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Mário Carvalho graduated from a Bachelor in Biomedical Sciences, minor in Molecular Biomedicine, in 2012 at the University of Aveiro (highest percentile, 17/20) and moved to Coimbra to enrol on the Masters in Cellular and Molecular Biology, minor in Neurobiology. He developed a thesis entitled "Molecular Mechanism of the synaptic and cognitive effects of ghrelin", under the supervision of Prof. João Peça and Prof. Ana Luísa Carvalho, and graduated in 2014 with 19/20 (highest percentile) winning an academic scholarship attributed to students with the highest score during that year. This work sparked his interest in neurophysiology and the implementation and optimization of scientific techniques. Between 2015 and 2020, he conducted and concluded his PhD in the MIT-Portugal Program in Bioengineering, coordinated by NOVA University of Lisbon (Portugal) and Center for Neuroscience and Cell Biology (CNC) (Portugal), and supervised by Prof. João Peça, Prof. Ana Luísa Carvalho and Prof. Manuel Nunes da Ponte. He also had the opportunity to work under the supervision of Inbal Israeli at the Champalimaud Program for Neuroscience (Lisbon, Portugal), for 2 months, where he developed Matlab scripts for analysing two-photon calcium imaging data. At CNC he became partially responsible for the implementation and optimization of several major techniques: brain slice electrophysiology, animal behavior and surgical application of cannulas for intracerebral injection. He was trained in electrophysiology by Paulo Pinheiro at the CNC and won a FENS Grant in order to attend the ELECTRAIN course on in vitro electrophysiology (2016) at the European Neuroscience Institute (Göttingen, Germany). He became proficient in these techniques and trained several, Master, PhD student and Postdocs. This work culminated in the publication of a Nature Communications paper (2019) describing the role of Gprasp2 in autism-like behaviours that won the prestigious 2019 prize from Pfizer/Society of Medical Sciences of Lisbon. For his PhD thesis, titled "Peptides that signal hunger - a new role in memory and social behaviors", he explored how the brain is changed by social subordination and early life stress, two important contributing factors for psychiatric disorders, and the role of neuropeptide Y signalling in these processes. This work resulted in a publication as co-first author in Neuropsychopharmacology (2020). He also used animal behavior tests to show, for the first time, the role of constitutive ghrelin receptor activity on memory formation, leading to a publication as co-first author in Science Signaling (2021). He presented this work at several scientific meetings and conferences. Immediately after his PhD, he started working as a contracted researcher in Paulo Pinheiros lab (at CNC), an expert in electrophysiology and synapse biology, in order to expand his skills in in vitro electrophysiology. Here, attracted by the complexity of the synapse and by its dysfunction in neuropsychiatric disorders, he began studying the functions of alternative SNAP protein isoforms. In summary, his yet short career in science already resulted in the publication of 3 important papers in high-impact international journals, of which he is co-first author in two. All were published in Q1 or top 10% journals, have an average Impact Factor of >9.1 and, despite very recent, accumulated to date 13 citations (h-index = 2). He presented his work in several scientific meetings/conferences. He played an indispensable part in the implementation and optimization of highly used techniques that were not previously available at the CNC and only rarely available in Portugal. His work on these techniques became a cornerstone of the publications he co-authored and provided an important contribution to the majority of scientific projects in the labs where he worked. His highly collaborative attitude also contributed to the training of several students in electrophysiology techniques.
Identificação

Identificação pessoal

Nome completo
Mário Jorge da Silva Carvalho

Nomes de citação

  • Carvalho, MJ

Identificadores de autor

Ciência ID
4719-AA2B-2AF1
ORCID iD
0000-0001-5904-2385
Google Scholar ID
https://scholar.google.pt/citations?hl=en&user=vYoSHq8AAAAJ

Moradas

  • Centro de Neurociências e Biologia Celular, Rua Larga, 1º Piso da FMUC, Universidade de Coimbra, 3004-504, Coimbra, Coimbra, Portugal (Profissional)

Websites

  • http://www.cnbc.pt/research/department_group_show.asp?iddep=1107&idgrp=1113 (Profissional)
  • https://www.cibb.uc.pt/research/neuroscience-disease/synapse-biology/ (Profissional)

