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Identificação

Identificação pessoal

Nome completo
Masahito Asada

Nomes de citação

  • Asada, Masahito

Identificadores de autor

Ciência ID
8013-ED55-300D
ORCID iD
0000-0003-0042-6856
Formação
Grau Classificação
2006/04/01 - 2010/03/31
Concluído
 Graduate School of Agricultural and Life Sciences (Doctor of Philosophy)
Especialização em Veterinary Science
Tokyo Daigaku Daigakuin Nogaku Seimei Kagaku Kenkyuka Nogakubu, Japão
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2010/04/01 - 2013/01/31 Pós-doutorado (Investigação) Obihiro Chikusan Daigaku Genchubyo Kenkyu Center, Japão
Obihiro Chikusan Daigaku Genchubyo Kenkyu Center, Japão

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento 		Empregador
2019/11/01 - Atual Professor Associado (Docente Universitário) Obihiro Chikusan Daigaku Genchubyo Kenkyu Center, Japão
Obihiro Chikusan Daigaku Genchubyo Kenkyu Center, Japão
2013/02/01 - 2019/10/31 Professor Auxiliar (Docente Universitário) Nagasaki Daigaku Nettai Igaku Kenkyujo, Japão
Nagasaki Daigaku Nettai Igaku Kenkyujo, Japão
Projetos

Bolsa

Designação Financiadores
2019/04 - 2022/03 Signaling mechanism of the apical organelle discharge by malaria parasites during erythrocyte invasion
19H03461
 Nihon Gakujutsu Shinkokai
2019/04 - 2022/03 Comprehensive analysis of Babesia tick-stage antigens: Establishing of a base for development the TBV
19H03120
 Nihon Gakujutsu Shinkokai
2019/04 - 2022/03 ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿·¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿
19K06384
 Nihon Gakujutsu Shinkokai
2018/06 - 2020/03 Studies on development of the Babesia parasites that express cytokine for non-specific immune activation
18K19258
 Nihon Gakujutsu Shinkokai
2016/04 - 2019/03 ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿
16H05807
 Nihon Gakujutsu Shinkokai
2016/04 - 2019/03 ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿
16K08021
 Nihon Gakujutsu Shinkokai
2016/04 - 2019/03 ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿
16H05184
 Nihon Gakujutsu Shinkokai
2013/04 - 2016/03 Bioimaging analysis on erythrocyte invasion mechanisms of Babesia bovis
25850199
 Nihon Gakujutsu Shinkokai
2013/04 - 2016/03 Signal transduction during erythrocyte invasion by malaria parasites
25293102
 Nihon Gakujutsu Shinkokai
2012/04 - 2016/03 Seroepidemiology of the molecules expressed on the Plasmodium vivax-infected erythrocyte surface
24406012
 Nihon Gakujutsu Shinkokai
Produções

