Personal identification

Full name

José Gonçalo Deira Duarte de Campos Justino

Citation names

• Justino, Gonçalo C.
• Justino, Gonçalo

Author identifiers

Ciência ID

781D-3A77-5A3B

ORCID iD

0000-0003-4828-4738

Google Scholar ID

SeTbJlgAAAAJ&hl

Researcher Id

B-3190-2011

Scopus Author Id

6507263334

Email addresses

• goncalo.justino@tecnico.ulisboa.pt (Professional)

Telephones

Telephone

• 218417255 (Professional)

Addresses

• Av. Rovisco Pais, 1, 1049-001, Lisboa, Lisboa, Portugal (Professional)

Websites

• web.tecnico.ulisboa.pt/goncalo.justino (Professional)

Knowledge fields

• Natural sciences - Biological Sciences - Biochemistry
• Natural sciences - Biological Sciences - Biochemical Research Methods
• Medical and Health Sciences - Basic Medicine - Medicinal Chemistry

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Advanced (C1)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)
French	Elementary (A2)	Elementary (A2)	Beginner (A1)	Elementary (A2)	Beginner (A1)

	Degree	Classification
2007/05/27 Concluded	Bioquímica (Doutoramento) Major in Especialidade: Bioquímica Clínica e Farmacêutica Universidade de Lisboa, Portugal "Antioxidante com Estrutura Catecólica: Matabolismo e Relações Estrutura - Actividade" (THESIS/DISSERTATION)
1997/09 - 2002/07 Concluded	Bioquímica (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal	17

Science

	Category Host institution	Employer
2018 - Current	Researcher (Research)	Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
	Universidade de Lisboa Centro de Química Estrutural, Portugal
2009 - 2018	Postdoc (Research)	Universidade de Lisboa Instituto Superior Técnico, Portugal
2009/10/01 - 2010/01/31	Visiting Researcher (Research)	University of St Andrews School of Chemistry, United Kingdom
2007 - 2009	Postdoc (Research)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Teaching in Higher Education

	Category Host institution	Employer
2014 - Current	Invited Adjunct Teacher (Polytechnic Teacher)	Instituto Politécnico de Setúbal, Portugal

Positions / Appointments

	Category Host institution	Employer
2020/01/01 - 2021/12/31	Junior Coordinator of Research Group "Design, synthesis, and toxicology of bioactive molecules (BIOMOL)"	Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
	Universidade de Lisboa Centro de Química Estrutural, Portugal
2015 - 2015	Responsável pelas UCs Bioquímica Computacional e Laboratório de Bioinformática da proposta de criação da Licenciatura em Bioinformática	Instituto Politécnico de Setúbal Escola Superior de Tecnologia do Barreiro, Portugal
	Instituto Politécnico de Setúbal Escola Superior de Tecnologia do Barreiro, Portugal
2014 - 2014	Responsável pela UC Técnicas de Modelação Aplicadas à Biotecnologia na proposta de criação do Mestrado em Engenharia Biotecnológica e Química	Instituto Politécnico de Setúbal Escola Superior de Tecnologia do Barreiro, Portugal
	Instituto Politécnico de Setúbal Escola Superior de Tecnologia do Barreiro, Portugal

Grant

	Designation	Funders
2013 - 2013	Scalability of CP2K applied to metal-transporting proteins PRPA34 Principal investigator HPC-Europa2, Belgium	Partnership for Advanced Computing In Europe
2011/01 - 2011/12	Linking Regioselectivity and Toxicity of Nevirapine – a computational approach HPC-08Y9LZ7 Principal investigator HPC-Europa2, Belgium	HPC-Europa2
2009/09 - 2010/02	Computational study of Nevirapine reaction with proteins and DNA - an approach to hepatic toxicity HPC-04RZIA Principal investigator HPC-Europa2, Belgium	HPC-Europa

