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Mariana Ladeiro Afonso holds a Bachelor’s degree in Biotechnology, a Master’s degree in Biochemistry (with an Erasmus+ mobility period in Poland), and is currently a PhD candidate of the Doctoral Programme in Health Sciences (Biomedical Sciences specialisation) at the Faculty of Medicine, University of Coimbra, supported by the Fundação para a Ciência e a Tecnologia (FCT) doctoral fellowship. Her research explores host–pathogen interactions, focusing on the role of 3’-nucleotidase/nuclease and extracellular nucleotide metabolism in the ability of Candida spp. to evade Neutrophil Extracellular Traps (NETs) and persist within the host, integrating biochemical, cellular, genetic, and in vivo approaches to investigate fungal pathogenic fitness. Mariana has authored two peer-reviewed publications, with two additional manuscripts currently in preparation/ under submission, and has presented her work at multiple national and international scientific meetings. She completed certified training in Laboratory Animal Science (FELASA C) and has undertaken short-term research stays at the University of Würzburg (Germany) and the Universidade Federal do Rio de Janeiro (Brazil), strengthening her international scientific collaboration. She has mentored laboratory training of undergraduate and master’s students and has been recognised with a poster award at a scientific conference. She has also been actively involved in science communication initiatives.
Identificação

Identificação pessoal

Nome completo
Mariana Ladeiro Afonso

Nomes de citação

  • Afonso, Mariana

Identificadores de autor

Ciência ID
D01A-B93A-2D56
ORCID iD
0000-0001-6437-697X

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Inglês Utilizador proficiente (C2) Utilizador independente (B2) Utilizador proficiente (C2) Utilizador proficiente (C1)
Formação
Grau Classificação
2025
Em curso
Doctoral Programme in Health Sciences (Doutoramento)
Especialização em Ciências Biomédicas
Faculdade de Medicina da Universidade de Coimbra, Portugal
"Candida spp. escape. to Neutrophil Extracellular Traps: Role of 3'-nucelotidase/nuclease" (TESE/DISSERTAÇÃO)
2022/11 - 2022/11
Concluído
VI Modular Training in Laboratory Animal Science (Felasa C) (Diploma de especialização)
Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal
2021/01 - 2021/06
Concluído
Training Course – Research Methodologies in Life Sciences, Nature Sciences and Engineering (Diploma de especialização)
Universidade de Coimbra, Portugal
2018/10/05 - 2020/11/13
Concluído
Master's degree in Biochemistry (Mestrado)
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
"Involvement of the purinergic system in the host-microbe interaction" (TESE/DISSERTAÇÃO)
17
2014/10/25 - 2017/11/28
Concluído
Bachelor's degree in Biotechnology (Licenciatura)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
"Quality control mechanisms are up-regulated in epicardial adipose tissue from heart failure patients." (TESE/DISSERTAÇÃO)
14
Percurso profissional

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2025/12 - 2026/02 Research Fellow - Path&D - Alternaria alternata proteome-based novel diagnostics and immunotherapeutocs for allergies (COMPETE2030-FEDER-00676700 | Project 15672) Universidade de Coimbra, Portugal
2022/01 - 2026/02 Research Collaborator - VARCAL – Roles of Candida albicans Variants in Its Interaction with the Human Host (Project 2022.01376.PTDC) Universidade de Coimbra, Portugal
2025/10/23 - 2025/12/19 Short-term research stay at UFRJ-Universidade Federal do Rio de Janeiro, Brazil, under supervision of the Prof. Dr. Robson Coutinho-Silva from Instituto de Biofísica Carlos Chagas Filho (IBCCF) Faculdade de Medicina da Universidade de Coimbra, Portugal
Universidade Federal do Estado do Rio de Janeiro, Brasil
2022/01 - 2025/12 Research Colaborator - PREDICT2TREAT – Candida and Host Genetic Variations as Predictive Risk Factors for Dysbiosis and Inflammation in the Elderly (COMPETE2030-FEDER-00721800 | Project 16069) Universidade de Coimbra, Portugal
2024/08/01 - 2025/08/31 Short-term research stay at the University of Würzburg (Germany), under the supervision of the Prof. Dr. Joachim Morschhäuser from IMIB - Institute of Molecular Infection Biology Faculdade de Medicina da Universidade de Coimbra, Portugal
Julius-Maximilians-Universität Würzburg, Alemanha
2021/03 - 2021/10 Research Fellow - Participation in the validation and performance assessment of air purification equipment developed within the R&D project Pure Air@Automotive (POCI-01-02B7-FEDER-070195) Universidade de Coimbra, Portugal
2020/04/01 - 2020/12/31 Voluntary collaboration in the diagnosis of COVID-19 / SARS-CoV-2 Instituto de Microbiologia Médica - Faculdade de Medicina da Universidade de Coimbra, Portugal
2017/05 - 2017/10 Professional Internship - "Quality control mechanisms are up-regulated in epicardial adipose tissue from heart failure patients.". Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Projetos