Domínios de atuação

  • Ciências Médicas e da Saúde - Medicina Básica - Neurociências
  • Ciências Naturais - Ciências Biológicas - Biologia das Ciências do Comportamento
  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências Naturais - Ciências Biológicas - Biologia Molecular

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2)
Formação
Grau Classificação
2015/01 - 2020/07
Concluído
Bioengineering (Doutoramento)
Especialização em Programa Doutoral em Bioengenharia (MIT) - Bioengenharia (MIT)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Peptides that signal hunger - a new role in memory and social behaviors" (TESE/DISSERTAÇÃO)
18/20
2014/09 - 2014/10
Concluído
DGAV Accreditation for the Performance of Animal Behaviour Procedures (Outros)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2012/09 - 2014/09
Concluído
Cellular and Molecular Biology (Mestrado integrado)
Especialização em Neurobiologia
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Molecular mechanisms of the synaptic and cognitive effects of ghrelin" (TESE/DISSERTAÇÃO)
19/20
2009/09 - 2012/08
Concluído
Biomedical Sciences (Licenciatura)
Especialização em Molecular Biomedicine
Universidade de Aveiro, Portugal
17/20
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2020/09 - 2021/09 Investigador Contratado (Investigação) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento
Empregador
2021/09 - 2021/12 Professor Auxiliar Convidado (Docente Universitário) Universidade de Coimbra Departamento de Ciências da Vida, Portugal
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
Projetos

Bolsa

Designação Financiadores
2020 - 2021/09 Alternate SNAPs in neurotransmitter release: from molecular function to neurocognitive dysfunction
PTDC/BIA-CEL/29451/2017
Investigador Pós-doutorado
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2019 - 2020/01 Desenvolvimento de ferramentas moleculares para a doença de Machado-Joseph: moduladores de conformações tóxicas em proteínas com poliglutaminas
POCI-01-0145-FEDER-031173
Bolseiro de Investigação
European Regional Development Fund

Fundação para a Ciência e a Tecnologia
2015 - 2018/12 Peptides that signal hunger – a new role in memory and social behaviors
PD/BD/105878/2014
Bolseiro de Doutoramento
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em revista
  1. Ribeiro, Luís F.; Catarino, Tatiana; Carvalho, Mário; Cortes, Luísa; Santos, Sandra D.; Opazo, Patricio O.; Ribeiro, Lyn Rosenbrier; et al. "Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation". Science Signaling 14 670 (2021): eabb1953. http://dx.doi.org/10.1126/scisignal.abb1953.
    Publicado • 10.1126/scisignal.abb1953
  2. Coelho, Patrícia; Lourenço, Ana Sofia; Ferreira, Íldete Luísa; Marinho, Daniela; Anjo, Sandra Isabel; Mas, Philippe J.; Carvalho, MJ; et al. "The postsynaptic scaffolding protein SAPAP3 controls mitochondrial dynamics and function". Nature Communications (2021):
    Submetido
  3. Franco, Lara O.; Carvalho, Mário J.; Costa, Jéssica; Ferreira, Pedro A.; Guedes, Joana R.; Sousa, Renato; Edfawy, Mohamed; et al. "Social subordination induced by early life adversity rewires inhibitory control of the prefrontal cortex via enhanced Npy1r signaling". Neuropsychopharmacology 45 9 (2020): 1438-1447. http://dx.doi.org/10.1038/s41386-020-0727-7.
    Publicado • 10.1038/s41386-020-0727-7
  4. Edfawy, Mohamed; Guedes, Joana R.; Pereira, Marta I.; Laranjo, Mariana; Carvalho, Mário J.; Gao, Xian; Ferreira, Pedro A.; et al. "Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice". Nature Communications 10 1 (2019): http://dx.doi.org/10.1038/s41467-019-09382-9.
    Publicado • 10.1038/s41467-019-09382-9
Poster em conferência
  1. Luís Ribeiro; Tatiana Catarino; Carvalho, Mário; Sandra Santos; Luísa Cortes; Patrício Opazo; Lyn Rosenbrier Ribeiro; et al. "Constitutive ghrelin receptor activity modulates AMPA receptor traffic and supports memory formation". Trabalho apresentado em BEBday, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 2019.
  2. Luís Ribeiro; Tatiana Catarino; Carvalho, Mário; Sandra Santos; Luísa Cortes; Patrício Opazo; Lyn Rosenbrier Ribeiro; et al. "Constitutive ghrelin receptor activity supports memory formation and modulates hippocampal AMPA receptor traffic". Trabalho apresentado em 1º Retiro de Neurociência e Doença CIBB, 2018.
  3. Carvalho, Mário; Luís Ribeiro; Tatiana Catarino; Sandra Santos; José Esteban; Paulo Pinheiro; João Miguel Peça Lima Novo Silvestre; Ana Luísa Monteiro de Carvalho. "Constitutive activity of the ghrelin receptor controls AMPARs synaptic insertion in the hippocampus". Trabalho apresentado em 7th International Society of Neurochemistry Special Neurochemistry Conference, 2016.
  4. Luís Ribeiro; Carvalho, Mário; Tatiana Catarino; Sandra Santos; Luísa Cortes; Daniel Choquet; José Esteban; João Miguel Peça Lima Novo Silvestre; Ana Luísa Monteiro de Carvalho. "Constitutive activity of the ghrelin receptor controls AMPARs synaptic insertion in the hippocampus". Trabalho apresentado em EMBO Workshop in Neural Control of Metabolism and Eating Behavior, 2016.
Tese / Dissertação
  1. Carvalho, MJ. "Peptides that signal hunger a new role in memory and social behaviors". Doutoramento, Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, 2020.
  2. Carvalho, MJ. "Molecular mechanisms of the synaptic and cognitive effects of ghrelin". Mestrado, Universidade de Coimbra Departamento de Ciências da Vida, 2014.
Atividades

