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Ana Maria Sequeira Cardoso concluded her PhD in Biosciences, specialization in Biochemistry, in 2014 from the University of Coimbra (Faculty of Sciences and Technology), her MSc in Biochemistry in 2009 from the University of Coimbra (Faculty of Sciences and Technology) and her BSc in Biochemistry in 2007 from the University of Coimbra (Faculty of Sciences and Technology). She is a researcher in CIBB (former CNC.IBILI), with 35 articles published in specialized journals, and four book chapters, summing 729 citations, which rendered her an h-index of 16 (Scopus). In 2010, Ana Cardoso received a PhD individual grant from the Portuguese Foundation for Science and Technology (FCT) to develop nucleic acid delivery systems, based on lipids, gemini surfactants, cell-penetrating peptides and thermoresponsive polymers, for gene therapy, to be conducted at Center for Neuroscience and Cell Biology, University of Coimbra (CNC). She studied the biophysical properties of nucleic acid delivery systems and established correlations between their biological activity, their structural characteristics and their interaction with biological or model membranes. She also addressed gene therapy directed towards mitochondria. In 2015, after her PhD, which she concluded in 2014 with honors, Ana Cardoso received an individual post-doctoral grant from FCT to study the molecular mechanisms underlying glioblastoma malignancy and to develop RNA-based strategies for glioblastoma therapy. From 2015 to 2019, still at CNC, she studied the role of non-protein coding RNAs in the regulation of energy metabolism in glioblastoma cells and evaluated the impact of gene therapy approaches targeting the expression of long non-coding RNAs and microRNAs involved in the metabolic phenotype of cancer cells, which is intimately connected with stem-like features displayed by tumors, glioblastoma cell invasion ability, and sensitivity to chemotherapeutic drugs. She also developed tridimensional in vitro models of glioblastoma cells (spheroids and organoids), that closely resemble the responses of glioblastoma tumors in their physiological environment, to test RNA-mediated therapeutic approaches. In 2019, Ana Cardoso was hired by CNC under the DL 57/2016 norm (current employment) and engaged in projects featuring the use of iPSCs and human cerebral organoids to study brain diseases with genetic etiology, such as frontotemporal dementia (FTD), and autism spectrum disorders (ASD). She characterized iPSCs reprogrammed from terminally differentiated somatic cells obtained from patients carrying mutations associated with the disease diagnostic, and produced human brain organoids following different protocols. These organoids are being used as platforms for the study of molecular, cellular and network features of neurodevelopment (ASD) and neurodegeneration (FTD). In parallel projects, Ana Cardoso studied miRNAs involved in the immune response in Alzheimer's Disease (AD) patients, including by performing peripheral blood mononuclear cell isolation, identification, determination of immune activity, chemotaxis and phagocytosis, and correlation with differential expression of immune-related microRNAs. She also helped establish a murine asthma model that was used to assess the epidemiological correlation between attention deficit and hyperactivity disorder (ADHD) with immune dysfunction in early childhood.
Identificação

Identificação pessoal

Nome completo
Ana Maria Sequeira Cardoso

Nomes de citação

  • Cardoso, A.M.

Identificadores de autor

Ciência ID
D113-0602-8986
ORCID iD
0000-0002-1629-4782
Researcher Id
D-6563-2014
Scopus Author Id
47961021900

Websites

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências Naturais - Ciências Biológicas - Biofísica
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Diagnóstico e Terapias de Base Genética
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Tecnologias que envolvem a Manipulação de Células,Tecidos,Órgãos ou todo o Organismo

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2)
Francês Utilizador elementar (A1) Utilizador independente (B1) Utilizador elementar (A1) Utilizador elementar (A1)
Formação
Grau Classificação
2014
Concluído
Doutoramento em Biociências, especialização em Bioquímica (Doutoramento)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Nucleic Acid Delivery Systems: From Biophysics to Biological Activity" (TESE/DISSERTAÇÃO)
Aprovada com distinção e louvor por unanimidade
2009
Concluído
Mestrado em Bioquímica (2º ciclo de Bolonha) (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Biophysical characterization of Gemini-based lipoplexes with different levels of biological activity" (TESE/DISSERTAÇÃO)
18
2007
Concluído
Bioquímica (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
14
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2020 - Atual Investigador (Investigação) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019/01/15 - 2020 Investigador (Investigação) CNC.IBILI, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2015/01/02 - 2019/01/14 Post-Doc Researcher CNC.IBILI, Portugal
2010/05/01 - 2014/12/23 Ph.D. student CNC.IBILI, Portugal
2009/09/01 - 2010/04/30 Research Technician CNC.IBILI, Portugal
Projetos

Bolsa

Designação Financiadores
2015/01/02 - 2019/01/14 Targeting non-coding RNAs involved in the regulation of glycolysis/oxidative phosphorylation of glioblastoma cells towards tumor sensitization to chemotherapy
SFRH/BPD/99613/2014
Bolseiro de Pós-Doutoramento
CNC.IBILI, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/05/03 - 2014/04/30 Interaction of Lipoplexes with Cellular Membranes: a Biophysical Approach to Gene Delivery Fundação para a Ciência e a Tecnologia
Concluído

