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My main area of expertise is invertebrate immunotoxicity induced by pollutants and other xenobiotics. During my M.S. and Ph.D. training I’ve investigated the effects of harmful algal toxins on the immune system of marine bivalves; and the effects of suppression of the antioxidant system of oysters on their immune status and susceptibility to infection. As a Postdoctoral Associate at Duke University, I started investigating if mitochondria are a major target of antimicrobial silver nanoparticles using mouse hepatocytes. Currently, I’m using the model organism Caenorhabditis elegans to understand how mitochondrial dysfunction induced by pollutant exposure early in life can result in life-lasting and intergenerational effects, with a particular interest in the effects on innate immunity. Throughout my academic career, I was constantly involved in teaching, beginning as a Teaching Assistant in Biochemistry and Cell Biology courses since my M.S. training, during which I had the opportunity to give several lectures. Now, as a Postdoctoral Associate, I taught a full Ecotoxicology course. Recently, translating science to the general public has become a new topic of interest of mine, therefore I’ve been also involved in different Science Communication and Outreach activities. Keywords: environmental toxicology; ecotoxicology; immunotoxicity; bivalve immunity; Caenorhabditis elegans; mitochondrial toxicity; invertebrate innate immunity; host-pathogen interactions; antioxidant system; pollution.
Identificação

Identificação pessoal

Nome completo
Danielle Ferraz Mello

Nomes de citação

  • Mello, Danielle F.

Identificadores de autor

Ciência ID
0918-75A4-5979
ORCID iD
0000-0003-0530-8277

Telefones

Telemóvel
  • (+1) 9195190230 (Pessoal)

Moradas

  • 2511 Croasdaile Farm Pkwy Apt 08, 27705, Durham, North Carolina, Estados Unidos (Pessoal)

Websites

  • https://www.daniellefmello.com/ (Profissional)

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C1)
Francês Utilizador independente (B1) Utilizador independente (B2) Utilizador elementar (A2) Utilizador independente (B2) Utilizador elementar (A2)
Espanhol; Castelhano Utilizador elementar (A1) Utilizador independente (B1) Utilizador elementar (A1) Utilizador independente (B1)
Formação
Grau Classificação
2012/03 - 2016/03
Concluído
Biochemistry (Doctor of Philosophy)
Universidade Federal de Santa Catarina, Brasil
"Detection of potential oyster pathogens in Santa Catarina and study of oyster immune and antioxidant-related defense mechanisms" (TESE/DISSERTAÇÃO)
2010/03 - 2012/02
Concluído
Biochemistry (Master Universitario)
Universidade Federal de Santa Catarina, Brasil
"Effects of harmful algae and phycotoxins in hemocytes of Pacific oysters Crassostrea gigas" (TESE/DISSERTAÇÃO)
2005/03 - 2009/12
Concluído
Biological Sciences (Bachelor)
Universidade Federal de Santa Catarina, Brasil
"Use of immunological markers in the clam Anomalocardia brasiliana for assessing the environmental quality of the Marine Extractive Reserve of Pirajubae, Florianopolis/SC." (TESE/DISSERTAÇÃO)
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2013/03 - 2014/02 Estagiário de Investigação (Investigação) Université de Montpellier, França
2009/08 - 2009/09 Estagiário de Investigação (Investigação) University of Plymouth, Reino Unido
2009/06 - 2009/07 Estagiário de Investigação (Investigação) Université de Montpellier, França

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento
Empregador
2019/01 - 2019/05 Leitor (Docente Universitário) Duke University, Estados Unidos

Cargos e Funções

Categoria Profissional
Instituição de acolhimento
Empregador
2016/04/04 - Atual Postdoctoral Associate Duke University, Estados Unidos
Projetos