Publicações

Artigo em revista
  1. Kittisak Thawnashom; Luzia Helena Carvalho; Miho Kaneko; Phonepadith Xangsayarath; Nattawat Chaiyawong; Kazuhide Yahata; Masahito Asada; John H. Adams; Osamu Kaneko. "Validation of Plasmodium vivax centromere and promoter activities using Plasmodium yoelii". PLOS ONE 14 12 (2019): e0226884-e0226884. https://doi.org/10.1371/journal.pone.0226884.
    10.1371/journal.pone.0226884
  2. Asada, Masahito. "Genome Editing of Babesia bovis Using the CRISPR/Cas9 System.". mSphere (2019): http://europepmc.org/articles/PMC6563353.
    10.1128/mSphere.00109-19
  3. Nguyen, T.-T.; Dang-Trinh, M.-A.; Higuchi, L.; Mosqueda, J.; Hakimi, H.; Asada, M.; Yamagishi, J.; Umemiya-Shirafuji, R.; Kawazu, S.-I.. "Initiated Babesia ovata sexual stages under in vitro conditions were recognized by anti-CCp2 antibodies, showing changes in the DNA content by imaging flow cytometry". Pathogens 8 3 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85070574141&partnerID=MN8TOARS.
    10.3390/pathogens8030104
  4. Kandel, R.C.; Shrestha, M.; Sadaula, A.; KC, M.; Maharjan, J.; Solanki, G.S.; Chalise, M.K.; et al. "First report of malaria parasites in water buffalo in Nepal". Veterinary Parasitology: Regional Studies and Reports 18 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85074578562&partnerID=MN8TOARS.
    10.1016/j.vprsr.2019.100348
  5. Hakimi, H.; Sarani, A.; Takeda, M.; Kaneko, O.; Asada, M.. "Epidemiology, risk factors, and co-infection of vector-borne pathogens in goats from Sistan and Baluchestan province, Iran". PLoS ONE 14 6 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85067447248&partnerID=MN8TOARS.
    10.1371/journal.pone.0218609
  6. Rosa, C.; Asada, M.; Hakimi, H.; Domingos, A.; Pimentel, M.; Antunes, S.. "Transient transfection of Babesia ovis using heterologous promoters". Ticks and Tick-borne Diseases 10 6 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85071481258&partnerID=MN8TOARS.
    10.1016/j.ttbdis.2019.101279
  7. Asare, K.K.; Sakaguchi, M.; Lucky, A.B.; Asada, M.; Miyazaki, S.; Katakai, Y.; Kawai, S.; et al. "The Plasmodium knowlesi MAHRP2 ortholog localizes to structures connecting Sinton Mulligan's clefts in the infected erythrocyte". Parasitology International 67 4 (2018): 481-492. http://www.scopus.com/inward/record.url?eid=2-s2.0-85046727092&partnerID=MN8TOARS.
    10.1016/j.parint.2018.04.005
  8. Asada, M.; Hakimi, H.; Kawazu, S.-I.. "The application of the HyPer fluorescent sensor in the real-time detection of H2O2 in Babesia bovis merozoites in vitro". Veterinary Parasitology 255 (2018): 78-82. http://www.scopus.com/inward/record.url?eid=2-s2.0-85044964860&partnerID=MN8TOARS.
    10.1016/j.vetpar.2018.03.016
  9. Kaewthamasorn, M.; Takeda, M.; Saiwichai, T.; Gitaka, J.N.; Tiawsirisup, S.; Imasato, Y.; Mossaad, E.; et al. "Genetic homogeneity of goat malaria parasites in Asia and Africa suggests their expansion with domestic goat host". Scientific Reports 8 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85045404571&partnerID=MN8TOARS.
    10.1038/s41598-018-24048-0
  10. Liu, M.; Adjou Moumouni, P.F.; Asada, M.; Hakimi, H.; Masatani, T.; Vudriko, P.; Lee, S.-H.; et al. "Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni". Parasites and Vectors 11 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85045873040&partnerID=MN8TOARS.
    10.1186/s13071-018-2853-1
  11. Liu, M.; Adjou Moumouni, P.F.; Cao, S.; Asada, M.; Wang, G.; Gao, Y.; Guo, H.; et al. "Identification and characterization of interchangeable cross-species functional promoters between Babesia gibsoni and Babesia bovis". Ticks and Tick-borne Diseases 9 2 (2018): 330-333. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034750555&partnerID=MN8TOARS.
