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Ana M Melo completed her PhD at IST (University of Lisbon, Portugal) in January 2014. Her PhD research was focused on the elucidation of the key factors that govern the formation of amyloid-like fibers of non-amyloidogenic proteins catalyzed by anionic lipid membranes. It resulted in 5 first author publications (BBA Biomembr, J. Phys. Chem. B, Soft Matter, Phys. Chem. Chem. Phys., and Methods Mol. Biol). After finishing her PhD, AM Melo immediately moved to Yale University (USA) to join Rhoades Lab, a world leader group in the application of smFRET for studying function/dysfunction of several intrinsically disordered amyloidogenic proteins. She worked as a postdoc under the supervision of Prof. Elizabeth Rhoades at 2 prestigious American Ivy League Universities (Yale and University of Pennsylvania -UPenn) (Feb 2014-Sept 2017). At Yale, she also worked in an ambitious collaborative project with Prof. Scott Holley to apply FCCS in live zebrafish embryos. In July 2015, she moved to UPenn with Prof. Rhoades. During this transition, she acquired experience in setting-up a new lab; specifically, in building home-made microscopes for smFRET measurements. In addition, she received a Postdoc Fellowship from the NSF Center for Engineering MechanoBiology (Jan-Sept 2017). Her work at Rhoades Lab was primarily focused on exploring the conformational plasticity of tau protein bound to soluble tubulin using smFRET, and its role in tau function/dysfunction. Overall her research work in Rhoades Lab resulted in 3 high impact factor publications: (i) first author in a PNAS paper (2016-recommended in F1000 Prime), (ii) first author in Methods in Cell Biology (2017), and finally (iii) co-author in a Dev. Cell paper (2015) from the collaboration with Holley Lab. She also mentored undergraduate students (at Yale and UPenn) and 3 first-year PhD rotation students at UPenn. Her work attained international recognition with several selected/invited presentations (including US Biophysical meetings (2016 and 2017) and a Gordon Research Seminar (2016) of Intrinsically Disordered Proteins. She was also awarded with a CPOW Travel Grant from BPS that recognizes promising Young Women in Biophysics and was co-chair of 2016 IDPs Platform section at BPS Meeting (Los Angeles). AM Melo returned to Portugal in Oct 2017 to pursue her scientific independence. Currently, AM Melo is a CEEC-individual researcher (Junior level). She is Head of the Bioimaging Facility at IST (member of PPBI and EuroBioimaging) and Invited Assistant Professor at the Dept. of Bioengineering at IST. The major goals of her team are: (i) the development of innovative single-molecule approaches to characterize the early-events of protein aggregation, and (ii) apply advanced fluorescence methods to study lipid membranes organization. AM Melo already obtained 3 Grants (2-ongoing) as Principal Investigator (PI) over the last 5-years (total ~ 318.2K Eur): 1. FCT Grant for huntingtin exon-1 (Huntington´s disease); 2. FCT Exploratory Grant for tau protein; 3. Maria de Sousa Award (by Bial Foundation/ PT Medical Association). She built the first smFRET microscope in Portugal. AM Melo is also Management Committee Member of the COST Action ML4NGP. She supervised 2 Postdocs and 7 Master Students (3-ongoing). Part of AM Melo work in Portugal has already been published: Front Mol Neurosci. (2020, corresponding author), Int. J. Mol. Sci. (2021, co-author), Langmuir (2021, co-corresponding author) and Pharmaceutics (2022, co-author). She was also invited/selected for 5 Oral International Talks (e.g. Biophysics Meetings from US, Iberian and Brazil; and from International Society of Neurochemistry); and also at 7 national presentations (e.g. invited 2021 PT Neuroscience Meeting).
Identificação

Identificação pessoal

Nome completo
Ana Margarida Pereira de Melo

Nomes de citação

  • Ana M. Melo
  • Melo, A.M.
  • Melo, Ana M.

Identificadores de autor

Ciência ID
A614-4619-1CCF
ORCID iD
0000-0002-2086-5939
Researcher Id
Q-6580-2017

Endereços de correio eletrónico

  • anammelo@tecnico.ulisboa.pt (Profissional)

Telefones

Telefone
  • 218419219 (Profissional)

Moradas

  • Instituto Superior Técnico, Institute for Bioengineering and Biosciences (iBB), Complexo Interdisciplinar, Av. Rovisco Pais, 1049-001 , Lisbon, Lisboa, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Biofísica
  • Ciências Naturais - Ciências Biológicas - Bioquímica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Francês Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Formação
Grau Classificação
2010/01/01 - 2014/01/16
Concluído
PhD Chemistry (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Lipid-protein interaction in “Amyloid-like” fiber formation" (TESE/DISSERTAÇÃO)
Pass with Distinction
2009/10/29
Concluído
Biochemistry (Mestrado)
Especialização em Medical Biochemistry
Universidade de Lisboa Faculdade de Ciências, Portugal
"The Role of Lipid Membranes on the Molecular Mechanism of Amyloid-like Fibril Formation" (TESE/DISSERTAÇÃO)
18/20
2007/07/31
Concluído
Biochemistry (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"N/A" (TESE/DISSERTAÇÃO)
17/20
Percurso profissional

