Tomás Onofre de Almeida Frazão (3611-1C91-ED32)

Curriculum Vitae

Tomás Onofre de Almeida Frazão

I graduated in Biochemistry (17/20) in 2019 at Faculdade de Ciências e Tecnologia (Universidade NOVA de Lisboa) - FCT NOVA. Beetween January and June of 2019 I did an intership at the Metalloproteins and Bioenergetics Unit - ITQB NOVA, in the scope of the final project of my degree, where I developled work on the O2, NO and H2O2 detoxification mechanisms of a flavodiiron protein from Firmicutes bacterium CAG:103. In the second half of 2019 I started a Master in Biochemistry for Health at ITQB NOVA (Instituto de Tecnologia Química e Biológica; Universidade Nova de Lisboa). In the second year of the Master, I joined the Microbial and Enzyme Technology (MET Lab) group at ITQB NOVA, where I was under the supervision of Patrícia Borges and Ligia Martins. The goals of the Master were the determination of the first crystal structure of a bacterial Pyranose 2-Oxidase (in collaboration with the Structural Biology Lab at ITQB), as well as the study of its biophysical properties and proposal of new variants by rational and semi-rational design. In 2022 (January) I successfully defended my Master's thesis entitled "Insight into the biophysical properties of AsP2Ox, a candidate enzyme for health diagnosis biosensors" (18/20). From December 2021 until August 2022, I did a 9 months secondment at Zymvol Biomodeling (Barcelona, Spain), as a project fellow of MET lab, in the frame of the B-LigZymes RISE European Commission Project, where I pursued knowledge on molecular modeling, as well as its application in enzymatic engineering. Docking and molecular dynamics are some of the tools learned. In October 2022 I completed a course in Python for Data Science, certified by UCSanDiegoX. I'm currently a second year PhD student at Met Lab (in colaboration with Zymvol Biomodeling), enroled in the ITQB-NOVA Molecular Biosciences (MolBioS) doctoral program. My PhD project can be defined as an iterative computational and laboratory engineering of bacterial oxidases towards biotechnological applications. I attended some international conferences and workshops where I was able to do poster and oral presentations, mainly abroad. My first arcticle publication, and as first author, was published on Nature Communication on 2023.

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Identificação

Identificação pessoal

Nome completo: Tomás Onofre de Almeida Frazão
Data de nascimento: 1998/03/30
Género: Masculino

Nomes de citação

Frazão, Tomás

Identificadores de autor

Ciência ID: 3611-1C91-ED32
ORCID iD: 0000-0001-5522-4085

Endereços de correio eletrónico

t.frazao@itqb.unl.pt (Profissional)

Idiomas

Idioma	Conversação	Leitura	Escrita	Compreensão	Peer-review
Português (Idioma materno)
Inglês	Utilizador independente (B2)	Utilizador independente (B2)	Utilizador independente (B2)	Utilizador independente (B2)	Utilizador independente (B2)

Formação

	Grau	Classificação
2023/01/01 - 2027/01/01 Em curso	Biociências Moleculares (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2022/10/11 Concluído	DSE200x: Python for Data Science (Outros) University of California San Diego, Estados Unidos
2019/09/16 - 2022/01/24 Concluído	Bioquímica para a Saúde (Mestrado) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal "Insight into the biophysical properties of AsP2Ox, a candidate enzyme for health diagnosis biosensors" (TESE/DISSERTAÇÃO)	18
2016/09 - 2019/06 Concluído	Bioquímica (Licenciatura) Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal	17

Percurso profissional

Ciência

	Categoria Profissional Instituição de acolhimento	Empregador
2020/09/14 - Atual	Investigador (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/12/06 - 2022/08/06	Estagiário de Investigação (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	ZYMVOL BIOMODELING SL, Espanha
2019/01 - 2019/06	Estagiário de Investigação (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Projetos