Bolsa

Designação Financiadores
2025/12 - 2026/02 Path&D - Alternaria alternata proteome-based novel diagnostics and immunotherapeutocs for allergies
COMPETE2030-FEDER-00676700– n.º15672
Bolseiro de Investigação
European Regional Development Fund
Em curso
2021/12 - 2025/12 Candida spp. escape to Neutrophil Extracellular Traps: Role of 3’-nucleotidase/nuclease
2021.07033.BD
Bolseiro de Doutoramento
Fundação para a Ciência e a Tecnologia, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2021/03 - 2021/10 Participation in the validation and performance assessment of air purification equipment developed within the R&D project Pure Air@Automotive (POCI-01-02B7-FEDER-070195)
POCI-01-02B7-FEDER-070195
Bolseiro de Investigação
European Regional Development Fund
Em curso

Projeto

Designação Financiadores
2020/01/01 - 2024/12/31 Centro de Inovação em Biomedicina e Biotecnologia
UIDP/04539/2020
Universidade de Coimbra, Portugal

Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em revista
  1. Afonso, Mariana; Rodrigues, Lisa ; Lindo, Jorge; Cunha, Rodrigo A.; Savio, Luiz Eduardo Baggio; Coutinho-Silva, Robson; Gonçalves, Teresa. "Extracellular purinergic metabolism and signaling in the interaction between microbial agents and animal/host". TRENDS in Immunology (2026):
    Submetido
  2. Correia, Gil; Rodrigues, Luís; Afonso, Mariana; Mota, Marta; Oliveira, Joana; Soares, Rui; Tomás, Ana Luísa; et al. "SARS-CoV-2 air and surface contamination in residential settings". Scientific Reports 12 1 (2022): http://dx.doi.org/10.1038/s41598-022-22679-y.
    Publicado • 10.1038/s41598-022-22679-y
  3. Afonso, Mariana; Mestre, Ana Rita; Silva, Guilherme; Almeida, Ana Catarina; Cunha, Rodrigo A.; Meyer-Fernandes, José Roberto; Gonçalves, Teresa; Rodrigues, Lisa. "Candida extracellular nucleotide metabolism promotes Neutrophils Extracellular Traps escape". Frontiers in Cellular and Infection Microbiology (2021): DOI: 10.3389/fcimb.2021.678568.
    Publicado
Poster em conferência
  1. Afonso, Mariana; Domingues, Rita; Bandeira, Bernardo; Cunha, Rodrigo A.; Gonçalves, Teresa; Rodrigues, Lisa. "Role of 3' - nucleotidase/nuclease in Candida spp. - neutrophil interaction.". Trabalho apresentado em Innovative Therapies 5th CiBB Retreat, 2025.
  2. Domingues, Rita; Afonso, Mariana; Bandeira, Bernardo; Rodrigues, Lisa ; Gonçalves, Teresa. "Priming the immune system: How Candida albicans Extracellular Vesicles can influence macrophage responses to infection.". Trabalho apresentado em 3rd PNEV Meeting 2025, Faro, Portugal, 2025, 2025.
  3. Afonso, Mariana; Bandeira, Bernardo; Cunha, Rodrigo A.; Rodrigues, Lisa ; Gonçalves, Teresa. "Candida albicans isolated from different infection sites display different ectonucleotidase activity". Trabalho apresentado em 2nd European Purine Meeting 2024, 2024.
  4. Afonso, Mariana; Bandeira, Bernardo; Cunha, Rodrigo A.; Meyer-Fernandes, José Roberto; Gonçalves, Teresa; Rodrigues, Lisa. "Candida albicans 5’-3’-ectonucleotidase activity: a comparative study of isolates from different human infection niches.". Trabalho apresentado em Microbiotec23- Congress of Microbiology and Biotechnology 2023, 2023.
  5. Rodrigues, Lisa ; Afonso, Mariana; Almeida, Ana Catarina; Cunha, Rodrigo A.; Gonçalves, Teresa. "Host CD73 modulates Candida albicans driven neutrophils extracellular traps formation". Trabalho apresentado em XII Meeting of the Brazilian Purine Club - Curitiba, Brazil, 2023, 2023.
  6. Afonso, Mariana; Rodrigues, Lisa ; Almeida, Ana Catarina; Cunha, Rodrigo A.; Gonçalves, Teresa. "Host ecto-5'-nucleotidase (CD73) suppression impairs neutrophils NET formation response upon Candida albicans infection". Trabalho apresentado em 11th Congress on Trends in Medical Mycology (TIMM-11) - Athens, Greece, 2023, 2023.
  7. Domingues, Rita; Afonso, Mariana; Calheiros D.; Nogueira, Célia; Strader, Michael Brad; Slater, Jay E.; Todo-Bom, Ana; et al. "Characterization of Alternaria alternata extracellular vesicles. Proteome and interaction with host cells.". Trabalho apresentado em 2nd Meeting of the Portuguese Network Vesicles, 2023.
  8. Afonso, Mariana; Mestre, Ana Rita; Silva, Guilherme; Almeida, Ana Catarina; Cunha, Rodrigo A.; Meyer-Fernandes, José Roberto; Gonçalves, Teresa; Rodrigues, Lisa. "Candida - Neutrophil interaction: The Role of Extracellular Nucleotide Metabolism.". Trabalho apresentado em FEBS Advanced Lecture Course HFP2022: “Molecular Mechanisms of Host-Pathogen Interactions and Virulence in Human Fungal Pathogens”, 2022.
  9. Afonso, Mariana; Mestre, Ana Rita; Silva, Guilherme; Cunha, Rodrigo A.; Meyer-Fernandes, José Roberto; Gonçalves, Teresa; Rodrigues, Lisa. "Extracellular nucleotide metabolism in the context of Candida-neutrophils interaction.". Trabalho apresentado em Candida and Candidiasis 2021 online congress, 2021.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2025/09/05 Role of 3'-nucleotidase/nuclease in Candida spp. - neutrophil interaction (presented by Mariana Afonso). Innovative Therapies 5th CiBB Retreat
(Coimbra, Portugal)
2024/12/20 Contribution of purinergic signalling to fungal survival and persistence inside host cells (presented by Lisa Rodrigues). CiBB Meeting 2024, Centre for Innovative Biomedicine and Biotechnology, Portugal.
(Lousã, Coimbra, Portugal)
2024/09/21 Candida spp. extracellular metabolism of purines contribution to immune evasion (presented by Lisa Rodrigues). XXII Jornadas de Biologia de Leveduras Professor Nicolau van Uden
(Curia, Portugal)
2024/05 Participation in the initiative “Meet My Science”, delivering a presentation on ongoing research work associated with the PhD project entitled “Candida spp. Escape to Neutrophil Extracellular Traps: Role of 3’-nucleotidase/nuclease.”

