kim, KS; Botelho, Hugo M; Malhó, R; Brito, MA. "Breast cancer cells extravasation into the brain: a dynamic trafficking across blood-brain barrier endothelium". Trabalho apresentado em 5th Annual Meeting of the Mind-Brain College of the University of Lisbon, 2019. Ramalho, AS; Vonk, AM; Silva, Iris AL; Botelho, Hugo M; Bor, R; van Mourik, P; Michel, S; et al. "High reproducibility of forskolin-induced swelling of intestinal organoids across three academic laboratories". Trabalho apresentado em 33rd Annual North American Cystic Fibrosis Conference, 2019. Garcia, R; Godinho-Pereira, J; Figueira, Inês; Cascão, R; Barata, J; Kim, KS; Marques, Luis; et al. "Interplay between breast cancer cells and blood-brain barrier endothelial cells along extravasation". Trabalho apresentado em 11th iMed.ULisboa Postgraduate Students Meeting and 4th i3DU Meeting, 2019. van Mourik, P; Vonk AM; Ramalho AS; Silva IAL; Botelho, Hugo; Bor R; Aguilera B; et al. "High reproducibility of forskolin-induced swelling of intestinal organoids across three academic laboratories". Trabalho apresentado em 42nd European Cystic Fibrosis Conference, 2019. Vonk, Annelotte M; Ramalho, Anabela S; Silva, Iris AL; Silva, Iris AL; Botelho, Hugo M; van Mourik, Peter; Michel, S; et al. "High reproducibility of forskolin-induced swelling of organoids across three academic laboratories.". Trabalho apresentado em 16th ECFS Basic Science Conference 2019, Dubrovnik, Croatia, 27-30 March 2019., 2019. Botelho, Hugo M; Halavatyi, A; Tischer, C. "shinyHTM: an open-source web application for the analysis of high throughput microscopy data". Trabalho apresentado em EMBL Conference: From Images to Knowledge with ImageJ & Friends (I2K), 2018. Astapenko, D; Botelho, Hugo M; Kolodkin, A; Westerhoff, HV; Amaral, Margarida. "Dynamic modelling of CFTR channel maturation in Cystic Fibrosis: what controls the channel concentration in the plasma membrane?". Trabalho apresentado em The 11th International Conference on Bioinformatics of Genome Regulation and Structure\Systems Biology, 2018. Quaresma, MC; Botelho, Hugo M; Pankonien, I; Pepperkok, R; Amaral, Margarida. "Role of CFTR in epithelial-mesenchymal transition (EMT) by functional genomics". Trabalho apresentado em Encontro Ciência 2018, 2018. Lopes-Pacheco, M; Botelho, Hugo M; Amaral, Margarida. "Novel F508del-CFTR traffic correctors among FDA-approved drugs and mechanism of action of RDR01752 corrector". Trabalho apresentado em EMBO Workshop: Proteostasis, 2017. Botelho, Hugo M; Lim, SH; Tischer, C; Pepperkok, R; Stagljar, I; Amaral, Margarida. "Validation and classification of genes releasing misfolded F508del-CFTR from the endoplasmic reticulum". Trabalho apresentado em EMBO Workshop: Proteostasis, 2017. Awatade, NT; Felício, V; Ramalho, S; Botelho, Hugo M; Silva, Iris AL; de Poel, E; Vonk, AM; et al. "Functional and biochemical assessment of correctors and potentiators on the rare Cystic Fibrosis-causing R560S mutation". Trabalho apresentado em 31st Annual North American Cystic Fibrosis Conference, 2017. Pinto, MC; Lérias, JR; Botelho, Hugo M; Awatade, NT; Pepperkok, R; Kunzelmann, K; Amaral, Margarida. "Anoctamin 1 – Identification of novel regulators and their significance to Cystic Fibrosis". Trabalho apresentado em 31st Annual North American Cystic Fibrosis Conference, 2017. Cristóvão, JS; Morris, V; Cardoso, Isabel; Leal, SS; Botelho, Hugo M; Göbl, C; Kierdorf, K; et al. "The neuronal S100B cytokine targeting amyloid-ß aggregation in Alzheimer’s Disease". Trabalho apresentado em 42nd FEBS Congress, 2017. Canato, S; Carvalho, AS; Botelho, Hugo M; Aloria, K; Matthiesen, Rune; Amaral, Margarida; Falcão, AO; Farinha, CM. "Modulation of protein traffic networks to rescue F508del-CFTR from the endoplasmic reticulum". Trabalho apresentado em 40th European Cystic Fibrosis Conference, 2017. Baptista, C; Botelho, Hugo M; Tenreiro, RP; Awatade, NT; Amaral, Margarida; Vieira, H. "From Portuguese natural heritage to Cystic Fibrosis therapies – a story of nature and technology for pharmaceutical applications". Trabalho apresentado em 3rd Annual Conference Rede Mar, 2017. Cristóvão, J; Morris, V; Cardoso, Isabel; Leal, SS; Botelho, Hugo M; Göbl, C; Kierdorf, K; et al. "Role of the S100B pro-inflammatory cytokine implicated in aging and Alzheimer’s Disease as a calcium-regulated chaperone acting on amyloid-ß aggregation". Trabalho apresentado em 4th Annual Conference Rede Saúde, 2017. Quaresma, MC; Botelho, Hugo M; Amaral, Margarida. "Role of CFTR in the maintenance of airway epithelial cell differentiation". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Pinto, MC; Lérias, JR; Botelho, Hugo M; Awatade, NT; Pepperkok, P; Kunzelmann, K; Amaral, Margarida. "Anoctamins 1 and 6 – Identification of novel traffic regulators and their significance to Cystic Fibrosis". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Lérias, JR; Pinto, MC; Botelho, Hugo M; Schreiber, R; Amaral, Margarida; Kunzelmann, K. "Anoctamin 1 traffic and function is enhanced by tethering proteins family of extended synaptotagmins". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Igreja, S; Botelho, Hugo M; Felício, V; Pagani, G; Pagani, M; Amaral, Margarida. "Validation of a CFTR alternative splicing reporter system for high-throughput microscopy and identification of novel CFTR regulators of alternative splicing". