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Mário Manuel da Silva Leite Sousa graduated in Medicine at Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto (ICBAS-UP)/Hospital Geral de Santo António (1985). I had the highest high-school and medical school classification, having received a university scholarship from Calouste Gulbenkian Foundation and medical prizes from the Hospital and Engenheiro António Almeida Foundation. I got a PhD in Reproductive Biology and Biochemistry (ICBAS-1992), did the military service (1991-1992), and went to France to perform a medical subspecialization in laboratorial reproductive medicine (American Hospital of Paris, 1993-1995), and a post-doctorate in Reproductive Biology and Physiology (INSERM U355, Clamart). I received an INSERM scholarship. I was appointed head of research at Depart. of Genetics (Dir. Prof. Alberto Barros), Faculty of Medicine of Porto (FMUP), placing it as a genetic diagnostics reference centre (1996-2008); head of research at the private Centre of Reproductive Genetics Prof. Alberto Barros (CGR), placing it as a national and international reference IVF centre (1996-2008); and head of research at the private IVF Bernabeu Institute, Alicante, Spain (1997-2001). My academic career at ICBAS started as a medical student (monitor, 1983) being currently full professor (2005), Ruler (of Cell Biology, to the integrated master's degree in medicine and veterinary medicine, and of Reproductive Medicine to the integrated master's degree in medicine (1995-), Director of Lab. of Cell Biology (1998-), and Scientific Council member (1991-2009; 2023-). I am founding member of UMIB-Multidisciplinary Biomedical Research Unit and at UMIB head of research of the group Biology and genetics of reproduction/Molecular and cellular medicine) (1994-). My scientific field is translational medicine in the areas of reproductive medicine, oncofertility, and ciliopathies. I have participated in 31 research projects (13 as PI). I authored/co-authored a total of 584 publications (496 International + 88 National), with 10992 citations, an h index of 50, and an i10 index of 191, and a total of 860 communications (299 International and 561 National). I participated in the discovery of new pathologic variants in infertile patients and in patients with Primary ciliary dyskinesia (12, in gene databases). I supervised/co-supervised 8 post-doctorate, 32 PhD and 46 MSc students, besides multiple of pregraduate students. I participated in 107 Scientific Juries, being author/co-author in 66 Prizes and Nominations and member of one international patent (WIPO PCT/IB2018/055715). I was co-responsible for discovering the mechanism of ICSI (first babies in France), which allowed the application of the technique throughout the world, responsible for discovering the mechanism of human oocyte activation, co-responsible for creating the ROSI technique (first babies in the world: microinjection of immature germ cells), responsible for the first in-vitro maturation of immature human germ cells into spermatozoa, responsible for the creation of the national preimplantation genetic testing centre (first application to familial amyloidotic polyneuropathy in Portugal and Spain), and responsible for the creation of the integrated diagnostics unit for ciliopathies, carrying out external diagnosis for hospitals in the northern region of Portugal. Besides other clinical discoveries, multiple media interviews, interventions near schools, patients and clinicians, I contributed politically to the implementation of the actual assisted reproductive system in Portugal, the cord blood bank, the gamete bank, and the programme for fertility preservation.
Identificação

Identificação pessoal

Nome completo
Mário Manuel da Silva Leite Sousa

Nomes de citação

  • Sousa, Mário

Identificadores de autor

Ciência ID
5118-4AC0-9709
ORCID iD
0000-0002-3009-3290
Researcher Id
A-7340-2019
Scopus Author Id
7103201377

Domínios de atuação

  • Ciências Médicas e da Saúde - Ciências da Saúde
  • Ciências Naturais - Ciências Biológicas - Biologia da Reprodução
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Diagnóstico e Terapias de Base Genética
  • Ciências Médicas e da Saúde - Medicina Clínica - Sistema Respiratório
  • Ciências Naturais - Ciências Biológicas - Biologia Celular

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Francês Utilizador independente (B1) Utilizador independente (B1) Utilizador elementar (A1) Utilizador independente (B1) Utilizador elementar (A1)
Espanhol; Castelhano Utilizador independente (B2) Utilizador independente (B2) Utilizador elementar (A1) Utilizador independente (B1) Utilizador independente (B2)
Formação
Grau Classificação
1993 - 1995
Concluído
Reproductive Biology and Physiology (Pós-doutoramento)
INSERM, França
1993 - 1995
Concluído
subspecialization in laboratorial reproductive medicine (Especialização pós-licenciatura)
Hôpital Américain de Paris, França
1992
Concluído
Ciências Biomédicas (Doutoramento)
Especialização em Outra:Biologia Celular
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"A Fertilização em Marthaesterias Glacialis" (TESE/DISSERTAÇÃO)
1979 - 1985
Concluído
Medicina (Mestrado integrado)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
1997 - Atual Investigador principal (carreira) (Investigação) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento
Empregador
2005/01/01 - Atual Professor Catedrático (Docente Universitário) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1998 - 2005 Professor Associado (Docente Universitário) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1990 - 1997 Professor Auxiliar (Docente Universitário) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Cargos e Funções

Categoria Profissional
Instituição de acolhimento
Empregador
2023 - Atual Member of the Scientific Council Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018 - Atual Pedagogic coordinator of Biology of the Cell unit of the course of Medicine and Veterinary Medicine Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018 - Atual Pedagogic coordinator of Reproductive Medicine unit from course of Medicine Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2014 - Atual Director de Unidade Orgânica Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2008 - Atual Responsable of transmission electron microscopy diagnostic of ciliary and platelet diseases Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1997 - Atual Director of Research: Biology and Genetics of Reproduction (BGR), Multidisciplinary Biomedical Research Unit (UMIB) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Universidade do Porto Unidade Multidisciplinar de Investigação Biomédica, Portugal
Projetos

Bolsa

Designação Financiadores
2016 - Atual Obesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity¬-related male infertility
Ref: PTDC/BIM¬MET/4712/2014
Ministério da Ciência Tecnologia e Ensino Superior
2015 - Atual Aquaporins as molecular partners of CFTR in the Blood-Testis Barrier: a crossroad between human male infertility and cystic fibrosis?”
Ref: PTDC/BBB-BQB/1368/2014
Ministério da Ciência Tecnologia e Ensino Superior
2012/04 - 2015/03 Can Hormonal (De)regulation of Ion Transporters in Human Sertoli Cells be Responsible for Infertility?
PTDC/QUI-BIQ/121446/2010
Universidade da Beira Interior, Portugal

Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluído
2007/01 - 2008/09 Gene expression profiles in stage specific male germ cells using the microarray technology Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2008/09 Genomic Imprinting in human spermatogenesis Fundação para a Ciência e a Tecnologia, I.P.
2005/10 - 2007/12 Gene expression profiles in stage specific male germ cells using the microarray technology Fundação para a Ciência e a Tecnologia, I.P.
2005/10 - 2007/12 Genomic Imprinting in human spermatogenesis Fundação para a Ciência e a Tecnologia, I.P.
2005 - 2007 Ultrastructural and cytochemical characterization of the follicular cells from cumulus oocyte complexes
FCTPOCTI/ CVT/60217/2004
Fundação para a Ciência e a Tecnologia, I.P.
2005 - 2007 Mechanisms of cell cycle control during human in vitro female meiosi
FCTPOCTI/ SAUMMO/ 59997/2004
Fundação para a Ciência e a Tecnologia, I.P.
2003 - 2005 Caracterização Electrofisiológica dos Canais Iónicos nas Células do Epitélio Seminífero
POCTI/BME/48376/02
Fundação para a Ciência e a Tecnologia, I.P.
2002 - 2005 Espermatogénese InVitro. Caracterização de Receptores de Superfície e da Expressão Genética Durante as Fases de Proliferação, Meiose e Diferenciação
POCTI/BME/43462/2001
Fundação para a Ciência e a Tecnologia, I.P.
2001 - 2004 Canais Iónicos Durante a Maturação e a Fertilização In Vitro de Ovócitos Bovinos
FCTSapiens, 35231/99
Fundação para a Ciência e a Tecnologia, I.P.
2001 - 2004 Análise de Haplotipos em Yq11.2 em Relação com Determinados Estadios da Espermatogénese
POCTI/ MGI/36363/2000
Fundação para a Ciência e a Tecnologia, I.P.
1997 - 1999 Acções da Proteína Quinase C nas Vias de Sinalização Celular Durante a Activação do Ovócito em Mamíferos
FCTPraxis XXI, PCNA/C/BIA/0100/96
Foundation for Science and Tecnology