Bolsa

	Designação	Financiadores
2023/01/01 - 2026/12/31	Iterative Computational and Laboratory Engineering of Bacterial Carbohydrate Oxidases Towards Biotechnological Applications 2022.13872.BDANA Bolseiro de Doutoramento Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Em curso
2021/12/06 - 2024/07/31	B-LigZymes: Bacterial Enzymes and Bioprocesses for Lignin Valorisation. H2020-MSCA-RISE 2018 824017 Bolseiro de Investigação	Concluído

Produções

Publicações

Artigo em revista	Frazão, Tomás; Taborda, André; Rodrigues, Miguel V.; Fernández-Luengo, Xavier; Sancho, Ferran; Lucas, Maria Fátima; Frazão, Carlos; et al. Autor correspondente: Martins, Lígia O.. "Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-42000-3. Publicado • 10.1038/s41467-023-42000-3
Poster em conferência	Frazão, Tomás; Taborda, André; Lopes, Tiago; Sancho, Ferran; Borges, Patricia T.; Martins, Lígia. "Structural and Functional Insights of a New Bacterial Copper Radical Oxidase". Trabalho apresentado em 2nd bioexcel conference on advances in biomolecular simulations, 2024. Frazão, Tomás; Taborda, André; Lopes, Tiago; Sancho, Ferran; Borges, Patricia T.; Martins, Lígia. "Structural and Functional Insights of a New Bacterial Copper Radical Oxidase". Trabalho apresentado em ITQB NOVA PhD Meeting, 2024. Martins, Lígia; Taborda, André; Frazão, Tomás; Sancho, Ferran; Dias, Carolina; Costa, João; Lopes, Tiago; et al. "New Bacterial Carbohydrate Oxidases for Biotech Applications". Trabalho apresentado em OxiZymes 2024, 2024. Martins, Lígia; Taborda, André; Frazão, Tomás; Sancho, Ferran; Dias, Carolina; Costa, João; Jesus, Pedro; et al. "Engineering a Bacterial Galactose Oxidase for Green Biotech Applications". Trabalho apresentado em Meeting of the COST Action CA21162 COZYME Pan-European Network of Computational Redesign of Enzymes, 2024. Frazão, Tomás. "Insights into the structural determinants of substrate catalysis and specificity of bacterial C-glycoside oxidases". Trabalho apresentado em FEBS Computational Approaches to Understanding and Engineering Enzyme Catalysis, 2023. Martins, Lígia O.; Taborda, André; Frazão, Tomás. "Distinct structural and functional features of the new AA3 subfamily of glycoside 3-oxidases". Trabalho apresentado em Gordon Research Conference Carbohydrate-Active Enzymes for Glycan Conversions, 2023. Masgrau, Laura; Taborda, André; Frazão, Tomás. "Combined experimental and computational study for the characterization of Pseudarthrobacter siccitolerans". Trabalho apresentado em EuroCarb21, 2023. Taborda, André; Frazão, Tomás. "Unveiling the structural determinants for substrate recognition and catalysis of bacterial C-glycoside oxidases". Trabalho apresentado em BIOTRANS 2023, 2023. Taborda, André; Frazão, Tomás. "Structure-Function Relationships in Pseudarthrobacter siccitolerans Bacterial Glycoside 3-Oxidase". Trabalho apresentado em Novel Enzymes 2023, 2023. Frazão, Tomás. "Structural insights into a bacterial pyranose 2-oxidase from Pseudoarthrobacter siccitolerans, a candidate enzyme for health diagnosis biosensors". Trabalho apresentado em EFB2021 Virtual Conference, 2021.
Tese / Dissertação	"Insight into the biophysical properties of AsP2Ox, a candidate enzyme for health diagnosis biosensors". Mestrado, Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, 2022.