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Silva, Iris AL; Marques, Luis; Ramos, M; Amaral, Margarida. "CFTR modulators enhance function R334W-CFTR both in intestinal organoids and conditionally reprogrammed human nasal epithelial cells". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Felício, V; Botelho, Hugo M; Igreja, S; Amaral, Margarida. "A high-throughput screening assay to identify factors correcting CFTR mutations bearing premature termination codons (PTCs)". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Baptista, C; Vieira, H; Tenreiro, R; Awatade, NT; Amaral, Margarida; Botelho, Hugo M. "High throughput microscopy screens identify F508del-CFTR correctors and ANO1 traffic modulators in a unique Portuguese natural compound library". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Botelho, Hugo M; Lim, SH; Tischer, C; Pepperkok, R; Stagljar, I; Amaral, Margarida. "A high content siRNA screen at near genomic scale reveals novel F508del-CFTR therapeutic targets". Trabalho apresentado em 14th ECFS Basic Science Conference, 2017. Botelho, Hugo M; Awatade, NT; Tischer, C; Amaral, MD. "Modular high content image analysis workflows leveraging biology and biomedicine". Trabalho apresentado em NEUBIAS2020 BioImage Analysis Symposium, 2017. Botelho, Hugo M; Quaresma, MC; Amaral, Margarida. "CFTR in the maintenance of epithelial differentiation in the airways". Trabalho apresentado em Chem&BioChem Postgraduate Students Meeting, 2017. Igreja, S; Botelho, Hugo M; Amaral, Margarida. "Identification of novel regulators of alternative splicing by HT microscopy: implications for Cystic Fibrosis and other human diseases". Trabalho apresentado em EMBO Practical Course: High-Throughput Microscopy for Systems Biology, 2016. Pankonien, I; Santos-Sousa, L; Botelho, Hugo M; Amaral, Margarida. "Establishment of cell-based assays of airway epithelial differentiation and wound healing for automated microscopy". Trabalho apresentado em 13th ECFS Basic Science Conference, 2016. Lérias, JR; Afonso, Sara; Quaresma, MC; Pinto, M; Awatade, NT; Botelho, Hugo M; Schreiber, R; et al. "Anoctamin 1 - A member of a novel family of ion channels with extended functions and significance in disease". Trabalho apresentado em 13th ECFS Basic Science Conference, 2016. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Ramos, M; Amaral, Margarida. "To assess the efficacy of CFTR modulators on rare Cystic Fibrosis mutations using forskolin-induced swelling assay in human intestinal organoids". Trabalho apresentado em 13th ECFS Basic Science Conference, 2016. Awatade, NT; Botelho, Hugo M; Felício, V; Clarke, LA; Ramos, M; Amaral, Margarida. "Assessment of CFTR activity ex vivo as a basis for personalised medicine". Trabalho apresentado em 5th Workshop Structure and Function of Ion Channels and Transporters, 2015. Pankonien, I; Afonso, Sara; Quaresma, MC; Kmit, A; Dahimène, S; Botelho, Hugo M. "The epithelial transcription factor KLF4 affects the traffic of CFTR". Trabalho apresentado em 12th ECFS Basic Science Conference, 2015. Igreja, S; Botelho, Hugo M; Marques, Luis; Amaral, Margarida. "In vitro correction of the CFTR 2657+5G>A splicing mutation by an antisense oligonucleotide". Trabalho apresentado em 12th ECFS Basic Science Conference, 2015. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Awatade, NT; Proença, MC; Neumann, B; Tischer, C; et al. "Rescuing F508del-CFTR in airway epithelial cells using a novel traffic-based high content microscopy screening platform". Trabalho apresentado em 12th ECFS Basic Science Conference, 2015. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "A high content screening approach for the identification of CFTR traffic regulators in airway epithelial cells". Trabalho apresentado em ESF/EMBO Cell Polarity and Membrane Traffickin, 2014. Carvalho, SB; Botelho, Hugo M; Leal, SS; Cardoso, Isabel; Fritz, G; Gomes, Cláudio M. "Intrinsically disordered and aggregation prone regions underlie ß-aggregation in S100 proteins". Trabalho apresentado em 2nd Iberian Congress on Prions, 2013. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "High throughput screen reveals genes regulating wt-CFTR and rescuing F508del-CFTR traffic". Trabalho apresentado em 27th Annual North American Cystic Fibrosis Conference, 2013. Botelho, Hugo M; Dahimène, S; Uliyakina, I; Neumann, B; Tischer, C; Pepperkok, R; Amaral, Margarida. "Identification of CFTR traffic regulators as a source of novel Cystic Fibrosis therapeutic targets". Trabalho apresentado em The 5th EMBO Meeting, 2013. Sofia B Carvalho; Sónia S. Leal; Botelho, Hugo M; Isabel Cardoso; Günter Fritz; Cláudio M. Gomes. "Cross talks between amyloid-forming proteins in neurodegeneration". Trabalho apresentado em 9th EBSA European Biophysics Congress, 2013. Botelho, Hugo; Dahimène S; Uliyakina I; Neumann B; Tischer C; Pepperkok R; Amaral MD. "High throughput screening as a source for novel cystic fibrosis therapeutic targets". Trabalho apresentado em 36th European Cystic Fibrosis Conference, 2013. Dahimène S; Romão A; Botelho, Hugo; Palma M; Correia AR; Farinha CM; King-Underwood J; et al. "WS21.9 Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays". Trabalho apresentado em 36th European Cystic Fibrosis Conference, 2013. Bandeiras, T; Freitas, MC; Petrasch, D; Botelho, Hugo M; Gomes, Cláudio M; Kletzin, A; Frazão, Carlos. "Purification, crystallization and preliminary crystallographic analysis of Acidianus ambivalens Rieske ferredoxin 2 (RFd2), a new type of Rieske ferredoxins with an additional disulfide". Trabalho apresentado em 1° Encontro Nacional de Utilizadores de Radiação de Sincrotrão, 2012. Botelho, Hugo M; Christine Betz; Katrin Kierdorf; Marco Prinz; Cláudio M. Gomes; Günter Fritz. "A new emerging role of S100B in amyloid plaque formation". Trabalho apresentado em 56th Annual Meeting, German Society for Neuropathology and Neuroanatomy, 2011. 