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento
Empregador
2021/06/01 - Atual Professor Auxiliar Convidado (Docente Universitário) Universidade de Lisboa Instituto Superior Técnico, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2019/06/01 - Atual Junior Researcher under the CEEC-Individual FCT call (Independent Support, 1st Edition) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
2017/10/27 - 2019/05/31 Postdoctoral Researcher Fellow under the Portuguese Platform of BioImage (PPBI) Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/07/01 - 2017/09/15 Postdoctoral Researcher Fellow (Rhoades Lab) University of Pennsylvania Department of Chemistry, Estados Unidos
2014/02/01 - 2015/06/30 Postdoctoral Associate (Rhoades Lab) Yale University Department of Molecular Biophysics and Biochemistry, Estados Unidos
2010/01/01 - 2014/01/16 PhD Student Universidade de Lisboa Instituto Superior Técnico, Portugal
2008/09/01 - 2009/09/30 Master Student Training Universidade de Lisboa Instituto Superior Técnico, Portugal
Projetos

Bolsa

Designação Financiadores
2023/01/01 - 2024/12/31 Tracking the unconventionally secreted tau species: insights into the progression of tauopathies
45/2022
Investigador responsável
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
BIAL Foundation and Portuguese Medical Association
Em curso
2023/03/01 - 2024/08/31 The role of the plasma membrane in the unconventional secretion of tau: insights into the progression of tauopathies
2022.01454.PTDC
Investigador responsável
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2018/10/01 - 2022/09/30 The aggregation-prone structures of Huntingtin exon 1 at the single-molecule level: Influence of membranes and implications in Huntington disease
PTDC/BIA-BFS/30959/2017
Investigador responsável
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2016/07/15 - 2017/09 Molecular mechanisms and cellular implications of tau dysfunction
info:eu-repo/grantAgreement/NIH/NATIONAL INSTITUTE ON AGING/3RF1AG053951-01S1/US
Investigador
University of Pennsylvania School of Arts and Sciences, Estados Unidos
National Institutes of Health
Concluído
2013/06/01 - 2016/11/30 MULTIFUNCTIONAL NANOMATERIALS FOR FLUORESCENCE IMAGING AND SENSING
RECI/CTM-POL/0342/2012
Investigador
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2013/07/01 - 2015/12/31 Modulation of Calcitonin Amyloid Formation by Lipid Membranes and Extracellular Matrix: Implications for the Mechanism of Cytotoxicity of Amyloidogenic Polypeptides
PTDC/BBB-BQB/2661/2012
Investigador
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/01/01 - 2013/06/30 Amyloidogenic proteins: role of lipid-protein interaction in amyloid-like fibril formation
PTDC/QUI-BIQ/099947/2008
Investigador
Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluído

Projeto

Designação Financiadores
2019/06/01 - 2025/05/31 Single-molecule characterization of membrane-binding of Huntingtin protein: implications in Huntington Disease
CEECIND/00884/2017
Investigador responsável
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2017/01 - 2017/09 The role of tau point-mutations in function (tau-tubulin complex)
CMMI-1548571
Bolseiro de Pós-Doutoramento
University of Pennsylvania, Estados Unidos
NSF Center for Engineering MechanoBiology (CEMB)
Concluído

Outro

Designação Financiadores
2020/02 - 2021/09 The Portuguese Platform of Bioimage, Head of the Advanced Fluorescence Microscopy Facility at iBB
POCI-01-0145-FEDER-022122
Fundação para a Ciência e a Tecnologia
Produções