Projeto

Designação Financiadores
2009/09/01 - 2010/04/30 Development of a novel approach for anti-tumoral therapy based on the S4(13)-PV cell penetrating peptide to improve the intracellular delivery of therapeutic proteins and nucleic acids
PTDC/BIO/65627/2006
Bolseiro de Técnico de Investigação
CNC.IBILI, Portugal
Fundação para a Ciência e a Tecnologia
Concluído

Outro

Designação Financiadores
2023/03/01 - 2026/02/28 Th2brain: Effects of Th2-related immunity in the postnatal maturation of brain circuits
2022.02604.PTDC
Investigador
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2022/05/01 - 2025/04/30 Astrocytes dysfunctions in Phelan-McDermid syndrome: from mechanisms towards new therapeutic strategies
NEURON/0002/2021
Co-Investigador Responsável (Co-IR)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2021/01/01 - 2023/12/31 Disease modelling of frontotemporal dementia in human brain organoids
PTDC/MEC-NEU/4814/2020
Investigador
Fundação para a Ciência e a Tecnologia
2019/01/01 - 2020/12/31 Projecto estratégico UID/NEU/04539/2019
UID/NEU/04539/2019
Investigador
Em curso
2017/01/01 - 2020/12/31 BrainHealth2020 - Early Detection, Neuromodulation and Advanced Therapies to Brain Disorders
CENTRO-01-0145-FEDER-000008
Investigador
Em curso
2017/01/01 - 2019/12/31 Tackling CANcer STEM CELls: a challenge and an opportunity to advance in anti-cancer therapy
POCI-01-0145-FEDER-016390
Investigador
Em curso
2016/01/01 - 2018/12/31 A lipidomic and miRNA-based strategy for glioblastoma treatment
A03/2016
Investigador
Concluído
2013/01/01 - 2015/12/31 Combined therapeutic approach based on miRNA modulation towards glioblastoma multiforme: A novel targeted lipid­based nanosystem for systemic delivery
PTDC/DTP-FTO/0265/2012
Investigador
Concluído
2013/01/01 - 2014/12/31 Projecto estratégico CNC/UC: PEst­C/SAU/LA0001/2013 – LA1
CNC/UC: PEst­C/SAU/LA0001/2013 – LA1
Investigador
Concluído
2011/01/01 - 2012/12/31 Projecto estratégico CNC/UC: PEst­C/SAU/LA0001/2011 - LA1
CNC/UC: PEst­C/SAU/LA0001/2011 - LA1
Investigador
CNC.IBILI, Portugal
Concluído
Produções