Projeto

Designação Financiadores
2023/01/01 - Atual The effects of cryopreservation on the epigenome of human sperm.
Investigador
Universidade do Porto Faculdade de Medicina, Portugal
Fundação para a Ciência e a Tecnologia
2018/07/26 - 2021/01/21 Tracking TRAnsgenerational epigenetic Inheritance of metabolic diseases Transmitted via overweight or obese fathers
PTDC/MEC-AND/28691/2017
Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2016/04/01 - 2019/09/30 Obesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility
PTDC/BIM-MET/4712/2014
Universidade da Beira Interior, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2016/06/15 - 2018/12/14 Aquaporins as molecular partners of CFTR in the the Blood-Testis Barrier: a crossroad between human male infertility and cystic fibrosis?
PTDC/BBB-BQB/1368/2014
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/05/01 - 2013/04/30 Search for genomic structural variants in azoospermia: a study in the Portuguese population
PTDC/SAU-GMG/101229/2008
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal

Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Universidade do Porto Faculdade de Medicina, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluído
2005/10/01 - 2007/12/31 Gene expression profiles in stage specific male germ cells using the microarray technology
POCI/SAU-MMO/60709/2004
Universidade do Porto Faculdade de Medicina, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2005/10/01 - 2007/12/31 Genomic Imprinting in human spermatogenesis
POCI/SAU-MMO/60555/2004
Universidade do Porto Faculdade de Medicina, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em conferência
  1. Pereira, Sara C; Martins, Ana; Moreira, Bruno P; Bernardino, Raquel L.; Barros, Alberto; Sousa, Mário; Paiva, Sandra; Oliveira, Pedro F.; Alves, Marco G.. "Ghrelin and Leptin stimuli alter the expression of Obesity-related genes in Human Sertoli Cells". Trabalho apresentado em 12th Meeting of Young Researchers of University of Porto, 2019.
    Publicado
  2. Oliveira, J.; Pereira, R.; Santos, R.; Sousa, M.. "Homozygosity mapping using whole-exome sequencing: A valuable approach for pathogenic variant identification in genetic diseases". 2017.
    10.5220/0006248502100216
  3. Moreira, Bruno M; Martins, Ana D; Monteiro, Mariana P; Sousa, Mário; Alves, Marco G.; Oliveira, Pedro F. "Leptin modulates Sertoli cells mitochondrial function and biogenesis with implications for the nutritional support of spermatogenesis". Trabalho apresentado em 19th European Congress of Endocrinology, 2017.
    Publicado
  4. Martins, Ana D; Monteiro, Mariana P; Sousa, Mário; Barros, Alberto; Silva, Joaquina; Carvalho, Rui A; Silva, Branca M; Oliveira, Pedro F; Alves, Marco G.. "Glucagon-like peptide-1 stimulates lactate production by human Sertoli cells with possible implication to male reproductive potential". Trabalho apresentado em 50th Annual Scientific Meeting of the European Society for Clinical Investigation, 2016.
  5. Dias, Tânia R; Alves, Marco G.; Bernardino, Raquel L.; Martins, Ana D; Moreira, Ana C; Silva, Joaquina; Barros, Alberto; et al. "Caffeine and male fertility: Metabolic effects and oxidative profile in human Sertoli cells". Trabalho apresentado em IV Encontro Nacional de Pós-graduação em Ciências Biológicas, 2014.
    Publicado
  6. Oliveira, Jorge; Negrão, Luís; Fineza, Isabel; Gonçalves, Ana Rita; Froufe, Hugo; Egas, Conceição; Sousa, Mário; Santos, Rosário. "Forma rara de distrofia muscular (ligada ao gene CHKB) identificada através da sequenciação completa do exoma". 2014.
  7. Bernardino, Raquel L.; Martins, Ana D; Jesus, Tito T; Sá, Rosália; Sousa, Mário; Socorro, Sílvia; Alves, Marco G.; Oliveira, Pedro F. "Regulation by estrogens of bicarbonate secretion mechanisms in Sertoli cell". Trabalho apresentado em 37th Congress of IUPS, 2013.
    Publicado
  8. Seabra, Catarina; Quental, Sofia; Neto, Ana; Carvalho, Filipa; Gonçalves, João; Fernandes, Susana; Sousa, Mário; et al. "An Alu-mediated 1Mb deletion removes Wilms¿ tumor 1 (WT1) but not PAX6 in a patient with isolated cryptorchidism". 2013.
  9. Marau, R.; Almeida, L.; Sousa, M.; Pedreiras, P.. "A middleware to support dynamic reconfiguration of real-time networks". 2010.
    10.1109/ETFA.2010.5641306
Artigo em jornal
  1. Sousa, Mário. "Homozygoty mapping using whole-exome sequencing: a valuable approach for pathogenic variant identification in genetic diseases", 2017
    DOI: 10.5220/0006248502100216
Artigo em revista
  1. Marika Bini Antunes; Sara Pinto Cardeal; Manuel Magalhães; Emídio Vale-Fernandes; Márcia Barreiro; Rosália Sá; Mário Sousa. "Preservation of fertility in female patients with hematologic diseases". Blood Reviews (2023): http://dx.doi.org/10.1016/j.blre.2023.101115.
    10.1016/j.blre.2023.101115
  2. M R Ribeiro; A M Calado; Â Alves; R Pereira; M Sousa; R Sá. "SARS-CoV-2 tropism spectrum for human testicular cells". Human Reproduction (2023): http://dx.doi.org/10.1093/humrep/dead093.372.
    10.1093/humrep/dead093.372
  3. Tânia Santos; Ana S. Pires-Luís; Ângela Alves; Elsa Oliveira; Carla Leal; Mónica Fernandes; Emídio Vale-Fernandes; et al. "All that glitters is not gold: a stereological study of human donor oocytes". Zygote (2023): http://dx.doi.org/10.1017/s0967199423000114.
    10.1017/s0967199423000114
  4. Juan Alvarez; agustin garcia-peiro; Alberto Barros; Luís Ferraz; Sousa M; Denny Sakkas. "Double strand DNA breaks in sperm: the bad guy in the crowd". Journal of Assisted Reproduction and Genetics (2023): http://dx.doi.org/10.1007/s10815-023-02748-5.
    10.1007/s10815-023-02748-5
  5. Maria Rita Ribeiro; Ana Margarida Calado; Ângela Alves; Rute Pereira; Sousa M; Sá R. "Spatial Distribution of SARS-CoV-2 Receptors and Proteases in Testicular Cells". Journal of Histochemistry & Cytochemistry (2023): http://dx.doi.org/10.1369/00221554231168916.
    10.1369/00221554231168916
  6. Pereira, Rute; Barbosa, Telma; Cardoso, Ana Lúcia; Sá, Rosália; Sousa, Mário. "Cystic fibrosis and primary ciliary dyskinesia: Similarities and differences". Respiratory Medicine 209 (2023): 107169. http://dx.doi.org/10.1016/j.rmed.2023.107169.
    10.1016/j.rmed.2023.107169
  7. R. Pereira; V. Carvalho; C. Dias; T. Barbosa; J. Oliveira; Â. Alves; E. Oliveira; R. Sá; M. Sousa. "Characterization of a DRC1 null variant associated with primary ciliary dyskinesia and female infertility". Journal of Assisted Reproduction and Genetics 40 (2023): 765-778. https://link.springer.com/article/10.1007/s10815-023-02755-6.
    Acesso aberto • 10.1007/s10815-023-02755-6
  8. Sá R; Luís Ferraz; Alberto Barros; Sousa M. "The Klinefelter Syndrome and Testicular Sperm Retrieval Outcomes". Genes (2023): https://www.mdpi.com/2073-4425/14/3/647.
    10.3390/genes14030647
  9. Eduarda Milheiro Tinoco; Ana Rita Gigante; Edite Ferreira; Inês Sanches; Rute Pereira; Sá R; Regina Monteiro; Sousa M; Ivone Pascoal. "Primary Ciliary Dyskinesia in a Portuguese Bronchiectasis Outpatient Clinic". Genes (2023): https://www.mdpi.com/2073-4425/14/3/541.
    10.3390/genes14030541
  10. Rute Pereira; Sousa M. "Morphological and Molecular Bases of Male Infertility: A Closer Look at Sperm Flagellum". Genes (2023): https://www.mdpi.com/2073-4425/14/2/383.
    10.3390/genes14020383