Publicações

Artigo em revista
  1. Cartaxo, Ana Luísa; Fernandes, Ana; Rodrigues, Carlos; Melo, Ana M.; Tecklenburg, Katja; Margreiter, Eva; Day, Richard M; da Silva, Cláudia L; Cabral, Joaquim M S. "Developing a cell-microcarrier tissue engineered product for muscle repair using a bioreactor system". Tissue Engineering Part C: Methods (2023): http://dx.doi.org/10.1089/ten.tec.2023.0122.
    10.1089/ten.tec.2023.0122
  2. Miranda, André; Lopez-Blanco, Roi; Lopes-Nunes, Jessica; Melo, Ana M.; Campello, Maria P. C. ; Paulo, Antonio; Oliveira, Maria C. ; Mergny, Jean-Louis; Oliveira, Paula A. ; Fernandez-Megia, Eduardo; Cruz, Carla. "Gallic Acid-Triethylene Glycol Aptadendrimers Synthesis, Biophysical Characterization and Cellular Evaluation". Pharmaceutics 14 11 (2022): 2456. http://dx.doi.org/10.3390/pharmaceutics14112456.
    10.3390/pharmaceutics14112456
  3. Scanavachi, Gustavo; Coutinho, Ana; Fedorov, Alexander Andreevich; Prieto, Manuel; Melo, Ana M.; Itri, Rosangela. Autor correspondente: Melo, Ana M.. "Lipid Hydroperoxide Compromises the Membrane Structure Organization and Softens Bending Rigidity". Langmuir 37 33 (2021): 9952-9963. http://dx.doi.org/10.1021/acs.langmuir.1c00830 (co-Corresponding author).
    Publicado • 10.1021/acs.langmuir.1c00830
  4. Eftimov, Petar; Yokoi, Norihiko; Melo, Ana M.; Daull, Philippe; Georgiev, Georgi As.. "Interactions of Meibum and Tears with Mucomimetic Polymers: A Hint towards the Interplay between the Layers of the Tear Film". International Journal of Molecular Sciences 22 5 (2021): 2747. http://dx.doi.org/10.3390/ijms22052747.
    Publicado • 10.3390/ijms22052747
  5. Birol, Melissa; Melo, Ana M.. Autor correspondente: Melo, Ana M.. "Untangling the Conformational Polymorphism of Disordered Proteins Associated With Neurodegeneration at the Single-Molecule Level". Frontiers in Molecular Neuroscience 12 (2020): http://dx.doi.org/10.3389/fnmol.2019.00309 (Corresponding author).
    Publicado • 10.3389/fnmol.2019.00309
  6. Melo, Ana M.; Elbaum-Garfinkle, Shana; Rhoades, Elizabeth. "Insights into tau function and dysfunction through single-molecule fluorescence". Methods in Tau Cell Biology (2017): 27-44. http://dx.doi.org/10.1016/bs.mcb.2017.06.010.
    Publicado • 10.1016/bs.mcb.2017.06.010
  7. Melo, Ana M.; Coraor, Juliana; Alpha-Cobb, Garrett; Elbaum-Garfinkle, Shana; Nath, Abhinav; Rhoades, Elizabeth. "A functional role for intrinsic disorder in the tau-tubulin complex". Proceedings of the National Academy of Sciences 113 50 (2016): 14336-14341. http://dx.doi.org/10.1073/pnas.1610137113, (Recommended in F1000 Prime).
    Publicado • 10.1073/pnas.1610137113
  8. Jülich, Dörthe; Cobb, Garrett; Melo, Ana M.; McMillen, Patrick; Lawton, Andrew K.; Mochrie, Simon G.J.; Rhoades, Elizabeth; Holley, Scott A.. "Cross-Scale Integrin Regulation Organizes ECM and Tissue Topology". Developmental Cell 34 1 (2015): 33-44. http://dx.doi.org/10.1016/j.devcel.2015.05.005.
    Publicado • 10.1016/j.devcel.2015.05.005
  9. Melo, Ana M.; Fedorov, Aleksander; Prieto, Manuel; Coutinho, Ana. "Exploring homo-FRET to quantify the oligomer stoichiometry of membrane-bound proteins involved in a cooperative partition equilibrium". Phys. Chem. Chem. Phys. 16 34 (2014): 18105-18117. http://dx.doi.org/10.1039/c4cp00060a.
    Publicado • 10.1039/c4cp00060a
  10. Melo, Ana M.; Loura, Luís M. S.; Fernandes, Fábio; Villalaín, José; Prieto, Manuel; Coutinho, Ana. "Electrostatically driven lipid–lysozyme mixed fibers display a multilamellar structure without amyloid features". Soft Matter 10 6 (2014): 840-850. http://dx.doi.org/10.1039/c3sm52586d.
    Publicado • 10.1039/c3sm52586d
  11. Ana M. Melo; Prieto, Manuel; Coutinho, Ana. "Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS)". Methods in Molecular Biology 1076 (2014): 575-595.
    Publicado • 10.1007/978-1-62703-649-8_26
  12. Melo, Ana M.; Ricardo, Joana C.; Fedorov, Aleksander; Prieto, Manuel; Coutinho, Ana. "Fluorescence Detection of Lipid-Induced Oligomeric Intermediates Involved in Lysozyme “Amyloid-Like” Fiber Formation Driven by Anionic Membranes". The Journal of Physical Chemistry B 117 10 (2013): 2906-2917. http://dx.doi.org/10.1021/jp310396v.
    Publicado • 10.1021/jp310396v
  13. Melo, Ana M.; Prieto, Manuel; Coutinho, Ana. "The effect of variable liposome brightness on quantifying lipid–protein interactions using fluorescence correlation spectroscopy". Biochimica et Biophysica Acta (BBA) - Biomembranes 1808 10 (2011): 2559-2568. http://dx.doi.org/10.1016/j.bbamem.2011.06.001.
    Publicado • 10.1016/j.bbamem.2011.06.001
Poster em conferência
  1. Sousa, T.; Damas, G.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A.M.. "The flanking polyQ regions of Huntingtin exon 1 display distinct dynamic signatures in its membrane-bound state: insights into Huntington´s disease". Trabalho apresentado em 25th IUBMB Congress, the 46th FEBS Congress and the 15th PABMB Congress, 2022.