Atividades

Apresentação oral de trabalho

	Título da apresentação	Nome do evento Anfitrião (Local do evento)
2023/09/25	Short talk - Insights into the structural determinants of substrate catalysis and specificity of bacterial C-glycoside oxidases	FEBS Computational Approaches to Understanding and Engineering Enzyme Catalysis (Zagreb, Croácia)
2021/05/14	Flash poster presentation on the structural insights into the bacterial pyranose 2-oxidase from Pseudoarthrobacter siccitolerans, a candidate enzyme for health diagnosis biosensors	EFB2021 Virtual Conference European Federation of Biotechnology
2021/02/11	Update on the master thesis results: Insight into the biophysical properties of AsP2Ox, a candidate enzyme for health diagnosis biosensors	Jornadas de mestrados FCT-NOVA

Participação em evento

	Descrição da atividade Tipo de evento	Nome do evento Instituição / Organização
2025/01/23 - Atual	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	10th COZYME consortium webinar
2024/09/27 - Atual	Presenting the work done at the Microbial & Enzyme Technology Lab for people from all ages and with different backgrounds. Event organized between Oeiras Municipality and ITQB NOVA. Exposição	Noite dos Investigadores, Oeiras 2024
2024/09/25 - Atual	In-person Workshop to explore tools that aim to identify transferable skills and learn how to apply them in different professional contexts. Exploring key aspects of designing an appealing CV for academic or non-academic jobs. Oficina (workshop)	Identifiying Skills, CV & Cover Letter Wrting
2024/09/19 - Atual	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	9th COZYME consortium webinar
2024/07/25 - Atual	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	8th COZYME consortium webinar
2024/06/06 - Atual	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	7th COZYME consortium webinar
2024/02/01 - Atual	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	6th COZYME consortium webinar
2023/11/23 - Atual	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	5th COZYME consortium webinar
2023/09/14 - Atual	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs). Simpósio	4th COZYME consortium webinar
2024/11/26 - 2024/11/28	Hands-on 2.5-day workshop aimed for Ph.D. students and post-docs. Hybrid (on-site/online) event focusing on the use of BioExcel core codes: GROMACS, HADDOCK, PMX and BioBB; and will also include an Alphafold tutorial. Oficina (workshop)	BioExcel Coimbra 2024
2024/01/03 - 2024/01/05	The 2024 CCP4 Study Weekend included discussions and presentations by experts that highlighted the benefits that combination and cooperation of fully automated pipelines with manual interventions and fine-tuning can provide both for the users and for the quality and reliability of the final models Congresso	CCP4 Study Weekend 2024
2023/09/25 - 2023/09/29	FEBS course Computational Approaches to Understanding and Engineering Enzyme Catalysis Oficina (workshop)	FEBS Computational Approaches to Understanding and Engineering Enzyme Catalysis Federation of European Biochemical Societies, Reino Unido
2023/07/06 - 2023/07/06	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs). Simpósio	3rd COZYME consortium webinar
2023/05/04 - 2023/05/04	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	2nd COZYME webinar
2023/03/02 - 2023/03/02	Webinar of the COST Action COZYME (COmputationally assisted design of enZYMEs): tool for experienced and young researchers to share their work and knowledge with the rest of community (initial points of collaborations and STSMs) Simpósio	1st COZYME consortium webinar
2023/02 - 2023/02	Beyond the lab course (Zymvol, Barcelona): Computing and business skills for science student Oficina (workshop)	Beyond the lab ZYMVOL BIOMODELING SL, Espanha
2022/02/15 - 2022/02/15	Participation in the Re-Kick off Meeting of B-Ligzymes (RISE-H2020 project, coordinated by L. O. Martins, ITQB NOVA) dedicated results presentation, networking, and scientific discussions. Encontro	RISE-H2020 Bacterial Enzymes and Bioprocesses for Lignin Valorisation Re-Kick-OFF review meeting, 15 Feb 2022, online
2021/10/30 - 2021/10/30	Focused on vibrational spectroscopic approaches to elucidate structure-dynamics-function relationships in proteins and enzymes. Simpósio	TIMB3 Mini Symposium: The Role of Dynamics in Biocatalysis and the Methods to Study it Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/05/10 - 2021/05/14	Scientific conference organised by the European Federation of Biotechnology with plenary lectures and flash poster presentations. Conferência	EFB2021 Virtual Conference
2020/10/26 - 2020/10/27	Plenary lectures, short invited lectures and flash talks focusing on diverse aspects of biocatalysis Conferência	EFB Biocatalysts open day 2020