    10.1016/j.ttbdis.2017.11.008
  12. Asada, M.; Takeda, M.; Tomas, W.M.; Pellegrin, A.; de Oliveira, C.H.S.; Barbosa, J.D.; da Silveira, J.A.G.; Braga, É.M.; Kaneko, O.. "Close relationship of Plasmodium sequences detected from South American pampas deer (Ozotoceros bezoarticus) to Plasmodium spp. in North American white-tailed deer". International Journal for Parasitology: Parasites and Wildlife 7 1 (2018): 44-47. http://www.scopus.com/inward/record.url?eid=2-s2.0-85042639206&partnerID=MN8TOARS.
    10.1016/j.ijppaw.2018.01.001
  13. Kegawa, Y.; Asada, M.; Ishizaki, T.; Yahata, K.; Kaneko, O.. "Critical role of Erythrocyte Binding-Like protein of the rodent malaria parasite Plasmodium yoelii to establish an irreversible connection with the erythrocyte during invasion". Parasitology International 67 6 (2018): 706-714. http://www.scopus.com/inward/record.url?eid=2-s2.0-85050632175&partnerID=MN8TOARS.
    10.1016/j.parint.2018.07.006
  14. Yamagishi, J.; Asada, M.; Hakimi, H.; Tanaka, T.Q.; Sugimoto, C.; Kawazu, S.-I.. "Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens". BMC Genomics 18 1 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85032393520&partnerID=MN8TOARS.
    10.1186/s12864-017-4230-4
  15. Liu, M.; Asada, M.; Cao, S.; Adjou Moumouni, P.F.; Vudriko, P.; Efstratiou, A.; Hakimi, H.; et al. "Transient transfection of intraerythrocytic Babesia gibsoni using elongation factor-1 alpha promoter". Molecular and Biochemical Parasitology 216 (2017): 56-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-85025080486&partnerID=MN8TOARS.
    10.1016/j.molbiopara.2017.07.003
  16. Masatani, T.; Hayashi, K.; Andoh, M.; Tateno, M.; Endo, Y.; Asada, M.; Kusakisako, K.; et al. "Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan". Ticks and Tick-borne Diseases 8 4 (2017): 581-587. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019104532&partnerID=MN8TOARS.
    10.1016/j.ttbdis.2017.03.007
  17. Yamasaki, S.; Suganuma, K.; Yamagishi, J.; Asada, M.; Yokoyama, N.; Kawazu, S.-I.; Inoue, N.. "Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense". Parasites and Vectors 9 1 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84969795495&partnerID=MN8TOARS.
    10.1186/s13071-016-1563-9
  18. Masatani, T.; Asada, M.; Hakimi, H.; Hayashi, K.; Yamagishi, J.; Kawazu, S.-I.; Xuan, X.. "Identification and functional analysis of a novel mitochondria-localized 2-Cys peroxiredoxin, BbTPx-2, from Babesia bovis". Parasitology Research 115 8 (2016): 3139-3145. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964389903&partnerID=MN8TOARS.
    10.1007/s00436-016-5071-9
  19. Templeton, T.J.; Asada, M.; Jiratanh, M.; Ishikawa, S.A.; Tiawsirisup, S.; Sivakumar, T.; Namangala, B.; et al. "Ungulate malaria parasites". Scientific Reports 6 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84961851538&partnerID=MN8TOARS.
    10.1038/srep23230
  20. Hakimi, H.; Yamagishi, J.; Kegawa, Y.; Kaneko, O.; Kawazu, S.-I.; Asada, M.. "Establishment of transient and stable transfection systems for Babesia ovata". Parasites and Vectors 9 1 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84966415690&partnerID=MN8TOARS.
    10.1186/s13071-016-1439-z
  21. Musinguzi, S.P.; Suganuma, K.; Asada, M.; Laohasinnarong, D.; Sivakumar, T.; Yokoyama, N.; Namangala, B.; et al. "A PCR-based survey of animal African trypanosomosis and selected piroplasm parasites of cattle and goats in Zambia". Journal of Veterinary Medical Science 78 12 (2016): 1819-1824. http://www.scopus.com/inward/record.url?eid=2-s2.0-85009227688&partnerID=MN8TOARS.