  2. ” Sousa, T.; Damas, G.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A.M.. "The flanking polyQ regions of Huntingtin exon 1 display distinct conformational signatures in its membrane­bound state: insights into Huntington´s disease". Trabalho apresentado em Gordon Research Conference (GRC) in Intrinsically Disordered Proteins, 2022.
  3. Sousa, T.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A.M.. "The conformational dynamics of the flanking polyQ regions in the membrane-bound state of huntingtin exon 1". Trabalho apresentado em 20th International Union for Pure and Applied Biophysics (IUPAB), 2021.
  4. Sousa, T.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A.M.. "Effects of the flanking polyQ regions and membrane physical properties on huntingtin binding to lipid vesicles". Trabalho apresentado em Biophysical Society 65th Annual Meeting, 2021.
  5. Scanavachi, G.; Melo, A.M.; Coutinho, A.; Fedorov, A.; Prieto, M.; Itri, R.. "The impact of oxidized POPC derivatives on lipid bilayer hydration and fluidity: insights from steady-state and time-resolved fluorescence methodologies". Trabalho apresentado em Biophysical Society Thematic Meeting: Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level, 2019.
  6. Melo, A.M.; Coraor, J.; Alpha-Cobb, G.; Elbaum-Garfinkle, S.; Rhoades, E.. "Exploring the conformational plasticity of tau: insights into function and dysfunction". Trabalho apresentado em 11th European Biophysics Congress, 2017.
  7. Melo, A.M.; Coutinho, A.; Loura, L.; Fernandes, F.; Prieto, M.. "Amyloid-like fibers and the role of lipids: multibilayer structure and protein oligomerization from FLIM-FRET and homo-FRET methodologies". Trabalho apresentado em FRET 2 International Discussion Meeting, 2016.
  8. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Oligomer stoichiometry of membrane-bound proteins involved in a cooperative partition equilibrium: a Homo-FRET study". Trabalho apresentado em Biophysical Society 59th Annual Meeting, 2015.
  9. Coraor, J.; Melo, A.M.; Cobb, G.; Rhoades, E.. "A comparison of the conformational changes of tau isoforms in the tau-tubulin complex". Trabalho apresentado em Biophysical Society 59th Annual Meeting, 2015.
  10. Melo, A.M.; Cobb, G.; Coraor, J.; Elbaum-Garfinkle, S.; Rhoades, E.. "Single-molecule FRET reveals an extended structure of tau bound to tubulin heterodimers". Trabalho apresentado em Biophysical Society 59th Annual Meeting, 2015.
    10.1364/fio.2005.fwk6
  11. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "A Homo-FRET-based method for quantifying the oligomer stoichiometry of membrane-bound proteins involved in a cooperative partition equilibrium”". Trabalho apresentado em XIVth Congress of the Spanish Biophysical Society, 2014.
  12. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Tracking membrane-driven protein oligomerization using fluorescence lifetime and homo- FRET studies". Trabalho apresentado em 9th European Biophysics Congress, 2013.
  13. Melo, A.M.; Loura, L.M.S.; Villalaín, J.; Fernandes, F.; Prieto, M.; Coutinho, A.. "Biophysical features of electrostatically-driven lipid-protein fibers". Trabalho apresentado em 9th European Biophysics Congress, Lisbon, 2013.
  14. Melo, A.M.; Loura, L.M.S.; Villalaín, J.; Fernandes, F.; Prieto, M.; Coutinho, A.. "Non-amyloidogenic lipid-protein fibers display a multilamellar structure: implications on lipid/protein dynamics and membrane surface dehydration". Trabalho apresentado em 27th Annual Symposium of the Protein Society, 2013.
  15. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Amyloid-like fibril formation driven by anionic lipid membranes: multiparametric fluorescence detection of lysozyme oligomeric intermediates". Trabalho apresentado em 22nd IUBMB & 37th FEBS Congress, 2012.
  16. Melo, A.M.; Coutinho, A.; Prieto, M.. "Quantifying protein binding to lipid vesicles using fluorescence correlation spectroscopy (FCS)". Trabalho apresentado em Biophysical Society 55th Annual Meeting (USA), 2011.
  17. Melo, A.M.; Prieto, M.; Coutinho, A.. "Lipid-protein interaction and amyloid-like fiber formation: FLIM and FCS studies". Trabalho apresentado em 8th European Biophysics Congress, 2011.
  18. Coutinho, A.; Melo, A.M.; Prieto, M.. "Lipid-protein interaction: quantifying protein binding to lipid vesicles using single colour fluorescence correlation spectroscopy (FCS)". Trabalho apresentado em IV Spanish Portuguese Biophysical Congress, 2010.
  19. Coutinho, A.; Melo, A.M.; Loura, L.M.S.; Prieto, M.. "Influence of protein surface coverage on lysozyme interaction with anionic lipid vesicles". Trabalho apresentado em 7th European Biophysics Congress, 2009.