Contract

	Designation	Funders
2022/02/01 - Current	Probing MRSA mecA-dependent resistance mechanism by molecular dynamics simulations CPCA/A1/416303/2021 Principal investigator Universidade de Lisboa Centro de Química Estrutural, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2020 - Current	Centro de Química Estrutural UIDB/00100/2020 Researcher Universidade de Lisboa Centro de Química Estrutural, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2020 - Current	Centro de Química Estrutural UIDB/00100/2020 Researcher Universidade de Lisboa Centro de Química Estrutural, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2019 - Current	Centro de Química Estrutural UID/QUI/00100/2019 Researcher Universidade de Lisboa Centro de Química Estrutural, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/10/01 - 2021/09/30	Do Desenho de Fármacos à Toxicologia Química: modelação de ferramentas de previsão com Espectrometria de Massa PTDC/QUI-QAN/32242/2017 Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2013 - 2018	Centro de Química Estrutural PEst-OE/QUI/UI0100/2013 Researcher Universidade de Lisboa Centro de Química Estrutural, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/01 - 2013/12	Kinetic study of biorecalcitrant compounds degradation by pulse radiolysis PTDC/QUI-QUI/104229/2008 Researcher Universidade de Lisboa Instituto Superior Tecnico Campus Tecnológico e Nuclear, Portugal	Fundação para a Ciência e a Tecnologia
2011 - 2012	Centro de Química Estrutural PEst-OE/QUI&UI0100/2011 Researcher Universidade de Lisboa Centro de Química Estrutural, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008/01 - 2011/12	Reconstruction and systems analysis of the reaction networks of reactive oxygen, nitrogen and sulphur species in representative physiological systems PTDC/QUI/70523/2006 Researcher Universidade de Coimbra Departamento de Química, Portugal	Fundação para a Ciência e a Tecnologia