    10.1292/jvms.16-0240
  22. Laohasinnarong, D.; Goto, Y.; Asada, M.; Nakao, R.; Hayashida, K.; Kajino, K.; Kawazu, S.-I.; et al. "Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia". Parasites and Vectors 8 1 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84942593853&partnerID=MN8TOARS.
    10.1186/s13071-015-1112-y
  23. Mossaad, E.; Asada, M.; Nakatani, D.; Inoue, N.; Yokoyama, N.; Kaneko, O.; Kawazu, S.-I.. "Calcium ions are involved in egress of Babesia bovis merozoites from bovine erythrocytes". Journal of Veterinary Medical Science 77 1 (2015): 53-58. http://www.scopus.com/inward/record.url?eid=2-s2.0-84922979142&partnerID=MN8TOARS.
    10.1292/jvms.14-0391
  24. Asada, M.; Yahata, K.; Hakimi, H.; Yokoyama, N.; Igarashi, I.; Kaneko, O.; Suarez, C.E.; Kawazu, S.-I.. "Transfection of Babesia bovis by double selection with WR99210 and blasticidin-S and its application for functional analysis of thioredoxin peroxidase-1". PLoS ONE 10 5 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84930629457&partnerID=MN8TOARS.
    10.1371/journal.pone.0125993
  25. Hakimi, H.; Suganuma, K.; Usui, M.; Masuda-Suganuma, H.; Angeles, J.M.M.; Asada, M.; Kawai, S.; Inoue, N.; Kawazu, S.-I.. "Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity". Parasitology Research 113 11 (2014): 3957-3962. http://www.scopus.com/inward/record.url?eid=2-s2.0-84919382912&partnerID=MN8TOARS.
    10.1007/s00436-014-4060-0
  26. Masatani, T.; Asada, M.; Ichikawa-Seki, M.; Usui, M.; Terkawi, M.A.; Hayashi, K.; Kawazu, S.-I.; Xuan, X.. "Cloning and characterization of a 2-Cys peroxiredoxin from Babesia gibsoni". Journal of Veterinary Medical Science 76 1 (2014): 139-143. http://www.scopus.com/inward/record.url?eid=2-s2.0-84893507884&partnerID=MN8TOARS.
    10.1292/jvms.13-0274
  27. Angeles, J.M.M.; Kirinoki, M.; Goto, Y.; Asada, M.; Hakimi, H.; Leonardo, L.R.; Tongol-Rivera, P.; et al. "Localization and expression profiling of a 31 kDa antigenic repetitive protein Sjp-0110390 in Schistosoma japonicum life stages". Molecular and Biochemical Parasitology 187 2 (2013): 98-102. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874743931&partnerID=MN8TOARS.
    10.1016/j.molbiopara.2012.12.002
  28. Yoshinari, T.; Sivakumar, T.; Asada, M.; Battsetseg, B.; Huang, X.; Lan, D.T.B.; Inpankaew, T.; et al. "A PCR based survey of Babesia ovata in cattle from various Asian, African and South American Countries". Journal of Veterinary Medical Science 75 2 (2013): 211-214. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874718737&partnerID=MN8TOARS.
    10.1292/jvms.12-0329
  29. Masatani, T.; Ooka, H.; Terkawi, M.A.; Cao, S.; Luo, Y.; Asada, M.; Hayashi, K.; Nishikawa, Y.; Xuan, X.. "Identification, cloning and characterization of BmP41, a common antigenic protein of Babesia microti". Journal of Veterinary Medical Science 75 7 (2013): 967-970. http://www.scopus.com/inward/record.url?eid=2-s2.0-84880927161&partnerID=MN8TOARS.
    10.1292/jvms.12-0535
  30. Suganuma, K.; Mochabo, K.M.; Hakimi, H.; Yamasaki, S.; Yamagishi, J.; Asada, M.; Kawazu, S.-I.; Inoue, N.. "Adenosine-uridine-rich element is one of the required cis-elements for epimastigote form stage-specific gene expression of the congolense epimastigote specific protein". Molecular and Biochemical Parasitology 191 1 (2013): 36-43. http://www.scopus.com/inward/record.url?eid=2-s2.0-84884522544&partnerID=MN8TOARS.
    10.1016/j.molbiopara.2013.09.001