10.5414/npp30245 Bandeiras, T; Freitas, MC; Botelho, Hugo M; Gomes, Cláudio M; Carrondo, MA; Kletzin, A; Frazão, Carlos. "Crystallographic structure of Acidianus ambivalens Rieske ferredoxin 2 – The disulfide bridge structural role". Trabalho apresentado em EMBO Practical Course: High-throughput methods for Protein Production and Crystallization, 2011. Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA; Gomes, Cláudio M. "S100 proteins: modulators of Aß amyloidogenesis". Trabalho apresentado em Interbio Symposium 'Frontiers in Protein Research', 2011. Gomes, Cláudio M; Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA. "Metal ions and amyloid-beta peptide fibrils as modulators of S100 amyloidogenesis". Trabalho apresentado em FASEB Summer Research Conference - Protein Folding in the Cell, 2010. Botelho, Hugo M; Yanamandra, K; Fritz, G; Morozova-Roche, LA; Gomes, Cláudio M. "Metal ions and Aß fibrils modulate S100 amyloidogenesis". Trabalho apresentado em IV Spanish-Portuguese Biophysics Congress, 2010. Almeida, M; Magalhães, LC; Sabino, FMR; Botelho, Hugo M; Gomes, Cláudio M. "Focused NMR structural proteomics on hyperstable proteome subsets". Trabalho apresentado em EUROMAR 2010, 2010. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Metal ions as modulators of folding and stability of the S100A2 tumor suppressor". Trabalho apresentado em Gordon Research Seminar & Gordon Research Conference “Protein Folding Dynamics”, 2010. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Calcium and zinc modulate the folding and stability of the S100A2 tumor suppressor". Trabalho apresentado em VIII European Symposium of The Protein Society, 2009. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Ca2+ and Zn2+ modulate the conformation and stability of the S100A2 tumor suppressor". Trabalho apresentado em The 9th European Biological Inorganic Chemistry Conference, EUROBIC9, 2008. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Role of Ca2+ and Zn2+ as modulators of the S100A2 tumor suppressor". Trabalho apresentado em FASEB Summer Research Conference – Protein Folding in the Cell, 2008. Nascimento, C; Botelho, Hugo M; Tavares de Almeida, I; Gomes, Cláudio M; Leandro P. "Folding rescue of mild PKU mutations by chemical chaperones". Trabalho apresentado em Society for the Study of Inborn Errors of Metabolism Symposium 2007, SSIEM Symposium 2007, 2007. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Conformational control of S100A2 by metal ions". Trabalho apresentado em 13th International conference on Biological Inorganic Chemistry, ICBIC XIII, 2007. Prosinecki, V; Botelho, Hugo M; Gomes, Cláudio M. "A proteomic approach to investigate protein stability". Trabalho apresentado em VII European Symposium of the Protein Society – From Proteins to Proteome, 2007. Botelho, Hugo M; Koch, M; Fritz, G; Gomes, Cláudio M. "Studies on the conformational effects of calcium and zinc binding to S100A2". Trabalho apresentado em VII European Symposium of the Protein Society – From Proteins to Proteome, 2007. Prosinecki, V; Botelho, Hugo M; Francese, S; Mastrobuoni, G; Moneti, G; Urich, T; Kletzin, A; Gomes, Cláudio M. "Mining for proteins with enhanced intrinsic thermal stability: a proteomic research on hyperthermophile Sulfurisphaera sp.". Trabalho apresentado em International Conference on Proteomics, PROTEOMLUX, 2006. Francese, S; Prosinecki, V; Botelho, Hugo M; Mastrobuoni, G; Pieraccini, G; Kletzin, A; Gomes, Cláudio M. "Proteomic characterization of hyperstable proteins within the soluble proteome of Sulfurisphaera sp.". Trabalho apresentado em 17th International Mass spectrometry Conference, 2006. Prosinecki, V; Botelho, Hugo M; Francese, S; Kletzin, A; Gomes, Cláudio M. "Ferredoxin and oxidative stress defence proteins are among the most stable proteins in the thermophile Sulfurisphaera sp.: a proteomic study". Trabalho apresentado em EUROBIC Young Researchers Forum and The 8th European Biological Chemistry Conference, EUROBIC8, 2006.
Resumo em conferência	Marques, Rute S.; Ana Rita Garcia; Godinho-Pereira, Joana; Malhó, Rui; Botelho, Hugo M; Brito, Maria. "Targeting Cyclin D1/CDK4/6 and PI3K pathways for the treatment of metastatic breast cancer.". Trabalho apresentado em Fundação AstraZeneca Innovate Competition, iMed Conference® Lisbon 2023, Lisbon, 2023. Submetido Dionisio Lopes, Margarida; Godinho-Pereira, Joana; Figueira, Inês; Botelho, Hugo M; Malhó, Rui; Maria Alexandra de Oliveira Silva Braga Pedreira de Brito. "Screening de novos e antigos fármacos: um modulador para a prevenção de metástases ccerebrais do cancro da mama.". Trabalho apresentado em 2º Encontro Nacional de Jovens Investigadores em Oncologia, Porto, 2020.