Publications

Book	Justino, Gonçalo C.. Tea - Chemistry and Pharmacology. InTech Open. 2020. Editor Justino, Gonçalo C.. Flavonoids - From Biosynthesis to Human Health. InTech. 2017. Published • 10.5772/65575
Book chapter	Gonçalo Justino. "Structural Analysis of Flavonoids and Related Compounds – a Review of Spectroscopic Applications". 2012. 10.5772/29152
Conference paper	Tiago AH Fonseca; M. Conceiçäo Oliveira; Rúben Araújo; Luís Bento; Cristiana Von Rekowski; Gonçalo C. Justino; Cecília R.C. Calado. "Comparison of Analytical Methods Of Serum Untargeted Metabolomics". 2023. 10.1109/ENBENG58165.2023.10175339
Edited book	Justino, Gonçalo C.. Tea - Chemistry and Pharmacology [Working Title]. IntechOpen. 2018. 10.5772/intechopen.73746
Journal article	Pedro M. G. Silva; Pedro F. Pinheiro; Sérgio P. Camões; Ana P. C. Ribeiro; Luísa M. D. R. S. Martins; Joana P. G. Miranda; Gonçalo C. Justino. "Exploring the Mechanisms behind the Anti-Tumoral Effects of Model C-Scorpionate Complexes". Molecules (2023): https://doi.org/10.3390/molecules28145451. 10.3390/molecules28145451 Tiago A. H. Fonseca; Cristiana P. Von Rekowski; Rúben Araújo; M. Conceição Oliveira; Gonçalo C. Justino; Luís Bento; Cecília R. C. Calado. "The Impact of the Serum Extraction Protocol on Metabolomic Profiling Using UPLC-MS/MS and FTIR Spectroscopy". ACS Omega (2023): https://doi.org/10.1021/acsomega.3c01370. 10.1021/acsomega.3c01370 Cátia F. Marques; Gonçalo C. Justino. "An Optimised MS-Based Versatile Untargeted Metabolomics Protocol". Separations (2023): https://doi.org/10.3390/separations10050314. 10.3390/separations10050314 Marques CF; Pinheiro PF; Justino GC. "Protocol to study in vitro drug metabolism and identify montelukast metabolites from purified enzymes and primary cell cultures by mass spectrometry.". STAR protocols (2023): https://europepmc.org/articles/PMC9929484. 10.1016/j.xpro.2023.102086 Ana Teresa Pinto; Ana Beatriz Machado; Hugo Osório; Marta Laranjeiro Pinto; Rui Vitorino; Gonçalo Justino; Cátia Santa; et al. "Macrophage Resistance to Ionizing Radiation Exposure Is Accompanied by Decreased Cathepsin D and Increased Transferrin Receptor 1 Expression". Cancers (2022): https://doi.org/10.3390/cancers15010270. 10.3390/cancers15010270 Marques CF; Marques MM; Justino GC. "The mechanisms underlying montelukast's neuropsychiatric effects - new insights from a combined metabolic and multiomics approach.". Life sciences (2022): https://doi.org/10.1016/j.lfs.2022.121056. 10.1016/j.lfs.2022.121056 Cátia F. Marques; Pedro Pinheiro; Justino GC. "Optimized protocol for obtaining and characterizing primary neuron-enriched cultures from embryonic chicken brains.". STAR protocols (2022): https://europepmc.org/articles/PMC9558106. 10.1016/j.xpro.2022.101753 Cátia F. Marques; Maria Matilde Marques; Gonçalo C. Justino. "Leukotrienes vs. Montelukast—Activity, Metabolism, and Toxicity Hints for Repurposing". Pharmaceuticals (2022): https://doi.org/10.3390/ph15091039. 10.3390/ph15091039 Marques, Catia F.; Marques, M. Matilde; Justino, Goncalo C.. "Leukotrienes vs. Montelukast - Activity, Metabolism, and Toxicity Hints for Repurposing". Preprints.org (2022): https://publons.com/wos-op/publon/56444618/. 10.20944/PREPRINTS202207.0379 Gonçalo C. Justino; Catarina P. Nascimento; Marta C. Justino. "Molecular dynamics simulations and analysis for bioinformatics undergraduate students". Biochemistry and Molecular Biology Education (2021): https://doi.org/10.1002/bmb.21512. 