Publicações

Artigo em revista
  1. Guedes, Joana R.; Ferreira, Pedro A.; Costa, Jéssica; Laranjo, Mariana; Reis, Tiago; Cardoso, Ana Maria; Lebre, Carolina; et al. "IL-4 Shapes Microglia-Dependent Pruning of the Cerebellum During Postnatal Development". SSRN Electronic Journal (2023): http://dx.doi.org/10.2139/ssrn.4281069.
    Em revisão • 10.2139/ssrn.4281069
  2. Catarina M. Morais; Ana M. Cardoso; Ana Rita D. Araújo; Ana Reis; Pedro Domingues; Maria Rosário M. Domingues; Maria C. Pedroso de Lima; Amália S. Jurado. "Stearoyl CoA Desaturase-1 Silencing in Glioblastoma Cells: Phospholipid Remodeling and Cytotoxicity Enhanced upon Autophagy Inhibition". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms232113014.
    10.3390/ijms232113014
  3. Ana Rafaela Oliveira; Solange Martins; Giuseppe Cammarata; Mariana Martins; Ana Maria Cardoso; Maria Rosário Almeida; Maria do Carmo Macário; et al. "Generation and Characterization of Novel iPSC Lines from a Portuguese Family Bearing Heterozygous and Homozygous GRN Mutations". Biomedicines (2022): https://doi.org/10.3390/biomedicines10081905.
    10.3390/biomedicines10081905
  4. Regueira, Patrícia; Silva, Ana Rita; Cardoso, Ana Luísa; Cardoso, Ana Maria; et al.. "Peripheral inflammatory markers during an acute bacterial infection in older patients with and without cognitive dysfunction: a case control study". Brain Behavior and Immunity (2022):
    Submetido
  5. Cardoso, Ana M; Morais, Catarina M; Sousa, Madalena; Rebelo, Olinda; Tão, Hermínio; Barbosa, Marcos; Pedroso de Lima, Maria C; Jurado, Amália S. "MiR-200c-based metabolic modulation in glioblastoma cells as a strategy to overcome tumor chemoresistance". Human Molecular Genetics 30 23 (2021): 2315-2331. http://dx.doi.org/10.1093/hmg/ddab193.
    10.1093/hmg/ddab193
  6. Jéssica Costa; Solange Martins; Pedro A. Ferreira; Ana M.S. Cardoso; Joana R. Guedes; João Peça; Ana L. Cardoso. "The old guard: Age-related changes in microglia and their consequences". Mechanisms of Ageing and Development 197 (2021): 111512-111512. https://doi.org/10.1016/j.mad.2021.111512.
    10.1016/j.mad.2021.111512
  7. Cardoso, A.M.; Morais, C.M.; Rebelo, O.; Tão, H.; Barbosa, M.; Pedroso de Lima, M.C.; Jurado, A.S.. "Downregulation of long non-protein coding RNA MVIH impairs glioblastoma cell proliferation and invasion through a miR-302a-dependent mechanism". Human Molecular Genetics (2021): http://dx.doi.org/10.1093/hmg/ddab009.
    10.1093/hmg/ddab009
  8. Cardoso, A.M.; Morais, C.M.; Pena, F.; Marante, T.; Cunha, P.P.; Jurado, A.S.; Pedroso de Lima, M.C.. "Differentiation of glioblastoma stem cells promoted by miR-128 or miR-302a overexpression enhances senescence-associated cytotoxicity of axitinib". Human Molecular Genetics (2021): http://dx.doi.org/10.1093/hmg/ddab011.
    10.1093/hmg/ddab011
  9. Faria, Rúben; Albuquerque, Tânia; Neves, Ana R.; Bhatt, Himanshu; Biswas, Swati; Cardoso, Ana M.; Pedroso de Lima, Maria C.; Jurado, Amália S.; Costa, Diana. "Physicochemical characterization and targeting performance of triphenylphosphonium nano-polyplexes". Journal of Molecular Liquids 316 (2020): 113873. http://dx.doi.org/10.1016/j.molliq.2020.113873.
    10.1016/j.molliq.2020.113873
  10. Morais, Catarina M.; Cardoso, Ana M.; Aguiar, Luísa; Vale, Nuno; Nóbrega, Clévio; Zuzarte, Mónica; Gomes, Paula; Pedroso de Lima, Maria C.; Jurado, Amália S.. "Lauroylated Histidine-Enriched S413-PV Peptide as an Efficient Gene Silencing Mediator in Cancer Cells". Pharmaceutical Research 37 10 (2020): http://dx.doi.org/10.1007/s11095-020-02904-x.
    10.1007/s11095-020-02904-x
  11. Silva, Francisco; Paulo, António; Pallier, Agnès; Même, Sandra; Tóth, Éva; Gano, Lurdes; Marques, Fernanda; et al. "Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics". Materials 13 3 (2020): 513. http://dx.doi.org/10.3390/ma13030513.
    Publicado • 10.3390/ma13030513
  12. Barros-Viegas, Ana Teresa; Carmona, Vítor; Ferreiro, Elisabete; Guedes, Joana; Cardoso, Ana Maria; Cunha, Pedro; Pereira de Almeida, Luís; et al. "MiRNA-31 improves cognition and abolishes amyloid-ß pathology by targeting APP and BACE1 in an animal model of Alzheimer’s disease". Molecular Therapy - Nucleic Acids (2020): http://dx.doi.org/10.1016/j.omtn.2020.01.010.
    Aceite para publicação • 10.1016/j.omtn.2020.01.010
  13. Catarina M Morais; Pedro P Cunha; Tânia Melo; Ana M Cardoso; Pedro Domingues; M Rosário Domingues; Maria C Pedroso de Lima; Amália S Jurado. "Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma". Human Molecular Genetics (2019): https://doi.org/10.1093/hmg/ddz152.
    10.1093/hmg/ddz152
  14. Ana M S Cardoso; Madalena Sousa; Catarina M Morais; Liliana R Oancea-Castillo; Anne Régnier-Vigouroux; Olinda Rebelo; Hermínio Tão; et al. "MiR-144 overexpression as a promising therapeutic strategy to overcome glioblastoma cell invasiveness and resistance to chemotherapy". Human Molecular Genetics (2019): https://doi.org/10.1093/hmg/ddz099.
    10.1093/hmg/ddz099
  15. Pinto, Sara M. A.; Calvete, Mário J. F.; Ghica, Mariana E.; Soler, Sérgio; Gallardo, Iluminada; Pallier, Agnès; Laranjo, Mariana B.; et al. "A biocompatible redox MRI probe based on a Mn(ii)/Mn(iii) porphyrin". Dalton Transactions 48 10 (2019): 3249-3262. http://dx.doi.org/10.1039/c8dt04775h.
    10.1039/c8dt04775h
  16. Morais, Catarina M.; Cardoso, Ana M.; Cunha, Pedro P.; Aguiar, Luísa; Vale, Nuno; Lage, Emílio; Pinheiro, Marina; et al. "Acylation of the S413-PV cell-penetrating peptide as a means of enhancing its capacity to mediate nucleic acid delivery: Relevance of peptide/lipid interactions". Biochimica et Biophysica Acta (BBA) - Biomembranes 1860 12 (2018): 2619-2634. http://dx.doi.org/10.1016/j.bbamem.2018.10.002.
    10.1016/j.bbamem.2018.10.002
  17. Viegas, A.T.B.; Guedes, J.R.; Oliveira, A.R.; Cardoso, A.M.S.; Cardoso, A.L.C.. "miRNAs: New biomarkers and therapeutic targets in dementia". Current Pharmaceutical Design 23 5 (2017): 669-692. http://www.scopus.com/inward/record.url?eid=2-s2.0-85017530528&partnerID=MN8TOARS.
    10.2174/1381612823666170111094702
  18. Cunha, P.P.; Costa, P.M.; Morais, C.M.; Lopes, I.R.; Cardoso, A.M.; Cardoso, A.L.; Mano, M.; Jurado, A.S.; Pedroso De Lima, M.C.P.. "High-throughput screening uncovers miRNAs enhancing glioblastoma cell susceptibility to tyrosine kinase inhibitors". Human Molecular Genetics 26 22 (2017): 4375-4387. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034256071&partnerID=MN8TOARS.
    10.1093/hmg/ddx323
  19. Cruz, R.Q.; Morais, C.M.; Cardoso, A.M.; Silva, S.G.; Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S.. "Enhancing glioblastoma cell sensitivity to chemotherapeutics: A strategy involving survivin gene silencing mediated by gemini surfactant-based complexes". European Journal of Pharmaceutics and Biopharmaceutics 104 (2016): 7-18. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964330230&partnerID=MN8TOARS.
    10.1016/j.ejpb.2016.04.014
  20. Pinto, S.M.A.; Tomé, V.A.; Calvete, M.J.F.; Pereira, M.M.; Burrows, H.D.; Cardoso, A.M.S.; Pallier, A.; et al. "The quest for biocompatible phthalocyanines for molecular imaging: Photophysics, relaxometry and cytotoxicity studies". Journal of Inorganic Biochemistry 154 (2016): 50-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946887800&partnerID=MN8TOARS.
    10.1016/j.jinorgbio.2015.10.013
  21. Guedes, J.R.; Santana, I.; Cunha, C.; Duro, D.; Almeida, M.R.; Cardoso, A.M.; de Lima, M.C.P.; Cardoso, A.L.. "MicroRNA deregulation and chemotaxis and phagocytosis impairment in Alzheimer's disease". Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring 3 (2016): 7-17. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956620147&partnerID=MN8TOARS.
    10.1016/j.dadm.2015.11.004
  22. Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S.. "New serine-derived gemini surfactants as gene delivery systems". European Journal of Pharmaceutics and Biopharmaceutics 89 (2015): 347-356. http://www.scopus.com/inward/record.url?eid=2-s2.0-84920830778&partnerID=MN8TOARS.
    10.1016/j.ejpb.2014.12.013