Bolsa

Designação Financiadores
2012 - 2016/03 Detection of potential oyster pathogens in Santa Catarina and study of immune and antioxidant-related defense mechanisms in oysters
CAPES-DS
Bolseiro de Doutoramento
Universidade Federal de Santa Catarina, Brasil
2010 - 2012/02 Effects of harmful algae and phycotoxins in hemocytes of Pacific oysters Crassostrea gigas
CAPES-DS
Bolseiro de Mestrado
Universidade Federal de Santa Catarina, Brasil
2006 - 2010/02 Evaluation of immune parameters in marine bivalves exposed to environmental stressors
2006-2010/PIBIC/CNPq/UFSC
Bolseiro de Iniciação Científica
Universidade Federal de Santa Catarina, Brasil

Projeto

Designação Financiadores
2018 - Atual Effects of mitochondrial toxicants on innate immunity and pathogen resistance
5R01ES028218-03
Investigador Pós-doutorado
Duke University, Estados Unidos
2017 - Atual Does exposure to mitochondrial toxicants during germ cell development result in lifelong alterations in mitochondrial function mediated by epigenetic changes?
info:eu-repo/grantAgreement/NIH/NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES/1R01ES028218-01/US
Investigador Pós-doutorado
Duke University, Estados Unidos
National Institutes of Health
2017 - Atual Are mitochondria a major target of antimicrobial silver nanoparticles?
info:eu-repo/grantAgreement/NIH/NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES/5R21ES026743-02/US
Investigador Pós-doutorado
Duke University, Estados Unidos
National Institutes of Health
2016 - 2017/04 Genetic analysis of innate immunity using C. elegans
info:eu-repo/grantAgreement/NIH/NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES/5R01GM070977-09/US
Investigador Pós-doutorado
National Institutes of Health
Produções

