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Diego Fernando Gauto. Works in the area(s) of Natural sciences with emphasis on Biological Sciences with emphasis on Biology. I originally started my undergraduate studies in the medical school at the University of Buenos Aires, Argentina in 1999. However, during those years of a dedicate training as a medical doctor I felt mind-boggled by the fact that the chemistry are the fundamental basis of everything that are occurring in our bodies in all the stages of the normal physiology and also the pathology. Therefore, I decided to abruptly turn the pages and begin a fully dedicated career in chemical sciences. For this reason, in 2003 I started to study chemistry in the school of sciences at Buenos Aires University, Argentina. The discovery of the chemistry and the scientific world was one of the most amazing and impressive things that it happens to me and therefore, I decided to continue fully dedicated on a scientific path to finalize my chemistry master degree at the beginning of 2009 and dedicate my scientific career to understand the structural and dynamics details underlying the proteins combining computational simulation (PhD) and NMR experiments (postdocs) on challenging systems. In 2020 I obtained a CNRS position in France (a permanent position that give scientific independence). Therefore, nowadays I am a scientist with a dual background that range from computational biology (PhD) to NMR and structural biology (postdocs). My publication record also reflect my background (35% and 18% of my peer-reviewed publications are in NMR and integrative structural biology respectively, while the remaining 47% are in computational biology) and beyond the number of citations my scientific work was attracted, I was invited to make a talk in 2018 at the CCPN conference, UK and recently invited as guest editor by the journal Biomolecular Biosciencies with the goal to open an issue on integrative structural biology perspectives for the next year. To conclude, my dual background in computational biology and experimental structural biology represent an important asset at the time to foster integrative projects that combine simulation and experiments in order to reach a high synergy and efficiency between all the members of the team.
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Identificação

Identificação pessoal

Nome completo
Diego Fernando Gauto

Nomes de citação

  • Gauto, Diego Fernando

Identificadores de autor

Ciência ID
B710-A702-0CE8
ORCID iD
0000-0003-3757-2830
Google Scholar ID
https://scholar.google.com/citations?user=xb25j5cAAAAJ&hl=en

Endereços de correio eletrónico

  • diego.gauto@cnrs.fr (Profissional)

Moradas

  • 1, avenue de la Terrasse, 91198, Gif-sur-Yvette, Ile de france, França (Profissional)

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Biologia

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Espanhol; Castelhano (Idioma materno)
Inglês Utilizador proficiente (C1) Utilizador proficiente (C2) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C2)
Francês Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B1) Utilizador independente (B2) Utilizador elementar (A2)
Alemão Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1)
Formação
Grau Classificação
2009/04/01 - 2013/12/12
Concluído
 computational biology (Doctor of Philosophy)
Universidad de Buenos Aires Facultad de Ciencias Exactas y Naturales, Argentina
"Fisicoquímica del agua en la superficie proteica y su protagonismo en la unión de carbohidratos Protein’s surface water molecules and their rolein the carbohydrate recognition process" (TESE/DISSERTAÇÃO)
2003/03/01 - 2009/03/01
Concluído
 Chemistry (Master)
Universidad de Buenos Aires, Argentina
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2020/10/01 - Atual Assistente de Investigação (carreira) (Investigação) Centre National de la Recherche Scientifique, França
Institut de Chimie des Substances Naturelles, França
Produções