  23. Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Cardoso, A.L.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S.. "Gemini Surfactants Mediate Efficient Mitochondrial Gene Delivery and Expression". Molecular Pharmaceutics 12 3 (2015): 716-730. http://www.scopus.com/inward/record.url?eid=2-s2.0-84924023696&partnerID=MN8TOARS.
    10.1021/mp5005349
  24. Cardoso, A.M.; Calejo, M.T.; Morais, C.M.; Cardoso, A.L.; Cruz, R.; Zhu, K.; Pedroso De Lima, M.C.; Jurado, A.S.; Nyström, B.. "Application of thermoresponsive PNIPAAM-b-PAMPTMA diblock copolymers in siRNA delivery". Molecular Pharmaceutics 11 3 (2014): 819-827. http://www.scopus.com/inward/record.url?eid=2-s2.0-84901742907&partnerID=MN8TOARS.
    10.1021/mp400510a
  25. Kjøniksen, A.-L.; Calejo, M.T.; Zhu, K.; Cardoso, A.M.S.; De Lima, M.C.P.; Jurado, A.S.; Nyström, B.; Sande, S.A.. "Sustained release of naltrexone from Poly(N-Isopropylacrylamide) microgels". Journal of Pharmaceutical Sciences 103 1 (2014): 227-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890551451&partnerID=MN8TOARS.
    10.1002/jps.23780
  26. Cardoso, A.M.; Morais, C.M.; Silva, S.G.; Marques, E.F.; De Lima, M.C.P.; Jurado, M.A.S.. "Bis-quaternary gemini surfactants as components of nonviral gene delivery systems: A comprehensive study from physicochemical properties to membrane interactions". International Journal of Pharmaceutics 474 1-2 (2014): 57-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906267891&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2014.08.011
  27. Calejo, M.T.; Cardoso, A.M.S.; Kjøniksen, A.-L.; Zhu, K.; Morais, C.M.; Sande, S.A.; Cardoso, A.L.; et al. "Temperature-responsive cationic block copolymers as nanocarriers for gene delivery". International Journal of Pharmaceutics 448 1 (2013): 105-114. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875930565&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2013.03.028
  28. Trabulo, S.; Cardoso, A.L.; Cardoso, A.M.S.; Morais, C.M.; Jurado, A.S.; Pedroso de Lima, M.C.. "Cell-penetrating peptides as nucleic acid delivery systems: From biophysics to biological applications". Current Pharmaceutical Design 19 16 (2013): 2895-2923. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878655380&partnerID=MN8TOARS.
    10.2174/1381612811319160006
  29. Calejo, M.T.; Cardoso, A.M.S.; Marques, E.F.; Araújo, M.J.; Kjøniksen, A.-L.; Sande, S.A.; de Lima, M.C.P.; Jurado, A.S.; Nyström, B.. "In vitro cytotoxicity of a thermoresponsive gel system combining ethyl(hydroxyethyl) cellulose and lysine-based surfactants". Colloids and Surfaces B: Biointerfaces 102 (2013): 682-686. http://www.scopus.com/inward/record.url?eid=2-s2.0-84867835593&partnerID=MN8TOARS.
    10.1016/j.colsurfb.2012.09.033
  30. Silva, S.G.; Alves, C.; Cardoso, A.M.S.; Jurado, A.S.; Pedroso De Lima, M.C.; Vale, M.L.C.; Marques, E.F.. "Synthesis of gemini surfactants and evaluation of their interfacial and cytotoxic properties: Exploring the multifunctionality of serine as headgroup". European Journal of Organic Chemistry 9 (2013): 1758-1769. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875008251&partnerID=MN8TOARS.
    10.1002/ejoc.201201396
  31. Cardoso, A.M.; Trabulo, S.; Cardoso, A.L.; Maia, S.; Gomes, P.; Jurado, A.S.; Pedroso De Lima, M.C.. "Comparison of the efficiency of complexes based on S413-PV cell-penetrating peptides in plasmid DNA and siRNA delivery". Molecular Pharmaceutics 10 7 (2013): 2653-2666. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879735661&partnerID=MN8TOARS.
    10.1021/mp400078h
  32. Cardoso, A.M.S.; Trabulo, S.; Cardoso, A.L.; Lorents, A.; Morais, C.M.; Gomes, P.; Nunes, C.; et al. "S4(13)-PV cell-penetrating peptide induces physical and morphological changes in membrane-mimetic lipid systems and cell membranes: Implications for cell internalization". Biochimica et Biophysica Acta - Biomembranes 1818 3 (2012): 877-888. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856059612&partnerID=MN8TOARS.
    10.1016/j.bbamem.2011.12.022
  33. Calejo, M.T.; Kjoniksen, A.-L.; Pinazo, A.; Pérez, L.; Cardoso, A.M.S.; Pedroso De Lima, M.C.; Jurado, A.S.; Sande, S.A.; Nyström, B.. "Thermoresponsive hydrogels with low toxicity from mixtures of ethyl(hydroxyethyl) cellulose and arginine-based surfactants". International Journal of Pharmaceutics 436 1-2 (2012): 454-462. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865288628&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2012.07.018
  34. Cardoso, A.M.S.; Faneca, H.; Almeida, J.A.S.; Pais, A.A.C.C.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S.. "Gemini surfactant dimethylene-1,2-bis(tetradecyldimethylammonium bromide)-based gene vectors: A biophysical approach to transfection efficiency". Biochimica et Biophysica Acta - Biomembranes 1808 1 (2011): 341-351. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649777308&partnerID=MN8TOARS.
    10.1016/j.bbamem.2010.09.026
  35. Trabulo, S.; Cardoso, A.M.; Santos-Ferreira, T.; Cardoso, A.L.; Simões, S.; Pedroso De Lima, M.C.. "Survivin silencing as a promising strategy to enhance the sensitivity of cancer cells to chemotherapeutic agents". Molecular Pharmaceutics 8 4 (2011): 1120-1131. http://www.scopus.com/inward/record.url?eid=2-s2.0-79961082146&partnerID=MN8TOARS.
    10.1021/mp100426e
Capítulo de livro
  1. Cardoso, Ana M.; Cardoso, Ana L; Pedroso de Lima, Maria C; Jurado, Amália S. "Non-viral therapeutic approaches for modulation of gene expression: nanotechnological strategies to overcome biological challenges". In Nanoparticles in the Life Sciences and Biomedicine, 409-432. Pan Stanford Publishing, 2018.
  2. Cardoso, Ana. "Gene delivery mediated by gemini surfactants: structure-activity relationships". In Engineering of Nanobiomaterials, 227-256. Elsevier, 2016.
    Publicado • 10.1016/B978-0-323-41532-3.00007-5
  3. Cardoso, Ana. "Recent trends in nanotechnology towards CNS diseases: Lipid-based nanoparticles and exosomes for targeted therapeutic delivery". 2016.
    10.1016/bs.irn.2016.05.002
  4. Trabulo, S.; Cardoso, A.L.; Cardoso, A.M.S.; Düzgünes, N.; Jurado, A.S.; De Lima, M.C.P.. "Cell-penetrating peptide-based systems for nucleic acid delivery: A biological and biophysical approach". In Methods in Enzymology, 277-300. 2012.
    10.1016/B978-0-12-391858-1.00014-9
Poster em conferência
  1. Guedes J.R.; Ferreira P.; Costa J.; Laranjo, M.; Reis, T.; Cardoso, A.M.; et al.. "Allergies and IL-4 shape postnatal cerebellar development via microglia-mediated neuronal pruning to induce ADHD-like behaviors in mice". Trabalho apresentado em 9th FENS Forum 2022, 2022.
  2. Oliveira, A.R.; Cammarata, G.; Cardoso, A.M.; et al. "Autism associated CASPR2 auto-immune antibodies modify the developmental trajectory and network activity in human brain organoids". Trabalho apresentado em 9th FENS Forum 2022, 2022.
  3. Martins, S.; Martins, M.; Neves, M.; Cardoso, A.M.; et al. "Immunocompetent brain organoids and induced-microglia-like cells to study the pathological mechanisms of Frontotemporal Dementia". 2022.
  4. Martins, S.; Martins, M.; Neves, M.; Cardoso, A.M.; et al. "Immunocompetent brain organoids and induced-microglia-like cells to study the pathological mechanisms of Frontotemporal Dementia". 2022.
  5. Neves, M.; Martins, S.; Oliveira, A.R.; Martins, M.; Cammarata, G.; Cardoso, A.M.; et al. "Generation and characterization of induced pluripotent stem cells derived from human dermal fibroblast lines of Frontotemporal Lobar Degeneration patients". 2022.
  6. Oliveira, A.R.; Seabra, C.M.; Guedes, J.R.; Cammarata, G.; Cardoso, A.M.; et al. "Uncovering the neurodevelopmental consequences of anti-CASPR2 antibody exposure in 3D brain organoids". 2021.
  7. Guedes, Joana; Ferreira, Pedro; Laranjo, Mariana; Reis, Tiago; Cardoso, A.M.; et al. "Abnormal microgia-mediated prunning in the cerebellum induces circuitry and behavioral disfunction in mice". Trabalho apresentado em XVII Meeting of the Portuguese Society for Neuroscience, 2021.
  8. Gomes, M; Pereira, M; Laranjo, M; Reis, T; Cardoso, A M; et al. "GPRASP2 (dys)function in metabolism and obesity". Trabalho apresentado em Regional Meeting of the International Society for Neurochemistry Meeting, 2021.
