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Maria M Pinto holds a Bachelor's degree in Biomedical Sciences with a minor in Pharmaceutical Biotechnology, from the Medical Sciences Department, University of Aveiro, and a Master's degree in Pharmaceutical Biotechnology from the Faculty of Pharmacy, University of Coimbra (Coimbra, Portugal). Additionally, she complimented her education with a summer course in Scientific Research Methodologies and later with a course in Research Methodologies in Life Sciences, Nature Sciences and Engineering, from the Institute of Interdisciplinary Research, University of Coimbra (Coimbra, Portugal). She also attended two online non-credit courses authorized by the University of California (Irvine, USA) offered through Coursera entitled "Basics of Extracellular Vesicles" and "Extracellular Vesicles in Health and Disease" from the International Society for Extracellular Vesicles. During her Master's degree, she developed her thesis entitled "Brain-enriched plasma-derived extracellular vesicles as biomarkers for Machado-Joseph Disease" under the supervision of Doctor Magda M Santana and Professor Luís Pereira de Almeida in Gene and Stem Cell Therapies for the Brain Group Center for Neuroscience and Cell Biology - University of Coimbra (CNC-UC), In January 2021, she was granted a fellowship to continue the work developed in her Master's in the framework of the JPND European Spinocerebellar Ataxia Type 3/Machado-Joseph Disease Initiative (JPCOFUND/0001/2015-ESMI) aiming to identify biomarkers for Machado-Joseph Disease. During her academic/professional journey, she learned several cellular and molecular biology techniques such as plasma isolation from whole blood, iPSCs cell culture; Neural differentiation of iPSCs cultures; Immunocytochemistry; Extracellular vesicles isolation and characterization; DNA/RNA/protein extraction and quantification; cDNA synthesis; qRT-PCR; Western blot and ELISA. As a result of her research work, she is the author and co-author of 5 oral communications and 3 poster presentations in scientific meetings in the field, and, thus far, she is the co-author 3 of journal articles (one published and two submitted). Pursuant to her professional journey, since October 2021 she is also a member of the Ataxia Global Initiative, a worldwide research platform with the goal to facilitate the clinical development of therapies for ataxias. Recently she was granted with a PhD Studentship by the Portuguese Foundation for Science and Technology to develop her PhD project aiming to investigate the role of peripheral ataxin-3 in Machado-Joseph Disease pathogenesis.
Identificação

Identificação pessoal

Nome completo
Maria Manuel Martins Pinto

Nomes de citação

  • Pinto, Maria M
  • Pinto, Maria Manuel
  • Pinto, MM
  • Maria Manuel Pinto
  • Maria M Pinto

Identificadores de autor

Ciência ID
E110-59A6-8747
ORCID iD
0000-0001-6692-1433

Endereços de correio eletrónico

  • mmpinto@cnc.uc.pt (Profissional)

Telefones

Telefone
  • 239820190 (Profissional)

Moradas

  • CNC - Centro de Neurociências e Biologia Celular. Rua Larga, Edifício da Faculdade de Medicina, Pólo I, 2º andar, 3004-504, Coimbra, Coimbra, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Médicas e da Saúde
  • Ciências Médicas e da Saúde - Medicina Básica - Neurociências

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Espanhol; Castelhano Utilizador elementar (A2) Utilizador elementar (A2) Utilizador elementar (A2) Utilizador elementar (A2) Utilizador elementar (A2)
Formação
Grau Classificação
2021/01 - 2021/06
Concluído
Research methodologies in life sciences, nature sciences and engineering (Outros)
Institute of Interdisciplinary Research, University of Coimbra, Portugal
2021 - 2021
Concluído
Extracellular Vesicles in Health and Disease (Outros)
University of California Irvine, Estados Unidos
2018/08 - 2020/12/11
Concluído
Pharmaceutical Biotechnology (Mestrado)
Universidade de Coimbra Faculdade de Farmácia, Portugal
"Brain-enriched plasma-derived extracellular vesicles as biomarkers for Machado-Joseph disease" (TESE/DISSERTAÇÃO)
18
2020/08/01 - 2020/10/30
Concluído
Scientific Research Methodologies (Outros)
Institute of Interdisciplinary Research, University of Coimbra, Portugal
2020/04/16
Concluído
Basics of Extracellular Vesicles (Outros)
University of California Irvine, Estados Unidos
2015 - 2018
Concluído
Biomedical Sciences (Licenciatura)
Especialização em Pharmaceutical Biotechnology
Universidade de Aveiro Departamento de Ciências Médicas, Portugal
15
2012 - 2015
Concluído
Ciências e Tecnologias (Ensino secundário)
Escola Secundária Alves Martins, Portugal
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2021/01/01 - Atual Investigador (Investigação) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019/03 - 2020/12/31 Estagiário de Investigação (Investigação) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2022/11 - Atual PhD Student at CNC - Gene and Stem Cell Therapies for the Brain Group Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal , Portugal
Projetos

Bolsa

Designação Financiadores
2022/11/01 - Atual Machado-Joseph Disease beyond the brain: does peripheral mutant ataxin-3 contribute to disease pathogenesis?
2022.11089.BD
Bolseiro de Doutoramento
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal , Portugal
Fundação para a Ciência e a Tecnologia
Em curso

Outro

Designação Financiadores
2019 - 2020 JPND European Spinocerebellar Ataxia Type 3/Machado-Joseph Disease Initiative
JPCOFUND/0001/2015-ESMI
Bolseiro de Mestrado
Produções