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2017/09/04 - 2017/09/14 2017 Summer School in Computational Biology - In this workshop I developed and acquired skill in computational biology. I attended lectures with themes such as: introductory mathematics and physics for biologists, mathematical models of disease spread, modeling of metabolic and signaling networks and molecular dynamics simulations of lipid bilayers. The workshop culminated in the preparation and presentation of a mini-research project about modeling disease spread using ¿small-world¿ networks.
Oficina (workshop)
2017 Summer School in Computational Biology
Universidade de Coimbra Departamento de Física, Portugal
2016/09/20 - 2016/09/24 Bernstein Conference for Computational Neuroscience 2016, and Dendritic Processing Workshop - In this conference I attended several lectures presented by scientist working on the forefront of computational neuroscience. I also attended a workshop that focused on how can dendritic processing can be explained using computational models.
Oficina (workshop)
Bernstein Conference for Computational Neuroscience 2016, and Dendritic Processing Workshop
Humboldt-Universität zu Berlin, Alemanha
2016/05/09 - 2016/05/20 ELECTRAIN course on in vitro electrophysiology, European Neuroscience Institute - In this workshop I attended lecture with experts in the field of in vitro electrophysiology, in a wide range of themes including: In-vivo electrophysiology of identified neurons in Hirudo medicinalis and measurement of synaptic parameters in mouse hippocampal organotypic slices. This workshop culminated in the lab work and presentation of results on electrophysiology measurements of identified neurons in Hirudo medicinalis.
Oficina (workshop)
ELECTRAIN course on in vitro electrophysiology, European Neuroscience Institute
Georg-August-Universität Göttingen, Alemanha

Tutoria

Tópico Nome do aluno
2018 - 2021 Training in patch-clamp electrophysiology Ana Rafaela Oliveira
2016 - 2021 Training in patch-clamp electrophysiology Beatriz Rodrigues
2016 - 2021 Training in patch-clamp electrophysiology Mariana Laranjo
2016 - 2021 Training in patch-clamp electrophysiology Marta Pereira
2016 - 2018 Training in patch-clamp electrophysiology Joana Guedes
2016 - 2018 Training in patch-clamp electrophysiology Pasqualino De Luca
Distinções

Prémio

2019 2019 Prémio Pfizer/Sociedade das Ciências Médicas de Lisboa
Pfizer Portugal, Portugal

Sociedade das Ciências Médicas de Lisboa, Portugal
2018 Academic Scholarship for highest scores on the academic year 2013/2014
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2016 FENS Grant for the participation in ELECTRAIN course on in vitro electrophysiology
Federation of European Neuroscience Societies, Bélgica