  9. Morais, Catarina; Cardoso, A.M.; at al.. "Silencing of key-enzymes of lipid metabolism combined with multitargeted receptor tyrosine kinase inhibitors as a new therapeutic strategy for glioblastoma". Trabalho apresentado em FEBS 2019 Advanced Course: Oncometabolism: From Conceptual Knowledge to Clinical Applications, 2019.
  10. Cardoso, A.M.; Sousa, Madalena G.; Morais, Catarina. "Combined therapeutic approach to tackle glioblastoma: modulation of miRNAs involved in bioenergetic metabolism for cell sensitization to chemotherapy". Trabalho apresentado em MitoPorto – Advances in Mitochondrial Research, 2017.
  11. Morais, Catarina; Cunha, Pedro; Cardoso, A.M.; et al. "Membrane perturbations induced by acylated S4(13)-PV analogs: relevance to nucleic acid delivery". Trabalho apresentado em 34th European Peptide Symposium, 2016.
  12. Cunha, Pedro; Costa, Raquel; Costa, Pedro; Cardoso, A.M.; et al. "A promising targeted strategy to overcome drug-resistance in glioblastoma". Trabalho apresentado em EMBO workshop: Modern DNA concepts and tools for safe gene transfer and modification, 2015.
  13. Cunha, Pedro; Costa, Pedro; Costa, Raquel; Custódia, Carlos; Branco, Ruben; Morais, Catarina; Cardoso, A.M.; et al. "MicroRNA modulation renders glioblastoma cells sensitive to chemotherapy". Trabalho apresentado em 49th Annual Scientific Meeting of the European Society for Clinical Investigation, 2015.
  14. Cruz, Rita; Morais, Catarina; Cardoso, A.M.; et al. "Gene silencing mediated by gemini-based formulations". Trabalho apresentado em XVIII Congress of the Portuguese Biochemical Society, 2014.
  15. Guedes, Joana; Santana, Isabel; Cunha, Catarina; Almeida, Rosário; Cardoso, A.M.; et al. "Molecular and phenotypic alterations in the mononuclear phagocyte system from Alzheimer’s disease patients". Trabalho apresentado em XVIII Congress of the Portuguese Biochemical Society, 2014.
  16. Cunha, Pedro; Costa, Pedro; Costa, Raquel; Branco, Ruben; Morais, Catarina; Cardoso, A.M.; et al. "MicroRNA-21 and micro RNA-128 modulation sensitizes glioblastoma cells to sunitinib treatement". Trabalho apresentado em XVIII Congress of the Portuguese Biochemical Society, 2014.
  17. Calvete, Mario; Pinto, Sara; Cardoso, A.M.; et al. "A biocompatible manganese (III) phthalocyanine for development of MRI contrast agents". Trabalho apresentado em COST Action TD1004, 2014.
  18. Morais, Catarina; Cardoso, A.M.; et al. "Nuclear and mitochondrial gene transfection mediated by cationic pentacyclic triterpenoid derivatives". Trabalho apresentado em GEM/GERLI Lipidomics Meeting: From Membranes to Pathologies, 2013.
  19. Cardoso, A.M.; et al. "The structural architecture of Bis-quat gemini surfactants is crucial for their DNA delivery ability". Trabalho apresentado em 9th EBSA – European Biophysics Congress, 2013.
  20. Silva, Sandra; Alves, Claudia; Cardoso, A.M.; et al. "Rational design of cleavable cationic gemini surfactants: exploring the multifunctionality of serine as headgroup". Trabalho apresentado em RICI5 – 5th Iberian Meeting on Colloids and Interfaces, 2013.
  21. Cardoso, A.M.; et al. "Gemini Surfactant-Based Gene Delivery Systems: Transfection Efficiency And Cytotoxicity Depend On Hydrocarbon Chain Length And Helper Lipids". Trabalho apresentado em III Congress of the Portuguese Society of Pharmaceutical Sciences/IX Spanish-Portuguese Conference on Controlled Drug Delivery - New Trends in Pharmacological Sciences, 2011.
  22. Cardoso, A.M.; Morais, Catarina; et al. "Transfection efficiency of Gemini surfactant-based gene delivery systems as affected by helper lipids". Trabalho apresentado em RICI4 - 4th Iberian Meeting on Colloids and Interfaces, 2011.
  23. Cardoso, A.M.; et al. "A biophysical study on the interaction of S4(13)-PV cell penetrating peptide with model lipid membranes". Trabalho apresentado em Meeting of Peptide Vectors and Delivery of Therapeutics, 2011.
  24. Cardoso, A.M.; et al. "A biophysical study on the interaction of S413-PV cell penetrating peptide with model lipid membranes". Trabalho apresentado em XVII Congresso Nacional de Bioquímica, 2010.
  25. Trabulo, Sara; Cardoso, A.M.; et al. "Silencing of survivin enhances the sensitivity of cancer cells to chemotherapeutic agents". Trabalho apresentado em 21st Meeting of the European Association for Cancer Research, 2010.
  26. Cardoso, A.M.; et al. "Biophysical properties and transfection ability of ethanediyl-alfa,omega-bis(-N-tetradecyl,N,N’,dimethylammonium bromide)-based vectors". Trabalho apresentado em International Courses on Toxicology, 2010.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2021 Multiparametric MRI characterization of the G144 GBM model 38th Annual Scientific Meeting of the European Society for Magnetic resonance in Medicine and Biology (ESMRMB)
(Barcelona, Espanha)
2019 Long Non-Protein Coding RNA MVIH Silencing as a Therapeutic Approach for Glioblastoma 3rd International Symposium on Frontiers in Molecular Science - RNA Regulatory Networks
(Lisboa, Portugal)
2019 Silencing of key-enzymes of lipid metabolism combined with multitargeted receptor tyrosine kinase inhibitors as a new therapeutic strategy for glioblastoma II FEBS Advanced Lecture Course on Oncometabolism-From Conceptual Knowledge to Clinical Applications
(Luso, Portugal)
2019 Upgrading S413-PV peptide to mediate gene silencing in cancer cells through peptide lauroylation and histidine-enrichment III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer
(Covilhã, Portugal)
2018 Gold Nanoparticles as Multimodal Imaging Agents XIX Brazilian Meeting on Inorganic Chemistry, VI Latin American Meeting on Biological Inorganic Chemistry, VIII Brazilian Meeting on Rare Earths
(Fortaleza, Brasil)
2017 Membrane lipids: protein partners for good and for evil. A case study in glioblastoma 6th Workshop in Lipidomics
(Aveiro, Portugal)
2016 Interaction of acylated S413-PV analogs with lipid membranes 15th Iberian Peptide Meeting
(Porto, Portugal)
2014 The interaction of gene delivery systems with membranes as a modulator of transfection efficiency 3rd Workshop in Lipidomics
(Aveiro, Portugal)
2014 Improving the ability of S4(13)PV cell-penetrating peptide to mediate siRNA delivery through peptide acylation SFNano In vitro/in vivo correlation workshop – Relevant tools and models for the translation of advanced drug delivery systems
(Porto, Portugal)
2014 Gemini surfactants as gene delivery systems: disclosing the properties leading to transfection activity 20th International Symposium on Surfactants in Solution
(Coimbra, Portugal)
2013 Gene Transfer Mediated by Bis-Quaternary Gemini Surfactants Depends on Complex Architecture RICI5 – 5th Iberian Meeting on Colloids and Interfaces
(San Sebastian, Espanha)
2013 Efficient siRNA delivery mediated by S4(13)-PV cell penetrating peptide-based systems: a comparative study 13th Naples Workshop on Bioactive Peptides – Conformation and Activity in Peptides: Relationships and Interactions
(Nápoles, Itália)
2012 Towards a biocompatible thermo-responsive hydrogel for pharmaceutical applications – The combined system of ethyl(hydroxyethyl) cellulose and lysine-based surfactants CRS Nordic Chapter - Drug Delivery and Targeting
(Reykjavik, Islândia)
2011 Membrane lipid interactions with cell-penetrating peptides: lessons on the role of lipids in membrane functioning RICI4 - 4th Iberian Meeting on Colloids and Interfaces
(Porto, Portugal)
2011 Membrane lipid interactions with cell-penetrating peptides: lessons on the role of lipids in membrane functioning RICI4 - 4th Iberian Meeting on Colloids and Interfaces
(Porto, Portugal)
2011 Transfection efficiency of Gemini surfactant-based gene delivery systems as affected by nature of formulation and method of preparation XXXVI Jornadas Portuguesas de Genética
(Coimbra, Portugal)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2022/09 - 2023/09 The Hidden Player: Non-canonical role of Shank3 in neurodevelopment
Orientador
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2023/01 - 2023/08 The Role of Shank3 Mutation in Primary Cilia Length and Function during Neural Development
Coorientador
Vrije Universiteit Amsterdam, Países Baixos
2015/09/01 - 2016/09/10 MiRNA-based metabolic modulation in glioblastoma cells: a strategy to surpass tumor chemoresistance
Orientador
CNC.IBILI, Portugal