  20. Melo, A.M.; Coutinho, A.; Prieto, M.. "Fluorescence correlation spectroscopy studies of lysozyme partition to phospholipid vesicles". Trabalho apresentado em 7th European Biophysics Congress, 2009.
Resumo em conferência
  1. Sousa, T.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A.M.. "The conformational dynamics of the flanking polyQ regions in the membrane-bound state of huntingtin exon 1". Trabalho apresentado em Biophysical Society 66th Annual Meeting, Biophys. J., San Francisco, 2022.
    Publicado
  2. Sousa, T.; Damas, G.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A.M.. "The flanking polyQ regions of Huntingtin exon 1 display distinct dynamic signatures in its membrane-bound state: insights into Huntington´s disease". Trabalho apresentado em FEBS Open Bio, Lisbon, 2022.
    Publicado
  3. Sousa, T.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A.M.. "Effects of the flanking polyq regions and membrane physical properties on huntingtin binding to lipid vesicles". Trabalho apresentado em Biophysical Society 65th Annual Meeting, Biophys. J., 2021.
    Publicado
  4. Ricardo J.C.; Scanavachi, G.; Fernandes, F.; Melo, A.M.; Itri, R.; Coutinho, A.; Prieto, M.. "Membrane remodeling by the amyloidogenic peptide IAPP: surface crowding relevance". Trabalho apresentado em Joint 12th EBSA / 10th ICBP-IUPAP Biophysics Congress, Eur. Biophys. J., Madrid, 2019.
    Publicado
  5. Melo, A.M.; Rhoades, E.. "Exploring the functional and structural impact of disease-associated mutants of tau". Trabalho apresentado em Biophysical Society 61st Annual Meeting, Biophys. J., New Orleans, 2017.
    Publicado
  6. Melo, A.M.; Coraor, J.; Alpha-Cobb G.; Elbaum-Garfinkle, S.; Rhoades, E.. "Exploring the conformational plasticity of tau: insights into function and dysfunction". Trabalho apresentado em 11th European Biophysics Congress, Eur. Biophys. J., Edinburgh, 2017.
    Publicado
  7. Melo, A.M.; Cobb, G.; Coraor, J.; Elbaum-Garfinkle, S.; Rhoades, E.. "Determining a topological model for tau bound to tubulin heterodimers". Trabalho apresentado em Biophysical Society 60th Annual Meeting, Biophys. J., Los Angeles, 2016.
    Publicado
  8. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Oligomer stoichiometry of membrane-bound proteins involved in a cooperative partition equilibrium: a Homo-FRET study". Trabalho apresentado em Biophysical Society 59th Annual Meeting, Biophys. J., Baltimore, 2015.
    Publicado • 10.1603/ice.2016.115877
  9. Coraor, J.; Melo, A.M.; Cobb, G.; Rhoades, E.. "A comparison of the conformational changes of tau isoforms in the tau-tubulin complex". Trabalho apresentado em Biophysical Society 59th Annual Meeting, Biophys. J., Baltimore, 2015.
    Publicado
  10. Melo, A.M.; Cobb, G.; Coraor, J.; Elbaum-Garfinkle, S.; Rhoades, E.. "Single-molecule FRET reveals an extended structure of tau bound to tubulin heterodimers". Trabalho apresentado em Biophysical Society 59th Annual Meeting, Biophys. J., Baltimore, 2015.
    Publicado
  11. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Tracking membrane-driven protein oligomerization using fluorescence lifetime and homo-FRET studies". Trabalho apresentado em 9th European Biophysics Congress, Eur. Biophys. J., Lisbon, 2013.
    Publicado
  12. Melo, A.M.; Loura, L.M.S.; Villalaín, J.; Fernandes, F.; Prieto, M.; Coutinho, A.. "Biophysical features of electrostatically-driven lipid-protein fibers". Trabalho apresentado em 9th European Biophysics Congress, Eur. Biophys. J., Lisbon, 2013.
    Publicado
  13. Melo, A.M.; Loura, L.M.S.; Villalaín, J.; Fernandes, F.; Prieto, M.; Coutinho, A.. "Non-amyloidogenic lipid-protein fibers display a multilamellar structure: implications on lipid/protein dynamics and membrane surface dehydration". Trabalho apresentado em 27th Annual Symposium of the Protein Society, Protein Sci., Boston, 2013.
    Publicado
  14. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Exploring fluorescence lifetime and homo- FRET measurements to monitor lysozyme oligomerization in anionic lipid membranes: relation to “amyloid-like” fibril formation". Trabalho apresentado em Biophysical Society 56th Annual Meeting, Biophys. J., San Diego, 2012.
    Publicado
  15. Melo, A.M.; Fedorov, A.; Prieto, M.; Coutinho, A. "Amyloid-like” fibril formation driven by anionic lipid membranes: multiparametric fluorescence detection of lysozyme oligomeric intermediates". Trabalho apresentado em 22nd IUBMB & 37th FEBS Congress, FEBS J., Sevilla, 2012.
    Publicado
  16. Melo, A.M.; Prieto, M.; Coutinho, A.. "Quantifying protein binding to lipid vesicles using Fluorescence Correlation Spectroscopy (FCS)". Trabalho apresentado em Biophysical Society 55th Annual Meeting, Biophys. J., Baltimore, 2011.