10.1002/bmb.21512 Justino, Gonçalo C.. "Immobilization of His-tagged proteins on NiO foams for recyclable enzymatic reactors". Applied Surface Science 537 (2021): 147848-147848. https://www.sciencedirect.com/science/article/pii/S0169433220326052. https://doi.org/10.1016/j.apsusc.2020.147848 Babu, K. Sowri; Pinheiro, Pedro F.; Marques, Catia F.; Justino, Goncalo C.; Andrade, Suzana M.; Alves, Marta M.. "Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization (vol 10, 15018, 2020)". Scientific Reports (2021): https://publons.com/wos-op/publon/51320663/. 10.1038/S41598-021-03382-W Marques, C. F.; Justino, G. C.; Marques, M. M.. "Potential pathways involved in Montelukast neurotoxicity: a metabolomics approach". Toxicology Letters (2021): https://publons.com/wos-op/publon/56444620/. Babu, K. Sowri; Pinheiro, Pedro F.; Marques, Cátia F.; Justino, Gonçalo C.; Andrade, Suzana M.; Alves, Marta M.. "Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-72133-0. 10.1038/s41598-020-72133-0 Pinheiro, Pedro F.; Justino, Gonçalo C.; Marques, M. Matilde. "NKp30 - A prospective target for new cancer immunotherapy strategies". British Journal of Pharmacology 177 20 (2020): 4563-4580. http://dx.doi.org/10.1111/bph.15222. 10.1111/bph.15222 Bento, Rafael; Marques, Catia; Bernardes, Carlos; Justino, Goncalo; Piedade, Manuel; Antunes, Fernando. "Metabolic Pathway Deficiencies are Compensated by Higher Energy Dissipation to Maintain Microbiological Growth rate in Saccharomyces cerevisiae". Free Radical Biology and Medicine (2020): https://publons.com/wos-op/publon/44163686/. 10.1016/J.FREERADBIOMED.2020.10.170 Mahgoub, Mohamed Y.; Elmaghraby, Awatef M.; Harb, Abd-Elfttah A.; Ferreira da Silva, João L.; Justino, Gonçalo C.; Marques, M. Matilde. "Synthesis, Crystal Structure, and Biological Evaluation of Fused Thiazolo[3,2-a]Pyrimidines as New Acetylcholinesterase Inhibitors". Molecules 24 12 (2019): 2306. http://dx.doi.org/10.3390/molecules24122306. 10.3390/molecules24122306 Marques, C. F.; Justino, G. C.; Gomes, C. A.; Marques, M. M.. "New insights into Montelukast metabolism - possible implications to the drug's adverse effects". Toxicology Letters (2019): https://publons.com/wos-op/publon/56444621/. Batuca, J.; Amaral, M.; Favas, C.; Justino, G.; Papoila, A.; Ames, P.; Delgado Alves, J.. "Antibodies against HDL components improve the diagnostic accuracy between ischemic stroke or coronary artery disease and healthy controls when added to traditional cardiovascular risk factors". Atherosclerosis 275 (2018): e88. http://dx.doi.org/10.1016/j.atherosclerosis.2018.06.241. 10.1016/j.atherosclerosis.2018.06.241 Batuca, Joana; Amaral, Marta; Favas, Catarina; Justino, Gonçalo; Papoila, Ana; Ames, Paul; Alves, José. "Antibodies against HDL Components in Ischaemic Stroke and Coronary Artery Disease". Thrombosis and Haemostasis 118 06 (2018): 1088-1100. http://dx.doi.org/10.1055/s-0038-1645857. 10.1055/s-0038-1645857 Pinheiro, Pedro; Justino, Goncalo; Miranda, Joana; Matilde Marques, M.. "Inducing the activity of NK cells with NKp30 small organic ligands". Abstracts of Papers of the American Chemical Society (2018): https://publons.com/wos-op/publon/14992591/. Correia, I.; Chorna, I.; Cavaco, I.; Roy, S.; Kuznetsov, M.L.; Ribeiro, N.; Justino, G.; et al. "Interaction of [VIVO(acac)2] with Human Serum Transferrin and Albumin". Chemistry - An Asian Journal 12 16 (2017): 2062-2084. http://www.scopus.com/inward/record.url?eid=2-s2.0-85025448120&partnerID=MN8TOARS. 10.1002/asia.201700469 Gonçalo C. Justino; Pedro F. Pinheiro; Alexandra P. S. Roseiro; Ana S. O. Knittel; João Gonçalves; Marta C. Justino; M. Fernanda N. N. Carvalho. "Camphor-based CCR5 blocker lead compounds – a computational and experimental approach". RSC Adv. 6 61 (2016): 56249-56259. http://dx.doi.org/10.1039/c6ra09627a. 10.1039/c6ra09627a Martins IL; Charneira C; Gandin V; Ferreira da Silva JL; Justino GC; Telo JP; Vieira AJ; et al. "Selenium-containing Chrysin and Quercetin Derivatives: Attractive scaffolds for cancer therapy.". Journal of Medicinal Chemistry 58 10 (2015): 4250-4265. http://europepmc.org/abstract/med/25906385. 