  11. Sousa M. "Preimplantation genetic testing for familial amyloid polyneuropathy.". Reproductive health (2022): http://europepmc.org/abstract/med/36401333.
    10.1186/s12978-022-01491-x
  12. Ribeiro S; Sousa M. "In Vitro Fertilisation and Intracytoplasmic Sperm Injection predictive factors: A review of the effect of female age, ovarian reserve, male age, and male factor on IVF/ICSI treatment outcomes.". JBRA assisted reproduction (2022): http://europepmc.org/abstract/med/35916467.
    10.5935/1518-0557.20220000
  13. Carolina Silva; Paulo Viana; Alberto Barros; Sá R; Sousa M; Rute Pereira. "Further Insights on RNA Expression and Sperm Motility". Genes (2022): https://www.mdpi.com/2073-4425/13/7/1291.
    10.3390/genes13071291
  14. M Sousa; M Cunha; M Pereira; J Silva; A Gonçalves; P Viana; N Barros; et al. "Clinical outcomes of 127 patients with recurrent implantation failure treated with testicular sperm aspiration (TESA)". Human Reproduction (2022): http://dx.doi.org/10.1093/humrep/deac107.060.
    10.1093/humrep/deac107.060
  15. Sara Pereira; Inês F. Esperança; Soraia Pinto; Alberto Barros; Sousa M; Marco G: Alves; P.F. Oliveira. "Pregnancy Achievement by Medical Assisted Reproduction Is Correlated to the G Protein-Coupled Receptor 30 mRNA Abundance in Human Spermatozoa". Applied Sciences (2022): https://www.mdpi.com/2076-3417/12/7/3240.
    10.3390/app12073240
  16. S Mendes; Sá R; Manuel Magalhães; Franklim Marques; Sousa M; Elisabete Silva. "The Role of ROS as a Double-Edged Sword in (In)Fertility: The Impact of Cancer Treatment". Cancers (2022): https://www.mdpi.com/2072-6694/14/6/1585.
    10.3390/cancers14061585
  17. Ana Gonçalves; Ana Fortuna; Yavuz Ariyurek; Márcia Oliveira; Goreti Nadais; Jorge Pinheiro; Johan T. den Dunnen; et al. "Integrating Whole-Genome Sequencing in Clinical Genetics: A Novel Disruptive Structural Rearrangement Identified in the Dystrophin Gene (DMD)". International Journal of Molecular Sciences (2021): https://www.mdpi.com/1422-0067/23/1/59.
    10.3390/ijms23010059
  18. Barros P; Cunha M; Barros A; Sousa M; Dória S. "Clinical outcomes of 77 TESE treatment cycles in non-mosaic Klinefelter syndrome patients.". JBRA assisted reproduction (2021): http://europepmc.org/abstract/med/34751018.
    10.5935/1518-0557.20210081
  19. Sousa, Mário. "Syngamy, pronucleus, pronuclear breakdown and zygote". Asian Pac J Reprod (2021): http://www.apjr.net/text.asp?2021/10/5/239/326723.
    10.4103/2305-0500.326723
  20. M Maia; C Almeida; M Cunha; A Gonçalves; S S Soares; M Severo; C J Marques; et al. "Impact of semen parameters, sperm DNA fragmentation and sperm aneuploidy in male infertility". Human Reproduction 36 Supplement (2021): http://dx.doi.org/10.1093/humrep/deab130.041.
    10.1093/humrep/deab130.041
  21. P Barros; M Cunha; A Barros; S Dória; M Sousa. "P–038 Clinical outcomes of 77 testicular sperm extraction treatment cycles in non-mosaic Klinefelter syndrome patients". Human Reproduction 36 Supplement (2021): http://dx.doi.org/10.1093/humrep/deab130.037.
    10.1093/humrep/deab130.037
  22. Pereira, Sara C.; Esperança, Inês F; Pinto, S; Barros, Alberto; Sousa, Mário; Alves, Marco G.; Oliveira, Pedro F.. "Association of GPR30 transcript abundance in human spermatozoa with outcomes of assisted reproduction". Research@UA 2020 (2021):
    Publicado
  23. Silva JV; Santiago J; Sousa M; Oliveira PF; Fardilha M. "New evidences of ubiquitin-proteasome system activity in human sperm.". Biochimica et biophysica acta. Molecular cell research (2021): http://europepmc.org/abstract/med/33338520.
    10.1016/j.bbamcr.2020.118932
  24. Moreira BP; Silva AM; Martins AD; Monteiro MP; Sousa M; Oliveira PF; Alves MG. "Effect of Leptin in Human Sertoli Cells Mitochondrial Physiology.". Reproductive sciences (Thousand Oaks, Calif.) (2021): http://europepmc.org/abstract/med/32997289.
    10.1007/s43032-020-00328-x
  25. Marcos, Hugo; Soares Santos, Tiago; Gonçalves, Paulo; Sousa, Mário; Oliveira, Elsa; Carvalho, Carlos; Alanin, Mikkel Christian. "Primary Ciliary Diskinesia with Structuraly Normal Cilia: an unusual diagnosis". Acta Otorrinolaringológica Gallega (2020):
    Em revisão
  26. Pereira SC; Crisóstomo L; Sousa M; Oliveira PF; Alves MG. "Metabolic diseases affect male reproduction and induce signatures in gametes that may compromise the offspring health.". Environmental epigenetics (2020): http://europepmc.org/abstract/med/33324496.
    10.1093/eep/dvaa019
  27. Marques J; Carvalho F; Barros A; Sousa M. "Epimutations in human sperm from patients with impaired spermatogenesis.". Clinical epigenetics (2020): http://europepmc.org/abstract/med/33203461.
    10.1186/s13148-020-00919-0
  28. "Is Hyperglycemia the trigger for Diabetes-related Male Infertility?". European Journal of Clinical Investigation 50 S1 (2020): http://dx.doi.org/10.1111/eci.13369.
    10.1111/eci.13369
  29. Coelho S; Pires-Luís AS; Oliveira E; Alves Â; Leal C; Cunha M; Barreiro M; et al. "Stereological study of organelle distribution in human oocytes at metaphase I.". Zygote (Cambridge, England) 28 4 (2020): 308-312. http://europepmc.org/abstract/med/32285762.
    Publicado • 10.1017/s0967199420000131
  30. Lopes AC; Oliveira PF; Pinto S; Almeida C; Pinho MJ; Sá R; Rocha E; Barros A; Sousa M. "Discordance between human sperm quality and telomere length following differential gradient separation/swim-up.". Journal of assisted reproduction and genetics (2020): http://europepmc.org/abstract/med/32767207.
    10.1007/s10815-020-01897-1
  31. Carrageta DF; Guerra-Carvalho B; Sousa M; Barros A; Oliveira PF; Monteiro MP; Alves MG. "Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli.". Antioxidants (Basel, Switzerland) (2020): http://europepmc.org/abstract/med/32823893.
    10.3390/antiox9080750
  32. Nunes JS; Sousa M; Montenegro N; Matias A. "Twin Pregnancies, Crown-rump Length and Birthweight Discordancy: The Influence of Chorionicity.". Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia (2020): http://europepmc.org/abstract/med/32559796.
    10.1055/s-0040-1712128
  33. Rabaça A; Ferreira C; Bernardino R; Alves M; Oliveira P; Viana P; Barros A; Sousa M; Sá R. "Use of antioxidant could ameliorate the negative impact of etoposide on human sperm DNA during chemotherapy.". Reproductive biomedicine online (2020): http://europepmc.org/abstract/med/32376314.
    10.1016/j.rbmo.2020.01.029
  34. Braga PC; Pereira SC; Ribeiro JC; Sousa M; Monteiro MP; Oliveira PF; Alves MG. "Late-onset hypogonadism and lifestyle-related metabolic disorders.". Andrology (2020): http://europepmc.org/abstract/med/31991053.
    10.1111/andr.12765
  35. Pereira R; Oliveira J; Sousa M. "Bioinformatics and Computational Tools for Next-Generation Sequencing Analysis in Clinical Genetics.". Journal of clinical medicine (2020): http://europepmc.org/abstract/med/31947757.
    10.3390/jcm9010132
  36. Coelho, S.; Pires-Luís, A.S.; Oliveira, E.; Alves, Â.; Leal, C.; Cunha, M.; Barreiro, M.; et al. "Stereological study of organelle distribution in human oocytes at metaphase i". Zygote (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85083362126&partnerID=MN8TOARS.
    10.1017/S0967199420000131
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  247. Sousa, Mário; Sá, Rosália. "Células Estaminais Humanas". Biologia & Sociedade 2 (2006): 14-15. http://ordembiologos.pt/wp-content/uploads/2015/11/BiologiaSociedade_02.pdf.