  31. Terkawi, M.A.; Ratthanophart, J.; Salama, A.; AbouLaila, M.; Asada, M.; Ueno, A.; Alhasan, H.; et al. "Molecular characterization of a new Babesia bovis thrombospondin-related anonymous protein (BbTRAP2)". PLoS ONE 8 12 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84892639182&partnerID=MN8TOARS.
    10.1371/journal.pone.0083305
  32. Hakimi, H.; Asada, M.; Angeles, J.M.M.; Kawai, S.; Inoue, N.; Kawazu, S.-I.. "Plasmodium vivax and Plasmodium knowlesi: Cloning, expression and functional analysis of 1-Cys peroxiredoxin". Experimental Parasitology 133 1 (2013): 101-105. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873949791&partnerID=MN8TOARS.
    10.1016/j.exppara.2012.10.018
  33. Hakimi, H.; Asada, M.; Angeles, J.M.M.; Inoue, N.; Kawazu, S.-I.. "Cloning and characterization of Plasmodium vivax thioredoxin peroxidase-1". Parasitology Research 111 2 (2012): 525-529. http://www.scopus.com/inward/record.url?eid=2-s2.0-84866045554&partnerID=MN8TOARS.
    10.1007/s00436-012-2864-3
  34. Asada, M.; Goto, Y.; Yahata, K.; Yokoyama, N.; Kawai, S.; Inoue, N.; Kaneko, O.; Kawazu, S.-I.. "Gliding motility of babesia bovis merozoites visualized by time-lapse video microscopy". PLoS ONE 7 4 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84859624477&partnerID=MN8TOARS.
    10.1371/journal.pone.0035227
  35. Suganuma, K.; Yamasaki, S.; Asada, M.; Kawazu, S.-I.; Inoue, N.. "The epimastigote stage-specific gene expression of CESP is tightly regulated by its 3' UTR". Molecular and Biochemical Parasitology 186 1 (2012): 77-80. http://www.scopus.com/inward/record.url?eid=2-s2.0-84868476529&partnerID=MN8TOARS.
    10.1016/j.molbiopara.2012.09.010
  36. Angeles, J.M.M.; Goto, Y.; Kirinoki, M.; Asada, M.; Leonardo, L.R.; Rivera, P.T.; Villacorte, E.A.; et al. "Utilization of ELISA Using Thioredoxin Peroxidase-1 and Tandem Repeat Proteins for Diagnosis of Schistosoma japonicum Infection among Water Buffaloes". PLoS Neglected Tropical Diseases 6 8 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84865984566&partnerID=MN8TOARS.
    10.1371/journal.pntd.0001800
  37. Asada, M.; Tanaka, M.; Goto, Y.; Yokoyama, N.; Inoue, N.; Kawazu, S.-I.. "Stable expression of green fluorescent protein and targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis with the WR99210/dhfr selection system". Molecular and Biochemical Parasitology 181 2 (2012): 162-170. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856231102&partnerID=MN8TOARS.
    10.1016/j.molbiopara.2011.11.001
  38. Goto, Y.; Duthie, M.S.; Nguyen, T.-T.; Asada, M.; Kawazu, S.-I.; Carter, D.; Inoue, N.. "Serological characterizations of tandem repeat proteins for detection of African trypanosome infection in cattle". Parasitology International 60 4 (2011): 538-540. http://www.scopus.com/inward/record.url?eid=2-s2.0-82155172905&partnerID=MN8TOARS.
    10.1016/j.parint.2011.09.003
  39. Ozbel, Y.; Sanjoba, C.; Alten, B.; Asada, M.; Depaquit, J.; Matsumoto, Y.; Demir, S.; Siyambalagoda, R.R.M.L.R.; Rajapakse, R.P.V.J.. "Distribution and ecological aspects of sand fly (Diptera: Psychodidae) species in Sri Lanka". Journal of Vector Ecology 36 SUPPL.1 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79952126430&partnerID=MN8TOARS.
    10.1111/j.1948-7134.2011.00115.x
  40. Goto, Y.; Sanjoba, C.; Asada, M.; Saeki, K.; Onodera, T.; Matsumoto, Y.. "Adhesion of MRP8/14 to amastigotes in skin lesions of Leishmania major-infected mice". Experimental Parasitology 119 1 (2008): 80-86. http://www.scopus.com/inward/record.url?eid=2-s2.0-41349119568&partnerID=MN8TOARS.
    10.1016/j.exppara.2007.12.019
Capítulo de livro
  1. Matsumoto, Y.; Sanjoba, C.; Asada, M.; Osada, Y.. "Polymorphism of leishmaniasis caused by Leishmania donovani Sensu Lato in Asia". 101-110. 2011.