Propriedade Intelectual

Patente	Margarida D. Amaral; Botelho, Hugo M; Lopes-Pacheco, Miquéias. 2023. "Method of identifying agents for the treatment of cystic fibrosis caused by the mutation F508del". Concedida/Emitida
Pedido provisório de patente	2022. "Method of identifying agents for the treatment of cystic fibrosis caused by the mutation F508del". Protegido

Atividades

Apresentação oral de trabalho

	Título da apresentação	Nome do evento Anfitrião (Local do evento)
2023/10/25	High content screening methods and algorithms for basic and applied research on Cystic Fibrosis	SPAOM2023 - Spanish & Portuguese Advanced Optical Microscopy Meeting PPBI & REMOA (Faro, Portugal)
2023/06/10	Global functional genomics reveals GRK5 as a therapeutic target for cystic fibrosis	46th ECFS Conference European Cystic Fibrosis Society (Vienna, Áustria)
2023/05/12	High content screening from a core facility perspective: from experiments to discoveries	5th NEUBIAS Symposium Network of European BioImage Analysts (Porto, Portugal)
2022/04/01	New kinase therapeutic targets for Cystic Fibrosis from a global functional genomics screen	17th ECFS Basic Science Conference European Cystic Fibrosis Conference (Albufeira, Portugal)
2022	37 lectures and workshops on Cystic Fibrosis, Microscopy and Bioimage Analysis at national and international events.
2021/11/03	Functional genomics reveals global genes rescuing F508del-CFTR traffic and novel kinase therapeutic targets complementing current correctors	35th North American Cystic Fibrosis Conference Cystic Fibrosis Foundation (Estados Unidos)
2021/02/04	High-throughput microscopy and screening	Optical microscopy at COLife and Friends: learning the basics COLife (Lisboa, Portugal)
2020/11/26	VALHealth – Valorisation of ALgae for Health: Bioactive compounds from marine bioresources by membrane technology	BioISI Research Day 2020 BioISI (Lisboa, Portugal)
2020/11/24	High content image analysis with CellProfiler	SPAOM2020: Spanish Portuguese Meeting for Advanced Optical Microscopy REMOA / PPBI (Valencia, Espanha)
2020/09/24	Screening de novos e antigos fármacos: um modulador para a prevenção de metástases cerebrais do cancro da mama	2º Encontro Nacional de Jovens Investigadores em Oncologia Liga Portuguesa Contra o Cancro (Portugal)
2020/06/30	PPBI - Plataforma Portuguesa de BioImagem	6º Fórum de Gestão de Dados de Investigação FCT-FCCN – Fundação para a Ciência e Tecnologia (Portugal)
2019/11/13	Breast cancer cells extravasation into the brain: a dynamic trafficking across blood-brain barrier endothelium	5th Annual Meeting of the Mind-Brain College of the University of Lisbon University of Lisbon (Lisbon, Portugal)
2019/11/06	Analysis of time lapse microscopy data with CellProfiler et al.	3rd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2019 Red Española de Microscopía Óptica Avanzada & Portuguese Platform of BioImaging (Coimbra, Portugal)
2019/10/01	A systems view of Cystic Fibrosis: modeling the folding and maturation pathways of the CFTR protein	2nd CORBEL Service Operator and User Meeting CORBEL (Heidelberg, Alemanha)
2018/12/06	shinyHTM: an open-source web application for the analysis of high throughput microscopy data	EMBL Conference: From Images to Knowledge with ImageJ & Friends (I2K) European Molecular Biology Laboratory (Heidelberg, Alemanha)
2018/10/26	Innovating the search for novel Cystic Fibrosis therapies using high content microscopy screening	SPAOM2018: Spanish Portuguese Meeting for Advanced Optical Microscopy Red Española de Microscopía Óptica Avanzada & Portuguese Platform of BioImaging (Granada, Espanha)
2018/01/22	A systems view of Cystic Fibrosis: modeling the folding and maturation pathways of the CFTR protein	CORBEL Work Package 4 Service Operator meeting CORBEL (Berlin, Alemanha)
2017/06/09	CFTR traffic – Functional genomics screens to identify novel regulators	40th European Cystic Fibrosis Conference European Cystic Fibrosis Society (Seville, Espanha)
2017/04/01	A high-content siRNA screen at near genomic scale reveals novel F508del-CFTR therapeutic targets	14th ECFS Basic Science Conference European Cystic Fibrosis Society (Albufeira, Portugal)
2017/02/15	Modular high content image analysis workflows leveraging biology and biomedicine	NEUBIAS2020 BioImage Analysis Symposium Network of European BioImage Analysts - NEUBIAS (Lisbon, Portugal)
2016/10/13	Innovating the search for novel Cystic Fibrosis therapies using high content microscopy screening	BioISI Post Grad Seminar Biosystems & Integrative Sciences Institute (Lisbon, Portugal)
2015/11/13	Assessment of CFTR activity ex vivo as a basis for personalised medicine	5th Structure and Function of Ionic Channels and Transporters Workshop Faculty of Pharmacy of the University of Lisbon (Lisbon, Portugal)
2015/06/02	A high throughput fluorescence microscopy pipeline for drug discovery in Cystic Fibrosis	1st Course on High Content Screening and Image Analysis for Biosciences i3S - Instituto de Investigação e Inovação da Universidade do Porto (Porto, Portugal)
2015/05/10	S100 proteins as novel metal-ion regulated molecular chaperones in neurodegeneration	EMBO Conference: Molecular chaperones: From molecules to cells and misfolding diseases European Molecular Biology Organization (Heraklion, Grécia)
2014/08/15	A high content screening system for the development of CFTR targeting therapies in the genetic disease Cystic Fibrosis	International ITU Molecular Biology and Genetics Students Congress ‘14 Istanbul Technical University (Istanbul, Turquia)
2014/03/26	A high-content siRNA screens identify novel CFTR traffic regulators in human airway epithelial cells	11th ECFS Basic Science Conference European Cystic Fibrosis Society (St. Julians, Malta)
2013/10/17	High throughput screen reveals genes regulating wt-CFTR and rescuing F508del-CFTR traffic	27th Annual North American Cystic Fibrosis Conference Cystic Fibrosis Foundation (Salt Lake City, Estados Unidos)
2013/06/14	High throughput screening as a source for novel Cystic Fibrosis therapeutic targets	36th European Cystic Fibrosis Society Conference European Cystic Fibrosis Society (Lisbon, Portugal)
2013/05/17	Cross talk between S100 proteins and amyloid-forming proteins in neurodegeneration	EMBO Conference: The biology of molecular chaperones: From molecules, organelles and cells to misfolding diseases European Molecular Biology Organization (Santa Margherita di Pula, Itália)
2013/05/14	Screening for novel F508del-CFTR correctors identified by pharmacophore modelling through automated microscopy and NBD1 folding assays	36th European Cystic Fibrosis Society Conference European Cystic Fibrosis Society (Lisbon, Portugal)
2013/03/07	High-throughput identification of CFTR traffic regulators	BioFIG PhD Student/Post-Doc Seminar Center for Biodiversity, Functional & Integrative Genomics (Lisbon, Portugal)
2011/01/19	Aß fibrils and metal ions modulate S100 amyloidogenesis: novel players in neurodegeneration?	Lisbon Area Neuroscience Meeting Instituto de Medicina Molecular (Lisbon, Portugal)
2010/07/08	Metal ions and Aß fibrils modulate S100 amyloidogenesis	IV Spanish Portuguese Biophysical Congress Sociedad de Biofísica de España, Portuguese Biophysical Society (Zaragoza, Espanha)
2009/11/04	Metal ions as modulators of folding and amyloidogenic pathways in the S100 protein family	ITQB PhD Seminar Instituto de Tecnologia Química e Biológica (Oeiras, Portugal)
2007/02/02	Modulation of the stability of S100A2 variants by metal ions	Workshop Proteins in Health and Disease: from structure to function Faculty of Pharmacy, University of Lisbon (Lisbon, Portugal)