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  248. Nuno De Magalhães Oliveira, V.; Sousa, M.; Figueiredo, H.; Costa, L.; Amaral, J.; Pereira, M.; Ferraz, L.. "Immotile cilia syndrome. Diagnostic and therapeutic approach of a rare infertility case | Síndrome dos cílios imóveis. Abordagem diagnóstica e terapêutica de um caso raro de infertilidade". Revista Internacional de Andrologia 4 1 (2006): 35-38. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547913043&partnerID=MN8TOARS.
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  250. Ribas, R.; Oback, B.; Ritchie, W.; Chebotareva, T.; Taylor, J.; Maurício, A.C.; Sousa, M.; Wilmut, I.. "Modifications to improve the efficiency of zona-free mouse nuclear transfer". Cloning and Stem Cells 8 1 (2006): 10-15. http://www.scopus.com/inward/record.url?eid=2-s2.0-33645774188&partnerID=MN8TOARS.
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  251. Fernandes, A.T.; Fernandes, S.; Gonçalves, R.; Sá, R.; Costa, P.; Rosa, A.; Ferrás, C.; et al. "DAZ gene copies: Evidence of Y chromosome evolution". Molecular Human Reproduction 12 8 (2006): 519-523. http://www.scopus.com/inward/record.url?eid=2-s2.0-33747883864&partnerID=MN8TOARS.
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  253. Oliveira, H.; Loureiro, J.; Filipe, L.; Santos, C.; Ramalho-Santos, J.; Sousa, M.; Pereira, M.d.L.. "Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis". Reproductive Toxicology 22 3 (2006): 529-535. http://www.scopus.com/inward/record.url?eid=2-s2.0-33748415574&partnerID=MN8TOARS.
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  254. Ribas, R.C.; Taylor, J.E.; McCorquodale, C.; Maurício, A.C.; Sousa, M.; Wilmut, I.. "Effect of zona pellucida removal on DNA methylation in early mouse embryos". Biology of Reproduction 74 2 (2006): 307-313. http://www.scopus.com/inward/record.url?eid=2-s2.0-31044440281&partnerID=MN8TOARS.
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  256. F Carvalho; M Sousa; J Silva; A Barros. "O¿36 Update on PGD in Portugal for familial amyloidotic polyneuropathy". Reproductive BioMedicine Online 10 (2005): 12-12. http://dx.doi.org/10.1016/s1472-6483(11)60257-5.
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  258. Calado, A.M.; Rocha, E.; Colaço, A.; Sousa, M.. "Comparative stereological study of cumulus-oocyte complexes aspirated from follicles during the estrous cycle in bovine | Estudo estereológico comparative de complexes cumulus-ovócito aspirados de folículos durante o ciclo estral em bovinos". Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 57 4 (2005): 465-475. http://www.scopus.com/inward/record.url?eid=2-s2.0-31544433939&partnerID=MN8TOARS.
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  307. Sousa, M.; Guerra, R.; Oliveira, E.; Torres, A.. "Comparative PTA staining of molluscan spermatozoa". Journal of Submicroscopic Cytology and Pathology 30 1 (1998): 183-187. http://www.scopus.com/inward/record.url?eid=2-s2.0-0003243609&partnerID=MN8TOARS.
  308. Tesarik, J.; Sousa, M.; Greco, E.; Mendoza, C.. "Spermatids as gametes: Indications and limitations". Human Reproduction 13 SUPPL. 3 (1998): 89-111. http://www.scopus.com/inward/record.url?eid=2-s2.0-17544386851&partnerID=MN8TOARS.
  309. Bernabeu, R.; Cremades, N.; Takahashi, K.; Sousa, M.. "Successful pregnancy after spermatid injection". Human Reproduction 13 7 (1998): 1898-1900. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031825879&partnerID=MN8TOARS.
    10.1093/humrep/13.7.1898
  310. Carvalho, F.; Sousa, M.; Oliveira, E.; Carvalheiro, J.; Baldaia, L.. "Ultrastructure of oogenesis in Penaeus kerathurus (Crustacea, Decapoda). I. Previtellogenic oocytes". Journal of Submicroscopic Cytology and Pathology 30 3 (1998): 409-416. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032111142&partnerID=MN8TOARS.
  311. Carvalho, F.; Sousa, M.; Oliveira, E.; Carvalheiro, J.; Baldaia, L.. "Ultrastructure of oogenesis in Penaeus kerathurus (Crustacea, Decapoda). II. Vitellogenesis". Journal of Submicroscopic Cytology and Pathology 30 4 (1998): 527-535. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038112357&partnerID=MN8TOARS.
  312. Barros, A.; Sousa, M.; Andrade, M.J.; Oliveira, C.; Silva, J.; Beires, J.. "Birth after electroejaculation coupled to intracytoplasmic sperm injection in a gun-shot spinal cord-injured man". Archives of Andrology 41 1 (1998): 5-9. http://www.scopus.com/inward/record.url?eid=2-s2.0-79961182353&partnerID=MN8TOARS.
    10.3109/01485019808988538
  313. V. Almeida; A. Barros; M. Sousa; J. Silva; E. Rocha. "P-131. Ageing, hyaluronidase removal of the cumulus and microinjection do not affect the sperm binding potential of human oocytes". Human Reproduction 12 Suppl{\_ (1997): 182-182. http://dx.doi.org/10.1093/humrep/12.suppl_2.182.
    10.1093/humrep/12.suppl_2.182
  314. Alberto Barros; Sousa M; Silva J; Almeida V; Eduardo Rocha. "Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes.". Journal of assisted reproduction and genetics (1997): https://europepmc.org/articles/PMC3454824.
    10.1007/bf02765778
  315. Sousa, M.; Barros, A.; Mendoza, C.; Tesarik, J.. "Role of Ca2+ oscillations during human preimplantation embryo development". Assisted Reproduction Reviews 7 3 (1997): 139-150. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030885179&partnerID=MN8TOARS.
  316. Sousa, M.; Barros, A.; Silva, J.; Tesarik, J.. "Developmental changes in calcium content of ultrastructurally distinct subcellular compartments of preimplantation human embryos". Molecular Human Reproduction 3 2 (1997): 83-90. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031064856&partnerID=MN8TOARS.
  317. Barros, A.; Sousa, M.; Angelopoulos, T.; Tesarik, J.. "Efficient modification of intracytoplasmic sperm injection technique for cases with total lack of sperm movement". Human Reproduction 12 6 (1997): 1227-1229. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030973284&partnerID=MN8TOARS.
    10.1093/humrep/12.6.1227
  318. Barros, A.; Silva, J.; Sousa, M.; Almeida, V.; Oliveira, C.; Beires, J.. "Pregnancy and birth after intracytoplasmic sperm injection with totally immotile sperm recovered from the ejaculate". Fertility and Sterility 67 6 (1997): 1091-1094. http://www.scopus.com/inward/record.url?eid=2-s2.0-0343307136&partnerID=MN8TOARS.
    10.1016/S0015-0282(97)81444-6
  319. Sousa, M.; Barros, A.; Mendoza, C.; Tesarik, J.. "Effects of protein kinase C activation and inhibition on sperm-, thimerosal-, and ryanodine-induced calcium responses of human oocytes". Molecular Human Reproduction 2 9 (1996): 699-708. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030224788&partnerID=MN8TOARS.
    10.1093/molehr/2.9.699
  320. Tesarik, J.; Sousa, M.. "Calcium oscillations in human oocytes: Mechanism of calcium oscillations in human oocytes: A two-store model". Molecular Human Reproduction 2 6 (1996): 383-386. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030154304&partnerID=MN8TOARS.
  321. Sousa, M.; Barros, A.; Tesarik, J.. "Developmental changes in calcium dynamics, Protein Kinase C distribution and Endoplasmic Reticulum organization in human preimplantation embryos". Molecular Human Reproduction 2 12 (1996): 967-977. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030325275&partnerID=MN8TOARS.