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2023/05 - Atual Science Communication for pre-school kids
Mesa-redonda
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/10 - 2017/11 Nature Masterclasses Online Course: Focus on Peer Review
Outro
Focus on Peer Review
Springer Nature, Luxemburgo
2017/01 - 2017/02 Coursera Online Course: Design and Interpretation of Clinical Trials
Outro
Design and Interpretation of Clinical Trials
Johns Hopkins University Center for Global Health, Estados Unidos
2016/10 - 2016/11 Coursera Online Course: Basics of Extracellular Vesicles
Outro
Basics of Extracellular Vesicles
University of California Irvine Cancer Research Institute, Estados Unidos
2016/03 - 2016/05 Coursera Online Course: Epigenetic Control of Gene Expression
Outro
Epigenetic Control of Gene Expression
The University of Melbourne, Austrália
2015/06 - 2015/06 Course: MiRNAs and non-coding RNAs in cell biology and human disease
Outro
MiRNAs and non-coding RNAs in cell biology and human disease
Instituto de Medicina Molecular, Portugal
2013/09 - 2013/09 Course in Laboratorial Animal Science, category C of FELASA
Outro
Course in Laboratorial Animal Science
Universidade de Coimbra, Portugal
2012/06 - 2012/06 Course of Science Communication for Researchers
Outro
Course of Science Communication for Researchers
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Júri de grau académico