10.1021/acs.jmedchem.5b00230 Pessoa, Joao Costa; Justino, Goncalo; Correia, Isabel; Roy, Somnath; Garribba, Eugenio. "Interaction of therapeutic vanadium complexes with serum proteins". Abstracts of Papers of the American Chemical Society (2014): https://publons.com/wos-op/publon/18465248/. Gonçalo Justino. "Binding of V(IV)O²¿ to the Fe binding sites of human serum transferrin. A theoretical study.". J Biol Inorg Chem 18 7 (2013): 803-813. http://dx.doi.org/10.1007/s00775-013-1029-x. 10.1007/s00775-013-1029-x Gonçalo Justino. "Electronic communication in linear oligo(azobenzene) radical anions.". J Phys Chem A 117 51 (2013): 14056-14064. http://dx.doi.org/10.1021/jp407099c. 10.1021/jp407099c Justino, Goncalo C.; Harjivan, Shrika G.; Marques, M. Matilde. "Metallocene derivatives as selective estrogen receptor modulators a computational docking to estrogen receptors a and b". Abstracts of Papers of the American Chemical Society (2011): https://publons.com/wos-op/publon/11121357/. Antunes, Alexandra M. M.; Justino, Goncalo C.; Martins, Ines L.; Charneira, Catarina R.; Marques, M. Matilde. "Computational assessment of the potential role of chalcogenated flavonoids as antioxidants, enzyme inhibitors and cell signalling modulators via computational analysis". Abstracts of Papers of the American Chemical Society (2011): https://publons.com/wos-op/publon/11121359/. Pinto, L.F.V.; Justino, G.C.; Vieira, A.J.S.C.; Prabhakar, S.; Lobo, A.M.. "Thermal rearrangement of an N-hydroxyimidazole thiocarbamoyl derivative as a simple entry into the 4-thioimidazole motif". Arkivoc 2010 5 (2010): 17-23. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953406068&partnerID=MN8TOARS. Justino, GC; Vieira, AJSC. "Antioxidant mechanisms of Quercetin and Myricetin in the gas phase and in solution - a comparison and validation of semi-empirical methods". Journal of Molecular Modeling 16 5 (2010): 863-876. 10.1007/s00894-009-0583-1 Santos, PMP; Silva, SAG; Justino, GC; Vieira, AJSC. "Demethylation of theophylline(1,3-dimethylxanthine) to 1-methylxanthine: the first step of an antioxidising cascade". Redox Report 15 3 (2010): 138-144. 10.1179/174329210X12650506623726 Antunes, AMM; Godinho, ALA; Martins, IL; Justino, GC; Beland, FA; Marques, MM. "Amino Acid Adduct Formation by the Nevirapine Metabolite, 12-Hydroxynevirapine-A Possible Factor in Nevirapine Toxicity". Chemical Research in Toxicology 23 5 (2010): 888-899. 10.1021/tx900443z Justino GC; Rodrigues M; Florêncio MH; Mira L. "Structure and antioxidant activity of brominated flavonols and flavanones.". (2009): http://europepmc.org/abstract/med/19708016. 10.1002/jms.1630 Justino, GC; Borges, CM; Florencio, MH. "Electrospray ionization tandem mass spectrometry fragmentation of protonated flavone and flavonol aglycones: a re-examination". Rapid Communications in Mass Spectrometry 23 2 (2009): 237-248. 10.1002/rcm.3869 Santos, MR; Rodriguez-Gomez, MJ; Justino, GC; Charro, N; Florencio, MH; Mira, L. "Influence of the metabolic profile on the in vivo antioxidant activity of quercetin under a low dosage oral regimen in rats". British Journal of Pharmacology 153 8 (2008): 1750-1761. 