    10.1093/molehr/2.12.967
  322. Sousa, M.; Mendoza, C.; Barros, A.; Tesarik, J.. "Calcium responses of human oocytes after intracytoplasmic injection of leukocytes, spermatocytes and round spermatids". Molecular Human Reproduction 2 11 (1996): 853-857. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030275611&partnerID=MN8TOARS.
    10.1093/molehr/2.11.853
  323. Sousa, M.; Barros, A.; Tesarik, J.. "The role of ryanodine-sensitive Ca2+ stores in the Ca2+ oscillation machine of human oocytes". Molecular Human Reproduction 2 4 (1996): 265-272. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030112743&partnerID=MN8TOARS.
  324. Tesarik, J.; Sousa, M.; Mendoza, C.. "Sperm-induced calcium oscillations of human oocytes show distinct features in oocyte center and periphery". Molecular Reproduction and Development 41 2 (1995): 259-263. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029055268&partnerID=MN8TOARS.
    10.1002/mrd.1080410217
  325. Tesarik, J.; Sousa, M.. "More than 90% fertilization rates after intracytoplasmic sperm injection and artificial induction of oocyte activation with calcium ionophore". Fertility and Sterility 63 2 (1995): 343-349. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028798290&partnerID=MN8TOARS.
  326. Tesarik, J.; Sousa, M.. "Key elements of a highly efficient intracytoplasmic sperm injection technique: Ca2+fluxes and oocyte cytoplasmic dislocation". Fertility and Sterility 64 4 (1995): 770-776. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029153881&partnerID=MN8TOARS.
    10.1016/S0015-0282(16)57853-4
  327. Tesarik, J.; Sousa, M.; Testart, J.. "Human oocyte activation after intracytoplasmic sperm injection". Human Reproduction 9 3 (1994): 511-518. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028267269&partnerID=MN8TOARS.
    10.1093/oxfordjournals.humrep.a138537
  328. Sousa, M.; Oliveira, E.. "An ultrastructural study of Crassostrea angulata (Mollusca, Bivalvia) spermatogenesis". Marine Biology 120 4 (1994): 545-551. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001192430&partnerID=MN8TOARS.
    10.1007/BF00350074
  329. Tesarik, J.; Sousa, M.. "Comparison of Ca2+ responses in human oocytes fertilized by subzonal insemination and by intracytoplasmic sperm injection". Fertility and Sterility 62 6 I (1994): 1197-1204. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028173178&partnerID=MN8TOARS.
  330. Tesarik, J.; Sousa, M.; Testart, J.. "Erratum: Human oocyte activation after intracytoplasmic sperm injection (Human Reproduction (1994) 9 (511-518))". Human Reproduction 9 5 (1994): http://www.scopus.com/inward/record.url?eid=2-s2.0-0028304846&partnerID=MN8TOARS.
  331. Sousa, M.; Tesarik, J.. "Fertiliza1tion and early embryology: Ultrastructural analysis of fertilization failure after intracytoplasmic sperm injection". Human Reproduction 9 12 (1994): 2374-2380. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028566995&partnerID=MN8TOARS.
    10.1093/oxfordjournals.humrep.a138455
  332. Sousa, M.; Oliveira, E.. "Ultrastructural and cytochemical study of spermatogenesis in Donax trunculus (Mollusca, Bivalvia)". J.SUBMICROSC.CYTOL.PATHOL. 26 3 (1994): 305-311. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028150340&partnerID=MN8TOARS.
  333. Sousa, M.; Oliveira, E.. "An ultrastructural study of spermatogenesis in Helcion pellucidus (Gastropoda, Prosobranchia)". International journal of invertebrate reproduction and development 26 2 (1994): 119-126. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028039464&partnerID=MN8TOARS.
  334. Sousa, M.; Carvalheiro, J.. "A cytochemical study of the nucleolus and nucleolus-related structures during human spermatogenesis". Anatomy and Embryology 190 5 (1994): 479-487. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028072981&partnerID=MN8TOARS.
    10.1007/BF00235495
  335. Sousa M; Pinto R; Moradas-Ferreira P; Azevedo C. "Histochemical Studies of Jelly Coat of Marthasterias glacialis (Echinodermata, Asteroidea) Oocytes.". The Biological bulletin (1993): https://doi.org/10.2307/1542001.
    10.2307/1542001
  336. Sousa M; C. Azevedo. "Ultrastructural Histochemistry of Marthasterias glacialis (Echinodermata, Asteroidea) Gametes Before and After Fertilization.". The Biological bulletin (1993): https://doi.org/10.2307/1542002.
    10.2307/1542002
  337. Fernandes, P.A.; Moradas-Ferreira, P.; Sousa, M.. "Flocculation of Kluyveromyces marxianus is induced by a temperature upshift". Yeast 9 8 (1993): 859-866. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027244592&partnerID=MN8TOARS.
    10.1002/yea.320090806
  338. Sousa, M.; Moradas-Ferreira, P.; Azevedo, C.. "Presence of a trypsin-like protease in starfish sperm acrosome". Journal of Experimental Zoology 261 3 (1992): 349-354. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026831439&partnerID=MN8TOARS.
    10.1002/jez.1402610314
  339. Sousa, M.; Azevedo, C.. "Alkaline phosphatase and peroxidase activities in starfish cortical vesicles befor and after cortical reaction". Journal of Submicroscopic Cytology and Pathology 22 1 (1990): 97-102. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025266083&partnerID=MN8TOARS.
  340. Sousa, M.; Azevedo, C.. "Silver staining of spermatogenesis in the starfish marthasterias glacialis". Invertebrate Reproduction and Development 15 2 (1989): 105-108. http://www.scopus.com/inward/record.url?eid=2-s2.0-84948639322&partnerID=MN8TOARS.
    10.1080/07924259.1989.9672030
  341. Sousa, M.; Azevedo, C.. "Ultrastructural Localization of Calcium in the Acrosome and Jelly Coat of Starfish Gametes: Asteroidea/sperm acrosome/oocyte jelly/calcium". Development, Growth & Differentiation 31 3 (1989): 227-232. http://www.scopus.com/inward/record.url?eid=2-s2.0-84985801097&partnerID=MN8TOARS.
    10.1111/j.1440-169X.1989.00227.x
  342. Sousa, M.; Azevedo, C.. "Starfish sperm-oocyte jelly binding triggers functional changes in cortical granules - A study using acid phosphatase and ruthenium red ultrastructural histochemistry". Histochemistry 90 5 (1989): 353-357. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024543517&partnerID=MN8TOARS.
    10.1007/BF00508312
  343. Sousa, M.; Corral, L.; Azevedo, C.. "Ultrastuctural and cytochemical study of spermatogenesis in Scrobicularia plana (Mollusca, Bivalvia)". Gamete Research 24 4 (1989): 393-401. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024830751&partnerID=MN8TOARS.
    10.1002/mrd.1120240406
  344. Sousa, M.; Azevedo, C.. "Ultrastructure and silver-staining analysis of spermatogenesis in the sea urchin Paracentrotus lividus (Echinodermata, Echinoidea)". Journal of Morphology 195 2 (1988): 177-188. http://www.scopus.com/inward/record.url?eid=2-s2.0-84935999044&partnerID=MN8TOARS.
    10.1002/jmor.1051950205
  345. Sousa, M.; Azevedo, C.. "Comparative silver staining analysis on spermatozoa of various invertebrate species". International Journal of Invertebrate Reproduction and Development 13 1 (1988): 1-8. http://www.scopus.com/inward/record.url?eid=2-s2.0-0002515569&partnerID=MN8TOARS.
    10.1080/01688170.1988.10510337
  346. Sousa, M.; Azevedo, C.. "Fine structural study of the acrosome formation in the starfish Marthasterias glacialis (Echinodermata, Asteroidea)". Tissue and Cell 20 4 (1988): 621-628. http://www.scopus.com/inward/record.url?eid=2-s2.0-45549114141&partnerID=MN8TOARS.