Publicações

Artigo em revista
  1. Mello, Danielle Ferraz; Trevisan, Rafael; Danielli, Naissa Maria; Dafre, Alcir Luiz. "Vulnerability of glutathione-depleted Crassostrea gigas oysters to Vibrio species". Marine Environmental Research 154 (2020): 104870. http://dx.doi.org/10.1016/j.marenvres.2019.104870.
    10.1016/j.marenvres.2019.104870
  2. Mello, Danielle F.; Trevisan, Rafael; Rivera, Nelson; Geitner, Nicholas K.; Di Giulio, Richard T.; Wiesner, Mark R.; Hsu-Kim, Heileen; Meyer, Joel N.. "Caveats to the use of MTT, neutral red, Hoechst and Resazurin to measure silver nanoparticle cytotoxicity". Chemico-Biological Interactions 315 (2020): 108868. http://dx.doi.org/10.1016/j.cbi.2019.108868.
    10.1016/j.cbi.2019.108868
  3. Hartman, Jessica H.; Richie, Christopher T.; Gordon, Kacy L.; Mello, Danielle F.; Castillo, Priscila; Zhu, April; Wang, Yun; et al. "MANF deletion abrogates early larval Caenorhabditis elegans stress response to tunicamycin and Pseudomonas aeruginosa". European Journal of Cell Biology 98 5-8 (2019): http://dx.doi.org/10.1016/j.ejcb.2019.05.002.
    10.1016/j.ejcb.2019.05.002
  4. Maglioni, Silvia; Mello, Danielle F.; Schiavi, Alfonso; Meyer, Joel N.; Ventura, Natascia. "Mitochondrial bioenergetic changes during development as an indicator of C. elegans health-span". Aging 11 16 (2019): http://dx.doi.org/10.18632/aging.102208.
    10.18632/aging.102208
  5. Dreier, David A.; Mello, Danielle; Meyer, Joel; Martyniuk, Christopher J.. "Linking mitochondrial dysfunction to organismal and population health in context of environmental pollutants: Progress and considerations for mitochondrial adverse outcome pathways". Environmental Toxicology and Chemistry (2019): http://dx.doi.org/10.1002/etc.4453.
    10.1002/etc.4453
  6. Luz Cunha, Ana C.; Pontinha, Vitor de A.; de Castro, Maria Alcina M.; Sühnel, Simone; Medeiros, Sthefanie C.; Moura da Luz, Ângela M.; Harakava, Ricardo; et al. "Two epizootic Perkinsus spp. events in commercial oyster farms at Santa Catarina, Brazil". Journal of Fish Diseases 42 3 (2019): 455-463. http://dx.doi.org/10.1111/jfd.12958.
    10.1111/jfd.12958
  7. Hibshman, Jonathan D.; Leuthner, Tess C.; Shoben, Chelsea; Mello, Danielle F.; Sherwood, David R.; Meyer, Joel N.; Baugh, L. Ryan. "Nonselective autophagy reduces mitochondrial content during starvation in Caenorhabditis elegans". American Journal of Physiology-Cell Physiology 315 6 (2018): C781-C792. http://dx.doi.org/10.1152/ajpcell.00109.2018.
    10.1152/ajpcell.00109.2018
  8. Meyer, Joel N; Hartman, Jessica H; Mello, Danielle F. "Mitochondrial Toxicity". Toxicological Sciences 162 1 (2018): 15-23. http://dx.doi.org/10.1093/toxsci/kfy008.
    10.1093/toxsci/kfy008
  9. Mello, D F; Danielli, N M; Curbani, F; Pontinha, V A; Suhnel, S; Castro, M A M; Medeiros, S C; et al. "First evidence of viral and bacterial oyster pathogens in the Brazilian coast". Journal of Fish Diseases 41 3 (2017): 559-563. http://dx.doi.org/10.1111/jfd.12755.
    10.1111/jfd.12755
  10. Danielli, Naissa Maria; Trevisan, Rafael; Mello, Danielle Ferraz; Fischer, Kelvis; Deconto, Vanessa Schadeck; da Silva Acosta, Daiane; Bianchini, Adalto; Bainy, Afonso Celso Dias; Dafre, Alcir Luiz. "Upregulating Nrf2-dependent antioxidant defenses in Pacific oysters Crassostrea gigas: Investigating the Nrf2/Keap1 pathway in bivalves". Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 195 (2017): 16-26. http://dx.doi.org/10.1016/j.cbpc.2017.02.004.
    10.1016/j.cbpc.2017.02.004
  11. Nogueira, Lílian; Mello, Danielle Ferraz; Trevisan, Rafael; Garcia, Danielly; da Silva Acosta, Daiane; Dafre, Alcir Luiz; de Almeida, Eduardo Alves. "Hypoxia effects on oxidative stress and immunocompetence biomarkers in the mussel Perna perna (Mytilidae, Bivalvia)". Marine Environmental Research 126 (2017): 109-115. http://dx.doi.org/10.1016/j.marenvres.2017.02.009.
    10.1016/j.marenvres.2017.02.009