Orientação

	Título / Tema Papel desempenhado	Curso (Tipo) Instituição / Organização
2023/09/04 - 2024/09/01	Characterizing the regulation of the CFTR protein by the kinase GRK5 in cystic fibrosis models Orientador	Bioquímica (Mestrado) Universidade de Lisboa Faculdade de Ciências, Portugal
2023/04/01 - 2023/10/01	Regulation of the intracellular traffic of p.Phe508del-CFTR by the kinase GRK5 in Cystic Fibrosis models Orientador de Daniela Neugebauer	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2022/09 - 2023/07	Cristais de spin lábil termo e foto-sensíveis para músculos artificiais Coorientador de André Fernandes	Química (Licenciatura/Bacharelato) Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09/20 - 2022/07/15	Trainee bioimage analyst at BioISI’s High-Throughput Screening Facility Orientador de Pau Rubio Costa	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2020/09/29 - 2022/01/31	Application of new bioactive compounds extracted from algae: health benefits and effects on Cystic Fibrosis Coorientador de Ana Patrícia Rodrigues Duarte	Engenharia Química e Biológica (Mestrado) Instituto Politécnico de Lisboa Instituto Superior de Engenharia de Lisboa, Portugal
2021/07/17 - 2021/12/31	Laboratory rotation, part of PhD course. Time-lapse confocal imaging of mixed 3D cell cultures. Optimization of a cellular extravasation assay Orientador de Ana Rita da Costa Garcia	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/03/05 - 2021/03/31	Bioimage analyst at BioISI High-Throughput Screening Facility Orientador de Aires Miguel Gomes Delgado de Almeida Duarte	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2020/12/03 - 2020/12/03	Laboratory rotation, part of PhD course. Confocal imaging of mixed 3D cell cultures; tools for 3D data visualization and cell tracking Orientador de Joana Godinho Pereira	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/09/06 - 2020/09/17	Effect of Novel CFTR Interactors on Traffic of F508del Mutant: Implications for Cystic Fibrosis Coorientador de Joana Cartaxo Serralha	Biomedical Science (Licenciatura/Bacharelato) King's College London Faculty of Life Sciences and Medicine, Reino Unido Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/02/01 - 2018/12/31	Deconvolution of dual CFTR/ANO1 Modulators from Portuguese natural products - A new class of drugs for CF therapy Orientador de Joana Godinho-Pereira	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2016/05/01 - 2017/12/31	The identification of new natural compounds of high therapeutic potential for Cystic Fibrosis by high-throughput microscopy screens Orientador de Catarina Duarte Galhardo Baptista	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2014 - 2015	Effect of TGFß signaling on CFTR protein: consequences for epithelial differentiation in CF Coorientador de Margarida Cuco Quaresma	Bioquímica (Mestrado) Universidade de Lisboa Faculdade de Ciências, Portugal
2010 - 2011	Molecular and mechanistic aspects of the effect of metal ions in the conformation and amyloidogenesis of the S100B protein Coorientador de Sofia Carvalho	Bioquímica (Mestrado) Universidade de Lisboa Faculdade de Ciências, Portugal