Publicações

Artigo em jornal
  1. Ana Catarina VInhas; Ana Cristina Ferreira; Carina Henriques; Carolina Silva; Catarina Miranda; Daniel Henriques; Daniela Oliveira; et al. "Uma viagem rara", PÚBLICO, 2020, https://www.publico.pt/2020/02/28/ciencia/fotogaleria/viagem-rara-400481.
Artigo em revista
  1. Santana, Magda M.; Gaspar, Laetitia S.; Pinto, Maria M.; Silva, Patrick; Adão, Diana; Pereira, Dina; Ribeiro, Joana Afonso; et al. "A standardized protocol for blood and cerebrospinal fluid collection and processing for biomarker research in ataxia". Neuropathology and Applied Neurobiology (2023): https://doi.org/10.1111/nan.12892.
    Publicado
  2. Maria Soares ; Pinto, Maria Manuel ; Rui Jorge Nobre; Luís Pereira de Almeida; Maria da Graça Rasteiro ; Teresa Almeida-Santos ; João Ramalho-Santos ; Ana Paula Sousa. "Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus Ultracentrifugation". Biomolecules (2023): https://doi.org/10.3390/biom13020278.
    Publicado
  3. Gaspar, Laetitia S; Magda M Santana; Carina Henriques; Pinto, Maria Manuel ; Teresa M Ribeiro Rodrigues; Girao, Henrique; Rui Jorge Nobre; Pereira de Almeida, Luis. "Simple and Fast Size Exclusion Chromatography-based Protocol to Isolate Human Plasma-derived Extracellular Vesicles for Transcriptional Research". Molecular Therapy - Methods & Clinical Development (2020): http://dx.doi.org/10.1016/j.omtm.2020.07.012. 10.1016/j.omtm.2020.07.012.
    Publicado
Poster em conferência
  1. Maria Inês Santos; Ana Daniela Oliveira; Pinto, Maria Manuel ; Magda M Santana; Diana Santos; Ana Rita Álvaro; Pereira de Almeida, Luis (5911-1D34-FB6B); Sónia Duarte. "miRNA Dysregulation in Machado-Joseph Disease: What Can We Learn From Human Induced Pluripotent Stem Cell-Based Models?". Trabalho apresentado em XVII Meeting of the Portuguese Society for Neuroscience, 2021.
  2. Pinto, Maria Manuel. "Biomarkers for Machado-Joseph Disease: MicroRNAs in plasma-derived extracellular vesicles as potential candidates". Trabalho apresentado em Student Network on Extracellular Vesicles International Virtual Conference 2021, 2021.
  3. Magda M Santana; Silva, Patrick; Gaspar, Laetitia S; Pinto, Maria Manuel ; Carina Henriques; Joana Ribeiro; Inês Cunha; et al. "miRNA expression profile of plasma-derived extracellular from Machado-Joseph Disease patients.". Trabalho apresentado em SCA global and ARCA global, 2020.
Pré-impressão
  1. Rufino-Ramos, David; Kevin Leandro; Killian O’Brien; Pedro R.L. Perdigão; Maria Manuel Pinto ; Magda M Santana; Thomas S van Solinge; et al. "Extracellular communication between brain cells through functional transfer of Cre mRNA. Submitted.". 2023. https://doi.org/10.1101/2023.01.29.525937.
Tese / Dissertação
  1. Pinto, Maria M. "Brain-enriched plasma-derived extracellular vesicles as biomarkers for Machado-Joseph Disease". Mestrado, 2020.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2023/02 Extracellular Communication Of Brain Cells Shown Through Functional Transfer Of Cre Mrna LIII Annual meeting of Portuguese Society of Pharmacology
Portuguese Society of Pharmacology (Coimbra, Portugal)
2023/02 Plasma-derived extracellular vesicles from Machado-Joseph Disease/Spinocerebellar Ataxia Type 3 Ataxic patients are enriched in mitochondrial, nuclear and nucleolar small RNA species LIII Annual meeting of Portuguese Society of Pharmacology.
Portuguese Society of Pharmacology. (Coimbra, Portugal)
2022/02/09 Induced Pluripotent Stem cells derived from Machado-Joseph Disease patients as a promising model to investigate microRNA dysregulation. LII Annual meeting of Portuguese Society of Pharmacology
Portuguese Society of Pharmacology (Porto)
2021/02/19 Phenotypic characterization of neuronal cultures derived from Machado Joseph-Disease induced pluripotent stem cells LI Anual Meeting of the Portuguese Society of Pharmacology
Portuguese Society of Pharmacology (Coimbra, Portugal)
2021/02/18 Brain micrornas in plasma-derived extracellular vesicles as potential biomarkers for Machado-Joseph Disease LI Annual Meeting of the Portuguese Society of Pharmacology
Portuguese Society of Pharmacology (Lisboa, Portugal)
2021/02/18 Identification of dysregulated miRNAs in human induced pluripotent stem cell-based models of Machado-Joseph Disease: one step closer to a therapeutic approach? LI Anual Meeting of the Portuguese Society of Pharmacology
Portuguese Society of Pharmacology (Lisboa, Portugal)
2020/10/19 Biomarking a Machado-Joseph disease trial-ready cohort 2nd CIBB Innovative Therapies Retreat
(Coimbra, Portugal)

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2018/04 - Atual Member of the volunteer and organization team of "Competições Nacionais de Ciência" pmate, Competições Nacionais de Ciência
Universidade de Aveiro, Portugal

Membro de comissão

Descrição da atividade
Tipo de participação
Instituição / Organização
2021/10/13 - Atual The Ataxia Global Initiative
Membro
Distinções

Prémio

2022 Best student of Pharmaceutical Biotechnology Masters in 2020
Universidade de Coimbra Faculdade de Farmácia, Portugal