Publicações

Artigo em revista
  1. Diego Gauto; Pavel Macek; Duccio Malinverni; Hugo Fraga; Matteo Paloni; Iva Sucec; Audrey Hessel; et al. "Functional control of a 0.5 MDa TET aminopeptidase by a flexible loop revealed by MAS NMR". Nature Communications (2022): https://hal.archives-ouvertes.fr/hal-03863696.
    10.1038/s41467-022-29423-0
  2. Diego F. Gauto; Pavel Macek; Duccio Malinverni; Hugo Fraga; Matteo Paloni; Iva Sucec; Audrey Hessel; et al. Autor correspondente: Paul Schanda. "Functional control of a 0.5 MDa TET aminopeptidase by a flexible loop revealed by MAS NMR". Nature Communication (2022):
    No prelo
  3. Diego Gauto; Ons Dakhlaoui; Ildefonso Marin-Montesinos; Sabine Hediger; Gaël De Paëpe. "Targeted DNP for biomolecular solid-state NMR". Chemical Science (2021): https://doi.org/10.1039/D0SC06959K.
    10.1039/D0SC06959K
  4. Gauto, Diego Fernando. "Mechanism of the allosteric activation of the ClpP protease machinery by substrates and active-site inhibitors.". Science advances (2019): https://europepmc.org/articles/PMC6726451.
    10.1126/sciadv.aaw3818
  5. Gauto, Diego Fernando. "Aromatic Ring Dynamics, Thermal Activation, and Transient Conformations of a 468 kDa Enzyme by Specific 1H-13C Labeling and Fast Magic-Angle Spinning NMR.". Journal of the American Chemical Society (2019): https://doi.org/10.1021/jacs.9b04219.
    10.1021/jacs.9b04219
  6. Gauto, Diego Fernando. "Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex.". Nature communications (2019): https://europepmc.org/articles/PMC6584647.
    10.1038/s41467-019-10490-9
  7. Gauto, Diego Fernando. "Mechanistic Insights into Microsecond Time-Scale Motion of Solid Proteins Using Complementary 15N and 1H Relaxation Dispersion Techniques.". Journal of the American Chemical Society (2019): https://europepmc.org/articles/PMC6982537.
    10.1021/jacs.8b09258
  8. Gauto, Diego Fernando. "Microsecond Protein Dynamics from Combined Bloch-McConnell and Near-Rotary-Resonance R1p Relaxation-Dispersion MAS NMR.". Chemphyschem : a European journal of chemical physics and physical chemistry (2018): https://europepmc.org/articles/PMC6354937.
    10.1002/cphc.201800935
  9. Suárez, I.P.; Gauto, D.F.; Hails, G.; Mascali, F.C.; Crespo, R.; Zhao, L.; Wang, J.; Rasia, R.M.. "Conformational sampling of the intrinsically disordered dsRBD-1 domain from: Arabidopsis thaliana DCL1". Physical Chemistry Chemical Physics 20 16 (2018): 11237-11246. http://www.scopus.com/inward/record.url?eid=2-s2.0-85046271912&partnerID=MN8TOARS.
    10.1039/c7cp07908g
  10. Zhao, L.; Suarez, I.P.; Gauto, D.F.; Rasia, R.M.; Wang, J.. "The key role of electrostatic interactions in the induced folding in RNA recognition by DCL1-A". Physical Chemistry Chemical Physics 20 14 (2018): 9376-9388. http://www.scopus.com/inward/record.url?eid=2-s2.0-85045115441&partnerID=MN8TOARS.
    10.1039/c7cp07889g
  11. Krushelnitsky, A.; Gauto, D.; Rodriguez Camargo, D.C.; Schanda, P.; Saalwächter, K.. "Microsecond motions probed by near-rotary-resonance R 1¿15N MAS NMR experiments: the model case of protein overall-rocking in crystals". Journal of Biomolecular NMR 71 1 (2018): 53-67. http://www.scopus.com/inward/record.url?eid=2-s2.0-85047659731&partnerID=MN8TOARS.
    10.1007/s10858-018-0191-4
  12. Diego F. Gauto; Audrey Hessel; Petra Rovó; Vilius Kurauskas; Rasmus Linser; Paul Schanda. "Protein conformational dynamics studied by 15 N and 1 H R 1¿ relaxation dispersion: Application to wild-type and G53A ubiquitin crystals". Solid State Nuclear Magnetic Resonance 87 (2017): 86-95. https://doi.org/10.1016%2Fj.ssnmr.2017.04.002.
    10.1016/j.ssnmr.2017.04.002
  13. Fraga, H.; Arnaud, C.-A.; Gauto, D.F.; Audin, M.; Kurauskas, V.; Macek, P.; Krichel, C.; et al. "Solid-State NMR H–N–(C)–H and H–N–C–C 3D/4D Correlation Experiments for Resonance Assignment of Large Proteins". ChemPhysChem 18 19 (2017): 2697-2703. http://www.scopus.com/inward/record.url?eid=2-s2.0-85028894853&partnerID=MN8TOARS.
    10.1002/cphc.201700572
  14. Salvador I. Drusin; Irina P. Suarez; Diego F. Gauto; Rodolfo M. Rasia; Diego M. Moreno. "dsRNA-protein interactions studied by molecular dynamics techniques. Unravelling dsRNA recognition by DCL1". Archives of Biochemistry and Biophysics 596 (2016): 118-125. https://doi.org/10.1016%2Fj.abb.2016.03.013.
    10.1016/j.abb.2016.03.013
  15. Vilius Kurauskas; Elodie Crublet; Pavel Macek; Rime Kerfah; Diego F. Gauto; Jérôme Boisbouvier; Paul Schanda. "Sensitive proton-detected solid-state NMR spectroscopy of large proteins with selective CH3 labelling: application to the 50S ribosome subunit". Chemical Communications 52 61 (2016): 9558-9561. https://doi.org/10.1039%2Fc6cc04484k.
    10.1039/c6cc04484k
  16. Elias D. López; Juan Pablo Arcon; Diego F. Gauto; Ariel A. Petruk; Carlos P. Modenutti; Victoria G. Dumas; Marcelo A. Marti; Adrian G. Turjanski. "WATCLUST: a tool for improving the design of drugs based on protein-water interactions: Fig. 1.". Bioinformatics 31 22 (2015): 3697-3699. https://doi.org/10.1093%2Fbioinformatics%2Fbtv411.
    10.1093/bioinformatics/btv411
  17. Lucas A. Defelipe; Esteban Lanzarotti; Diego Gauto; Marcelo A. Marti; Adrián G. Turjanski; Rebecca C. Wade. "Protein Topology Determines Cysteine Oxidation Fate: The Case of Sulfenyl Amide Formation among Protein Families". PLOS Computational Biology 11 3 (2015): e1004051-e1004051. https://doi.org/10.1371%2Fjournal.pcbi.1004051.
    10.1371/journal.pcbi.1004051
  18. Modenutti, C.; Gauto, D.; Radusky, L.; Blanco, J.; Turjanski, A.; Hajos, S.; Marti, M.A.. "Using crystallographic water properties for the analysis and prediction of lectin-carbohydrate complex structures". Glycobiology 25 2 (2015): 181-196. http://www.scopus.com/inward/record.url?eid=2-s2.0-84945179902&partnerID=MN8TOARS.
    10.1093/glycob/cwu102
  19. Juan P. Bustamante; Stefania Abbruzzetti; Agnese Marcelli; Diego Gauto; Leonardo Boechi; Alessandra Bonamore; Alberto Boffi; et al. "Ligand Uptake Modulation by Internal Water Molecules and Hydrophobic Cavities in Hemoglobins". The Journal of Physical Chemistry B 118 5 (2014): 1234-1245. https://doi.org/10.1021%2Fjp410724z.
    10.1021/jp410724z
  20. Diego F Gauto; Ariel A Petruk; Carlos P Modenutti; Juan I Blanco; Santiago Di Lella; Marcelo A Martí. "Solvent structure improves docking prediction in lectin–carbohydrate complexes". Glycobiology 23 2 (2012): 241-258. https://doi.org/10.1093%2Fglycob%2Fcws147.
    10.1093/glycob/cws147
  21. Carlos M. A. Guardia; Diego F. Gauto; Santiago Di Lella; Gabriel A. Rabinovich; Marcelo A. Marti´; Dari´o A. Estrin. "An Integrated Computational Analysis of the Structure, Dynamics, and Ligand Binding Interactions of the Human Galectin Network". Journal of Chemical Information and Modeling 51 8 (2011): 1918-1930. https://doi.org/10.1021%2Fci200180h.
    10.1021/ci200180h
  22. Diego F. Gauto; Santiago Di Lella; Darío A. Estrin; Hugo L. Monaco; Marcelo A. Martí. "Structural basis for ligand recognition in a mushroom lectin: solvent structure as specificity predictor". Carbohydrate Research 346 7 (2011): 939-948. https://doi.org/10.1016%2Fj.carres.2011.02.016.
    10.1016/j.carres.2011.02.016
  23. Diego F. Gauto; Santiago Di Lella; Carlos M. A. Guardia; Dari´o A. Estrin; Marcelo A. Marti´. "Carbohydrate-Binding Proteins: Dissecting Ligand Structures through Solvent Environment Occupancy". The Journal of Physical Chemistry B 113 25 (2009): 8717-8724. https://doi.org/10.1021%2Fjp901196n.
    10.1021/jp901196n
Pré-impressão
  1. Diego F. Gauto; Olga O. Lebedenko; Lea Marie Becker; Isabel Ayala; Roman Lichtenecker; Nikolai R. Skrynnikov; Paul Schanda. "Aromatic ring flips in differently packed ubiquitin protein crystals from MAS NMR and MD". 2022. https://doi.org/10.1101/2022.07.07.499110.
    10.1101/2022.07.07.499110