    10.1016/0040-8166(88)90063-8
  347. Sousa, M.; Azevedo, C.. "Presence of ATPase and alkaline phosphatase activities in the starfish sperm acrosome". Cell Biology International Reports 12 12 (1988): 1049-1054. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024224138&partnerID=MN8TOARS.
    10.1016/0309-1651(88)90029-X
  348. Sousa, M.; Azevedo, C.. "Ultrastructural and histochemical observations of the cortical reaction in Marthasterias glacialis (Echinodermata, Asteroidea)". Journal of Submicroscopic Cytology 20 3 (1988): 629-633. http://www.scopus.com/inward/record.url?eid=2-s2.0-0023683908&partnerID=MN8TOARS.
  349. Sousa, M.; Moradas Ferreira, P.; Amorim, A.; Azevedo, C.. "Starfish acrosomal acid phosphatase: a cytochemical and biochemical study". Biology of the Cell 63 1 (1988): 101-104. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024192996&partnerID=MN8TOARS.
    10.1111/j.1768-322X.1988.tb00746.x
  350. Sousa M; Azevedo C. "Silver staining of the cortical reaction in oocytes of Marthasterias glacialis (Echinodermata, Asteroidea)". Canadian Journal of Zoology (1987):
    10.1139/z87-395
  351. Sousa M; Azevedo C. "Cytochemical study on the spermatozoon and at early fertilization in Marthasterias glacialis (Echinodermata, Asteroidea)". Biology of the Cell (1986):
  352. Sousa, M.; Azevedo, C.. "Acrosomal reaction and early events at fertilization in Marthasterias glacialis (Echinodermata: Asteroidea)". Gamete Research 11 2 (1985): 157-167. http://www.scopus.com/inward/record.url?eid=2-s2.0-0022000375&partnerID=MN8TOARS.
    10.1002/mrd.1120110206
  353. Sousa, M.; Azevedo, C.. "Fine structure of the spermatozoon of marthasterias glacialis (linnaeus) (echinodermata; asteroidea), with special reference to acrosomal morphology". International Journal of Invertebrate Reproduction 6 3 (1983): 171-180. http://www.scopus.com/inward/record.url?eid=2-s2.0-84910345586&partnerID=MN8TOARS.
    10.1080/01651269.1983.10510039
Artigo em revista (magazine)
  1. Sá, Rosália; Sousa, Mário; Barros, Alberto. "Alterações do espermograma: infertilidade masculina", Mãe me quer, 2017, https://www.maemequer.pt/engravidar/fertilidade/masculina/alteracoes-do-espermograma/.
Capítulo de livro
  1. Luís Rato; Pedro F. Oliveira; Mário Sousa; Branca M. Silva; Marco G. Alves. "Role of Reactive Oxygen Species in Diabetes-Induced Male Reproductive Dysfunction". 135-147. Elsevier, 2019.
    10.1016/b978-0-12-812501-4.00014-6
  2. Oliveira, P.F.; Sousa, M.; Monteiro, M.P.; Silva, B.; Alves, M.G.. "Pineal gland and melatonin biosynthesis". 465-471. 2018.
  3. Oliveira, P.F.; Sousa, M.; Monteiro, M.P.; Silva, B.; Alves, M.G.. "Pineal gland and regulatory function". 472-477. 2018.
  4. Rato, L.; Oliveira, P.F.; Sousa, M.; Silva, B.M.; Alves, M.G.. "Role of reactive oxygen species in diabetes-induced male reproductive dysfunction". 135-147. 2018.
  5. Sousa, Mário. "Basic aspects of spermatogenesis". Índia, 2017.
    DOI: 10.2174/97816810850051170101
  6. Meneses, MJ; Bernardino, RL; Sousa, Mário; Silva, BM; Sá, Rosália; Oliveira, Pedro F.; Alves, Marco G.. "Regulation of Testicular Glucose Metabolism in (Pre)Diabetes and its Implications to Male Reproductive Health". In Glucose Metabolism: Biochemistry, Regulation and Health Effects.. 2015.
  7. Sá, Rosália; Barros, Alberto; Sousa, Mário. "Reação acrossómica e fertilização". In Reprodução Humana Masculina: Princípios Fundamentais, editado por Fardilha, M; Vieira da Silva, J; Conde, M, 123-130. ARC Publishing, 2015.
    Publicado
  8. Sousa, Mário; Sá, Rosália; Alves, Cláudia; Barros, Alberto. "Gametogénese, Fecundação e Desenvolvimento Embrionário Pré-Implantação.". In Sexologia Médica, editado por Nuno Monteiro Pereira, 13-29. Portugal: Lidel ¿ edições técnicas, lda, 2014.
    Publicado
  9. Sá, Rosália; Barros, Alberto; Sousa, Mário. "Clonagem Reprodutiva". In Sexologia Médica, editado por Nuno Monteiro Pereira, 31-43. Porto, Portugal: Lidel - edições técnicas, lda., 2014.
    Publicado
  10. Sá, Rosália; Cremades, Nieves; Alves, Cláudia; Barros, Alberto; Sousa, Mário. "Haploidização". In Sexologia Médica, editado por Nuno Monteiro Pereira, 45-55. Portugal: Lidel - edições técnicas, lda., 2014.
    Publicado
  11. Sá, Rosália; Barros, Alberto; Sousa, Mário. "Células Estaminais". In Sexologia Médica, editado por Nuno Monteiro Pereira, 57-76. Portugal: Lidel - edições técnicas, lda., 2014.
    Publicado
  12. Sá, R.; Sousa, M.; Cremades, N.; Alves, C.; Silva, J.; Barros, A.. "In vitro maturation of spermatozoa". 425-452. 2007.
  13. Sá, Rosália; Sousa, Mário; Barros, Alberto. "Manual of the infertile couple.". In Biology in the Night, editado por Soares, Amadeu, 108-149. Edições Afrontamento, 2007.
    Publicado
  14. Sá, Rosália; Sousa, Mário; Cremades, Nieves; Alves, Cláudia; Silva, Joaquina; Barros, Alberto. "Developmental Biology, Reprodutive Cloning, Haploidization and Stem Cells.". In Sexual Medicine. Portuguese Society of Andrology, Lusophone University, 2006.
    Publicado
  15. Sá, Rosália; Sousa, Mário; Cremades, Nieves; Neves, Rui; Alves, Cláudia; Fernandes, Susana; Silva, Joaquina; Barros, Alberto. "In vitro maturation of sperm". In In Vitro Fertilization and Assisted Reproduction, editado por Gurgan, T; Demirol, A, 79-82. Bolonha, Itália: Medimond Publishing Group, 2005.
    Publicado
  16. Fernandes, Susana; Sousa, Mário; Barros, Alberto. "Os genes e o homem infértil.". In Tratado de Andrologia Clínica, 195-222. Portugal: Sociedade Portuguesa de Andrologia, 2000.
    Publicado
  17. M. Sousa; C. Azevedo. "Ultrastructure and AG Staining of Echinoderm Spermatogenesis". 115-129. Springer Berlin Heidelberg, 1990.
    10.1007/978-3-642-83965-8_9
Edição de livro
  1. Braga Falcão, Pedro; Sousa, Mário; Carvalho, José Carlos Silva. Bíblia: Os Quatro Evangelhos e os Salmos. Fundação Secretariado Nacional da Educação Cristã. 2019.
    Publicado
Livro
  1. Crisóstomo, L.; Alves, M.G.; Gorga, A.; Sousa, M.; Riera, M.F.; Galardo, M.N.; Meroni, S.B.; Oliveira, P.F.. Molecular mechanisms and signaling pathways involved in the nutritional support of spermatogenesis by sertoli cells. 2018.
    10.1007/978-1-4939-7698-0_11
  2. Oliveira, J.; Pereira, R.; Santos, R.; Sousa, M.. Evaluating runs of homozygosity in exome sequencing data - Utility in disease inheritance model selection and variant filtering. 2018.
    10.1007/978-3-319-94806-5_15
  3. Sá, R.; Sousa, M.. Androgens and the ovary. 2014.
  4. Lima, P.; Monteiro, S.M.; Sousa, M.; Machado, J.. A histological study of oogenesis in the freshwater mussel Anodonta cygnea (Linnaeus, 1758) in Mira lagoon, Portugal. 2012.
    10.4002/040.055.0206
  5. Marques, C.J.; Sousa, M.. Imprinted gene anomalies in sperm. 2010.
    10.1017/9781139169349.005
  6. Barros, A.; Sousa, M.. Spermatid injection: Current status. 2008.
    10.1017/CBO9780511547287.056
  7. Fardilha, M.; Vieira, S.I.; Barros, A.; Sousa, M.; Da Cruz E Silva, O.A.B.; Da Cruz E Silva, E.F.. Differential distribution of Alzheimer's amyloid precursor protein family variants in human sperm. 2007.
    10.1196/annals.1397.086
Poster em conferência
