1) Research: After his PhD in Würzburg and a DFG-funded postdoc at UNC Chapel Hill, M. Hof won a prestigious ¿Habilitations Liebig-Stipendium¿ in 1993. Rather than moving to one of the leading western universities, he decided due to family reasons to transfer the award to the Charles University in Prague. He was planning to launch an ambitious research program aimed at developing new fluorescence methods to study the blood coagulation process at the molecular level. Given the lamentable state of experimental equipment and support in freshly post-communist Czechia this was an almost impossible task. However, M. Hof succeeded in part due to his true grit and in part by performing parts of his research in Chicago (with Graham Fleming) and Würzburg (here he completed his habilitation in 1999). With conditions for science gradually improving in the Czech Republic, he accepted a position of group leader at the J. Heyrovsky Institute in 2000. His remarkable success in acquiring third-party funds allowed him to gradually build up his lab for development of novel fluorescence (F-) techniques. Development of F-techniques: Methods like the time-dependent fluorescence shift approach (TDFS) for membrane (e.g. Sykora Langmuir 2002) and enzyme (Jesenska JACS 2009) sciences, the first calibration-free Fluorescence Correlation Spectroscopy technique (z-scan FCS; Benda Langmuir 2003), F lifetime CS (FLCS; e.g. Benda Rev Sci Instr 2005), F Spectral Correlation Spectroscopy (Benda Optics Express 2014) and Dynamic Saturation Optical Microscopy (Humpolickova PCCP 2010) were developed in Prague. Moreover, he succeeded in advancing the following F-techniques: Fluorescence Lifetime Imaging Microscopy and Förster Resonance Energy Transfer method combined with Monte-Carlo simulations (e.g. Sachl Biophys J 2011), fluorescence antibunching for membrane-associated aggregation phenomena (Sachl BBA-Mol Cell Res 2015) and 2-foci FCS (Stefl Optics Express 2014). Recently, the interpretation of different fluorescence leakage assays was harmonised (Braun, ACS Nano 2018) and combined with single molecule number and brightness analysis (Steringer, eLife 2017; Sachl, Analytical Chem 2020) Applications of F-techniques: Concerning biophysics, M. Hof made fundamental contribution to the influence of solid support (e.g. Przbylo Langmuir 2006), ions (e.g. Melcerova Sci Rep 2016), oxidised lipids (e.g. Volinsky Biophys J 2011) and sterols (e.g. Kulig J Phys Chem Lett 2018), and lipid composition (Vinklarek J Phys Chem Lett 2019; Cebecauer Chem Rev 2018: Sarmento Biophys J 2021) on the physical chemical properties of lipid bilayers. In terms of application of these F-techniques in biology, the first usage of FLCS in living cells might serve as an interesting example (Huranova J Cell Biol 2010). In the last years, M. Hof further increased the complexity of the addressed questions. One can highlight his contributions to rational enzyme design (Sykora Nat Chem Biol 2014; Amaro JACS 2015; Kokonnen JACS 2018), applications of TDFS in DNA and protein science (Dziuba Chemical Sciences 2016; Fischermeier, Angewandte 2017), application of new molecular rotors in live cell imaging (Dziuba Angewandte 2016; Hot paper), the single-molecule studies on the membrane-mediated oligomerisations of the ß-amyloid peptide (Amaro Angewandte 2016; VIP paper) or of the FGF2 protein (Steringer Elife 2017 and Sachl Anal Chemie 2020). 2) Supervision of graduate students and postdoctoral fellows and teaching activities 2001- advisor or co-advisor of 15 Postdocs/ 27 PhD students/ 7 Master students at the J. Heyrovský Institute, in the framework of study programs of the above-mentioned Czech Universities; Teaching regular courses in spectroscopy and microscopy at those universities. 1993-2000 co-advisor of 3 PhD Students (Department of Physical Chemistry, Würzburg); Teaching regular courses in physical chemistry at the Faculty of Chemistry and Pharmacy, Würzburg