Organização de evento

	Nome do evento Tipo de evento (Tipo de participação)	Instituição / Organização
2024/04/15 - 2024/04/19	Advanced Course on the Principles of Light Microscopy (2024/04/15 - 2024/04/19) Oficina (workshop) (Membro da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/12/11 - 2023/12/13	Introduction to Image Analysis (2023/12/11 - 2023/12/13) Oficina (workshop) (Membro da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/03 - 2023/10/27	SPAOM2023 – Spanish-Portuguese Advanced Optical Microscopy 2023 (2023/10/25 - 2023/10/27) Congresso (Membro da Comissão Científica)
2023/08/28 - 2023/08/30	Training on confocal microscopy and core facility management (2023/08/28 - 2023/08/30) Oficina (workshop) (Presidente da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/03/20 - 2023/03/22	Macro Scripting in ImageJ - COLife & FCUL (2023/03/20 - 2023/03/22)
2022/12/13 - 2022/12/16	Basics in Light Microscopy (2022/12/13 - 2022/12/16) Oficina (workshop) (Presidente da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/09/21 - 2022/09/23	Introduction to Image Analysis - COLife & FCUL Course (2022/09/21 - 2022/09/23) Oficina (workshop) (Membro da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/07/15 - 2022/07/15	Chem&BioChem – Postgraduate Students Meeting, FCUL (2022/07/15 - 2022/07/15) Encontro (Membro da Comissão Científica)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/06/22 - 2022/06/22	Hands-on CellProfiler training for Lisbon Area Bioimaging Group members (2022/06/22 - 2022/06/22) Oficina (workshop) (Presidente da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/01/18 - 2022/01/20	Macro Scripting in ImageJ - COLife & FCUL (2022/01/18 - 2022/01/20) Oficina (workshop) (Coorganizador)
2021/11/23 - 2021/11/25	SPAOM2021 – Spanish-Portuguese Advanced Optical Microscopy 2021 (2021/11/23 - 2021/11/25) Congresso (Membro da Comissão Científica)	Portuguese Platform of BioImaging, Portugal
2021/10/25 - 2021/10/28	Basics in Light Microscopy (2021/10/25 - 2021/10/28) Oficina (workshop) (Presidente da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09/21 - 2021/09/23	Introduction to Image Analysis COLife & FCUL (2021/09/21 - 2021/09/23) Oficina (workshop) (Coorganizador)
2021/02/15 - 2021/02/17	Eutopia 3 - Third Meeting of the European Topology Interdisciplinary Initiative (2021/02/15 - 2021/02/17) Encontro (Membro da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2019/06/11 - 2019/06/12	Workshop on Integrative Approaches to Protein Folding & Aggregation (2019/06/11 - 2019/06/12) Oficina (workshop) (Membro da Comissão Organizadora)	Universidade de Lisboa Faculdade de Ciências, Portugal
2018/07/09 - 2018/07/13	Hands-on Workshop on High-Throughput Microscopy (2018/07/09 - 2018/07/13) Oficina (workshop) (Coorganizador)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/07/17 - 2017/07/21	Hands-on Workshop on High-Throughput Microscopy (2017/07/17 - 2017/07/21) Oficina (workshop) (Coorganizador)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2016/07/11 - 2016/07/15	Hands-on Workshop on High-Throughput Microscopy (2016/07/11 - 2016/07/15) Oficina (workshop) (Coorganizador)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2015/03/18 - 2015/03/20	Hands-on Workshop on High-Throughput Microscopy (2015/03/18 - 2015/03/20) Oficina (workshop) (Coorganizador)	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