    Publicado
  17. Melo, A.M.; Prieto, M.; Coutinho, A.. "Lipid-protein interaction and amyloid-like fiber formation: FLIM and FCS studies". Trabalho apresentado em 8th European Biophysics Congress, Eur. Biophys. J., Budapest, 2011.
    Publicado
  18. Coutinho, A.; Melo, A.M.; Loura, L.M.S.; Prieto, M.. "Influence of protein surface coverage on lysozyme interaction with anionic lipid vesicles". Trabalho apresentado em 7th European Biophysics Congress, Eur. Biophys. J., Genoa, 2009.
    Publicado
  19. Melo, A.M.; Coutinho, A.; Prieto, M.. "Fluorescence correlation spectroscopy studies of lysozyme partition to phospholipid vesicles". Trabalho apresentado em 7th European Biophysics Congress, Eur. Biophys. J., Genoa, 2009.
    Publicado
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2022 The conformational dynamics of the flanking polyQ regions in the membrane-bound state of huntingtin exon 1 Biophysical Society 66th Annual Meeting
(San Francisco, Estados Unidos)
2022 Untangling the role of disordered proteins in neurodegeneration at the single-molecule level (Invited) Workshop Neurodegenerative diseases RedeSAÚDE ULisboa
(Lisbon, Portugal)
2022 Förster Resonance Energy Transfer - FRET, Fluorescence Lifetime Imaging - FLIM and Single-molecule methods (Invited) 13th Course on Optical Microscopy Imaging for Biosciences
(Porto)
2021 HUNTINGTIN EXON 1 BINDING TO LIPID VESICLES: the role of membrane physical properties and the flanking polyQ regions (Invited) XVII Meeting of the Portuguese Society for Neuroscience
(Coimbra, Portugal)
2019 Membrane remodeling by the amyloidogenic peptide IAPP: surface crowding relevance Joint 12th EBSA / 10th ICBP-IUPAP Biophysics Congress
(Madrid, Espanha)
2019 Untangling the conformational plasticity of tau through single-molecule FRET Strategies and tools for modulating pathologic protein self-assembly
(Porto, Portugal)
2019 Exploring the conformational plasticity of tau at the single-molecule level Integrative Approaches to Protein Folding & Aggregation
(Lisbon, Portugal)
2019 Untangling the role of disordered proteins in neurodegeneration at the single-molecule level (Invited)
Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen (Países Baixos)
2018 Exploring the conformational plasticity of tau by single-molecule FRET: Insights into its biological function and dysfunction Chemistry at ULisboa (3ECQUL)
(Lisbon, Portugal)
2018 Determining a topological model for tau bound to tubulin heterodimers by smFRET: Insights into its function/dysfunction VIth Jornadas Ibéricas de Fotoquímica (6'JIF)
(Aveiro, Portugal)
2018 Exploring the conformational plasticity of tau-tubulin complex by single-molecule FRET: insights into function of a dysfunctional protein (Invited) 6th Iberian / 10th Iberoamerican Biophysics Congress
(Castellón, Espanha)
2017 Proteins on membrane interfaces: Structure and dynamics of lipid-protein fibers from advanced FRET methodologies and microscopy VII Iberian Meeting on Colloids and Interfaces (RICI-VII)
(Madrid, Espanha)
2017 Exploring the functional and structural impact of disease-associated mutants of tau Biophysical Society 61st Annual Meeting,
(New Orleans, Estados Unidos)
2016 Conformational plasticity in the tau-tubulin complex: A functional role for intrinsic disorder (Invited)
i3S - Instituto de Investigação e Inovação, University of Porto (Porto, Portugal)
2016 Determining a topological model for tau bound to tubulin heterodimers Biophysical Society 60th Annual Meeting
(Los Angeles, Estados Unidos)
2016 Exploring the conformational plasticity of tau: insights into function and dysfunction Gordon Research Seminar (GRS) in Intrinsically Disordered Proteins
(Les Diablerets, Suiça)
2015 Lipid-protein supramolecular complexes: structure and dynamics of “amyloid- like” fibers WE-Heraeus Seminar: Avanced microscopy of membrane biophysics
(Bad Honnef, Alemanha)
2015 Quantifying the membrane assembly of amphitropic proteins by homo-FRET analysis Biophysics of proteins and surfaces: Assembly, Activation, Signaling
(Madrid, Espanha)
2015 Amyloid-like fibers and the role of lipids: Structure and dynamics from advanced FRET methodologies and microscopy Latin American Crosstalk in Biophysics
(Salto, Uruguai)
2014 Structure and dynamics of amyloid-like protein fibers: FCS, FRET, FLIM, and FRAP XIV Iberian Peptide Meeting
(Bilbao, Espanha)
2013 Structure and dynamics of “amyloid-like” lipid-protein fibers: FCS, FRET, FLIM and FRAP study 45th American Chemical Society National Meeting
(New Orleans, Estados Unidos)
2013 Amyloid-like fibers formed upon interaction of proteins and anionic lipids: Structure and dynamics from advanced FRET methodologies and microscopy The Iberian Membrane Biophysics Colloquium