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2020 - 2025 Collaboration in the supervision and laboratory training of students from the Master’s Degree in Molecular and Cellular Biology and the Master’s Degree in Biochemistry at the Faculty of Sciences and Technology of the University of Coimbra, as well as from the Master’s Degree in Biomedical Investigation (MIB) at the Faculty of Medicine of the University of Coimbra.
Coorientador

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2024/09/20 - 2024/09/21 Member of the Local Organizing Committee of XXII Jornadas de Biologia de Leveduras Professor Nicolau van Uden, a national congress on Yeasts Biology, held at Curia, Portugal. (2024/09/20 - 2024/09/21)

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2024/04 - 2024/04 Participation in the VIII meet. Lab Coimbra delivered a workshop entitled “Assessment of Antimicrobial Resistance”, organised by the Biology Students’ Association of the University of Coimbra.
2023/09 - 2023/09 Participation in the 7th edition of the International Microorganism Day, promoting microbiology research to the general public.
2021/09/17 - 2021/09/17 Science Communication Activities: Participation in the MicroScience Photo Gallery- Microorganismos de estimação: associated to the International Microorganism Day 2021, Exploratório - Ciência Viva, Coimbra, Portugal.
2020/10/17 - 2020/11/05 Science Communication Activities: Participation in the MicroScience Photo Gallery- Microorganismos de estimação: associated to the International Microorganism Day 2020, Exploratório - Ciência Viva, Coimbra, Portugal.
Exposição
2016/03/16 - 2016/03/17 Participation in Biotechnology Days - Católica Faculty of Biotechnology, Porto, Portugal.
Congresso
Biotechnology Days
Distinções

Prémio

2025 Best Poster Award, for the poster entitled ‘Role of 3’ - nucleotidase/nuclease in Candida spp. –neutrophil interaction’ , in Topic 4 - Health Microbiology, awarded at the 2025 Congress of Microbiology and Biotechnology