Participação em evento

	Descrição da atividade Tipo de evento	Nome do evento Instituição / Organização
2022/03/30 - 2022/04/02	17th ECFS Basic Science Conference Congresso	17th European Cystic Fibrosis Society Basic Science Conference
2021/11/30 - 2021/11/30	5.ª Conferência Anual da redeSAÚDE Conferência	5.ª Conferência Anual da redeSAÚDE Universidade de Lisboa, Portugal
2021/11/23 - 2021/11/25	SPAOM2021 Congresso	Spanish & Portuguese Advanced Optical Microscopy Meeting
2021/11/02 - 2021/11/05	35th NACFC Congresso	35th North American Cystic Fibrosis Conference
2021/02/02 - 2021/02/04	Optical microscopy at COLife & Friends: learning the basics Oficina (workshop)	Optical microscopy at COLife and Friends: learning the basics
2020/11/24 - 2020/11/27	SPAOM2020 Congresso	Spanish & Portuguese Advanced Optical Microscopy Meeting
2020/09/24 - 2020/09/25	43rd ECFS Conference Congresso	43rd European Cystic Fibrosis Conference
2020/06/30 - 2020/07/01	6º Fórum GDI Conferência	6º Fórum de Gestão de Dados de Investigação Portuguese Platform of BioImaging, Portugal
2019/11/06 - 2019/11/08	SPAOM2019 Encontro	3rd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2019 Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/10/01 - 2019/10/02	2nd CORBEL Service Operator and User Meeting Encontro	2nd CORBEL Service Operator and User Meeting Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/06/19 - 2019/06/19	Preclinical Imaging Lifesciences Oficina (workshop)	Preclinical Imaging Lifesciences Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/12/05 - 2018/12/08	From images to knowledge with ImageJ & friends (I2K) Conferência	EMBL Conference: From images to knowledge with ImageJ & friends (I2K) and I2K Developers Satellite Meeting European Molecular Biology Laboratory, Alemanha
2018/10/23 - 2018/10/26	SPAOM2018 Encontro	2nd Spanish-Portuguese Meeting for Advanced Optical Microscopy – SPAOM2018 Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2018/02/22 - 2018/02/23	CORBEL WP4 Service Operator Meeting Encontro	CORBEL WP4 Service Operator Meeting Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/09/17 - 2017/09/21	EMBO Workshop Oficina (workshop)	EMBO Workshop – Proteostasis European Molecular Biology Organization, Alemanha
2017/06/07 - 2017/06/10	40th European Cystic Fibrosis Conference Conferência	40th European Cystic Fibrosis Conference European Cystic Fibrosis Society, Dinamarca
2017/06/06 - 2017/06/09	3rd Course on High Content Screening and Image Analysis for BioSciences Outro	3rd Course on High Content Screening and Image Analysis for BioSciences Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017/05/08 - 2017/05/08	3rd Annual Conference Rede Mar Conferência	3rd Annual Conference Rede Mar Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2017/03/29 - 2017/04/01	14th ECFS Basic Science Conference Conferência	14th ECFS Basic Science Conference European Cystic Fibrosis Society, Dinamarca
2017/02/15 - 2017/02/17	NEUBIAS2020 BioImage Analysis Symposium Simpósio	NEUBIAS2020 BioImage Analysis Symposium European Cooperation in Science and Technology, Bélgica
2016/07/04 - 2016/07/06	Ciência 2016 Encontro	Ciência 2016 - Science and Technology in Portugal Meeting Fundação para a Ciência e a Tecnologia, Portugal
2016/06/14 - 2016/06/17	2nd Course on High Content Screening and Image Analysis for Biosciences Outro	2nd Course on High Content Screening and Image Analysis for Biosciences Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/06/01 - 2015/06/04	1st Course on High Content Screening and Image Analysis for Biosciences Outro	1st Course on High Content Screening and Image Analysis for Biosciences Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/03/25 - 2015/03/28	12th ECFS Basic Science Conference Conferência	12th ECFS Basic Science Conference European Cystic Fibrosis Society, Dinamarca
2014/08/15 - 2014/08/18	ITU Molecular Biology and Genetics Students Congress Congresso	International ITU Molecular Biology and Genetics Students Congress ’14 Istanbul Teknik Üniversitesi, Turquia European Molecular Biology Organization, Alemanha
2014/05/10 - 2014/05/15	ESF/EMBO Cell Polarity and Membrane Trafficking Conferência	ESF/EMBO Cell Polarity and Membrane Trafficking European Molecular Biology Organization, Alemanha European Science Foundation, França
2013/10/17 - 2013/10/19	27th Annual NACFC Conferência	27th Annual North American Cystic Fibrosis Conference Cystic Fibrosis Foundation, Estados Unidos European Molecular Biology Organization, Alemanha
2013/09/21 - 2013/09/24	The 5th EMBO Meeting Encontro	The 5th EMBO Meeting European Molecular Biology Organization, Alemanha
2013/07/18 - 2013/07/18	Molecular Biology in Portugal and EMBL Encontro	Molecular Biology in Portugal and EMBL Universidade de Lisboa Faculdade de Ciências, Portugal
2013/06/12 - 2013/06/15	36th ECFS Conference Conferência	36th European Cystic Fibrosis Society Conference European Cystic Fibrosis Society, Dinamarca Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Alemanha
2011/10/26 - 2011/10/28	2nd ITQB PhD Students’ Meeting Encontro	2nd ITQB PhD Students’ Meeting Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Alemanha
2011/09/21 - 2011/09/24	56th Annual Meeting DGNN Encontro	56th Annual Meeting, German Society for Neuropathology and Neuroanatomy Deutschen Gesellschaft für Neuropathologie und Neuroanatomie, Alemanha
2011/05/05 - 2011/05/07	Interbio Symposium Simpósio	Interbio Symposium 'Frontiers in Protein Research' Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/10/21 - 2010/10/22	1st ITQB PhD Students' Meeting Encontro	1st ITQB PhD Students' Meeting Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/07/07 - 2010/07/10	IV Spanish-Portuguese Biophysical Congress Congresso	IV Spanish-Portuguese Biophysical Congress Sociedade Portuguesa de Biofísica, Portugal Sociedad de Biofísica de España, Espanha
2010/01/10 - 2010/01/15	Gordon Research Conference Conferência	Gordon Research Conference “Protein Folding Dynamics”
2010/01/09 - 2010/01/10	Gordon Research Seminar Seminário	Gordon Research Seminar “Protein Folding Dynamics”
2009/10/30 - 2009/11/01	8th Short Course of the Portuguese Biophysical Society Outro	8th Short Course of the Portuguese Biophysical Society “Systems Biology” Sociedade Portuguesa de Biofísica, Portugal
2009/06/14 - 2009/06/18	VIII European Symposium of The Protein Society Simpósio	VIII European Symposium of The Protein Society Protein Society, Estados Unidos
2009/03/19 - 2009/03/19	Protein Folding Diseases: From Metabolic Disorders to Neurodegeneration Oficina (workshop)	International Workshop “Protein Folding Diseases: From Metabolic Disorders to Neurodegeneration” Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2008/10/02 - 2008/10/03	Workshop Physics of Biological Systems Oficina (workshop)	Workshop Physics of Biological Systems: From Genes to Societies Universidade de Lisboa Centro de Física Teórica e Computacional, Portugal
2008/09/02 - 2008/09/06	EUROBIC9 Conferência	The 9th European Biological Inorganic Chemistry Conference, EUROBIC9 Society of Biological Inorganic Chemistry, Estados Unidos
2008/07/27 - 2008/08/01	FASEB Summer Research Conference Conferência	FASEB Summer Research Conference – Protein Folding in the Cell Federation of American Societies for Experimental Biology, Estados Unidos
2008/03/07 - 2008/03/07	1st Symposium iMed.UL Simpósio	1st Symposium iMed.UL Universidade de Lisboa Faculdade de Farmácia, Portugal
2007/07/15 - 2007/07/20	ICBIC XIII Conferência	13th International conference on Biological Inorganic Chemistry, ICBIC XIII Society of Biological Inorganic Chemistry, Estados Unidos
2007/05/12 - 2007/05/16	VII European Symposium of the Protein Society Simpósio	VII European Symposium of the Protein Society – From Proteins to Proteome Protein Society, Estados Unidos
2007/02/02 - 2007/02/03	Workshop Proteins in Health and Disease Oficina (workshop)	Workshop Proteins in Health and Disease: from Structure to Function Universidade de Lisboa Faculdade de Farmácia, Portugal
2006/11/08 - 2006/11/08	A one day workshop on protein science Oficina (workshop)	A one day workshop on protein science Universidade de Lisboa Centro de Física Teórica e Computacional, Portugal
2006/07/01 - 2006/07/06	EUROBIC8 Conferência	EUROBIC8 – 8th European Biological Inorganic Chemistry Conference and Young Researchers Forum Society of Biological Inorganic Chemistry, Estados Unidos

Júri de grau académico

	Tema Tipo de participação	Nome do candidato (Tipo de grau) Instituição / Organização
2022/03/21	Amyloids in Nature: structural analysis of sea urchin inspired adhesive protein Arguente principal	André Gonzaga Ribeiro de Castro Fernandes (Mestrado) Universidade de Lisboa Faculdade de Ciências, Portugal
2022/03/21	Amyloids in Nature: structural analysis of sea urchin inspired adhesive protein Arguente principal	André Gonzaga Ribeiro de Castro Fernandes (Mestrado) Universidade de Lisboa Faculdade de Ciências, Portugal