  12. Trevisan, Rafael; Flores-Nunes, Fabrício; Dolores, Euler S.; Mattos, Jacó J.; Piazza, Clei E.; Sasaki, Sílvio T.; Taniguchi, Satie; et al. "Thiol oxidation of hemolymph proteins in oysters Crassostrea brasiliana as markers of oxidative damage induced by urban sewage exposure". Environmental Toxicology and Chemistry 36 7 (2016): 1833-1845. http://dx.doi.org/10.1002/etc.3543.
    10.1002/etc.3543
  13. Trevisan, Rafael; Mello, Danielle F.; Delapedra, Gabriel; Silva, Danilo G.H.; Arl, Miriam; Danielli, Naissa M.; Metian, Marc; Almeida, Eduardo A.; Dafre, Alcir L.. "Gills as a glutathione-dependent metabolic barrier in Pacific oysters Crassostrea gigas : Absorption, metabolism and excretion of a model electrophile". Aquatic Toxicology 173 (2016): 105-119. http://dx.doi.org/10.1016/j.aquatox.2016.01.008.
    10.1016/j.aquatox.2016.01.008
  14. Uchoa, Mariana Figueiroa; de Souza, Luiz Felipe; dos Santos, Danubia Bonfanti; Peres, Tanara Vieira; Mello, Danielle Ferraz; Leal, Rodrigo Bainy; Farina, Marcelo; Dafre, Alcir Luiz. "Modulation of Brain Glutathione Reductase and Peroxiredoxin 2 by a-Tocopheryl Phosphate". Cellular and Molecular Neurobiology 36 6 (2016): 1015-1022. http://dx.doi.org/10.1007/s10571-015-0298-z.
    10.1007/s10571-015-0298-z
  15. Mello, Danielle Ferraz; Arl, Miriam; Trevisan, Rafael; Dafre, Alcir Luiz. "How important are glutathione and thiol reductases to oyster hemocyte function?". Fish & Shellfish Immunology 46 2 (2015): 566-572. http://dx.doi.org/10.1016/j.fsi.2015.07.017.
    10.1016/j.fsi.2015.07.017
  16. Trevisan, Rafael; Mello, Danielle Ferraz; Uliano-Silva, Marcela; Delapedra, Gabriel; Arl, Miriam; Dafre, Alcir Luiz. "The biological importance of glutathione peroxidase and peroxiredoxin backup systems in bivalves during peroxide exposure". Marine Environmental Research 101 (2014): 81-90. http://dx.doi.org/10.1016/j.marenvres.2014.09.004.
    10.1016/j.marenvres.2014.09.004
  17. Trevisan, Rafael; Delapedra, Gabriel; Mello, Danielle F.; Arl, Miriam; Schmidt, Éder C.; Meder, Fabian; Monopoli, Marco; et al. "Gills are an initial target of zinc oxide nanoparticles in oysters Crassostrea gigas, leading to mitochondrial disruption and oxidative stress". Aquatic Toxicology 153 (2014): 27-38. http://dx.doi.org/10.1016/j.aquatox.2014.03.018.
    10.1016/j.aquatox.2014.03.018
  18. Erik Simões; Renato Campos Vieira; Mathias Alberto Schramm; Danielle Ferraz Mello; Vitor De Almeida Pontinha; Patrícia Mirella da Silva; Margherita Anna Barracco. "Impact of harmful algal blooms (Dinophysis acuminata) on the immune system of oysters and mussels from Santa Catarina, Brazil". J. Mar. Biol. Ass. 95 04 (2014): 773-781. http://dx.doi.org/10.1017/s0025315414001702.
    10.1017/s0025315414001702
  19. Mello, Danielle Ferraz; Silva, Patricia Mirella da; Barracco, Margherita Anna; Soudant, Philippe; Hégaret, Hélène. "Effects of the dinoflagellate Alexandrium minutum and its toxin (saxitoxin) on the functional activity and gene expression of Crassostrea gigas hemocytes". Harmful Algae 26 (2013): 45-51. http://dx.doi.org/10.1016/j.hal.2013.03.003.
    10.1016/j.hal.2013.03.003
  20. Mello, Danielle F.; de Oliveira, Eliza S.; Vieira, Renato C.; Simoes, Erik; Trevisan, Rafael; Dafre, Alcir Luiz; Barracco, Margherita Anna. "Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2)". Marine Drugs 10 12 (2012): 583-597. http://dx.doi.org/10.3390/md10030583.
    10.3390/md10030583
  21. Trevisan, Rafael; Arl, Miriam; Sacchet, Cássia Lopes; Engel, Cristiano Severino; Danielli, Naissa Maria; Mello, Danielle Ferraz; Brocardo, Caroline; Maris, Angelica Francesca; Dafre, Alcir Luiz. "Antioxidant deficit in gills of Pacific oyster (Crassostrea gigas) exposed to chlorodinitrobenzene increases menadione toxicity". Aquatic Toxicology 108 (2012): 85-93. http://dx.doi.org/10.1016/j.aquatox.2011.09.023.
    10.1016/j.aquatox.2011.09.023
  22. Trevisan, Rafael; Ferraz Mello, Danielle; Fisher, Andrew S.; Schuwerack, Petra-Manuela; Dafre, Alcir Luiz; Moody, A. John. "Selenium in water enhances antioxidant defenses and protects against copper-induced DNA damage in the blue mussel Mytilus edulis". Aquatic Toxicology 101 1 (2011): 64-71. http://dx.doi.org/10.1016/j.aquatox.2010.09.003.