(San Sebastian, Espanha)
2013 Lipid-protein interactions in "amyloid-like" fiber formation
European Molecular Biology Laboratory (EMBL) (Heidelberg, Alemanha)
2013 Lipid-protein interactions in "amyloid-like" fiber formation
Yale University (New Haven, Estados Unidos)
2012 Coupled lysozyme partition/oligomerization triggered by anionic lipid vesicles: a time-resolved fluorescence study XIII Iberian Peptide Meeting
(Alicante, Espanha)
2012 Exploring fluorescence lifetime and homo-FRET measurements to monitor lysozyme oligomerization in anionic lipid membranes: relation to “amyloid-like” fibril formation Biophysical Society 56th Annual Meeting
(San Diego, Estados Unidos)
2012 Fluorescence and microscopy in membrane biophysics: rafts, ceramide and amyloids in lipidprotein interaction XV International Symposium on Luminescence Spectrometry
(Barcelona, Espanha)
2012 Amyloid-like fiber formation driven by lipid-protein interaction: Structure and dynamics from homo-FRET, FLIM and FCS Lipid- Protein Interactions in Membranes: Implications for Health and Disease
(Hyderabad, Índia)
2011 Advanced FRET methodologies in membrane biophysics 3th Iberian Photochemistry Meeting
(Granada, Espanha)
2011 Gel and fluid membrane domains in interaction with proteins: membrane morphological alterations and amyloid-like fiber formation XI Congress of the Spanish Biophysical Society
(Murcia, Espanha)
2010 Amyloid-like fibers formation: the role of lipid-protein interaction 4th ISN Special Neurochemistry Conference
(Sicily, Itália)
2010 Lipid-protein interaction related to amyloid-like fibril formation International Courses on Toxicology
(Coimbra, Portugal)
2010 Lipid-protein interaction: Quantification of protein binding to lipid vesicles using single colour fluorescence correlation spectroscopy (FCS) International Courses on Toxicology 2010
(Coimbra, Portugal)
2010 Lysozyme binding to phosphatidylserine-containing membranes: implications in amyloid-like fiber formation XII Iberian Peptide Meeting
(Lisbon, Portugal)
2010 FCS: Formalisms and methodologies in lipid-protein interaction studies 10th Portuguese National Meeting on Photochemistry
(Porto, Portugal)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2023/03 - Atual Carlota de Galrinho e Silva; The effect of lipid membranes in tau aggregation
Orientador
Pharmaceutical Engineering (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/03 - Atual Catarina Pimenta; The role of pathological modifications to tau protein on its membrane binding: insights into Tauopathies and Alzheimer´s disease
Orientador
Biological Engineering (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/09 - Atual Gonçalo Damas; The role of the flanking polyQ regions of huntingtin exon 1 on lipid binding: Insights into Huntingto´s disease
Orientador
Biotechnology (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/10 - 2023/02 Vanessa Ramos; Estudo da Interação do Exão-1 da Huntingtina com Membranas Lipídicas
Orientador
Biochemistry (Mestrado)
Universidade de Lisboa Faculdade de Ciências, Portugal
2021/09 - 2022/11 Daniela Muntean; Influence of lipid membranes on huntingtin exon 1 fibrillation
Orientador
Biotechnology (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/11 - 2022/03 Cláudia Alves - Postdoctoral Scientist
Orientador
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/09 - 2021/11 Adriana Inácio; A SUMO-Fusion Strategy for studying the aggregation kinetics of mutant Huntingtin Exon 1
Orientador
Biotechnology (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/04 - 2021/05 Tânia Sousa - Postdoctoral Scientist
Orientador
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/09 - 2019/11 Andreia Pereira; Expression and purification of recombinant Huntingtin exon 1 using solubilizing fusion tags
Orientador
Biotechnology (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2022/02/19 - 2022/02/23 Co-Chair of the Platform section: Intrinsically Disordered Proteins (IDP), Biophysical Society 66th Annual Meeting, San Francisco, USA (2022)
Congresso
Biophysical Society, Estados Unidos
2019 - 2019 Organizing committee of the 2019 Open day of Microscopy at the Instituto Superior Técnico (2019) PPBI - Portuguese Platform of BioImaging, Portugal
2018 - 2018 Organizer and Chair of Lisbon Biophysics Networking Meeting: From Protein Dynamics to Membrane Biophysics, Lisbon, Portugal (sponsor by American Biophysical Society) (2018)
Simpósio
Biophysical Society, Estados Unidos
2016 - 2016 Co-Chair of the Platform section: Intrinsically Disordered Proteins (IDP) and Aggregates I, Biophysical Society 60th Annual Meeting, Los Angeles, USA (2016)
Congresso
Biophysical Society, Estados Unidos
2010 - 2010 XII Iberian Peptide Meeting, Lisbon, Portugal (2010)
Congresso (Membro da Comissão Organizadora)