Arbitragem científica em revista

	Nome da revista (ISSN)	Editora
2023/05/02 - Atual	Healthcare (2227-9032)	MDPI
2023/03 - Atual	Cells (2073-4409)	MDPI
2023 - Atual	Journal of Microscopy (1365-2818)	Wiley
2023 - Atual	International Journal of Molecular Sciences (1422-0067)	MDPI
2022/11 - Atual	Medicina (1648-9144)	MDPI
2021 - Atual	Molecular Genetics & Genomic Medicine (2324-9269)	Wiley Periodicals, LLC
2021 - Atual	Journal of Visualized Experiments (1940-087X)
2018/11 - Atual	SLAS DISCOVERY Advancing the Science of Drug Discovery (2472-5560)	SAGE Publications
2016/11 - Atual	Scientific Reports (2045-2322)	Springer Science and Business Media LLC
2014/11 - Atual	PLoS ONE (1932-6203)	Public Library of Science
2013/08 - Atual	Biochimie (0300-9084)	Elsevier
2013/02 - Atual	Metallomics (1756-591X)	The Royal Society of Chemistry
2012/12 - Atual	FEBS Letters (1873-3468)	Wiley (John Wiley & Sons)
2012/12 - Atual	Journal of Dermatological Science (0923-1811)	Elsevier
2011 - Atual	Physical Chemistry Chemical Physics (1463-9076)	Royal Society of Chemistry
2011 - Atual	Journal of Biotechnology (0168-1656)	Elsevier

Comissão de avaliação

	Descrição da atividade Tipo de assessoria	Instituição / Organização	Entidade financiadora
2021/07/29 - Atual	Maria Joana Valente Ribeiro Main opponent: Seminário de Investigação I (1st year PhD discussion) Avaliador	Universidade de Lisboa Faculdade de Ciências, Portugal
2021 - Atual	Effective jury member for hiring an MSc student for CFF grant “Rep2CFTR: Repurposing FDA-Approved Drugs for Class II Rare CFTR Mutants”. PI: Miquéias Lopes-Pacheco Avaliador	Universidade de Lisboa Faculdade de Ciências, Portugal
2020/02/21 - Atual	Jury member for hiring a PhD researcher for FCT project “ProDysMito” Reference PTDC/BIA-BQM/29963/2017 PI: Bárbara J Henriques Avaliador	Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia

Curso / Disciplina lecionado

	Disciplina	Curso (Tipo)	Instituição / Organização
2024 - Atual	Experimental Biochemistry IV	Bioquímica (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2022/02 - 2024/07	Fundamentos de Química e Bioquímica [Fundamentals in Chemistry and Biochemistry]	Engenharia Biomédica e Biofísica (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/02 - 2023/07	Introdução à Biologia Molecular [Introduction to Molecular Biology]	Bioquímica (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2023/02 - 2023/07	Projeto [Project]	Química (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal
2020/02 - 2020/06	Biochemistry	Biologia (Licenciatura)	Universidade de Lisboa Faculdade de Ciências, Portugal

Membro de comissão

	Descrição da atividade Tipo de participação	Instituição / Organização
2023/12 - Atual	Chair of Image Analysis Working Group Coordenador	Portuguese Platform of BioImaging, Portugal
2022 - Atual	Member of PhD thesis advisory committee - Maria Joana Valente Ribeiro PhD in Biochemistry Membro	Universidade de Lisboa Faculdade de Ciências, Portugal
2019/07 - Atual	High-Throughput Screening Facility, Manager Coordenador	Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2019/07 - Atual	FCUL Microscopy Facility, Co-Responsible Coordenador	Universidade de Lisboa Faculdade de Ciências, Portugal

Tutoria

	Tópico	Nome do aluno
2011/01/01 - 2011/01/31	Conformation and Stability of Phenylalanine Hydroxylase	João Leandro
2010/05/01 - 2010/12/31	Molecular basis of the stabilization of the Acidianus ambivalens Rieske Ferredoxin by using rationally designed mutants	Francesco Barbero
2010/03 - 2010/03	Elucidation of the molecular basis of the stabilization of the Acidianus ambivalens Rieske Ferredoxin by using rationally designed mutants	Dennis Petrasch
2009/10/01 - 2009/10/31	Assembly of wild type and mutant Acidianus ambivalens Sulfur Oxygenase Reductase	Andreas Veith
2007/01/01 - 2007/03/31	Conformation and stability of Phenylalanine Hydroxylase	Cátia Nascimento

Distinções

Prémio

2019	Travel grant. NEUBIAS Training School #13. BioImage Analysis for Facility Staff
2018	ITC Conference Grant - EMBL Conference "From Images to Knowledge with ImageJ & Friends" European Cooperation in Science and Technology, Bélgica
2015	Meeting fellowship - 12th ECFS Basic Science Conference European Cystic Fibrosis Society, Dinamarca
2013	Best Poster Award at the meeting “Molecular Biology in Portugal and EMBL"
2011	FEBS Short Term Grant Federation of European Biochemical Societies, Reino Unido
2010	Subsidy for participating in International Congresses - IV Spanish-Portuguese Biophysical Congress) Fundação Calouste Gulbenkian, Portugal
2010	Travel Grant - IV Spanish-Portuguese Biophysical Congress
2010	Travel Grant - IV Spanish-Portuguese Biophysical Congress
2007	Banco Espírito Santo Award – Best Classified Graduates 2005/2006 – Biochemistry Course, Faculty of Sciences, University of Lisbon Banco Espírito Santo e Comercial de Lisboa, Portugal
2007	Student Travel Grant - 13th International Conference on Biological Inorganic Chemistry Society of Biological Inorganic Chemistry, Estados Unidos
2004	Fundação da Faculdade de Ciências da Universidade de Lisboa Award – Best Classified 1st year Graduation Students in 2002/2003 Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal

Outra distinção

2014	Post-Doc Fellowship (SFRH/BD/31126/2006) Fundação para a Ciência e a Tecnologia, Portugal
2012	Post-Doc Fellowship (Reference #7207534) Cystic Fibrosis Foundation, Estados Unidos
2011	Post-Doc Fellowship (Research Project PTDC/QUI/70101/2006) Fundação para a Ciência e a Tecnologia, Portugal
2006	PhD Fellowship (SFRH/BD/31126/2006) Fundação para a Ciência e a Tecnologia, Portugal