Tema
Tipo de participação
Nome do candidato (Tipo de grau)
Instituição / Organização
2023/10/20 Immunotolerance Strategies to Improve Cell-Based Therapy Outcomes in MJD
Arguente principal
Luís Carlos Oliveira (Mestrado)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2022/10/28 Characterization of MJD patient's IPSC-derived NESC silenced for mutant ATAXIN-3
Arguente principal
Mariana Machado (Mestrado)
Universidade de Coimbra Faculdade de Medicina, Portugal
2016/07/07 Avaliação do efeito da mutação c.900-901dupGT do gene GRN na resposta imune e autofagia: um estudo em portadores heterozigóticos e homozigóticos
Arguente principal
Catarina Isabel Carvalhas de Almeida (Outro)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2021 - 2022 Electrophysiology of plasma membranes: Patch clamp CELL CULTURE AND TISSUE TRAINING COURSE (Outros) Universidade de Coimbra Faculdade de Medicina, Portugal
2018 - 2019 LabTalks Exploring the role of circulating miRNAs in Delirium and Dementia (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2018 - 2019 LabTalks Targeted gene/drug therapies for brain tumors: from the immune microenvironment to the stem cell niche (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2016 - 2019 Lnc-RNAs for cancer therapy Advanced Therapies (Curso de doutoramento (conclusão de unidades curriculares)) Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2013 - 2014 Lipidómica Funcional MSc in Biochemistry (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2011 - 2014 Toxicologia Bioquímica e Biofísica Bioquímica (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2011 - 2012 Biomembranas MSc in Biochemistry (Mestrado) Universidade de Coimbra Departamento de Ciências da Vida, Portugal