Júri de grau académico

Tema
Tipo de participação
Nome do candidato (Tipo de grau)
Instituição / Organização
2022/07/18 Unraveling structural dynamics of Tir-Host interactions blocking immune response
Arguente
Marta Filipa Mota Vieira (Doutoramento)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2022/03/18 Biophysical analysis of Alzheimer's Amyloid-ß peptide assembly and clearance pathways
Arguente principal
José Pedro Santos Leite (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/01/14 Investigating enzymatically controlled substrate delivery for nanopore protein sequencing
Arguente principal
Hélder David Bandarra Tavares (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Comissão de avaliação

Descrição da atividade
Tipo de assessoria
Instituição / Organização Entidade financiadora
2023/01 - 2023/02 Evaluation of research proposals
Avaliador
Dutch Research Council, Países Baixos Dutch Research Council

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2021/03 - Atual Biomolecular Engineering, Teaching Protein Structure and Folding / Protein Engineering, Responsible for 40% of all Theoretical and Practical Classes Master in Bioengineering and Nanosystems (MBioNano); Master in Biotechnology; Biological Engineering; Biomedical Engineering (Mestrado) Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 - Atual Structural Biology (Teaching an invited seminar of single-molecule techniques each academic year) Master Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/04 - 2020/04 Biomolecular Engineering (Teaching an invited seminar of single-molecule techniques) Master Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/12/18 - 2019/12/18 Molecular Biotechnology (Teaching an invited seminar about neurodegenerative diseases) Master (Biotechnology) Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/12/12 - 2019/12/12 Genetic Engineering ( Teaching an invited seminar about neurodegenerative diseases) (Mestrado integrado) Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/01/09 - 2019/01/09 Pharmaceutical Biophysics (Teaching an invited seminar of single-molecule techniques) FCT PhD program of Medicines and Pharmaceutical Innovation (i3DU) (Doutoramento) Universidade de Lisboa Faculdade de Farmácia, Portugal
2017 - 2019 Complements of Analytical Biochemistry (Teaching an invited seminar of single-molecule techniques each academic year) Licenciatura Universidade de Lisboa Faculdade de Ciências, Portugal
2017 - 2017 Biochemistry of Neurodegeneration (Teaching an invited seminar of single-molecule techniques) Master (Biochemistry) Universidade de Lisboa Faculdade de Ciências, Portugal

Entrevista (jornal / revista)

Descrição da atividade Jornal / Forum
2022/11 Cinco jovens cientistas distinguidos com o Prémio Maria de Sousa Publico
2016 Penn Chemists Uncover New Information About a Protein Linked to Alzheimer’s PennToday

Entrevista / Programa (rádio / tv)

Programa Tema
2022/11 - 2022/11 RTP Notícias Prémio Maria de Sousa. Valor de 150 mil euros para cinco jovens cientistas

Membro de associação

Nome da associação Tipo de participação
2013 - Atual Protein Society (US) Member
2011 - Atual Biophysical Society, United States Member

Tutoria

Tópico Nome do aluno
2019/07 - 2019/09 Expression and purification of recombinant Huntingtin exon 1 using a SUMO-tag Miguel Ferreira, Undergraduate student at FCUL, ULisboa
2019/02 - 2019/07 Expression and purification of recombinant Huntingtin exon 1 using an intein-tag Rafaela Cruz, Undergraduate student at FCUL, ULisboa
2018/02 - 2018/07 Studying the interaction of Calcitonin with heparin and lipid membranes Miguel Mira, Undergraduate student at FCUL, ULisboa
2017/05 - 2017/07 Understading binding between tau and neuronal tubulin Richard Potter, undergraduate student at University of Pennsylvania
2016/03 - 2016/07 Studying the C-terminal domain of the troponin I subunit (TnIC) through single-molecule FRET Sanju Wickramasinghe, 1st year graduate student at University of Pennsylvania (Lab rotation)
2016 - 2016 Studying the C-terminal domain of the troponin I subunit (TnIC) through single-molecule FRET Sunbin Deng, 1st year graduate student at University of Pennsylvania (Lab rotation)
2015/09 - 2015/12 Studying tau-tubulin interaction through single-molecule techniques Buyan Pan, 1st year graduate student at University of Pennsylvania (Lab rotation)
2014/02 - 2015/06 Determining a Topological Model for TAU Bound to Tubulin Heterodimers Juliana Coraor, undergraduate student at Yale Univeisty
Distinções

Prémio

2022 2022 BPS Travel Award for participation in the Biophysical Society 66th Annual Meeting
Biophysical Society US, Estados Unidos
2022 Maria de Sousa Award - 2nd edition
BIAL Foundation and Portuguese Medical Association, Portugal
2019 Researcher CEEC-individual FCT call (CEECIND/00884/2017) - Until 6- years contract
Fundação para a Ciência e a Tecnologia, Portugal
2017 Postdoc Research Fellowship from NSF Center for Engineering MechanoBiology (CEMB)
2016 CPOW travel award to support women in Biophysics (Biophysical Society US)
Biophysical Society US, Estados Unidos
2013 Biophysical Society (USA) Student Poster Award at the 2013 EBSA Conference
2011 International Travel Awards for participation in the Biophysical Society 55th Annual Meeting
Biophysical Society US, Estados Unidos
2010 FCT Graduate Research Fellowship (SFRH / BD / 61723 / 2009)
Fundação para a Ciência e a Tecnologia, Portugal

Outra distinção

2016 Honorary Mention to promote PhD thesis from Portuguese Chemical Society.
2013 Fellowship to participate in the 27th Annual Symposium of The Protein Society
2012 EBSA Bursary for attendance at Iberian Peptide Meeting (EPI 2012)
2012 IUBMB/FEBS/SEBBM bursary for attendance at 22nd IUBMB & 37th FEBS Congress
2010 FEBS Youth Travel Fund (YTF) to participate in a FEBS Advanced Course
2009 Fellowships for participation in the 7th European Biophysics Congress