    10.1016/j.aquatox.2010.09.003
  23. Mello, Danielle Ferraz; Proença, Luis Antonio de Oliveira; Barracco, Margherita Anna. "Comparative Study of Various Immune Parameters in Three Bivalve Species during a Natural Bloom of Dinophysis acuminata in Santa Catarina Island, Brazil". Toxins 2 5 (2010): 1166-1178. http://dx.doi.org/10.3390/toxins2051166.
    10.3390/toxins2051166
Poster em conferência
  1. Mello, Danielle F.. "Linking mitochondrial dysfunction during development with pathogen resistance later in life.". Trabalho apresentado em 22nd International C. elegans Conference, 2019.
  2. Mello, Danielle F.. "Caenorhabditis elegans mitochondrial bioenergetics during development: advances using the Seahorse Extracellular Flux Analyzer". Trabalho apresentado em 22nd International C. elegans Conference, 2019.
  3. Mello, Danielle F.. "Pro-longevity suppression of different electron transport chain subunits mildly impact mitochondrial respiration and mtDNA copy number". Trabalho apresentado em C. elegans Stress, Pathogenesis, Aging, Metabolism and Small RNAs (SPAMS) Meeting, 2018.
  4. Mello, Danielle F.. "Overcoming experimental confounders when assessing silver nanoparticle toxicity". Trabalho apresentado em SETAC North America 38th Annual Meeting, 2017.
  5. Mello, Danielle F.. "Effects of glutathione depletion in a marine bivalve". Trabalho apresentado em SETAC Latin America 11th Biennial Meeting, 2015.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2020/01 Using invertebrate models to investigate chemical-induced immunotoxicity Duke Integrated Toxicology & Environmental Health Program Seminar Series
Duke University (Durham, Estados Unidos)
2019/11 Pollution and Toxicology Lecture for Environmental Science and Policy (ENV 102) course
Duke University (Durham, Estados Unidos)
2019 Effects of glutathione depletion in oyster immunocompetence and survival upon oxidative and bacterial challenges 20th International Symposium on Pollutant Responses in Marine Organisms
(Charleston, Estados Unidos)
2018/10 Nanotoxicology Lecture for Environmental Toxicology (ENV501) course
Duke University (Durham, Estados Unidos)
2018 Assessment of silver nanoparticle toxicity: overcoming experimental confounders and testing mitochondrial toxicity 13th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials (ICEENN 2018)
(Durham, Estados Unidos)
2018 Overcoming experimental confounders when assessing silver nanoparticle toxicity 7th Young Environmental Scientist's (YES) Meeting
(Madison, Estados Unidos)
2018 Rotenone-induced mitochondrial dysfunction alters pathogen resistance in Caenorhabditis elegans SETAC North America 39th Annual Meeting
(Sacramento, Estados Unidos)
2017/10 Nanotoxicology Lecture for Environmental Toxicology (ENV501) course
Duke University (Durham, Estados Unidos)
2016 How animals defend themselves against microorganisms 1st Science Day at North Carolina School of Science and Math (NCSSM)
(Durham, Estados Unidos)
2013 Antioxidant system and immunocompetence of pacific oyster hemocytes under a xenobiotic challenge 17th International Symposium on Pollutant Responses in Marine Organisms
(Faro, Portugal)
2012 Harmful algae and phycotoxins effects upon gene expression of defense related genes in oyster hemocytes XII Congresso Brasileiro de Ecotoxicologia
(Brasil)
2011 In vitro effects of Alexandrium minutum and saxitoxins upon hemocyte functions of Crassostrea gigas 16th International Symposium on Pollutant Responses in Marine Organisms
(Long Beach, Estados Unidos)
2009 Monitoramento da qualidade ambiental da Reserva Extrativista Marinha do Pirajubaé (REMAPI), Florianópolis/SC e da condição de saúde do Berbigão Anomalocardia brasiliana através da avaliação de parâmetros hemato-imunológicos 19° Seminário de Iniciação Científica da UFSC
(Brasil)
2009 Modulation of some hemato-immunological parameters in three bivalve species during a natural bloom of the dinoflagellate Dinophysis acuminata in Santa Catarina island, Brazil. 15th International Symposium on Pollutant Responses in Marine Organisms
(Bordeaux, França)