  1. Lobo de Almeida, Ana; Gonçalves, Ana; Barros, Alberto; Sousa, Mário; Sá, Rosália. "Analysis of human sperm acrosome reaction observing the secrets of quick techniques". Trabalho apresentado em UMIB Summit 2023, 2023.
  2. Lobo de Almeida, Ana; Gonçalves, Ana; Barros, Alberto; Sousa, Mário; Sá, Rosália. "Oncofertilidade: O impacto da bleomicina na capacidade reprodutiva de jovens adultos". Trabalho apresentado em 5º Encontro Nacional de Jovens Investigadores em Oncologia, 2023.
  3. Lobo de Almeida, Ana; Gonçalves, Ana; Barros, Alberto; Sousa, Mário; Sá, Rosália. "Bleomicina uma sirga contra o DNA dos espermatozoides humanos". Trabalho apresentado em XXXVIII Jornadas de Estudos da Reprodução, Sociedade Portuguesa de Medicina da Reprodução, 2023.
  4. Lobo de Almeida, Ana; Gonçalves, Ana; Barros, Alberto; Sousa, Mário; Sá, Rosália. "Bleomycin's effects on human sperm motility and vitality in vitro". Trabalho apresentado em 16ª edição do IJUP ¿ Encontro de Investigação Jovem da Universidade do Porto, 2023.
  5. Pereira, Sara; Bernardino, Raquel; Martins, Ana D; Moreira, Bruno P.; Barros, Alberto; Sousa, Mário; Paiva, Sandra; Oliveira, Pedro F.; Alves, Marco G.. "Identification of obesity-related genes in Sertoli cells and human spermatozoa.". Trabalho apresentado em XX Congreso de la Sociedad Española de Histología e Ingeniería Tisular, 2019.
    10.14670/HH-sehit19
  6. Moreira, Bruno; Martins, Ana; Monteiro, Mariana P; Sousa, Mário; Oliveira, Pedro F; Alves, Marco G. "Leptin modulates biogenesis and mitochondrial function in Sertoli cells ¿ new mechanisms with implications in male reproductive potential.". Trabalho apresentado em I NoTeS Congress, 2019.
  7. Sara Pereira; Bernardino, Raquel; Martins, Ana D; Moreira, Bruno P; Barros, Alberto; Sousa, Mário; Paiva, Sandra; Oliveira, Pedro F; Alves, Marco G. "Expression of Obesity-related genes is altered by Leptin-Ghrelin stimuli, in Human Sertoli Cells". Trabalho apresentado em VII AEIBAS Biomedical Congress, 2019.
  8. Martins, Ana; Monteiro, Mariana P; Silva, Branca M; Barros, Alberto; Sousa, Mário; Carvalho, Rui A; Oliveira, Pedro F; Alves, Marco G. "Effects of GLP-1 in the glucose metabolism of human Sertoli cells". Trabalho apresentado em I NoTeS Congress,, 2018.
  9. Martins, Ana; Monteiro, Mariana P; Sousa, Mário; Oliveira, Pedro F; Alves, Marco G. "Leptin, ghrelin and GLP-1 regulate the metabolic profile of human Sertoli cells". Trabalho apresentado em Primeiro Encontro do Doutoramento em Ciências Biomédicas do ICBAS, 2018.
  10. Martins, Ana; Monteiro, Mariana P; Silva, Branca M; Sousa, Mário; Carvalho, Rui A; Oliveira, Pedro F; Alves, Marco G. "Control of energy balance by glucagon like peptide-1 may affect the nutritional support of spermatogenesis.". Trabalho apresentado em 42nd Congress of The Federation of the European Biochemical Societies, 2017.
  11. Crisóstomo, Luís; Jarak, Ivana; Carvalho, Rui; Sousa, Mário; Barros, Alberto; Alves, Marco G.; Oliveira, Pedro F.. "Glycerol metabolism in testicular cells ¿ a mitochondrial tale that may control male reproductive potential". 2017.
  12. Martins, Ana; Moreira, Bruno; Barros, Alberto; Sousa, Mário; Monteiro, Mariana P; Oliveira, Pedro F; Alves, Marco G. "Ghrelin, leptin and glp-1 as modulators of the nutritional support of spermatogenesis.". Trabalho apresentado em 24th European Congress on Obesity (ECO2017), 2017.
  13. Bernardino, Raquel L.; Dias, Tania Rodrigues; Oliveira, Elsa; Barros, Alberto; Sousa, Mário; Alves, Marco Gouveia; Oliveira, Pedro Fontes. "Morphological and metabolic alterations in human Sertoli cells induced by inhibition of carbonic anhydrase". Trabalho apresentado em 42nd Federation of European Biochemical Societies Congress, 2017.
  14. Bernardino, Raquel L.; Dias, Tânia R.; Oliveira, Elsa; Barros, Alberto; Sousa, Mário; Alves, Marco G.; Oliveira, Pedro F.. "Inhibition of carbonic anhydrase induces alterations in morphology and metabolism of human Sertoli cells". Trabalho apresentado em 6th i3S Annual Meeting, 2017.
  15. Martins, Ana; Alves, Marco G; Jarak, Ivana; Barros, Alberto; Silva, Joaquina; Sousa, Mário; Oliveira, Pedro F. "Is intratesticular lactate and creatine content a reliable biomarker of testicular dysfunction in men with Klinefelter syndrome?". Trabalho apresentado em 2nd International Workshop on Klinefelter Syndrome 2016, 2016.
  16. Bernardino, Raquel L.; Alves, Marco Gouveia; Silva, Joaquina; Barros, Alberto; Ferraz, luis; Sousa, Mário; Sá, Rosália; Oliveira, Pedro Fontes. "Expression of estrogen receptors in testicular tissue of individuals with Klinefelter syndrome". Trabalho apresentado em 2nd International Workshop on Klinefelter Syndrome, 2016.
  17. Almeida, Susana P; Martins, Ana; Jarak, Ivana; Barros, Alberto; Sousa, Mário; Oliveira, Pedro F; Alves, Marco G. "Testicular metabolic biomarkers in men with Klinefelter syndrome". Trabalho apresentado em UMIB Summit 2015, 2015.
  18. Martins, Ana; Sá, Rosália; Monteiro, Mariana P; Barros, Alberto; Silva, Joaquina; Sousa, Mário; Silva, Branca M; Oliveira, Pedro F.; Alves, Marco G. "Ghrelin modulates human Sertoli cells metabolism: relevance for male fertility.". Trabalho apresentado em 40th FEBS Congress, The Biochemical Basis of Life 2015, 2015.
  19. Dias, Tânia R; Alves, Marco G; Bernardino, Raquel L.; Martins, Ana; Moreira, Ana C; Silva, Joaquina; Barros, Alberto; et al. "Caffeine induced alterations in human Sertoli cells metabolism and oxidative profile". Trabalho apresentado em 40th FEBS Congress, The Biochemical Basis of Life 2015, 2015.
  20. Meneses, Maria J; Bernardino, Raquel L.; Sá, Rosália; Silva, Joaquina; Barros, Alberto; Sousa, Mário; Silva, Branca Maria; Oliveira, Pedro Fontes; Alves, Marco Gouveia. "Pioglitazone alone alters the metabolic support of spermatogenesis but not in combination with metformin: in vitro evidence". Trabalho apresentado em III AEICBAS Biomedical Congress, 2015.
  21. Jesus, Tito T.; Bernardino, Raquel L.; Martins, Ana D; Sá, Rosália; Sousa, Mário; Alves, Marco G.; Oliveira, Pedro Fontes. "Aquaporins-4 and -9 are expressed in rat Sertoli cells and interact with the CFTR". Trabalho apresentado em IV Encontro Nacional de Pós-graduação em Ciências Biológicas, 2015.
  22. Bernardino, Raquel L.; Alves, Marco Gouveia; Silva, Joaquina; Barros, Alberto; Ferraz, luis; Sousa, Mário; Sá, Rosália; et al. "Estrogen receptors alpha (ER-a), beta (ER-ß) and G protein-coupled receptor 30 (GPR30) in the testicular tissue of individuals with Klinefelter syndrome". Trabalho apresentado em UMIB Summit 2015, 2015.
    10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a4751
  23. Alves, Marco G; Jesus, Tito T; Bernardino, Raquel L.; Martins, Ana; Sá, Rosália; Sousa, Mário; Oliveira, Pedro F.. "Water transport in seminiferous tubule: Aquaporins identification in Sertoli cells". Trabalho apresentado em XXXVII IUPS Congress 2013, 2013.
  24. Bernardino, Raquel L.; Martins, Ana; Jesus, Tito T; Sá, Rosália; Sousa, Mário; Socorro, Sílvia; Alves, Marco G; Oliveira, Pedro F.. "Regulation by estrogens of bicarbonate secretion mechanisms in Sertoli cells". Trabalho apresentado em XXVII IUPS Congress 2013, 2013.
Pré-impressão
  1. Pereira RR; Carvalho V; Dias C; Barbosa T; Oliveira J; Alves Â; Oliveira E; Sá R; Sousa M. "Characterization of a DRC1 null variant associated to primary ciliary dyskinesia and female infertility". 2022. http://europepmc.org/abstract/PPR/PPR542794.