Tutoria

Tópico Nome do aluno
2019 - 2021 Extracellular vesicles and their role in the tumor microenvironment of glioblastoma multiforme Guilherme Faria
2019 - 2020 Isolation and characterization of extracellular vesicles produced from glioblastoma cells and assessment of the impact of temozolomide in glioblastoma spheroid invasion Inês Oliveira
2019 - 2020 Modelos celulares in vitro para o estudo de terapias para glioblastoma: Vantagens e desvantagens Susana Gomes
2018 - 2019 Isolamento e caracterização de exossomas de células de glioblastoma Francisco Tiago
2018 - 2019 Validação de um protocolo de fracionamento celular para quantificação de proteína em diferentes compartimentos celulares Inês Oliveira
2014 - 2015 Modulação dos níveis de miRNAs envolvidos em alterações metabólicas em linhas celulares de glioblastoma (U87 e DBTRG) como abordagem terapêutica Catarina Luz
2014 - 2015 Estratégia terapêutica para glioblastoma baseada no silenciamento de um gene do metabolismo lipídico combinado com a utilização de um fármaco quimioterapêutico Márcia Teixeira
2012 - 2013 Surfactantes Gemini Catiónicos no Transporte de Material Genético: Abordagem Biológica e Biofísica Ana Rita Cruz
Distinções

Outra distinção

2015 Bolsa Individual de Pos-Doutoramento
Fundação para a Ciência e a Tecnologia, Portugal
2010 Bolsa Individual de Doutoramento
Fundação para a Ciência e a Tecnologia, Portugal