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2018/12/11 - 2018/12/12 The use of the nematode Caenorhabditis elegans and the zebrafish Danio rerio as model organisms in toxicology studies: from the environment to human health (2018/12/11)
Oficina (workshop) (Coorganizador)
Universidade Federal de Santa Catarina, Brasil
2018 - 2018 3rd Science Day at North Carolina School of Science and Math (NCSSM), Durham, NC. USA. (2018)
Outro (Coorganizador)
2017 - 2017 3rd Postdocumentaries: connecting Duke Postdocs to middle school students (2017)
Outro (Coorganizador)
2017 - 2017 2nd Science Day at North Carolina School of Science and Math (NCSSM), Durham, NC. USA. (2017)
Outro (Coorganizador)

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2018 - 2018 Science fair demo instructor about “Seeing what water pollution can do”. Goal: Learn how to use a microscope and visualize fish embryos (zebrafish) and worms (C. elegans) after toxic exposures.
Exposição
Lakewood’s Science Night
Lakewood Elementary School, Estados Unidos
2018 - 2018 Science fair workshop instructor about “worms with sunburns”. Goal: Teach the students about DNA damage by sunlight (UV light) and mention some repair mechanisms, and about how transgenic worms (C. elegans) can help in the research of harmful effects of environmental exposures.
Oficina (workshop)
Carolina Friends School Science Day
Carolina Friends School Middle School, Estados Unidos
2016 - 2016 Science day presentation about "How animals defend themselves against microorganisms".
Seminário
1st Science Day at North Carolina School of Science and Math (NCSSM)
North Carolina School of Science and Mathematics, Estados Unidos
2016 - 2016 Interviewed by middle school students about my scientific career (interview available on https://www.youtube.com/watch?v=rTu4eV6m_RA&list=FL9nibrZDGPJMApNgg7uzoyA)
Outro
Postdocumentaries
Duke University, Estados Unidos

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2019/01 - 2019/05 Ecotoxicology (ENVIRON610) Duke University, Estados Unidos
2010 - 2015 Biochemistry for Biological Sciences major students (Teaching assistant and lecturer) (Bachelor) Universidade Federal de Santa Catarina, Brasil

Membro de associação

Nome da associação Tipo de participação
2017/05 - 2019/02 Duke University Postdoc Association Outreach Chair

Outro júri / avaliação

Descrição da atividade Instituição / Organização
2018 - Atual Science fair judge - NC Science & Engineering Fair Region 3b North Carolina School of Science & Mathematics, Estados Unidos

Tutoria

Tópico Nome do aluno
2018/10 - Atual Shefali Bijwadia
2018/06 - Atual Christina Bergemann
2019/06 - 2019/08 Yang Wang
2019/06 - 2019/08 Alexis Caldwell
2018/10 - 2019/01 Sean Cho
2018/10 - 2018/12 Christine Bergamini
2016/04 - 2017/03 Caroline Bay
2014 - 2015 Fernando Curbani
2012 - 2013 Leandro Espindola
2010 - 2012 Eliza Simão de Oliveira
2010 - 2012 Renato Vieira
Distinções

Prémio

2008 Honors for Academic Performance
Universidade Federal de Santa Catarina, Brasil
2007 Honors for Academic Performance
Universidade Federal de Santa Catarina, Brasil
2006 Honors for Academic Performance
Universidade Federal de Santa Catarina, Brasil