Outros

Outra produção
  1. Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex. 2018. Diego Gauto; Leandro Estrozi; Charles Schwieters; Gregory Effantin; Pavel Macek; remy sounier; Astrid C. Sivertsen; et al. https://doi.org/10.1101/498287.
    10.1101/498287
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2019/06/05 Enhancing the carbohydrate protein binding site: Selective NMR dynamic nuclear polarization International workshop, on the future of the glycosciences?
(Grenoble, França)
2019/01/20 Selective DNP enhanced NMR of carbohydrate protein binding sites Franco-Israeli symposium in magnetic resonance
weizmann institute, Rehovot, Israel (Rehovot, Israel)
2018/09/05 Structure determination of a half-megadalton aminopeptidase enzyme complex from an integrated NMR and cryo-EM approach CCPN 2018 Conference
Kent University, Canterbury, England (Reino Unido)
2017/09/10 Structure and functional dynamics of a half-megadalton enzyme assembly from solid-state NMR 10th Alpine conference on solid state NMR
(Chamonix, França)
2017/03/06 Functional enzyme dynamics by solid state NMR Advance isotopic labeled methods for integrative structural biology conference (AILM)In
Institut de Biologie Structurale (Grenoble, França)
Distinções

Prémio

2019 Prix de la Publication « Jeune Chercheur 2018-2019
Institut de Biologie Structurale, França