    10.21203/rs.3.rs-1971584/v1
Resumo em conferência
  1. Carrageta, David F; Oliveira, Pedro F.; Barros, Alberto; Sousa, Mário; Monteiro, Mariana P.; Alves, Marco G.. "Hyperglycemia induces capacitation and ROS overproduction on human sperm". Trabalho apresentado em European and International Congress on Obesity 2020, 2020.
    Publicado
  2. Carrageta, David F; Oliveira, Pedro F.; Barros, Alberto; Sousa, Mário; Monteiro, Mariana P.; Alves, Marco G.. "Hiperglicemia promove a capacitação e hiperativação mitocondrial em espermatozoides humanos.". Trabalho apresentado em 16º Congresso Português de Diabetes, 2020.
  3. Pereira, Sara C.; Martins, Ana D; Moreira, Bruno P.; Bernardino, Raquel L.; Barros, Alberto; Sousa, Mário; Oliveira, Pedro F.; Alves, Marco G.. "Assessment of the expression of obesity-related genes in human sertoli cells and spermatozoa". Trabalho apresentado em SEHIT 2019, 2019.
    Publicado • 10.14670/HH-sehit19
  4. Pereira, Sara C.; Martins, Ana D; Moreira, Bruno P.; Bernardino, Raquel L.; Barros, Alberto; Sousa, Mário; Paiva, Sandra; Oliveira, Pedro F.; Alves, Marco G.. "Ghrelin and Leptin stimuli alter the expression of Obesity-related genes in Sertoli Cells". Trabalho apresentado em 12th Meeting of Young Researchers of University of Porto, Porto, 2019.
    Publicado
  5. Almeida, SP; Jarak, I.; Carvalho, R. A.; Barros, A.; Sousa, Mário; Alves, Marco G.; Oliveira, PF. "The impact of ageing on the metabolic profile of rat testicular tissue: from the onset to the senescence". Trabalho apresentado em FEBS Open Bio, 2018.
    10.1002/2211-5463.12453
  6. Monteiro, JP; Jesus, TT; Crisóstomo, L.; Sousa, Mário; Barros, A.; Oliveira, Pedro F.; Alves, Marco G.. "mTOR coordinates the response to available energy substrates in human Sertoli cells: A defining point for male fertility?". Trabalho apresentado em The FEBS Journal, 2017.
    10.1111/febs.14171
  7. Bernardino, Raquel L.; Dias, Tânia R.; Oliveira, Elsa; Barros, Alberto; Sousa, Mário; Alves, Marco G.; Oliveira, Pedro F.. "Morphological and metabolic alterations in human Sertoli cells induced by inhibition of carbonic anhydrase. DOI:10.1111/febs.14174". 2017.
    DOI:10.1111/febs.14174
  8. Meneses, M.J.; Bernardino, Raquel L.; Sá, Rosália; Silva, Joaquina; Barros, Alberto; Sousa, Mário; Silva, Branca M.; Oliveira, Pedro F.; Alves, Marco G.. "The antidiabetic drugs Pioglitazone and Metformin alter the metabolic cooperation in the testis". Trabalho apresentado em IV Encontro Nacional de Pós-Graduação em Ciências Biológicas, Aveiro, 2016.
    Publicado
  9. Bernardino, Raquel L.; Alves, Marco G.; Silva, Joaquina; Barros, Alberto; Ferraz, Luís; Sousa, Mário; Sá, Rosália; Oliveira, Pedro F.. "Men with Klinefelter syndrome present altered testicular expression of estrogen receptors. DOI: 10.1111/eci.12603". 2016.
    Publicado • DOI: 10.1111/eci.12603
  10. Rabaça, Ana; Gonçalves, A.; Ferreira, C.; Bernardino, Raquel L.; Barros, Alberto; Alves, Marco G.; Sousa, Mário; Oliveira, Pedro F.; Sá, Rosália. "Protective effects of n-acetylcysteine on human sperm DNA during short-term etoposide exposure. DOI:10.1097/MD.0000000000002371". 2016.
    DOI:10.1097/MD.0000000000002371
  11. Ferreira, C.; Gonçalves, A.; Rabaça, Ana; Bernardino, Raquel L.; Barros, Alberto; Oliveira, Pedro F.; Sousa, Mário; Alves, Marco G.; Sá, Rosália. "Metformin preserves human sperm DNA integrity during short-term incubation. DOI:10.1097/MD.0000000000002371". 2016.
    DOI:10.1097/MD.0000000000002371
  12. Meneses, Maria J; Bernardino, Raquel L.; Sá, Rosália; Silva, Joaquina; Barros, Alberto; Sousa, Mário; Silva, Branca M.; Oliveira, Pedro F.; Alves, Marco G.. "The antidiabetic drug pioglitazone alters human Sertoli cells metabolism by increasing its glycolytic efficiency. DOI: 10.1097/MD.0000000000002371". 2016.
    DOI: 10.1097/MD.0000000000002371
  13. Dias, Tania Rodrigues; Alves, Marco Gouveia; Bernardino, Raquel L.; Martins, Ana D; Moreira, Ana Carolina; Silva, Joaquina; Barros, Alberto; et al. "Caffeine-induced alterations in human Sertoli cells metabolism and oxidative profile. DOI: 10.1111/febs.13339.". Trabalho apresentado em FEBS, 2015.
    Publicado
  14. Cunha, M; Dória, Sofia; Lima, Vera; Pinho, MJ; Almeida, C; Oliveira, C; Gonçalves, A; Sousa, Mário; Barros, Alberto. "Prevalence,charaterization and distribution of sex chromosome abnormalities in 9,448 Portuguese infertile patients". Trabalho apresentado em ESHRE, 2007.
    Publicado
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2020 31 research projects (13 as PI). Author/co-author 584 publications (496 International + 88 National), with 10992 citations, h index=50, and i10 index=191. Author/co-author 860 communications (299 International and 561 National). Participation in the discovery of new pathologic variants in infertile and Primary ciliary dyskinesia patients (12, in gene databases). Supervision/co-supervision 8 post-doctorate, 32 PhD and 46 MSc students. Member in 107 Scientific Juries. Member of one international patent (WIPO PCT/IB2018/055715)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2019 - 2019 The genetics of Primary Ciliary Dyskinesia: from the flagellum to the cilium
Orientador
Bioengenharia (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Fatores preditivos de recém-nascido em ciclos de microinjeção intracitoplasmática com espermatozóides do ejaculado
Orientador
Medicina (Mestrado)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2017 - 2017 Is Genistein a Threat to Male Reproductive Potential?
Coorientador
Biologia Molecular e Celular (Mestrado)
Universidade de Aveiro, Portugal
2015 - 2015 Microdeleções no cromossoma Y em azoospermia não obstrutiva e oligozoospermia severa
Orientador
Biologia Molecular e Celular (Mestrado)
Universidade de Aveiro, Portugal
2014 - 2014 Mutation analysis of genes involved in sperm motility: A study on patients with total sperm immotility
Orientador
Biologia Celular e Molecular (Mestrado)
Universidade do Porto Faculdade de Ciências, Portugal
2013 - 2013 Estudo Epidemiológico da Infertilidade - Prevalência e Importância Médico-Legal
Orientador
Medicina Legal (Mestrado)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2001 - 2001 Função das Oscilações de Cálcio Induzidas pelo Sperm Factor no Desenvolvimento Embrionário e na Apoptose
Coorientador
Ciências Biomédicas (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2001 - 2001 Caracterização Bioquímica, Imunológica e Molecular de Diferentes Tipos Celulares do Epitélio Branquial de Tilápia, Oreochromis Niloticus, Após Exposição Aguda e Crónica ao Sulfato de Cobre
Coorientador
Ciências Biológicas (Doutoramento)
Universidade de Trás-os-Montes e Alto Douro, Portugal

Júri de grau académico

Tema
Tipo de participação
Nome do candidato (Tipo de grau)
Instituição / Organização
2019/11/14 Member of the Scientific Jury in Academic Proofs: 92 several (Doutoramento)

Tutoria

Tópico Nome do aluno
1993/01/01 - 2020/02/20 Postgraduate supervision/cosupervision: Master of Science: 39 Postgraduate supervision/cosupervision: Doctorat: 30 Postgraduate supervision/cosupervision: Posdoctorat: 8 Postgraduate supervision/cosupervision: Investigators: 1 several
Distinções

Prémio

2020 Author/co-author in 66 Prizes and Nominations