10.1038/bjp.2008.46 Justino, Goncalo C.; Rodrigues, Margarida; Florencio, M. Helena; Mira, Lurdes. "Flavonoid oxidation by the immune system: Structure-antioxidant activity of nitrated and chlorinated flavonoids". Free Radical Research (2007): https://publons.com/wos-op/publon/6613819/. Justino, Goncalo C.; Ferreira, Antonio. "The interaction of reactive species and plasmatic antioxidants - A tale of many tales". Free Radical Biology and Medicine (2007): https://publons.com/wos-op/publon/56444627/. Justino, Goncalo C.; Rodrigues, Margarida; Mira, Lurdes. "Hypobromous acid scavenging by flavonoids: Antioxidant activity of bromoflavonoids". Free Radical Biology and Medicine (2007): https://publons.com/wos-op/publon/56444623/. Justino, Goncalo; Rodrigues, Margarida; Mira, Lurdes. "Hyporbromous acid scavenging by flavonoids: Antioxidant activity of bromoflavonoids". Free Radical Research (2007): https://publons.com/wos-op/publon/56444622/. Rodrigues, Margarida; Freire, Andreia; Justino, Goncalo C.; Monteiro, Maria do Cue; Mira, Lurdes. "HOCI produced by human neutrophils scavenged by flavonoids". Free Radical Research (2007): https://publons.com/wos-op/publon/56444624/. Justino, GC; Correia, CF; Mira, L; Dos Santos, RMB; Simoes, JAM; Silva, AM; Santos, C; Gigante, B. "Antioxidant activity of a catechol derived from abietic acid". Journal of Agricultural and Food Chemistry 54 2 (2006): 342-348. 10.1021/jf052062k Justino, GC; Rodrigues, M; Mira, L. "The antioxidant activity of flavonoid halides and nitroflavonoids - Improving the response to chronic inflammation". Free Radical Research (2005): https://publons.com/wos-op/publon/56444626/. Justino GC; Santos MR; Canário S; Borges C; Florêncio MH; Mira L; Justino, G.C.; et al. "Plasma quercetin metabolites: structure-antioxidant activity relationships.". Archives of Biochemistry and Biophysics 432 1 (2004): 109-121. http://europepmc.org/abstract/med/15519302. 10.1016/j.abb.2004.09.007 Justino, GC; Mira, L; Ferreira, A. "On reactive species - A theoretical approach". Free Radical Biology and Medicine (2003): https://publons.com/wos-op/publon/56444625/. Silva, M.M.; Santos, M.R.; Caroço, G.; Rocha, R.; Justino, G.; Mira, L.; Silva, MM; et al. "Structure-antioxidant activity relationships of flavonoids: A re-examination". Free Radical Research 36 11 (2002): 1219-1227. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036827942&partnerID=MN8TOARS. 10.1080/198-1071576021000016472
Manual	Técnicas de Modelação Aplicadas à Biotecnologia. 2022. 10.5281/ZENODO.5979689 A concise introduction to MD simulations with GROMACS. 2022. 10.5281/ZENODO.5979655 Bioquímica Computacional - Aulas Práticas. 2022. 10.5281/ZENODO.5979639 Justino, Gonçalo C.. Sebenta de Química Orgânica. Barreiro, Portugal: Instituto Politécnico de Setúbal, Escola Superior de Tecnologia do Barreiro. 2018. Published Sebenta de Laboratórios IIIB. 2015. 10.5281/ZENODO.5979625 Química Orgânica. 2015. 10.5281/ZENODO.5979617
Preprint	Pedro Silva; Pedro Pinheiro; Sérgio P. Camões; Ana P.C. Ribeiro; Luísa Martins; Joana P. Miranda; Gonçalo C. Justino. "Exploring the Mechanisms Behind the Anti-tumoral Effects of Model C-scorpionate Complexes". 2023. https://doi.org/10.20944/preprints202306.0205.v1. 10.20944/preprints202306.0205.v1 Rafael N. Bento; Miguel A. Rendas; Valdir A. R. Semedo; Cátia F. Marques; Gonçalo. C. Justino; Carlos E. S. Bernardes; Manuel E. Minas da Piedade; Fernando Antunes. "Energy dissipation in early detection of cellular responses to metabolic challenges". 2022. https://doi.org/10.1101/2022.08.03.502646. 10.1101/2022.08.03.502646 Cátia F. Marques; M. Matilde Marques; Gonçalo C. Justino. "Leukotrienes vs. Montelukast – Activity, Metabolism, and Toxicity Hints for Repurposing". 2022. https://doi.org/10.20944/preprints202207.0379.v1. 10.20944/preprints202207.0379.v1
Thesis / Dissertation	Justino, Gonçalo C.. "Antioxidantes de Estrutura Catecólica - Metabolismo e Relações Estrutura- Actividade". PhD, Universidade de Lisboa Faculdade de Ciências, 2007.

Other

Dataset

Justino, Gonçalo C.. NKp30 -- a prospective target for new cancer immunotherapy strategies. 10.22541/au.158629969.98324039

Award

2010	Prémio NEDAI de Investigação em Auto-imunidade Sociedade Portuguesa de Medicina Interna, Portugal